Processing fastq files for the sample - UG10S1P011 Reading 5347_7332_21152_HLKNYBGXX_UG10S1P011_R1.fastq.gz 5347_7332_21152_HLKNYBGXX_UG10S1P011_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21152\tSM:UG10S1P011\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21152_HLKNYBGXX_UG10S1P011_R1.fastq.gz 5347_7332_21152_HLKNYBGXX_UG10S1P011_R2.fastq.gz | samtools view -Sb1 - > UG10S1P011_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10875, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (123.60, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (241.65, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.352 CPU sec, 6.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10823, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 128, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (149.00, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (239.00, 122.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.163 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11057, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 128, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (149.89, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.64, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.791 CPU sec, 5.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11139, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 153, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (142.43, 81.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (241.24, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.246 CPU sec, 6.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11099, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (130.33, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (244.27, 127.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.390 CPU sec, 5.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11190, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 165, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (151.94, 63.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (245.24, 127.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.609 CPU sec, 6.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11193, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 240, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (231.57, 159.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (241.89, 125.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.556 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11278, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 186, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (209.71, 143.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (245.27, 128.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.028 CPU sec, 5.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11192, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 241, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (196.13, 96.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.25, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.002 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11236, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 101, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (141.47, 96.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.00, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.367 CPU sec, 5.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11125, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 145, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (157.40, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (244.48, 126.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.162 CPU sec, 5.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11071, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (244.12, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.776 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11125, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 149, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (160.50, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.72, 125.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.446 CPU sec, 6.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11122, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 179, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (170.47, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (243.11, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.399 CPU sec, 7.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11171, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (173.94, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 212, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (244.55, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.063 CPU sec, 6.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11045, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (161.21, 59.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (243.50, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.005 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11263, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 145, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (182.18, 112.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (242.28, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.162 CPU sec, 5.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11326, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 115, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (160.61, 105.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.17, 124.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.858 CPU sec, 6.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11015, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 228, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (220.30, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.15, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.469 CPU sec, 6.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11162, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 145, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (150.38, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.38, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.775 CPU sec, 6.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11098, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 147, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (144.94, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.30, 123.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.771 CPU sec, 6.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11285, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (210.21, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (236.06, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.229 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11243, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 153, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (167.32, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.13, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.717 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11351, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 153, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (157.08, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.52, 122.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.337 CPU sec, 5.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11245, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 166, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (182.38, 109.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.63, 120.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.941 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11321, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 178, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (190.15, 102.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.77, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.598 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11317, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 140, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (131.33, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.48, 122.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.833 CPU sec, 5.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11136, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (140.93, 72.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (237.14, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.074 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11030, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 141, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (152.42, 94.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (239.56, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.701 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10864, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 162, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (229.44, 235.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (242.10, 124.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.859 CPU sec, 5.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10802, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 85, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (125.73, 89.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.66, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.975 CPU sec, 6.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 10990, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 206, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (202.81, 88.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.73, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.829 CPU sec, 5.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11068, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (160.23, 113.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.41, 125.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.904 CPU sec, 5.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11163, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 159, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (159.79, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (241.88, 124.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.046 CPU sec, 5.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10994, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 160, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (193.91, 110.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.04, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.317 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11254, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 168, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (159.00, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (244.36, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.385 CPU sec, 5.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11447, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 134, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (160.06, 104.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.23, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.583 CPU sec, 5.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11398, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 196, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (203.56, 102.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (243.74, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.512 CPU sec, 5.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11307, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 118, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (142.16, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.00, 127.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.420 CPU sec, 7.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11132, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (146.18, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (240.97, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.295 CPU sec, 6.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11245, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (143.76, 107.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (244.43, 126.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.692 CPU sec, 6.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11265, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 153, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (174.78, 98.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (243.39, 125.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.581 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11148, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 178, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (149.38, 70.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (241.31, 123.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.275 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11072, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 184, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (211.94, 132.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (239.92, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.202 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11143, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (138.60, 57.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (244.73, 124.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.811 CPU sec, 5.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11200, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 217, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (241.11, 159.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.04, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.259 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11219, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 174, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (189.00, 107.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.48, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.366 CPU sec, 5.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11233, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 168, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (161.00, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (240.83, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.759 CPU sec, 5.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11379, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 135, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (168.08, 106.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (238.02, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.387 CPU sec, 5.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11197, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 164, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (191.94, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.27, 121.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.335 CPU sec, 5.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11257, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (132.97, 62.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.36, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.931 CPU sec, 5.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11315, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (168.24, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (234.41, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.991 CPU sec, 5.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11056, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 128, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (188.80, 124.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.97, 120.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.047 CPU sec, 5.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11208, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 182, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (169.00, 76.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.34, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.316 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11137, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 135, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (138.07, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (233.97, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.061 CPU sec, 5.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11235, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 111, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (148.26, 108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.86, 120.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.679 CPU sec, 5.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11181, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 89, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (161.94, 139.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (233.58, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.388 CPU sec, 6.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 10993, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 144, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (150.71, 78.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (240.97, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.401 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 10901, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 137, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (163.80, 81.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (240.35, 122.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.597 CPU sec, 5.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11038, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 133, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (144.20, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.44, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.314 CPU sec, 6.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11094, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (161.28, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (240.05, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.870 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11070, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 155, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (134.36, 64.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (242.18, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.339 CPU sec, 5.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11149, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 185, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (205.40, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (240.34, 124.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.617 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11156, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 176, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (154.06, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (241.40, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.884 CPU sec, 6.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11091, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 122, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (156.21, 92.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.80, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.894 CPU sec, 5.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11261, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 140, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (145.89, 66.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (243.01, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.270 CPU sec, 6.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11301, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 178, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (201.00, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.43, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.146 CPU sec, 5.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11235, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 118, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (155.95, 119.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (242.76, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.892 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11233, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 125, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.06, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (240.69, 125.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.235 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11054, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 143, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (157.44, 84.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.75, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.070 CPU sec, 5.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11057, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 143, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (159.80, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.78, 126.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.011 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11120, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 170, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (189.39, 121.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.67, 123.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.381 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10993, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 131, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (179.53, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.28, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.598 CPU sec, 7.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10948, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 156, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (164.18, 107.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.79, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.650 CPU sec, 5.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11030, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 195, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (182.29, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (238.85, 122.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.029 CPU sec, 6.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11088, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 95, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (105.20, 54.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (237.00, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.608 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10968, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 139, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (131.28, 59.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.84, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.090 CPU sec, 5.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11227, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 136, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (190.32, 113.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (238.90, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.652 CPU sec, 6.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11257, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 176, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (232.20, 157.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.93, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.276 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11139, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 148, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (195.54, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.98, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.502 CPU sec, 6.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11235, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 115, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (134.77, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.66, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.079 CPU sec, 5.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11266, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 182, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (163.80, 106.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.02, 119.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.371 CPU sec, 6.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11149, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (190.08, 94.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (233.23, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.702 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11161, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 91, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (135.89, 92.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.95, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.011 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11138, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 134, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (156.44, 109.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.75, 120.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.753 CPU sec, 5.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10930, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (145.88, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.08, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.304 CPU sec, 6.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10906, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.84, 118.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.796 CPU sec, 5.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11027, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 128, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (159.80, 117.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.87, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.597 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11022, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (167.06, 112.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.60, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.569 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11169, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 159, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (142.40, 61.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.57, 120.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.585 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11227, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 105, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (147.68, 85.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (239.90, 123.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.431 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11138, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 92, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (107.23, 63.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (240.32, 123.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.859 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11058, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 183, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (249.42, 225.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (240.88, 123.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.150 CPU sec, 6.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11144, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 162, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (173.10, 107.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (241.56, 125.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.236 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11041, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 142, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (148.21, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.29, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.124 CPU sec, 5.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11177, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 196, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (190.00, 69.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.54, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.371 CPU sec, 5.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11290, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 161, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (145.40, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (242.51, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.247 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11296, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 103, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (141.86, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.63, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.788 CPU sec, 6.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11127, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (174.61, 102.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.39, 123.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.830 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11016, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (187.33, 118.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.79, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.376 CPU sec, 6.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11072, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 140, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (150.06, 107.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.35, 122.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.685 CPU sec, 6.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11159, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 123, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (158.63, 81.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.06, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.443 CPU sec, 7.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11070, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 149, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (140.06, 53.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (238.84, 122.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.374 CPU sec, 5.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11130, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 134, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (126.86, 67.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (236.27, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.220 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11130, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (148.62, 114.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (238.29, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.329 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10976, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 209, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (193.55, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.63, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.519 CPU sec, 7.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11104, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 75, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (73.44, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.89, 118.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.644 CPU sec, 6.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11202, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 187, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.79, 77.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (236.09, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.902 CPU sec, 5.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11197, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 152, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (200.67, 145.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (233.91, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.160 CPU sec, 5.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11147, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 146, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (148.87, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (234.17, 118.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.507 CPU sec, 5.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11087, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (169.68, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.71, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.171 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11073, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 155, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (173.88, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.15, 119.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.749 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11125, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 215, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (190.36, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (233.63, 118.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.865 CPU sec, 5.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11082, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (190.32, 109.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.10, 118.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.632 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10903, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 153, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (154.77, 77.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (228.67, 114.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.740 CPU sec, 5.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10897, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 162, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (160.38, 99.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (230.98, 117.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.479 CPU sec, 7.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11053, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 156, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (182.65, 115.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (233.01, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.670 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11107, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 115, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (124.76, 57.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (234.39, 120.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.309 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11125, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 162, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (162.90, 100.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.47, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.022 CPU sec, 6.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11036, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 148, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (173.00, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.47, 122.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.804 CPU sec, 6.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11126, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 106, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (124.42, 77.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.72, 123.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.454 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11163, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 156, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (183.12, 111.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.10, 122.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.239 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11230, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (182.57, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (240.14, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.725 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11187, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 210, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (199.63, 132.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.37, 125.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.786 CPU sec, 5.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11061, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 264, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (239.14, 93.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.84, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.217 CPU sec, 6.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11203, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 177, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (190.17, 79.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.45, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.770 CPU sec, 6.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11282, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 135, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (157.94, 93.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (239.64, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.789 CPU sec, 5.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11038, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 142, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (163.52, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (240.61, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.500 CPU sec, 6.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11183, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (239.39, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.439 CPU sec, 5.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11202, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 123, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (156.95, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.42, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.734 CPU sec, 6.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11206, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (146.95, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.66, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.689 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11181, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (179.06, 105.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (238.98, 123.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.938 CPU sec, 5.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11029, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 170, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (172.05, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (238.07, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.309 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11170, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 98, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (127.33, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.40, 121.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.525 CPU sec, 7.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11257, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 155, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (171.29, 100.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.25, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.731 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11138, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 156, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (156.24, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.25, 119.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.704 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11161, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 211, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (192.36, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.30, 118.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.700 CPU sec, 6.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11145, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (166.33, 90.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (233.94, 118.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.370 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11081, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 160, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (197.22, 132.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (233.24, 118.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.757 CPU sec, 5.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 11212, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 130, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (154.12, 105.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (233.00, 118.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.722 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11137, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 142, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (119.29, 48.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (232.96, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.042 CPU sec, 5.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11089, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 172, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (153.53, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.67, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.364 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11169, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (160.95, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.78, 119.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.999 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10872, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 193, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (179.50, 99.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.67, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.348 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10875, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 216, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (210.56, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (231.65, 117.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.230 CPU sec, 6.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10947, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 184, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (232.21, 119.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.86, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.898 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 10989, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (164.38, 115.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.15, 120.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.143 CPU sec, 5.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11020, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 231, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (216.93, 114.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.47, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.996 CPU sec, 5.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11087, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (206.89, 101.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.46, 121.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.586 CPU sec, 5.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11015, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 177, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (175.25, 91.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.63, 122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.334 CPU sec, 5.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11117, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 143, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (182.22, 145.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.32, 122.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.284 CPU sec, 6.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11127, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 204, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (191.17, 133.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.93, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.122 CPU sec, 6.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11029, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (164.06, 109.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (238.24, 122.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.232 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11137, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 190, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (188.61, 119.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.04, 122.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.684 CPU sec, 6.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11044, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 167, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (197.53, 94.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.83, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.694 CPU sec, 6.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11027, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (155.00, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (236.28, 120.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.460 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11079, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 114, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (136.62, 72.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.36, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.336 CPU sec, 7.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11029, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 104, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (120.83, 50.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.80, 122.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.004 CPU sec, 6.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11012, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 174, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (158.18, 74.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (236.09, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.576 CPU sec, 6.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10962, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (151.11, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.59, 121.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.891 CPU sec, 6.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11136, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 136, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (127.16, 46.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.83, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.935 CPU sec, 6.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11037, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (151.50, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.58, 120.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.544 CPU sec, 6.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10957, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 212, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (201.00, 144.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.95, 121.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.887 CPU sec, 6.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11039, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 174, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (157.57, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (234.19, 119.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.677 CPU sec, 6.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10942, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 172, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (164.24, 72.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.50, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.860 CPU sec, 6.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11171, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 119, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (131.79, 68.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (231.53, 116.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.933 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11143, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 111, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (145.54, 91.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.45, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.142 CPU sec, 5.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11106, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 176, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (161.74, 83.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (230.09, 115.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.352 CPU sec, 5.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11060, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 120, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (125.23, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (229.46, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.335 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 11130, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 164, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (178.51, 117.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.34, 117.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.985 CPU sec, 5.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11040, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 188, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (207.17, 102.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.92, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.648 CPU sec, 5.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11138, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 219, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (288.80, 236.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.80, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.290 CPU sec, 5.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10917, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 228, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (204.46, 178.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.43, 124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.467 CPU sec, 6.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11216, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 144, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (171.35, 104.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (240.21, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.614 CPU sec, 5.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11154, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 191, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (202.88, 96.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (242.02, 126.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.095 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11256, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 156, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (165.88, 100.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (242.50, 123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.761 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11354, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (132.59, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (245.27, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.150 CPU sec, 5.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10867, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 161, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (203.08, 120.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (244.88, 126.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.260 CPU sec, 5.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11090, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (118.65, 40.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.82, 124.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.114 CPU sec, 6.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11259, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 168, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (166.06, 79.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (243.16, 124.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.424 CPU sec, 5.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11215, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 125, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (130.29, 76.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (243.39, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.244 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11122, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (244.73, 126.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.348 CPU sec, 6.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11119, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (178.68, 96.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.12, 123.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.597 CPU sec, 5.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11012, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 159, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (127.89, 52.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (245.36, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.628 CPU sec, 5.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11051, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (151.62, 96.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (244.09, 125.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.667 CPU sec, 5.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10956, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 313) [M::mem_pestat] mean and std.dev: (159.12, 60.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (240.78, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.925 CPU sec, 6.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11152, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 154, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (180.61, 158.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.65, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.499 CPU sec, 6.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11314, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 119, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (223.54, 218.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (239.81, 125.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.342 CPU sec, 5.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11192, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 133, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (141.05, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (241.49, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.679 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11160, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 139, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (138.40, 53.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.31, 123.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.226 CPU sec, 6.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11169, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 177, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (197.88, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.96, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.494 CPU sec, 5.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11130, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (186.68, 115.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.39, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.397 CPU sec, 5.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11178, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 106, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (147.58, 103.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (233.37, 119.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.753 CPU sec, 5.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11039, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 147, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (150.26, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (236.49, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.329 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11063, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (158.87, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (234.07, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.691 CPU sec, 5.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11049, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 186, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (201.22, 139.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (235.09, 118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.352 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10956, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 134, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (163.18, 108.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.95, 117.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.034 CPU sec, 5.609 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10809, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 196, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (210.83, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (230.13, 116.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.319 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10678, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 116, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (135.80, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (227.92, 115.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.007 CPU sec, 6.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10546, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 199, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (169.86, 92.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (228.78, 116.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.538 CPU sec, 5.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10826, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 175, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (211.05, 152.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.89, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.487 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11191, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 176, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (185.14, 105.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (247.43, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.410 CPU sec, 5.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11056, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (182.22, 111.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (246.73, 128.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.526 CPU sec, 6.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11114, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 169, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (200.69, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (247.03, 128.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.009 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11060, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 121, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (170.67, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 213, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (246.68, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.819 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11058, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 170, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 309) [M::mem_pestat] mean and std.dev: (153.27, 35.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 213, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (245.23, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.977 CPU sec, 5.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11276, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 167, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (187.45, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (243.17, 126.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.509 CPU sec, 5.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11218, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 165, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (169.06, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.05, 127.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.851 CPU sec, 7.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11299, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 202, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (228.12, 101.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (247.01, 127.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.252 CPU sec, 6.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 11139, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 121, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (145.72, 86.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.38, 124.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.982 CPU sec, 5.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11155, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 216, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (174.00, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (242.42, 125.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.115 CPU sec, 6.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11151, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 137, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (129.00, 68.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (244.84, 126.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.754 CPU sec, 6.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11158, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 169, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (176.00, 97.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (246.66, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.885 CPU sec, 6.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11100, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (166.70, 99.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (244.49, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.390 CPU sec, 5.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11136, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (177.04, 104.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (243.16, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.680 CPU sec, 5.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11125, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 175, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (169.72, 96.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.75, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.785 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11189, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 171, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (176.08, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (243.72, 127.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.163 CPU sec, 5.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11117, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 184, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (166.35, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.96, 124.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.666 CPU sec, 5.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11140, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 133, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (145.83, 98.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.95, 123.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.218 CPU sec, 6.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11166, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 136, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (128.24, 83.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (242.29, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.952 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11086, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 142, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (174.29, 131.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.86, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.650 CPU sec, 6.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11204, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 171, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (195.79, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.93, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.495 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11160, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 184, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.71, 68.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.34, 120.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.105 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10976, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 114, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (137.24, 78.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (232.08, 117.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.465 CPU sec, 5.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10961, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 155, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (173.47, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (230.20, 117.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.819 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10917, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 133, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (146.18, 75.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (229.86, 115.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.317 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10848, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 134, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (109.67, 49.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (232.20, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.292 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 10550, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 160, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (187.46, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (227.72, 113.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.751 CPU sec, 5.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10446, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 213, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (217.84, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (233.15, 118.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.759 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10793, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 109, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (103.27, 51.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.22, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.701 CPU sec, 6.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11155, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 133, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (164.95, 109.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (245.45, 127.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.715 CPU sec, 7.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11204, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (204.35, 137.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (244.26, 125.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.972 CPU sec, 5.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11098, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 177, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (181.00, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (247.41, 128.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.831 CPU sec, 7.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11155, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 177, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (172.12, 81.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 214, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (247.05, 126.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.982 CPU sec, 5.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11190, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 187, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (188.67, 109.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (245.07, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.666 CPU sec, 5.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11179, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 153, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (155.86, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (241.52, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.487 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11163, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 180, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (177.64, 78.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (246.60, 127.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.376 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11144, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 104, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (109.95, 64.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (244.01, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.225 CPU sec, 6.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11129, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 82, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (103.13, 65.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.70, 125.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.115 CPU sec, 6.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11049, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 188, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (196.53, 106.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (246.14, 127.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.154 CPU sec, 5.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11178, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 210, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (216.30, 127.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.41, 124.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.083 CPU sec, 5.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11064, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 172, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (217.07, 136.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.95, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.478 CPU sec, 5.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11157, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 123, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 218) [M::mem_pestat] mean and std.dev: (116.67, 31.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (242.16, 124.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.857 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11159, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 123, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (134.90, 78.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (240.84, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.669 CPU sec, 6.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11168, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 211, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (213.12, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (240.80, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.778 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11128, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 144, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (138.91, 70.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (242.59, 126.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.172 CPU sec, 5.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11088, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (188.00, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (240.17, 125.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.598 CPU sec, 5.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11031, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (199.57, 148.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (240.29, 122.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.727 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11107, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (143.28, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.62, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.061 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11285, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 106, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (125.94, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (234.72, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.837 CPU sec, 5.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10839, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 184, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (182.06, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (234.42, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.619 CPU sec, 5.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11078, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 163, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (165.33, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (233.60, 119.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.271 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10942, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 235, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (219.50, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (231.64, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.497 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10879, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (149.91, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (231.25, 117.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.083 CPU sec, 5.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10862, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 114, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (131.28, 70.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (228.97, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.791 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 10597, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (169.25, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (229.92, 114.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.878 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10593, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 188, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (189.63, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (227.61, 113.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.648 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10498, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 127, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (146.29, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (231.54, 116.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.279 CPU sec, 5.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11110, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 144, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (154.00, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (240.75, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.268 CPU sec, 6.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11012, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 220, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (198.39, 108.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.30, 121.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.605 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11084, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 214, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (225.67, 136.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.19, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.804 CPU sec, 6.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11031, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 192, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (183.54, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.38, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.852 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11050, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 84, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (125.71, 91.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.70, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.375 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11148, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 88, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (107.86, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.27, 124.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.415 CPU sec, 6.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10977, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 299, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (262.09, 193.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (240.54, 123.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.161 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10924, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 181, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (210.50, 107.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.92, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.579 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11048, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 146, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (171.28, 135.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.05, 124.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.162 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10909, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 155, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (177.94, 114.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.91, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.546 CPU sec, 6.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10976, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 147, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (160.57, 108.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.67, 123.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.354 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11070, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 140, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (167.47, 103.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.65, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.403 CPU sec, 6.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10924, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 157, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (186.90, 100.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.99, 124.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.666 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10974, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 141, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (172.26, 100.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (238.15, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.834 CPU sec, 6.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11043, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (155.40, 101.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.08, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.804 CPU sec, 6.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11008, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (176.11, 83.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (238.54, 122.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.853 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11119, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 164, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (164.85, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.48, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.507 CPU sec, 7.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11117, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 153, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (173.71, 108.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (236.73, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.651 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11076, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (172.44, 145.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (237.10, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.849 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10917, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 152, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (153.47, 64.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (235.44, 119.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.448 CPU sec, 5.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11016, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 156, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (168.53, 93.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (234.45, 119.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.553 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11076, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (131.38, 76.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.20, 117.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.187 CPU sec, 5.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11023, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (116.12, 75.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (230.48, 117.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.586 CPU sec, 5.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 10819, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 164, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (168.19, 68.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (228.44, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.228 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10921, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 169, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (156.83, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.45, 115.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.015 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10807, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 116, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (168.52, 114.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (227.94, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.515 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10794, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 130, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (167.00, 117.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (227.71, 114.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.127 CPU sec, 5.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10473, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 121, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (110.64, 49.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (225.32, 111.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.045 CPU sec, 5.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10677, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 138, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (128.22, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (228.83, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.085 CPU sec, 5.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11136, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 106, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (109.00, 59.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (242.73, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.741 CPU sec, 6.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11102, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 154, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (182.12, 72.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (242.90, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.852 CPU sec, 5.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11086, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (161.23, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.02, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.333 CPU sec, 5.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11113, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 124, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (138.15, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (241.55, 123.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.769 CPU sec, 6.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11159, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 101, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (111.46, 54.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (243.04, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.768 CPU sec, 5.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11226, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 200, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (203.64, 103.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (243.63, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.082 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11160, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (171.74, 97.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (243.02, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.266 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10981, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (166.16, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (241.91, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.497 CPU sec, 6.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11049, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (152.86, 99.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.02, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.166 CPU sec, 6.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11217, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 139, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (159.43, 114.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.37, 126.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.221 CPU sec, 6.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11149, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 176, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (163.60, 111.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.41, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.321 CPU sec, 6.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11219, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 167, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (184.50, 128.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (244.25, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.563 CPU sec, 6.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11243, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 158, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (171.81, 105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (242.68, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.888 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11170, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 199, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (186.88, 76.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.74, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.557 CPU sec, 7.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11184, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (166.38, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (241.16, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.228 CPU sec, 6.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11094, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 128, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (126.33, 42.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.84, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.514 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10976, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (145.71, 77.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (241.28, 125.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.621 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11181, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 112, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (146.88, 102.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (237.21, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.661 CPU sec, 5.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11000, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 105, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (121.88, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (237.09, 121.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.742 CPU sec, 6.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11149, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 182, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (180.30, 109.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (237.06, 121.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.694 CPU sec, 5.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11247, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 161, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (158.47, 85.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.29, 119.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.020 CPU sec, 5.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11131, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 218, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (209.68, 89.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (234.15, 118.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.121 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11071, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 175, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (193.47, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.28, 117.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.474 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10906, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (173.25, 121.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (230.15, 115.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.001 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10734, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 193, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (193.65, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (226.13, 113.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.553 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10886, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 115, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (160.11, 115.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (224.61, 112.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.198 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10531, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (156.29, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (222.25, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.245 CPU sec, 5.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10288, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (148.30, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (224.93, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.057 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10880, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (154.07, 77.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.43, 125.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.276 CPU sec, 5.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10956, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (141.27, 96.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (245.49, 126.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.856 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11061, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 180, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (164.21, 81.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (243.32, 125.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.031 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11027, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 175, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (210.00, 124.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (243.56, 125.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.330 CPU sec, 6.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10955, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 199, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (221.45, 162.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (241.33, 125.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.939 CPU sec, 7.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 10918, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 161, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (175.20, 113.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.26, 124.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.897 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11021, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (158.41, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (241.38, 124.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.325 CPU sec, 6.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10903, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 149, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 298) [M::mem_pestat] mean and std.dev: (146.79, 56.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (240.19, 124.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.808 CPU sec, 6.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11111, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 152, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (147.41, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.36, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.059 CPU sec, 5.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11172, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 156, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (170.67, 96.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.15, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.645 CPU sec, 5.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11093, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (128.62, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (241.12, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.567 CPU sec, 6.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11152, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 122, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 238) [M::mem_pestat] mean and std.dev: (109.36, 25.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (241.98, 124.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.032 CPU sec, 6.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11102, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 141, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (178.38, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.52, 124.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.411 CPU sec, 6.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11193, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (169.38, 109.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (242.41, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.446 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11010, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 127, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (163.96, 113.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.41, 123.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.367 CPU sec, 6.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11121, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 153, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (168.94, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.04, 124.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.870 CPU sec, 6.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11002, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 161, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (188.36, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (239.78, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.735 CPU sec, 6.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11029, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 144, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (170.54, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.32, 121.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.782 CPU sec, 5.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11004, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 80, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (93.79, 51.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (237.56, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.779 CPU sec, 6.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11000, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (150.08, 74.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (234.37, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.015 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10998, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 104, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (124.89, 97.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.99, 118.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.271 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11224, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 155, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (174.83, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (230.43, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.393 CPU sec, 5.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11009, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 194, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (189.47, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (232.86, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.962 CPU sec, 6.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11096, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 143, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (162.75, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (231.27, 116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.035 CPU sec, 5.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10868, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (167.48, 104.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (226.77, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.089 CPU sec, 5.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 10935, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 131, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (138.05, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.71, 112.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.733 CPU sec, 5.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10512, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (159.74, 84.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (223.62, 111.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.199 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10451, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 194, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (235.00, 182.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (221.66, 108.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.991 CPU sec, 5.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10657, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 110, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (161.59, 119.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (231.17, 116.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.522 CPU sec, 6.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11215, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 132, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (192.22, 131.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (235.75, 120.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.002 CPU sec, 5.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11093, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 165, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (158.76, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (233.89, 120.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.000 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11136, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 170, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (182.30, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.99, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.890 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11214, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 209, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (201.61, 116.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (233.63, 119.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.813 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11189, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 187, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (185.61, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.90, 120.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.407 CPU sec, 5.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11310, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 172, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (185.46, 106.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.09, 117.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.071 CPU sec, 5.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11195, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 153, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (159.78, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (232.81, 118.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.671 CPU sec, 5.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11308, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 135, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (169.95, 116.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (235.20, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.114 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11215, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 144, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (180.77, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (241.64, 123.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.682 CPU sec, 6.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11059, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 174, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (177.64, 123.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (242.68, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.912 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11099, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 129, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (132.68, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.97, 123.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.285 CPU sec, 6.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11173, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 177, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (182.44, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.66, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.758 CPU sec, 6.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11234, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 114, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (195.24, 155.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.12, 123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.468 CPU sec, 5.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11036, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (181.13, 114.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (241.64, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.487 CPU sec, 6.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11108, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 215, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (187.52, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.31, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.738 CPU sec, 6.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11314, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (164.70, 102.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.35, 122.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.118 CPU sec, 5.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11025, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 94, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (146.94, 122.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (240.94, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.163 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11012, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 106, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (133.29, 99.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.96, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.588 CPU sec, 5.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10855, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 107, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (124.95, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.95, 122.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.627 CPU sec, 6.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10903, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 174, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (175.20, 107.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (237.14, 121.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.214 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10854, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 185, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (171.67, 88.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.89, 119.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.655 CPU sec, 6.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11006, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 163, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (168.65, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.21, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.543 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11191, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 146, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (154.05, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (238.98, 122.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.511 CPU sec, 6.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11196, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (192.27, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (240.81, 124.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.964 CPU sec, 6.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11056, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (165.31, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (241.62, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.524 CPU sec, 5.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11149, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 221, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (202.29, 116.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.67, 127.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.495 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11131, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (188.74, 106.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.50, 124.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.200 CPU sec, 6.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11369, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 150, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (196.11, 92.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (237.13, 121.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.018 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11199, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (166.30, 101.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (236.00, 120.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.936 CPU sec, 5.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11278, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (151.83, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.01, 120.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.003 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11128, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 124, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (141.59, 78.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.89, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.165 CPU sec, 5.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11131, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 177, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (190.03, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (230.68, 116.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.897 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11251, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 181, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (199.87, 135.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (234.43, 119.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.534 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11088, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (140.59, 100.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (233.99, 118.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.582 CPU sec, 5.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11194, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 231, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (222.50, 101.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (231.97, 116.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.487 CPU sec, 5.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11237, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 145, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (158.06, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (236.36, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.982 CPU sec, 5.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11217, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 173, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (155.40, 59.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (241.66, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.737 CPU sec, 6.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11180, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 274, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (243.30, 177.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (245.10, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.147 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11166, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 192, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (171.60, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.96, 123.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.321 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11280, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (103.41, 46.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.17, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.572 CPU sec, 5.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11104, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 153, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (155.22, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (240.52, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.534 CPU sec, 6.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11061, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 92, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (117.62, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (242.84, 125.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.222 CPU sec, 7.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11204, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 167, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (165.59, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.52, 123.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.141 CPU sec, 5.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11256, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 111, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (126.91, 53.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.19, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.489 CPU sec, 5.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11172, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 177, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (188.00, 117.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.05, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.637 CPU sec, 6.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11229, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 145, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (168.12, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.75, 122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.751 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10983, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 139, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (174.61, 128.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (241.61, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.259 CPU sec, 5.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 10899, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (152.30, 100.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.91, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.302 CPU sec, 7.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11027, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (127.00, 58.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (238.45, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.393 CPU sec, 5.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11015, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 129, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (164.65, 96.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.35, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.704 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11056, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 125, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (139.72, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (238.22, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.520 CPU sec, 5.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11207, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 183, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (201.38, 107.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (240.82, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.739 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11210, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 155, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (191.65, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (240.50, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.186 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11215, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 210, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (188.62, 111.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (239.95, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.618 CPU sec, 6.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10983, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (178.57, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (242.61, 125.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.845 CPU sec, 7.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11306, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 161, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (132.00, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.40, 122.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.595 CPU sec, 5.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11246, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 201, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (199.58, 109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (231.31, 117.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.301 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11121, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 134, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (162.06, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (236.93, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.428 CPU sec, 5.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11174, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 148, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (140.41, 88.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.02, 117.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.243 CPU sec, 5.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11145, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 165, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (198.04, 127.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (230.79, 116.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.211 CPU sec, 6.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11004, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 167, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (146.50, 85.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (233.81, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.146 CPU sec, 5.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10990, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 152, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (149.00, 81.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (229.58, 115.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.547 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11174, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 158, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (171.50, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (228.63, 114.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.846 CPU sec, 5.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11139, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 150, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (117.00, 78.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.53, 122.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.096 CPU sec, 5.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11252, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 176, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (170.72, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.56, 125.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.248 CPU sec, 6.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11209, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (143.53, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (243.09, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.624 CPU sec, 5.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11061, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 156, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (169.04, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.22, 122.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.258 CPU sec, 5.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11171, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 136, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (169.78, 146.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.63, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.312 CPU sec, 5.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11064, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (166.43, 113.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (240.73, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.388 CPU sec, 5.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11219, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 105, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (147.05, 100.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.21, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.612 CPU sec, 6.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11191, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 135, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (166.07, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.14, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.829 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11343, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 123, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (154.77, 86.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (237.78, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.622 CPU sec, 6.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11267, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 171, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (206.86, 138.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.68, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.915 CPU sec, 5.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10823, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 121, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (125.25, 64.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.10, 121.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.273 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11025, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 120, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (167.71, 156.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.57, 121.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.241 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11060, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 178, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (177.33, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.88, 121.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.524 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11154, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (139.81, 82.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.16, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.187 CPU sec, 6.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11153, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 116, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (148.35, 89.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (242.60, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.876 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11208, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 215, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (215.83, 169.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (240.13, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.855 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11085, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (192.48, 130.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.95, 125.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.531 CPU sec, 5.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11100, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 112, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (137.40, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (242.81, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.083 CPU sec, 5.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11163, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 219, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (242.90, 125.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (241.69, 124.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.229 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11249, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 178, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (166.13, 109.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (241.89, 125.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.830 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 10886, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 160, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (189.87, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (230.45, 114.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.352 CPU sec, 6.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11064, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 137, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (158.19, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (232.40, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.841 CPU sec, 6.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11122, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 170, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (202.75, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (231.01, 117.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.612 CPU sec, 6.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11085, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (122.25, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.12, 117.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.322 CPU sec, 7.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11093, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 172, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (176.55, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (232.90, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.140 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10940, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 104, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (128.46, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.67, 117.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.628 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11110, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 171, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (155.40, 92.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.72, 117.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.140 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10991, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 137, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (151.39, 82.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (231.67, 117.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.910 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11035, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 109, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (140.68, 100.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.82, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.888 CPU sec, 6.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11059, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 129, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (178.85, 124.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.89, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.783 CPU sec, 6.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11173, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 148, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (174.88, 115.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.10, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.499 CPU sec, 6.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10962, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 125, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (147.95, 87.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.07, 121.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.262 CPU sec, 6.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11177, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (145.50, 83.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (238.89, 123.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.479 CPU sec, 6.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11233, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 112, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (135.15, 101.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.87, 122.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.634 CPU sec, 6.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11123, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 149, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (145.73, 83.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.75, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.881 CPU sec, 6.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10917, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 221, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (208.50, 112.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.31, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.612 CPU sec, 6.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11125, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (127.58, 75.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.20, 119.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.805 CPU sec, 6.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11087, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 181, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (180.33, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (233.09, 118.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.987 CPU sec, 6.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10832, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 197, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (206.06, 156.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (228.81, 115.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.291 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10853, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 188, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (178.91, 86.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (226.34, 113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.863 CPU sec, 6.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10870, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 106, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (165.75, 157.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.22, 118.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.475 CPU sec, 6.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11039, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (159.53, 107.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (232.80, 118.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.427 CPU sec, 6.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10771, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 155, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (208.10, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.15, 121.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.867 CPU sec, 6.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11165, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 166, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (167.53, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.39, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.880 CPU sec, 6.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11075, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 167, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (148.31, 96.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (238.02, 121.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.999 CPU sec, 7.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11076, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 105, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (123.53, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.50, 123.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.183 CPU sec, 6.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11054, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 159, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (181.68, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (236.24, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.875 CPU sec, 6.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11130, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 121, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (152.00, 120.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.97, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.291 CPU sec, 6.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11114, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 197, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (240.42, 128.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (233.26, 118.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.196 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11056, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 113, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (148.47, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.60, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.547 CPU sec, 5.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11110, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 156, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (202.12, 149.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (231.56, 117.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.059 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11139, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 189, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (173.13, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (229.61, 116.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.954 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11014, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 145, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (144.67, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.83, 117.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.011 CPU sec, 5.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11146, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 123, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 219) [M::mem_pestat] mean and std.dev: (121.26, 33.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (228.47, 114.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.189 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11150, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (205.11, 103.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (227.39, 113.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.919 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11119, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 85, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (115.82, 63.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (233.77, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.390 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11053, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 125, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (156.21, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.45, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.363 CPU sec, 6.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11149, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 137, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (177.58, 141.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (238.27, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.114 CPU sec, 6.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11060, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 176, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (210.64, 139.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (237.08, 120.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.789 CPU sec, 6.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11055, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (110.62, 51.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.67, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.482 CPU sec, 6.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11121, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 168, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (191.45, 116.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.42, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.868 CPU sec, 6.626 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11124, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (137.11, 65.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (234.22, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.404 CPU sec, 6.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11172, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 134, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (153.60, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (237.35, 121.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.844 CPU sec, 6.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11101, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (133.44, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (234.65, 119.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.476 CPU sec, 6.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11083, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (199.32, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.59, 120.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.208 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10923, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 215, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (185.92, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (229.40, 116.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.053 CPU sec, 5.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10616, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 232, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (233.50, 152.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.67, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.954 CPU sec, 6.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10846, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 153, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (148.95, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (230.81, 115.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.694 CPU sec, 5.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10946, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (150.53, 99.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (230.34, 117.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.091 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10998, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (141.43, 54.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.15, 119.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.758 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11044, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (133.92, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.76, 120.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.202 CPU sec, 5.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10972, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 158, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (181.86, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.45, 121.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.612 CPU sec, 6.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11025, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 143, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (161.64, 94.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (236.32, 120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.579 CPU sec, 6.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11208, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (205.79, 109.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.90, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.670 CPU sec, 6.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11246, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 142, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (164.33, 108.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (238.35, 122.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.747 CPU sec, 5.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11313, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 142, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (142.95, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.40, 119.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.148 CPU sec, 5.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11067, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 167, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (171.70, 82.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.41, 116.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.740 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11130, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 99, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (125.05, 80.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.87, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.899 CPU sec, 5.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11031, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 235, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (204.15, 96.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (228.06, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.296 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11029, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 216, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (199.75, 132.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (228.66, 116.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.056 CPU sec, 5.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11059, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 171, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (155.55, 77.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.12, 115.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.850 CPU sec, 5.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11130, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 114, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (148.00, 81.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (228.40, 115.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.577 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10815, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 136, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (161.10, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (228.54, 115.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.294 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11086, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 104, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (118.58, 42.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.25, 120.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.619 CPU sec, 5.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11097, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 126, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (150.63, 84.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.71, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.110 CPU sec, 6.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11140, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 185, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (169.55, 100.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (236.49, 120.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.738 CPU sec, 6.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11024, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 139, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (145.35, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.74, 121.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.668 CPU sec, 7.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11147, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 161, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (196.22, 116.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.60, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.964 CPU sec, 5.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11198, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 162, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (177.12, 104.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.45, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.024 CPU sec, 6.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11246, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 146, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (140.30, 53.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (235.99, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.048 CPU sec, 5.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11150, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 226, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (219.57, 107.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.90, 117.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.020 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10933, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 179, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (245.68, 168.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (232.13, 117.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.578 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10931, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (127.07, 66.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (232.43, 118.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.786 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10800, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (148.53, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (234.34, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.549 CPU sec, 6.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10964, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 135, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (151.56, 75.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.41, 117.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.378 CPU sec, 6.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11013, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (147.10, 90.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (232.00, 118.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.590 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10988, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (209.00, 162.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (233.05, 118.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.542 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11029, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 175, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (167.38, 72.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (234.24, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.496 CPU sec, 5.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11235, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 171, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (180.11, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.90, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.001 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11136, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (176.94, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.60, 120.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.792 CPU sec, 6.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11076, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (133.11, 102.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.39, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.770 CPU sec, 7.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11060, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 145, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (133.78, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.93, 121.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.935 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11124, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 227, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (218.15, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.07, 121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.861 CPU sec, 6.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11096, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 140, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (184.22, 120.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (234.43, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.662 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11081, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (161.20, 113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.96, 116.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.463 CPU sec, 6.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10982, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 135, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (144.30, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (229.70, 117.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.134 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10841, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 93, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (161.75, 124.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (231.16, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.022 CPU sec, 5.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10913, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 131, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (141.75, 78.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (226.87, 113.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.074 CPU sec, 5.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10842, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 138, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (151.83, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (227.99, 114.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.157 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10714, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 195, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (173.08, 68.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.74, 112.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.665 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 10530, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 178, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (185.96, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (230.11, 115.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.044 CPU sec, 5.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10977, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 227, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (211.17, 95.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (238.15, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.908 CPU sec, 5.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11012, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 122, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (134.13, 94.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (240.23, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.848 CPU sec, 5.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11280, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (175.29, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.72, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.761 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11092, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 174, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (151.71, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.54, 124.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.172 CPU sec, 5.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11005, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (154.06, 108.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (237.69, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.475 CPU sec, 5.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11138, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 157, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (184.78, 113.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.15, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.680 CPU sec, 6.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11139, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 106, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 216) [M::mem_pestat] mean and std.dev: (105.83, 31.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (238.12, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.227 CPU sec, 5.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11178, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 122, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (175.17, 126.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.92, 122.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.399 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11241, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 156, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (170.40, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (236.57, 120.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.402 CPU sec, 5.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11183, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 157, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (161.16, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.63, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.438 CPU sec, 6.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11246, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (154.87, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.83, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.681 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11067, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 157, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (175.19, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.29, 125.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.644 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11080, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 158, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (158.55, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (241.30, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.886 CPU sec, 5.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11065, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 101, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (144.75, 129.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (242.73, 123.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.734 CPU sec, 5.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11104, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 134, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (116.41, 33.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (241.08, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.381 CPU sec, 5.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11095, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 162, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (187.36, 109.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.06, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.603 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11055, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 139, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (172.82, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (242.04, 124.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.148 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11115, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (159.29, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (240.84, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.939 CPU sec, 6.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11210, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.25, 121.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.685 CPU sec, 5.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11190, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 132, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (164.89, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.90, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.159 CPU sec, 5.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11099, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 135, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (141.52, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (237.22, 120.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.617 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11140, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 150, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (165.86, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.00, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.738 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10956, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 152, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (147.29, 78.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (232.14, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.999 CPU sec, 6.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11037, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 141, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (164.12, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (230.17, 116.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.606 CPU sec, 5.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10926, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (169.44, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (227.04, 113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.827 CPU sec, 6.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10661, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 185, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (165.54, 80.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (228.34, 113.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.167 CPU sec, 6.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10541, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 189, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (185.18, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (226.40, 113.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.709 CPU sec, 5.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10459, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 163, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (154.27, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (233.92, 117.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.941 CPU sec, 5.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11098, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 161, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (156.06, 100.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.69, 122.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.030 CPU sec, 5.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11092, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 175, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (205.14, 110.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.90, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.194 CPU sec, 5.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11131, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 166, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (169.53, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (244.93, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.110 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11106, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 185, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (185.33, 110.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (243.64, 125.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.995 CPU sec, 5.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11158, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 97, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (127.41, 85.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.16, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.672 CPU sec, 5.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11251, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (220.00, 133.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.34, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.965 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11400, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 166, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (125.83, 61.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.60, 121.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.671 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11337, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 177, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (171.48, 67.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (242.60, 126.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.047 CPU sec, 7.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11250, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 132, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (163.15, 89.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.65, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.077 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11107, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 159, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (170.39, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (237.81, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.394 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11179, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 209, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (177.00, 94.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (245.01, 127.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.216 CPU sec, 5.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11007, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 110, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (162.00, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (245.26, 125.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.338 CPU sec, 6.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11139, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 158, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (203.85, 122.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (245.94, 126.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.207 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11113, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (136.19, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (241.64, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.559 CPU sec, 5.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11286, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.28, 123.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.872 CPU sec, 5.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11251, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 177, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (171.65, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (245.00, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.944 CPU sec, 5.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11176, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 180, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (121.00, 62.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.43, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.603 CPU sec, 6.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11118, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 158, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (136.56, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.15, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.224 CPU sec, 6.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11052, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 143, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (181.56, 117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.57, 124.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.343 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11384, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 130, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (138.95, 70.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.77, 122.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.916 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11110, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (153.20, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.44, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.908 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11057, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 190, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (204.04, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (230.42, 116.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.550 CPU sec, 5.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11085, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 153, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (152.43, 77.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (232.90, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.388 CPU sec, 6.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10963, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 164, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (163.44, 113.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (234.44, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.159 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11047, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 113, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (179.57, 118.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.36, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.983 CPU sec, 5.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10811, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 98, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (112.58, 59.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (229.93, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.423 CPU sec, 5.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 10606, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 103, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (138.24, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (230.94, 115.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.840 CPU sec, 5.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 10588, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 137, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (142.87, 67.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.61, 117.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.285 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11081, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 107, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (127.17, 67.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.83, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.200 CPU sec, 6.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11124, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (224.45, 104.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (242.27, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.694 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11232, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 166, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (197.92, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (244.66, 126.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.121 CPU sec, 5.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11253, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 145, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (181.14, 128.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.79, 125.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.765 CPU sec, 6.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11262, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 139, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (145.92, 89.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (239.70, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.247 CPU sec, 6.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11181, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 242, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (230.81, 166.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (237.99, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.259 CPU sec, 5.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11377, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 166, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (176.15, 107.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (239.01, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.862 CPU sec, 5.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11392, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 135, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (129.14, 74.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (238.73, 123.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.402 CPU sec, 5.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11163, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (241.11, 106.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.47, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.430 CPU sec, 7.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11374, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 156, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (180.93, 115.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.11, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.859 CPU sec, 5.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11027, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 137, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (149.55, 83.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (243.83, 125.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.611 CPU sec, 5.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11136, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (176.64, 134.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (243.64, 126.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.726 CPU sec, 6.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11291, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 216, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (198.67, 106.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.06, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.737 CPU sec, 5.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11128, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 170, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (187.80, 127.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (241.41, 123.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.251 CPU sec, 5.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11147, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 151, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (174.00, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.30, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.580 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11208, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.81, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.653 CPU sec, 6.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11253, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 183, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (202.04, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (241.81, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.911 CPU sec, 6.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11245, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (128.18, 66.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.86, 124.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.488 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11249, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 117, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (123.06, 48.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (244.18, 126.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.128 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11148, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (137.00, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.64, 118.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.468 CPU sec, 5.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11027, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 154, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (156.40, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (230.48, 115.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.130 CPU sec, 5.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11025, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 209, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (212.36, 122.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (226.36, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.023 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10882, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.69, 43.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (228.16, 114.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.999 CPU sec, 6.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 10623, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (161.60, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (225.60, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.562 CPU sec, 5.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 10847, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 147, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (138.93, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (223.99, 110.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.395 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10637, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 163, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (175.33, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (220.63, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.961 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 10410, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 126, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (178.03, 114.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (217.93, 104.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.422 CPU sec, 6.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10646, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (149.79, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (225.76, 112.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.003 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 10926, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 141, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (198.28, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (238.11, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.930 CPU sec, 5.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11149, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 143, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (147.07, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (238.35, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.616 CPU sec, 6.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11012, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 176, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (171.29, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.95, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.162 CPU sec, 5.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11035, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 167, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (175.55, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (234.29, 119.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.738 CPU sec, 5.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11063, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (119.33, 75.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (239.25, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.794 CPU sec, 6.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11096, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 140, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (145.84, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.59, 120.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.989 CPU sec, 6.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11013, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (126.05, 83.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.74, 120.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.779 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11084, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 140, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (216.87, 162.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.74, 119.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.857 CPU sec, 6.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 11148, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (137.33, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (234.14, 119.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.372 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11144, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 139, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (133.24, 76.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (235.83, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.606 CPU sec, 5.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10923, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 139, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (125.47, 43.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (238.51, 121.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.058 CPU sec, 5.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11106, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (166.18, 107.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.08, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.323 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10889, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 234, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (207.44, 103.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.85, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.542 CPU sec, 5.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11044, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 110, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (141.64, 105.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.02, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.509 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11050, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 153, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (179.22, 131.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.50, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.354 CPU sec, 5.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10960, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 147, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.61, 83.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (242.38, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.603 CPU sec, 7.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11071, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 144, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (145.19, 83.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.63, 122.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.964 CPU sec, 6.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11079, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (148.47, 62.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (238.79, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.855 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10970, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 151, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (157.00, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.29, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.497 CPU sec, 6.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11110, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 150, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (140.89, 64.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (229.94, 115.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.885 CPU sec, 5.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11057, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 145, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (185.88, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (229.32, 116.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.659 CPU sec, 5.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11040, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 93, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (107.94, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (227.18, 114.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.107 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10964, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 174, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (142.60, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (224.64, 111.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.136 CPU sec, 5.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10867, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 157, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (167.50, 86.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (225.17, 112.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.835 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10580, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (220.71, 108.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.176 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 10543, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 95, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (102.07, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (222.19, 109.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.152 CPU sec, 6.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10262, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 138, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (133.55, 74.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (220.09, 107.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.216 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10713, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 146, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (191.45, 127.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.09, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.477 CPU sec, 6.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11192, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 139, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (158.05, 94.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (238.24, 122.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.541 CPU sec, 6.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11069, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 144, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (137.89, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.68, 123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.411 CPU sec, 5.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11072, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (176.05, 103.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.38, 121.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.115 CPU sec, 5.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11205, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 134, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (157.50, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (237.58, 121.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.656 CPU sec, 6.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11012, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 100, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (134.47, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (238.10, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.862 CPU sec, 6.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10970, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 142, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (133.79, 65.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.48, 121.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.328 CPU sec, 5.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11239, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (178.67, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.00, 119.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.903 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11166, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 143, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (167.39, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.42, 120.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.126 CPU sec, 5.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11159, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (165.61, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (233.39, 119.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.226 CPU sec, 6.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11152, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 142, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (152.22, 85.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (236.25, 120.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.645 CPU sec, 6.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11114, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 141, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (170.44, 100.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (237.83, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.933 CPU sec, 7.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11166, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (124.96, 65.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.58, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.910 CPU sec, 6.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11066, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 152, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (152.94, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.37, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.228 CPU sec, 5.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11234, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 176, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (159.00, 71.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (238.66, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.955 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11048, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (147.10, 64.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.23, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.130 CPU sec, 5.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11113, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 197, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (174.21, 56.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.31, 124.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.266 CPU sec, 5.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11159, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 186, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (245.92, 209.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.29, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.621 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11291, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 142, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (170.00, 89.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (241.83, 123.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.991 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11232, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (177.10, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.70, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.477 CPU sec, 5.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11140, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (126.00, 61.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (233.43, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.328 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11128, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 178, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (180.71, 88.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (229.33, 115.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.251 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11047, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 163, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (169.78, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (229.61, 115.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.752 CPU sec, 5.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10807, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 107, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (143.12, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.49, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.001 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 10875, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 178, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (173.38, 73.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.41, 111.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.095 CPU sec, 5.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10810, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 137, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (152.44, 93.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (226.82, 114.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.023 CPU sec, 5.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 10661, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 152, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (165.70, 108.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (225.66, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.995 CPU sec, 5.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10561, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 117, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 214) [M::mem_pestat] mean and std.dev: (107.45, 24.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.65, 111.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.027 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10962, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 180, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (189.27, 132.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (233.05, 117.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.599 CPU sec, 6.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11187, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 172, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (151.16, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (237.14, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.001 CPU sec, 6.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11207, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 167, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (162.00, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (238.48, 122.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.514 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11202, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 149, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (181.95, 109.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.81, 123.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.867 CPU sec, 7.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11007, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 125, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (164.31, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (238.94, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.515 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11257, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (158.75, 124.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.80, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.087 CPU sec, 5.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11232, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 198, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (181.82, 83.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.23, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.961 CPU sec, 5.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11258, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 142, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (144.40, 54.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.73, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.859 CPU sec, 6.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11188, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 173, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (190.74, 99.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.73, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.512 CPU sec, 5.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11333, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 159, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (154.33, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.50, 120.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.680 CPU sec, 5.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11052, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 145, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (188.40, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.07, 122.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.370 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11017, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 189, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (200.33, 103.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (239.68, 120.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.468 CPU sec, 5.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11164, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (197.50, 91.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (237.24, 120.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.743 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10945, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (162.08, 104.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.47, 122.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.741 CPU sec, 6.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11214, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (161.00, 67.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (235.06, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.876 CPU sec, 6.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11256, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 183, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (159.67, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.18, 122.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.216 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11174, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (141.75, 54.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.57, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.319 CPU sec, 6.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11127, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 149, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (172.64, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.16, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.414 CPU sec, 5.902 real sec [M::process] read 8830 sequences (1333330 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11259, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (205.95, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (241.74, 124.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.635 CPU sec, 5.897 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 1526, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (233.13, 118.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 8830 reads in 25.463 CPU sec, 1.308 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21152\tSM:UG10S1P011\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21152_HLKNYBGXX_UG10S1P011_R1.fastq.gz 5347_7332_21152_HLKNYBGXX_UG10S1P011_R2.fastq.gz [main] Real time: 4006.160 sec; CPU: 107530.469 sec Finished alignement of UG10S1P011 at Wed Apr 3 00:46:37 2019 Sorting BAM file started at Wed Apr 3 00:46:37 2019 samtools sort -@ 30 -m 4G UG10S1P011_unsorted.bam -o UG10S1P011.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 00:46:37 2019 rm UG10S1P011_unsorted.bam rm 5347_7332_21152_HLKNYBGXX_UG10S1P011_R1.fastq.gz rm 5347_7332_21152_HLKNYBGXX_UG10S1P011_R2.fastq.gz samtools index UG10S1P011.bam Finished BAM file generation of the sample - UG10S1P011 Processing fastq files for the sample - UG10S2P087 Reading 5347_7332_21153_HLKNYBGXX_UG10S2P087_R1.fastq.gz 5347_7332_21153_HLKNYBGXX_UG10S2P087_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21153\tSM:UG10S2P087\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21153_HLKNYBGXX_UG10S2P087_R1.fastq.gz 5347_7332_21153_HLKNYBGXX_UG10S2P087_R2.fastq.gz | samtools view -Sb1 - > UG10S2P087_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12047, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (166.87, 81.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.86, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.988 CPU sec, 6.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12132, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 112, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (131.65, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (237.87, 118.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.868 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12068, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 121, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (128.81, 62.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.48, 118.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.575 CPU sec, 5.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12288, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 143, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (171.16, 111.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (236.36, 118.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.171 CPU sec, 5.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12217, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 143, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (169.82, 119.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (238.51, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.882 CPU sec, 5.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12214, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 201, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (177.47, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.09, 119.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.044 CPU sec, 5.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12264, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 161, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (167.04, 91.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (239.91, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.539 CPU sec, 5.071 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12229, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (126.88, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (238.17, 120.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.156 CPU sec, 5.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12277, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 139, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (167.36, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.90, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.857 CPU sec, 4.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12144, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 214, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (189.00, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (239.36, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.109 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12368, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (136.19, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (238.07, 119.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.721 CPU sec, 5.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12238, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 131, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (159.07, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.74, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.693 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12251, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (146.50, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (241.81, 121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.939 CPU sec, 5.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12166, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 160, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (175.76, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (237.33, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.830 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11950, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 174, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (173.75, 81.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (236.12, 119.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.420 CPU sec, 6.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12215, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 167, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (162.17, 91.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.23, 117.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.434 CPU sec, 5.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12034, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 113, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (159.24, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (232.89, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.537 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12189, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 102, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (120.67, 70.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.95, 117.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.948 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12188, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 115, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (124.45, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.86, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.911 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12295, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 127, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (136.32, 61.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (235.97, 117.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.811 CPU sec, 5.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12267, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 148, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (157.61, 99.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (230.70, 114.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.088 CPU sec, 4.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12336, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 179, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (183.68, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (234.66, 117.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.069 CPU sec, 4.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12351, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (152.35, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (230.87, 115.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.123 CPU sec, 5.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12149, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 216, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (216.25, 116.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (230.01, 116.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.676 CPU sec, 5.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12159, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 138, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (182.89, 118.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (227.85, 112.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.926 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12159, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 158, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (176.10, 107.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (229.08, 115.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.065 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12217, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 148, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (155.58, 78.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.81, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.330 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12135, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 173, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (150.21, 81.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (229.78, 114.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.717 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11845, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 160, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (172.71, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (240.17, 120.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.454 CPU sec, 5.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11946, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 142, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (140.86, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.69, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.072 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11929, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (174.91, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.59, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.585 CPU sec, 7.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11930, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (144.00, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.68, 118.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.125 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12054, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (129.96, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.84, 118.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.560 CPU sec, 6.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12176, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 159, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (147.71, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.83, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.871 CPU sec, 5.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12324, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 108, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (164.50, 116.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (237.87, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.335 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12307, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (141.43, 69.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.60, 118.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.025 CPU sec, 6.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12223, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (215.54, 113.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (238.66, 121.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.668 CPU sec, 5.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12234, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 146, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (182.76, 110.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (237.46, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.429 CPU sec, 5.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12206, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (203.22, 98.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.93, 119.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.752 CPU sec, 6.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12423, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (127.61, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (235.82, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.185 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12409, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (205.75, 106.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (237.37, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.438 CPU sec, 4.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12435, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (130.87, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (237.08, 118.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.128 CPU sec, 5.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12268, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 145, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (176.42, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.46, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.418 CPU sec, 6.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12320, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 145, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (154.63, 78.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (237.42, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.976 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12319, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 123, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (124.57, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (236.73, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.867 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12263, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 137, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (158.75, 107.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (236.46, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.547 CPU sec, 4.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12195, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (149.26, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (234.04, 118.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.575 CPU sec, 6.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12305, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (177.96, 120.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (234.32, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.992 CPU sec, 5.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12444, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 107, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (162.65, 140.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (230.63, 114.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.224 CPU sec, 4.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12270, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 151, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (208.89, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (232.38, 116.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.370 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12234, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (185.27, 125.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (232.39, 115.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.743 CPU sec, 4.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12214, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 136, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (156.88, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (228.16, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.637 CPU sec, 4.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12151, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 104, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (113.72, 62.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.87, 113.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.945 CPU sec, 4.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12142, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 163, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (155.52, 94.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.82, 111.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.474 CPU sec, 5.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12224, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (150.46, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.84, 112.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.973 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12226, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 143, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (146.55, 85.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (227.40, 111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.258 CPU sec, 5.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12092, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (117.11, 65.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.40, 117.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.953 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11878, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 172, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (194.39, 136.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.20, 119.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.934 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12005, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 154, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (169.46, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (237.16, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.575 CPU sec, 5.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12086, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (178.63, 110.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (234.87, 118.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.539 CPU sec, 5.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11996, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (166.88, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.27, 116.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.509 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12052, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 185, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (160.13, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.52, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.300 CPU sec, 5.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11950, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 95, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (162.18, 122.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.76, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.470 CPU sec, 6.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12112, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 98, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 181) [M::mem_pestat] mean and std.dev: (87.36, 28.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (237.84, 119.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.483 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12114, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 139, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (163.16, 102.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.41, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.267 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12149, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 175, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (178.08, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.34, 118.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.343 CPU sec, 6.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12284, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 148, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (165.53, 122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (238.92, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.291 CPU sec, 4.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12162, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (137.61, 74.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (236.75, 118.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.697 CPU sec, 5.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12221, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 157, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (178.94, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (234.90, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.898 CPU sec, 5.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12224, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 88, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (137.52, 103.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (236.10, 118.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.476 CPU sec, 5.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12081, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 136, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (153.67, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (234.96, 117.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.716 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12125, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 156, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (159.18, 82.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (234.13, 116.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.071 CPU sec, 6.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12356, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 129, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (161.50, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (234.36, 117.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.907 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12254, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 147, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (142.15, 56.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (233.55, 116.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.804 CPU sec, 4.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12153, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 139, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (153.50, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (231.72, 116.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.854 CPU sec, 5.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12092, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 136, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (162.61, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.04, 116.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.383 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12301, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 118, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (117.78, 63.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (230.21, 114.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.434 CPU sec, 4.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12286, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 116, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (182.29, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (226.93, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.743 CPU sec, 5.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12165, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 94, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (125.48, 84.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (228.96, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.211 CPU sec, 4.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12040, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 177, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (176.50, 70.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (228.78, 114.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.742 CPU sec, 5.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12190, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 116, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (153.05, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (228.45, 113.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.178 CPU sec, 4.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12130, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 186, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (186.09, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.53, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.027 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12248, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 131, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (165.30, 91.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.91, 113.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.216 CPU sec, 5.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12176, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 115, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (144.83, 96.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (229.43, 114.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.400 CPU sec, 4.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11959, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (192.29, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (228.58, 111.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.874 CPU sec, 5.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11860, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 110, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (115.11, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.81, 111.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.672 CPU sec, 5.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12274, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 138, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (168.33, 91.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (230.36, 114.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.308 CPU sec, 5.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12046, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (132.00, 55.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (230.86, 115.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.234 CPU sec, 5.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12224, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 144, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (157.41, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.81, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.316 CPU sec, 5.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12171, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (170.12, 98.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.59, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.686 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12303, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 118, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 239) [M::mem_pestat] mean and std.dev: (120.05, 49.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.42, 118.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.836 CPU sec, 6.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12136, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 147, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (168.74, 112.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.86, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.238 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12276, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (186.45, 133.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (237.13, 120.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.735 CPU sec, 5.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12234, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (171.19, 119.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (235.05, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.850 CPU sec, 6.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12232, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 216, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (247.21, 233.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (235.22, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.720 CPU sec, 5.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12376, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 145, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (173.37, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.55, 117.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.481 CPU sec, 5.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12422, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (168.95, 78.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.17, 118.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.482 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12230, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (143.50, 59.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.74, 117.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.127 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12268, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 113, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (129.59, 101.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (236.16, 119.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.622 CPU sec, 5.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12115, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (158.00, 93.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.51, 116.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.342 CPU sec, 5.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12218, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 169, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (159.31, 67.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.75, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.844 CPU sec, 5.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12169, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 172, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (172.20, 99.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (232.70, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.772 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12027, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 134, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (149.15, 77.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (230.65, 115.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.216 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12320, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 155, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (155.42, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (229.81, 115.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.552 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12306, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 228, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (183.79, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (228.28, 113.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.242 CPU sec, 5.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12112, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 175, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (184.95, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.95, 113.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.478 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12187, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 159, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (146.94, 86.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (227.19, 113.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.226 CPU sec, 5.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12275, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 108, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (142.36, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (227.73, 113.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.671 CPU sec, 4.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12156, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 94, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (118.92, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (226.96, 113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.107 CPU sec, 4.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12121, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (183.08, 129.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.55, 112.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.622 CPU sec, 5.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12155, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 168, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (179.68, 102.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.07, 110.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.665 CPU sec, 5.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12101, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (183.58, 111.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (228.59, 112.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.710 CPU sec, 5.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11981, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 155, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (171.10, 92.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (226.00, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.702 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11905, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 120, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (132.05, 92.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (229.02, 112.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.199 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11797, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (158.11, 119.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (229.42, 112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.593 CPU sec, 5.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11959, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 131, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (165.56, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.15, 115.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.603 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11935, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 183, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (213.81, 108.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (231.19, 116.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.020 CPU sec, 7.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12263, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 150, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (157.67, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.44, 115.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.196 CPU sec, 5.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12181, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 117, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (144.86, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (234.46, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.745 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12347, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (143.09, 54.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.94, 116.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.623 CPU sec, 6.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12255, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 112, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (106.57, 49.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.45, 117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.873 CPU sec, 6.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12205, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 140, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (190.17, 165.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (232.65, 117.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.004 CPU sec, 5.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12161, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 143, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (162.00, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (235.73, 119.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.533 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12248, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 97, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (116.42, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (234.78, 117.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.401 CPU sec, 6.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12193, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 166, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (179.33, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.61, 116.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.733 CPU sec, 6.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12189, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 119, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (136.60, 81.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (236.19, 118.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.635 CPU sec, 5.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12227, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 128, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (152.24, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.50, 119.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.773 CPU sec, 5.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12334, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (195.90, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (233.49, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.920 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12132, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 136, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (149.29, 83.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (232.25, 116.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.481 CPU sec, 5.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12212, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 214, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (204.75, 89.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (232.57, 116.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.862 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12245, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 90, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (130.81, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (231.11, 114.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.341 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12215, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (124.46, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (232.40, 116.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.588 CPU sec, 5.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12186, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 154, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (159.23, 99.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (228.18, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.798 CPU sec, 6.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12269, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 166, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (168.47, 65.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.44, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.008 CPU sec, 6.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12330, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (133.44, 81.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.38, 112.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.262 CPU sec, 24.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12264, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 154, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (144.44, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.68, 112.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.270 CPU sec, 4.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12037, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 165, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (152.04, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.62, 111.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.021 CPU sec, 6.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12064, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 107, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (167.14, 128.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (225.02, 110.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.557 CPU sec, 4.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12082, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 132, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (136.37, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (227.38, 112.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.014 CPU sec, 5.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12120, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 158, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (158.70, 71.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (226.45, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.197 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12152, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (122.39, 49.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (226.33, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.860 CPU sec, 5.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 11830, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (121.71, 57.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (230.99, 113.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.847 CPU sec, 5.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11861, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 172, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (195.73, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (229.91, 114.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.338 CPU sec, 5.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11911, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 161, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (163.82, 78.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (229.34, 113.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.113 CPU sec, 5.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11946, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 112, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (123.04, 59.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (231.20, 116.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.275 CPU sec, 4.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12140, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 176, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (197.95, 137.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (231.30, 115.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.364 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12136, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 130, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (136.14, 64.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (232.79, 115.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.008 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12141, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 166, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.19, 76.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.36, 116.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.898 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12121, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 180, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (184.15, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.31, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.786 CPU sec, 5.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12081, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 137, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (162.37, 100.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.39, 117.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.054 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12233, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 167, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (172.88, 82.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.51, 115.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.641 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12024, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (170.06, 110.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (232.63, 116.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.256 CPU sec, 5.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12102, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 138, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (166.79, 100.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.72, 115.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.918 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11879, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 134, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (154.68, 100.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (230.99, 114.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.473 CPU sec, 5.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12093, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 167, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (178.47, 105.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (231.86, 115.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.396 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11981, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 96, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (130.14, 100.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.13, 117.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.518 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12121, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 132, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (145.00, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (233.68, 116.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.860 CPU sec, 5.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12113, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 133, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (177.00, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (228.61, 113.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.012 CPU sec, 5.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12270, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 145, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (169.38, 104.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (229.81, 113.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.418 CPU sec, 5.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12087, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (152.76, 87.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (230.21, 113.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.685 CPU sec, 5.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12106, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 172, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (180.14, 109.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (225.61, 111.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.690 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11985, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (166.64, 83.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.60, 111.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.412 CPU sec, 4.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12096, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (131.05, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.45, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.125 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12162, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 88, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (89.33, 28.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (225.45, 110.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.847 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12252, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 163, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (165.62, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.25, 109.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.423 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12287, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (120.41, 69.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (224.79, 111.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.370 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12076, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 95, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (130.82, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.23, 108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.398 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12042, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 153, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (146.62, 90.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.45, 109.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.004 CPU sec, 6.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12077, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 146, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (148.30, 85.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.36, 116.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.158 CPU sec, 6.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12227, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 113, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (124.65, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.92, 117.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.741 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12130, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (161.20, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (237.96, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.586 CPU sec, 5.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12155, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 146, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (158.29, 83.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.92, 119.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.280 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12174, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (179.50, 106.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.76, 118.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.313 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12145, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 128, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (124.38, 45.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (237.42, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.203 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12126, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (147.93, 88.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.04, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.006 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12116, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 146, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (182.30, 103.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 206, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (236.87, 117.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.913 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12082, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 174, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (172.21, 95.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.40, 117.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.854 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12268, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 153, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (191.96, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (235.48, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.757 CPU sec, 5.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12235, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 147, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (161.78, 111.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (237.03, 118.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.664 CPU sec, 5.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12218, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 129, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (157.33, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.57, 118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.863 CPU sec, 5.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12184, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 160, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (177.18, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.35, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.685 CPU sec, 5.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12058, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 132, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (128.20, 55.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.40, 118.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.933 CPU sec, 5.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12071, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 146, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (157.28, 60.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (234.28, 118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.625 CPU sec, 5.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12199, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 130, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (148.63, 78.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (237.57, 120.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.671 CPU sec, 5.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12282, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 150, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (162.17, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (235.30, 119.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.999 CPU sec, 4.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12086, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 150, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (149.72, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (232.93, 117.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.436 CPU sec, 5.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12224, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 168, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (179.79, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.44, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.206 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12348, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 173, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (168.18, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (235.70, 117.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.257 CPU sec, 5.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12209, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 171, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (167.21, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (229.78, 114.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.682 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12283, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (163.50, 113.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.45, 114.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.109 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12154, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 141, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (132.62, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (229.44, 113.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.480 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12188, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 109, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (123.15, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (226.65, 111.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.678 CPU sec, 5.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12068, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 143, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (161.80, 85.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (229.85, 113.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.562 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11758, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (162.96, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (224.17, 108.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.170 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11874, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 125, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (141.04, 76.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.70, 110.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.074 CPU sec, 5.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11779, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (149.81, 60.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (225.87, 110.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.553 CPU sec, 5.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11368, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (147.50, 92.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.70, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.008 CPU sec, 6.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12127, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 117, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (121.85, 56.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (241.61, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.102 CPU sec, 5.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12193, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (175.54, 71.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (241.72, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.360 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12192, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 196, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (185.14, 108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (241.17, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.371 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12189, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (136.75, 84.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (240.82, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.200 CPU sec, 5.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12341, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 174, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (140.67, 67.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.69, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.113 CPU sec, 6.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12221, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 128, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (127.65, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (241.78, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.164 CPU sec, 5.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12239, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 167, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (177.85, 119.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (239.13, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.720 CPU sec, 5.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12308, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 143, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (150.52, 88.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (240.50, 121.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.664 CPU sec, 6.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12097, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 143, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (147.79, 103.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (239.08, 121.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.151 CPU sec, 6.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12252, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 132, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (151.69, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (238.88, 120.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.427 CPU sec, 5.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12350, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 122, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (143.39, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.33, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.406 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12295, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 149, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (182.36, 110.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (238.45, 119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.515 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12333, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 111, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (114.33, 47.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (238.66, 120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.377 CPU sec, 6.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12246, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (162.41, 105.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (240.31, 121.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.672 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12317, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 163, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (188.32, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (238.59, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.765 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12190, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 149, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (146.72, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (237.77, 119.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.387 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12389, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 157, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (165.12, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (235.85, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.550 CPU sec, 5.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12142, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (189.18, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.25, 119.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.534 CPU sec, 5.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12290, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 153, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (152.85, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.57, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.872 CPU sec, 5.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12290, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (145.25, 94.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.58, 119.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.868 CPU sec, 5.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12256, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 156, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (156.00, 95.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (232.03, 115.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.845 CPU sec, 4.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12384, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 129, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (119.12, 69.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (229.57, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.303 CPU sec, 5.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11974, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (149.79, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (227.22, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.175 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12079, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 159, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (138.09, 80.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (226.93, 111.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.584 CPU sec, 5.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11896, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (155.36, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.62, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.926 CPU sec, 5.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11925, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (124.03, 69.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.86, 110.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.966 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11553, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 127, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (159.86, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.06, 108.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.447 CPU sec, 6.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11514, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 203, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (193.07, 112.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (229.58, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.157 CPU sec, 6.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11758, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (168.50, 110.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (234.49, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.016 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12209, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 120, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (108.31, 50.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (241.51, 121.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.386 CPU sec, 5.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12118, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 175, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (169.08, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.84, 120.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.348 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11973, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 172, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (176.06, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 208, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (240.69, 122.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.423 CPU sec, 5.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12235, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 135, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (168.70, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.48, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.951 CPU sec, 5.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12154, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 125, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (146.47, 61.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (238.54, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.303 CPU sec, 5.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12049, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (156.87, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (238.87, 120.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.813 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12010, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 115, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (160.00, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.32, 120.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.591 CPU sec, 6.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12200, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 152, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (156.38, 79.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (241.09, 121.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.717 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12193, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (100.41, 47.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (238.63, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.530 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12053, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (130.42, 60.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (239.01, 121.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.657 CPU sec, 5.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12316, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 115, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (134.73, 86.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (236.71, 118.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.885 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12218, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 117, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (149.00, 89.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (238.62, 120.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.957 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12013, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 133, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (174.40, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.86, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.590 CPU sec, 5.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12273, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 168, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (187.68, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (235.48, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.296 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12081, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 193, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (199.07, 88.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.96, 117.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.079 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12457, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 125, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (141.94, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.96, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.058 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12279, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 203, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (195.56, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (237.71, 120.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.528 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12411, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (112.23, 57.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.98, 117.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.991 CPU sec, 5.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12234, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 165, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (197.78, 131.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (231.44, 115.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.857 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12312, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 113, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (141.80, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (230.60, 114.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.709 CPU sec, 6.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12191, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 159, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (166.40, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.17, 112.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.868 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12178, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 116, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (157.90, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (228.74, 112.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.952 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11939, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 113, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (149.14, 82.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (226.37, 111.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.866 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11973, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 108, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (135.25, 85.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (225.08, 110.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.603 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11761, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 130, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (163.54, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (222.61, 108.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.404 CPU sec, 5.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11713, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 135, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (151.19, 58.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (224.12, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.297 CPU sec, 5.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11397, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 178, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (192.05, 120.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (224.13, 108.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.624 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11651, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (114.15, 66.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (230.03, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.498 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12305, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (164.00, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.84, 116.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.519 CPU sec, 6.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12319, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (171.53, 100.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.00, 116.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.779 CPU sec, 5.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12019, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 139, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (175.73, 117.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (233.83, 117.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.547 CPU sec, 5.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11936, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 108, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (110.80, 66.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (232.62, 115.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.783 CPU sec, 4.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12238, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (146.72, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (234.13, 117.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.535 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12274, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 157, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (154.06, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.71, 117.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.655 CPU sec, 5.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12104, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 172, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (177.07, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (234.86, 118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.901 CPU sec, 5.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12208, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 135, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (138.62, 86.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.24, 118.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.030 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12094, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (156.43, 104.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.75, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.928 CPU sec, 4.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12140, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 91, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (138.75, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (234.55, 117.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.509 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12074, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 155, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (188.65, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (235.06, 117.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.120 CPU sec, 5.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12193, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (183.33, 132.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.07, 118.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.711 CPU sec, 5.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12220, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 131, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (131.85, 74.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.03, 118.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.801 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12143, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (137.33, 81.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (236.12, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.955 CPU sec, 5.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12278, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 116, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (115.87, 56.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (231.99, 116.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.610 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12100, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (124.44, 64.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (231.83, 116.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.610 CPU sec, 5.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12280, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 123, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (138.78, 100.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.67, 115.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.613 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12164, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 113, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (118.75, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.37, 116.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.224 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12165, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 171, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (190.36, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (231.65, 115.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.966 CPU sec, 5.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12158, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (124.46, 63.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (227.01, 111.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.928 CPU sec, 5.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12042, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 129, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (122.60, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (228.23, 113.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.680 CPU sec, 4.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12003, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (162.36, 102.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (225.65, 111.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.493 CPU sec, 4.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11843, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 99, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (110.65, 59.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (223.48, 108.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.129 CPU sec, 4.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12175, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 146, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (144.88, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (223.99, 110.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.861 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11983, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 152, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (162.00, 68.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (221.70, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.273 CPU sec, 4.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11759, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 125, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (158.39, 103.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (222.18, 106.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.408 CPU sec, 4.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11770, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (142.53, 61.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (221.85, 108.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.580 CPU sec, 4.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11555, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 148, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (170.86, 76.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (223.26, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.301 CPU sec, 6.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12218, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (130.15, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (239.81, 121.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.490 CPU sec, 5.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12203, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 80, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (90.00, 60.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (235.22, 117.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.044 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12181, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 83, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (103.00, 61.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.98, 116.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.056 CPU sec, 5.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12196, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (151.23, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (237.74, 118.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.037 CPU sec, 5.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12154, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (178.25, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (238.12, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.619 CPU sec, 5.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12114, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 125, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (153.11, 77.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (238.39, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.939 CPU sec, 5.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12120, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 113, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (128.89, 58.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (236.23, 119.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.758 CPU sec, 4.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12226, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (134.04, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (235.77, 117.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.954 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12185, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 78, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (104.20, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 207, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.65, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.895 CPU sec, 5.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12148, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (143.94, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.69, 118.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.907 CPU sec, 6.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12049, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 125, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (141.59, 108.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (237.38, 118.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.864 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12104, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (177.38, 109.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.68, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.344 CPU sec, 5.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12266, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (164.16, 115.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (235.93, 117.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.129 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12234, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 142, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (155.08, 92.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (235.00, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.128 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12281, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (149.82, 99.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (237.95, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.047 CPU sec, 5.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12263, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (168.56, 113.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (234.63, 116.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.979 CPU sec, 4.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12136, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (173.85, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.02, 116.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.362 CPU sec, 5.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12271, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (155.57, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (232.54, 115.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.589 CPU sec, 5.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12110, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (139.90, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (229.82, 114.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.943 CPU sec, 5.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12090, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (160.28, 100.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (230.49, 114.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.496 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12116, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 110, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (139.10, 86.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.67, 111.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.163 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12114, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (131.71, 61.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.73, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.424 CPU sec, 4.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12135, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (169.44, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.03, 110.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.472 CPU sec, 4.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12066, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 144, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (167.67, 103.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (225.26, 110.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.016 CPU sec, 4.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11931, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 212, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (172.27, 107.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (220.82, 107.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.681 CPU sec, 4.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11785, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (126.50, 63.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.56, 106.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.742 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11526, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 120, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (138.09, 83.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.12, 105.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.768 CPU sec, 6.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11342, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 156, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (175.81, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (223.00, 108.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.137 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12144, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 107, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (146.63, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (241.17, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.513 CPU sec, 5.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12094, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 178, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (182.65, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (239.12, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.025 CPU sec, 5.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12291, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 125, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (164.43, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (237.69, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.161 CPU sec, 5.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11966, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (172.95, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (235.51, 117.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.744 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12085, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (151.06, 78.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (237.00, 120.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.601 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12121, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 154, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (181.43, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (239.06, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.972 CPU sec, 5.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12125, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 147, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (174.17, 135.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.45, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.394 CPU sec, 5.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12013, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (108.96, 53.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.16, 118.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.445 CPU sec, 5.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12101, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 122, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (100.77, 60.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (237.76, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.580 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12108, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 80, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (106.15, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (237.14, 119.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.400 CPU sec, 5.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12230, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (116.52, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.15, 118.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.517 CPU sec, 6.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12334, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 160, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (189.45, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.90, 119.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.597 CPU sec, 6.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12012, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 155, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (211.30, 149.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (235.54, 116.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.534 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12112, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 134, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (167.00, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.49, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.638 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12199, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (139.32, 63.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (232.69, 115.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.640 CPU sec, 6.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12280, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 96, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (128.32, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (232.85, 116.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.297 CPU sec, 5.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12149, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 143, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (174.79, 100.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.21, 116.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.837 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12115, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 150, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (152.61, 101.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.29, 118.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.102 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12199, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 100, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (140.86, 114.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (230.58, 114.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.590 CPU sec, 5.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12256, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 161, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (182.65, 115.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.38, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.042 CPU sec, 4.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12173, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (135.79, 69.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (225.33, 110.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.208 CPU sec, 5.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12144, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 184, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (196.05, 101.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.74, 110.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.957 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12049, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (126.65, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (223.21, 109.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.844 CPU sec, 5.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 11925, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 139, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (165.00, 109.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.15, 108.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.013 CPU sec, 5.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11949, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (182.24, 103.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (221.35, 109.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.719 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11714, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (109.58, 53.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (220.09, 106.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.931 CPU sec, 5.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11563, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 138, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (156.97, 72.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 189, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (216.39, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.241 CPU sec, 5.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11635, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 153, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (170.69, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (220.31, 105.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.865 CPU sec, 6.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12116, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (178.55, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (227.76, 112.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.101 CPU sec, 4.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12244, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (132.96, 75.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (227.67, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.364 CPU sec, 5.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12255, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (171.05, 107.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.97, 112.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.287 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12089, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 128, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (156.04, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (224.87, 110.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.409 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12158, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 95, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (119.80, 83.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (226.33, 111.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.761 CPU sec, 4.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12345, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (156.49, 89.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.37, 112.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.648 CPU sec, 4.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12029, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 115, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (168.86, 105.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (226.56, 110.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.895 CPU sec, 5.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12087, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 143, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (169.95, 105.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (227.53, 111.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.503 CPU sec, 4.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12178, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (115.86, 65.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (238.44, 120.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.268 CPU sec, 5.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12026, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 145, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (147.68, 84.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (238.45, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.829 CPU sec, 6.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12050, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 137, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (169.52, 111.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (234.40, 117.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.385 CPU sec, 6.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12156, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 137, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (152.96, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (232.90, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.010 CPU sec, 6.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12289, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (138.55, 92.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.71, 117.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.114 CPU sec, 5.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12076, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 158, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (203.56, 189.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.67, 118.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.286 CPU sec, 4.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12195, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 170, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (145.09, 77.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.42, 116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.167 CPU sec, 5.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12416, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 144, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (161.25, 106.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.67, 117.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.134 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12329, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 192, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (175.76, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.12, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.382 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12016, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 137, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (154.70, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.08, 115.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.781 CPU sec, 6.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11907, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 124, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (156.88, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (234.53, 117.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.328 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12007, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 184, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (180.47, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.40, 116.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.857 CPU sec, 6.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11972, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (119.18, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (232.67, 116.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.646 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12029, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 121, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (147.87, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.56, 115.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.530 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12274, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (154.53, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.35, 116.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.507 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12185, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 175, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (164.22, 94.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (234.77, 118.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.494 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12000, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (107.94, 61.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.37, 118.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.496 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12374, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 165, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (187.56, 115.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (234.82, 118.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.085 CPU sec, 5.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12255, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 133, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (213.81, 169.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (235.22, 117.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.230 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12336, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 167, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (193.42, 110.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.68, 115.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.448 CPU sec, 4.543 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12334, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (152.21, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (229.56, 113.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.299 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12354, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 162, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (179.30, 95.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (229.18, 112.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.778 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12194, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 174, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (186.05, 109.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (227.51, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.129 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12164, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (110.71, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.16, 111.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.622 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12335, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 154, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (159.00, 84.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.71, 111.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.278 CPU sec, 5.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12283, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 117, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 232) [M::mem_pestat] mean and std.dev: (117.55, 46.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.97, 112.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.656 CPU sec, 4.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12079, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 140, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (129.90, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (227.74, 111.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.854 CPU sec, 4.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12252, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 137, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (159.93, 98.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.33, 116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.953 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12395, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 129, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (181.77, 133.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.59, 118.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.040 CPU sec, 5.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12342, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (171.05, 104.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (236.63, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.550 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12289, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 138, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (170.90, 96.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (237.11, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.269 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12251, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 166, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (160.92, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (235.29, 116.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.899 CPU sec, 5.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12206, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 149, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (176.62, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (233.89, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.379 CPU sec, 5.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12352, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 102, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (137.85, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (233.28, 116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.424 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12261, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 130, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (143.89, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (233.15, 115.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.666 CPU sec, 5.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12213, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 189, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (188.19, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (232.07, 116.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.200 CPU sec, 4.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12122, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 138, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (156.45, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (231.07, 113.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.103 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11696, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 211, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (79, 319) [M::mem_pestat] mean and std.dev: (198.17, 36.39) [M::mem_pestat] low and high boundaries for proper pairs: (31, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.74, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.944 CPU sec, 5.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11855, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 131, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (161.00, 100.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (233.67, 115.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.321 CPU sec, 5.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11924, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 83, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (104.29, 48.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.67, 116.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.589 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12038, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 163, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (167.95, 62.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.19, 116.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.155 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12092, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (129.55, 66.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.87, 115.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.785 CPU sec, 6.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12136, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 132, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.95, 49.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.31, 117.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.926 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12102, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 173, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (197.08, 108.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (233.71, 116.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.030 CPU sec, 6.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12211, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 143, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (153.87, 96.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (235.15, 117.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.879 CPU sec, 4.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12191, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 152, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (189.14, 130.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.63, 118.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.345 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12165, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 144, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (163.39, 107.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.91, 116.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.025 CPU sec, 5.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12145, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 171, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (142.36, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.83, 112.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.631 CPU sec, 5.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12329, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 160, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (160.03, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (225.68, 111.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.558 CPU sec, 4.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12054, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 157, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (179.58, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (225.97, 111.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.357 CPU sec, 4.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12101, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (126.00, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.93, 110.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.063 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12161, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 140, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (146.88, 93.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.01, 108.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.425 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12104, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 139, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (165.62, 97.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (224.87, 109.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.510 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12042, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (196.50, 132.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (221.23, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.885 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12260, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 138, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (154.21, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (230.42, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.254 CPU sec, 4.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12191, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 165, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (174.00, 110.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.57, 119.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.630 CPU sec, 4.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12193, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 176, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (187.08, 108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (235.50, 118.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.343 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12321, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 260, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (61, 426) [M::mem_pestat] mean and std.dev: (232.71, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.65, 119.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.968 CPU sec, 5.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12249, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 141, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (159.00, 113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (234.84, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.682 CPU sec, 5.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12301, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (170.83, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.73, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.599 CPU sec, 5.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12185, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 154, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (183.46, 151.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (231.76, 115.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.801 CPU sec, 4.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12308, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (162.21, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.46, 116.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.550 CPU sec, 4.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12324, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 179, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (206.37, 114.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (230.17, 114.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.588 CPU sec, 4.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12210, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 149, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (181.39, 94.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (228.34, 113.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.785 CPU sec, 4.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11808, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (134.04, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.58, 117.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.914 CPU sec, 6.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12032, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (159.12, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.34, 116.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.004 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11904, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 128, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (140.21, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.94, 116.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.149 CPU sec, 5.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12068, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 138, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (155.40, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.67, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.559 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12253, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 160, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (170.20, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (234.39, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.603 CPU sec, 5.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12191, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 107, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (131.63, 65.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (239.13, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.168 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12025, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 169, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (164.88, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (236.95, 120.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.628 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12319, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (131.14, 78.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (235.03, 118.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.606 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12120, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 126, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (140.79, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (236.86, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.134 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12174, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 136, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (156.57, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.85, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.106 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 11835, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 143, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (155.97, 98.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (224.78, 110.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.435 CPU sec, 5.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12362, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 148, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (170.88, 104.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.41, 111.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.966 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12250, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (168.85, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (226.64, 110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.268 CPU sec, 4.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12103, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (172.55, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (223.47, 109.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.362 CPU sec, 4.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12006, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (141.19, 89.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.75, 111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.697 CPU sec, 5.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12059, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 140, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (167.67, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.74, 111.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.924 CPU sec, 5.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12147, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 194, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (172.05, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.34, 107.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.394 CPU sec, 5.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12117, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 149, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (162.90, 99.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (229.72, 115.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.690 CPU sec, 5.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12083, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (154.14, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.33, 117.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.172 CPU sec, 5.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12068, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (151.76, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (232.27, 116.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.041 CPU sec, 6.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12119, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 119, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (143.46, 90.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (233.03, 116.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.339 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12239, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (133.04, 78.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (231.30, 115.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.383 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12059, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (177.71, 122.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (231.73, 116.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.098 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12191, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 147, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (142.38, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (231.16, 116.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.241 CPU sec, 6.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12211, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 119, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (146.91, 100.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (229.16, 113.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.279 CPU sec, 4.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12282, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 149, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (171.65, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (230.67, 115.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.305 CPU sec, 5.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12205, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 213, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (206.00, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (228.61, 114.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.478 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11881, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (164.04, 79.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (228.43, 113.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.317 CPU sec, 5.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11751, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 129, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (150.92, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (226.58, 111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.701 CPU sec, 5.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11888, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 118, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (139.81, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (226.02, 111.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.426 CPU sec, 5.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11926, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 114, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (140.76, 74.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (227.40, 111.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.641 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12077, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 136, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (118.00, 52.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (229.72, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.793 CPU sec, 6.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12265, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 158, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (169.00, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (229.12, 113.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.538 CPU sec, 5.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12286, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 134, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (167.38, 111.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (231.48, 115.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.896 CPU sec, 4.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12199, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (186.36, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (231.09, 114.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.024 CPU sec, 6.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12129, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 177, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (181.72, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (229.92, 114.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.706 CPU sec, 4.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12377, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 135, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (163.87, 99.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (228.25, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.463 CPU sec, 4.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12167, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (141.81, 94.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (227.44, 110.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.850 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12142, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (137.85, 72.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.67, 110.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.033 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12246, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 136, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (171.50, 126.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.49, 111.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.413 CPU sec, 4.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12103, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 174, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (176.75, 93.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (225.27, 111.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.303 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12099, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 99, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (113.61, 53.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (225.43, 109.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.084 CPU sec, 4.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12106, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 108, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (104.58, 62.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (223.66, 108.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.435 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12051, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 136, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (168.10, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.70, 108.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.819 CPU sec, 4.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12223, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 186, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (167.92, 92.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (227.49, 110.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.201 CPU sec, 4.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12170, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 199, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (181.73, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (235.21, 117.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.120 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12242, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (170.52, 117.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (232.20, 116.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.620 CPU sec, 5.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12195, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 92, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (93.13, 43.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (232.74, 117.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.413 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12149, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 131, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (195.16, 128.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.30, 117.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.905 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12193, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 128, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (159.46, 112.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (232.96, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.997 CPU sec, 5.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12109, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 185, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (172.56, 108.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (232.63, 115.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.995 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12164, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 90, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (116.90, 68.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (232.35, 115.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.789 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12091, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 142, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (145.15, 85.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (229.37, 113.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.258 CPU sec, 5.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12213, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 102, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (127.50, 85.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.66, 113.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.904 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11957, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 142, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (162.82, 85.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.54, 112.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.110 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12006, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 142, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (156.72, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.49, 110.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.708 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11799, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (131.90, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (226.00, 110.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.511 CPU sec, 5.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12080, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (120.00, 43.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.59, 111.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.437 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12060, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 151, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (150.76, 81.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (226.97, 111.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.846 CPU sec, 4.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12226, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 133, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (147.97, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (228.37, 112.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.591 CPU sec, 5.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12348, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (140.32, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (231.10, 116.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.354 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12205, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 124, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (167.19, 134.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (229.99, 112.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.917 CPU sec, 5.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12238, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 120, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (139.70, 125.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.67, 116.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.237 CPU sec, 4.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12265, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 114, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (150.81, 110.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (232.31, 119.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.696 CPU sec, 5.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12157, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (152.59, 113.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.37, 109.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.753 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12286, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 158, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (177.11, 105.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.54, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.394 CPU sec, 4.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12084, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 126, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (141.00, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (223.51, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.915 CPU sec, 5.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12074, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (170.10, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (219.99, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.940 CPU sec, 5.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12190, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 181, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (166.22, 90.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.00, 109.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.081 CPU sec, 6.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12045, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 125, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (131.29, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.82, 107.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.617 CPU sec, 5.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11946, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 126, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (120.14, 79.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (223.66, 109.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.894 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12107, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 126, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (113.95, 51.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.33, 105.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.322 CPU sec, 5.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12193, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 123, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (157.57, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (232.09, 116.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.001 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12168, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 145, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (187.13, 122.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (232.28, 116.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.607 CPU sec, 5.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12416, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 131, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (136.33, 92.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (229.82, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.106 CPU sec, 4.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12144, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (187.76, 127.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (231.23, 113.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.662 CPU sec, 6.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12252, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (141.14, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (229.99, 114.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.023 CPU sec, 5.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12227, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 117, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (135.79, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (230.17, 113.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.303 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12027, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 101, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (123.11, 54.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (228.96, 113.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.695 CPU sec, 5.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12139, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 150, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (162.79, 109.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (228.13, 113.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.163 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12122, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 116, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (121.63, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (224.24, 109.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.210 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12156, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 169, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 316) [M::mem_pestat] mean and std.dev: (164.12, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.88, 113.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.309 CPU sec, 5.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11844, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 147, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (147.16, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.05, 112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.908 CPU sec, 5.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12057, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 94, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (121.06, 80.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (228.52, 113.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.168 CPU sec, 5.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11949, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (149.05, 75.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (226.74, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.953 CPU sec, 4.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12009, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (127.26, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (229.92, 113.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.502 CPU sec, 5.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12220, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 94, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (131.78, 85.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (231.43, 115.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.918 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11953, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 118, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (147.00, 94.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (230.75, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.648 CPU sec, 5.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12271, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (164.28, 81.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (231.50, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.831 CPU sec, 5.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12138, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 114, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (137.16, 85.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (235.76, 119.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.033 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12230, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 168, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (205.91, 131.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (231.65, 116.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.520 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12216, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (121.35, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (230.58, 114.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.609 CPU sec, 5.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12122, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 120, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (129.26, 88.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (223.56, 109.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.291 CPU sec, 4.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 11994, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (136.61, 78.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (225.55, 110.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.543 CPU sec, 4.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11993, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (122.00, 60.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (222.59, 106.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.656 CPU sec, 5.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11902, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (172.37, 92.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.65, 106.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.140 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11821, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (158.65, 102.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (224.43, 107.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.451 CPU sec, 5.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11836, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 82, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (128.91, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (222.45, 108.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.889 CPU sec, 5.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11609, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 111, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (163.59, 125.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (226.67, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.123 CPU sec, 6.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11749, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (148.56, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (232.45, 114.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.003 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12308, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (125.50, 64.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.78, 117.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.923 CPU sec, 6.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12357, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (137.14, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.13, 118.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.447 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12124, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 124, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (157.75, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (235.52, 118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.368 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12279, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 118, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (185.86, 147.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.61, 116.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.127 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12326, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 147, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (162.52, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (233.32, 116.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.696 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12176, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (127.18, 70.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (231.50, 115.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.936 CPU sec, 4.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12104, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (131.74, 95.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (232.33, 115.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.042 CPU sec, 4.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12204, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (139.62, 107.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (230.78, 114.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.103 CPU sec, 5.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12319, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (172.00, 112.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (230.35, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.773 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12003, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (140.25, 92.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (236.62, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.105 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12113, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 167, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (197.64, 79.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.20, 116.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.094 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12198, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 132, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (161.50, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (237.46, 118.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.252 CPU sec, 5.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12013, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 143, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (142.72, 62.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (236.35, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.634 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12191, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 128, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (165.53, 99.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.16, 117.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.634 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12159, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 157, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (184.06, 114.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (234.47, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.124 CPU sec, 5.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12218, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 134, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (155.78, 103.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.90, 118.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.441 CPU sec, 5.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12281, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (116.36, 43.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.51, 116.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.668 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12224, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (138.00, 103.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (233.32, 116.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.208 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12280, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (177.35, 110.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (230.83, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.903 CPU sec, 6.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12129, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 162, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (149.35, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (228.73, 113.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.572 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12074, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (144.15, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.34, 109.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.223 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12199, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 87, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (118.11, 79.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.51, 110.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.365 CPU sec, 5.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11919, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 115, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (120.81, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.98, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.419 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11758, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 182, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (176.35, 92.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.55, 108.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.975 CPU sec, 5.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11718, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (134.40, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.37, 108.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.802 CPU sec, 6.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11605, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 139, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (156.00, 93.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.95, 109.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.264 CPU sec, 6.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11397, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 159, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (160.84, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 199, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (230.77, 113.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.179 CPU sec, 5.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12103, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (159.30, 83.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.23, 118.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.307 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12205, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (131.31, 68.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (238.87, 121.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.751 CPU sec, 5.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12224, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (133.43, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (239.54, 119.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.321 CPU sec, 5.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12275, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (174.00, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.71, 118.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.432 CPU sec, 6.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12241, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 205, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (153.79, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (236.20, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.974 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12194, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 143, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (185.68, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.59, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.068 CPU sec, 5.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12401, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 130, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (144.57, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.19, 118.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.616 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12313, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 152, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (165.26, 130.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (230.70, 115.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.326 CPU sec, 5.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12323, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 173, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (190.48, 127.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (230.52, 114.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.886 CPU sec, 5.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12104, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 101, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (142.35, 102.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (235.73, 118.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.562 CPU sec, 5.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12345, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (120.27, 60.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (237.00, 118.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.623 CPU sec, 5.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12117, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 122, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (136.86, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (237.17, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.858 CPU sec, 5.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12206, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 138, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (142.74, 77.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (240.03, 120.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.827 CPU sec, 5.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12260, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 115, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (147.88, 81.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.04, 118.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.762 CPU sec, 5.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12231, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 123, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (144.91, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.41, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.871 CPU sec, 5.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12179, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 149, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (140.96, 63.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (240.59, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.338 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12324, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (145.55, 86.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (236.81, 118.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.461 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12360, 10, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 150, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (135.74, 83.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (237.21, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 1060, 2362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7012) [M::mem_pestat] mean and std.dev: (1577.70, 2078.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9891) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 144.974 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12253, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (172.47, 98.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (237.43, 119.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.462 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12195, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 95, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (114.95, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (228.46, 113.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.240 CPU sec, 5.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12200, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 154, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (163.13, 109.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (227.15, 112.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.981 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11991, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 149, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (143.73, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (228.00, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.075 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12128, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 156, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (223.64, 172.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (226.78, 111.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.279 CPU sec, 4.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12053, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 124, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (152.28, 90.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (226.80, 110.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.819 CPU sec, 5.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11787, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (129.50, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (224.51, 110.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.808 CPU sec, 4.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11868, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (136.76, 77.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (227.29, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.182 CPU sec, 4.994 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11707, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 133, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (185.31, 126.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (227.83, 111.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.444 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11853, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (141.55, 67.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.21, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.510 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12348, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 177, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (155.22, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (238.00, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.852 CPU sec, 6.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12344, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 193, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (209.94, 117.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (234.32, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.582 CPU sec, 5.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12273, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (139.00, 77.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.16, 118.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.148 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12266, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 141, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (147.91, 54.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (234.86, 117.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.921 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12162, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (122.35, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (232.31, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.361 CPU sec, 5.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12197, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 150, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (151.21, 77.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (232.02, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.219 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12296, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 209, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (188.88, 98.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (235.00, 117.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.461 CPU sec, 6.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12407, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 124, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.81, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (234.25, 117.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.112 CPU sec, 4.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12295, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (148.19, 78.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (233.34, 116.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.260 CPU sec, 6.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12081, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 165, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (159.08, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.38, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.821 CPU sec, 5.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12369, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 137, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (166.76, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (237.67, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.867 CPU sec, 5.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12230, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 105, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (130.07, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (234.01, 117.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.212 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12221, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 172, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (179.14, 96.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (234.01, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.069 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12349, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (151.50, 91.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.98, 119.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.147 CPU sec, 6.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12227, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (159.71, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (235.27, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.787 CPU sec, 5.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12182, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 121, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (119.07, 49.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (236.75, 117.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.220 CPU sec, 5.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12252, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 147, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (149.50, 53.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.90, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.380 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12143, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (142.81, 47.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (237.33, 118.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.547 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12168, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 144, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (138.00, 57.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (222.30, 108.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.873 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12127, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 132, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (156.19, 109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.20, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.696 CPU sec, 6.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12189, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 116, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (128.86, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (222.99, 109.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.149 CPU sec, 6.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11938, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 117, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (140.46, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (219.61, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.123 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11802, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 125, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (149.00, 76.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (222.50, 107.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.590 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11809, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (132.15, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (217.97, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.860 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11529, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 162, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (180.00, 110.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (217.62, 104.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.337 CPU sec, 5.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 11414, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 138, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (151.79, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (218.58, 104.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.408 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11930, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 107, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (159.71, 121.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (228.56, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.673 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12096, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 182, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (177.00, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (229.59, 114.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.592 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11996, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (164.88, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (233.02, 116.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.404 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12211, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (153.52, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (234.39, 118.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.046 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12119, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 137, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (158.64, 110.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (229.50, 114.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.757 CPU sec, 5.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12224, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 105, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (129.04, 94.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (231.31, 115.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.342 CPU sec, 4.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12228, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 181, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (154.68, 81.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (228.98, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.311 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12106, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (148.75, 108.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (229.46, 113.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.163 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12188, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (161.95, 110.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (228.11, 113.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.035 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12127, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 156, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (144.41, 75.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (228.87, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.677 CPU sec, 5.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12277, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 174, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (159.05, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (234.49, 116.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.106 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 11985, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 157, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (175.19, 101.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (231.94, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.593 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12172, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 188, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (182.57, 113.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.76, 115.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.557 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12201, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 140, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (147.55, 113.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (231.99, 115.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.172 CPU sec, 6.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12108, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 139, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (180.59, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.25, 116.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.298 CPU sec, 6.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12174, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 95, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (123.19, 69.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.71, 117.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.147 CPU sec, 5.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12103, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 130, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (121.33, 64.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.11, 116.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.698 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12237, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 142, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (143.57, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (233.56, 116.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.722 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12161, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (115.93, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (233.63, 117.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.460 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12291, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 188, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (180.33, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (227.37, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.477 CPU sec, 5.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12180, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 95, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (98.23, 58.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.46, 108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.623 CPU sec, 5.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12081, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 159, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (159.97, 109.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (220.93, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.068 CPU sec, 4.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12040, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 173, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (185.04, 112.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (219.84, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.607 CPU sec, 5.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12051, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (146.97, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (218.16, 104.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.264 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11662, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (156.74, 77.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 189, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (215.77, 102.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.672 CPU sec, 5.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11630, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 155, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (153.19, 86.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 190, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (216.49, 103.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.959 CPU sec, 5.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11511, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (170.19, 72.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 190, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (217.11, 103.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.460 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11738, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 130, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (171.75, 99.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (227.37, 111.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.964 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12352, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.07, 120.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (234.48, 116.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.611 CPU sec, 5.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12445, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (122.43, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (235.25, 118.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.165 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12103, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 150, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (205.22, 169.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (231.84, 115.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.784 CPU sec, 5.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12244, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 97, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (146.75, 103.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (231.29, 115.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.839 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12206, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (151.69, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (232.08, 115.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.120 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12038, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 170, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (159.78, 98.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.53, 116.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.872 CPU sec, 6.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12172, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 103, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (124.92, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (229.07, 113.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.919 CPU sec, 5.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12157, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 162, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (180.25, 118.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (229.19, 114.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.756 CPU sec, 6.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12222, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (107.93, 46.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (225.59, 111.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.979 CPU sec, 5.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12328, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 253) [M::mem_pestat] mean and std.dev: (143.00, 52.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (232.38, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.316 CPU sec, 5.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12132, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 198, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (198.32, 107.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (233.20, 116.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.325 CPU sec, 5.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12202, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 169, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (180.67, 104.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 204, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (235.90, 117.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.366 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12067, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 131, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (139.27, 90.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (232.73, 116.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.192 CPU sec, 5.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12106, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 114, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (134.52, 78.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (230.95, 114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.580 CPU sec, 5.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12152, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (183.56, 114.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (234.79, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.257 CPU sec, 5.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12133, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 130, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (162.80, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.59, 116.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.288 CPU sec, 5.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12221, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 127, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (178.89, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (234.05, 116.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.410 CPU sec, 5.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12070, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 107, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (106.25, 47.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (233.07, 117.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.868 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12092, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (131.00, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (228.95, 112.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.112 CPU sec, 6.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12392, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 157, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (158.32, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.14, 112.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.268 CPU sec, 6.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12151, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (145.31, 78.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (227.06, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.404 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12032, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 184, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (178.76, 111.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (224.38, 108.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.883 CPU sec, 5.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12061, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (163.18, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (225.09, 109.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.865 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11994, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 133, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (139.60, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (220.68, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.232 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11750, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 130, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (154.50, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.12, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.025 CPU sec, 4.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11776, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 126, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (149.41, 92.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.64, 106.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.554 CPU sec, 5.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11780, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 134, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (182.76, 138.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (222.91, 108.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.539 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12207, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 79, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (91.65, 50.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.96, 117.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.621 CPU sec, 6.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12180, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (161.25, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.74, 118.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.296 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12210, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (231.25, 115.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.441 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12166, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 165, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (180.19, 104.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (230.52, 115.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.578 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12129, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 154, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (170.12, 108.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (231.89, 114.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.931 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12210, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 192, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (164.21, 82.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (233.07, 116.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.789 CPU sec, 6.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12263, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 142, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (151.34, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (231.81, 115.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.509 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12148, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 154, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (148.85, 90.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (229.05, 114.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.263 CPU sec, 5.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12401, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 131, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (177.95, 123.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (226.54, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.510 CPU sec, 5.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12111, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 128, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (147.52, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.55, 115.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.350 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12101, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 172, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.07, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.78, 116.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.909 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12233, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 121, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (144.89, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (233.72, 115.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.977 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12187, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (153.25, 107.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (230.74, 115.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.342 CPU sec, 5.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12083, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 130, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (142.48, 74.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (230.25, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.376 CPU sec, 4.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12209, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 121, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (140.72, 70.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (231.86, 115.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.627 CPU sec, 5.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12103, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 165, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (212.32, 128.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (232.44, 116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.915 CPU sec, 5.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12170, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 165, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (180.92, 100.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (234.80, 118.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.148 CPU sec, 5.584 real sec [M::process] read 17250 sequences (2604750 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12094, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 141, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (157.52, 86.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (232.80, 116.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.542 CPU sec, 5.605 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 3167, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (232.87, 116.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 17250 reads in 45.246 CPU sec, 2.017 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21153\tSM:UG10S2P087\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21153_HLKNYBGXX_UG10S2P087_R1.fastq.gz 5347_7332_21153_HLKNYBGXX_UG10S2P087_R2.fastq.gz [main] Real time: 3613.206 sec; CPU: 95102.068 sec Finished alignement of UG10S2P087 at Wed Apr 3 01:56:39 2019 Sorting BAM file started at Wed Apr 3 01:56:39 2019 samtools sort -@ 30 -m 4G UG10S2P087_unsorted.bam -o UG10S2P087.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 01:56:39 2019 rm UG10S2P087_unsorted.bam rm 5347_7332_21153_HLKNYBGXX_UG10S2P087_R1.fastq.gz rm 5347_7332_21153_HLKNYBGXX_UG10S2P087_R2.fastq.gz samtools index UG10S2P087.bam Finished BAM file generation of the sample - UG10S2P087 Processing fastq files for the sample - UG10S2P088 Reading 5347_7332_21154_HLKNYBGXX_UG10S2P088_R1.fastq.gz 5347_7332_21154_HLKNYBGXX_UG10S2P088_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21154\tSM:UG10S2P088\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21154_HLKNYBGXX_UG10S2P088_R1.fastq.gz 5347_7332_21154_HLKNYBGXX_UG10S2P088_R2.fastq.gz | samtools view -Sb1 - > UG10S2P088_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12976, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (151.79, 115.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.44, 71.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.655 CPU sec, 5.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13219, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 101, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (92.33, 28.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (161.14, 72.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.524 CPU sec, 4.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13183, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 72, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (101.88, 59.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.38, 70.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.228 CPU sec, 5.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13255, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 170, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (151.15, 88.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.94, 71.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.593 CPU sec, 6.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13088, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (111.91, 59.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.52, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.136 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13295, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 107, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (120.90, 60.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.77, 71.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.723 CPU sec, 5.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13278, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 75, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (94.06, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.36, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.840 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13139, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 116, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (95.44, 47.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.84, 70.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.228 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13356, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 130, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (133.21, 61.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.50, 70.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.207 CPU sec, 5.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13235, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 168, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (139.07, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.99, 69.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.946 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13263, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 79, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (119.38, 72.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.90, 70.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.483 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13242, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (160.85, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.71, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.585 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13183, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.74, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.784 CPU sec, 5.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13255, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (155.50, 114.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.41, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.172 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13193, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (124.38, 55.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (157.45, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.295 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13088, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 81, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (89.92, 53.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (157.18, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.073 CPU sec, 4.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12965, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.49, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.012 CPU sec, 5.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13049, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (99.50, 52.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.64, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.488 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13162, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 293) [M::mem_pestat] mean and std.dev: (145.30, 32.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.13, 69.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.203 CPU sec, 5.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13085, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 133, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (159.18, 70.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (158.82, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.269 CPU sec, 4.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13236, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 129, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (150.05, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.36, 71.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.997 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13121, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 116, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (118.07, 68.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.93, 71.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.898 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13193, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 90, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (95.61, 52.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.53, 70.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.358 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13242, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 81, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (100.60, 74.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.08, 71.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.025 CPU sec, 5.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13224, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 101, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 164) [M::mem_pestat] mean and std.dev: (84.83, 29.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (157.53, 68.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.900 CPU sec, 5.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13301, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (157.89, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.820 CPU sec, 5.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13201, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 78, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (122.94, 91.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.15, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.490 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13277, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 82, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (117.36, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (157.06, 69.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.109 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13245, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (142.05, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.84, 68.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.408 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13068, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (115.87, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (154.49, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.979 CPU sec, 5.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13310, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 73, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (55.62, 29.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.41, 70.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.296 CPU sec, 4.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13039, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 92, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (130.89, 83.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (156.41, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.079 CPU sec, 4.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13145, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 113, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (184.75, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.23, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.657 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13056, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (133.62, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.31, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.240 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13078, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 146, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (154.75, 106.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.29, 69.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.689 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13081, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 102, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (120.88, 76.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.61, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.117 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13301, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 99, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (105.70, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.07, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.602 CPU sec, 5.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13265, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (104.20, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.69, 71.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.465 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13114, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 113, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (106.83, 59.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.41, 70.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.722 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13204, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (137.00, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (157.57, 69.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.312 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13073, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 85, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (100.88, 49.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.89, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.630 CPU sec, 4.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13244, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 76, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (116.64, 90.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.91, 69.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.640 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13154, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 140, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (140.00, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.49, 69.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.706 CPU sec, 5.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13109, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (125.05, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (155.35, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.302 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13128, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 94, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (117.30, 77.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.26, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.672 CPU sec, 4.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13169, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 170, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (178.20, 80.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.22, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.853 CPU sec, 4.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13074, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 86, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (92.79, 50.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.74, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.531 CPU sec, 6.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12973, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 169, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (180.40, 113.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.41, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.231 CPU sec, 6.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13026, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 100, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 204) [M::mem_pestat] mean and std.dev: (100.31, 20.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (155.97, 67.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.320 CPU sec, 4.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13205, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 80, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (77.83, 45.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.58, 69.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.142 CPU sec, 5.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13275, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (158.31, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.392 CPU sec, 4.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13239, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 93, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (100.86, 44.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.28, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.907 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13035, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.87, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.755 CPU sec, 5.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13091, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (155.53, 86.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.46, 70.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.524 CPU sec, 6.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13327, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 137, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (141.31, 92.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.22, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.028 CPU sec, 5.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13187, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 145, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (161.50, 109.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.23, 70.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.875 CPU sec, 4.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13073, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 121, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (127.50, 67.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.76, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.765 CPU sec, 4.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13234, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (126.07, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (155.97, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.007 CPU sec, 4.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13171, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (117.56, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (158.53, 70.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.125 CPU sec, 6.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13298, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 95, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (107.10, 66.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.73, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.233 CPU sec, 5.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13146, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 91, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (147.12, 117.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (158.13, 70.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.525 CPU sec, 5.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13149, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 136, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (138.58, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.90, 69.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.879 CPU sec, 4.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13226, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 91, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (104.92, 63.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (158.05, 69.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.610 CPU sec, 4.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13027, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 58, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (86.67, 54.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.70, 68.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.198 CPU sec, 5.799 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12979, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 130, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (156.77, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.47, 66.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.285 CPU sec, 5.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13004, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 93, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (149.70, 110.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.31, 68.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.498 CPU sec, 4.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12962, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 112, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (120.36, 57.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.10, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.091 CPU sec, 6.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13221, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 129, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (149.40, 97.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.48, 68.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.436 CPU sec, 5.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13125, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.42, 69.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.307 CPU sec, 5.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13092, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 95, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (127.22, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (157.97, 69.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.001 CPU sec, 6.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13143, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 117, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (156.58, 109.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (157.18, 68.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.561 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13205, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 87, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (72.10, 35.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (155.80, 67.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.981 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13084, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 94, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (102.67, 32.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.38, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.676 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13317, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 114, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (108.07, 48.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (157.36, 69.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.402 CPU sec, 4.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13149, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 151, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 260) [M::mem_pestat] mean and std.dev: (149.36, 34.09) [M::mem_pestat] low and high boundaries for proper pairs: (8, 302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.64, 67.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.353 CPU sec, 4.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13200, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 107, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (134.00, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.44, 69.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.693 CPU sec, 5.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13172, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 105, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (113.50, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (157.83, 70.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.392 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13072, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 100, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (130.21, 71.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.05, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.381 CPU sec, 5.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13341, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 78, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (128.46, 121.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.69, 68.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.772 CPU sec, 5.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12995, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 129, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (137.58, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.38, 68.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.551 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13019, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 150, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (165.47, 96.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.98, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.143 CPU sec, 4.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12997, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 147, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (154.42, 150.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (157.03, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.849 CPU sec, 5.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13230, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 96, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (100.33, 52.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (156.59, 67.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.809 CPU sec, 5.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13109, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.38, 69.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.806 CPU sec, 5.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13105, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 113, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (126.56, 69.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.46, 70.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.857 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13100, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.21, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.956 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13141, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 103, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (93.00, 23.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.13, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.534 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13020, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (196.67, 179.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.05, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.115 CPU sec, 6.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12922, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 84, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (77.36, 29.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.47, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.296 CPU sec, 5.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13183, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 217, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (170.58, 104.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.19, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.305 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13096, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 87, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (128.06, 101.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (157.88, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.099 CPU sec, 4.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13196, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 93, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (142.50, 111.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.78, 69.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.453 CPU sec, 5.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13161, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 89, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (117.35, 92.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.35, 67.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.224 CPU sec, 3.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13209, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (146.92, 75.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (155.23, 66.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.282 CPU sec, 4.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13178, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 130, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (127.64, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.27, 66.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.614 CPU sec, 3.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13241, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 135, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 249) [M::mem_pestat] mean and std.dev: (138.64, 41.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.26, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.935 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13087, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 180, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (172.06, 120.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (160.38, 71.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.603 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13203, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (129.43, 66.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.18, 74.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.533 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13008, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (169.40, 92.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.50, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.708 CPU sec, 4.674 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13225, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 104, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (102.60, 39.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.08, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.971 CPU sec, 4.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13189, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 115, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (110.15, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.63, 71.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.798 CPU sec, 4.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13234, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.37, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.898 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13152, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 98, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (135.09, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (160.50, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.713 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13160, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (130.88, 56.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.24, 70.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.933 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13183, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 132, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (143.93, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.77, 71.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.682 CPU sec, 5.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13160, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (96.25, 53.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.35, 71.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.725 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13090, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (104.40, 38.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (157.64, 70.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.853 CPU sec, 4.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13180, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (105.20, 58.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.02, 68.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.209 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13017, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 108, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (122.69, 67.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.87, 67.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.249 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13172, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 85, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (135.20, 109.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (155.02, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.884 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12986, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (154.72, 66.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.211 CPU sec, 5.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12920, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.58, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.785 CPU sec, 5.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13154, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 130, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (122.42, 61.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (161.43, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.360 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13102, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 78, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (86.07, 32.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (160.62, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.171 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13159, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 101, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (98.50, 53.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.79, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.678 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13108, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (186.70, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.60, 71.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.981 CPU sec, 5.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13258, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 92, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (163.36, 120.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 148, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.98, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.241 CPU sec, 5.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13241, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (111.29, 63.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.10, 71.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.495 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13167, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (161.28, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 125.933 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13183, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.67, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.227 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13390, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 59, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (146.60, 160.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.10, 70.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.471 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13272, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 106, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.29, 76.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.87, 70.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.927 CPU sec, 5.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13104, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 105, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (118.14, 65.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.10, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.986 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13203, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 111, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (118.76, 61.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.30, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.173 CPU sec, 4.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13324, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 238, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (179.83, 113.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.29, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.306 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12983, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 133, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (129.62, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (155.67, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.183 CPU sec, 4.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12834, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 85, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (87.17, 49.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (153.67, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.280 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12946, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 89, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (90.20, 39.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.34, 70.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.062 CPU sec, 4.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13007, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (133.27, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (161.20, 72.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.809 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13045, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 87, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (93.44, 49.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.90, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.473 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13025, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 74, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (86.00, 50.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.91, 71.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.163 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13068, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (120.20, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.89, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.113 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13041, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (115.19, 65.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.13, 71.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.005 CPU sec, 6.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13176, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 141, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (149.67, 106.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.36, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.435 CPU sec, 4.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13281, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 92, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (121.75, 79.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.65, 72.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.234 CPU sec, 4.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13204, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 129, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (156.54, 76.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (160.29, 71.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.016 CPU sec, 5.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13184, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 122, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (107.85, 50.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (158.54, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.082 CPU sec, 5.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13327, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 112, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (123.25, 65.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (161.12, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.923 CPU sec, 4.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13323, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 98, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (87.94, 53.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.76, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.903 CPU sec, 4.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13207, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 134, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (128.91, 78.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (155.74, 68.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.701 CPU sec, 4.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13017, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (129.17, 53.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.82, 67.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.044 CPU sec, 4.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12996, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (129.25, 90.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (156.85, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.287 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12800, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 143, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (167.64, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 141, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (153.72, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.152 CPU sec, 5.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12829, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 141, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (135.81, 59.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.63, 68.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.402 CPU sec, 4.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12989, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 172, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (165.30, 83.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.08, 70.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.955 CPU sec, 4.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13082, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 67, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (65.00, 37.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.37, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.046 CPU sec, 4.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13058, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 117, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (131.52, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.06, 70.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.452 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13179, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 138, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (128.38, 72.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.59, 70.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.062 CPU sec, 4.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13200, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (148.40, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.10, 70.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.550 CPU sec, 4.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13125, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 119, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (122.64, 53.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (158.07, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.206 CPU sec, 5.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13151, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.67, 71.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 113.272 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13162, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (111.20, 54.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.34, 69.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.921 CPU sec, 4.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13101, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (133.69, 72.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (157.88, 70.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.510 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13181, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 79, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (79.08, 40.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.29, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.097 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13347, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 81, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (105.80, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.02, 67.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.114 CPU sec, 4.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13233, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 128, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (159.40, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (154.94, 66.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.146 CPU sec, 4.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13141, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 109, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (128.65, 82.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (155.74, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.464 CPU sec, 4.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12976, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 93, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (120.48, 80.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (152.63, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.988 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12925, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 121, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (138.08, 84.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (153.53, 65.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.458 CPU sec, 5.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12926, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 125, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (142.30, 96.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.19, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.427 CPU sec, 5.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13140, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 136, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (131.55, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.65, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.680 CPU sec, 4.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13223, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 86, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (96.06, 48.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.09, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.533 CPU sec, 4.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13056, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 136, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (184.62, 185.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (160.27, 71.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.709 CPU sec, 4.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13078, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.43, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (157.57, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.801 CPU sec, 5.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13277, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.09, 70.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.665 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13195, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.43, 71.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.787 CPU sec, 5.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13106, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 93, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (110.42, 57.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (156.53, 68.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.800 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13333, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 108, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (129.93, 94.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (159.80, 70.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.185 CPU sec, 4.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13066, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 123, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (135.77, 57.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.76, 68.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.302 CPU sec, 4.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13335, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 178, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (187.20, 108.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.47, 70.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.802 CPU sec, 4.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13282, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 237, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (255.64, 172.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.36, 69.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.475 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13308, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 84, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (68.55, 27.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (154.81, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 97.725 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13180, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 135, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (120.06, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (155.31, 68.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.223 CPU sec, 4.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13159, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (118.08, 65.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 141, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (153.60, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.764 CPU sec, 4.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12674, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 141, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (153.07, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.932 CPU sec, 4.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12939, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 194, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (197.45, 125.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (157.11, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.207 CPU sec, 4.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12835, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 82, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (81.50, 42.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.39, 70.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.548 CPU sec, 4.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13192, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.30, 70.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 104.250 CPU sec, 4.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13182, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.04, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 108.718 CPU sec, 4.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13161, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 110, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (120.46, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.41, 70.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.514 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12999, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 84, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (78.25, 42.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.18, 70.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.612 CPU sec, 4.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13025, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (134.29, 81.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.71, 71.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.638 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13044, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (118.08, 56.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.27, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.971 CPU sec, 5.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13219, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 112, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (153.63, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.34, 69.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.843 CPU sec, 4.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13074, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (143.36, 99.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (160.18, 72.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.016 CPU sec, 4.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13071, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (138.16, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (156.76, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.823 CPU sec, 5.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13353, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (147.87, 117.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.45, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.381 CPU sec, 4.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13046, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (104.26, 46.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.07, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.872 CPU sec, 4.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12993, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 132, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (155.91, 95.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (155.48, 66.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.661 CPU sec, 4.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12779, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (109.57, 57.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (154.18, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.712 CPU sec, 3.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12718, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 86, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (102.80, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (153.83, 65.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.698 CPU sec, 4.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13262, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 109, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (97.33, 32.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.04, 68.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.418 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13103, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 139, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (156.30, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.96, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.233 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13075, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 96, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (108.12, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.87, 68.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.549 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13156, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 95, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (117.61, 79.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.60, 68.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.600 CPU sec, 4.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13351, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (133.06, 70.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.93, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.777 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13349, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (162.78, 75.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.94, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.415 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13234, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (155.79, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.40, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.303 CPU sec, 4.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13261, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 117, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (116.40, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.99, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.615 CPU sec, 4.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13154, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (130.92, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (157.38, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.683 CPU sec, 4.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13221, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 68, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (80.40, 45.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.89, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.728 CPU sec, 5.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12912, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (166.80, 94.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (157.87, 68.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.942 CPU sec, 5.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13048, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 132, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (167.82, 152.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.55, 68.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.666 CPU sec, 6.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13254, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 158, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (160.09, 82.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.28, 69.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.549 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13153, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 85, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (126.06, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.42, 69.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.976 CPU sec, 5.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13126, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 113, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (117.57, 57.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.54, 71.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.874 CPU sec, 5.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13240, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 108, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (110.65, 53.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.58, 70.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.557 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13433, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 178, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (191.54, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (154.99, 67.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.308 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13157, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 230, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (214.86, 149.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.21, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.125 CPU sec, 4.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13240, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 152, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (169.91, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.83, 68.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.281 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13205, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (133.93, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (155.85, 67.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.085 CPU sec, 4.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13330, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (156.93, 68.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.894 CPU sec, 5.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13188, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 107, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (137.85, 78.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.30, 71.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.661 CPU sec, 5.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13192, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 137, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (151.50, 86.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.59, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.191 CPU sec, 6.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13115, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 132, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (137.00, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (158.67, 70.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.835 CPU sec, 4.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13184, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 92, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (124.80, 83.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.40, 68.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.753 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13121, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 118, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (135.86, 103.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.61, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.584 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13042, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 79, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (83.36, 39.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.00, 68.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.293 CPU sec, 4.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13093, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 143, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (146.92, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.23, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.005 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12987, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (126.73, 62.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.54, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.828 CPU sec, 4.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13134, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 134, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (113.57, 60.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.95, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.059 CPU sec, 4.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13292, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 118, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (109.00, 42.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (161.29, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.228 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13455, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 129, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (133.60, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.78, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.936 CPU sec, 4.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13131, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.08, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.820 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13219, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 81, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (114.87, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (155.20, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.734 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13212, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 149, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (154.67, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.38, 66.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.914 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13235, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 116, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (138.38, 85.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (154.73, 66.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.365 CPU sec, 5.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13185, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (129.47, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (160.00, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.975 CPU sec, 4.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13254, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 149, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (134.08, 76.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (158.99, 71.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.955 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13169, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 100, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (130.00, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (157.50, 70.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.651 CPU sec, 4.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13255, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (122.95, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.02, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.981 CPU sec, 5.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13232, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 95, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (106.17, 61.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.79, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.821 CPU sec, 4.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12992, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.75, 69.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.737 CPU sec, 6.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12997, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 110, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (122.14, 61.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.80, 70.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.697 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13123, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 68, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (79.00, 51.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.56, 71.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.981 CPU sec, 6.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13047, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 92, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 192) [M::mem_pestat] mean and std.dev: (93.56, 36.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.46, 70.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.782 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13206, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.89, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.312 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13095, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 185, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (161.80, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.39, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.364 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13126, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (126.46, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.58, 67.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.333 CPU sec, 5.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13189, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 82, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (87.60, 39.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.30, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.439 CPU sec, 6.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13174, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 121, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (125.33, 45.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.08, 66.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.429 CPU sec, 5.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13058, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 126, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (136.81, 75.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.13, 67.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.080 CPU sec, 5.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13330, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 96, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (107.75, 52.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.49, 71.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.242 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13144, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 103, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (99.75, 47.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (158.24, 69.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.781 CPU sec, 5.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13049, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 123, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (128.62, 65.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.07, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.721 CPU sec, 6.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13065, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 169, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (143.00, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (157.30, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.800 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13228, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (147.93, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.20, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.605 CPU sec, 5.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13095, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 131, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (133.92, 103.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (154.78, 66.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.578 CPU sec, 5.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12942, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (128.07, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (155.89, 67.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.828 CPU sec, 5.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13086, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (139.35, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.24, 68.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.531 CPU sec, 4.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13107, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 78, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (71.15, 26.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 142, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (153.89, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.084 CPU sec, 5.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13331, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 129, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (125.50, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.93, 69.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.380 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13298, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 94, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 161) [M::mem_pestat] mean and std.dev: (86.43, 21.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.46, 69.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.629 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13308, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 91, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (134.36, 109.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.96, 67.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.787 CPU sec, 4.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13289, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 76, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 130) [M::mem_pestat] mean and std.dev: (61.89, 18.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.29, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.490 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13026, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.04, 67.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.843 CPU sec, 6.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13177, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (107.08, 55.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (156.14, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.064 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13195, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (125.20, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (157.08, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.867 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13355, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 95, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (144.00, 118.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.28, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.630 CPU sec, 4.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13199, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 111, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (116.22, 54.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.57, 69.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.524 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13248, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (106.92, 66.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.48, 69.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.704 CPU sec, 4.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13205, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 142, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (173.90, 89.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (156.43, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.725 CPU sec, 5.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13240, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 83, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (106.00, 60.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (157.76, 70.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.778 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13098, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 91, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (125.00, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.80, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.186 CPU sec, 6.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13145, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 84, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (123.92, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.75, 69.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.858 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13192, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 98, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (101.90, 58.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (154.47, 66.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.593 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13079, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 86, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (126.77, 83.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.21, 68.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.062 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13052, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 137, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (128.79, 50.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.70, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.988 CPU sec, 5.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13325, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 118, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (126.12, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.87, 69.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.965 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13254, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 105, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (130.76, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (156.17, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.130 CPU sec, 6.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13207, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 100, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (80.76, 41.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (157.67, 70.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.950 CPU sec, 6.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13196, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (153.71, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.716 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13084, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.46, 67.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.748 CPU sec, 4.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13473, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 100, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (117.00, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (157.61, 70.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.971 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13257, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 87, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (82.55, 50.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (156.97, 68.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.023 CPU sec, 4.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13276, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (125.38, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (156.86, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.671 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13215, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 78, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (96.12, 58.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.80, 69.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.791 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13192, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (157.36, 68.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.071 CPU sec, 4.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12988, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (155.83, 68.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.116 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13009, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 123, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (101.27, 34.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.03, 67.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.381 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13184, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 103, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (89.25, 25.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.77, 68.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.285 CPU sec, 5.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13089, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 85, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (157.82, 120.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.50, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.856 CPU sec, 4.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13091, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 90, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (95.07, 46.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.42, 68.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.614 CPU sec, 6.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13101, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.66, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.554 CPU sec, 6.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13278, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 154, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (173.83, 100.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.79, 68.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.450 CPU sec, 5.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13097, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 117, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (127.00, 61.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.46, 67.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.931 CPU sec, 5.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13117, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (100.64, 49.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (154.99, 66.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.630 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13087, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 98, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (96.92, 43.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (155.59, 66.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.769 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12897, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (118.64, 41.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.28, 66.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.550 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13192, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 137, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (143.65, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (158.77, 71.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.518 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13239, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (129.07, 76.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.06, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.664 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13122, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 81, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (111.58, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.01, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.195 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13252, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 134, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (134.91, 95.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (158.90, 70.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.271 CPU sec, 5.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13224, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 93, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (147.23, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (158.78, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.411 CPU sec, 4.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13161, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 107, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (111.56, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.71, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.742 CPU sec, 6.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13092, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (102.91, 57.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.31, 69.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.893 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13273, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.69, 72.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.312 CPU sec, 5.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13421, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 110, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (129.46, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.62, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.445 CPU sec, 6.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13038, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 91, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (94.00, 59.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.13, 70.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.587 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13268, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 84, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (91.60, 31.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.69, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.513 CPU sec, 5.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13133, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 93, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (119.25, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.90, 69.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.921 CPU sec, 5.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13157, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 95, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (128.57, 98.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.19, 67.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.219 CPU sec, 5.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12992, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 92, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (86.11, 42.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (153.86, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.193 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12794, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 93, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (105.33, 70.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (157.12, 69.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.560 CPU sec, 6.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12883, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 63, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (118.73, 92.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.34, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.083 CPU sec, 5.994 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13192, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 139, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (131.55, 70.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.17, 70.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.605 CPU sec, 5.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13031, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 114, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (135.71, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.48, 70.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.761 CPU sec, 5.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13247, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 179, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (164.40, 115.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (155.98, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.350 CPU sec, 5.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13133, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 110, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (124.88, 61.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.30, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.431 CPU sec, 5.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13241, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 111, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (111.08, 36.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.42, 69.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.676 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13073, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.32, 70.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.373 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13221, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (108.09, 66.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.12, 70.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.057 CPU sec, 5.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13140, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 130, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (169.00, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.22, 70.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.448 CPU sec, 5.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13106, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 142, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (160.29, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (158.73, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.392 CPU sec, 5.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13171, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (129.56, 74.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.04, 69.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.525 CPU sec, 5.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13256, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 147, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (157.17, 86.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (158.38, 71.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.044 CPU sec, 6.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13206, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.78, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.015 CPU sec, 5.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12917, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 110, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (108.65, 62.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (155.61, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.428 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12963, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 94, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (114.67, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (155.55, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.852 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12668, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (28, 57, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (53.60, 42.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (154.67, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.660 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13090, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 103, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (133.27, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (157.72, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.601 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13069, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 103, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (102.69, 46.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.08, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.504 CPU sec, 6.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13175, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (134.62, 105.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.74, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.189 CPU sec, 6.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13367, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 157, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (144.33, 91.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.02, 69.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.881 CPU sec, 5.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13190, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 142, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.75, 55.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.46, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.165 CPU sec, 6.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13144, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.73, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.009 CPU sec, 4.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13203, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (143.42, 113.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (157.19, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.215 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13187, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 141, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (123.61, 68.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (160.70, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.881 CPU sec, 4.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13171, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (132.12, 105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.26, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.141 CPU sec, 5.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13216, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 113, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (136.42, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.45, 71.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.418 CPU sec, 5.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13146, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.80, 69.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.890 CPU sec, 4.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13115, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (106.13, 64.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (154.52, 66.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.115 CPU sec, 4.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12964, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 151, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (149.92, 89.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (153.94, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.405 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12995, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 94, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (88.90, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (153.44, 64.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.758 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12808, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 117, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (110.11, 48.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 141, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (153.48, 65.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.887 CPU sec, 6.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12862, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 98, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (107.00, 67.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 141, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (154.50, 66.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.916 CPU sec, 5.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13103, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 118, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (138.71, 72.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (156.34, 68.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.398 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13182, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 150, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (154.80, 80.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.87, 69.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.031 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13190, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (135.56, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.77, 68.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.089 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13219, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.36, 68.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.740 CPU sec, 5.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13215, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 97, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (142.00, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (155.21, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.538 CPU sec, 4.785 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13162, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (138.33, 50.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (158.84, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.252 CPU sec, 5.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13181, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 80, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 146) [M::mem_pestat] mean and std.dev: (78.10, 25.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.74, 70.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.994 CPU sec, 5.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13068, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.30, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.272 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12932, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 75, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (62.00, 23.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.36, 70.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.873 CPU sec, 6.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13334, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (133.40, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.89, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.310 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13145, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 139, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (137.41, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (155.88, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.641 CPU sec, 5.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13141, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 125, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (144.31, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.51, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.642 CPU sec, 4.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13117, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 85, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (82.38, 29.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 141, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (153.71, 65.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.419 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12885, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (89.67, 29.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (154.87, 65.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.144 CPU sec, 5.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12687, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 141, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (152.33, 63.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.107 CPU sec, 6.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13155, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 126, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (127.81, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.41, 70.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.469 CPU sec, 6.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13245, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 89, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (104.05, 54.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (156.95, 67.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.843 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13075, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (103.79, 40.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (156.96, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.290 CPU sec, 5.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13260, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 88, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (88.10, 43.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (157.17, 68.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.850 CPU sec, 5.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13036, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (88.33, 41.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (155.02, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.142 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13229, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 109, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (112.00, 60.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.75, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.923 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13156, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 121, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (93.38, 29.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (158.79, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.871 CPU sec, 5.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13222, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (107.55, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.10, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.725 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13157, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.91, 71.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.664 CPU sec, 5.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12958, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 158, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (138.22, 81.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.08, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.419 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13127, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 165, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (154.45, 59.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.26, 70.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.338 CPU sec, 5.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13277, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 100, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (110.45, 71.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (156.47, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.703 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13254, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 92, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (113.14, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (155.45, 66.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.898 CPU sec, 4.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13103, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 101, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (95.14, 51.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (154.98, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.179 CPU sec, 5.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13029, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 99, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (102.07, 50.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (153.86, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.374 CPU sec, 5.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13020, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 75, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (103.15, 92.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (156.17, 68.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.989 CPU sec, 5.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13189, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 216, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (197.00, 121.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.33, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.614 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13257, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 104, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (113.70, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.48, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.641 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13322, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 80, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (89.44, 53.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (159.27, 70.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.352 CPU sec, 5.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13340, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 117, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (113.44, 40.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.19, 70.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.499 CPU sec, 5.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13079, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (159.25, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.784 CPU sec, 5.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13197, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (134.14, 62.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (158.12, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.638 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13309, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.61, 69.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.579 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13100, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 92, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (113.00, 63.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.04, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.496 CPU sec, 4.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13046, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 271) [M::mem_pestat] mean and std.dev: (133.47, 37.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (160.30, 72.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.251 CPU sec, 6.337 real sec [M::process] read 13074 sequences (1974174 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13110, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 95, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (111.38, 49.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (157.92, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.455 CPU sec, 5.177 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 2642, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.98, 68.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 13074 reads in 31.481 CPU sec, 2.502 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21154\tSM:UG10S2P088\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21154_HLKNYBGXX_UG10S2P088_R1.fastq.gz 5347_7332_21154_HLKNYBGXX_UG10S2P088_R2.fastq.gz [main] Real time: 1974.202 sec; CPU: 47829.317 sec Finished alignement of UG10S2P088 at Wed Apr 3 03:00:26 2019 Sorting BAM file started at Wed Apr 3 03:00:26 2019 samtools sort -@ 30 -m 4G UG10S2P088_unsorted.bam -o UG10S2P088.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 03:00:26 2019 rm UG10S2P088_unsorted.bam rm 5347_7332_21154_HLKNYBGXX_UG10S2P088_R1.fastq.gz rm 5347_7332_21154_HLKNYBGXX_UG10S2P088_R2.fastq.gz samtools index UG10S2P088.bam Finished BAM file generation of the sample - UG10S2P088 Processing fastq files for the sample - UG10S2P089 Reading 5347_7332_21155_HLKNYBGXX_UG10S2P089_R1.fastq.gz 5347_7332_21155_HLKNYBGXX_UG10S2P089_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21155\tSM:UG10S2P089\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21155_HLKNYBGXX_UG10S2P089_R1.fastq.gz 5347_7332_21155_HLKNYBGXX_UG10S2P089_R2.fastq.gz | samtools view -Sb1 - > UG10S2P089_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12434, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 169, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (217.80, 156.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 177, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (220.21, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.238 CPU sec, 5.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12537, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 162, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (207.38, 171.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (220.43, 128.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.401 CPU sec, 4.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12586, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 161, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (155.59, 103.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 181, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (226.17, 131.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.817 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12705, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 168, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (152.77, 131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (219.88, 128.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.397 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12609, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 106, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (127.79, 86.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (217.56, 125.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.784 CPU sec, 6.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12367, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 143, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (170.50, 114.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (215.22, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.101 CPU sec, 5.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12459, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 121, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (180.67, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (214.00, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.732 CPU sec, 5.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12342, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 105, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (109.58, 55.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (217.23, 125.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.438 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12523, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 180, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (165.20, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (224.50, 131.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.421 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12674, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 125, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (136.39, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 177, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (220.61, 128.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.415 CPU sec, 5.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12655, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 144, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (162.17, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 177, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (220.09, 127.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.280 CPU sec, 5.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12573, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (151.63, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 175, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (217.76, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.032 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12445, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 123, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (171.28, 132.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (211.06, 118.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.354 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12460, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (143.54, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (219.25, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.576 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12400, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 150, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (244.55, 228.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 176, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (219.25, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.541 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12485, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 172, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (166.80, 89.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 176, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (221.11, 128.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.456 CPU sec, 5.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12588, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 178, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (220.02, 126.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.762 CPU sec, 6.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12489, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 147, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 296) [M::mem_pestat] mean and std.dev: (137.00, 36.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (217.80, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.971 CPU sec, 4.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12439, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (228.06, 112.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (211.27, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.523 CPU sec, 4.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12396, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 93, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (157.00, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (208.75, 117.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.299 CPU sec, 4.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12499, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 122, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (152.94, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (214.25, 121.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.896 CPU sec, 6.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12608, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 175, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (217.55, 125.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.730 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12482, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 145, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (127.00, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 177, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (220.78, 126.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.604 CPU sec, 5.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12560, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 218, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (217.50, 141.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (219.88, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.517 CPU sec, 4.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12422, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 120, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (133.92, 89.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (213.01, 120.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.057 CPU sec, 5.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12376, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (180.81, 93.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 172, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (212.60, 120.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.858 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12276, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 138, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (169.75, 88.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (210.44, 116.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.723 CPU sec, 5.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12345, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 263, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (212.50, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (212.23, 119.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.503 CPU sec, 6.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12598, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (114.08, 55.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (215.32, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.544 CPU sec, 4.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12528, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 111, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (115.64, 47.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (219.57, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.437 CPU sec, 5.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12517, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (147.00, 85.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 175, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (217.27, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.186 CPU sec, 5.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12471, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 186, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (162.50, 61.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.70, 119.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.315 CPU sec, 5.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12409, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (198.62, 102.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (212.19, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.610 CPU sec, 5.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12447, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 157, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (165.47, 103.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 169, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (208.13, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.763 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12384, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (184.54, 93.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (215.74, 123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.779 CPU sec, 5.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12594, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 140, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.36, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (216.93, 123.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.799 CPU sec, 5.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12515, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 90, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (89.91, 42.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 171, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (211.41, 120.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.897 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12516, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 113, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (191.07, 142.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (212.55, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.282 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12449, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (210.13, 117.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.830 CPU sec, 4.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12423, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 183, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (201.53, 122.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (207.95, 114.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.546 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12531, 6, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 179, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (220.81, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.899 CPU sec, 4.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12733, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 159, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (167.82, 94.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 177, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (222.31, 128.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.275 CPU sec, 5.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12628, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 210, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (215.36, 93.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (219.30, 125.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.708 CPU sec, 4.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12696, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 106, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (138.00, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (220.84, 127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.658 CPU sec, 4.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12421, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 198, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (214.32, 154.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (219.34, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.190 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12339, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 109, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (131.00, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (212.06, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.088 CPU sec, 5.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12138, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 144, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (190.21, 115.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (209.06, 117.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.607 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12546, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 178, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (223.50, 129.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.702 CPU sec, 5.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12656, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 181, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (227.84, 132.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.424 CPU sec, 5.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12710, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 82, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (134.20, 89.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 177, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (221.01, 127.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.897 CPU sec, 6.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12651, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 172, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (222.62, 155.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (220.71, 127.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.463 CPU sec, 4.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12562, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 140, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (160.95, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (215.05, 122.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.447 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12436, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 142, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (130.33, 67.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (203.76, 113.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.114 CPU sec, 5.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12142, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 134, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (145.87, 94.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (216.75, 123.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.705 CPU sec, 4.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12709, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 177, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (168.14, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (220.99, 127.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.553 CPU sec, 4.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12672, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 181, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (224.20, 129.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.993 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12610, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (136.76, 87.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 177, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (223.36, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.313 CPU sec, 5.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12475, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 87, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (151.36, 137.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 176, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (221.71, 129.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.968 CPU sec, 5.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12487, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (208.80, 115.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.523 CPU sec, 4.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11918, 9, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 147, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (138.56, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (206.34, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.032 CPU sec, 5.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12266, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 97, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (119.60, 84.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (218.42, 125.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.813 CPU sec, 6.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12658, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 189, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (169.39, 118.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (217.72, 124.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.454 CPU sec, 5.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12488, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 223, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (190.17, 95.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (218.86, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.167 CPU sec, 5.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12595, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 91, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (169.40, 143.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (217.81, 125.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.055 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12323, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 80, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (76.67, 22.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (212.40, 119.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.690 CPU sec, 5.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12241, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 54, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (108.46, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (206.84, 115.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.511 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12081, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (120.74, 62.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (202.43, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.179 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12554, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (127.14, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 180, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (224.17, 130.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.792 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12572, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (203.92, 118.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 176, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (220.49, 126.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.906 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12632, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 157, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (205.36, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (219.97, 127.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.060 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12603, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 105, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (137.36, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (216.70, 124.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.645 CPU sec, 4.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12464, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (186.00, 105.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (210.97, 118.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.226 CPU sec, 5.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12337, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (202.11, 110.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.606 CPU sec, 5.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12075, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 185, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (133.70, 113.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (212.12, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.911 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12472, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (171.69, 78.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 177, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (222.17, 127.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.317 CPU sec, 5.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12637, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 172, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (164.33, 68.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 177, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (219.73, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.018 CPU sec, 5.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12499, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (220.89, 127.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.690 CPU sec, 4.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12445, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 144, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (135.23, 63.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (218.18, 124.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.313 CPU sec, 5.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12361, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 89, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (97.06, 52.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (207.31, 114.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.357 CPU sec, 4.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11772, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 111, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (114.23, 36.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (199.26, 106.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.441 CPU sec, 5.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12494, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 91, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (157.91, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (209.81, 118.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.797 CPU sec, 5.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12424, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 118, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (121.17, 65.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (207.87, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.589 CPU sec, 5.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12637, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 153, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (197.20, 117.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 177, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (220.82, 127.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.850 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12656, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 131, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (158.40, 84.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (216.56, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.149 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12410, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 138, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (155.30, 101.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (214.92, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.303 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12366, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (220.21, 127.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.958 CPU sec, 4.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12447, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 124, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (151.86, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (216.06, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.297 CPU sec, 4.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12414, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (157.40, 89.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (211.75, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.426 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12610, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 71, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (79.25, 43.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (219.50, 126.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.289 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12605, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (161.63, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (216.47, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.467 CPU sec, 5.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12295, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (216.44, 125.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.378 CPU sec, 4.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12350, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 146, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (153.10, 104.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (215.28, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.004 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12463, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (179.53, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (216.83, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.748 CPU sec, 6.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12536, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 111, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (149.64, 99.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (209.12, 116.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.876 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12433, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 142, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (134.70, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (205.87, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.803 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12664, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 81, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (127.25, 84.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (220.64, 128.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.315 CPU sec, 4.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12628, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 179, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (168.44, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (216.62, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.390 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12335, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 191, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (175.09, 108.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (216.63, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.445 CPU sec, 6.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12552, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (219.99, 126.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.997 CPU sec, 6.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12649, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (140.85, 81.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (213.82, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.949 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12432, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (175.00, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (207.98, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.706 CPU sec, 5.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12615, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 127, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (127.67, 57.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (213.41, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.276 CPU sec, 4.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12727, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (148.56, 52.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (216.50, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.007 CPU sec, 5.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12483, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (177.35, 83.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (209.93, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.160 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12513, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 146, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (134.50, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (208.51, 114.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.762 CPU sec, 5.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12502, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (127.50, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (214.78, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.170 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12615, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 316, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (247.70, 166.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (212.99, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.053 CPU sec, 5.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12318, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 152, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (170.86, 128.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (209.13, 116.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.618 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12472, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (217.18, 124.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.987 CPU sec, 6.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12417, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 234, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (203.58, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 174, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (215.24, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.232 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12460, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 90, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (117.00, 74.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (207.99, 115.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.029 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12387, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 220, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (227.09, 112.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (213.20, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.941 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12409, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 202, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (167.87, 77.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 171, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (210.83, 118.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.276 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12519, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 141, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (125.00, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (203.65, 110.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.383 CPU sec, 5.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12364, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 72, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (119.87, 90.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (212.14, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.266 CPU sec, 5.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12597, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 80, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (126.50, 128.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (212.73, 119.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.363 CPU sec, 5.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12335, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 129, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (118.31, 40.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (209.57, 118.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.448 CPU sec, 5.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12327, 1, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (211.05, 118.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.499 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12418, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 107, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (130.79, 89.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (214.53, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.652 CPU sec, 5.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12263, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (206.44, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.498 CPU sec, 5.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12124, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 168, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (171.36, 93.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (203.73, 111.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.321 CPU sec, 5.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12552, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (91.50, 41.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (219.15, 126.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.500 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12627, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (176.16, 141.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (214.59, 122.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.969 CPU sec, 6.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12476, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 182, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (161.89, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (217.39, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.294 CPU sec, 5.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12507, 3, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 178, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (221.07, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.437 CPU sec, 6.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12476, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (189.00, 112.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (212.48, 119.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.995 CPU sec, 4.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11915, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 150, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (183.56, 118.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (203.38, 111.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.669 CPU sec, 5.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12460, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 210, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (209.64, 108.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (218.45, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.783 CPU sec, 5.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12479, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 121, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (135.18, 75.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 176, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (219.91, 127.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.892 CPU sec, 5.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12368, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 120, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (105.23, 44.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (218.02, 125.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.452 CPU sec, 5.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12724, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (112.47, 47.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 178, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (223.04, 129.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.745 CPU sec, 5.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12399, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 140, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (122.93, 48.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (218.03, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.426 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12341, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (210.18, 115.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.270 CPU sec, 5.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12080, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 179, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (167.75, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (208.86, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.436 CPU sec, 7.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12498, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 111, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (107.93, 48.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (219.12, 127.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.037 CPU sec, 4.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12627, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 128, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (145.42, 100.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (213.71, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.966 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12704, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 111, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (118.92, 57.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 174, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (216.47, 123.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.411 CPU sec, 6.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12538, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 155, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (163.56, 118.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (220.19, 127.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.534 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12389, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 170, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (193.19, 105.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (205.19, 111.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.781 CPU sec, 5.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11857, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 102, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (143.52, 122.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (196.00, 102.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.746 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12565, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 136, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (144.30, 77.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (213.55, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.560 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12470, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 191, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (150.75, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 172, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (212.57, 119.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.644 CPU sec, 5.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12445, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (157.71, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (212.73, 120.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.718 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12542, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 101, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (107.57, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (216.59, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.782 CPU sec, 5.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12443, 11, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 72, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (76.18, 35.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (213.58, 120.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (342, 3805, 4434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12618) [M::mem_pestat] mean and std.dev: (2925.36, 2727.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16710) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 130.810 CPU sec, 5.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12097, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 111, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (103.77, 37.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (199.52, 108.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.258 CPU sec, 4.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12237, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 141, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (176.42, 143.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (201.85, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.326 CPU sec, 6.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12662, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 230, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (210.70, 70.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (215.33, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.604 CPU sec, 5.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12388, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 143, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (149.62, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (211.71, 118.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.206 CPU sec, 4.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12562, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (142.10, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 175, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (215.63, 121.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.567 CPU sec, 6.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12545, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 106, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (128.93, 68.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 176, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (217.62, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.723 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12482, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 146, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (191.70, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (203.94, 112.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.084 CPU sec, 5.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11999, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 143, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (204.21, 164.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (200.01, 107.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.850 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12698, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 165, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (143.60, 67.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (214.18, 121.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.915 CPU sec, 5.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12587, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (141.78, 99.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (216.16, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.793 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12547, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 172, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (236.13, 213.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 176, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (217.55, 124.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.193 CPU sec, 5.389 real sec [M::process] read 21764 sequences (3286364 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12547, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 127, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (137.33, 80.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 177, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (217.79, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.025 CPU sec, 4.750 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 4136, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 174, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (216.64, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 21764 reads in 42.166 CPU sec, 1.893 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21155\tSM:UG10S2P089\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21155_HLKNYBGXX_UG10S2P089_R1.fastq.gz 5347_7332_21155_HLKNYBGXX_UG10S2P089_R2.fastq.gz [main] Real time: 851.782 sec; CPU: 21279.947 sec Finished alignement of UG10S2P089 at Wed Apr 3 03:35:19 2019 Sorting BAM file started at Wed Apr 3 03:35:19 2019 samtools sort -@ 30 -m 4G UG10S2P089_unsorted.bam -o UG10S2P089.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 03:35:19 2019 rm UG10S2P089_unsorted.bam rm 5347_7332_21155_HLKNYBGXX_UG10S2P089_R1.fastq.gz rm 5347_7332_21155_HLKNYBGXX_UG10S2P089_R2.fastq.gz samtools index UG10S2P089.bam Finished BAM file generation of the sample - UG10S2P089 Processing fastq files for the sample - UG10S2P090 Reading 5347_7332_21156_HLKNYBGXX_UG10S2P090_R1.fastq.gz 5347_7332_21156_HLKNYBGXX_UG10S2P090_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21156\tSM:UG10S2P090\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21156_HLKNYBGXX_UG10S2P090_R1.fastq.gz 5347_7332_21156_HLKNYBGXX_UG10S2P090_R2.fastq.gz | samtools view -Sb1 - > UG10S2P090_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13046, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (130.81, 49.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.334 CPU sec, 5.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13105, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (134.05, 53.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.831 CPU sec, 5.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12912, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.53, 52.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.023 CPU sec, 6.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13088, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 103, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (217.70, 272.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.94, 52.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.269 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12970, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (118.36, 68.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.79, 52.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.585 CPU sec, 6.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12940, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (131.64, 51.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.218 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13116, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.05, 51.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.124 CPU sec, 5.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12958, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.36, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.483 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13173, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (131.24, 50.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.903 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12890, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.87, 50.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.304 CPU sec, 7.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12928, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 190, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (199.83, 125.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (134.05, 54.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.690 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13086, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (99.25, 32.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.85, 53.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.995 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13099, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.27, 52.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.905 CPU sec, 5.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13094, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 86, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (111.88, 58.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (133.00, 52.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.602 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12988, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 239, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (207.80, 137.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (131.96, 51.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.110 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13139, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 129, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (135.67, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.80, 53.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.687 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13043, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 141, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (144.31, 76.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.51, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.527 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13139, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 118, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (143.09, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.66, 49.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.011 CPU sec, 6.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13169, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 133, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (160.71, 100.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.41, 50.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.826 CPU sec, 5.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13047, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.28, 50.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.635 CPU sec, 5.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12954, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 152, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (159.18, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.85, 51.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.251 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13066, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (131.95, 51.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.743 CPU sec, 5.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12918, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.01, 52.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.014 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13007, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (110.64, 58.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.76, 50.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.871 CPU sec, 5.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12950, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (132.61, 52.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.663 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13105, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.78, 50.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.409 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13246, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 132, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (123.64, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.69, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.213 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13018, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (131.48, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.734 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12981, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (131.27, 51.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.185 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12976, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.67, 50.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.250 CPU sec, 5.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13064, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 121, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (143.40, 108.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (132.80, 53.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.936 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12985, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.68, 52.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.910 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13121, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (132.18, 51.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.655 CPU sec, 5.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12999, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (130.20, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.92, 52.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.411 CPU sec, 7.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12892, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (130.84, 50.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.830 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12887, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 74, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (93.89, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.40, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.129 CPU sec, 5.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13058, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.13, 51.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.181 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13145, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 130, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (172.27, 116.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.50, 50.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.882 CPU sec, 5.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12919, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 74, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (61.92, 23.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.97, 50.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.035 CPU sec, 5.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12808, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.33, 49.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.198 CPU sec, 5.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12912, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (133.07, 52.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.237 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12950, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.50, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.658 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13034, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.89, 51.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.251 CPU sec, 6.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12929, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 81, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (107.27, 80.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.26, 50.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.719 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13127, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 100, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (103.00, 63.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.46, 52.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.946 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13027, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 125, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (183.60, 132.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.99, 50.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.167 CPU sec, 6.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13221, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.82, 50.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.378 CPU sec, 5.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13237, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (129.97, 49.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.846 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13141, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.82, 50.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.530 CPU sec, 5.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12905, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (97.79, 51.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (130.24, 50.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.288 CPU sec, 5.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12802, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 78, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (111.42, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.75, 51.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.893 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13091, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.33, 50.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.545 CPU sec, 5.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12912, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (204.50, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.34, 51.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.583 CPU sec, 6.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12807, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (129.85, 49.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.550 CPU sec, 7.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12918, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 74, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (140.73, 103.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (131.30, 51.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.242 CPU sec, 6.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13074, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 88, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (116.50, 66.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.26, 49.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.497 CPU sec, 7.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13024, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 127, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (155.42, 116.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.12, 48.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.411 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13015, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.66, 49.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.437 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12922, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (134.54, 53.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.518 CPU sec, 5.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12965, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.65, 52.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.784 CPU sec, 5.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12843, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 85, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (135.50, 132.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 127, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (133.91, 54.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.049 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13035, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.42, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.791 CPU sec, 5.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13052, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.31, 52.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.902 CPU sec, 6.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12834, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 117, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (141.56, 75.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (133.66, 53.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.108 CPU sec, 5.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13085, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 146, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (139.58, 71.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.21, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.684 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13099, 3, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.02, 52.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.319 CPU sec, 5.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12917, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (131.26, 50.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.097 CPU sec, 5.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12685, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 82, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (82.30, 28.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (129.74, 49.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.928 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13113, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 90, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (108.00, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (133.28, 53.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.273 CPU sec, 5.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13056, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.32, 52.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.305 CPU sec, 5.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13132, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 110, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (146.00, 101.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (133.17, 53.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.530 CPU sec, 6.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12812, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.59, 52.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.935 CPU sec, 5.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13151, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 139, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (179.47, 147.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.48, 53.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.470 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13103, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 78, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (79.38, 46.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (133.03, 53.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.983 CPU sec, 6.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13008, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 157, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (145.79, 110.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.88, 51.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.902 CPU sec, 6.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13070, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 174, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (146.92, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.74, 50.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.541 CPU sec, 4.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12692, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.56, 49.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.177 CPU sec, 5.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12987, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 197, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (148.33, 58.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.06, 52.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.285 CPU sec, 5.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12939, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 174, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (195.60, 137.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (134.09, 54.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.591 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12997, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 86, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (107.69, 66.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (132.85, 52.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.597 CPU sec, 5.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12948, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (133.32, 53.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.846 CPU sec, 5.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13150, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (131.80, 51.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.148 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12795, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (131.90, 51.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.981 CPU sec, 6.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12985, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.91, 50.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.496 CPU sec, 5.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13183, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 172, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (181.00, 117.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.86, 50.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.511 CPU sec, 6.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13024, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (130.74, 50.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.001 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12870, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 175, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (198.60, 113.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.16, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.483 CPU sec, 5.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12990, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (133.65, 53.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.550 CPU sec, 5.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12989, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 88, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (140.10, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.58, 50.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.832 CPU sec, 6.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13072, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 174, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (190.90, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.86, 52.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.342 CPU sec, 6.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12964, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.56, 51.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.058 CPU sec, 6.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 12881, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.91, 51.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.934 CPU sec, 5.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12950, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 134, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (172.15, 109.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.57, 51.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.697 CPU sec, 5.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13104, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (130.18, 49.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.867 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13047, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 85, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (68.89, 30.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.34, 49.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.313 CPU sec, 5.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13059, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (130.21, 50.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.701 CPU sec, 5.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13006, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.82, 50.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.595 CPU sec, 5.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12940, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.19, 52.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.898 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12812, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 172, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (178.50, 138.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (131.89, 51.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.834 CPU sec, 5.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12943, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (112.08, 69.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.36, 51.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.658 CPU sec, 6.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12910, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 158, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (179.00, 104.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.28, 52.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.589 CPU sec, 5.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 13054, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.45, 52.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.641 CPU sec, 6.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13104, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.83, 51.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.182 CPU sec, 5.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12995, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (130.40, 49.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.645 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13001, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (130.25, 49.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 125.777 CPU sec, 4.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12954, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 62, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (80.10, 43.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (129.90, 49.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.842 CPU sec, 4.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13056, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.13, 51.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.233 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12935, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.08, 52.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.056 CPU sec, 5.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12807, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (85.75, 24.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.63, 52.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.156 CPU sec, 6.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12744, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 98, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (78.25, 45.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.02, 51.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.293 CPU sec, 6.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12793, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.75, 52.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.222 CPU sec, 7.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12854, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 93, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (101.62, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.41, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.905 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12953, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 156, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (166.85, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.42, 50.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.622 CPU sec, 6.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13136, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (130.88, 51.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.953 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13100, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.74, 49.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.776 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13065, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 84, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (82.56, 26.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (130.63, 51.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.085 CPU sec, 5.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13130, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.78, 50.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.958 CPU sec, 4.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13038, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.26, 50.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.475 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12940, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 79, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (102.42, 72.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (132.54, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.145 CPU sec, 6.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12946, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (67.33, 46.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (133.96, 54.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.765 CPU sec, 5.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13183, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 121, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (108.00, 46.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (133.06, 53.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.555 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 13128, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (131.12, 49.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.667 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13025, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 166, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (151.80, 112.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.10, 50.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.721 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12947, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (131.81, 50.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.228 CPU sec, 6.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13077, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (131.91, 52.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.548 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13158, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (133.16, 52.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.093 CPU sec, 4.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13113, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 68, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (90.82, 53.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (131.17, 50.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.477 CPU sec, 5.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13223, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.19, 50.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.929 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13181, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.05, 52.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.377 CPU sec, 5.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12875, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (116.10, 52.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.93, 52.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.485 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13119, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 121, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (154.17, 122.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.43, 52.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.739 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12842, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.95, 52.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.915 CPU sec, 5.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13087, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 122, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (150.27, 101.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.27, 51.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.203 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12859, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 69, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (95.69, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.23, 51.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.575 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13328, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 79, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (80.92, 54.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (130.76, 50.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.357 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13182, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (131.03, 50.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.022 CPU sec, 4.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13004, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 120, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (137.25, 85.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.76, 51.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.856 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13091, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 87, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 167) [M::mem_pestat] mean and std.dev: (70.38, 27.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.85, 53.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.928 CPU sec, 5.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13182, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 76, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (87.60, 59.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.74, 53.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.694 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13128, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.13, 51.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.455 CPU sec, 5.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13039, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 102, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (113.36, 53.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (131.59, 50.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.376 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13017, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (131.99, 51.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.685 CPU sec, 5.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13035, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 93, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (95.93, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.64, 51.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.865 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12939, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 150, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (157.09, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.41, 51.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.488 CPU sec, 6.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13254, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (84.89, 38.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.79, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.003 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12949, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 162, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (164.45, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (131.07, 50.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.400 CPU sec, 5.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12837, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.76, 52.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.694 CPU sec, 6.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12985, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 93, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (80.44, 40.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.47, 50.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.542 CPU sec, 6.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12843, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 91, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (92.90, 40.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (131.32, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.972 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12871, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 131, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (112.42, 33.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (130.56, 50.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.673 CPU sec, 6.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12977, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (121.44, 67.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (129.75, 49.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.064 CPU sec, 5.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13037, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.20, 51.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.366 CPU sec, 5.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13109, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 90, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (93.70, 54.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.70, 51.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.355 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13229, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 150, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (175.50, 130.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.56, 49.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.650 CPU sec, 4.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13155, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.01, 50.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.670 CPU sec, 5.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13192, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (131.36, 51.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.187 CPU sec, 5.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12986, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.86, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.013 CPU sec, 6.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12915, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.83, 52.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.934 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13073, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.13, 49.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.796 CPU sec, 6.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13047, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 69, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (119.00, 86.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.84, 50.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.565 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12991, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (125.69, 65.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (130.84, 50.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.152 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13176, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.29, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.332 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13015, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.04, 49.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.641 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13005, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (130.04, 49.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.942 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13091, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.90, 50.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.298 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12984, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (133.25, 52.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.035 CPU sec, 6.799 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12965, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (15, 113, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (100.20, 86.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.92, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.089 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13015, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 99, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (100.70, 67.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.04, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.196 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13102, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.73, 51.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.457 CPU sec, 5.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13012, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.99, 50.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.369 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12872, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 77, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (102.70, 69.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.52, 52.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.150 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12954, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 149, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (117.33, 67.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.47, 50.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.169 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12996, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 62, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (68.64, 30.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (129.88, 49.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.020 CPU sec, 4.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12908, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.10, 48.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.433 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12817, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.58, 50.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.791 CPU sec, 5.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13144, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 122, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (152.54, 134.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.64, 53.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.419 CPU sec, 6.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13116, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 116, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (132.00, 51.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (131.94, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.525 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13148, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 125, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (148.45, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.83, 51.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.831 CPU sec, 5.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12985, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 99, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (125.35, 81.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.76, 52.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.675 CPU sec, 5.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12928, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (147.33, 108.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (131.47, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.621 CPU sec, 6.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 12970, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (134.15, 53.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.468 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13178, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.89, 52.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.247 CPU sec, 5.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12979, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.43, 49.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.953 CPU sec, 4.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12720, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (134.11, 52.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (131.39, 50.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.105 CPU sec, 5.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12969, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.59, 52.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.806 CPU sec, 5.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13073, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 186, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (167.45, 100.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.06, 52.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.259 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12995, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.59, 51.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.443 CPU sec, 5.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12922, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 73, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (125.91, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.36, 51.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.557 CPU sec, 5.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13091, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 106, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (99.60, 68.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (133.10, 53.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.302 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 13132, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.30, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.210 CPU sec, 6.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13063, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 77, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (88.75, 33.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.96, 52.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.106 CPU sec, 6.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13298, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.57, 51.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.287 CPU sec, 5.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12874, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.46, 49.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.597 CPU sec, 5.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12828, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.05, 51.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.940 CPU sec, 5.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13064, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 97, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (86.89, 54.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.38, 52.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.391 CPU sec, 5.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13198, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.29, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.105 CPU sec, 5.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13036, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 89, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (103.08, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.27, 52.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.462 CPU sec, 5.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13044, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 75, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (109.50, 81.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.58, 51.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.965 CPU sec, 5.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13243, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 92, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (136.23, 93.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (133.38, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.730 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13016, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.99, 50.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.514 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13043, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 91, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (124.38, 113.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (129.96, 48.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.676 CPU sec, 5.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13035, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 94, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (110.14, 78.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.96, 48.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.898 CPU sec, 4.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12875, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.83, 47.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.689 CPU sec, 6.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12794, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.14, 50.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.618 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13010, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.79, 52.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.946 CPU sec, 5.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12881, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.73, 51.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.420 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12850, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 149, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (134.18, 54.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.88, 50.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.960 CPU sec, 5.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13062, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 90, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (96.36, 44.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.19, 51.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.525 CPU sec, 5.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12966, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 65, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (68.00, 25.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.67, 51.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.218 CPU sec, 5.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13227, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (64.67, 58.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.33, 49.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.897 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13081, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (75.56, 38.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (130.14, 49.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.332 CPU sec, 4.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12748, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.66, 48.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.665 CPU sec, 5.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12968, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.18, 51.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.911 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12946, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (132.39, 51.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.290 CPU sec, 6.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13023, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (130.76, 51.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.243 CPU sec, 5.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12979, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.59, 52.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.867 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12968, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.16, 50.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.782 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12974, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (132.53, 52.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.782 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13243, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.18, 50.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.080 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13273, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (130.47, 50.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.849 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12878, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (130.52, 50.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.310 CPU sec, 5.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12923, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 95, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (102.91, 82.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (131.07, 50.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.195 CPU sec, 5.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13086, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 66, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (63.11, 39.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (131.25, 50.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.962 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13051, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (131.46, 50.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.071 CPU sec, 5.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13048, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 121, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (107.40, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (132.34, 52.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.608 CPU sec, 5.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13018, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.26, 51.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.376 CPU sec, 5.807 real sec [M::process] read 46980 sequences (7093980 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13031, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 103, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (130.12, 93.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 127, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (132.16, 51.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.172 CPU sec, 4.833 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9285, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.43, 50.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 46980 reads in 107.237 CPU sec, 4.329 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21156\tSM:UG10S2P090\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21156_HLKNYBGXX_UG10S2P090_R1.fastq.gz 5347_7332_21156_HLKNYBGXX_UG10S2P090_R2.fastq.gz [main] Real time: 1292.185 sec; CPU: 33697.507 sec Finished alignement of UG10S2P090 at Wed Apr 3 03:50:16 2019 Sorting BAM file started at Wed Apr 3 03:50:16 2019 samtools sort -@ 30 -m 4G UG10S2P090_unsorted.bam -o UG10S2P090.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 03:50:16 2019 rm UG10S2P090_unsorted.bam rm 5347_7332_21156_HLKNYBGXX_UG10S2P090_R1.fastq.gz rm 5347_7332_21156_HLKNYBGXX_UG10S2P090_R2.fastq.gz samtools index UG10S2P090.bam Finished BAM file generation of the sample - UG10S2P090 Processing fastq files for the sample - UG10S1P014 Reading 5347_7332_21157_HLKNYBGXX_UG10S1P014_R1.fastq.gz 5347_7332_21157_HLKNYBGXX_UG10S1P014_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21157\tSM:UG10S1P014\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21157_HLKNYBGXX_UG10S1P014_R1.fastq.gz 5347_7332_21157_HLKNYBGXX_UG10S1P014_R2.fastq.gz | samtools view -Sb1 - > UG10S1P014_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11223, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 85, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (88.75, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (145.86, 56.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.069 CPU sec, 7.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11499, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 111, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (110.00, 55.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (149.90, 60.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.979 CPU sec, 7.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11412, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.27, 58.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 184.180 CPU sec, 6.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11621, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (128.25, 76.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (146.40, 57.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.836 CPU sec, 6.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11445, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.37, 57.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 186.712 CPU sec, 6.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11468, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 93, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (112.45, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.16, 58.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.141 CPU sec, 6.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11555, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 82, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (83.10, 42.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (147.95, 57.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.876 CPU sec, 7.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11382, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (145.77, 56.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.950 CPU sec, 6.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11420, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (146.99, 57.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.838 CPU sec, 6.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11306, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (148.61, 59.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 194.400 CPU sec, 6.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11357, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 142, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (129.20, 68.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (148.51, 59.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.378 CPU sec, 6.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11552, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 93, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (124.18, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.56, 58.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.718 CPU sec, 13.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11498, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (145.09, 55.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 180.482 CPU sec, 7.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11317, 2, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (147.04, 58.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.023 CPU sec, 6.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11374, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (145.63, 55.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.313 CPU sec, 6.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11390, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (146.69, 57.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.784 CPU sec, 7.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11494, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (146.61, 56.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.560 CPU sec, 6.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11258, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 189, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (195.18, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (147.76, 57.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.583 CPU sec, 7.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11367, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 109, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (139.00, 132.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (149.49, 60.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.164 CPU sec, 7.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11267, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 106, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (109.00, 44.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.34, 58.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.063 CPU sec, 7.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11632, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (146.84, 56.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 186.145 CPU sec, 6.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11330, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 122, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (128.80, 62.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (147.39, 57.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.490 CPU sec, 6.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11454, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (150.32, 60.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 193.269 CPU sec, 6.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11421, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (120.07, 58.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (148.07, 58.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.915 CPU sec, 7.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11352, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (146.29, 55.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.014 CPU sec, 6.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11403, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 82, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (109.69, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (147.57, 58.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.092 CPU sec, 6.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11463, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 122, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (153.42, 150.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (147.98, 58.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.637 CPU sec, 7.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11338, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 94, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (125.90, 78.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (147.77, 58.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.415 CPU sec, 6.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11373, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (147.59, 58.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 198.125 CPU sec, 7.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11283, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 93, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (103.44, 69.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (145.05, 55.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.512 CPU sec, 6.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11400, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 94, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (130.33, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (149.49, 60.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.711 CPU sec, 7.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11453, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (147.65, 57.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.038 CPU sec, 6.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11410, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.54, 58.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 203.336 CPU sec, 7.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11243, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (146.16, 56.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 189.213 CPU sec, 6.674 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11200, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.07, 58.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 186.242 CPU sec, 6.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11284, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 156, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (176.92, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (147.84, 58.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.031 CPU sec, 7.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11344, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 112, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (97.00, 41.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (146.13, 56.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.177 CPU sec, 6.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11524, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 77, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (83.60, 59.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (146.24, 56.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.053 CPU sec, 6.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11261, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (148.78, 59.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.158 CPU sec, 6.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11348, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 92, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (90.50, 20.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.12, 57.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.462 CPU sec, 7.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11354, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (135.17, 38.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (148.69, 59.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.231 CPU sec, 7.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11600, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 89, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (113.17, 59.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (147.57, 57.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.112 CPU sec, 7.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11169, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (146.39, 57.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.517 CPU sec, 6.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11288, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (148.63, 58.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.105 CPU sec, 6.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11406, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 134, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (113.22, 40.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (149.35, 59.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.480 CPU sec, 7.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11391, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.78, 58.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.286 CPU sec, 6.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11196, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (114.88, 33.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (145.22, 55.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.096 CPU sec, 6.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11304, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (149.54, 60.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 192.885 CPU sec, 6.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11410, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.15, 59.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.327 CPU sec, 6.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11332, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (146.94, 57.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.957 CPU sec, 7.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11239, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 130, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (149.67, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 137, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (144.23, 55.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.950 CPU sec, 6.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11405, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 109, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (102.82, 34.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.40, 58.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.167 CPU sec, 6.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11404, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 116, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (101.20, 34.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.55, 57.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.081 CPU sec, 7.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11369, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (147.32, 57.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 187.165 CPU sec, 6.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11292, 1, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (149.18, 59.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.398 CPU sec, 6.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11474, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 129, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (139.58, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (145.74, 55.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.156 CPU sec, 6.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11456, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (148.57, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 187.394 CPU sec, 6.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11474, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (147.66, 57.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 178.447 CPU sec, 6.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11207, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (146.86, 56.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 196.172 CPU sec, 7.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11493, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 147, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (134.18, 63.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (146.75, 56.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.910 CPU sec, 7.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11439, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (147.02, 58.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 192.315 CPU sec, 7.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11485, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (148.88, 58.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 193.569 CPU sec, 6.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11383, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 81, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (63.50, 26.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (148.09, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.694 CPU sec, 6.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11500, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 103, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (81.25, 37.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.49, 57.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.271 CPU sec, 6.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11380, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (87.90, 30.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.39, 57.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.721 CPU sec, 6.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11310, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (147.27, 57.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 200.830 CPU sec, 7.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 11305, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (147.54, 57.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 178.655 CPU sec, 6.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11552, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 92, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (82.70, 56.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (148.30, 58.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.069 CPU sec, 7.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11561, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (147.51, 57.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 183.304 CPU sec, 7.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11453, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 151, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (137.50, 74.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (148.25, 58.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.658 CPU sec, 7.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11289, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 177, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (179.00, 106.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (147.67, 58.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.368 CPU sec, 6.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11611, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (146.05, 56.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 197.388 CPU sec, 7.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11598, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (145.71, 56.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 177.261 CPU sec, 7.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11531, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (91.67, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (147.16, 56.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.357 CPU sec, 7.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11528, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (147.58, 57.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.493 CPU sec, 7.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11312, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.96, 58.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 199.748 CPU sec, 7.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11322, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (145.11, 55.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.298 CPU sec, 6.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11267, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (147.56, 57.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 187.017 CPU sec, 6.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11481, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (148.56, 59.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 186.065 CPU sec, 6.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11295, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (148.29, 58.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.099 CPU sec, 7.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11449, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 98, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (122.84, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (146.18, 56.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.291 CPU sec, 6.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11214, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (146.86, 56.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 197.542 CPU sec, 7.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11214, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 111, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (112.60, 50.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (149.17, 59.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.122 CPU sec, 6.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11502, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 100, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (146.62, 118.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (147.75, 58.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.983 CPU sec, 7.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11521, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (96.70, 49.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (146.06, 56.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.883 CPU sec, 6.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11221, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 131, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (137.92, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (145.09, 54.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.986 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11542, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 74, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (92.22, 62.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (146.47, 56.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.511 CPU sec, 6.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11363, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (149.22, 60.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.939 CPU sec, 6.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11361, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (146.15, 56.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.696 CPU sec, 6.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11282, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (101.00, 54.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 138, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (145.11, 55.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.117 CPU sec, 7.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11552, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (145.84, 55.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 186.965 CPU sec, 6.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11398, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.03, 56.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.247 CPU sec, 6.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11272, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 100, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (96.42, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (147.75, 57.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.531 CPU sec, 7.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11258, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (144.32, 54.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.323 CPU sec, 6.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11508, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 60, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (84.00, 58.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 141, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (150.21, 60.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.558 CPU sec, 6.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11370, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (115.75, 66.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (146.58, 56.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.158 CPU sec, 7.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11521, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 205, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (141.78, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (147.63, 58.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.755 CPU sec, 7.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11359, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (146.16, 55.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.188 CPU sec, 6.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11313, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (148.38, 58.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.299 CPU sec, 6.545 real sec [M::process] read 61326 sequences (9260226 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11553, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (148.38, 58.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.435 CPU sec, 7.085 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10481, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (145.38, 55.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 61326 reads in 166.687 CPU sec, 6.101 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21157\tSM:UG10S1P014\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21157_HLKNYBGXX_UG10S1P014_R1.fastq.gz 5347_7332_21157_HLKNYBGXX_UG10S1P014_R2.fastq.gz [main] Real time: 709.748 sec; CPU: 19195.326 sec Finished alignement of UG10S1P014 at Wed Apr 3 04:12:56 2019 Sorting BAM file started at Wed Apr 3 04:12:56 2019 samtools sort -@ 30 -m 4G UG10S1P014_unsorted.bam -o UG10S1P014.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 04:12:56 2019 rm UG10S1P014_unsorted.bam rm 5347_7332_21157_HLKNYBGXX_UG10S1P014_R1.fastq.gz rm 5347_7332_21157_HLKNYBGXX_UG10S1P014_R2.fastq.gz samtools index UG10S1P014.bam Finished BAM file generation of the sample - UG10S1P014 Processing fastq files for the sample - UG10S2P109 Reading 5347_7332_21158_HLKNYBGXX_UG10S2P109_R1.fastq.gz 5347_7332_21158_HLKNYBGXX_UG10S2P109_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21158\tSM:UG10S2P109\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21158_HLKNYBGXX_UG10S2P109_R1.fastq.gz 5347_7332_21158_HLKNYBGXX_UG10S2P109_R2.fastq.gz | samtools view -Sb1 - > UG10S2P109_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12854, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 147, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (144.50, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (189.23, 92.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.383 CPU sec, 4.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13113, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 94, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (152.94, 111.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (192.40, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.195 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13251, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 124, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (159.60, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (191.28, 94.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.135 CPU sec, 4.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13144, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 102, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (107.53, 44.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (193.76, 98.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.046 CPU sec, 5.089 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13175, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (153.12, 99.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (193.38, 97.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.844 CPU sec, 4.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13223, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 125, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (127.15, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (190.22, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.070 CPU sec, 4.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13023, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (130.62, 49.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (187.52, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.651 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13081, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 84, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (95.19, 62.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (186.08, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.413 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13027, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (166.78, 101.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (187.14, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.292 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13070, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 128, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (131.44, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.12, 89.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.980 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12845, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 169, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (161.94, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.84, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.139 CPU sec, 5.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12983, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 112, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (126.76, 62.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.99, 91.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.227 CPU sec, 5.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13068, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 212, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (172.35, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.18, 94.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.857 CPU sec, 4.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13234, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 65, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (76.08, 43.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (191.47, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.504 CPU sec, 4.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13313, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (137.57, 58.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.30, 94.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.278 CPU sec, 5.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13108, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 135, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 238) [M::mem_pestat] mean and std.dev: (129.90, 39.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (191.03, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.180 CPU sec, 4.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13084, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (126.44, 72.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (188.28, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.043 CPU sec, 4.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13218, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 106, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (118.00, 48.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.47, 91.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.686 CPU sec, 4.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13271, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 145, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (161.38, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.37, 90.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.681 CPU sec, 6.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12966, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 95, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (126.68, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (184.25, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.558 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13003, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (122.44, 69.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (183.90, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.199 CPU sec, 5.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12856, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 131, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (127.80, 51.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (189.13, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.740 CPU sec, 4.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13037, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (145.94, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (192.06, 94.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.986 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13230, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 144, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (143.41, 91.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (188.95, 92.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.723 CPU sec, 3.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13028, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (121.67, 67.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (191.67, 95.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.283 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13068, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 142, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (234.43, 190.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.75, 93.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.965 CPU sec, 4.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13023, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 144, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (154.81, 108.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (188.19, 91.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.839 CPU sec, 4.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13090, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 182, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 322) [M::mem_pestat] mean and std.dev: (158.00, 30.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (187.52, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.485 CPU sec, 4.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12987, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (129.29, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.89, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.047 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12914, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 121, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (119.10, 76.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (186.48, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.044 CPU sec, 6.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13071, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (139.21, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (182.32, 86.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.010 CPU sec, 4.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12847, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 141, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (137.70, 74.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (184.10, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.353 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13122, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 121, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (135.82, 99.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.05, 91.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.488 CPU sec, 4.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13169, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 103, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (146.69, 115.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.85, 94.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.480 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13051, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 164, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (198.75, 121.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (191.11, 96.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.750 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13206, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 259, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (216.27, 121.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (187.01, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.637 CPU sec, 5.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13095, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (143.33, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (187.42, 91.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.739 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13134, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 107, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (117.47, 86.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.16, 91.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.230 CPU sec, 4.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13202, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 204, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (154.75, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (185.18, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.838 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13048, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 108, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (110.50, 58.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.57, 89.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.183 CPU sec, 5.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13103, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 119, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (135.80, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.63, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.435 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12978, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (174.82, 81.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (183.23, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.519 CPU sec, 4.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13039, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 100, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (149.50, 115.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (187.29, 91.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.100 CPU sec, 4.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12898, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (165.64, 93.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (186.25, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.211 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13039, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 142, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (149.71, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (189.25, 93.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.238 CPU sec, 4.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13089, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 143, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (156.75, 109.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (189.50, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.907 CPU sec, 4.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13072, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 132, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (131.80, 55.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (188.22, 92.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.018 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13050, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 105, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (159.55, 112.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (187.08, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.793 CPU sec, 4.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13157, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 88, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (176.58, 171.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (187.69, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.695 CPU sec, 4.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13109, 6, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.89, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.244 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13103, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 123, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (122.40, 77.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (186.03, 89.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.669 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12897, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 98, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (160.23, 146.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (186.73, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.535 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12971, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 115, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (111.11, 48.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (183.29, 87.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.639 CPU sec, 6.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13025, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (140.20, 60.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.66, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.236 CPU sec, 4.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13116, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 92, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (104.50, 41.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (189.77, 93.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.299 CPU sec, 4.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13032, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 95, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (111.39, 53.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (190.40, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.975 CPU sec, 4.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13063, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 132, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (152.06, 58.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (188.29, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.790 CPU sec, 4.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13040, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (114.36, 50.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (187.93, 92.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.083 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13161, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 176, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (229.53, 165.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.58, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.575 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13076, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 62, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (104.85, 87.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (183.56, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.207 CPU sec, 3.747 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13019, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 67, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (100.59, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (184.03, 87.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.101 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12990, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 129, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.25, 47.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (185.79, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.072 CPU sec, 4.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13151, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 103, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (123.15, 69.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (191.99, 95.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.449 CPU sec, 4.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13111, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.78, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.818 CPU sec, 4.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13023, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 173, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (187.42, 132.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (193.32, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.198 CPU sec, 4.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13171, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 73, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (99.80, 58.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (193.99, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.892 CPU sec, 4.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13201, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 149, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (133.33, 81.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (192.07, 96.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.445 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13126, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 116, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (143.25, 92.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (191.40, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.017 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12933, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 82, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (137.65, 98.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (189.78, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.171 CPU sec, 4.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13093, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 79, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (114.91, 63.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.45, 90.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.644 CPU sec, 3.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12826, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 145, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (159.47, 97.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (184.06, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.526 CPU sec, 4.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12724, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (144.46, 67.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.46, 85.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.092 CPU sec, 4.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12887, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 189, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (172.58, 115.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (191.59, 94.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.013 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13226, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (193.59, 96.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.508 CPU sec, 4.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13035, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 162, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (159.08, 74.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (193.58, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.849 CPU sec, 4.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13102, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 108, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (150.20, 113.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (189.99, 93.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.348 CPU sec, 4.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13076, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 151, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (125.00, 54.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (191.51, 96.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.271 CPU sec, 4.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13292, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 102, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (119.58, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (193.21, 95.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.425 CPU sec, 4.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13066, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 132, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (186.09, 106.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (189.50, 94.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.971 CPU sec, 4.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13127, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 110, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (113.21, 42.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.77, 90.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.011 CPU sec, 4.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13041, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 264, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (218.20, 154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.52, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.979 CPU sec, 4.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12799, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 140, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (165.13, 95.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.87, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.300 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12624, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 102, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (130.07, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (185.43, 89.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.890 CPU sec, 4.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13041, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 159, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (169.33, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (192.62, 96.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.143 CPU sec, 5.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13119, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 81, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (140.00, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (193.50, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.836 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13019, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (193.62, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 114.055 CPU sec, 4.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13068, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 98, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (111.40, 58.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.84, 94.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.103 CPU sec, 4.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13158, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 141, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (132.58, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (191.79, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.283 CPU sec, 4.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13042, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 131, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (126.38, 53.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.13, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.601 CPU sec, 4.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13279, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 135, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (145.71, 62.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (188.48, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.601 CPU sec, 4.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13041, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (151.00, 120.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (184.99, 87.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.974 CPU sec, 5.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12840, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 92, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (144.00, 127.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.33, 87.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.726 CPU sec, 4.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12521, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 84, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (152.29, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (185.11, 89.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.801 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12933, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 103, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168) [M::mem_pestat] mean and std.dev: (306.80, 403.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.95, 94.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.610 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13115, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 87, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (129.27, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (188.75, 92.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.640 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13188, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 130, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (129.35, 55.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (189.20, 92.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.600 CPU sec, 4.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13217, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (116.94, 62.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (188.07, 92.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.147 CPU sec, 3.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13230, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 133, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (170.47, 131.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (189.51, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.844 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12945, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (144.71, 74.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (188.40, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.278 CPU sec, 3.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13004, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 107, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (122.81, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.39, 91.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.918 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13067, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 145, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (161.60, 86.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (185.67, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.204 CPU sec, 4.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13068, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 224, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (218.80, 212.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.73, 85.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.909 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12711, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (181.50, 85.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.700 CPU sec, 4.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12984, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 145, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (149.06, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (186.35, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.019 CPU sec, 4.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13133, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 219, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (219.50, 115.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (190.81, 94.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.213 CPU sec, 5.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13114, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 65, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (60.00, 35.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (190.73, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.523 CPU sec, 4.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12991, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 91, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (102.46, 59.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.20, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.511 CPU sec, 4.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13094, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 132, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (198.75, 151.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (191.96, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.107 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13109, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 109, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.87, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (189.90, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.076 CPU sec, 3.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13283, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 117, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (125.86, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.27, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.899 CPU sec, 4.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13106, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 122, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 218) [M::mem_pestat] mean and std.dev: (107.29, 44.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (186.00, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.702 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13000, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 186, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (173.67, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.42, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.496 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12855, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 164, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (182.68, 93.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.41, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.681 CPU sec, 4.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12630, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (153.40, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.32, 87.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.560 CPU sec, 4.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13008, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 129, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (153.27, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.90, 94.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.053 CPU sec, 4.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12961, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 132, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (145.23, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (191.69, 95.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.872 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13095, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 185, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (175.71, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.86, 93.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.261 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13079, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 92, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (99.25, 68.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (191.40, 94.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.113 CPU sec, 4.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12990, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 146, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (136.71, 90.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (190.14, 94.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.165 CPU sec, 4.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13057, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (97.07, 57.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.58, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.061 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12952, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 79, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (106.71, 64.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (187.38, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.694 CPU sec, 3.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13169, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 117, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (133.07, 55.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (184.53, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.933 CPU sec, 5.747 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12851, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 103, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (118.18, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.86, 85.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.558 CPU sec, 4.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12723, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (178.31, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.163 CPU sec, 4.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12982, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 102, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (114.83, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.77, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.570 CPU sec, 4.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13055, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (123.11, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (185.05, 89.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.422 CPU sec, 4.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13157, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.13, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (188.55, 92.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.764 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13057, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 162, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (188.00, 111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (189.69, 92.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.486 CPU sec, 4.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13222, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 166, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (140.69, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (185.89, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.148 CPU sec, 5.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13263, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 107, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (95.40, 47.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (188.66, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.362 CPU sec, 3.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12959, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 127, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (142.44, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.34, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.743 CPU sec, 4.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12920, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 142, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (160.38, 91.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.22, 91.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.514 CPU sec, 4.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13222, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.44, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.790 CPU sec, 4.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13050, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 154, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (168.42, 113.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (187.95, 92.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.438 CPU sec, 4.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13272, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 115, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (116.58, 66.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.48, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.515 CPU sec, 4.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13202, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 151, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (146.88, 74.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (185.09, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.064 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13039, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (138.74, 80.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (186.31, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.134 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13045, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 87, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (67.58, 32.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (192.05, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.045 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13088, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 105, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (155.13, 103.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (191.05, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.470 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13267, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 119, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (117.25, 62.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.23, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.961 CPU sec, 4.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13062, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 106, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (141.77, 99.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (187.67, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.342 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12987, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 116, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (88.25, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.73, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.103 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13153, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (167.56, 81.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (188.41, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.861 CPU sec, 5.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13333, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 125, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (173.36, 111.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (188.04, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.522 CPU sec, 4.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12965, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 144, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (142.33, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (185.18, 88.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.992 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13095, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 129, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (145.19, 78.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.79, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.750 CPU sec, 4.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13069, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 158, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (160.21, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (183.57, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.640 CPU sec, 4.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13015, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 92, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (104.67, 58.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (189.69, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.161 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13185, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 94, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (105.85, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.50, 91.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.355 CPU sec, 4.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13146, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 80, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (118.86, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.78, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.191 CPU sec, 4.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12811, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (187.08, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.718 CPU sec, 4.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12979, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (159.50, 99.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (188.82, 93.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.029 CPU sec, 6.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13200, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 92, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 175) [M::mem_pestat] mean and std.dev: (82.92, 22.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (190.35, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.909 CPU sec, 5.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12976, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (138.08, 89.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.44, 91.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.015 CPU sec, 5.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13103, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (104.91, 40.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.74, 87.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.005 CPU sec, 5.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13095, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (141.20, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (183.34, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.762 CPU sec, 4.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13097, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (90.75, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (184.04, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.226 CPU sec, 4.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13136, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 103, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (96.64, 35.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (187.02, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.776 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13373, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 160, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 304) [M::mem_pestat] mean and std.dev: (143.50, 45.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.96, 91.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.912 CPU sec, 5.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13148, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 95, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (124.33, 86.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.66, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.780 CPU sec, 5.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12916, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 92, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (117.31, 71.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.75, 85.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.486 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13115, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 146, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (127.00, 43.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (184.20, 88.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.164 CPU sec, 4.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13042, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 95, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (100.14, 56.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (184.48, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.118 CPU sec, 4.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13053, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 92, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (101.25, 52.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (189.81, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.622 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13205, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 173, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (177.58, 104.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (183.55, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.913 CPU sec, 4.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13191, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 87, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (110.56, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (184.33, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.905 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12932, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 158, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (162.32, 108.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (184.43, 87.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.112 CPU sec, 4.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13248, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 94, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (102.00, 43.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (186.19, 90.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.181 CPU sec, 5.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13181, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 120, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (146.14, 90.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.20, 91.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.268 CPU sec, 4.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13034, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (136.67, 64.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (185.98, 91.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.369 CPU sec, 4.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12796, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 108, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (116.79, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (183.39, 85.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.481 CPU sec, 4.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13071, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 151, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (140.09, 40.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (183.35, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.142 CPU sec, 4.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13010, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 122, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2070) [M::mem_pestat] mean and std.dev: (235.55, 261.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.70, 89.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.984 CPU sec, 4.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13116, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 117, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (151.40, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (188.49, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.999 CPU sec, 4.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13063, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (141.10, 65.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (183.17, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.320 CPU sec, 4.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13215, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 177, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (171.00, 104.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.77, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.903 CPU sec, 5.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13136, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 135, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (155.28, 108.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.88, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.825 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13055, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 123, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (150.91, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (187.27, 91.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.238 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13079, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 183, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (153.53, 71.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (184.93, 87.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.270 CPU sec, 3.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13138, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 191, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (171.62, 60.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.42, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.439 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12990, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 206) [M::mem_pestat] mean and std.dev: (95.90, 26.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (182.48, 86.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.890 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13042, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 147, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (142.00, 81.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.85, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.483 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13069, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (161.17, 121.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.49, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.063 CPU sec, 4.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13323, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 147, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (164.80, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (184.16, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.168 CPU sec, 3.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12862, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (127.31, 70.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.62, 86.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.726 CPU sec, 4.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12926, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 121, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (162.18, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (181.59, 84.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.089 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12730, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (184.09, 86.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.61, 84.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.463 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13014, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 84, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (116.73, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (189.37, 92.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.653 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13092, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (190.48, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.938 CPU sec, 4.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13141, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 85, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (147.00, 123.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.26, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.351 CPU sec, 4.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12932, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (188.62, 91.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.800 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13267, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (178.19, 137.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (189.55, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.766 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13025, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (167.75, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (193.55, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.674 CPU sec, 4.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13347, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 105, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (131.08, 79.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.63, 91.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.119 CPU sec, 5.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13251, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 103, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (123.56, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.30, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.228 CPU sec, 4.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12885, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 132, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (128.72, 55.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.75, 84.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.226 CPU sec, 5.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12645, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 117, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (92.18, 46.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (183.12, 86.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.864 CPU sec, 4.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13070, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (121.48, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (188.03, 91.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.499 CPU sec, 4.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13136, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 171, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (155.72, 85.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.76, 94.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.880 CPU sec, 4.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13163, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 181, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (199.38, 172.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (188.11, 92.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.728 CPU sec, 4.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13208, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (119.00, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (187.51, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.749 CPU sec, 4.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13111, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 131, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (122.50, 33.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (190.99, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.941 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13300, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 122, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (159.18, 92.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (191.80, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.080 CPU sec, 5.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13196, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 75, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (92.53, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (191.31, 95.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.270 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13102, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (142.69, 66.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (186.36, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.451 CPU sec, 4.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13043, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (144.25, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (185.17, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.635 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12754, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 165, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (166.25, 84.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (184.64, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.815 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12904, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 118, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (100.79, 30.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (188.22, 92.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.541 CPU sec, 4.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13188, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 91, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (89.27, 29.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (189.43, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.670 CPU sec, 4.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13077, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (129.92, 84.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.55, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.119 CPU sec, 4.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13211, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (156.35, 101.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (186.46, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.470 CPU sec, 4.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13201, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (132.31, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (189.59, 93.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.772 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13116, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 115, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (99.36, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (190.34, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.730 CPU sec, 5.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13156, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 66, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (80.75, 45.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (191.12, 94.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.658 CPU sec, 4.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13106, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 133, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (146.15, 107.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.82, 84.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.676 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12934, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 130, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (154.07, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.59, 85.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.985 CPU sec, 4.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12657, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (150.24, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (175.98, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.731 CPU sec, 4.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13175, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 129, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (137.25, 52.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (186.60, 91.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.597 CPU sec, 5.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13018, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 186, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (181.94, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.65, 91.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.628 CPU sec, 4.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13248, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 158, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (148.85, 59.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (185.96, 90.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.741 CPU sec, 5.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13035, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 177, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (159.79, 73.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (189.76, 92.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.659 CPU sec, 6.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13204, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 111, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (128.45, 64.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (188.58, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.032 CPU sec, 4.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13134, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 100, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (114.27, 72.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (188.17, 92.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.429 CPU sec, 4.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13116, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (163.24, 102.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (189.44, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.261 CPU sec, 4.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13014, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 205, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (155.00, 84.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (184.26, 87.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.171 CPU sec, 4.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13132, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (175.41, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.94, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.529 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12611, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 135, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (166.14, 94.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (175.99, 79.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.980 CPU sec, 4.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12980, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 123, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (142.47, 117.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (187.77, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.351 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13079, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 188, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (224.40, 199.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (190.90, 94.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.782 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13114, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 84, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (130.06, 107.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.47, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.526 CPU sec, 4.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13195, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 143, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (138.95, 81.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (183.65, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.934 CPU sec, 5.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13086, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 147, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (144.91, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (186.82, 90.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.287 CPU sec, 4.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13216, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 113, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (123.00, 70.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (188.76, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.565 CPU sec, 4.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13339, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 122, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (121.58, 69.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (191.68, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.278 CPU sec, 4.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13190, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (127.44, 64.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (184.71, 88.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.520 CPU sec, 4.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13062, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (99.80, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.80, 85.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.655 CPU sec, 4.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12634, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (142.33, 63.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (181.45, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.164 CPU sec, 5.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13048, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 109, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (104.27, 52.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (184.75, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.771 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13315, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 143, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (208.40, 180.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (187.70, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.371 CPU sec, 5.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13113, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (148.18, 81.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (185.81, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.167 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13089, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 102, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (103.00, 54.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (185.67, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.742 CPU sec, 3.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12992, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 112, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (132.19, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (188.12, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.687 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12978, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 132, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (140.05, 77.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (189.84, 93.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.862 CPU sec, 4.239 real sec [M::process] read 418 sequences (63118 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13154, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (185.06, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 109.212 CPU sec, 4.138 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 94, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 171, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (181.11, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 418 reads in 1.850 CPU sec, 0.095 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21158\tSM:UG10S2P109\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21158_HLKNYBGXX_UG10S2P109_R1.fastq.gz 5347_7332_21158_HLKNYBGXX_UG10S2P109_R2.fastq.gz [main] Real time: 1155.048 sec; CPU: 28675.915 sec Finished alignement of UG10S2P109 at Wed Apr 3 04:25:16 2019 Sorting BAM file started at Wed Apr 3 04:25:16 2019 samtools sort -@ 30 -m 4G UG10S2P109_unsorted.bam -o UG10S2P109.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 04:25:16 2019 rm UG10S2P109_unsorted.bam rm 5347_7332_21158_HLKNYBGXX_UG10S2P109_R1.fastq.gz rm 5347_7332_21158_HLKNYBGXX_UG10S2P109_R2.fastq.gz samtools index UG10S2P109.bam Finished BAM file generation of the sample - UG10S2P109 Processing fastq files for the sample - UG10S2P110 Reading 5347_7332_21159_HLKNYBGXX_UG10S2P110_R1.fastq.gz 5347_7332_21159_HLKNYBGXX_UG10S2P110_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21159\tSM:UG10S2P110\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21159_HLKNYBGXX_UG10S2P110_R1.fastq.gz 5347_7332_21159_HLKNYBGXX_UG10S2P110_R2.fastq.gz | samtools view -Sb1 - > UG10S2P110_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11427, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (129.98, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 244.825 CPU sec, 8.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11383, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.05, 52.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 235.276 CPU sec, 8.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11257, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (131.41, 54.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 228.258 CPU sec, 8.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10796, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.51, 53.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 254.587 CPU sec, 8.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10896, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 83, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (79.90, 34.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (130.12, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.774 CPU sec, 9.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11084, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 95, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (107.87, 50.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.17, 54.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.607 CPU sec, 9.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11711, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.76, 52.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 189.393 CPU sec, 7.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11826, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.67, 52.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.253 CPU sec, 6.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11562, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (129.14, 51.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.444 CPU sec, 8.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11272, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (130.76, 52.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 248.254 CPU sec, 8.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11435, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (131.97, 54.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 235.210 CPU sec, 8.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11151, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (131.71, 53.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 231.052 CPU sec, 8.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 10950, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 93, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (120.83, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.46, 54.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.930 CPU sec, 8.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11236, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (132.11, 54.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 234.788 CPU sec, 8.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11794, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 175, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (204.07, 184.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.56, 51.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.702 CPU sec, 7.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11809, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.60, 50.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.689 CPU sec, 7.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11741, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 147, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (139.80, 67.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (127.95, 50.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.803 CPU sec, 7.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11297, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (131.00, 53.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 223.703 CPU sec, 7.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11355, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 173, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (283.09, 443.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (129.89, 52.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.930 CPU sec, 9.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11202, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 75, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (79.82, 52.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.52, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.435 CPU sec, 8.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 10953, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (128.63, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 274.795 CPU sec, 9.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11077, 2, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.46, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 244.932 CPU sec, 8.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11413, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.29, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 221.591 CPU sec, 7.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11690, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 140, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (145.69, 87.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (128.71, 50.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.104 CPU sec, 6.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11736, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (128.75, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 187.938 CPU sec, 6.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11689, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.14, 51.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 216.389 CPU sec, 8.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11334, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.91, 51.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 222.685 CPU sec, 8.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11417, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (130.93, 54.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 226.543 CPU sec, 8.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11069, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.22, 53.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 238.422 CPU sec, 9.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10999, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 78, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (88.46, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.97, 51.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.348 CPU sec, 8.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11195, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (129.00, 51.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.443 CPU sec, 7.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11624, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 96, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (100.67, 54.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.88, 51.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.115 CPU sec, 7.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11825, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 155, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (144.00, 84.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.06, 52.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.210 CPU sec, 7.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11656, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 91, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (118.27, 96.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.98, 52.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.365 CPU sec, 6.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11338, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 68, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (61.89, 25.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.06, 51.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.956 CPU sec, 8.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11354, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 120, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (112.44, 58.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.55, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.113 CPU sec, 8.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10921, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (131.08, 53.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 249.574 CPU sec, 9.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10902, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 110, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (100.50, 52.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.61, 52.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.198 CPU sec, 9.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10925, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (130.73, 52.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 240.431 CPU sec, 8.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11445, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 82, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (108.29, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (130.34, 51.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.146 CPU sec, 8.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11697, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (108.89, 66.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (128.35, 49.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.284 CPU sec, 7.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11614, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (140.17, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.09, 51.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.590 CPU sec, 7.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11345, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (127.92, 50.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 228.576 CPU sec, 8.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11446, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 74, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (104.60, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (131.06, 54.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.041 CPU sec, 8.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10987, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 85, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (124.56, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.44, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.340 CPU sec, 8.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10775, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (131.15, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (129.98, 52.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 277.995 CPU sec, 9.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10738, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 83, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (74.36, 31.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.70, 52.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.467 CPU sec, 9.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 10788, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.29, 50.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 249.668 CPU sec, 8.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11553, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.40, 50.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 206.700 CPU sec, 7.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11773, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (128.34, 51.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 199.530 CPU sec, 7.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11434, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 150, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (127.36, 80.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.29, 52.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.427 CPU sec, 7.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11487, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 144, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (147.00, 87.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.77, 52.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.848 CPU sec, 7.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11340, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (130.39, 53.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 219.889 CPU sec, 7.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11093, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (111.25, 57.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (132.08, 54.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.116 CPU sec, 8.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10928, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.42, 52.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 255.720 CPU sec, 8.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11282, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (131.18, 54.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 224.230 CPU sec, 8.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11616, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 131, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (152.92, 106.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.78, 52.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.823 CPU sec, 7.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11947, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 67, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (85.18, 54.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (130.00, 53.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.236 CPU sec, 7.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11505, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 262, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (276.83, 208.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.63, 49.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.603 CPU sec, 6.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11375, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (130.44, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 216.572 CPU sec, 7.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11325, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 86, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (85.15, 38.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (131.61, 54.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.465 CPU sec, 8.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11020, 1, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 126, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (132.25, 54.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 242.330 CPU sec, 8.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 10903, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (132.40, 54.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 248.971 CPU sec, 8.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11049, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 115, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (99.31, 53.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (130.90, 53.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.875 CPU sec, 8.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11295, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.80, 53.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 232.206 CPU sec, 8.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11712, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.32, 50.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 199.936 CPU sec, 7.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11601, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (122.08, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.52, 50.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.420 CPU sec, 6.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11216, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (133.80, 81.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (129.90, 52.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.658 CPU sec, 7.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11066, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 110, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (96.62, 71.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (132.41, 55.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.773 CPU sec, 7.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11092, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 126, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (110.38, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (132.18, 54.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.411 CPU sec, 8.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11005, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.59, 54.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 253.171 CPU sec, 8.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10870, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (132.11, 55.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 244.066 CPU sec, 8.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11024, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 162, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (137.25, 65.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (130.16, 52.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.919 CPU sec, 8.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11516, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 110, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (114.27, 60.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.47, 52.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.343 CPU sec, 7.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11863, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 104, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (101.62, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.03, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.955 CPU sec, 6.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11392, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 122, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (128.12, 50.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 192.580 CPU sec, 6.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11283, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (130.31, 52.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 203.205 CPU sec, 7.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11381, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (130.06, 52.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 230.879 CPU sec, 8.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11271, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 121, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (148.82, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.66, 52.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.406 CPU sec, 8.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 11145, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (131.21, 53.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 243.916 CPU sec, 8.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11288, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 161, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (135.22, 61.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.93, 52.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.891 CPU sec, 7.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11452, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 121, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (122.39, 76.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.11, 52.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.324 CPU sec, 7.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11769, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (128.33, 51.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.062 CPU sec, 6.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11728, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.33, 50.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.867 CPU sec, 7.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11433, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (92.00, 31.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.74, 52.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.619 CPU sec, 7.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11224, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 92, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (81.80, 29.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.74, 53.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.635 CPU sec, 7.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11302, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 98, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (122.54, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.80, 52.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.729 CPU sec, 9.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11280, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (158.26, 105.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (132.03, 55.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.315 CPU sec, 9.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11376, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 80, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (117.67, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.60, 51.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.911 CPU sec, 8.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11449, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (148.64, 76.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.17, 55.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.912 CPU sec, 8.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11756, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.74, 51.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.447 CPU sec, 6.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11824, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (127.37, 49.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.532 CPU sec, 5.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11198, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 111, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (154.91, 129.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.34, 50.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.030 CPU sec, 7.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11291, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 93, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (100.54, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (130.17, 52.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.496 CPU sec, 7.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 10974, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (131.27, 53.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 256.841 CPU sec, 8.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10797, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 76, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (127.18, 98.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (131.06, 53.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.569 CPU sec, 9.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10787, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (133.22, 73.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.71, 51.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.753 CPU sec, 8.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10845, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (128.64, 77.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (130.68, 53.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.642 CPU sec, 9.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11328, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.19, 52.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 209.443 CPU sec, 7.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 11547, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.38, 50.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.904 CPU sec, 7.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11497, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 121, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (127.71, 50.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 202.172 CPU sec, 8.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11775, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (123.89, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 122, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (127.81, 50.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.525 CPU sec, 7.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11779, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 147, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (136.25, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.34, 51.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.840 CPU sec, 7.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11269, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (130.40, 111.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.60, 51.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.588 CPU sec, 9.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11059, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 101, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (110.78, 61.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.27, 54.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.240 CPU sec, 9.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11177, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 71, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (77.60, 35.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.78, 52.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.990 CPU sec, 8.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11397, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 255, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (225.45, 162.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.43, 52.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.373 CPU sec, 8.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11366, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.57, 53.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 240.704 CPU sec, 8.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11170, 4, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (130.54, 53.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 240.811 CPU sec, 8.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11590, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 91, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (95.91, 75.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.84, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.278 CPU sec, 7.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11841, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.06, 51.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 192.393 CPU sec, 6.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11374, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.81, 54.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 227.318 CPU sec, 8.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11092, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (130.78, 53.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 269.695 CPU sec, 9.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11297, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.46, 52.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 230.853 CPU sec, 8.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 11474, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.15, 52.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 220.455 CPU sec, 7.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11486, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 103, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (146.20, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.05, 52.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.795 CPU sec, 8.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11256, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.08, 52.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 230.104 CPU sec, 8.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11682, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.15, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 226.126 CPU sec, 8.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12099, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.85, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 178.981 CPU sec, 6.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11692, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 95, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (102.73, 42.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.71, 51.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.456 CPU sec, 6.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11189, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 156, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (208.19, 154.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (132.02, 54.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.213 CPU sec, 7.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11297, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 66, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (58.60, 30.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.96, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.332 CPU sec, 7.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11600, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.84, 51.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 208.010 CPU sec, 7.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11414, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.00, 53.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 243.114 CPU sec, 8.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11473, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.97, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 251.837 CPU sec, 9.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11366, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 113, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (118.64, 48.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (131.27, 54.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.470 CPU sec, 7.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11656, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 79, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (87.57, 42.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.68, 50.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.830 CPU sec, 7.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11586, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.29, 51.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 222.748 CPU sec, 8.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 10844, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (130.01, 51.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 300.042 CPU sec, 11.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10915, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 112, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (118.91, 37.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.88, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 282.156 CPU sec, 9.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11079, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 101, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (131.00, 98.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.78, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.782 CPU sec, 8.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11327, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.60, 50.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 234.702 CPU sec, 8.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11285, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.42, 51.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 260.864 CPU sec, 9.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11324, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (151.25, 58.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.13, 50.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.081 CPU sec, 8.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11732, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (102.11, 49.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.91, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.841 CPU sec, 7.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11642, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (130.23, 52.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.971 CPU sec, 6.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 11084, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.72, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 250.350 CPU sec, 8.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 10974, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.24, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 292.268 CPU sec, 10.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11239, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 207, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (177.25, 108.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (130.16, 53.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.742 CPU sec, 8.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11623, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.38, 50.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 212.595 CPU sec, 7.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 11218, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.83, 51.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 230.637 CPU sec, 8.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11197, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.77, 52.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 259.602 CPU sec, 9.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11809, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 77, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (141.33, 130.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (129.85, 51.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.064 CPU sec, 7.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11648, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 89, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (85.00, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (128.31, 50.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.978 CPU sec, 7.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11306, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.31, 51.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 220.360 CPU sec, 7.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11198, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 179, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (164.27, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.29, 51.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.148 CPU sec, 8.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11233, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 72, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (90.31, 56.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.32, 51.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.014 CPU sec, 8.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 11305, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.78, 50.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 221.403 CPU sec, 7.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11266, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (128.81, 50.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.508 CPU sec, 8.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11559, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.22, 53.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 238.134 CPU sec, 8.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11631, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 110, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (127.00, 60.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.93, 51.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.680 CPU sec, 7.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11684, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.97, 52.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.156 CPU sec, 6.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11311, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 107, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (113.77, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.34, 51.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.105 CPU sec, 7.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11173, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (130.75, 51.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 230.789 CPU sec, 8.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11307, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.47, 52.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 226.045 CPU sec, 8.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11405, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 83, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (67.56, 33.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.44, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.637 CPU sec, 8.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11362, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 123, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (120.53, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (129.31, 51.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.352 CPU sec, 7.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11277, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 156, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (198.31, 140.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.52, 52.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.249 CPU sec, 9.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11175, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 138, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (147.77, 110.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (131.18, 54.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.119 CPU sec, 8.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11637, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (160.08, 102.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (128.87, 51.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.037 CPU sec, 6.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11573, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (130.45, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 193.817 CPU sec, 7.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11017, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (131.93, 55.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 239.077 CPU sec, 8.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11142, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (129.69, 51.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 246.360 CPU sec, 9.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11231, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 70, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (68.90, 45.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.55, 51.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.623 CPU sec, 8.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 10961, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (132.08, 55.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 237.754 CPU sec, 8.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11006, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 137, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (151.91, 110.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (130.87, 53.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.907 CPU sec, 8.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11148, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 133, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (129.44, 102.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (131.12, 53.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.607 CPU sec, 9.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11617, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (128.52, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 204.915 CPU sec, 7.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11607, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.31, 50.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.071 CPU sec, 6.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11575, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 122, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (125.17, 81.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.99, 52.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.036 CPU sec, 7.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11428, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 104, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (133.85, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.34, 53.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.423 CPU sec, 7.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11480, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.90, 53.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.214 CPU sec, 9.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11603, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (130.56, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 215.231 CPU sec, 7.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11701, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 72, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (70.42, 37.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (132.20, 54.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.745 CPU sec, 6.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11583, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (131.19, 53.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 211.635 CPU sec, 7.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11720, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (29, 122, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (130.20, 107.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (128.80, 50.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.726 CPU sec, 7.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11357, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (128.77, 50.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.821 CPU sec, 6.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11139, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (127.91, 50.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 250.777 CPU sec, 8.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 10745, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (129.68, 53.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 253.375 CPU sec, 8.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11013, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.45, 51.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 243.053 CPU sec, 8.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 11254, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (129.90, 52.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 219.431 CPU sec, 8.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11272, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (129.46, 50.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 223.964 CPU sec, 8.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10819, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (131.06, 53.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 262.656 CPU sec, 9.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11839, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 137, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (154.75, 117.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.61, 52.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.253 CPU sec, 7.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11618, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 122, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (127.34, 49.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 195.620 CPU sec, 7.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11256, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (127.85, 49.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 216.824 CPU sec, 8.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11150, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.38, 52.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 259.752 CPU sec, 9.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 11107, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.65, 52.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 237.119 CPU sec, 8.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 11279, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (129.86, 52.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 233.189 CPU sec, 8.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11113, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (129.39, 50.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 237.587 CPU sec, 8.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10850, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 111, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (131.25, 78.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (130.81, 53.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.998 CPU sec, 8.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11379, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (128.96, 51.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 221.700 CPU sec, 7.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11656, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 176, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (167.00, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (129.09, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.496 CPU sec, 7.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11577, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (127.46, 49.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 192.237 CPU sec, 6.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11634, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (113.18, 46.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (130.34, 51.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.081 CPU sec, 7.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11577, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 91, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (105.00, 52.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (130.00, 51.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.712 CPU sec, 8.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11558, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 85, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (82.67, 40.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (129.63, 52.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.549 CPU sec, 7.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11784, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.27, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 207.707 CPU sec, 7.688 real sec [M::process] read 22546 sequences (3404446 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11519, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 77, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (88.44, 52.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (130.91, 52.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.374 CPU sec, 7.000 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 3935, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (131.38, 54.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 22546 reads in 65.700 CPU sec, 2.635 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21159\tSM:UG10S2P110\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21159_HLKNYBGXX_UG10S2P110_R1.fastq.gz 5347_7332_21159_HLKNYBGXX_UG10S2P110_R2.fastq.gz [main] Real time: 1620.910 sec; CPU: 44751.771 sec Finished alignement of UG10S2P110 at Wed Apr 3 04:45:51 2019 Sorting BAM file started at Wed Apr 3 04:45:51 2019 samtools sort -@ 30 -m 4G UG10S2P110_unsorted.bam -o UG10S2P110.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 04:45:51 2019 rm UG10S2P110_unsorted.bam rm 5347_7332_21159_HLKNYBGXX_UG10S2P110_R1.fastq.gz rm 5347_7332_21159_HLKNYBGXX_UG10S2P110_R2.fastq.gz samtools index UG10S2P110.bam Finished BAM file generation of the sample - UG10S2P110 Processing fastq files for the sample - UG10S2P111 Reading 5347_7332_21160_HLKNYBGXX_UG10S2P111_R1.fastq.gz 5347_7332_21160_HLKNYBGXX_UG10S2P111_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21160\tSM:UG10S2P111\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21160_HLKNYBGXX_UG10S2P111_R1.fastq.gz 5347_7332_21160_HLKNYBGXX_UG10S2P111_R2.fastq.gz | samtools view -Sb1 - > UG10S2P111_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12896, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (139.36, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.17, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 284.898 CPU sec, 10.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12971, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 128, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (111.39, 55.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.17, 96.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.855 CPU sec, 9.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13155, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 149, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (157.71, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (191.24, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 269.752 CPU sec, 10.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12915, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 120, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (155.42, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (193.04, 100.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.461 CPU sec, 10.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12700, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 91, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (114.69, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.34, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 267.478 CPU sec, 9.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12487, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 109, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (136.36, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (190.12, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 283.860 CPU sec, 9.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12552, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (146.05, 101.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (186.75, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 277.733 CPU sec, 10.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12711, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 142, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (161.24, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (186.14, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.565 CPU sec, 10.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13363, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 112, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (140.58, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (187.64, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.870 CPU sec, 8.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13228, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (116.90, 80.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.70, 94.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.127 CPU sec, 7.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13323, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (104.64, 55.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.60, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.474 CPU sec, 7.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 12667, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 105, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (126.74, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (187.78, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 273.333 CPU sec, 11.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12803, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 119, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (122.87, 57.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.75, 96.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 277.560 CPU sec, 10.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12950, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 137, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (149.48, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (188.87, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 267.053 CPU sec, 9.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13064, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 107, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (121.41, 78.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (193.18, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.472 CPU sec, 9.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12843, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 145, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (127.38, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.62, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.609 CPU sec, 9.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12768, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 122, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (116.68, 52.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.35, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 284.062 CPU sec, 10.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12874, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 124, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (174.40, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.12, 96.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 278.765 CPU sec, 9.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12935, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (130.38, 76.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.07, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.517 CPU sec, 9.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13215, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 131, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (152.25, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.42, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.323 CPU sec, 8.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13150, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (117.93, 78.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (184.28, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.450 CPU sec, 8.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13249, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 136, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (124.14, 66.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.85, 91.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.489 CPU sec, 7.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13004, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 102, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (109.53, 69.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (185.72, 94.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.082 CPU sec, 8.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12795, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.52, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.54, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.612 CPU sec, 9.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12948, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (137.67, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.43, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.057 CPU sec, 8.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12984, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (109.15, 68.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 169, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (190.86, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 257.958 CPU sec, 8.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12895, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 117, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (138.55, 106.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.19, 96.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.717 CPU sec, 9.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12707, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (119.84, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.49, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.310 CPU sec, 10.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12919, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (128.95, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (189.06, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.812 CPU sec, 9.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12890, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 122, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (105.47, 45.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.48, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 264.412 CPU sec, 9.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13184, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (134.39, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.80, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.138 CPU sec, 8.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 13219, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (91.70, 51.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.62, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.247 CPU sec, 9.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13276, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (148.19, 100.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (187.51, 94.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.136 CPU sec, 8.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12936, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 120, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (138.34, 71.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (186.98, 94.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.609 CPU sec, 8.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13025, 0, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 131, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (136.56, 79.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (185.16, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.107 CPU sec, 9.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 12859, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 140, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (164.63, 109.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.86, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.561 CPU sec, 9.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12849, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (118.32, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.38, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.769 CPU sec, 9.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12753, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 123, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (174.88, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.85, 96.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 262.270 CPU sec, 9.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12597, 9, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (120.39, 72.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (189.79, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.466 CPU sec, 9.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12604, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 132, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (159.39, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.81, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 282.933 CPU sec, 9.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 12616, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 134, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (155.66, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (187.05, 95.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 283.129 CPU sec, 9.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12926, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 126, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (136.05, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.65, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.087 CPU sec, 9.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13208, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 135, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (136.90, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.36, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.247 CPU sec, 8.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13212, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (127.53, 67.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.52, 93.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.683 CPU sec, 8.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13050, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (141.55, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (184.88, 92.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.057 CPU sec, 8.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12903, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 110, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (153.11, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (181.79, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.096 CPU sec, 9.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12887, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (148.26, 102.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.11, 93.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 284.499 CPU sec, 10.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12832, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (123.75, 74.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.20, 94.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.155 CPU sec, 9.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12661, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (116.61, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (188.48, 97.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 276.104 CPU sec, 9.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12623, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (139.04, 62.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (190.27, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 276.231 CPU sec, 9.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12676, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 85, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (117.90, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (186.84, 95.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 304.160 CPU sec, 15.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12714, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 102, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (105.70, 45.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.80, 95.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.992 CPU sec, 10.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13063, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 124, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (145.12, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (185.46, 93.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.986 CPU sec, 9.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13387, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 150, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (168.16, 121.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (185.23, 92.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.991 CPU sec, 9.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13098, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 153, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (148.33, 84.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (186.49, 94.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.744 CPU sec, 9.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13259, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 115, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (114.40, 61.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (185.06, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.045 CPU sec, 8.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12822, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (124.55, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (182.49, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.405 CPU sec, 9.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12851, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (163.12, 102.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (185.67, 94.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.459 CPU sec, 9.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12577, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (122.09, 55.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (187.05, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.315 CPU sec, 9.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12508, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 118, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (141.06, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (185.92, 94.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 277.236 CPU sec, 9.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12372, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 131, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (133.94, 71.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.88, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 285.542 CPU sec, 9.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12420, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (140.55, 81.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.64, 95.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 311.188 CPU sec, 10.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12589, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (158.70, 85.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.64, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.768 CPU sec, 9.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12564, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (131.36, 83.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.73, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 283.512 CPU sec, 9.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13193, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 120, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (157.79, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (185.88, 93.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.271 CPU sec, 8.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13108, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (121.82, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (182.28, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.471 CPU sec, 8.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 13157, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 98, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (124.35, 74.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.24, 94.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.045 CPU sec, 8.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12825, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (159.42, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.54, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.759 CPU sec, 8.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12768, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 133, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (132.50, 72.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.02, 95.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.774 CPU sec, 8.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12785, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 175, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (157.50, 98.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.78, 96.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.142 CPU sec, 9.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12769, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 149, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (149.15, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.63, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.825 CPU sec, 10.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12885, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (106.30, 57.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (190.55, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 282.442 CPU sec, 9.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12805, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 83, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (99.21, 55.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.47, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.281 CPU sec, 9.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12968, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 151, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (162.87, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (191.65, 99.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.130 CPU sec, 9.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12859, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 133, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (156.06, 104.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.83, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 264.490 CPU sec, 9.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 13247, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (136.59, 79.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (183.99, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.857 CPU sec, 9.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13263, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (124.39, 58.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.24, 90.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.400 CPU sec, 8.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 13120, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (132.53, 83.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (183.57, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.955 CPU sec, 8.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12683, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 95, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (102.18, 49.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.24, 94.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.605 CPU sec, 9.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12829, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 108, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (118.81, 62.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (195.71, 103.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.463 CPU sec, 8.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12697, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (113.26, 64.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (191.78, 99.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.171 CPU sec, 8.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 12924, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (145.82, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.06, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 271.752 CPU sec, 9.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12730, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 94, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (107.73, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.26, 100.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.843 CPU sec, 9.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12801, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (143.71, 96.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (190.81, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.463 CPU sec, 9.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12850, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 100, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (105.69, 62.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (190.56, 98.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.604 CPU sec, 8.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13026, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (132.29, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.41, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 262.978 CPU sec, 9.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13171, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (120.10, 63.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.78, 93.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.405 CPU sec, 8.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13052, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (145.85, 83.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.27, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.895 CPU sec, 7.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12972, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 107, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (131.00, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (183.90, 91.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.985 CPU sec, 8.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12559, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 94, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (114.81, 64.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (186.95, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.061 CPU sec, 8.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12711, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (134.94, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.61, 100.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.477 CPU sec, 8.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 12732, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (136.54, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (191.47, 99.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 262.191 CPU sec, 9.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12712, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 167, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (164.47, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (192.83, 100.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 265.405 CPU sec, 9.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12566, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 121, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (134.09, 87.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (189.60, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.771 CPU sec, 9.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12614, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 149, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (151.74, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (189.42, 97.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.700 CPU sec, 9.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12753, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 125, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (137.29, 76.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.48, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 287.820 CPU sec, 10.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12932, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (173.50, 99.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.14, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.401 CPU sec, 8.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13016, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 133, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (148.84, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.56, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.100 CPU sec, 9.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13219, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 85, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (92.62, 47.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (182.66, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.015 CPU sec, 8.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13066, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 141, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (158.74, 97.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (181.65, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.139 CPU sec, 8.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12648, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 152, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (155.77, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (180.30, 87.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.312 CPU sec, 8.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12651, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (123.84, 79.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (190.03, 96.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.005 CPU sec, 8.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12566, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (119.23, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (191.91, 99.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 271.367 CPU sec, 9.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12620, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 101, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (102.43, 52.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (189.02, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 279.306 CPU sec, 9.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12494, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 109, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (114.88, 56.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.18, 94.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 294.938 CPU sec, 10.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12500, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 108, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (110.00, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.68, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 290.096 CPU sec, 10.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12756, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 141, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (133.53, 60.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.25, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.919 CPU sec, 9.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12783, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (136.12, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (183.37, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.587 CPU sec, 9.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12858, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (124.13, 80.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.16, 91.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.471 CPU sec, 9.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 13312, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 96, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (103.67, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (179.87, 87.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.220 CPU sec, 7.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13080, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (137.61, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (180.19, 87.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.456 CPU sec, 7.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12928, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 109, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (118.00, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (178.21, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.549 CPU sec, 8.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12678, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 121, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (126.61, 73.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.01, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.903 CPU sec, 10.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12630, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 134, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (133.21, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (192.08, 98.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 265.665 CPU sec, 9.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12561, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (137.48, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (189.70, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.042 CPU sec, 9.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12755, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 127, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (123.70, 54.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.22, 95.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 280.545 CPU sec, 10.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12846, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 141, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (181.00, 113.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.11, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 286.555 CPU sec, 10.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12887, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 128, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (130.32, 63.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.24, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 273.504 CPU sec, 9.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12770, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 99, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (123.12, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (189.48, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.488 CPU sec, 9.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12976, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (111.73, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.76, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.375 CPU sec, 9.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13175, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (130.51, 63.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (181.87, 89.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.871 CPU sec, 7.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 13175, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 89, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (109.68, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (181.55, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.496 CPU sec, 7.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12922, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 75, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (77.55, 39.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (178.20, 85.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.213 CPU sec, 8.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12513, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (131.83, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.73, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.933 CPU sec, 8.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12407, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (116.52, 66.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (188.35, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.093 CPU sec, 9.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12507, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 126, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (124.08, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.53, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 273.247 CPU sec, 9.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12470, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 98, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (157.40, 112.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (189.44, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 278.465 CPU sec, 9.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12378, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (141.09, 76.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (190.14, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 301.390 CPU sec, 10.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 12534, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (121.58, 62.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.45, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 280.843 CPU sec, 9.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12399, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 100, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (110.41, 67.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (187.97, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 299.547 CPU sec, 10.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12751, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (129.06, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (185.64, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.888 CPU sec, 9.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13118, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (142.64, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.48, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.721 CPU sec, 8.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13083, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (121.48, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.98, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.338 CPU sec, 7.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13028, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 158, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (164.25, 107.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (177.94, 84.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.383 CPU sec, 8.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12884, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 114, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (139.58, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (182.90, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.951 CPU sec, 8.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 13222, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 120, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (110.94, 55.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.21, 93.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.893 CPU sec, 8.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13182, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (130.58, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (183.56, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.677 CPU sec, 8.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12878, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (116.66, 47.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (186.16, 94.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.348 CPU sec, 9.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 12825, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (151.77, 125.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.10, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.324 CPU sec, 9.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12949, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (141.56, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.30, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 289.913 CPU sec, 10.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13009, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 120, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (142.03, 88.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.35, 96.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.899 CPU sec, 9.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13000, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 128, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (145.14, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.89, 96.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.877 CPU sec, 9.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12987, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 141, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (174.74, 107.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.76, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 264.969 CPU sec, 9.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12761, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 130, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (170.57, 126.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.58, 96.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 264.493 CPU sec, 9.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12986, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 110, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (117.03, 57.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (190.21, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.942 CPU sec, 9.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13030, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (143.22, 90.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.40, 94.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.645 CPU sec, 8.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13226, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 116, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (122.88, 77.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.50, 94.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.966 CPU sec, 8.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13233, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 124, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (132.51, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.95, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.204 CPU sec, 7.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13206, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 99, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (86.22, 32.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.20, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.069 CPU sec, 9.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12886, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 96, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (101.68, 49.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.73, 96.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.406 CPU sec, 9.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12895, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 122, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (132.86, 65.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.32, 94.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 281.602 CPU sec, 9.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13120, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 170, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (175.07, 117.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (190.32, 98.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.059 CPU sec, 10.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12768, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 155, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (172.53, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.83, 93.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.416 CPU sec, 8.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12988, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 120, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (144.41, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.18, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.488 CPU sec, 9.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12747, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 121, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (117.20, 61.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (189.32, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.425 CPU sec, 8.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12798, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (117.05, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (189.20, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.314 CPU sec, 9.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 13286, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (134.33, 80.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (186.05, 93.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.292 CPU sec, 8.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13360, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (160.04, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (182.97, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.874 CPU sec, 8.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 13305, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 81, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (99.95, 58.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (181.96, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.576 CPU sec, 8.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13125, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (146.79, 105.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (188.52, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.546 CPU sec, 8.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 12848, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (147.60, 96.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.68, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.822 CPU sec, 9.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12864, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 163, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (155.46, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.81, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.492 CPU sec, 9.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12946, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (133.06, 91.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (187.14, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.813 CPU sec, 8.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12814, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (129.53, 76.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.37, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.688 CPU sec, 8.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12874, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (134.19, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.47, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.210 CPU sec, 8.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12799, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 100, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (107.82, 62.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.33, 94.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.984 CPU sec, 9.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12978, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (126.33, 86.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (190.88, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.650 CPU sec, 9.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13024, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 118, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (119.92, 50.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.78, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.533 CPU sec, 8.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13186, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (130.82, 78.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (183.24, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.194 CPU sec, 9.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13292, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 145, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (136.63, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.54, 90.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.480 CPU sec, 8.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12429, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (135.14, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (188.45, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 313.142 CPU sec, 10.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12538, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (111.77, 61.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (185.98, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 308.469 CPU sec, 11.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12470, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 114, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (131.39, 70.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.74, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 284.206 CPU sec, 9.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12706, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 78, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (85.59, 60.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.67, 90.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.206 CPU sec, 10.071 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12613, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.46, 80.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (178.45, 85.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.134 CPU sec, 8.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12893, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (114.41, 70.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.37, 90.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 271.142 CPU sec, 9.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12915, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 126, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (162.31, 119.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (184.87, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 276.530 CPU sec, 9.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12662, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (206.58, 158.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (184.07, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 276.492 CPU sec, 9.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13319, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 95, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (126.74, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (186.18, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.547 CPU sec, 9.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13173, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (137.52, 66.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (182.80, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.730 CPU sec, 8.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 13009, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 100, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (137.49, 105.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (180.47, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.289 CPU sec, 8.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12582, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 115, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (142.43, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.93, 94.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 294.651 CPU sec, 10.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12762, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (153.59, 98.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.14, 94.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 308.094 CPU sec, 10.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12896, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 102, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (100.91, 40.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (187.09, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 282.662 CPU sec, 10.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12847, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 123, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (135.81, 81.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (186.02, 92.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.298 CPU sec, 8.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12909, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 95, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (101.97, 63.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 161, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.12, 87.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.330 CPU sec, 9.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12763, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 135, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (165.08, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (184.60, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 262.017 CPU sec, 9.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12868, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (126.14, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (185.59, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 273.726 CPU sec, 9.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13009, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (124.78, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.38, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 263.780 CPU sec, 9.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 13350, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (147.93, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.80, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.564 CPU sec, 8.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 13194, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 139, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (146.18, 103.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (181.31, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.851 CPU sec, 9.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13158, 8, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 109, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (116.09, 59.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (182.77, 90.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.326 CPU sec, 7.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12791, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 125, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (122.41, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.57, 94.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.416 CPU sec, 9.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12659, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 102, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (104.14, 54.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.98, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.427 CPU sec, 9.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12789, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 100, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (147.13, 131.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (185.03, 91.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.399 CPU sec, 9.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12887, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 125, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (147.86, 91.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (182.41, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 261.563 CPU sec, 9.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12732, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (151.27, 94.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (182.10, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.349 CPU sec, 9.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12964, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (122.74, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.06, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.878 CPU sec, 8.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12877, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 144, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (159.60, 92.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.86, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.708 CPU sec, 8.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12929, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 127, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (139.61, 85.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.94, 92.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.190 CPU sec, 9.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12987, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 121, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (161.34, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (179.58, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.016 CPU sec, 8.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13145, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (126.53, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (177.21, 84.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.787 CPU sec, 7.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12702, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (138.15, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (182.03, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.201 CPU sec, 9.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12728, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (119.06, 61.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (188.80, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.257 CPU sec, 9.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12794, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 136, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (134.00, 63.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.92, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.077 CPU sec, 9.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13114, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (135.74, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 167, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.17, 96.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.859 CPU sec, 8.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13041, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (128.08, 83.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (186.23, 94.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.456 CPU sec, 8.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12733, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 138, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (149.94, 86.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (188.02, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.437 CPU sec, 9.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12710, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (129.62, 93.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (192.91, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.843 CPU sec, 8.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12756, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 99, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (135.72, 102.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.24, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.402 CPU sec, 9.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12812, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 115, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (130.15, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (186.10, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.337 CPU sec, 9.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13151, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (135.00, 79.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (183.62, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.901 CPU sec, 8.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13053, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 108, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (107.27, 47.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (178.10, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.595 CPU sec, 9.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12589, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (122.81, 63.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (185.08, 93.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.953 CPU sec, 9.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12834, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 115, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (117.12, 48.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (191.01, 98.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.478 CPU sec, 9.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12742, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (130.92, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.26, 94.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.199 CPU sec, 8.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12825, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (150.94, 118.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.57, 94.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.692 CPU sec, 7.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 12877, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (123.47, 87.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.07, 96.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.876 CPU sec, 7.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12695, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (130.28, 80.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (190.59, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.181 CPU sec, 7.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12652, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (133.27, 83.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (190.32, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.462 CPU sec, 7.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12537, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (145.43, 124.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 169, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.39, 98.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.783 CPU sec, 8.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12949, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 113, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (129.49, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (188.64, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.285 CPU sec, 7.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13151, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 136, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (154.53, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.49, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.126 CPU sec, 8.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13023, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 111, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (126.36, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (180.93, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.099 CPU sec, 7.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12702, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 157, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (158.61, 82.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.21, 90.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.168 CPU sec, 8.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12928, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 93, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (129.71, 82.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (190.67, 98.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.484 CPU sec, 9.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12983, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 88, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (125.08, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.57, 97.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.993 CPU sec, 8.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12976, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 117, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (119.31, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.05, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.036 CPU sec, 8.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12998, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 151, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (164.04, 91.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.74, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.117 CPU sec, 9.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12791, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 104, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (115.77, 64.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (190.93, 98.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.699 CPU sec, 7.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13027, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 98, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (113.72, 67.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (190.73, 99.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.018 CPU sec, 9.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12670, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (152.12, 105.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.40, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.516 CPU sec, 8.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 13004, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 108, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (115.17, 67.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (180.80, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.795 CPU sec, 8.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13273, 1, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (127.09, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (180.36, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.602 CPU sec, 8.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12768, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (126.62, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (176.37, 83.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.188 CPU sec, 7.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12551, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 102, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (135.92, 96.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (182.31, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.150 CPU sec, 8.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12440, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (116.61, 62.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.60, 91.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 275.623 CPU sec, 9.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12632, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 102, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (108.95, 58.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.17, 95.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.270 CPU sec, 8.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12768, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (150.42, 110.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (185.18, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.130 CPU sec, 9.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12666, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 152, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (163.56, 113.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.83, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.576 CPU sec, 8.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12598, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 91, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (116.48, 61.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.59, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.038 CPU sec, 8.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12640, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 101, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (120.57, 69.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (188.31, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.813 CPU sec, 7.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12510, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 109, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (121.11, 56.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.59, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.211 CPU sec, 8.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13481, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 86, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (119.19, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (183.60, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.338 CPU sec, 8.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13231, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 112, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (121.73, 74.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (179.03, 86.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.349 CPU sec, 6.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13095, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (119.75, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (174.76, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.390 CPU sec, 6.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12604, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 97, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (140.96, 94.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (184.67, 92.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.234 CPU sec, 7.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12653, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 130, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (140.70, 77.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.85, 95.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.727 CPU sec, 7.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12611, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 154, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (147.14, 92.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.81, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.102 CPU sec, 8.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12948, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 154, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (139.52, 65.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (182.28, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.754 CPU sec, 8.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12683, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (126.87, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (185.83, 94.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.246 CPU sec, 7.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12625, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (167.29, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.72, 93.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.129 CPU sec, 7.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12653, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (135.41, 85.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.58, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.046 CPU sec, 7.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12728, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 94, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (108.23, 63.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 167, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (189.13, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.721 CPU sec, 7.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13195, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 95, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (114.36, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.32, 90.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.962 CPU sec, 7.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13098, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 98, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (104.12, 55.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (182.87, 89.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.647 CPU sec, 6.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12834, 6, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (118.00, 70.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 159, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (177.43, 84.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.506 CPU sec, 6.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12753, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 105, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (117.43, 77.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (184.98, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.461 CPU sec, 7.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12709, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 117, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (133.28, 68.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (187.10, 95.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.786 CPU sec, 8.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12862, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 98, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (93.21, 43.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (184.08, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.570 CPU sec, 8.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12969, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 105, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (126.27, 72.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (186.17, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.927 CPU sec, 7.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12961, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 92, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (98.91, 38.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.44, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.930 CPU sec, 7.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12759, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (90.70, 37.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.73, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.390 CPU sec, 7.096 real sec [M::process] read 51246 sequences (7738146 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12729, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 184, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (158.81, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (185.47, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.501 CPU sec, 7.704 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 9937, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (135.36, 89.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.01, 96.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 51246 reads in 167.174 CPU sec, 6.148 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21160\tSM:UG10S2P111\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21160_HLKNYBGXX_UG10S2P111_R1.fastq.gz 5347_7332_21160_HLKNYBGXX_UG10S2P111_R2.fastq.gz [main] Real time: 2396.610 sec; CPU: 66642.875 sec Finished alignement of UG10S2P111 at Wed Apr 3 05:14:00 2019 Sorting BAM file started at Wed Apr 3 05:14:00 2019 samtools sort -@ 30 -m 4G UG10S2P111_unsorted.bam -o UG10S2P111.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 05:14:00 2019 rm UG10S2P111_unsorted.bam rm 5347_7332_21160_HLKNYBGXX_UG10S2P111_R1.fastq.gz rm 5347_7332_21160_HLKNYBGXX_UG10S2P111_R2.fastq.gz samtools index UG10S2P111.bam Finished BAM file generation of the sample - UG10S2P111 Processing fastq files for the sample - UG10S2P115 Reading 5347_7332_21161_HLKNYBGXX_UG10S2P115_R1.fastq.gz 5347_7332_21161_HLKNYBGXX_UG10S2P115_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21161\tSM:UG10S2P115\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21161_HLKNYBGXX_UG10S2P115_R1.fastq.gz 5347_7332_21161_HLKNYBGXX_UG10S2P115_R2.fastq.gz | samtools view -Sb1 - > UG10S2P115_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14311, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 154, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (159.89, 83.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.04, 95.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.890 CPU sec, 7.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14568, 8, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 101, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (117.47, 70.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.68, 94.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.689 CPU sec, 6.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14459, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 113, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (130.89, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.58, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.740 CPU sec, 7.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14308, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 172, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (179.51, 112.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (204.08, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.379 CPU sec, 7.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 13999, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (147.40, 94.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.69, 95.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.457 CPU sec, 7.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13901, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 102, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (121.00, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 184, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (203.98, 95.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.521 CPU sec, 8.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13936, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 160, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (158.23, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.27, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.034 CPU sec, 7.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14155, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (147.54, 109.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (200.85, 92.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.129 CPU sec, 6.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14370, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 112, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (120.56, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.41, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.018 CPU sec, 6.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14856, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (128.15, 81.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.92, 92.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.670 CPU sec, 5.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14908, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (133.41, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.46, 92.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.851 CPU sec, 5.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 14995, 3, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 125, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (137.00, 81.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.81, 90.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.772 CPU sec, 5.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14556, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (114.18, 54.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (202.18, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.909 CPU sec, 7.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14384, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (121.12, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.77, 96.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.859 CPU sec, 7.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 14368, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (140.19, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.81, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.061 CPU sec, 7.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 14513, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 152, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (148.80, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (203.56, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.096 CPU sec, 6.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14419, 8, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 145, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (141.28, 79.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (201.23, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.394 CPU sec, 7.799 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14153, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 111, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (161.11, 109.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (204.06, 96.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.749 CPU sec, 7.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14185, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (128.96, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (201.12, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.422 CPU sec, 7.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14400, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 132, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (139.31, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (201.86, 94.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.727 CPU sec, 6.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14690, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 135, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (152.50, 100.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (200.18, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.940 CPU sec, 6.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14982, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 126, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (136.41, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.84, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.335 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14965, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (145.50, 101.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.69, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.367 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14911, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (138.26, 95.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.82, 91.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.028 CPU sec, 6.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14804, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 107, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (120.12, 58.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.82, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.684 CPU sec, 6.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14306, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 96, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (118.23, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.16, 92.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.435 CPU sec, 7.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14448, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (134.15, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.58, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.531 CPU sec, 7.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14490, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 131, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (161.82, 104.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (202.39, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.950 CPU sec, 6.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14127, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 100, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (99.44, 49.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (203.12, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.499 CPU sec, 6.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 13933, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 109, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (120.40, 75.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.66, 95.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.913 CPU sec, 7.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14096, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 121, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (149.56, 95.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.13, 92.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.239 CPU sec, 8.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14236, 8, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (160.52, 113.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.98, 92.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.954 CPU sec, 7.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14298, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 101, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (123.50, 85.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (199.18, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.090 CPU sec, 7.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 15002, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 108, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (124.38, 66.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.01, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.675 CPU sec, 6.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14908, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 142, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (156.30, 112.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.31, 91.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.772 CPU sec, 5.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 15141, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 114, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (153.52, 100.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.57, 89.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.523 CPU sec, 5.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14659, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 92, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (133.97, 91.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.87, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.547 CPU sec, 6.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 14436, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 140, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (154.66, 99.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.98, 92.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.636 CPU sec, 7.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14341, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 102, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (103.19, 54.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.15, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.869 CPU sec, 6.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14389, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 133, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (151.18, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (202.05, 94.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.884 CPU sec, 7.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14179, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 115, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (138.30, 81.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.97, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.053 CPU sec, 7.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13913, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (121.35, 61.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.40, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.430 CPU sec, 7.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 13703, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 116, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (139.12, 78.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.00, 93.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.095 CPU sec, 9.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 13813, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 92, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (118.11, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.84, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.887 CPU sec, 7.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 13845, 4, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 109, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (101.74, 45.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.71, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.923 CPU sec, 7.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14465, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 98, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (122.94, 66.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.87, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.818 CPU sec, 6.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14737, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 119, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (140.33, 87.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.54, 90.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.127 CPU sec, 5.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14665, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 121, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (128.34, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (197.56, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.166 CPU sec, 6.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14760, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 104, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (111.15, 56.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.98, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.803 CPU sec, 5.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14465, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (123.70, 66.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.84, 89.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.330 CPU sec, 6.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 14364, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (137.31, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.05, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.298 CPU sec, 7.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14434, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 149, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (164.20, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.17, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.971 CPU sec, 7.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14365, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (173.45, 92.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.89, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.236 CPU sec, 6.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14018, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (117.90, 61.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.18, 91.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.358 CPU sec, 7.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 13831, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 103, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (126.35, 87.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (200.32, 94.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.366 CPU sec, 8.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 13873, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (139.26, 73.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (197.02, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.091 CPU sec, 8.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14205, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (165.71, 109.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.29, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.408 CPU sec, 7.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14448, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (126.03, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.76, 91.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.245 CPU sec, 7.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14910, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (163.98, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (196.81, 90.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.860 CPU sec, 6.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14891, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 128, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (143.27, 84.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.92, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.333 CPU sec, 5.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 14975, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 131, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (128.96, 70.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (197.22, 90.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.433 CPU sec, 5.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14426, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 137, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (148.07, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.92, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.064 CPU sec, 6.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 14241, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 108, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (147.88, 102.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.46, 91.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.306 CPU sec, 6.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 14118, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 98, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (115.10, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.01, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.975 CPU sec, 7.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14171, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (112.00, 63.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.18, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.289 CPU sec, 7.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13928, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 98, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (109.76, 75.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.22, 91.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.733 CPU sec, 7.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13620, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 137, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (142.05, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.57, 94.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.271 CPU sec, 8.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13801, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 96, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (105.39, 63.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (198.80, 91.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.384 CPU sec, 7.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 13879, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (107.41, 58.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.80, 94.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.608 CPU sec, 7.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14055, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (136.65, 82.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.73, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.248 CPU sec, 7.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14618, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (126.66, 80.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.81, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.860 CPU sec, 6.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14859, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (157.76, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (196.26, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.336 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 15125, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (149.11, 91.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.44, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.272 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 14385, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (132.57, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.23, 95.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.080 CPU sec, 6.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14108, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 97, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (108.84, 58.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (200.18, 92.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.080 CPU sec, 6.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14226, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 87, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (119.95, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (203.34, 95.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.554 CPU sec, 7.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14201, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (171.62, 121.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.43, 94.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.596 CPU sec, 6.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 13990, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 102, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (118.86, 76.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (201.36, 93.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.971 CPU sec, 7.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 13966, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (135.65, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.85, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.953 CPU sec, 7.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14167, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 167, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (172.71, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (204.48, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.317 CPU sec, 7.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14482, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 118, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (135.40, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.34, 93.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.111 CPU sec, 7.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14335, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (114.16, 65.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.48, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.196 CPU sec, 7.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14825, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (122.74, 79.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (197.17, 89.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.956 CPU sec, 6.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14953, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 111, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (133.00, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.70, 91.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.102 CPU sec, 5.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14573, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (110.03, 64.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (194.02, 85.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.545 CPU sec, 6.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14397, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (127.24, 68.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.78, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.640 CPU sec, 7.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14140, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (115.94, 67.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (205.67, 97.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.258 CPU sec, 6.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14191, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 132, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (137.32, 75.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 184, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (204.43, 96.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.840 CPU sec, 7.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14044, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (112.51, 70.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (203.55, 96.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.112 CPU sec, 7.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 13980, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 148, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (137.31, 81.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (204.29, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.816 CPU sec, 7.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14069, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 114, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (150.09, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (203.28, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.202 CPU sec, 8.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14024, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 124, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (128.35, 86.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.62, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.864 CPU sec, 7.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14207, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 118, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (155.84, 96.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.70, 94.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.025 CPU sec, 7.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14232, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (148.68, 102.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.31, 94.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.847 CPU sec, 7.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14621, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (136.51, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.34, 93.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.294 CPU sec, 6.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 14859, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 142, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (152.58, 80.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (194.96, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.927 CPU sec, 6.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 14590, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (126.95, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (194.39, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.763 CPU sec, 5.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14358, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (128.15, 81.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.92, 91.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.848 CPU sec, 6.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 13950, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 131, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (155.67, 95.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (206.68, 97.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.509 CPU sec, 7.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13885, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (151.12, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.75, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.871 CPU sec, 6.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14047, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 127, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (148.11, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.00, 94.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.879 CPU sec, 7.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13952, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 94, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (128.65, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 182, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (204.20, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.155 CPU sec, 7.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14004, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (128.14, 105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.51, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.777 CPU sec, 7.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14000, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (128.96, 81.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (201.30, 94.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.325 CPU sec, 7.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14215, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 114, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (131.42, 70.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.29, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.251 CPU sec, 7.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14390, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (136.00, 76.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.41, 93.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.021 CPU sec, 6.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 14632, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (128.19, 60.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.45, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.991 CPU sec, 6.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14813, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 111, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (100.68, 39.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (195.94, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.692 CPU sec, 5.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14835, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (120.27, 53.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (193.70, 86.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.768 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 14524, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (158.67, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (195.15, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.797 CPU sec, 6.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13959, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 119, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (137.85, 89.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (201.04, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.231 CPU sec, 7.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13932, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 126, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (126.65, 63.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (200.70, 93.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.711 CPU sec, 7.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13886, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 110, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (120.20, 70.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.12, 93.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.844 CPU sec, 6.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13699, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (132.76, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (199.10, 90.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.270 CPU sec, 7.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13927, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 143, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (156.09, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.33, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.621 CPU sec, 8.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13700, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (132.72, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (200.01, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.037 CPU sec, 9.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14030, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (128.89, 71.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (200.15, 93.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.704 CPU sec, 7.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14173, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (117.02, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (199.29, 90.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.497 CPU sec, 7.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 14319, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (136.88, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.47, 91.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.171 CPU sec, 7.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14661, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (134.69, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.04, 88.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.110 CPU sec, 5.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14850, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 102, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (111.53, 75.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (194.94, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.970 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14701, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (149.03, 106.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (193.05, 86.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.154 CPU sec, 5.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14240, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 118, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (153.11, 121.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (197.79, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.944 CPU sec, 6.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13879, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (119.03, 59.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.29, 95.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.028 CPU sec, 7.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13841, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 123, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (146.61, 107.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.44, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.294 CPU sec, 7.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 13876, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (106.65, 54.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (203.79, 95.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.610 CPU sec, 7.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 13891, 9, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 122, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (148.95, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.88, 93.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.085 CPU sec, 7.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 13947, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 139, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (152.95, 88.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.22, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.403 CPU sec, 8.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13868, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 102, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (156.46, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.53, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.313 CPU sec, 7.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14040, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 148, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (157.36, 102.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.10, 93.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.579 CPU sec, 7.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14361, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (134.82, 90.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (198.96, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.011 CPU sec, 7.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14868, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (134.59, 86.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.27, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.755 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 14963, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (131.04, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.05, 86.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.162 CPU sec, 6.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14676, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 125, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (133.65, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (190.70, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.942 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14241, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 129, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (132.52, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.06, 87.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.200 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13673, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 129, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (139.00, 85.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (205.48, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.904 CPU sec, 6.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13856, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 123, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (131.38, 75.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.94, 95.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.023 CPU sec, 6.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 13718, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (137.51, 79.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.35, 95.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.146 CPU sec, 7.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13633, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 156, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (178.24, 97.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (203.97, 96.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.758 CPU sec, 8.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13591, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 103, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (114.30, 71.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (202.23, 94.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.525 CPU sec, 8.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13613, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 94, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (130.10, 100.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (202.56, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.538 CPU sec, 8.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14094, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (158.39, 87.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.86, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.058 CPU sec, 6.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 13944, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 95, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (109.02, 69.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.71, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.524 CPU sec, 6.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 14500, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 115, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (133.32, 72.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (195.54, 87.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.442 CPU sec, 7.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14831, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 107, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (125.12, 78.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.04, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.959 CPU sec, 6.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 14562, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (147.98, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.60, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.896 CPU sec, 6.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 14518, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 130, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (139.94, 82.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (190.37, 83.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.087 CPU sec, 6.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14707, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 126, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (139.42, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (198.22, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.390 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 15029, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 147, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (161.85, 105.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (195.36, 88.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.671 CPU sec, 5.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 14875, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 112, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (125.16, 66.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.30, 90.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.176 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14508, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (133.62, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (198.25, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.112 CPU sec, 6.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14155, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 152, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (169.22, 94.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.85, 93.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.321 CPU sec, 7.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14250, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (158.98, 104.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (200.73, 92.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.677 CPU sec, 8.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14373, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (114.39, 48.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (201.26, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.401 CPU sec, 7.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14529, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 124, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (130.92, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.32, 92.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.502 CPU sec, 8.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14349, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 92, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (121.26, 66.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.75, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.904 CPU sec, 6.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14235, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 122, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (129.98, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (200.28, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.103 CPU sec, 7.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 14221, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 121, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (147.80, 86.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.22, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.328 CPU sec, 7.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14213, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 114, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (159.05, 106.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.62, 93.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.184 CPU sec, 7.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14949, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 90, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (120.54, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (199.13, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.872 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14686, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 109, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (127.44, 67.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.38, 89.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.365 CPU sec, 5.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14876, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (135.65, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.22, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.315 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14318, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 131, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (135.33, 83.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (199.18, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.744 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14373, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 133, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (145.74, 103.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.65, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.915 CPU sec, 7.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14228, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 119, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (154.62, 129.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (202.98, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.890 CPU sec, 7.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14479, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 110, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (139.90, 90.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.61, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.779 CPU sec, 8.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14666, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 128, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (145.42, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.53, 93.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.962 CPU sec, 6.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14325, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 124, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (138.88, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.83, 92.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.974 CPU sec, 6.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14249, 1, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 144, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (153.28, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.80, 93.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.315 CPU sec, 7.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 14559, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (140.14, 78.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.87, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.886 CPU sec, 8.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14337, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 88, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (104.24, 68.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.13, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.236 CPU sec, 7.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 14939, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (124.61, 70.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.84, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.072 CPU sec, 5.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 15234, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 134, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (156.35, 84.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.34, 88.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.819 CPU sec, 5.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 14816, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 121, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (137.30, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.28, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.312 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14336, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 91, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (122.78, 78.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.65, 93.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.197 CPU sec, 6.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14314, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (183.11, 97.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (201.04, 93.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.734 CPU sec, 7.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14589, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (146.07, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.75, 93.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.628 CPU sec, 6.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14637, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 133, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (128.53, 54.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.15, 90.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.123 CPU sec, 6.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14446, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 88, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (106.05, 65.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.25, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.418 CPU sec, 6.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14289, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 108, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (117.53, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.06, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.432 CPU sec, 7.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14314, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 133, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (140.67, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.91, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.535 CPU sec, 7.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 14312, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (140.52, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.54, 94.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.660 CPU sec, 7.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14356, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 97, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (119.00, 86.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.16, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.842 CPU sec, 6.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14833, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (161.65, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (194.74, 86.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.010 CPU sec, 5.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 14902, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (125.13, 65.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (194.38, 86.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.567 CPU sec, 6.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14678, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (133.10, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.48, 89.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.222 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 13948, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (128.35, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.99, 92.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.017 CPU sec, 8.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13730, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (170.13, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (198.59, 91.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.064 CPU sec, 8.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 13907, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 128, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (151.33, 108.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (199.69, 92.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.701 CPU sec, 8.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14074, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (133.53, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.95, 90.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.919 CPU sec, 6.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14455, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (128.12, 80.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (195.61, 88.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.785 CPU sec, 6.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14260, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 115, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (131.56, 80.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.85, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.562 CPU sec, 8.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14079, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (135.61, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.59, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.056 CPU sec, 7.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14239, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 145, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (131.37, 76.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.11, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.825 CPU sec, 7.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14614, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (126.03, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.43, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.066 CPU sec, 6.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14854, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 104, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (149.32, 112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.73, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.133 CPU sec, 5.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14819, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 99, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (116.77, 63.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.50, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.640 CPU sec, 6.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14779, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (146.42, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (195.50, 88.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.825 CPU sec, 6.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14036, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (140.43, 87.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (198.72, 92.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.612 CPU sec, 8.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13956, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (125.21, 54.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.96, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.839 CPU sec, 8.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14280, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 100, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (120.23, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.41, 92.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.517 CPU sec, 7.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14492, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (128.85, 73.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (197.01, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.619 CPU sec, 6.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14436, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 112, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (122.19, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (195.00, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.154 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14129, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 99, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (108.66, 57.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (197.33, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.854 CPU sec, 7.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14313, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (149.88, 85.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (199.05, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.993 CPU sec, 7.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14343, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 109, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (125.95, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.98, 87.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.306 CPU sec, 7.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14618, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 107, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (111.95, 56.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (195.70, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.469 CPU sec, 6.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14741, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (134.78, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (193.75, 86.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.185 CPU sec, 6.071 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14915, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (136.42, 89.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (195.36, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.346 CPU sec, 5.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14443, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 98, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (116.16, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.76, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.342 CPU sec, 6.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14078, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (118.59, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.42, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.108 CPU sec, 7.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14152, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 102, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (129.88, 76.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.42, 90.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.923 CPU sec, 7.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14086, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 123, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (139.49, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.92, 89.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.968 CPU sec, 7.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14329, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 128, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (151.23, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.44, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.976 CPU sec, 7.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14405, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (144.38, 78.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (197.69, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.259 CPU sec, 6.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14260, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 88, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (99.34, 58.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (195.42, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.180 CPU sec, 7.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 14440, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 119, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (142.02, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.83, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.524 CPU sec, 6.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14420, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 109, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (130.17, 73.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (199.64, 92.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.194 CPU sec, 6.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 14962, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 117, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (131.08, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.95, 88.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.529 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14948, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 114, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (129.98, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.10, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.246 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 14565, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 127, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (136.35, 92.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (194.57, 87.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.621 CPU sec, 5.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14208, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 125, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (138.55, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.82, 90.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.267 CPU sec, 7.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14163, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (133.57, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (202.03, 94.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.676 CPU sec, 6.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14316, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (146.00, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.64, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.354 CPU sec, 6.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14349, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (133.47, 94.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.13, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.386 CPU sec, 7.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 14412, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 110, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (118.46, 59.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.13, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.288 CPU sec, 6.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14258, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (137.23, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.62, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.960 CPU sec, 6.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14152, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (145.43, 70.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (204.53, 96.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.804 CPU sec, 6.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14104, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (125.88, 78.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.87, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.769 CPU sec, 7.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14199, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (136.45, 77.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (200.75, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.085 CPU sec, 7.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14723, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 116, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (121.65, 68.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.38, 88.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.501 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 14839, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 119, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (118.64, 55.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (196.61, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.024 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 14719, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 124, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (140.20, 92.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (194.24, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.984 CPU sec, 6.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14217, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 147, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (151.07, 82.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.98, 93.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.846 CPU sec, 7.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 13947, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 87, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (113.23, 75.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (201.31, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.702 CPU sec, 9.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14237, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (138.36, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.30, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.365 CPU sec, 7.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14329, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 112, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (164.95, 106.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.84, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.597 CPU sec, 7.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14163, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (136.57, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.96, 93.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.294 CPU sec, 7.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 13868, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (118.26, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.70, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.318 CPU sec, 7.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14065, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 120, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (128.52, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.25, 94.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.391 CPU sec, 7.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 13919, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 92, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (123.96, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.66, 95.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.314 CPU sec, 7.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 14508, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (138.49, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (199.88, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.474 CPU sec, 6.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14492, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (136.36, 89.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.82, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.039 CPU sec, 5.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14717, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 87, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (97.39, 49.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.20, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.402 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14650, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 115, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (150.67, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.01, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.684 CPU sec, 5.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14263, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 107, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (121.44, 79.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.82, 91.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.728 CPU sec, 6.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14456, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (128.89, 76.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (201.68, 93.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.767 CPU sec, 6.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14327, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 106, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (133.30, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (200.77, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.825 CPU sec, 6.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14485, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 102, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (131.23, 92.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.32, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.163 CPU sec, 7.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14306, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 82, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (95.75, 56.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.41, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.947 CPU sec, 7.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14299, 3, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (130.12, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.60, 94.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.317 CPU sec, 7.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14227, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 130, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (140.34, 82.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.95, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.686 CPU sec, 7.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14242, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 105, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (143.49, 104.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.43, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.713 CPU sec, 7.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14420, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (133.28, 77.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.98, 90.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.400 CPU sec, 6.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14644, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 123, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (121.66, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.01, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.676 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 14489, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 114, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (143.92, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (191.08, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.546 CPU sec, 5.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14386, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (147.02, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (191.75, 83.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.873 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13868, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 108, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (124.33, 78.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.86, 92.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.245 CPU sec, 8.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 13931, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 103, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (116.20, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (198.08, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.740 CPU sec, 9.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 13919, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 102, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (113.92, 57.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.06, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.781 CPU sec, 7.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14023, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 101, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (108.05, 58.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (196.66, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.151 CPU sec, 7.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13987, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (131.63, 65.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (198.89, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.255 CPU sec, 6.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 14017, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 151, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (167.59, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (198.41, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.054 CPU sec, 6.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13852, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (135.75, 77.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.39, 92.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.758 CPU sec, 7.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13906, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 103, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (97.67, 49.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.60, 91.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.239 CPU sec, 9.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14390, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 103, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (113.83, 63.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (195.75, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.334 CPU sec, 6.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 14853, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (130.67, 77.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (189.71, 83.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.046 CPU sec, 5.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 14670, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (146.23, 84.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.69, 84.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.015 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 14406, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.07, 62.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (190.74, 83.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.157 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 13926, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 94, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (115.80, 78.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (198.33, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.696 CPU sec, 7.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13924, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (145.15, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.40, 93.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.665 CPU sec, 8.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14165, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (132.54, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (197.40, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.819 CPU sec, 6.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14444, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (140.45, 86.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (197.31, 90.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.139 CPU sec, 7.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13898, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 124, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (139.97, 91.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (197.59, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.654 CPU sec, 6.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14129, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 97, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (106.00, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (199.98, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.761 CPU sec, 6.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 13741, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (148.39, 91.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.60, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.265 CPU sec, 7.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 13991, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 114, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (135.64, 86.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.93, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.014 CPU sec, 8.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14717, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 114, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (136.96, 84.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.35, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.554 CPU sec, 6.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14647, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (118.38, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (195.40, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.035 CPU sec, 6.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14620, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (136.89, 85.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (191.29, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.421 CPU sec, 5.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 14348, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 121, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (128.41, 53.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (191.83, 85.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.064 CPU sec, 5.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14032, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (138.28, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (201.10, 93.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.810 CPU sec, 7.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14038, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (134.40, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.57, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.800 CPU sec, 7.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 13895, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 121, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (119.63, 53.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.96, 91.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.914 CPU sec, 7.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14260, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 120, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (132.05, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.05, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.929 CPU sec, 7.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14028, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (131.14, 75.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.78, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.411 CPU sec, 7.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 14059, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (135.25, 71.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (201.05, 94.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.856 CPU sec, 7.329 real sec [M::process] read 65996 sequences (9965396 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14069, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (134.38, 63.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (198.68, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.620 CPU sec, 7.627 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 13975, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 122, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (124.51, 65.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.75, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 65996 reads in 200.798 CPU sec, 7.332 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21161\tSM:UG10S2P115\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21161_HLKNYBGXX_UG10S2P115_R1.fastq.gz 5347_7332_21161_HLKNYBGXX_UG10S2P115_R2.fastq.gz [main] Real time: 2017.834 sec; CPU: 55219.163 sec Finished alignement of UG10S2P115 at Wed Apr 3 05:55:12 2019 Sorting BAM file started at Wed Apr 3 05:55:12 2019 samtools sort -@ 30 -m 4G UG10S2P115_unsorted.bam -o UG10S2P115.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 05:55:12 2019 rm UG10S2P115_unsorted.bam rm 5347_7332_21161_HLKNYBGXX_UG10S2P115_R1.fastq.gz rm 5347_7332_21161_HLKNYBGXX_UG10S2P115_R2.fastq.gz samtools index UG10S2P115.bam Finished BAM file generation of the sample - UG10S2P115 Processing fastq files for the sample - UG10S1P021 Reading 5347_7332_21162_HLKNYBGXX_UG10S1P021_R1.fastq.gz 5347_7332_21162_HLKNYBGXX_UG10S1P021_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21162\tSM:UG10S1P021\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21162_HLKNYBGXX_UG10S1P021_R1.fastq.gz 5347_7332_21162_HLKNYBGXX_UG10S1P021_R2.fastq.gz | samtools view -Sb1 - > UG10S1P021_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11854, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 264, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (197.08, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.63, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.445 CPU sec, 7.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11812, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 146, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (135.36, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.57, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.971 CPU sec, 5.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12008, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 153, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.67, 76.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.57, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.015 CPU sec, 6.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11982, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 123, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (156.86, 104.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.09, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.485 CPU sec, 4.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11982, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.62, 93.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.893 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12103, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 133, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (139.57, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.39, 90.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.902 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12154, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (135.90, 64.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (197.23, 92.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.007 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12033, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 150, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (151.65, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.78, 92.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.702 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11803, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.14, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.452 CPU sec, 4.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11849, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (108.70, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.94, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.103 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11992, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (129.12, 86.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.55, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.923 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11958, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (113.33, 58.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.55, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.349 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11956, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 86, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (107.54, 59.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.01, 88.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.093 CPU sec, 5.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12072, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 107, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 205) [M::mem_pestat] mean and std.dev: (103.00, 25.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.72, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.068 CPU sec, 6.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12012, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (173.29, 112.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.82, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.698 CPU sec, 6.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11969, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 103, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (116.21, 56.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.96, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.363 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11822, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 126, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (145.69, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.30, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.506 CPU sec, 5.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11947, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 145, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (136.00, 42.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.26, 85.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.898 CPU sec, 5.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11908, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (139.89, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.46, 84.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.880 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11911, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 104, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (157.70, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.56, 87.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.183 CPU sec, 4.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11639, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 135, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (128.50, 67.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.15, 91.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.391 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11505, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 131, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (106.57, 48.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (193.76, 91.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.437 CPU sec, 6.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11750, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 87, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (134.80, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.72, 89.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.306 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11893, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 110, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (121.77, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (194.30, 90.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.864 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11893, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (178.79, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.31, 91.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.579 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12050, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 115, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (104.30, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.52, 88.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.635 CPU sec, 4.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11982, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (149.50, 89.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.95, 91.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.029 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12066, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (160.75, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (195.82, 92.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.819 CPU sec, 5.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12115, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 124, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (154.81, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.40, 91.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.836 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11922, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 111, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (139.88, 93.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.43, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.644 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12048, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 131, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (139.27, 68.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.36, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.581 CPU sec, 4.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11793, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (144.67, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.66, 91.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.273 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11987, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 98, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (120.73, 65.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.40, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.364 CPU sec, 5.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12029, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 128, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (153.73, 76.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.66, 90.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.748 CPU sec, 5.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11889, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (141.00, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.23, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.316 CPU sec, 4.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11965, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (146.50, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.86, 87.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.037 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11922, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (117.00, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.93, 86.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.578 CPU sec, 5.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11916, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 134, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (142.75, 107.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.31, 86.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.957 CPU sec, 4.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12016, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 98, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (109.19, 56.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.53, 86.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.367 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11861, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 143, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (118.92, 46.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.04, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.567 CPU sec, 5.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11956, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 128, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (162.64, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.73, 86.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.786 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11728, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (108.47, 48.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.28, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.364 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11671, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 108, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (124.56, 71.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.10, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.362 CPU sec, 6.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11729, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 98, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (106.07, 53.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.38, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.458 CPU sec, 5.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11888, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 148, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (126.92, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.29, 87.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.442 CPU sec, 4.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12001, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 71, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (113.00, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.50, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.749 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11877, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (132.38, 70.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.34, 88.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.618 CPU sec, 5.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12143, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 160, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (135.07, 68.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.35, 91.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.614 CPU sec, 5.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11980, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 125, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (157.84, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (192.66, 89.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.189 CPU sec, 5.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11836, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (150.70, 83.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.70, 91.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.728 CPU sec, 5.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12118, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 104, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (161.31, 107.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.85, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.098 CPU sec, 6.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12080, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 99, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (116.47, 60.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.05, 89.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.198 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11978, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 97, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (112.95, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.87, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.455 CPU sec, 4.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11934, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 112, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (117.53, 62.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.63, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.783 CPU sec, 5.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11929, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 129, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (113.28, 56.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.49, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.389 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11917, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 156, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (150.54, 66.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.63, 87.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.305 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11810, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 121, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (155.93, 92.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.81, 85.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.460 CPU sec, 5.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11983, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 148, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (135.00, 85.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.64, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.202 CPU sec, 4.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11838, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 120, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (170.72, 135.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.07, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.982 CPU sec, 4.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11850, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 93, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (109.57, 49.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (190.54, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.094 CPU sec, 5.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11763, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 98, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (122.80, 72.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.49, 88.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.710 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11731, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 117, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (102.46, 48.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.07, 86.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.512 CPU sec, 5.089 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11755, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (118.73, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.72, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.592 CPU sec, 5.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11952, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (130.00, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.73, 87.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.472 CPU sec, 5.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11857, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 104, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (140.00, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.49, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.493 CPU sec, 5.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12146, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (129.06, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.48, 89.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.147 CPU sec, 4.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12087, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.21, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.295 CPU sec, 4.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12012, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (159.86, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.33, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.447 CPU sec, 5.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12054, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 125, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (121.47, 61.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.14, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.489 CPU sec, 5.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12036, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 92, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (111.32, 63.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.53, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.849 CPU sec, 6.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12078, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 133, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (119.33, 32.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.07, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.059 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12066, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 180, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (191.65, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.05, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.979 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11909, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 139, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (173.47, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.67, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.202 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11985, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (192.41, 89.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.941 CPU sec, 5.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12099, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 100, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (90.82, 42.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.19, 90.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.643 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12007, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 115, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (131.47, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (189.95, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.274 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12040, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 137, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (119.15, 51.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.91, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.908 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11960, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 127, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (120.64, 65.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.21, 86.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.684 CPU sec, 4.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11756, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 95, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (78.67, 39.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.38, 85.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.675 CPU sec, 5.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11818, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (149.69, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.80, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.897 CPU sec, 5.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11954, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 134, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (123.71, 71.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (189.31, 84.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.056 CPU sec, 5.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11826, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 151, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (170.83, 132.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.80, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.574 CPU sec, 6.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11664, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 82, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (104.36, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (187.31, 83.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.444 CPU sec, 5.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11804, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 123, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (147.65, 102.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.28, 84.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.131 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11779, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (154.12, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.61, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.488 CPU sec, 6.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11887, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 153, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (142.21, 74.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (189.94, 86.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.435 CPU sec, 5.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11823, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 178, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (145.67, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.80, 86.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.180 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11980, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 91, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (109.19, 59.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.17, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.541 CPU sec, 6.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12008, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 83, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (108.82, 78.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.32, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.469 CPU sec, 4.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12074, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (152.18, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.72, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.802 CPU sec, 6.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11918, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (180.07, 84.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.28, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.180 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12034, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (122.80, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.00, 89.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.161 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11946, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (148.76, 86.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.23, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.276 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11950, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 170, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (203.00, 148.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (195.10, 91.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.391 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12030, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (153.11, 72.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.28, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.662 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12011, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 78, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (81.79, 28.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.47, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.641 CPU sec, 6.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12155, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 125, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (155.69, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.66, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.348 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11773, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (143.75, 76.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.96, 87.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.645 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11818, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (147.08, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.69, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.123 CPU sec, 5.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11823, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 124, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (140.69, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.56, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.917 CPU sec, 5.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11863, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 96, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (107.85, 46.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.97, 86.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.302 CPU sec, 4.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11890, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 132, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (136.12, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.14, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.100 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11815, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.85, 85.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.312 CPU sec, 6.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11716, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 135, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (154.53, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.85, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.860 CPU sec, 4.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11633, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 116, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (124.82, 65.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.77, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.796 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11833, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 139, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (136.33, 76.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.50, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.346 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11754, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 134, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (131.07, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.64, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.950 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11884, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 163, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (156.17, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.90, 87.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.723 CPU sec, 5.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11753, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 169, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (156.06, 65.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.22, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.628 CPU sec, 6.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11952, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (135.76, 69.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.52, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.543 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12074, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 135, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (130.74, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.55, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.832 CPU sec, 5.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11925, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (116.47, 57.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.80, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.712 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11967, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 109, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (101.55, 46.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.26, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.441 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11874, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (119.88, 78.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.33, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.207 CPU sec, 4.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11848, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.42, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.775 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11860, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 171, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (160.94, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.49, 87.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.732 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11933, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 84, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (115.65, 72.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.13, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.088 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11829, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 66, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152) [M::mem_pestat] mean and std.dev: (62.56, 20.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (189.57, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.225 CPU sec, 5.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11946, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (161.62, 95.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.31, 83.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.108 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12023, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (127.76, 65.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.29, 83.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.656 CPU sec, 5.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11884, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 136, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (158.89, 105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.77, 85.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.185 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11832, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 141, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (130.10, 59.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (187.83, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.083 CPU sec, 6.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11701, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 92, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (100.21, 56.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.22, 86.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.408 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11992, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 101, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (136.46, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.77, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.553 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11795, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 149, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (155.29, 116.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.13, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.087 CPU sec, 5.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12075, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 119, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (139.93, 81.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.04, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.988 CPU sec, 5.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11909, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 119, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (111.20, 23.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.91, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.238 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11963, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (107.14, 51.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.31, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.288 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11950, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.35, 92.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.091 CPU sec, 5.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11887, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 158, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (144.00, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.13, 91.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.421 CPU sec, 4.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11916, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 101, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (121.74, 58.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.04, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.567 CPU sec, 4.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11987, 3, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.46, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.699 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11997, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 154, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (197.77, 143.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.59, 92.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.823 CPU sec, 5.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12067, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 113, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (125.47, 66.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (192.84, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.388 CPU sec, 6.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12005, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 81, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (73.05, 32.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.50, 90.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.979 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11857, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 157, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (129.15, 60.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.44, 91.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.097 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12042, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 148, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (173.75, 116.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.15, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.683 CPU sec, 5.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11877, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 133, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (153.25, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.36, 89.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.775 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11880, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 107, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (110.15, 43.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.24, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.306 CPU sec, 6.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11952, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 154, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (125.45, 62.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.41, 86.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.415 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11717, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 155, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (131.27, 71.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (188.62, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.745 CPU sec, 6.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11625, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 129, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (120.12, 52.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.98, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.362 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11720, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 104, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.21, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (185.78, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.051 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11391, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 122, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (148.00, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (187.46, 83.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.825 CPU sec, 5.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11924, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 108, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (118.75, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.56, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.690 CPU sec, 5.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11903, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 132, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (146.31, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.36, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.076 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12168, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.02, 92.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.330 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12035, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (124.64, 54.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.21, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.249 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12014, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 83, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (116.07, 76.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.80, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.902 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11957, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 109, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (114.42, 54.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.25, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.494 CPU sec, 5.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11986, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (204.33, 116.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.71, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.487 CPU sec, 5.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11881, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 98, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (116.69, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.32, 90.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.285 CPU sec, 5.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12130, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 159, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (135.30, 65.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.30, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.856 CPU sec, 5.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11913, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (148.45, 89.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.36, 93.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.627 CPU sec, 5.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12062, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 116, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 217) [M::mem_pestat] mean and std.dev: (107.73, 29.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.42, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.672 CPU sec, 6.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12095, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 103, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (125.21, 80.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.21, 90.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.012 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11933, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (133.85, 84.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.90, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.768 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12180, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 79, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157) [M::mem_pestat] mean and std.dev: (77.33, 32.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.55, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.539 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11970, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 99, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (106.40, 64.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.84, 87.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.519 CPU sec, 6.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11862, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 125, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (133.00, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (192.99, 89.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.686 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11854, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 93, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (109.57, 78.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (187.86, 83.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.268 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11492, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (142.85, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.32, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.933 CPU sec, 6.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11547, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 110, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (109.50, 30.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (186.23, 83.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.532 CPU sec, 5.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11215, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (173.06, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.99, 83.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.306 CPU sec, 5.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 151, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (200.53, 146.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.07, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.634 CPU sec, 5.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11755, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 90, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (97.64, 51.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (197.43, 93.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.728 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11837, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 141, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (131.73, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.13, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.451 CPU sec, 4.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11933, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (164.28, 107.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.45, 93.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.982 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11842, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 126, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (118.33, 38.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.35, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.975 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11931, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (104.00, 51.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.12, 93.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.771 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11968, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 101, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (106.76, 48.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.14, 93.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.916 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11976, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 162, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (210.80, 147.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.27, 92.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.938 CPU sec, 5.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11992, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 164, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (162.00, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.00, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.853 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12151, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (133.36, 65.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (194.93, 92.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.638 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12108, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 133, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (137.00, 78.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.10, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.564 CPU sec, 5.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11785, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 127, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (147.42, 121.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.82, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.938 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11912, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (139.29, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.67, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.935 CPU sec, 5.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12007, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 98, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (106.63, 59.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.51, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.494 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11989, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 98, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (84.94, 38.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.98, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.385 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11747, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 104, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (133.25, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.33, 83.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.158 CPU sec, 5.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11905, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (109.47, 82.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.90, 85.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.375 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11808, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 121, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (167.90, 105.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.00, 85.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.948 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11514, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (125.07, 57.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.27, 83.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.795 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11443, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 86, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (81.71, 28.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.66, 85.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.625 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11400, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 185, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (143.60, 79.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (184.27, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.272 CPU sec, 6.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11717, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 105, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (124.58, 100.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.02, 87.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.751 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11703, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (125.15, 62.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.63, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.089 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11811, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.25, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.147 CPU sec, 4.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12039, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 130, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.95, 81.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.95, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.009 CPU sec, 5.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11842, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 120, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (174.07, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.31, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.017 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11898, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 100, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (126.55, 59.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.58, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.613 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11568, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 165, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (166.81, 112.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.16, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.806 CPU sec, 5.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11929, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (133.53, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.65, 89.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.720 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11974, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 120, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (115.36, 74.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.70, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.403 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12002, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (120.17, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.43, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.873 CPU sec, 5.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11873, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 63, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (75.28, 39.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.60, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.773 CPU sec, 4.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11979, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.81, 89.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.557 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12014, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 180, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (220.82, 146.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.65, 87.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.788 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11971, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 149, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (168.23, 98.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.62, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.515 CPU sec, 5.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11916, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 81, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (75.08, 28.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.32, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.608 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11870, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (127.20, 63.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (186.88, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.633 CPU sec, 5.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11776, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (125.47, 67.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.43, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.606 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11792, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 121, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (126.33, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (184.90, 81.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.279 CPU sec, 5.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11704, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 120, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (100.89, 35.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (187.20, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.519 CPU sec, 4.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11453, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (123.65, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (184.54, 80.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.785 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11472, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 114, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (130.74, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (187.89, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.416 CPU sec, 5.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11798, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 142, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (136.38, 80.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.26, 91.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.291 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11889, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 152, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (140.64, 45.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.56, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.882 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11859, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 118, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (120.75, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.93, 89.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.961 CPU sec, 5.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11800, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (176.84, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.36, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.249 CPU sec, 5.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12028, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 72, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (68.43, 17.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.26, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.588 CPU sec, 5.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12067, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (164.25, 95.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.51, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.921 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11845, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 125, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (116.07, 60.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.45, 91.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.312 CPU sec, 5.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11973, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 111, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (124.08, 65.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.53, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.566 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12054, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (145.52, 78.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.37, 91.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.208 CPU sec, 5.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11946, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 166, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (161.71, 61.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.16, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.279 CPU sec, 6.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11961, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 130, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (137.35, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.83, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.059 CPU sec, 6.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11907, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (127.58, 79.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.17, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.622 CPU sec, 5.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12019, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 127, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (172.24, 108.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.03, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.501 CPU sec, 5.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11813, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (123.07, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.17, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.505 CPU sec, 5.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11861, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 118, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (118.91, 61.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.61, 85.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.037 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11871, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 125, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (135.17, 56.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.77, 85.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.301 CPU sec, 4.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11784, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (119.17, 63.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.54, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.077 CPU sec, 4.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11667, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 101, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (132.25, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (185.67, 81.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.917 CPU sec, 5.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11355, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 103, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (116.27, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 166, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (182.23, 79.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.783 CPU sec, 5.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11359, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 143, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (137.23, 61.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (184.57, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.448 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11682, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 132, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (148.55, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (193.85, 91.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.863 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11891, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (146.21, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.99, 90.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.680 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11971, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 120, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (145.94, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.60, 91.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.991 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11907, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (109.10, 48.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.22, 90.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.764 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11818, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 117, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (155.65, 102.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.85, 91.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.515 CPU sec, 5.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11885, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 103, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (120.65, 59.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.39, 87.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.772 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11955, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 126, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (138.12, 98.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.76, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.638 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11973, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 124, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (151.33, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.09, 89.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.910 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11901, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (146.19, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.28, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.130 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11893, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 143, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (158.77, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.73, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.208 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11908, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 91, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (114.09, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.25, 89.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.552 CPU sec, 5.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11876, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 147, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (143.60, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.03, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.104 CPU sec, 6.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11933, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 67, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (82.75, 48.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.64, 86.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.501 CPU sec, 5.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11952, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 110, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (101.36, 27.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 298) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.17, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.738 CPU sec, 5.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11823, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 142, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (135.92, 56.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.48, 87.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.763 CPU sec, 5.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11885, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 164, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (172.82, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.03, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.634 CPU sec, 5.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11994, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 110, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (112.23, 60.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.36, 85.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.736 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11664, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 111, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (150.22, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.34, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.063 CPU sec, 4.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11627, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (135.00, 74.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (184.85, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.826 CPU sec, 6.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11333, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (109.93, 66.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (182.65, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.476 CPU sec, 6.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11586, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (187.26, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.501 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11918, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 78, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (80.00, 26.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.99, 86.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.766 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12064, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 143, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (158.09, 95.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (189.97, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.722 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11856, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 144, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (137.80, 63.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (189.68, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.731 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11968, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (179.27, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (189.59, 87.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.980 CPU sec, 4.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11987, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (122.94, 66.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.48, 85.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.857 CPU sec, 4.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12029, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 103, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (101.94, 57.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.59, 87.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.196 CPU sec, 5.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12142, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 176, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (160.75, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.42, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.115 CPU sec, 4.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12058, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.38, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.757 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11939, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (172.06, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.24, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.632 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11987, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (111.19, 50.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.69, 89.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.025 CPU sec, 5.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11946, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 140, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (153.00, 105.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.63, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.921 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11848, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 100, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (90.95, 37.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.05, 87.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.814 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11860, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 154, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 277) [M::mem_pestat] mean and std.dev: (144.18, 43.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.73, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.410 CPU sec, 4.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11669, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.84, 87.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.308 CPU sec, 6.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11716, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (144.73, 81.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.76, 87.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.885 CPU sec, 6.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11820, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 84, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (95.50, 67.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.97, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.932 CPU sec, 5.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11933, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 125, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (148.17, 67.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.23, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.389 CPU sec, 5.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11821, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 95, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (110.00, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.14, 91.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.559 CPU sec, 5.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12121, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 106, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (117.20, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.19, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.943 CPU sec, 6.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11966, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 179, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (184.86, 112.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.51, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.840 CPU sec, 5.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11862, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 110, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (134.21, 81.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.18, 86.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.137 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11923, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 91, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (98.64, 62.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.02, 87.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.795 CPU sec, 4.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11891, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (123.00, 56.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.93, 86.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.168 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11901, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 109, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (119.45, 62.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.56, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.962 CPU sec, 7.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11864, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 121, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (166.21, 177.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.48, 84.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.741 CPU sec, 4.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11806, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 141, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (159.33, 118.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.45, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.256 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11882, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (128.85, 84.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.12, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.688 CPU sec, 5.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12092, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 114, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (148.38, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.21, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.696 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11843, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 98, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (113.50, 65.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.34, 90.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.842 CPU sec, 4.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12124, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 104, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (176.44, 117.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.48, 89.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.050 CPU sec, 4.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12141, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 88, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (146.17, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.63, 90.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.719 CPU sec, 5.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11946, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 180, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (179.56, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.25, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.234 CPU sec, 4.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11779, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.38, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.152 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11723, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 139, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (159.17, 111.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.84, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.631 CPU sec, 5.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11791, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 120, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (135.36, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.81, 85.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.158 CPU sec, 5.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12050, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 146, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (128.50, 48.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.99, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.441 CPU sec, 5.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11915, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 133, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (173.69, 132.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (192.50, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.785 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12050, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 136, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (140.31, 78.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.32, 87.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.957 CPU sec, 5.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12002, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (144.77, 124.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.99, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.942 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11852, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (111.43, 52.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.69, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.832 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11813, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 131, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (134.62, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.17, 85.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.158 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11977, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 176, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (168.55, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.99, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.545 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11962, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 100, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (108.00, 47.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.36, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.054 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11939, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 106, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (109.77, 57.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.10, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.916 CPU sec, 5.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11736, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (186.94, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.064 CPU sec, 5.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11973, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 104, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (121.58, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (191.58, 86.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.803 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12112, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 89, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (106.94, 50.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.20, 93.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.953 CPU sec, 4.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11923, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 141, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (152.43, 112.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.39, 90.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.968 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12042, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 116, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (110.60, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.74, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.963 CPU sec, 5.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12016, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 108, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (121.44, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.83, 90.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.277 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11913, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (129.33, 57.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.98, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.301 CPU sec, 5.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12028, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 95, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (89.70, 47.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.60, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.498 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11820, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 159, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (163.29, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.88, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.866 CPU sec, 5.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11707, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 143, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (161.93, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.74, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.186 CPU sec, 5.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11674, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (171.12, 146.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.96, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.021 CPU sec, 7.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11942, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 133, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (148.45, 87.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.14, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.005 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11861, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 177, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (175.69, 106.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.99, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.337 CPU sec, 4.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11960, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.43, 89.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.479 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11868, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (127.79, 90.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (194.68, 89.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.684 CPU sec, 6.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11907, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 161, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (160.93, 96.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.78, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.804 CPU sec, 5.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11832, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 134, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (151.00, 79.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.71, 86.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.703 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11811, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 138, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (170.00, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.42, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.680 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11850, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 72, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (93.81, 77.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.44, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.024 CPU sec, 4.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11826, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 206, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (193.50, 101.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (186.63, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.101 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12057, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 121, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (126.17, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.55, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.745 CPU sec, 5.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11873, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 110, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (122.72, 61.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (190.00, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.571 CPU sec, 5.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12133, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (117.55, 62.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (192.54, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.183 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11995, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.50, 81.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.51, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.409 CPU sec, 5.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11973, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 115, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (124.53, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.27, 87.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.198 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12023, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 127, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (139.06, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.79, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.890 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11801, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 109, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (110.50, 53.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.78, 87.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.364 CPU sec, 5.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11882, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 78, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (93.67, 36.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.18, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.416 CPU sec, 5.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11726, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (119.87, 58.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (185.15, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.459 CPU sec, 5.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11558, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (163.10, 106.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (186.74, 83.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.987 CPU sec, 5.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11643, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 108, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (128.71, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (189.62, 87.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.545 CPU sec, 5.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11948, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.74, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.072 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11895, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (142.71, 83.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (189.94, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.931 CPU sec, 6.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12042, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 108, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (111.88, 71.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.03, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.906 CPU sec, 4.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11810, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 110, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (106.08, 50.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.64, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.768 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12001, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (120.76, 62.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.66, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.615 CPU sec, 5.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12007, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 94, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (113.25, 63.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (188.96, 86.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.336 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11924, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 130, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (129.08, 59.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (189.35, 85.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.532 CPU sec, 5.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11840, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (120.62, 59.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.99, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.560 CPU sec, 6.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11810, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 151, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (136.36, 55.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (187.14, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.831 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11807, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (210.55, 194.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.26, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.616 CPU sec, 5.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11940, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 154, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (180.20, 164.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.39, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.208 CPU sec, 5.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12149, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 93, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (120.29, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.38, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.553 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11941, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 111, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (114.50, 60.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.41, 87.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.718 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12077, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 186, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (176.83, 82.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.80, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.782 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12100, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 131, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (135.00, 39.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.96, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.856 CPU sec, 5.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11894, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 144, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (131.43, 48.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.74, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.442 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11961, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (115.33, 74.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.00, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.892 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11655, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 158, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (105.91, 55.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (188.09, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.273 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11724, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 165, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (157.05, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (187.40, 83.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.022 CPU sec, 5.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11748, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 191, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (159.88, 78.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (187.23, 83.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.457 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11871, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 101, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (103.82, 68.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.33, 85.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.024 CPU sec, 5.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11887, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 128, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (144.25, 53.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.41, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.102 CPU sec, 4.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11842, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 98, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (103.94, 67.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.61, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.359 CPU sec, 6.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12009, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 153, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (156.16, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.17, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.795 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11990, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (148.43, 99.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.32, 87.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.449 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11743, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 135, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (154.69, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.09, 84.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.354 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11864, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (128.05, 65.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.92, 84.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.278 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11841, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 140, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (140.50, 76.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (188.30, 85.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.071 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11864, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 155, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (164.21, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (185.21, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.121 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11897, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 153, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (163.50, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (186.21, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.742 CPU sec, 5.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11756, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 135, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 209) [M::mem_pestat] mean and std.dev: (128.53, 31.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.72, 85.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.035 CPU sec, 5.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11850, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 139, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (143.94, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.44, 89.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.085 CPU sec, 5.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11843, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 100, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (99.62, 49.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.08, 86.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.565 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12047, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (142.14, 80.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.47, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.651 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11871, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 107, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.23, 69.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.34, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.866 CPU sec, 5.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12031, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 123, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (135.00, 64.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.26, 87.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.690 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11898, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 111, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (152.30, 105.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (188.98, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.566 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11665, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 139, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (127.80, 50.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.37, 85.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.935 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11710, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (115.24, 61.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.39, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.337 CPU sec, 5.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11757, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 116, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (105.12, 41.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (188.89, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.564 CPU sec, 5.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11925, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 93, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (106.33, 50.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.11, 87.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.267 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11946, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 138, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (147.30, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.70, 87.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.476 CPU sec, 5.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11967, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 131, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (151.82, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.23, 86.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.535 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11990, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 157, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (147.61, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.97, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.720 CPU sec, 6.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11903, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 97, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (93.07, 40.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.67, 87.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.343 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11746, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (121.77, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.93, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.562 CPU sec, 6.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11805, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 98, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (148.94, 116.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (185.71, 83.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.522 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11677, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 163, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (180.61, 105.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (186.19, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.374 CPU sec, 4.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11637, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 117, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 219) [M::mem_pestat] mean and std.dev: (99.93, 30.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.81, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.495 CPU sec, 5.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11396, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 140, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (184.28, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.52, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.728 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11508, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 88, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (124.67, 80.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.10, 87.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.881 CPU sec, 7.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11946, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 163, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (166.63, 102.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.48, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.927 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11976, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (165.10, 121.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.68, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.043 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11926, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 103, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (110.89, 47.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.63, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.330 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11826, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 97, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (140.67, 115.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.76, 87.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.549 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11890, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 107, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (138.23, 105.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.58, 87.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.432 CPU sec, 5.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12060, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 95, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (138.20, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.46, 86.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.561 CPU sec, 5.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12049, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 102, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (110.77, 56.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.56, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.145 CPU sec, 4.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11945, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 115, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (116.50, 65.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.31, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.202 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11798, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 83, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (85.40, 24.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.38, 91.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.575 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12116, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 94, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (105.19, 53.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.04, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.547 CPU sec, 5.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11983, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 126, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (176.77, 145.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.22, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.260 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11843, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 123, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (139.75, 81.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.48, 90.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.754 CPU sec, 5.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12067, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 112, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (119.69, 76.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.37, 88.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.253 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12040, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 141, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (145.52, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.06, 89.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.391 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11881, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 138, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (122.59, 61.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.51, 85.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.745 CPU sec, 5.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11660, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 163, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (163.13, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.54, 85.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.491 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11710, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 131, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (138.00, 68.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.41, 84.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.595 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11620, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 125, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (152.41, 90.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (187.31, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.625 CPU sec, 5.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11382, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 142, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (150.62, 87.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.43, 85.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.704 CPU sec, 6.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11550, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (122.56, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.53, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.807 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11987, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 103, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (117.21, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (196.69, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.788 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12042, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 138, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (168.17, 99.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.67, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.388 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11955, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 163, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (184.29, 166.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.57, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.984 CPU sec, 5.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11936, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 145, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (156.93, 88.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.64, 88.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.762 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11974, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 123, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (147.08, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.65, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.818 CPU sec, 5.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11951, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 127, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (120.46, 46.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.94, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.256 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11831, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 86, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (134.75, 112.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.14, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.490 CPU sec, 4.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11838, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 167, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (205.00, 112.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (196.68, 92.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.977 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12049, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 137, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (135.50, 54.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.60, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.295 CPU sec, 5.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11996, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 198, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (177.33, 98.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.52, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.630 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11983, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (126.43, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.04, 93.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.235 CPU sec, 6.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12016, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.59, 89.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.322 CPU sec, 5.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12033, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (130.07, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.35, 92.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.941 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11850, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 146, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (152.92, 78.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.88, 88.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.558 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11879, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 132, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (131.65, 85.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.85, 84.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.619 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11858, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 125, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (162.76, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.40, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.418 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11520, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 102, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (100.93, 46.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.25, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.266 CPU sec, 6.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11750, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 114, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (133.92, 86.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (187.87, 84.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.810 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11577, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (149.20, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.84, 85.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.834 CPU sec, 4.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11742, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 171, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (172.72, 109.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.02, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.642 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12043, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 142, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (160.73, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.59, 88.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.325 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11895, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 77, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (109.36, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.09, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.484 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12002, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 159, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (156.94, 107.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.42, 90.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.904 CPU sec, 5.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12115, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 133, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (172.77, 102.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.13, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.401 CPU sec, 5.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12077, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (109.28, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.95, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.440 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11903, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 103, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (161.31, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.57, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.805 CPU sec, 5.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11987, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 97, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (161.64, 129.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.37, 89.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.424 CPU sec, 4.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12013, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 131, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (150.62, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.65, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.000 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12059, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 159, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (166.40, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.05, 86.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.693 CPU sec, 6.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12211, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 114, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (94.47, 64.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.23, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.498 CPU sec, 4.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12033, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 110, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (113.75, 72.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.87, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.910 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11924, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (127.93, 88.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.36, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.476 CPU sec, 5.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11882, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (195.29, 124.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.26, 90.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.960 CPU sec, 4.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11980, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (136.08, 86.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (188.34, 85.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.003 CPU sec, 6.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11924, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 117, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (142.08, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.72, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.711 CPU sec, 5.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11705, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 95, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (92.56, 48.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (185.45, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.382 CPU sec, 4.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11672, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 93, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (84.18, 46.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (183.48, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.933 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11539, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (162.00, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 164, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (183.38, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.820 CPU sec, 4.747 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11397, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 142, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (118.06, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 165, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (182.57, 78.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.989 CPU sec, 6.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11721, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 113, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (155.33, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.72, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.993 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12109, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 98, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (96.19, 38.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (193.24, 89.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.430 CPU sec, 4.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11958, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 152, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (158.67, 72.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.85, 88.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.928 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12011, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 138, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (138.00, 85.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.45, 87.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.489 CPU sec, 5.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12049, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 121, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (136.24, 77.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.16, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.253 CPU sec, 4.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12030, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (161.53, 108.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.70, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.255 CPU sec, 4.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11813, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 110, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (128.87, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.56, 89.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.835 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11967, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 109, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (124.89, 78.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.08, 89.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.040 CPU sec, 5.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11935, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 190, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (174.92, 85.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.36, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.604 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11939, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 96, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (107.00, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.70, 89.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.954 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11863, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 95, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (103.06, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.71, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.143 CPU sec, 6.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12095, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 159, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (152.71, 55.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.45, 89.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.094 CPU sec, 4.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11742, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 118, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (125.95, 84.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.24, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.187 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11953, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 184, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (168.21, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.04, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.318 CPU sec, 4.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11886, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 118, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (111.78, 57.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (185.37, 81.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.404 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11877, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (139.76, 58.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.46, 85.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.672 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11604, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (151.41, 71.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (185.40, 82.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.405 CPU sec, 5.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11610, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 107, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (124.81, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (185.59, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.287 CPU sec, 5.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11442, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (159.14, 103.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 165, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (182.95, 78.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.143 CPU sec, 5.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11522, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 164, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (182.18, 77.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.769 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11722, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (121.00, 66.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.02, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.073 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12075, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 147, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (150.36, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.95, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.525 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11961, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 206, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (149.91, 104.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.82, 87.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.826 CPU sec, 4.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12026, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 97, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (101.75, 42.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.22, 88.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.925 CPU sec, 4.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12108, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (121.65, 66.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.85, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.856 CPU sec, 5.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11961, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 93, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (124.90, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.60, 90.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.990 CPU sec, 4.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11894, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (125.70, 64.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.39, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.028 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12055, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 266, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (213.27, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.58, 86.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.572 CPU sec, 4.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11870, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (191.19, 88.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.335 CPU sec, 4.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12180, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 121, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (132.27, 48.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.53, 88.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.181 CPU sec, 5.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11919, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 119, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (138.94, 80.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.49, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.867 CPU sec, 4.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11832, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (113.00, 55.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.71, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.750 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11919, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 167, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (187.00, 115.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.02, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.401 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11954, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 96, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (104.92, 62.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.44, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.128 CPU sec, 6.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12040, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.98, 87.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.101 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11928, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (124.36, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.96, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.577 CPU sec, 5.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11934, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 116, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (117.50, 53.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (188.08, 83.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.966 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11839, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 119, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (141.00, 100.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (185.64, 81.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.051 CPU sec, 4.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11724, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 160, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (156.08, 83.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 165, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (184.57, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.391 CPU sec, 5.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11646, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 136, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (132.93, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (185.00, 80.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.027 CPU sec, 7.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11632, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 150, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (127.75, 68.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.25, 85.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.707 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11859, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 151, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (180.50, 114.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.47, 87.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.291 CPU sec, 6.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11899, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 121, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (132.39, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.31, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.041 CPU sec, 5.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11926, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (117.60, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.08, 89.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.163 CPU sec, 4.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12013, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.10, 86.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.945 CPU sec, 5.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12065, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 112, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (113.50, 62.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.32, 86.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.380 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11875, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 65, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (95.30, 63.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.92, 87.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.952 CPU sec, 5.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11955, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 162, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (163.41, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.18, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.263 CPU sec, 6.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12010, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 163, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (148.75, 72.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.98, 87.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.537 CPU sec, 6.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11954, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 98, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (106.58, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.48, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.090 CPU sec, 5.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12070, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 109, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (152.18, 83.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.23, 87.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.177 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12027, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 124, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (148.11, 71.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.69, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.275 CPU sec, 5.547 real sec [M::process] read 63146 sequences (9535046 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11802, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (134.88, 82.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.17, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.868 CPU sec, 5.134 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11288, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 129, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (111.07, 61.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.78, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 63146 reads in 141.698 CPU sec, 5.811 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21162\tSM:UG10S1P021\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21162_HLKNYBGXX_UG10S1P021_R1.fastq.gz 5347_7332_21162_HLKNYBGXX_UG10S1P021_R2.fastq.gz [main] Real time: 2633.988 sec; CPU: 68384.027 sec Finished alignement of UG10S1P021 at Wed Apr 3 06:30:17 2019 Sorting BAM file started at Wed Apr 3 06:30:17 2019 samtools sort -@ 30 -m 4G UG10S1P021_unsorted.bam -o UG10S1P021.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 06:30:17 2019 rm UG10S1P021_unsorted.bam rm 5347_7332_21162_HLKNYBGXX_UG10S1P021_R1.fastq.gz rm 5347_7332_21162_HLKNYBGXX_UG10S1P021_R2.fastq.gz samtools index UG10S1P021.bam Finished BAM file generation of the sample - UG10S1P021 Processing fastq files for the sample - UG10S2P153 Reading 5347_7332_21163_HLKNYBGXX_UG10S2P153_R1.fastq.gz 5347_7332_21163_HLKNYBGXX_UG10S2P153_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21163\tSM:UG10S2P153\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21163_HLKNYBGXX_UG10S2P153_R1.fastq.gz 5347_7332_21163_HLKNYBGXX_UG10S2P153_R2.fastq.gz | samtools view -Sb1 - > UG10S2P153_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12446, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.08, 47.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.612 CPU sec, 5.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12490, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 62, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (104.58, 117.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.51, 47.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.525 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12617, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.21, 48.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.491 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12554, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (152.36, 97.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.79, 49.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.722 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12653, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 96, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (132.75, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.70, 48.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.987 CPU sec, 5.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12698, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (98.78, 27.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.49, 47.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.829 CPU sec, 5.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12565, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 104, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (107.92, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (128.44, 48.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.830 CPU sec, 5.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12731, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.10, 47.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.638 CPU sec, 5.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12674, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 120, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (118.07, 60.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.32, 46.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.578 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12436, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 134, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (158.50, 129.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (129.26, 47.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.850 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12484, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 64, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (75.75, 51.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.80, 48.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.322 CPU sec, 5.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12528, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 88, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (101.24, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.70, 48.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.142 CPU sec, 6.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12650, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.17, 49.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.567 CPU sec, 4.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12635, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (129.94, 47.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.512 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12699, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.77, 48.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.333 CPU sec, 5.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12500, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 92, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (92.50, 45.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.07, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.704 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12654, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.91, 46.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.481 CPU sec, 5.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12699, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 102, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (103.15, 52.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.33, 46.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.899 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12609, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.80, 46.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.713 CPU sec, 5.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12291, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (130.10, 47.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.081 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12505, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (115.77, 74.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.16, 49.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.052 CPU sec, 7.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12693, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.46, 47.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.330 CPU sec, 5.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12721, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 108, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (104.44, 44.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.65, 49.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.199 CPU sec, 5.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12648, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.02, 47.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.373 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12442, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.45, 47.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.357 CPU sec, 6.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12515, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 101, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (156.27, 110.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.79, 47.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.176 CPU sec, 6.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12513, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 90, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (78.38, 36.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.10, 46.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.861 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12551, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 98, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (127.27, 92.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (127.97, 46.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.698 CPU sec, 5.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12418, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.66, 47.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.483 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12653, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (19, 80, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (74.00, 65.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.10, 46.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.554 CPU sec, 4.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12751, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.02, 48.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.891 CPU sec, 5.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12583, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 127, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (172.30, 145.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.45, 47.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.893 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12690, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.00, 48.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.081 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12586, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 128, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (105.20, 40.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (127.87, 46.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.609 CPU sec, 5.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12612, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.36, 46.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.110 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12533, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.96, 46.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.353 CPU sec, 5.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12502, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 59, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (73.21, 36.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.75, 46.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.005 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12406, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 104, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (115.60, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (128.28, 45.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.071 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12286, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.05, 46.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.789 CPU sec, 6.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12510, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 137, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (208.73, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.65, 47.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.262 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12730, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.11, 46.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.163 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12613, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 167, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (168.60, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.36, 47.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.590 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12653, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 155, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (135.44, 60.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.21, 48.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.170 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12716, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 89, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (116.00, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.55, 47.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.981 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12675, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.58, 47.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.188 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12609, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 88, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (123.54, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.54, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.363 CPU sec, 6.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12659, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 121, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (114.77, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.44, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.833 CPU sec, 4.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12656, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 89, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (111.42, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.68, 46.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.748 CPU sec, 5.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12365, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 72, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (96.73, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.78, 47.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.139 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12584, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.24, 47.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.833 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12663, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (100.33, 49.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.66, 48.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.807 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12538, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 99, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (101.46, 50.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.48, 47.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.526 CPU sec, 4.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12633, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 167, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (165.69, 81.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.39, 47.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.129 CPU sec, 5.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12580, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 81, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (101.15, 69.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (127.66, 46.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.360 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12505, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.74, 46.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.008 CPU sec, 5.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12506, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 93, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (108.46, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.44, 46.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.323 CPU sec, 5.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12697, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.08, 46.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.618 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12465, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.72, 46.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.531 CPU sec, 5.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12653, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 142, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (155.55, 69.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (130.17, 48.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.056 CPU sec, 4.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12461, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.15, 48.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.921 CPU sec, 5.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12473, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (130.02, 48.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.164 CPU sec, 4.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12549, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.22, 47.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.510 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12587, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.57, 48.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.823 CPU sec, 5.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12497, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 70, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (80.75, 48.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.23, 47.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.660 CPU sec, 5.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12414, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.39, 46.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.202 CPU sec, 5.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12498, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 141, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 228) [M::mem_pestat] mean and std.dev: (124.10, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.26, 46.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.671 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12120, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (128.04, 45.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.021 CPU sec, 5.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12555, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (131.75, 49.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.707 CPU sec, 5.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12494, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 234, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (180.89, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.41, 48.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.316 CPU sec, 6.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12473, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 122, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (118.54, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (130.49, 48.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.530 CPU sec, 4.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12596, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 83, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (83.50, 58.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (130.56, 49.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.697 CPU sec, 5.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12477, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 88, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (92.86, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.12, 48.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.561 CPU sec, 5.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12489, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 95, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (113.33, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (130.10, 47.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.870 CPU sec, 5.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12566, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (127.87, 45.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.605 CPU sec, 5.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12730, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 137, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (112.70, 56.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.18, 45.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.728 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12352, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 156, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (148.60, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (126.98, 45.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.473 CPU sec, 5.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12489, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.57, 47.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.768 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12414, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (130.93, 48.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.286 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12523, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 73, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (93.50, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (130.38, 47.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.345 CPU sec, 5.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12587, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 120, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (118.42, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.87, 47.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.133 CPU sec, 5.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12465, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (129.79, 48.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.534 CPU sec, 5.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12582, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 57, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (54.00, 30.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.66, 47.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.375 CPU sec, 6.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12521, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.13, 47.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.041 CPU sec, 5.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12657, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 73, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (101.55, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.29, 45.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.617 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12450, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 92, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (111.25, 104.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.32, 45.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.166 CPU sec, 6.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12399, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (127.69, 45.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.235 CPU sec, 5.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12522, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 82, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (75.33, 34.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.55, 47.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.143 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12551, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 93, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (96.92, 39.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.62, 47.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.682 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12611, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 147, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (143.30, 69.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.68, 48.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.667 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12424, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.28, 48.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.257 CPU sec, 6.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12635, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.74, 47.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.701 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12438, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 84, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (88.50, 38.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.79, 46.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.811 CPU sec, 5.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12587, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 93, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (78.00, 41.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.63, 45.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.609 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12522, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (129.20, 46.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.241 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12323, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.19, 46.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.251 CPU sec, 4.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12349, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 92, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (87.88, 31.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.98, 46.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.016 CPU sec, 5.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12539, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.08, 48.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.301 CPU sec, 5.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12589, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.43, 48.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.437 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12582, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (129.81, 48.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.818 CPU sec, 5.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12454, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.41, 49.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.293 CPU sec, 5.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12800, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.04, 46.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.985 CPU sec, 5.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12641, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (127.93, 46.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.428 CPU sec, 4.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12501, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 73, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (66.64, 27.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (127.66, 45.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.212 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12501, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (127.90, 46.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.193 CPU sec, 4.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12271, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (127.37, 45.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.376 CPU sec, 5.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12359, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 75, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (121.85, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.17, 47.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.480 CPU sec, 5.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12513, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 178, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (174.40, 137.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.14, 47.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.662 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12447, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 128, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (132.09, 74.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.92, 47.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.511 CPU sec, 5.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12569, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (128.92, 47.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.959 CPU sec, 5.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12591, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (166.00, 149.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.43, 46.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.230 CPU sec, 5.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12587, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 60, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (62.33, 27.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.53, 47.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.464 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12583, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 66, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (71.55, 33.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.58, 46.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.614 CPU sec, 4.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12509, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 79, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (70.12, 35.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.32, 45.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.014 CPU sec, 4.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12243, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (126.65, 44.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.719 CPU sec, 5.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12459, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 83, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (73.67, 31.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.69, 45.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.266 CPU sec, 5.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12652, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 96, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (132.91, 105.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.47, 46.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.517 CPU sec, 5.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12903, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 137, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (132.17, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.07, 46.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.259 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12487, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 83, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 154) [M::mem_pestat] mean and std.dev: (82.14, 31.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (129.69, 47.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.078 CPU sec, 4.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12503, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.46, 46.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.094 CPU sec, 4.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12621, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 147, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (143.00, 58.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.28, 46.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.499 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12465, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 84, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (76.15, 34.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.37, 46.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.915 CPU sec, 5.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12535, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 106, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (107.60, 65.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.71, 47.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.425 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12616, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.90, 47.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.160 CPU sec, 5.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12549, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.77, 48.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.646 CPU sec, 5.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12702, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 146, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (138.00, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.95, 46.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.886 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12522, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (129.47, 46.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.225 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12684, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.95, 46.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.404 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12630, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.34, 47.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.078 CPU sec, 4.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12705, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (72.18, 43.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.70, 47.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.274 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12577, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.47, 48.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.677 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12377, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 150, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (149.53, 50.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.31, 47.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.268 CPU sec, 5.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12620, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (130.65, 48.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.666 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12741, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.34, 48.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.777 CPU sec, 4.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12690, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.95, 46.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.248 CPU sec, 4.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12656, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (127.18, 45.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.830 CPU sec, 5.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12491, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.18, 46.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.915 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12407, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (129.46, 47.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.381 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12632, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 91, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (82.78, 38.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.71, 48.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.807 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12432, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.14, 46.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.417 CPU sec, 5.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12510, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 101, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (111.40, 55.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.40, 46.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.582 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12726, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.30, 47.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.984 CPU sec, 5.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12613, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 117, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 213) [M::mem_pestat] mean and std.dev: (108.07, 44.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (130.07, 48.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.470 CPU sec, 5.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12675, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 75, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (76.46, 51.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (128.04, 46.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.537 CPU sec, 5.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12631, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 104, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (120.09, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (127.92, 45.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.347 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12749, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 136, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 280) [M::mem_pestat] mean and std.dev: (133.69, 49.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.19, 47.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.532 CPU sec, 6.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12377, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.14, 48.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.648 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12623, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 158, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (147.38, 40.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.88, 46.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.373 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12695, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.69, 46.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.785 CPU sec, 4.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12429, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.12, 45.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.526 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12562, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (29, 88, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (81.91, 68.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.09, 45.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.383 CPU sec, 5.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12641, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (21, 107, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (128.14, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.58, 47.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.926 CPU sec, 4.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12677, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (31, 70, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (80.60, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.46, 46.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.973 CPU sec, 4.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12705, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (91.75, 39.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.64, 46.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.954 CPU sec, 5.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12683, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (132.90, 81.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (129.22, 46.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.759 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12694, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 159, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (146.76, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.66, 46.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.133 CPU sec, 5.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12613, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.56, 47.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.971 CPU sec, 6.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12727, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.68, 46.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.336 CPU sec, 5.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12446, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (130.32, 48.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.598 CPU sec, 4.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12563, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (127.89, 46.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.020 CPU sec, 5.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12584, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 67, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (88.00, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.55, 46.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.470 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12651, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.72, 48.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.874 CPU sec, 5.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12892, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 104, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.36, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (127.08, 45.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.383 CPU sec, 5.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12572, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (127.24, 45.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.956 CPU sec, 5.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12671, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (104.50, 50.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.40, 47.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.672 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12679, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 143, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (153.10, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (129.15, 46.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.577 CPU sec, 5.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12723, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 121, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (119.89, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.73, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.529 CPU sec, 4.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12496, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.54, 46.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.754 CPU sec, 6.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12500, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (31, 80, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (80.33, 67.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (128.84, 45.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.021 CPU sec, 5.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12626, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (106.30, 54.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.38, 47.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.345 CPU sec, 5.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12650, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 104, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (112.80, 61.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (130.02, 47.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.224 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12426, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (154.33, 84.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.34, 45.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.960 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12396, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 103, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (102.89, 47.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (128.04, 45.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.473 CPU sec, 5.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12357, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (129.42, 46.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.305 CPU sec, 4.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12724, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (126.31, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.49, 47.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.284 CPU sec, 4.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12742, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 82, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (112.09, 91.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.55, 48.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.174 CPU sec, 6.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12748, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.78, 47.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.712 CPU sec, 5.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12523, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (129.55, 47.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.546 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12614, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 85, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (79.25, 19.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (129.56, 47.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.465 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12622, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.78, 48.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.585 CPU sec, 5.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12650, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 116, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (115.17, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (127.32, 44.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.456 CPU sec, 5.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12522, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (126.80, 45.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.620 CPU sec, 5.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12229, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.77, 46.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.679 CPU sec, 5.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12523, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 93, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (99.15, 50.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (130.80, 49.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.264 CPU sec, 5.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12632, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 84, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (93.21, 57.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.21, 47.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.477 CPU sec, 4.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12501, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 160, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (160.67, 89.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.01, 47.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.574 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12699, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 140, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (119.27, 62.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (129.24, 46.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.236 CPU sec, 4.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12653, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (130.36, 47.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.951 CPU sec, 4.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12660, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (129.41, 48.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.232 CPU sec, 6.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12895, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.53, 46.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.532 CPU sec, 5.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 12612, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (129.10, 46.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.231 CPU sec, 6.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12275, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (127.97, 45.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.921 CPU sec, 5.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12505, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 102, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (109.08, 62.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (129.65, 47.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.260 CPU sec, 5.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12649, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 107, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (101.78, 45.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (129.92, 48.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.589 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12665, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 105, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (140.92, 108.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.95, 46.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.446 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12542, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 100, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (128.38, 93.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.15, 47.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.994 CPU sec, 5.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12692, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 102, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (112.40, 49.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.48, 48.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.185 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12697, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 61, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (83.93, 53.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.49, 47.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.601 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12619, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 77, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (65.75, 21.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.42, 46.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.268 CPU sec, 5.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12609, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.50, 46.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.577 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12324, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.96, 45.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.805 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12557, 0, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.16, 47.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.100 CPU sec, 6.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12664, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 84, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (85.15, 36.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.66, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.880 CPU sec, 4.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12549, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 77, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (109.55, 63.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.83, 47.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.197 CPU sec, 5.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12740, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (130.05, 47.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.926 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12758, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.70, 46.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.295 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12494, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 100, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (114.20, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (129.07, 47.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.547 CPU sec, 5.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12589, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 72, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 122) [M::mem_pestat] mean and std.dev: (67.92, 14.50) [M::mem_pestat] low and high boundaries for proper pairs: (2, 142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.82, 47.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.038 CPU sec, 5.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12572, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 88, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (89.92, 34.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (127.22, 45.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.195 CPU sec, 5.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12279, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 122, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (126.34, 44.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.835 CPU sec, 5.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12201, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.47, 46.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.334 CPU sec, 5.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12634, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (128.76, 47.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.748 CPU sec, 4.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12749, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.91, 47.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.746 CPU sec, 5.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12570, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.64, 47.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.910 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12399, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.59, 47.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.782 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12600, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (131.03, 48.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.977 CPU sec, 5.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12770, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 88, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (89.91, 65.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.70, 48.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.626 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12543, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 152, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (148.00, 61.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.60, 47.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.672 CPU sec, 4.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 12539, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (126.96, 45.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.785 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12312, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (126.91, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.355 CPU sec, 5.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12584, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 137, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (142.86, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (130.83, 48.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.837 CPU sec, 5.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12641, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 99, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (104.91, 32.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (128.36, 46.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.193 CPU sec, 5.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12682, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.08, 46.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.165 CPU sec, 6.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12515, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 55, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (99.09, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (127.80, 46.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.959 CPU sec, 5.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12525, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (128.64, 47.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.264 CPU sec, 4.861 real sec [M::process] read 1446 sequences (218346 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12713, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (99, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (129.38, 47.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.279 CPU sec, 4.816 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (128.14, 49.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 1446 reads in 3.191 CPU sec, 0.296 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21163\tSM:UG10S2P153\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21163_HLKNYBGXX_UG10S2P153_R1.fastq.gz 5347_7332_21163_HLKNYBGXX_UG10S2P153_R2.fastq.gz [main] Real time: 1202.372 sec; CPU: 31672.658 sec Finished alignement of UG10S2P153 at Wed Apr 3 07:16:34 2019 Sorting BAM file started at Wed Apr 3 07:16:34 2019 samtools sort -@ 30 -m 4G UG10S2P153_unsorted.bam -o UG10S2P153.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 07:16:34 2019 rm UG10S2P153_unsorted.bam rm 5347_7332_21163_HLKNYBGXX_UG10S2P153_R1.fastq.gz rm 5347_7332_21163_HLKNYBGXX_UG10S2P153_R2.fastq.gz samtools index UG10S2P153.bam Finished BAM file generation of the sample - UG10S2P153 Processing fastq files for the sample - UG10S2P154 Reading 5347_7332_21164_HLKNYBGXX_UG10S2P154_R1.fastq.gz 5347_7332_21164_HLKNYBGXX_UG10S2P154_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21164\tSM:UG10S2P154\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21164_HLKNYBGXX_UG10S2P154_R1.fastq.gz 5347_7332_21164_HLKNYBGXX_UG10S2P154_R2.fastq.gz | samtools view -Sb1 - > UG10S2P154_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12050, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 131, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (139.91, 76.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.33, 68.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.964 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12156, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 90, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (125.47, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (159.45, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.524 CPU sec, 4.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12354, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 100, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (124.00, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.51, 69.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.240 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12325, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 122, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (130.38, 68.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.48, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.511 CPU sec, 6.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12535, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 75, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (128.12, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (162.11, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.524 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12441, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 96, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (120.79, 66.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.09, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.717 CPU sec, 5.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12364, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 137, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (130.06, 56.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (162.10, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.538 CPU sec, 5.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12491, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 130, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (144.31, 77.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.46, 69.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.792 CPU sec, 4.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12286, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 71, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 142) [M::mem_pestat] mean and std.dev: (72.67, 30.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.41, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.990 CPU sec, 4.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12357, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (116.94, 74.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.31, 67.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.287 CPU sec, 5.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12467, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 111, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (140.92, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.49, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.596 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12435, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 124, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (123.06, 59.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.43, 65.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.630 CPU sec, 6.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12342, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 118, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (119.65, 45.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.72, 66.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.578 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12281, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 133, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (137.56, 89.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.12, 65.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.333 CPU sec, 4.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12432, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (170.63, 116.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (159.29, 66.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.512 CPU sec, 7.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12129, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (114.64, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.35, 67.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.029 CPU sec, 5.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12146, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (130.92, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.41, 67.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.938 CPU sec, 5.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12382, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (104.08, 37.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.79, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.078 CPU sec, 6.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12390, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.34, 68.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.491 CPU sec, 5.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12298, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (125.55, 79.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (161.10, 68.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.413 CPU sec, 5.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12265, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 113, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (128.43, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (161.37, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.573 CPU sec, 5.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12326, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.26, 67.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 125.157 CPU sec, 4.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12314, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 120, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (129.39, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (163.47, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.294 CPU sec, 4.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12410, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 221, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (180.92, 108.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.38, 67.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.500 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12364, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 101, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (127.48, 82.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.04, 68.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.981 CPU sec, 4.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12450, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 88, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (104.57, 66.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.40, 68.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.727 CPU sec, 5.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12511, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 110, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (106.38, 62.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.20, 66.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.188 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12421, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 111, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (88.00, 42.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.19, 66.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.971 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12476, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 75, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (92.18, 56.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.80, 66.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.486 CPU sec, 4.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12373, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 101, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (99.62, 38.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.65, 67.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.266 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11936, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (107.58, 57.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.51, 66.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.635 CPU sec, 4.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12234, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 175, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (149.55, 121.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.98, 66.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.257 CPU sec, 4.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12203, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 160, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (149.65, 64.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.13, 67.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.077 CPU sec, 5.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12228, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 88, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (97.62, 56.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.40, 68.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.241 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12180, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 152, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (171.12, 109.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.13, 67.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.878 CPU sec, 4.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12511, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 130, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (132.36, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.40, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.426 CPU sec, 4.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12282, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (169.11, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.44, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.585 CPU sec, 5.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12409, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (119.35, 50.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.51, 65.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.102 CPU sec, 5.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12288, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (122.28, 59.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.95, 67.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.948 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12381, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (137.64, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (160.23, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.682 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12395, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 81, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (101.85, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.21, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.162 CPU sec, 4.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12393, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 109, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (104.69, 45.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.53, 66.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.520 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12421, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 86, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (88.60, 44.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.81, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.867 CPU sec, 5.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12447, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (127.35, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.29, 67.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.661 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12416, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (139.67, 92.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.62, 66.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.389 CPU sec, 4.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12133, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 87, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (106.46, 52.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.30, 66.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.594 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12333, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 107, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (89.31, 40.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (158.33, 64.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.797 CPU sec, 5.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12126, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 82, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (99.33, 60.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.60, 67.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.108 CPU sec, 4.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12499, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 119, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (125.21, 53.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.06, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.924 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12464, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 92, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (102.05, 47.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.99, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.257 CPU sec, 6.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12482, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 109, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (124.00, 83.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.90, 67.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.266 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12488, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 77, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 151) [M::mem_pestat] mean and std.dev: (69.08, 25.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.00, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.775 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12433, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (153.17, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.35, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.102 CPU sec, 4.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12287, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 136, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (119.33, 51.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.90, 66.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.684 CPU sec, 5.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12372, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 131, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (122.77, 65.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.34, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.623 CPU sec, 4.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12398, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (140.06, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.74, 66.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.315 CPU sec, 4.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12299, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (100.60, 43.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (159.83, 66.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.496 CPU sec, 6.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12422, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 86, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (115.48, 66.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.49, 66.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.149 CPU sec, 5.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12454, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (118.33, 65.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.44, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.270 CPU sec, 4.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12272, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 116, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (174.56, 142.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.47, 65.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.639 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12141, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 163, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (156.38, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.65, 65.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.394 CPU sec, 4.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12244, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 168, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (130.45, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.55, 65.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.101 CPU sec, 6.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12300, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 103, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (107.83, 47.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.59, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.982 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12375, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 90, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 200) [M::mem_pestat] mean and std.dev: (99.47, 36.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.72, 66.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.963 CPU sec, 5.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12501, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 111, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (109.46, 64.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.62, 68.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.526 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12389, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (116.88, 77.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (160.77, 68.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.557 CPU sec, 4.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12540, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (129.23, 66.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.12, 67.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.194 CPU sec, 3.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12460, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 75, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (108.83, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.00, 68.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.930 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12381, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (118.82, 59.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (160.49, 67.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.636 CPU sec, 4.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12627, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (108.26, 59.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.01, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.354 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12439, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (112.06, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.71, 67.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.404 CPU sec, 4.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12260, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 74, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (106.00, 59.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.91, 66.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.893 CPU sec, 4.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12357, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 97, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (102.18, 47.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.11, 66.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.155 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12331, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (124.63, 61.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.45, 65.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.700 CPU sec, 5.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12351, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 84, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (102.50, 76.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.03, 65.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.947 CPU sec, 4.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12230, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (131.10, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.65, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.279 CPU sec, 5.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12189, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 130, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (129.33, 61.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (158.04, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.004 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12343, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (116.44, 56.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (159.15, 66.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.916 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12318, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (112.80, 36.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (156.87, 64.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.510 CPU sec, 4.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12323, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 108, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (114.67, 57.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.18, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.174 CPU sec, 4.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12389, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 74, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (91.44, 42.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (156.42, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.005 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12348, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 147, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (161.10, 68.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.76, 66.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.885 CPU sec, 3.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12343, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 141, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (132.29, 78.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.88, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.744 CPU sec, 4.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12286, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.33, 66.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.112 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12428, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (113.80, 63.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.68, 67.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.415 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12454, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 127, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (139.94, 62.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.82, 66.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.754 CPU sec, 4.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12285, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 111, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (153.63, 100.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.95, 66.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.178 CPU sec, 4.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12285, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 129, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (129.78, 70.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.25, 64.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.873 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12233, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 87, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (99.10, 52.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.03, 64.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.301 CPU sec, 4.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12364, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 82, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (90.25, 51.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (157.96, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.834 CPU sec, 4.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12303, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 104, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (139.86, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (159.99, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.824 CPU sec, 4.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12320, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 118, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (117.06, 33.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.58, 70.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.424 CPU sec, 5.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12355, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 85, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (97.14, 49.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.27, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.484 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12060, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 142, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (158.94, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.20, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.155 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12405, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (122.42, 65.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (162.06, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.831 CPU sec, 4.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12321, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 83, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (147.82, 111.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.15, 68.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.857 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12332, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 108, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (128.91, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.13, 66.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.592 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12392, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 119, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (134.06, 83.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.20, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.605 CPU sec, 4.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12394, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (133.16, 83.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.60, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.365 CPU sec, 4.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12485, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (167.35, 111.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.70, 66.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.408 CPU sec, 5.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12386, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 114, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (99.11, 29.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.85, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.812 CPU sec, 5.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12560, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 84, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (105.58, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.60, 66.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.546 CPU sec, 4.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12597, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (114.62, 62.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.32, 66.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.355 CPU sec, 4.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12197, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (146.10, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.61, 64.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.050 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12105, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 106, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (114.06, 42.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.28, 65.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.119 CPU sec, 4.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12060, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (135.55, 63.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.29, 66.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.842 CPU sec, 4.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12338, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (137.33, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.01, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.294 CPU sec, 4.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12354, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 175, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (172.53, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (163.12, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.322 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12311, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 106, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (123.78, 76.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (163.64, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.856 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12250, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 95, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (93.82, 39.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (162.71, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.038 CPU sec, 5.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12256, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 98, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (91.06, 41.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.11, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.075 CPU sec, 4.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12291, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 93, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (112.40, 65.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.16, 69.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.740 CPU sec, 4.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12374, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 78, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (91.28, 60.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (161.42, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.416 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12503, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 113, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (136.61, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (161.93, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.769 CPU sec, 5.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12223, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.54, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (160.87, 67.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.590 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12424, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (124.19, 46.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (161.30, 67.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.857 CPU sec, 4.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12297, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 80, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (106.72, 81.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.45, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.090 CPU sec, 4.631 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12465, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 117, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 249) [M::mem_pestat] mean and std.dev: (117.25, 50.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.73, 64.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.891 CPU sec, 4.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12207, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 83, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (106.91, 62.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.71, 65.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.737 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11890, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 86, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (137.50, 136.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (158.01, 65.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.537 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11931, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 155, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (153.80, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (160.04, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.243 CPU sec, 4.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12219, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 109, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (129.29, 65.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.38, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.316 CPU sec, 5.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12175, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (116.21, 63.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.84, 70.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.796 CPU sec, 3.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12331, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (139.27, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (164.11, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.293 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12388, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 134, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (136.89, 79.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.21, 68.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.128 CPU sec, 5.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12358, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 123, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (131.50, 75.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.47, 68.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.358 CPU sec, 5.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12254, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (153.12, 81.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.03, 68.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.622 CPU sec, 4.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12328, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 163, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (239.31, 209.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (161.20, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.288 CPU sec, 5.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12424, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 165, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (134.12, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.36, 69.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.628 CPU sec, 4.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12448, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (121.00, 58.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.54, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.709 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12390, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (117.88, 55.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.89, 66.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.136 CPU sec, 5.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12240, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 102, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (102.72, 38.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.67, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.068 CPU sec, 5.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12235, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 95, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (120.20, 69.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.56, 66.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.419 CPU sec, 4.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12289, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 186, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (153.75, 74.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (156.28, 63.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.724 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11978, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 107, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (130.30, 81.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.08, 64.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.407 CPU sec, 5.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11895, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 135, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (122.79, 49.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.23, 66.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.330 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12290, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (111.62, 43.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.98, 68.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.755 CPU sec, 5.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12369, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 108, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (124.69, 77.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (160.12, 67.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.219 CPU sec, 4.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12228, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 71, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (99.72, 54.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.55, 66.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.824 CPU sec, 5.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12209, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 88, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (110.47, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.39, 66.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.300 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12436, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 98, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (102.29, 52.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.32, 66.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.450 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12098, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 174, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (137.90, 67.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.73, 68.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.279 CPU sec, 4.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12536, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 82, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (105.69, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.59, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.620 CPU sec, 4.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12449, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 54, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (74.33, 48.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.92, 66.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.999 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12472, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (114.00, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.86, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.527 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12359, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 96, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (136.53, 80.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.83, 66.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.035 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12480, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 97, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (106.38, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.31, 65.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.494 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12439, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (126.62, 62.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.75, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.250 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12220, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 95, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (111.73, 61.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (157.99, 65.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.601 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12042, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 99, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (121.17, 59.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (157.26, 63.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.040 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12167, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 96, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (104.27, 49.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.91, 63.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.685 CPU sec, 6.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12421, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 133, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (120.06, 43.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.74, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.542 CPU sec, 4.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12503, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 64, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (71.18, 32.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (162.36, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.532 CPU sec, 4.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12220, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 77, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (138.86, 127.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.80, 69.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.637 CPU sec, 4.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12315, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 93, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (104.67, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.31, 67.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.599 CPU sec, 4.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12278, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 57, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (117.69, 103.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.68, 66.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.263 CPU sec, 4.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12331, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (140.90, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.94, 68.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.770 CPU sec, 4.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12434, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 117, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (123.67, 65.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.81, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.145 CPU sec, 5.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12360, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 101, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (110.83, 57.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (159.95, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.804 CPU sec, 6.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12262, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 129, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (139.79, 81.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.71, 67.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.999 CPU sec, 5.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12298, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 94, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (109.81, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.45, 65.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.961 CPU sec, 5.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12531, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.80, 64.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.058 CPU sec, 4.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12424, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (23, 115, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (122.90, 93.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.48, 63.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.957 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12296, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 91, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (111.21, 65.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (158.07, 64.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.482 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12010, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (134.44, 91.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (155.53, 62.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.212 CPU sec, 5.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11951, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 180, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (163.90, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (158.22, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.134 CPU sec, 5.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12465, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (112.00, 51.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (159.91, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.740 CPU sec, 4.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12356, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 134, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (127.05, 56.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.46, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.743 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12176, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 83, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (105.24, 51.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.05, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.159 CPU sec, 5.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12279, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (129.56, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (159.62, 67.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.217 CPU sec, 6.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12423, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 123, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (117.58, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.34, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.597 CPU sec, 4.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12377, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (135.19, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.27, 68.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.694 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12387, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (95.31, 45.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.15, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.769 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12363, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 149, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (159.57, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (161.35, 68.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.183 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12311, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.01, 66.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.954 CPU sec, 6.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12346, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (170.24, 141.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.38, 66.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.960 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12445, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 100, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (109.36, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (156.79, 64.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.958 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12293, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 96, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (96.82, 33.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.45, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.829 CPU sec, 4.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12148, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 123, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (125.33, 53.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (157.02, 63.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.610 CPU sec, 4.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11938, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 95, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (109.64, 62.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (157.29, 64.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.961 CPU sec, 4.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12121, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 101, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (109.08, 51.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.53, 66.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.152 CPU sec, 4.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12281, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 100, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (125.00, 76.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.06, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.846 CPU sec, 4.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12450, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 197, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (206.36, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.68, 67.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.851 CPU sec, 5.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12200, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 163, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (138.79, 55.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.32, 66.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.906 CPU sec, 5.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12414, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 93, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (114.76, 61.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (159.12, 65.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.994 CPU sec, 4.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12351, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 140, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (134.75, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.25, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.405 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12331, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 124, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (179.50, 122.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.27, 68.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.219 CPU sec, 5.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12421, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 72, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (77.63, 43.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.36, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.900 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12524, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 88, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (111.38, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.14, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.796 CPU sec, 4.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12207, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 92, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (121.96, 83.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.25, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.918 CPU sec, 4.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12064, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 123, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (137.06, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.72, 68.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.805 CPU sec, 4.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12099, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.58, 65.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 113.676 CPU sec, 4.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12316, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 103, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (112.44, 61.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.34, 66.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.330 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12183, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 85, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (109.60, 61.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.38, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.824 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12331, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 91, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (147.58, 110.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.42, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.517 CPU sec, 5.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12487, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 96, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (132.75, 100.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (159.92, 66.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.236 CPU sec, 4.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12448, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 91, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (75.29, 35.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.17, 65.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.087 CPU sec, 4.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12357, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 86, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (111.28, 81.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.03, 66.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.944 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12312, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 90, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (104.06, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.12, 67.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.192 CPU sec, 4.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12332, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 128, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (125.60, 60.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (160.04, 66.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.123 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12337, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (123.18, 59.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.76, 69.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.725 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12371, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 103, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (116.50, 74.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.06, 66.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.753 CPU sec, 4.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12342, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (112.35, 80.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.84, 66.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.028 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12327, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 137, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (188.92, 119.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.72, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.281 CPU sec, 5.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12150, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (114.40, 55.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.22, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.723 CPU sec, 4.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12133, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 87, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (84.20, 54.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (159.93, 66.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.407 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12175, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 107, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (114.56, 59.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.27, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.853 CPU sec, 4.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12377, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 107, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (114.71, 53.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.85, 66.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.158 CPU sec, 4.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12336, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 98, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (117.21, 55.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.48, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.519 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12369, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 203, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 325) [M::mem_pestat] mean and std.dev: (185.67, 60.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.80, 68.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.865 CPU sec, 4.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12493, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 95, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (125.61, 72.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (158.86, 65.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.843 CPU sec, 4.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12484, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 89, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (111.53, 85.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.84, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.151 CPU sec, 4.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12503, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 67, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (95.17, 68.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.83, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.707 CPU sec, 3.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12403, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 68, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (75.50, 38.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.75, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.631 CPU sec, 5.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12376, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 129, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (126.60, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.45, 69.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.176 CPU sec, 5.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12223, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 89, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (111.71, 61.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.10, 68.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.995 CPU sec, 5.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12419, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 104, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (153.10, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (161.32, 68.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.732 CPU sec, 4.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12388, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (110.06, 49.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.48, 67.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.664 CPU sec, 4.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12396, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 78, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (87.00, 34.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.34, 65.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.897 CPU sec, 5.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12079, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 133, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (118.11, 52.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (159.22, 65.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.455 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12136, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 127, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (165.45, 139.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.00, 66.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.940 CPU sec, 4.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12346, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 102, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (147.83, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.12, 67.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.096 CPU sec, 6.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12464, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (147.11, 100.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.17, 66.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.111 CPU sec, 4.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12331, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (145.12, 140.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.15, 68.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.802 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12380, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 99, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (102.50, 44.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.89, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.900 CPU sec, 4.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12362, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 94, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (109.73, 86.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.23, 66.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.360 CPU sec, 5.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12334, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (113.50, 63.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.60, 66.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.413 CPU sec, 4.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12373, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (108.50, 56.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.34, 66.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.276 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12310, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 137, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (125.31, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.22, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.879 CPU sec, 4.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12373, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 156, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (176.41, 143.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.75, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.017 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12464, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 79, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (111.10, 81.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.32, 67.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.403 CPU sec, 6.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12487, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 104, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (105.29, 45.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (160.02, 67.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.210 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12328, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.32, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.969 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12300, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 127, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (124.12, 39.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.59, 65.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.885 CPU sec, 4.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12170, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 152, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (134.17, 57.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.49, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.557 CPU sec, 5.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12335, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 81, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (102.73, 71.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.69, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.514 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12441, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.81, 66.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.046 CPU sec, 6.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12250, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 120, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 190) [M::mem_pestat] mean and std.dev: (114.17, 28.12) [M::mem_pestat] low and high boundaries for proper pairs: (2, 227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.95, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.250 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12462, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (109.62, 51.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.78, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.597 CPU sec, 5.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12434, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (152.14, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.62, 65.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.291 CPU sec, 4.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12397, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 135, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (159.67, 113.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.65, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.377 CPU sec, 5.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12317, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 138, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (158.65, 88.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.59, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.536 CPU sec, 4.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12372, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (126.00, 85.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.49, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.443 CPU sec, 4.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12402, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (159.86, 100.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.49, 68.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.183 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12360, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 132, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (133.36, 67.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.40, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.832 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12300, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 94, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (117.18, 66.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (159.85, 65.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.770 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12437, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 127, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (145.47, 64.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.08, 67.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.539 CPU sec, 4.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12529, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 108, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (113.76, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.28, 66.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.025 CPU sec, 4.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12091, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 97, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.06, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.52, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.575 CPU sec, 4.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12231, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (110.79, 54.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.15, 65.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.834 CPU sec, 4.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12320, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (123.00, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.77, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.487 CPU sec, 4.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12443, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 134, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (135.58, 78.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (160.16, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.273 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12452, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 124, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (127.95, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.73, 66.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.569 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12553, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 87, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (107.54, 49.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.36, 65.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.352 CPU sec, 5.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12450, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 128, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (152.00, 109.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (158.00, 65.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.119 CPU sec, 6.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12515, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 105, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (111.00, 55.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.49, 65.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.987 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12422, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 71, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (68.00, 28.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.92, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.035 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12457, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (104.86, 43.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.04, 66.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.099 CPU sec, 4.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12402, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 70, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (83.71, 28.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.32, 65.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.950 CPU sec, 5.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12334, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (110.31, 68.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.83, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.877 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12385, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 117, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (154.89, 114.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.99, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.049 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12285, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 129, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (135.25, 69.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (159.01, 66.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.980 CPU sec, 5.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12363, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 140, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (141.15, 63.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.04, 65.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.614 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12170, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 110, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (130.28, 72.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (158.11, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.612 CPU sec, 5.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12278, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (150.29, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.46, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.092 CPU sec, 4.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12257, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 136, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (143.94, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.28, 64.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.296 CPU sec, 5.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12262, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (127.88, 55.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.51, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.215 CPU sec, 3.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12407, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 110, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (140.23, 104.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.63, 67.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.949 CPU sec, 4.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12337, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (130.43, 86.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.18, 67.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.686 CPU sec, 4.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12234, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.45, 64.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 103.807 CPU sec, 3.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12317, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 97, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (100.83, 65.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (157.89, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.105 CPU sec, 3.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11950, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 112, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (144.75, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (158.15, 64.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.006 CPU sec, 4.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11989, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 124, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 237) [M::mem_pestat] mean and std.dev: (115.62, 49.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (158.15, 65.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.834 CPU sec, 4.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12393, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 122, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (139.69, 77.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.61, 66.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.217 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12400, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 110, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (128.50, 63.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.75, 67.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.382 CPU sec, 5.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12346, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 176, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (156.47, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (161.20, 68.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.595 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12451, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 102, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (133.33, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.17, 67.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.254 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12271, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 99, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (95.58, 35.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.82, 65.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.858 CPU sec, 5.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12302, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 90, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (96.60, 58.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.81, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.831 CPU sec, 5.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12245, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (136.58, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.71, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.138 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12368, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.68, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.295 CPU sec, 4.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12383, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 120, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (142.17, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.12, 66.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.552 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12410, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 117, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 240) [M::mem_pestat] mean and std.dev: (120.67, 43.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.65, 67.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.787 CPU sec, 4.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12393, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 105, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (126.89, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.67, 65.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.656 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12304, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 97, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (126.87, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (157.85, 64.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.240 CPU sec, 5.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12106, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 190, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (168.14, 77.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (156.73, 63.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.904 CPU sec, 4.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11819, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 106, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (98.67, 22.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.63, 65.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.866 CPU sec, 4.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12272, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 100, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (94.40, 40.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.60, 68.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.832 CPU sec, 4.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12438, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (119.60, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.58, 67.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.629 CPU sec, 3.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12340, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 124, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (126.15, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.40, 67.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.858 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12454, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 86, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (116.23, 87.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.70, 68.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.011 CPU sec, 5.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12475, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 92, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (101.05, 62.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.52, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.462 CPU sec, 4.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12356, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (138.14, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.75, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.730 CPU sec, 5.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12375, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 152, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (142.25, 59.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (162.72, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.476 CPU sec, 4.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12283, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 75, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (69.71, 34.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (163.43, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.810 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12440, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 85, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (106.18, 64.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (163.59, 70.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.469 CPU sec, 4.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12337, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 102, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (117.92, 67.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (162.87, 69.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.513 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12387, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 103, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (100.30, 47.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.47, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.550 CPU sec, 4.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12201, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 93, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (94.70, 56.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.73, 65.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.217 CPU sec, 4.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12207, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 142, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (133.38, 80.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.94, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.934 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12261, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (116.62, 58.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (155.13, 62.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.190 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12103, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 127, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (119.88, 46.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.16, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.404 CPU sec, 3.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12480, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 123, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (98.89, 51.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.79, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.002 CPU sec, 4.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12406, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 100, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (148.77, 107.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.16, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.915 CPU sec, 3.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12419, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 103, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (100.79, 54.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.42, 67.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.762 CPU sec, 4.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12556, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (148.81, 116.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.60, 67.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.732 CPU sec, 3.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12576, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 159, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (126.82, 55.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.79, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.857 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12393, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (103.92, 50.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.98, 68.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.576 CPU sec, 3.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12402, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 75, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 177) [M::mem_pestat] mean and std.dev: (82.21, 29.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (161.78, 69.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.711 CPU sec, 4.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12362, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 97, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (109.71, 54.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.85, 69.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.035 CPU sec, 4.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12336, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (90.27, 44.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.83, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.654 CPU sec, 3.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12449, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 122, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (138.92, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (161.07, 68.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.939 CPU sec, 4.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12450, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 84, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (87.94, 45.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.89, 64.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.791 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12355, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 109, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (106.47, 58.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (155.93, 63.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.704 CPU sec, 4.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12129, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 133, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (118.38, 52.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (156.68, 62.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.462 CPU sec, 3.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11809, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 90, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 185) [M::mem_pestat] mean and std.dev: (86.89, 20.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 143, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (154.45, 62.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.513 CPU sec, 4.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12182, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (134.77, 61.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (158.13, 65.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.716 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12262, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (131.14, 74.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.77, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.649 CPU sec, 4.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12407, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (137.08, 100.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.82, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.508 CPU sec, 3.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12269, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 104, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (86.71, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.80, 66.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.222 CPU sec, 3.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12341, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 123, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (109.68, 44.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (160.17, 67.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.154 CPU sec, 4.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12302, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 90, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (125.27, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.03, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.314 CPU sec, 4.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12261, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 95, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (132.90, 106.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.24, 66.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.721 CPU sec, 4.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12364, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 119, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (131.85, 85.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.20, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.984 CPU sec, 4.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12313, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 83, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (115.64, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.66, 68.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.910 CPU sec, 3.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12355, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 98, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.20, 77.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.36, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.518 CPU sec, 3.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12438, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (139.33, 82.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.17, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.016 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12287, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (111.27, 86.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (155.46, 63.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.714 CPU sec, 4.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12375, 4, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (157.36, 64.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 100.168 CPU sec, 3.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12007, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 98, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (131.47, 92.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (155.11, 62.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.717 CPU sec, 4.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11834, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 101, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (152.81, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (155.82, 63.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.642 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12222, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (108.12, 64.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.45, 65.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.071 CPU sec, 4.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12347, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (118.85, 73.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (157.03, 64.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.866 CPU sec, 4.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12356, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (120.90, 60.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.04, 64.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.076 CPU sec, 3.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12552, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 120, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (147.17, 84.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (158.37, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.791 CPU sec, 3.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12370, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 102, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (91.76, 44.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.42, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.200 CPU sec, 4.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12463, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (130.22, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.58, 68.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.187 CPU sec, 4.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12485, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 160, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (148.20, 76.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.39, 66.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.043 CPU sec, 4.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12283, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 71, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (57.00, 28.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.92, 66.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.733 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12602, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (137.05, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.63, 70.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.911 CPU sec, 3.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12286, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 136, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (133.83, 85.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.11, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.317 CPU sec, 3.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12421, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 96, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (123.11, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.78, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.159 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12367, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 103, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (100.74, 42.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.82, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.225 CPU sec, 3.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12499, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (104.19, 49.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (155.76, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.730 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12323, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (119.25, 51.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (156.55, 64.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.056 CPU sec, 3.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12054, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (138.33, 67.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (159.26, 66.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.665 CPU sec, 5.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12334, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 79, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (81.07, 30.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.00, 67.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.499 CPU sec, 5.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12295, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 99, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (106.92, 57.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.35, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.135 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12396, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 102, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (134.62, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.82, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.099 CPU sec, 4.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12328, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 108, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (121.79, 64.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.35, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.464 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12380, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 83, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (89.76, 44.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.73, 67.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.100 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12361, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (176.62, 120.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.05, 68.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.007 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12368, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 154, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (141.11, 61.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (159.24, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.032 CPU sec, 5.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12385, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (160.02, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.566 CPU sec, 5.305 real sec [M::process] read 17444 sequences (2634044 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12319, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 86, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (108.60, 52.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.85, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.532 CPU sec, 4.357 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 3316, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (156.86, 64.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 17444 reads in 29.554 CPU sec, 1.502 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21164\tSM:UG10S2P154\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21164_HLKNYBGXX_UG10S2P154_R1.fastq.gz 5347_7332_21164_HLKNYBGXX_UG10S2P154_R2.fastq.gz [main] Real time: 1738.769 sec; CPU: 42212.567 sec Finished alignement of UG10S2P154 at Wed Apr 3 07:37:48 2019 Sorting BAM file started at Wed Apr 3 07:37:48 2019 samtools sort -@ 30 -m 4G UG10S2P154_unsorted.bam -o UG10S2P154.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 07:37:48 2019 rm UG10S2P154_unsorted.bam rm 5347_7332_21164_HLKNYBGXX_UG10S2P154_R1.fastq.gz rm 5347_7332_21164_HLKNYBGXX_UG10S2P154_R2.fastq.gz samtools index UG10S2P154.bam Finished BAM file generation of the sample - UG10S2P154 Processing fastq files for the sample - UG10S2P166 Reading 5347_7332_21165_HLKNYBGXX_UG10S2P166_R1.fastq.gz 5347_7332_21165_HLKNYBGXX_UG10S2P166_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21165\tSM:UG10S2P166\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21165_HLKNYBGXX_UG10S2P166_R1.fastq.gz 5347_7332_21165_HLKNYBGXX_UG10S2P166_R2.fastq.gz | samtools view -Sb1 - > UG10S2P166_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12799, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 191, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (173.80, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (206.46, 105.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.433 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13030, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 95, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (124.74, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.99, 104.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.480 CPU sec, 4.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13034, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (153.93, 106.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.66, 106.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.623 CPU sec, 4.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13099, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 140, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (177.19, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.20, 107.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.148 CPU sec, 6.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13104, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (162.53, 83.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (210.24, 107.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.732 CPU sec, 5.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13160, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 137, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (126.95, 84.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.47, 108.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.267 CPU sec, 4.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13138, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 83, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (168.90, 170.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.40, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.532 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13174, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (128.56, 83.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (210.05, 109.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.668 CPU sec, 6.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13354, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (152.57, 111.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (202.87, 101.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.235 CPU sec, 5.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13193, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 136, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (139.25, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.58, 102.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.936 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13233, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (134.94, 74.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (205.05, 102.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.423 CPU sec, 6.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13130, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (157.94, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.93, 101.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.710 CPU sec, 5.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12947, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 166, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (177.78, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (203.22, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.261 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12813, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 187, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (197.92, 144.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (206.92, 104.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.869 CPU sec, 4.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12923, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 156, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (136.38, 56.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.21, 104.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.450 CPU sec, 6.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12867, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 151, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (145.36, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (207.57, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.634 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12984, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (156.50, 57.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (209.01, 106.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.175 CPU sec, 6.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13198, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (124.74, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (209.73, 109.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.822 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13115, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 158, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (183.00, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (205.97, 105.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.745 CPU sec, 5.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13164, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 167, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (150.29, 80.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (209.27, 108.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.132 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13204, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 155, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (153.25, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.24, 104.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.788 CPU sec, 4.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13264, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 163, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (177.35, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (206.01, 103.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.672 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13207, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 133, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (164.95, 113.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.97, 107.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.706 CPU sec, 6.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13102, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 136, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (157.08, 109.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.87, 102.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.645 CPU sec, 6.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13111, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (191.27, 139.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.52, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.320 CPU sec, 4.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13020, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 139, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (147.00, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.79, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.830 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12886, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.82, 99.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.026 CPU sec, 5.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12943, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (114.13, 59.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (208.07, 105.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.700 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13110, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (136.00, 62.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.66, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.816 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13058, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 121, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (182.35, 125.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.56, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.685 CPU sec, 6.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12958, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 89, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (105.82, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (208.59, 106.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.553 CPU sec, 4.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13103, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 186, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (204.53, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.06, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.224 CPU sec, 6.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12978, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (162.36, 70.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.21, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.024 CPU sec, 5.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13209, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 92, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (109.55, 68.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (206.01, 104.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.407 CPU sec, 5.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13013, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (170.76, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.46, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.154 CPU sec, 5.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13053, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (153.06, 71.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.61, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.064 CPU sec, 5.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12978, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 160, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (182.25, 115.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.82, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.676 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13094, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (126.00, 45.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.67, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.429 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12876, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 117, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (132.57, 76.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.26, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.966 CPU sec, 6.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12926, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 109, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (136.19, 103.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (199.43, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.130 CPU sec, 5.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12764, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 138, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (126.46, 61.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (202.05, 100.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.379 CPU sec, 6.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13016, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 95, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (108.94, 61.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (203.26, 102.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.076 CPU sec, 6.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13035, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 103, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (123.75, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (206.24, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.824 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13210, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.24, 107.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.888 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13067, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (123.33, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.46, 102.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.818 CPU sec, 5.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13118, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.73, 105.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.274 CPU sec, 5.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13295, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 105, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (148.53, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (206.58, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.631 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13260, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 108, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (133.69, 88.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (205.99, 103.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.304 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12982, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 119, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (109.62, 60.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.93, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.881 CPU sec, 4.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12997, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (149.95, 100.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (201.73, 100.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.781 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13128, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (147.25, 76.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (204.09, 102.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.762 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13064, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (107.68, 60.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.97, 100.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.008 CPU sec, 5.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13074, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (151.32, 99.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.45, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.815 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12597, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 92, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (121.29, 85.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.99, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.989 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13023, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 212, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (160.00, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.94, 99.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.250 CPU sec, 5.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13139, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (110.89, 45.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.66, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.933 CPU sec, 4.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13071, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 113, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (93.00, 34.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.29, 103.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.614 CPU sec, 5.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13175, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 149, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (224.14, 226.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.69, 104.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.771 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12964, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 170, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (201.00, 158.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.57, 100.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.936 CPU sec, 5.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13086, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 117, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (162.30, 110.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (206.20, 104.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.906 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13201, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 118, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (120.22, 67.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.91, 103.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.916 CPU sec, 4.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13048, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 157, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 344) [M::mem_pestat] mean and std.dev: (160.82, 54.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.61, 101.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.659 CPU sec, 6.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13001, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 147, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (140.47, 94.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.52, 103.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.534 CPU sec, 5.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13019, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 154, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (184.94, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (202.71, 100.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.690 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13058, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 194, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (187.31, 106.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.61, 101.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.393 CPU sec, 5.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13050, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 109, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (122.77, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.18, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.086 CPU sec, 5.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12988, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 88, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (120.40, 81.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.44, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.566 CPU sec, 5.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12703, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (161.07, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.34, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.720 CPU sec, 5.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13042, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (120.56, 75.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.41, 102.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.562 CPU sec, 5.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13096, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 147, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (160.56, 92.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.31, 105.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.332 CPU sec, 5.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13039, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 114, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (136.23, 61.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (201.03, 99.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.098 CPU sec, 6.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12997, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 137, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (148.74, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.09, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.354 CPU sec, 4.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13056, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 78, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (122.45, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.37, 102.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.918 CPU sec, 5.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12998, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 161, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (183.67, 139.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (205.23, 103.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.325 CPU sec, 5.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12925, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 127, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (124.37, 66.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (203.00, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.076 CPU sec, 5.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12961, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (139.65, 81.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (203.03, 101.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.193 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13232, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 172, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (165.16, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.15, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.151 CPU sec, 4.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12966, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (148.40, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (199.91, 98.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.671 CPU sec, 6.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13039, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 118, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (174.53, 142.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.64, 98.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.915 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12993, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 158, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (124.11, 52.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.06, 99.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.454 CPU sec, 5.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13014, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 161, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (154.23, 82.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.56, 105.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.951 CPU sec, 4.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13171, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 225, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (165.44, 110.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (209.33, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.531 CPU sec, 5.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13052, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 91, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (147.58, 135.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.81, 106.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.149 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13177, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 163, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (146.65, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (207.13, 105.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.063 CPU sec, 5.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12965, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 135, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (160.67, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (208.95, 107.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.295 CPU sec, 4.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13241, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 98, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (140.69, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.21, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.519 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13046, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 166, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (161.27, 68.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.31, 104.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.418 CPU sec, 5.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13234, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 131, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (122.73, 45.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (202.68, 102.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.224 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13057, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (157.40, 95.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (204.69, 103.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.899 CPU sec, 5.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13086, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 89, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (130.33, 114.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (199.51, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.009 CPU sec, 6.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12791, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (131.69, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.35, 98.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.092 CPU sec, 4.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12484, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 170, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (160.46, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (195.84, 94.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.131 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12516, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (172.14, 150.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.76, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.609 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13142, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 173, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (208.52, 142.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (211.98, 109.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.931 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13031, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 129, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (177.12, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (214.22, 111.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.263 CPU sec, 6.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13056, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 120, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (177.80, 100.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 177, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (211.17, 108.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.948 CPU sec, 5.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12951, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 81, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (91.57, 47.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (211.13, 108.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.438 CPU sec, 5.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13234, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (147.25, 117.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (207.80, 104.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.283 CPU sec, 5.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13342, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 135, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (145.85, 58.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (212.71, 110.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.114 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13004, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 153, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (164.75, 91.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.50, 105.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.789 CPU sec, 5.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12909, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 157, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (155.71, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.83, 107.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.406 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13148, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 87, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (96.50, 53.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (207.37, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.763 CPU sec, 6.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12959, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 130, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (139.79, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.37, 100.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.126 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12949, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 82, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (116.00, 85.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (195.86, 94.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.956 CPU sec, 5.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12595, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 107, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (143.30, 112.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.17, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.658 CPU sec, 6.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12448, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 184, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (191.84, 108.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.85, 95.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.599 CPU sec, 7.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12976, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (164.00, 100.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.47, 107.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.660 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13201, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 147, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (136.58, 87.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (210.73, 109.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.137 CPU sec, 5.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13161, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (140.59, 94.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.67, 106.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.214 CPU sec, 4.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13062, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (137.46, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (209.03, 107.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.887 CPU sec, 4.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13140, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 165, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474) [M::mem_pestat] mean and std.dev: (391.45, 487.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 178, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (212.25, 109.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.712 CPU sec, 4.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13074, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 203, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (187.09, 83.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (209.63, 107.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.600 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13054, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 113, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (123.12, 56.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (208.76, 106.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.700 CPU sec, 6.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13050, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 110, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (140.78, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (208.75, 106.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.789 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13212, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 112, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (148.18, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (203.60, 102.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.435 CPU sec, 4.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13018, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 140, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (132.32, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.84, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.200 CPU sec, 5.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12904, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (155.15, 103.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (199.38, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.842 CPU sec, 5.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12941, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 140, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (105.00, 55.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (199.13, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.169 CPU sec, 4.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12401, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 141, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (140.92, 80.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.75, 94.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.056 CPU sec, 6.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12830, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 149, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (170.26, 103.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.82, 102.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.910 CPU sec, 6.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12949, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 168, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (150.00, 91.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (205.85, 103.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.810 CPU sec, 5.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13018, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 79, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (109.42, 75.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (205.76, 103.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.351 CPU sec, 5.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13123, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (112.33, 56.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.40, 102.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.065 CPU sec, 4.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12864, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 99, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (121.80, 79.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (206.92, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.567 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13065, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 238, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (192.82, 112.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (206.16, 104.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.772 CPU sec, 5.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12911, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 120, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (105.57, 66.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (206.68, 104.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.640 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13055, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.30, 101.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.586 CPU sec, 5.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13142, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (144.38, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (201.80, 100.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.293 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13213, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 107, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (110.11, 47.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (200.21, 99.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.587 CPU sec, 4.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13073, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 79, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (78.56, 27.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (199.13, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.033 CPU sec, 4.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12790, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 143, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (148.00, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.62, 93.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.125 CPU sec, 5.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12647, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.71, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.646 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12658, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 151, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (181.36, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.25, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.911 CPU sec, 5.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13136, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.24, 105.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.677 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13097, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (130.13, 85.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.68, 104.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.028 CPU sec, 4.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12974, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (138.60, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (208.33, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.223 CPU sec, 5.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13039, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (206.01, 104.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.884 CPU sec, 6.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13033, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (110.53, 59.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (206.67, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.621 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13298, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 117, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (134.78, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (206.15, 103.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.545 CPU sec, 6.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13183, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 126, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (124.83, 56.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.62, 105.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.738 CPU sec, 4.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13232, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (118.64, 64.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.13, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.679 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12916, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 102, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (90.83, 44.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (199.38, 98.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.010 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13048, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 85, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (85.11, 44.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (198.39, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.758 CPU sec, 6.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12929, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 138, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (148.84, 63.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.76, 96.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.219 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12559, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 144, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (138.36, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.16, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.717 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12503, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 106, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (122.12, 62.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.98, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.310 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12841, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 124, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (135.35, 73.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.61, 108.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.602 CPU sec, 5.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13106, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 117, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (120.67, 72.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.82, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.498 CPU sec, 5.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12959, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 130, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (126.77, 63.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.62, 104.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.202 CPU sec, 5.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13111, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 175, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (162.00, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.42, 105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.611 CPU sec, 4.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13204, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 149, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (147.00, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (207.37, 105.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.155 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12835, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 141, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (125.31, 52.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (208.37, 106.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.174 CPU sec, 5.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13219, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 95, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (105.93, 38.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.49, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.809 CPU sec, 6.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13079, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 140, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (155.90, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (204.28, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.222 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12925, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 118, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (117.83, 55.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (202.74, 101.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.454 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12878, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 134, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (155.29, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (198.84, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.766 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12975, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (180.72, 106.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.85, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.835 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12524, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 108, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (137.25, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.29, 95.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.249 CPU sec, 5.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12622, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (115.92, 36.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.92, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.962 CPU sec, 5.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13088, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 136, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (150.16, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.27, 100.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.902 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12984, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 100, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (149.24, 108.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.22, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.513 CPU sec, 4.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12913, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 107, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (127.36, 77.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.00, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.120 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13114, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 112, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (118.78, 44.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (204.51, 101.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.448 CPU sec, 5.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13133, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 124, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (162.48, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (206.52, 104.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.368 CPU sec, 5.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13142, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 178, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (180.45, 109.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.53, 103.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.617 CPU sec, 4.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13167, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 141, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (185.00, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (205.04, 103.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.298 CPU sec, 5.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13080, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 129, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (174.27, 104.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (206.87, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.174 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12934, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 105, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (119.88, 62.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.03, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.786 CPU sec, 4.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12888, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (150.91, 96.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.96, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.786 CPU sec, 6.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13031, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 191, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (201.09, 108.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.10, 100.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.678 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12963, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 130, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (118.17, 56.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (207.13, 106.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.616 CPU sec, 6.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13091, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (113.68, 53.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.60, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.548 CPU sec, 4.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13027, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 185, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (153.36, 112.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.77, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.761 CPU sec, 5.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12974, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (154.36, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (200.53, 99.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.966 CPU sec, 5.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13154, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (133.80, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (200.74, 98.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.493 CPU sec, 4.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12988, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 160, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (183.50, 114.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (203.53, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.002 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13062, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 159, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (174.32, 122.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (207.29, 104.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.913 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13203, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 168, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (176.47, 112.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (207.76, 105.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.083 CPU sec, 5.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13210, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 163, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (200.79, 119.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (206.82, 105.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.159 CPU sec, 4.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12910, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (203.73, 102.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.047 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12638, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 142, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (124.50, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (206.35, 104.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.282 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12886, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 247, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (197.56, 73.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.29, 99.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.678 CPU sec, 4.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12909, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 105, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (128.83, 85.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.74, 102.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.092 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13100, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 140, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (146.07, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.88, 105.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.679 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13111, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 153, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (158.50, 81.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (205.14, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.808 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12956, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (170.38, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.86, 99.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.203 CPU sec, 5.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13119, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 86, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (140.73, 104.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (198.22, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.806 CPU sec, 5.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12929, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 83, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (104.12, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (198.93, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.552 CPU sec, 6.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13012, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (179.61, 110.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.27, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.048 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13107, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (197.94, 179.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (208.69, 106.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.310 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13026, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 145, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (142.07, 59.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.51, 104.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.696 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13161, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 108, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (164.80, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (203.92, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.514 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13114, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 196, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (179.43, 109.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.10, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.951 CPU sec, 4.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12898, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 115, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (144.31, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (204.60, 101.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.026 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12873, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 101, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (130.69, 102.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (205.78, 103.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.519 CPU sec, 4.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13065, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 108, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (149.69, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (208.42, 107.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.493 CPU sec, 4.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12957, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (135.96, 77.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (208.31, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.149 CPU sec, 5.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13003, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 76, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (83.06, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.68, 102.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.210 CPU sec, 6.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13144, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (139.04, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.73, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.600 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13043, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 104, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (120.18, 83.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (201.48, 100.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.708 CPU sec, 4.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12953, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 142, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (150.64, 52.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.93, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.071 CPU sec, 5.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12886, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 133, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (110.67, 63.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (203.10, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.909 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12906, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 126, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (141.48, 84.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (205.55, 104.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.548 CPU sec, 5.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13133, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (104.59, 54.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (205.07, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.652 CPU sec, 5.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13081, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 152, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (118.78, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (203.64, 102.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.999 CPU sec, 6.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12971, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (152.79, 96.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.41, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.878 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12814, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 171, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (139.44, 54.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (196.53, 96.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.513 CPU sec, 5.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12743, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 123, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (105.20, 85.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (200.21, 99.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.282 CPU sec, 5.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13057, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (145.85, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.00, 100.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.997 CPU sec, 4.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13261, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 123, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (193.10, 183.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.10, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.358 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13154, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 156, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (156.19, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (202.64, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.019 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12919, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 159, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (164.08, 93.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.64, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.392 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13107, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 151, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (165.14, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.54, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.523 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12922, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 99, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (127.21, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (203.02, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.615 CPU sec, 5.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13121, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 127, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (135.88, 81.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (205.75, 105.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.156 CPU sec, 5.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13210, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 130, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (128.07, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (204.18, 102.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.579 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13162, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 108, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (166.71, 108.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (201.73, 100.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.970 CPU sec, 5.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13066, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 100, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (87.93, 34.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (205.28, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.699 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12926, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 159, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (152.82, 77.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.45, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.453 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12898, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 184, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (179.36, 94.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (199.31, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.260 CPU sec, 4.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12983, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 134, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (165.69, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (200.40, 99.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.787 CPU sec, 5.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13094, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (169.06, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (201.28, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.574 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13062, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 194, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (146.00, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (203.05, 101.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.857 CPU sec, 4.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13160, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 120, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (144.05, 86.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.90, 101.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.579 CPU sec, 4.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12952, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 88, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (126.21, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.53, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.381 CPU sec, 5.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13099, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 137, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (152.38, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (198.51, 96.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.814 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13008, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 159, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (210.71, 196.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.32, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.350 CPU sec, 6.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13029, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 102, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (120.93, 67.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.44, 103.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.295 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13110, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 187, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (181.92, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (199.62, 98.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.626 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12940, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 185, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (166.86, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.36, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.032 CPU sec, 4.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13074, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 124, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (119.50, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.18, 98.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.882 CPU sec, 5.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13028, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 116, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (121.46, 60.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.07, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.498 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12768, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (197.83, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.537 CPU sec, 5.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12931, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (140.18, 77.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (199.14, 98.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.875 CPU sec, 4.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13099, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (152.08, 117.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.27, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.139 CPU sec, 4.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13172, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 109, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (101.15, 31.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (205.37, 102.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.742 CPU sec, 5.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12965, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 137, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (157.84, 89.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.93, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.464 CPU sec, 5.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12953, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 94, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (109.43, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.09, 95.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.971 CPU sec, 5.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12630, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 123, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (116.04, 48.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.76, 96.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.164 CPU sec, 6.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12368, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 116, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (152.58, 117.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.22, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.820 CPU sec, 5.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12923, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (226.41, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (203.80, 102.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.605 CPU sec, 4.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13004, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 93, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (123.63, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (207.24, 106.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.334 CPU sec, 5.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13148, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 206, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 397) [M::mem_pestat] mean and std.dev: (193.27, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.69, 100.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.386 CPU sec, 4.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13175, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 100, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (130.38, 86.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.10, 101.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.414 CPU sec, 5.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13030, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 190, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (170.11, 84.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.66, 103.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.439 CPU sec, 4.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13158, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 108, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (117.53, 49.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.02, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.529 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13018, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 151, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (163.22, 78.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (204.82, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.487 CPU sec, 4.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13204, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (122.50, 60.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (206.05, 103.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.991 CPU sec, 4.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13202, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 82, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (114.57, 76.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.79, 103.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.943 CPU sec, 5.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12954, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (117.77, 47.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.28, 99.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.025 CPU sec, 4.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12968, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (121.00, 70.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.39, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.331 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12693, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 98, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (104.43, 52.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.48, 94.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.290 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12411, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 121, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (168.67, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.55, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.318 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13201, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 138, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (134.50, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (208.66, 107.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.068 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13280, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 90, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (97.50, 65.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.48, 104.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.512 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13181, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 155, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (150.90, 84.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (204.98, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.652 CPU sec, 5.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13107, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 228, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (195.67, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (205.52, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.328 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13056, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (206.74, 104.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.815 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13256, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 139, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (161.22, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (206.24, 105.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.891 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12989, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (150.53, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (207.60, 105.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.276 CPU sec, 5.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12984, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 156, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (139.78, 57.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (207.22, 106.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.841 CPU sec, 5.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13079, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 147, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (158.17, 103.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (207.41, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.851 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12959, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 118, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (148.12, 118.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (200.34, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.828 CPU sec, 6.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12930, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 141, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (142.71, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (198.84, 97.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.220 CPU sec, 4.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12616, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 119, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (150.94, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (198.99, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.746 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12523, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.10, 96.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.365 CPU sec, 6.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13165, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 127, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (135.90, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (207.74, 105.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.283 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13327, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 132, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (164.71, 96.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.75, 104.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.305 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13072, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 121, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (129.18, 56.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.60, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.673 CPU sec, 5.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13166, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 121, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (139.61, 69.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (205.16, 104.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.654 CPU sec, 5.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12999, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 167, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (144.82, 78.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (206.50, 103.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.022 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12867, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 254, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (293.10, 259.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (207.31, 105.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.945 CPU sec, 6.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13183, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 130, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (151.94, 78.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (206.90, 105.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.894 CPU sec, 5.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13057, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 72, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (85.10, 44.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 176, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (208.63, 107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.296 CPU sec, 6.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13088, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 170, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (190.21, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.76, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.123 CPU sec, 5.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13061, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (148.57, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.80, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.582 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12872, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 190, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (193.75, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.10, 94.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.204 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12489, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 136, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (164.62, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.87, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.393 CPU sec, 5.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12678, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 138, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 307) [M::mem_pestat] mean and std.dev: (161.33, 60.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.66, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.073 CPU sec, 6.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13062, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.59, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.021 CPU sec, 5.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13147, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 96, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (94.50, 50.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (204.29, 103.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.044 CPU sec, 4.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12992, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (137.38, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.84, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.683 CPU sec, 6.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13067, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 135, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (135.87, 69.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.29, 99.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.086 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12934, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 157, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (176.47, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.38, 101.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.597 CPU sec, 5.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13100, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (139.47, 101.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.75, 101.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.990 CPU sec, 4.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13085, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 155, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (175.73, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (205.59, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.634 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12855, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 157, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (174.85, 96.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (203.91, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.161 CPU sec, 5.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13086, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 122, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (137.73, 78.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.08, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.484 CPU sec, 5.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12881, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (141.74, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (195.67, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.007 CPU sec, 6.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12739, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (191.51, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.441 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12399, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 225, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (236.00, 150.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (190.10, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.504 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12846, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 100, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (132.00, 91.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.78, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.030 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13070, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 141, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (144.74, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (205.55, 103.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.920 CPU sec, 5.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13052, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 107, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (118.50, 71.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.24, 102.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.486 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12881, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 115, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (140.38, 90.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.20, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.912 CPU sec, 5.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13211, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (176.56, 86.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (200.38, 99.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.024 CPU sec, 6.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13068, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 129, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (135.18, 60.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (204.20, 102.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.969 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13156, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 118, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (124.69, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (206.35, 103.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.281 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13164, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (115.73, 58.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (205.10, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.730 CPU sec, 4.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13012, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 138, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (150.18, 104.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (205.25, 103.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.091 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13199, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 164, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (163.85, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (199.50, 99.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.686 CPU sec, 6.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13046, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 93, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (120.80, 103.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.98, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.527 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12902, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (127.85, 68.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (196.54, 95.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.253 CPU sec, 4.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12689, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (180.08, 132.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (192.34, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.151 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12938, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 141, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (135.95, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (203.15, 101.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.602 CPU sec, 5.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13045, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 105, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (178.80, 146.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (204.83, 103.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.096 CPU sec, 4.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13152, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 100, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (100.25, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (201.33, 100.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.662 CPU sec, 4.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13176, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (125.31, 36.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (203.42, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.308 CPU sec, 4.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13105, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 140, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (135.00, 69.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (201.95, 100.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.919 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13146, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 144, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (131.43, 68.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (205.65, 103.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.307 CPU sec, 4.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12952, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 356) [M::mem_pestat] mean and std.dev: (135.89, 58.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (204.56, 101.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.357 CPU sec, 4.839 real sec [M::process] read 64844 sequences (9791444 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12985, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 126, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (146.18, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.43, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.106 CPU sec, 5.258 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13022, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 86, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (95.92, 50.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (205.20, 104.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 64844 reads in 125.662 CPU sec, 4.590 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21165\tSM:UG10S2P166\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21165_HLKNYBGXX_UG10S2P166_R1.fastq.gz 5347_7332_21165_HLKNYBGXX_UG10S2P166_R2.fastq.gz [main] Real time: 1696.335 sec; CPU: 41053.675 sec Finished alignement of UG10S2P166 at Wed Apr 3 08:08:32 2019 Sorting BAM file started at Wed Apr 3 08:08:32 2019 samtools sort -@ 30 -m 4G UG10S2P166_unsorted.bam -o UG10S2P166.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 08:08:32 2019 rm UG10S2P166_unsorted.bam rm 5347_7332_21165_HLKNYBGXX_UG10S2P166_R1.fastq.gz rm 5347_7332_21165_HLKNYBGXX_UG10S2P166_R2.fastq.gz samtools index UG10S2P166.bam Finished BAM file generation of the sample - UG10S2P166 Processing fastq files for the sample - UG10S2P174 Reading 5347_7332_21166_HLKNYBGXX_UG10S2P174_R1.fastq.gz 5347_7332_21166_HLKNYBGXX_UG10S2P174_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21166\tSM:UG10S2P174\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21166_HLKNYBGXX_UG10S2P174_R1.fastq.gz 5347_7332_21166_HLKNYBGXX_UG10S2P174_R2.fastq.gz | samtools view -Sb1 - > UG10S2P174_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12524, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 109, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (108.00, 50.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (210.18, 99.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.518 CPU sec, 5.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12742, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (125.38, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (208.91, 98.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.777 CPU sec, 4.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12721, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (140.20, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (212.68, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.852 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12756, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 97, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (120.81, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (211.09, 100.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.316 CPU sec, 4.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12751, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 143, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (163.00, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (210.13, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.688 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12988, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 125, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (133.59, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (210.63, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.492 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12732, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 126, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (170.54, 115.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (212.71, 101.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.546 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12864, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (151.79, 100.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (208.88, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.733 CPU sec, 4.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12958, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (143.47, 86.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.24, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.668 CPU sec, 4.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12651, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (133.83, 61.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (208.75, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.231 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12760, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 136, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (129.70, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (205.50, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.769 CPU sec, 5.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12684, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (121.53, 61.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.18, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.200 CPU sec, 4.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12621, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 136, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (146.38, 79.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (205.86, 95.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.372 CPU sec, 4.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12380, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 175, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (173.00, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.12, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.624 CPU sec, 6.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12549, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (148.00, 91.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.06, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.685 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12770, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 140, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (170.88, 101.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (209.25, 99.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.855 CPU sec, 4.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12675, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 84, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (96.50, 44.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.36, 99.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.516 CPU sec, 4.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13022, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 115, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (138.89, 93.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.11, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.251 CPU sec, 4.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12790, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 144, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (134.96, 58.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (210.89, 99.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.282 CPU sec, 4.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12896, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (117.14, 61.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (210.96, 99.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.817 CPU sec, 4.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13014, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 124, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (140.94, 80.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.08, 98.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.635 CPU sec, 4.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13153, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 143, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (150.94, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (210.42, 99.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.131 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12767, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (160.06, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (205.42, 94.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.673 CPU sec, 4.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12787, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 116, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (150.97, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (202.74, 93.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.637 CPU sec, 4.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12766, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 122, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (132.65, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (204.55, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.121 CPU sec, 4.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12719, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 103, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (123.26, 67.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (203.73, 93.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.330 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12456, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 98, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (125.28, 81.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (209.49, 98.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.537 CPU sec, 4.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12599, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 132, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (157.25, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (208.47, 97.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.002 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 12676, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 128, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (138.93, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 183, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (209.81, 98.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.454 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12656, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 162, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (167.82, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (210.80, 100.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.803 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12769, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (143.17, 81.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (210.84, 99.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.962 CPU sec, 5.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12817, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 140, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (145.81, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.34, 98.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.513 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12867, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (150.29, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (207.64, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.471 CPU sec, 4.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12778, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (126.74, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (206.51, 96.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.002 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12633, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (157.70, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (208.68, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.786 CPU sec, 4.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12726, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (134.34, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (205.91, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.637 CPU sec, 4.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12805, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 84, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (111.29, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.99, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.856 CPU sec, 4.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12743, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (136.84, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (202.24, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.239 CPU sec, 4.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12567, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (124.61, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (201.96, 91.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.326 CPU sec, 4.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12805, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 116, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (117.37, 55.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (205.48, 95.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.038 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12841, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 156, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (144.65, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (207.29, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.448 CPU sec, 5.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12778, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 144, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (153.00, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.95, 99.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.153 CPU sec, 6.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12905, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (122.08, 55.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (210.04, 99.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.436 CPU sec, 5.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13032, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 152, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (162.90, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (210.15, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.819 CPU sec, 5.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12902, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (123.11, 56.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (208.00, 96.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.540 CPU sec, 4.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12777, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (123.59, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (205.40, 94.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.410 CPU sec, 4.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12718, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 104, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (123.15, 75.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (205.95, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.328 CPU sec, 4.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12930, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 105, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (119.66, 65.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (203.83, 93.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.411 CPU sec, 4.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12844, 7, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 129, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (163.72, 98.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.25, 95.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.285 CPU sec, 4.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 12757, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 134, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (179.50, 116.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (204.51, 94.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.406 CPU sec, 4.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12552, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 97, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (109.56, 67.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (203.97, 94.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.413 CPU sec, 5.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12537, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 125, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (152.56, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (203.53, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.042 CPU sec, 4.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12627, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 137, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (135.24, 61.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (204.13, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.474 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12742, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 123, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (134.80, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.81, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.886 CPU sec, 4.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12851, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 149, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (153.38, 85.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.47, 97.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.813 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12840, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (116.71, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (208.32, 98.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.035 CPU sec, 4.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12785, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 97, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (124.32, 74.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.36, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.127 CPU sec, 4.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12970, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (122.06, 66.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (207.34, 96.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.349 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12857, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 151, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (157.07, 65.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (209.51, 98.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.323 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12855, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (142.48, 100.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (206.97, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.993 CPU sec, 4.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12721, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (127.77, 64.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (204.71, 93.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.920 CPU sec, 4.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12672, 1, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (122.88, 78.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (205.55, 95.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.983 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12639, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 119, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (136.86, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (204.10, 93.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.193 CPU sec, 5.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12708, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (136.83, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (204.55, 93.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.377 CPU sec, 4.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12445, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 127, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (138.97, 69.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (202.42, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.900 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12650, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (121.59, 75.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 177, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (203.34, 94.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.555 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12705, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 99, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (126.28, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.16, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.128 CPU sec, 4.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12663, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (136.12, 89.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (205.61, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.931 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12565, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 132, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (140.20, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (209.50, 98.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.235 CPU sec, 4.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12726, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 121, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (154.66, 89.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (208.81, 98.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.540 CPU sec, 5.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12732, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 121, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (132.76, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (207.87, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.135 CPU sec, 5.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12665, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 107, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (141.11, 80.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.61, 95.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.376 CPU sec, 4.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12781, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 111, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (140.75, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.27, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.569 CPU sec, 4.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12778, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 134, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (168.26, 115.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (203.06, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.233 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12590, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 95, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (119.93, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (204.14, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.281 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12632, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (139.59, 99.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (202.77, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.078 CPU sec, 4.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12590, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 149, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (161.16, 105.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (205.46, 94.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.012 CPU sec, 4.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12978, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 94, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (124.23, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.23, 99.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.208 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12700, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 108, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (117.48, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.23, 99.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.235 CPU sec, 4.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12751, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (110.75, 47.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (211.68, 99.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.846 CPU sec, 4.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12738, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (109.38, 47.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.92, 99.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.859 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12830, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (127.77, 58.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 184, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (210.86, 100.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.959 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12686, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 83, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (117.00, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (207.91, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.591 CPU sec, 4.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12926, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 133, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (161.54, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (210.19, 100.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.314 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12807, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 126, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (156.54, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (207.65, 97.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.617 CPU sec, 5.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12835, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (152.17, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.84, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.735 CPU sec, 3.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12811, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (138.00, 93.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (204.12, 94.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.459 CPU sec, 5.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12653, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 122, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (142.71, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (202.16, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.177 CPU sec, 4.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12508, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 103, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (120.54, 59.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 177, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (198.82, 89.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.316 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12283, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 108, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (124.12, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.22, 96.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.932 CPU sec, 5.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12714, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (125.52, 72.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (211.56, 99.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.327 CPU sec, 4.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12760, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 121, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (156.42, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (214.71, 102.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.753 CPU sec, 5.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12818, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 100, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (154.74, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.34, 100.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.050 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12967, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 128, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (125.91, 62.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (212.84, 101.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.113 CPU sec, 4.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12886, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 136, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (133.76, 60.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (212.30, 100.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.093 CPU sec, 4.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12727, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (122.90, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (213.08, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.159 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12894, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (122.11, 75.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (209.61, 98.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.392 CPU sec, 4.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12931, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (125.74, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.32, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.631 CPU sec, 5.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12936, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (150.71, 99.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (208.06, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.039 CPU sec, 6.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12787, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (141.51, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (204.64, 94.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.279 CPU sec, 5.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12526, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (144.46, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (200.93, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.164 CPU sec, 4.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12391, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 128, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (129.77, 84.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.57, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.281 CPU sec, 4.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12563, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 138, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (153.97, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (207.99, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.077 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12649, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 142, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (151.12, 72.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (214.39, 103.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.252 CPU sec, 4.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12815, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 107, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (127.00, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (213.51, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.591 CPU sec, 4.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12711, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 96, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (137.32, 102.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 184, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (213.69, 102.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.899 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12710, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (126.52, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (212.88, 101.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.639 CPU sec, 4.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12768, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 146, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (169.09, 100.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (211.32, 99.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.745 CPU sec, 4.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12751, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 136, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (152.52, 83.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (207.90, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.010 CPU sec, 4.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12820, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 90, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (111.04, 72.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (210.12, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.916 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12771, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 131, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (147.93, 93.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (209.01, 97.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.363 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12657, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 115, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 211) [M::mem_pestat] mean and std.dev: (109.58, 30.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (205.53, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.586 CPU sec, 5.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12648, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 123, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (148.82, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (202.06, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.083 CPU sec, 4.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12509, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 142, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (140.50, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (202.91, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.399 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12344, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (120.70, 67.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (202.83, 91.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.288 CPU sec, 4.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12457, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 106, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (105.33, 50.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (207.47, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.553 CPU sec, 6.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12613, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 110, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (159.94, 132.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (207.81, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.943 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12790, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (147.82, 77.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (207.08, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.095 CPU sec, 4.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12672, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 132, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (145.50, 93.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.85, 97.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.893 CPU sec, 4.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12816, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 143, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (140.50, 83.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (207.37, 96.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.037 CPU sec, 5.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12742, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (136.41, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.03, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.847 CPU sec, 4.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12914, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 139, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (133.36, 63.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (207.04, 96.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.042 CPU sec, 4.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12773, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 91, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (113.25, 61.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (205.48, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.397 CPU sec, 5.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12686, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 136, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (131.17, 65.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (205.37, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.364 CPU sec, 4.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12855, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (140.64, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (202.81, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.268 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12556, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 124, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (149.40, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (202.72, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.942 CPU sec, 4.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12494, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (120.48, 78.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (198.26, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.323 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12270, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (128.81, 51.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 176, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (199.20, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.964 CPU sec, 5.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12801, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.93, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (208.17, 96.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.983 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12841, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 143, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (170.63, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (210.24, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.601 CPU sec, 4.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12879, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 114, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (109.96, 45.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (211.55, 100.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.787 CPU sec, 4.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12680, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 128, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (158.06, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (210.07, 98.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.743 CPU sec, 4.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12668, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 117, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (119.54, 59.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 184, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (211.32, 99.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.446 CPU sec, 4.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12950, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (142.35, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (210.38, 98.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.966 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12944, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (133.00, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (210.92, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.694 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12813, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 172, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (196.39, 102.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (209.23, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.793 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12743, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 152, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (155.96, 91.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (204.32, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.342 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12686, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (106.87, 53.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 177, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (201.86, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.366 CPU sec, 4.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12599, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 138, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (141.59, 76.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (200.71, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.430 CPU sec, 4.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12346, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 161, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (178.90, 97.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 175, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.89, 86.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.021 CPU sec, 4.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12296, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 154, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (153.62, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 176, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (197.71, 88.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.727 CPU sec, 4.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12719, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (128.41, 68.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 184, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (214.68, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.840 CPU sec, 4.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12755, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (161.07, 103.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (209.46, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.768 CPU sec, 4.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12781, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 180, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (162.65, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (210.52, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.623 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12798, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 152, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (157.54, 77.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (209.01, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.194 CPU sec, 4.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12758, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 162, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (174.57, 101.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (209.66, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.466 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12918, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (158.61, 104.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (209.61, 98.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.983 CPU sec, 4.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12778, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 115, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (129.34, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (210.30, 100.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.669 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12778, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 159, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (160.26, 110.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (205.80, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.682 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12795, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 102, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (126.80, 77.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (205.84, 95.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.240 CPU sec, 4.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 12610, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (122.88, 53.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (205.61, 94.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.930 CPU sec, 4.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12467, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (123.94, 70.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (200.84, 90.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.594 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12157, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 112, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (137.07, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 174, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (196.56, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.280 CPU sec, 5.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12397, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 125, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (139.14, 74.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 176, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (199.86, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.927 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12912, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (163.10, 93.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (203.62, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.526 CPU sec, 4.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12933, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (143.40, 90.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (203.22, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.684 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12704, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 112, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (111.57, 52.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (202.32, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.285 CPU sec, 4.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12772, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (148.92, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.34, 98.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.472 CPU sec, 4.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12853, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 125, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (140.21, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (210.66, 99.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.579 CPU sec, 4.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12801, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (127.91, 62.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (210.74, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.987 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12862, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 137, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (152.61, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (206.55, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.704 CPU sec, 5.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12527, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (121.76, 50.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (206.45, 95.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.367 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12583, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 130, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (144.31, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.29, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.117 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12692, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (132.12, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (209.57, 99.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.880 CPU sec, 4.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12688, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (135.84, 83.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (208.60, 98.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.782 CPU sec, 4.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12770, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (142.00, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (211.23, 100.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.861 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12806, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (172.51, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (207.49, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.745 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12591, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 138, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (143.40, 64.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.28, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.276 CPU sec, 4.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12747, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (155.65, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.95, 93.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.006 CPU sec, 4.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 12895, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (141.88, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (205.45, 95.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.026 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13018, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 157, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (156.39, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (210.76, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.432 CPU sec, 4.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12693, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (110.31, 66.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 183, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (209.89, 99.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.950 CPU sec, 4.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12933, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (140.89, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.49, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.005 CPU sec, 4.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12901, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (140.32, 81.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (204.76, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.216 CPU sec, 3.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12499, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 102, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (134.80, 84.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (207.91, 95.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.927 CPU sec, 4.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12677, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (144.19, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (207.13, 96.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.432 CPU sec, 4.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12884, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (129.38, 81.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (205.05, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.113 CPU sec, 3.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12817, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 118, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (156.35, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (207.62, 97.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.345 CPU sec, 4.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12884, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 113, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (126.91, 68.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (208.08, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.399 CPU sec, 4.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 12831, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (149.51, 87.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (205.25, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.847 CPU sec, 4.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 12688, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 120, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (111.95, 50.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (204.02, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.569 CPU sec, 5.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12694, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (137.86, 78.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 178, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (201.64, 91.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.486 CPU sec, 4.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12821, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 141, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (152.62, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.49, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.583 CPU sec, 4.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12696, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 120, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (130.97, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (211.50, 100.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.819 CPU sec, 4.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12871, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 158, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (151.85, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (208.78, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.962 CPU sec, 4.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12737, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (119.34, 65.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (205.96, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.923 CPU sec, 5.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12658, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (138.76, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (205.53, 94.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.098 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12619, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (129.91, 87.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (207.81, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.138 CPU sec, 5.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12655, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 109, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (118.63, 52.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (206.34, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.530 CPU sec, 5.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 13025, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (137.46, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (207.97, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.660 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12836, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 151, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (173.59, 85.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.36, 98.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.393 CPU sec, 4.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12586, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 135, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (155.42, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (204.34, 93.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.127 CPU sec, 4.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12804, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (130.62, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (204.42, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.879 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12663, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 92, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (111.91, 65.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (202.48, 92.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.648 CPU sec, 4.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12710, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (131.87, 65.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (204.51, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.449 CPU sec, 4.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12698, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (138.61, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (207.54, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.405 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12776, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 153, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (153.92, 86.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (208.10, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.263 CPU sec, 4.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12880, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 130, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (159.58, 92.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (205.64, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.231 CPU sec, 4.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12925, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 159, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (163.14, 79.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (203.91, 93.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.579 CPU sec, 4.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12492, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 96, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (119.48, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 176, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (199.24, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.842 CPU sec, 4.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12748, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (135.32, 58.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (203.41, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.126 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12735, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (148.28, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (204.96, 94.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.684 CPU sec, 4.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12879, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 143, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (166.17, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (206.97, 95.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.066 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12859, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 139, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (144.13, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (203.00, 92.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.106 CPU sec, 4.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12791, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (143.69, 76.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (201.56, 91.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.823 CPU sec, 4.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12688, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (129.89, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (200.83, 90.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.749 CPU sec, 4.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12771, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 84, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (97.67, 57.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (202.43, 91.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.544 CPU sec, 4.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12815, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 131, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (134.41, 77.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.36, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.288 CPU sec, 4.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12782, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 113, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (117.92, 61.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (206.60, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.383 CPU sec, 4.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12781, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (128.50, 81.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (206.77, 96.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.436 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12773, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 144, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (145.73, 70.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.35, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.394 CPU sec, 4.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12498, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (125.81, 62.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (202.49, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.414 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12591, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (135.11, 91.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (201.98, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.657 CPU sec, 4.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 12658, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 115, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (121.21, 47.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (203.84, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.664 CPU sec, 4.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12601, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (151.76, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.33, 95.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.637 CPU sec, 3.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12835, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 153, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (147.73, 74.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (206.03, 95.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.276 CPU sec, 4.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12771, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (142.09, 68.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.59, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.322 CPU sec, 4.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12642, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 90, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (140.11, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (200.40, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.911 CPU sec, 4.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12764, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 133, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (159.97, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (201.33, 91.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.116 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12636, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 114, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (132.11, 77.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.13, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.684 CPU sec, 4.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12662, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 140, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (149.29, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.25, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.159 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12853, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 99, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (119.72, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (205.92, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.083 CPU sec, 4.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12750, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 121, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (152.26, 85.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (204.65, 94.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.900 CPU sec, 4.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12642, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 119, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (133.00, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (204.40, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.093 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12449, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 123, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (151.27, 87.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (205.08, 94.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.228 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12716, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (172.33, 123.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (203.70, 92.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.770 CPU sec, 4.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12714, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (118.12, 50.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (204.65, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.322 CPU sec, 5.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12871, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 100, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (118.17, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (205.12, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.937 CPU sec, 4.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12639, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (130.39, 76.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (202.59, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.663 CPU sec, 4.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 12621, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (167.10, 102.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (203.87, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.736 CPU sec, 5.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12322, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (175.94, 102.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (200.46, 89.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.501 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12385, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 102, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (134.92, 94.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (201.44, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.508 CPU sec, 5.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12794, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 150, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (172.50, 92.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (210.03, 98.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.312 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12757, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 87, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (114.25, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (209.38, 99.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.756 CPU sec, 4.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12777, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 137, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (133.78, 83.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.58, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.551 CPU sec, 4.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12716, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 138, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (156.06, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (207.13, 96.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.179 CPU sec, 5.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12853, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (124.57, 79.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (206.66, 95.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.651 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12850, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (134.81, 76.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.72, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.804 CPU sec, 4.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12821, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 149, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (136.96, 66.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (210.33, 97.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.708 CPU sec, 4.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12763, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 90, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (104.56, 63.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.74, 97.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.031 CPU sec, 4.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12814, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (133.15, 88.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (206.69, 95.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.699 CPU sec, 4.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12688, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (105.48, 63.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (202.34, 91.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.388 CPU sec, 4.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 12583, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 155, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (152.79, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (201.34, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.698 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 12281, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 106, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (130.11, 76.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (199.91, 89.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.413 CPU sec, 6.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12398, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 117, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (136.47, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (206.50, 95.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.999 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12867, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 121, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (136.29, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 183, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (209.76, 99.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.402 CPU sec, 4.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12868, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (126.71, 61.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.26, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.686 CPU sec, 4.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12682, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 94, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (114.13, 65.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (208.65, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.055 CPU sec, 4.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12853, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 78, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (105.62, 79.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (207.72, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.941 CPU sec, 4.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12771, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 140, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (136.18, 54.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (211.24, 99.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.673 CPU sec, 4.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13108, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (137.67, 63.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (212.53, 101.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.289 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12774, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 119, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (122.11, 48.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (211.61, 100.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.569 CPU sec, 4.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12758, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 116, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (121.70, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (210.39, 98.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.045 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12934, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 163, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (168.19, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.28, 93.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.680 CPU sec, 5.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 12654, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (148.22, 92.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (204.91, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.661 CPU sec, 4.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12485, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (129.69, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (201.97, 90.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.271 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 12395, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 107, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (140.74, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (202.62, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.482 CPU sec, 4.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12823, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 134, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (123.86, 68.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (211.18, 99.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.339 CPU sec, 4.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12857, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (156.86, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.58, 98.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.214 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12811, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 126, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (132.59, 58.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (207.06, 95.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.050 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12727, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 128, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (128.53, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (206.50, 95.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.426 CPU sec, 4.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12833, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 102, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (115.79, 63.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (210.26, 98.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.274 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12611, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (151.62, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.28, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.999 CPU sec, 4.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12826, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 175, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (159.37, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (211.49, 100.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.768 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12735, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (132.96, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (209.55, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.333 CPU sec, 4.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12755, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 118, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (139.00, 82.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (203.51, 93.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.464 CPU sec, 4.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12685, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 141, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (154.33, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (200.99, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.060 CPU sec, 5.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12620, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (126.87, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (195.42, 85.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.503 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12184, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 128, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (137.67, 62.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (195.37, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.422 CPU sec, 5.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12526, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 95, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (137.53, 102.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.01, 96.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.175 CPU sec, 4.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12761, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 110, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (146.36, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (205.84, 94.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.441 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12851, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (161.25, 128.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (205.38, 95.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.170 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12750, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (169.46, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (204.70, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.228 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12712, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 102, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (131.55, 82.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (206.39, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.753 CPU sec, 4.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12624, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (133.45, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (209.45, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.824 CPU sec, 4.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12840, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 132, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (139.33, 63.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.14, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.839 CPU sec, 5.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12725, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 92, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (108.80, 57.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.39, 96.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.906 CPU sec, 4.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12723, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (150.50, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (208.82, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.430 CPU sec, 5.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12702, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (137.82, 77.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (201.16, 91.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.091 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12636, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (139.23, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 175, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (198.49, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.815 CPU sec, 4.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12392, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (134.05, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 175, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (195.85, 85.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.300 CPU sec, 4.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12519, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (115.66, 69.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (200.91, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.749 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12941, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 156, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (145.75, 59.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.87, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.620 CPU sec, 4.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12755, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 157, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (168.68, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (206.45, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.583 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12977, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (128.27, 64.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.62, 93.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.310 CPU sec, 4.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12759, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 122, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (129.48, 62.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (203.47, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.241 CPU sec, 4.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12730, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 156, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (171.69, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.99, 96.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.051 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12831, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (155.96, 100.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (206.35, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.026 CPU sec, 4.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12797, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (202.27, 114.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (209.40, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.461 CPU sec, 4.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12778, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (132.12, 66.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.05, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.050 CPU sec, 4.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12761, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 124, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (132.82, 70.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (203.57, 92.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.194 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 12669, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 132, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (152.05, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (203.04, 92.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.903 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12388, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 91, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (98.24, 45.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 176, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (197.37, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.432 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12143, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 140, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (149.63, 96.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 177, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (197.35, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.762 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12817, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 118, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (143.36, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (208.92, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.846 CPU sec, 5.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12844, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 111, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (133.88, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (205.72, 95.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.186 CPU sec, 4.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12829, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 140, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (152.15, 89.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (203.92, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.520 CPU sec, 5.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12715, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 140, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (154.37, 89.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (202.29, 92.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.237 CPU sec, 4.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13007, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 142, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (155.69, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (208.91, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.444 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12882, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 132, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (145.10, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (205.85, 95.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.143 CPU sec, 4.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12801, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (165.28, 126.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (207.99, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.570 CPU sec, 4.702 real sec [M::process] read 27980 sequences (4224980 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12834, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (123.64, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (207.87, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.336 CPU sec, 4.918 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 5516, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 179, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.65, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 27980 reads in 49.358 CPU sec, 2.129 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21166\tSM:UG10S2P174\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21166_HLKNYBGXX_UG10S2P174_R1.fastq.gz 5347_7332_21166_HLKNYBGXX_UG10S2P174_R2.fastq.gz [main] Real time: 1452.565 sec; CPU: 38020.862 sec Finished alignement of UG10S2P174 at Wed Apr 3 08:38:18 2019 Sorting BAM file started at Wed Apr 3 08:38:18 2019 samtools sort -@ 30 -m 4G UG10S2P174_unsorted.bam -o UG10S2P174.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 08:38:18 2019 rm UG10S2P174_unsorted.bam rm 5347_7332_21166_HLKNYBGXX_UG10S2P174_R1.fastq.gz rm 5347_7332_21166_HLKNYBGXX_UG10S2P174_R2.fastq.gz samtools index UG10S2P174.bam Finished BAM file generation of the sample - UG10S2P174 Processing fastq files for the sample - UG10S1P031 Reading 5347_7332_21167_HLKNYBGXX_UG10S1P031_R1.fastq.gz 5347_7332_21167_HLKNYBGXX_UG10S1P031_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21167\tSM:UG10S1P031\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21167_HLKNYBGXX_UG10S1P031_R1.fastq.gz 5347_7332_21167_HLKNYBGXX_UG10S1P031_R2.fastq.gz | samtools view -Sb1 - > UG10S1P031_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 9518, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 98, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (131.57, 86.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (171.71, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.723 CPU sec, 8.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9436, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 189, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (157.91, 54.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (170.68, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.288 CPU sec, 8.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9614, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 165, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (153.20, 63.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (171.77, 79.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.753 CPU sec, 7.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 9559, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (131.94, 75.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.60, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.868 CPU sec, 8.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9579, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 92, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (100.47, 52.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (166.01, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.522 CPU sec, 7.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9610, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (166.45, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 217.444 CPU sec, 7.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9576, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 95, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (112.36, 84.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (166.31, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.054 CPU sec, 7.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9419, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 97, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (42, 157) [M::mem_pestat] mean and std.dev: (96.36, 29.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (165.31, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.525 CPU sec, 9.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9406, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 112, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (155.64, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (170.35, 77.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.082 CPU sec, 9.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9562, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (130.67, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 154, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (172.04, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.844 CPU sec, 7.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 9566, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 121, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (124.79, 65.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (169.96, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.909 CPU sec, 8.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9667, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 176, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (155.25, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (168.40, 77.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.933 CPU sec, 7.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9567, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 119, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (131.00, 101.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (171.39, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.324 CPU sec, 8.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9532, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 138, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (167.82, 105.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (166.90, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.114 CPU sec, 8.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9638, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 83, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (130.00, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (161.41, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.447 CPU sec, 7.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9373, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 94, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (98.93, 58.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (168.82, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.375 CPU sec, 7.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9457, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 213, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (190.58, 111.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (167.01, 74.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.221 CPU sec, 7.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9521, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 144, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (138.46, 62.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (171.43, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.893 CPU sec, 8.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9435, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 105, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (105.75, 40.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (170.98, 79.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.598 CPU sec, 8.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9741, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (120.91, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.40, 77.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.141 CPU sec, 7.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9533, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 143, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (159.73, 81.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (166.23, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.797 CPU sec, 7.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9501, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 133, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (121.54, 60.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (163.49, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.665 CPU sec, 7.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9375, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 102, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (98.06, 47.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.15, 75.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.510 CPU sec, 7.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 9465, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (146.58, 66.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (168.80, 76.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.938 CPU sec, 7.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9581, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (166.01, 73.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 231.349 CPU sec, 8.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9621, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.96, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 220.622 CPU sec, 7.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9712, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (140.53, 114.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (168.96, 76.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.181 CPU sec, 8.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9608, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 158, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (174.56, 97.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (165.34, 73.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.987 CPU sec, 8.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9546, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 195, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (196.73, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.18, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.637 CPU sec, 8.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 9590, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 164, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (156.12, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (164.60, 73.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.455 CPU sec, 8.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9403, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 140, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (151.54, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (164.07, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.060 CPU sec, 7.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9326, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (157.18, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (166.86, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.242 CPU sec, 8.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9528, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 85, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (105.31, 69.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.79, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.777 CPU sec, 8.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9571, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 124, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (144.09, 103.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (172.25, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.327 CPU sec, 7.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9551, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 127, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 191) [M::mem_pestat] mean and std.dev: (125.56, 24.86) [M::mem_pestat] low and high boundaries for proper pairs: (26, 225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.92, 76.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.494 CPU sec, 7.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9582, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 191, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (164.46, 100.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (166.95, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.733 CPU sec, 7.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9348, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 135, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (205.85, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (166.75, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.169 CPU sec, 7.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9598, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 134, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (139.86, 79.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (164.31, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.896 CPU sec, 7.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9295, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 168, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (152.40, 74.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (164.85, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.447 CPU sec, 7.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9584, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 137, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (132.20, 49.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (166.91, 74.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.222 CPU sec, 7.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9467, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (165.39, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 217.411 CPU sec, 7.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 9520, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (165.22, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 217.964 CPU sec, 8.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 9577, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (167.61, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 203.713 CPU sec, 7.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9381, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 171, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (157.22, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (164.80, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.471 CPU sec, 8.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9431, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 176, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (159.70, 87.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (164.90, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.256 CPU sec, 8.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9451, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 112, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (110.42, 43.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (167.00, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.739 CPU sec, 7.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9710, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 98, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (99.78, 28.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (170.01, 78.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.601 CPU sec, 7.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9548, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (169.54, 77.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 214.063 CPU sec, 7.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9611, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (171.19, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 219.436 CPU sec, 7.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 9543, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 101, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (118.05, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (168.16, 77.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.760 CPU sec, 8.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 9657, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (105.31, 63.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (171.77, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.755 CPU sec, 8.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9492, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 91, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (136.23, 105.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (166.50, 73.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.076 CPU sec, 7.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9268, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (133.50, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 149, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (164.05, 71.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.760 CPU sec, 8.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9324, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (139.90, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (167.59, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.985 CPU sec, 8.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9673, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (120.56, 69.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (171.80, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.779 CPU sec, 7.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9698, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 154, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (170.63, 77.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 207.007 CPU sec, 7.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9632, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (170.13, 77.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 221.869 CPU sec, 7.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9796, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (169.51, 77.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 235.580 CPU sec, 8.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 9669, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (136.95, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (169.38, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.392 CPU sec, 8.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9350, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 81, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (123.21, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (164.42, 73.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.176 CPU sec, 7.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9258, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 100, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (119.38, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (161.33, 68.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.851 CPU sec, 7.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9450, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (201.92, 179.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (169.53, 78.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.028 CPU sec, 7.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9584, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 96, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (109.18, 39.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (172.22, 80.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.052 CPU sec, 8.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 9507, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 106, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (137.44, 79.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 154, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.85, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.406 CPU sec, 7.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9506, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 69, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (123.53, 111.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (169.48, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.048 CPU sec, 7.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9467, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 152, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (173.25, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.93, 77.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.569 CPU sec, 8.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9472, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 139, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (136.69, 79.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (165.10, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.947 CPU sec, 7.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9385, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (157.71, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 149, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (164.13, 71.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.106 CPU sec, 7.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9146, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (165.03, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 213.631 CPU sec, 7.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9480, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 184, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (201.64, 141.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.23, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.727 CPU sec, 8.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9431, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (124.38, 63.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (169.16, 76.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.255 CPU sec, 7.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9453, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 165, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (178.69, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (170.44, 78.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.715 CPU sec, 7.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9587, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 142, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (138.92, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (167.50, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.004 CPU sec, 7.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9429, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 84, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (94.37, 41.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (166.78, 73.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.432 CPU sec, 8.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9568, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (126.74, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (163.87, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.110 CPU sec, 7.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9305, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 145, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (133.83, 69.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (162.37, 69.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.925 CPU sec, 7.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9282, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 168, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (171.40, 92.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (165.22, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.814 CPU sec, 8.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9764, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 151, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (113.31, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (170.36, 79.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.478 CPU sec, 8.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9628, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 127, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (94.00, 39.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (171.58, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.056 CPU sec, 8.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9695, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 143, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (151.45, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.73, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.938 CPU sec, 8.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 9546, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 100, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (114.91, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (169.44, 77.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.756 CPU sec, 7.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9512, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 78, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (88.64, 49.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (167.32, 74.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.070 CPU sec, 7.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9344, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (127.30, 80.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (164.09, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.003 CPU sec, 9.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9292, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 122, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (132.64, 44.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (159.46, 66.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.095 CPU sec, 7.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9527, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (169.45, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.817 CPU sec, 8.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9427, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 91, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (126.89, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (167.46, 75.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.238 CPU sec, 7.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9652, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 110, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (127.60, 85.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (167.96, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.437 CPU sec, 8.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9630, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 69, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (71.15, 43.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (169.10, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.763 CPU sec, 7.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9514, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 116, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (113.45, 45.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (169.05, 77.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.199 CPU sec, 7.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 9487, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 152, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (139.00, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (167.15, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.977 CPU sec, 7.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9410, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 88, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (128.33, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (163.68, 71.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.238 CPU sec, 7.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 9321, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (162.69, 70.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 227.782 CPU sec, 7.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9351, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 186, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (213.12, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.44, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.917 CPU sec, 7.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9542, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 115, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (112.77, 58.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (165.17, 73.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.384 CPU sec, 7.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 9528, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 91, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (117.33, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (168.96, 76.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.657 CPU sec, 8.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 9475, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (149.55, 76.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (168.95, 78.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.340 CPU sec, 8.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 9356, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.72, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 218.589 CPU sec, 7.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9421, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.73, 76.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 214.420 CPU sec, 7.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9436, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (170.01, 79.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 232.965 CPU sec, 8.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 9562, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (168.60, 77.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 218.221 CPU sec, 8.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9517, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 120, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (128.06, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (166.51, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.297 CPU sec, 8.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9494, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 85, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.24, 90.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (166.90, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.884 CPU sec, 7.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9526, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 193, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (194.50, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (170.34, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.947 CPU sec, 8.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 9624, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 104, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (98.22, 52.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (166.58, 74.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.257 CPU sec, 7.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9339, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 126, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (142.58, 105.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (168.98, 76.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.654 CPU sec, 8.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9562, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (168.90, 76.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 215.339 CPU sec, 8.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9528, 0, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.99, 76.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 209.872 CPU sec, 8.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9410, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 114, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (150.06, 86.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.44, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.015 CPU sec, 7.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9465, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 125, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (126.55, 51.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (163.56, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.564 CPU sec, 7.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 9645, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 142, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (179.24, 121.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (171.45, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.823 CPU sec, 8.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9475, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (140.20, 95.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.23, 75.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.227 CPU sec, 7.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9512, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 137, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (140.25, 81.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (166.55, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.957 CPU sec, 7.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9588, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 70, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (86.69, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (168.10, 74.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.409 CPU sec, 8.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9552, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 140, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (126.33, 51.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (169.82, 78.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.204 CPU sec, 7.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9443, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 109, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (153.79, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (163.61, 69.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.576 CPU sec, 6.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9419, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (186.71, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (163.24, 70.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.396 CPU sec, 7.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9577, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 151, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (152.50, 104.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.10, 75.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.437 CPU sec, 7.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9476, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.92, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 218.559 CPU sec, 8.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9546, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (149.62, 81.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (165.68, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.971 CPU sec, 8.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9449, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 118, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (129.07, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.88, 68.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.878 CPU sec, 7.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9466, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (165.35, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 216.683 CPU sec, 7.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9664, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 95, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (96.55, 43.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (168.49, 76.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.078 CPU sec, 7.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9448, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 142, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (134.57, 82.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (164.41, 72.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.069 CPU sec, 7.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9411, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 132, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (152.06, 85.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (163.97, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.176 CPU sec, 7.785 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9476, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 111, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (126.73, 80.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (166.78, 76.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.712 CPU sec, 7.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9532, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 121, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (166.36, 146.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (169.53, 76.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.217 CPU sec, 7.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9391, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 87, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (127.31, 83.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (165.37, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.646 CPU sec, 7.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9377, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 120, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (138.29, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (167.15, 74.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.637 CPU sec, 7.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9508, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 99, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (124.36, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (165.86, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.649 CPU sec, 7.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9355, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 147, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (140.71, 107.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (163.57, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.198 CPU sec, 7.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9518, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 145, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (165.73, 69.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (162.29, 68.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.056 CPU sec, 7.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9444, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (167.43, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 215.951 CPU sec, 8.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9531, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (135.46, 93.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (166.08, 74.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.331 CPU sec, 7.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9465, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 73, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (60.00, 24.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.92, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.255 CPU sec, 7.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9512, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (129.47, 76.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (165.45, 74.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.883 CPU sec, 8.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9567, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 130, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (137.57, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (166.90, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.445 CPU sec, 8.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9565, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 168, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (144.27, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.06, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.732 CPU sec, 7.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 9317, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 136, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (136.58, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 149, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (162.92, 69.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.679 CPU sec, 8.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9467, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (167.00, 74.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 209.334 CPU sec, 7.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9562, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (168.61, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 214.325 CPU sec, 7.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 9513, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.32, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 206.818 CPU sec, 7.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9459, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 112, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (122.20, 46.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (169.64, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.489 CPU sec, 9.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9351, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 111, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (102.19, 46.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (171.86, 79.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.264 CPU sec, 7.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9477, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 125, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (184.50, 158.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (170.06, 77.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.156 CPU sec, 7.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9444, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 175, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (174.00, 57.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.32, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.952 CPU sec, 7.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 9005, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (165.13, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 224.955 CPU sec, 8.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9529, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 69, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (64.40, 35.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (169.53, 76.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.758 CPU sec, 8.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9564, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 93, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 179) [M::mem_pestat] mean and std.dev: (88.44, 32.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.71, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.786 CPU sec, 7.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9582, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (135.27, 72.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (169.15, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.063 CPU sec, 7.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9597, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.46, 77.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 237.043 CPU sec, 8.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9509, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 121, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (189.70, 165.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (169.47, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.175 CPU sec, 8.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9499, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 150, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (145.10, 58.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (166.89, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.270 CPU sec, 7.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9440, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 75, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (56.00, 30.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (162.61, 70.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.945 CPU sec, 8.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 9358, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 97, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (100.24, 61.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (166.22, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.923 CPU sec, 8.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9621, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 122, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (124.92, 73.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (168.33, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.764 CPU sec, 7.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9568, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 155, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (152.90, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (168.72, 76.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.233 CPU sec, 9.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9350, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 158, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (179.58, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (170.50, 78.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.163 CPU sec, 7.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9701, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 165, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (175.35, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (170.70, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.944 CPU sec, 7.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9628, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (165.12, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 226.965 CPU sec, 8.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9289, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 100, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (126.08, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (162.85, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.123 CPU sec, 8.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 9174, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (161.14, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 214.082 CPU sec, 7.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9463, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 123, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (136.27, 54.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (166.15, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.348 CPU sec, 7.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9719, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 89, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (119.73, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (162.84, 70.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.784 CPU sec, 7.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 9593, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 104, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (110.08, 54.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (168.92, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.762 CPU sec, 7.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9386, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 125, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 252) [M::mem_pestat] mean and std.dev: (119.42, 38.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (167.77, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.677 CPU sec, 7.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9520, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (151.27, 74.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (169.25, 76.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.592 CPU sec, 7.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 9514, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (166.61, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (164.17, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.783 CPU sec, 7.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 9188, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 79, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (113.45, 57.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.73, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.252 CPU sec, 7.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9535, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (112.76, 59.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (164.36, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.731 CPU sec, 7.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9540, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 149, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (146.50, 87.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.90, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.115 CPU sec, 7.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9489, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 146, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (172.00, 117.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (165.06, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.460 CPU sec, 7.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9473, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 118, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (110.38, 46.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (169.78, 78.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.342 CPU sec, 8.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 9629, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 104, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (118.75, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (168.88, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.450 CPU sec, 7.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9470, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 160, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (148.42, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 151, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (166.96, 74.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.228 CPU sec, 7.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9400, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 116, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (116.67, 47.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.32, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.393 CPU sec, 7.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 9292, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (104.94, 47.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (165.88, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.244 CPU sec, 7.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 9689, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 80, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (79.22, 44.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (165.83, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.939 CPU sec, 8.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9527, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 165, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (160.33, 61.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (166.48, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.644 CPU sec, 7.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 9575, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 117, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (106.09, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (167.38, 74.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.164 CPU sec, 7.208 real sec [M::process] read 58696 sequences (8863096 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 9528, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 103, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (89.15, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (166.40, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.076 CPU sec, 7.670 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 8372, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (168.13, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 58696 reads in 191.807 CPU sec, 6.838 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21167\tSM:UG10S1P031\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21167_HLKNYBGXX_UG10S1P031_R1.fastq.gz 5347_7332_21167_HLKNYBGXX_UG10S1P031_R2.fastq.gz [main] Real time: 1432.694 sec; CPU: 39055.299 sec Finished alignement of UG10S1P031 at Wed Apr 3 09:03:56 2019 Sorting BAM file started at Wed Apr 3 09:03:56 2019 samtools sort -@ 30 -m 4G UG10S1P031_unsorted.bam -o UG10S1P031.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 09:03:56 2019 rm UG10S1P031_unsorted.bam rm 5347_7332_21167_HLKNYBGXX_UG10S1P031_R1.fastq.gz rm 5347_7332_21167_HLKNYBGXX_UG10S1P031_R2.fastq.gz samtools index UG10S1P031.bam Finished BAM file generation of the sample - UG10S1P031 Processing fastq files for the sample - UG10S2P243 Reading 5347_7332_21168_HLKNYBGXX_UG10S2P243_R1.fastq.gz 5347_7332_21168_HLKNYBGXX_UG10S2P243_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21168\tSM:UG10S2P243\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21168_HLKNYBGXX_UG10S2P243_R1.fastq.gz 5347_7332_21168_HLKNYBGXX_UG10S2P243_R2.fastq.gz | samtools view -Sb1 - > UG10S2P243_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12410, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 232, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (188.29, 113.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (173.19, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.518 CPU sec, 5.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12596, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 79, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (130.08, 111.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (176.21, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.270 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12594, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 169, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (198.00, 119.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (179.36, 102.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.408 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12648, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 148, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (164.83, 86.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (175.85, 96.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.165 CPU sec, 5.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12531, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (213.08, 105.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (175.72, 98.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.596 CPU sec, 5.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12529, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 155, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (152.88, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (173.45, 95.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.794 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12584, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 96, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (90.27, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (178.37, 100.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.837 CPU sec, 4.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12660, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (125.39, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (173.27, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.147 CPU sec, 4.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12467, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 104, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (137.62, 69.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (170.99, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.658 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12486, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (166.00, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.65, 93.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.695 CPU sec, 5.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12619, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 111, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (104.77, 58.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (175.05, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.334 CPU sec, 4.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12646, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 122, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (150.77, 87.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.21, 96.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.229 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12643, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 112, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (139.80, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (178.29, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.626 CPU sec, 5.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12542, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 188, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (182.14, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (177.35, 99.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.660 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12474, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.48, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.069 CPU sec, 4.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12559, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (170.93, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.169 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12530, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 150, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (164.68, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (174.47, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.343 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12395, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 102, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (101.78, 54.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (169.28, 91.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.960 CPU sec, 5.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12380, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 161, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (143.08, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (169.98, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.633 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12435, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (170.11, 89.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.895 CPU sec, 4.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12466, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 94, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (107.12, 67.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (174.24, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.664 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12687, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 127, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (130.42, 81.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (178.68, 101.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.706 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12619, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (176.96, 99.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.806 CPU sec, 5.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12424, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 142, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (154.24, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (173.23, 94.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.447 CPU sec, 5.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12510, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 101, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (94.46, 62.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (171.04, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.999 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12480, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 115, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (132.06, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (171.10, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.080 CPU sec, 5.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12460, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (170.56, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.811 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12291, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 173, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (143.36, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (165.87, 86.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.622 CPU sec, 5.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12403, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 141, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (91, 206) [M::mem_pestat] mean and std.dev: (133.22, 18.32) [M::mem_pestat] low and high boundaries for proper pairs: (60, 229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (167.48, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.969 CPU sec, 4.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12520, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (136.27, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (168.95, 89.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.631 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12491, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (189.40, 107.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (173.61, 95.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.070 CPU sec, 5.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12494, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 182, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (188.93, 100.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (174.60, 96.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.342 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12558, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 83, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (118.19, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (174.96, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.135 CPU sec, 4.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12501, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 152, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (197.21, 136.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (173.90, 96.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.363 CPU sec, 4.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12471, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 129, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (145.00, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.91, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.642 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12493, 7, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (169.95, 91.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.757 CPU sec, 5.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12437, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 113, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (153.28, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (169.72, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.968 CPU sec, 4.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12278, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 76, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (91.33, 42.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (162.24, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.807 CPU sec, 5.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12464, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 180, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (131.30, 72.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (170.81, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.452 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12372, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 92, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (101.85, 70.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (175.24, 96.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.365 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12646, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 139, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (177.18, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (172.83, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.728 CPU sec, 4.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12572, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 149, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (162.20, 88.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (171.49, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.866 CPU sec, 6.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12464, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 86, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (123.00, 95.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (172.17, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.966 CPU sec, 5.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12479, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 97, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (98.33, 46.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (169.07, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.318 CPU sec, 5.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12383, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 142, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (138.46, 68.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (173.51, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.471 CPU sec, 4.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12479, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (134.86, 109.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (168.11, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.097 CPU sec, 4.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12432, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (163.31, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.554 CPU sec, 4.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12165, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (166.28, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.303 CPU sec, 5.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12459, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 207, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (208.73, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (174.65, 96.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.587 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12366, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (169.89, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.957 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12397, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (148.53, 108.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (170.35, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.404 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12517, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 140, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (121.09, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (169.87, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.607 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12447, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 203, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (163.83, 76.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (172.37, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.448 CPU sec, 5.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12381, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 128, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (189.91, 154.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (168.85, 89.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.246 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12294, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (142.92, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (167.73, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.026 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12578, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.55, 93.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.865 CPU sec, 4.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12592, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (137.33, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (178.09, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.189 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12510, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 122, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (173.91, 107.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (178.68, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.351 CPU sec, 4.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12616, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 88, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (113.50, 77.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (173.63, 95.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.392 CPU sec, 4.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12687, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (167.25, 111.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.99, 97.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.284 CPU sec, 5.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12471, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 95, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (125.35, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (177.10, 98.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.326 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12574, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 110, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (170.00, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (172.98, 94.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.159 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12410, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 127, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (151.12, 112.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (167.40, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.792 CPU sec, 4.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12246, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 138, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (190.25, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (168.21, 87.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.882 CPU sec, 5.071 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12071, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (133.30, 81.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (162.53, 82.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.208 CPU sec, 5.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12694, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (178.08, 100.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.286 CPU sec, 5.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12580, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 136, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (142.85, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 149, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (181.67, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.999 CPU sec, 4.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12583, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 157, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (182.07, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (181.68, 104.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.245 CPU sec, 5.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12697, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 114, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (154.24, 107.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (181.24, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.626 CPU sec, 4.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12434, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 106, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (133.91, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (180.48, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.944 CPU sec, 4.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12546, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 162, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (166.14, 88.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (173.20, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.159 CPU sec, 4.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12487, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 160, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (172.29, 106.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.07, 93.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.549 CPU sec, 5.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12209, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (161.45, 96.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (166.84, 87.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.748 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12044, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 201, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (209.33, 128.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (163.99, 83.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.061 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12525, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 141, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (193.46, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (177.21, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.750 CPU sec, 4.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12462, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 104, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (125.29, 85.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (178.24, 100.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.765 CPU sec, 5.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12484, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 76, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (99.33, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (177.39, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.221 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12447, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (149.58, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.55, 98.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.138 CPU sec, 4.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12527, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 131, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (124.75, 64.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (176.24, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.213 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12484, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 77, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (120.07, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 147, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (171.73, 91.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.239 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12462, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 252, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (221.57, 130.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (168.59, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.810 CPU sec, 4.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12190, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 102, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (111.60, 40.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (169.02, 89.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.177 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12117, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 140, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (164.70, 79.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (163.67, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.210 CPU sec, 5.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12411, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.05, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.053 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12450, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 91, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (125.46, 105.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (174.15, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.096 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12420, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (125.11, 76.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (172.47, 94.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.519 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12502, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (82.76, 47.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.39, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.644 CPU sec, 4.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12535, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 171, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (113.12, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (170.12, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.044 CPU sec, 4.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12480, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (159.25, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.18, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.987 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12445, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (168.48, 88.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.433 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12328, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 143, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (155.20, 106.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (165.65, 85.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.977 CPU sec, 5.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12093, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (162.64, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.497 CPU sec, 4.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12281, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 215, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (207.08, 149.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (168.90, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.292 CPU sec, 5.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12540, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (173.70, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.213 CPU sec, 5.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12578, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 123, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (149.67, 93.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (178.84, 100.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.893 CPU sec, 5.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12526, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 99, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (124.65, 81.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (177.49, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.799 CPU sec, 4.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12359, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 116, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (125.50, 61.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (178.73, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.759 CPU sec, 4.799 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12701, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (153.50, 103.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (172.42, 94.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.002 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12408, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 87, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (132.40, 105.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.66, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.765 CPU sec, 4.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12592, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (151.56, 117.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (168.29, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.629 CPU sec, 5.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12149, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 133, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (120.20, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (159.74, 77.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.856 CPU sec, 5.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12146, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (164.50, 100.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (169.00, 88.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.915 CPU sec, 5.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12400, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 203, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (188.10, 65.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (175.06, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.574 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12494, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 143, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (168.64, 145.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (177.58, 99.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.551 CPU sec, 5.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12387, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 159, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (159.92, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (173.87, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.866 CPU sec, 5.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12351, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 169, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (134.21, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (174.43, 95.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.972 CPU sec, 4.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12172, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (120.33, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.38, 93.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.079 CPU sec, 5.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12427, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 109, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (138.62, 110.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.33, 92.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.717 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12393, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (121.36, 77.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (164.95, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.560 CPU sec, 4.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12165, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 173, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (192.43, 127.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 141, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (161.26, 81.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.309 CPU sec, 5.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12085, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 128, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (137.50, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 141, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (160.66, 79.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.059 CPU sec, 4.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12433, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 128, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (164.84, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (169.43, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.418 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12322, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 102, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (81.12, 28.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (168.34, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.214 CPU sec, 4.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12524, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 166, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (199.94, 119.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.36, 92.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.766 CPU sec, 5.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12802, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (197.00, 100.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (173.93, 96.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.797 CPU sec, 5.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12638, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 149, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (145.67, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (177.46, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.506 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12420, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 90, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (111.64, 66.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (172.24, 93.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.820 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12421, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 88, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (89.43, 47.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (167.41, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.117 CPU sec, 5.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12467, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 103, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (124.40, 78.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (172.17, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.834 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12374, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 207, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (201.50, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (174.61, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.147 CPU sec, 4.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12427, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 95, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (144.06, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.09, 91.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.323 CPU sec, 5.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12495, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (168.62, 88.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 143.144 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12576, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 139, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (181.46, 123.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (174.43, 95.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.223 CPU sec, 5.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12542, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 200, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (209.90, 151.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (176.27, 98.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.973 CPU sec, 5.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12684, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (180.56, 130.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (176.08, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.550 CPU sec, 5.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12427, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 137, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (165.13, 132.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.23, 92.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.384 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12400, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (168.35, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.269 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12616, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 111, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (121.94, 66.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.51, 97.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.435 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12532, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 123, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (165.69, 96.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (170.41, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.446 CPU sec, 5.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12487, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 155, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (153.92, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (169.69, 90.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.024 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12444, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 108, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (116.05, 56.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (166.65, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.724 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12563, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (162.72, 85.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (173.95, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.429 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12578, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (173.47, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.863 CPU sec, 4.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12414, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 255, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (232.50, 128.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.81, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.548 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12258, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 149, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (239.41, 182.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (172.29, 92.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.748 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12532, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (185.30, 85.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (172.31, 93.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.579 CPU sec, 4.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12534, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 129, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (128.08, 86.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (175.62, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.035 CPU sec, 5.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12490, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 98, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (123.46, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (169.97, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.639 CPU sec, 4.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12480, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (165.71, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (170.75, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.692 CPU sec, 5.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12443, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 189, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (180.60, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (165.85, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.671 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12513, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 101, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (214.64, 199.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (170.06, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.014 CPU sec, 5.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12505, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (154.08, 93.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (169.89, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.997 CPU sec, 5.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12511, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 137, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (165.23, 102.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (170.65, 91.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.811 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12394, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (166.95, 86.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.681 CPU sec, 5.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12436, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (164.58, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.244 CPU sec, 4.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12532, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 186, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (220.14, 159.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (171.19, 91.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.021 CPU sec, 5.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12505, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 147, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (171.95, 133.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (169.61, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.741 CPU sec, 5.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12556, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 109, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (131.23, 73.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (168.19, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.587 CPU sec, 5.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12329, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 90, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (146.00, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (166.46, 85.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.129 CPU sec, 5.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12341, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 108, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (138.58, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (173.59, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.991 CPU sec, 4.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12436, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 147, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (161.47, 109.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.25, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.452 CPU sec, 5.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12516, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 158, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (177.30, 130.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (167.71, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.785 CPU sec, 5.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12216, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (159.20, 91.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (164.11, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.249 CPU sec, 4.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12247, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 188, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (211.06, 149.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (164.31, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.332 CPU sec, 5.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12531, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 79, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (106.93, 57.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (169.86, 90.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.563 CPU sec, 6.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12550, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (165.23, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.22, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.184 CPU sec, 5.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12412, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 121, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (210.00, 166.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (163.67, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.752 CPU sec, 5.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12507, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 135, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (145.57, 87.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (164.65, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.222 CPU sec, 4.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12709, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 133, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (174.47, 116.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (171.19, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.709 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12608, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 129, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (154.00, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (167.45, 87.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.548 CPU sec, 5.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12327, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 97, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (169.00, 141.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (168.82, 89.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.481 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12400, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 120, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (137.20, 81.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (164.02, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.019 CPU sec, 4.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12331, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 140, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (143.45, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (168.93, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.761 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12580, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 129, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (104.89, 31.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (166.87, 87.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.869 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12426, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 141, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (144.07, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (167.31, 87.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.535 CPU sec, 4.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12310, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 105, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (96.00, 42.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (165.79, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.103 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12166, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 192, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (200.89, 123.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 141, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (160.83, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.974 CPU sec, 5.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12210, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (149.57, 88.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (169.19, 89.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.627 CPU sec, 5.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12454, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 150, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (129.09, 54.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (170.84, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.012 CPU sec, 4.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12532, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 158, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (141.00, 70.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (170.28, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.863 CPU sec, 5.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12514, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 91, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (126.00, 60.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (174.42, 96.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.581 CPU sec, 5.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12713, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 153, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744) [M::mem_pestat] mean and std.dev: (241.91, 345.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (178.11, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.258 CPU sec, 4.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12432, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (172.80, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.134 CPU sec, 4.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12618, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 96, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (102.80, 45.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (173.33, 94.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.553 CPU sec, 4.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12346, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (102.00, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (165.77, 85.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.017 CPU sec, 5.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12133, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 164, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (154.50, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (164.74, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.504 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12340, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 89, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (156.36, 152.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (173.98, 95.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.439 CPU sec, 5.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12670, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (137.92, 116.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (178.83, 101.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.754 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12603, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (157.80, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (172.05, 94.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.346 CPU sec, 6.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12427, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 139, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (237.50, 245.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.77, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.600 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12556, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (278.08, 241.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (176.46, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.123 CPU sec, 5.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12537, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 101, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (130.38, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (176.84, 99.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.523 CPU sec, 4.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12563, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 90, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (104.48, 54.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (174.40, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.331 CPU sec, 4.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12435, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 139, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (156.06, 83.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (169.09, 89.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.259 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12256, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 153, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (155.20, 58.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 142, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (162.38, 81.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.672 CPU sec, 4.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12356, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 100, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (96.20, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (173.41, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.581 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12538, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 154, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (173.21, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (175.66, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.151 CPU sec, 5.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12489, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 98, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (131.55, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (171.42, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.522 CPU sec, 4.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12602, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 73, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (129.45, 107.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (177.58, 100.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.061 CPU sec, 4.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12645, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 140, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (138.50, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (178.37, 99.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.709 CPU sec, 5.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12727, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 89, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (101.45, 48.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (177.23, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.881 CPU sec, 5.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12431, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 144, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (166.44, 114.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (167.75, 87.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.214 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12210, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 142, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (121.67, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (162.41, 81.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.322 CPU sec, 5.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12143, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (121.23, 49.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 141, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (156.33, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.052 CPU sec, 4.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12260, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 132, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (133.88, 83.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (166.69, 86.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.921 CPU sec, 4.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12410, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 149, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (151.38, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (170.37, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.743 CPU sec, 5.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12357, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 144, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (151.08, 92.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (167.55, 88.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.654 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12384, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 89, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (131.67, 104.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.33, 91.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.732 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12491, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 152, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (196.18, 118.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.59, 92.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.717 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12367, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 189, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (180.60, 102.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (173.05, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.071 CPU sec, 5.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12561, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (167.52, 87.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.418 CPU sec, 4.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12203, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 104, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (137.77, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 141, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (160.05, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.532 CPU sec, 4.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11924, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 187, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (142.77, 76.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 139, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (156.81, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.561 CPU sec, 5.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12450, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 98, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (85.11, 24.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (171.38, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.996 CPU sec, 4.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12603, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (139.14, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (169.51, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.930 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12517, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 84, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (101.15, 45.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (168.84, 88.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.530 CPU sec, 4.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12573, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 186, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (168.00, 98.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.28, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.135 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12401, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 216, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (199.78, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (170.90, 91.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.548 CPU sec, 4.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12527, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 144, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (187.44, 139.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (176.63, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.936 CPU sec, 6.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12585, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 164, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (214.58, 161.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (169.48, 91.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.745 CPU sec, 4.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12223, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 117, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (92.92, 59.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 143, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (165.20, 85.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.050 CPU sec, 4.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12127, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 129, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (157.31, 108.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 140, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (161.01, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.701 CPU sec, 5.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12582, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (162.13, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (165.85, 86.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.335 CPU sec, 5.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12440, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 94, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (135.48, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (172.52, 94.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.512 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12562, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 136, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (158.27, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (170.36, 91.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.130 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12385, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 188, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (171.00, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (173.97, 96.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.368 CPU sec, 4.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12514, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 102, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (117.07, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (172.08, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.120 CPU sec, 4.780 real sec [M::process] read 5252 sequences (793052 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12489, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 148, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (141.75, 112.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (170.54, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.657 CPU sec, 5.159 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 982, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (171.70, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 5252 reads in 10.332 CPU sec, 0.597 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21168\tSM:UG10S2P243\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21168_HLKNYBGXX_UG10S2P243_R1.fastq.gz 5347_7332_21168_HLKNYBGXX_UG10S2P243_R2.fastq.gz [main] Real time: 1113.425 sec; CPU: 30023.983 sec Finished alignement of UG10S2P243 at Wed Apr 3 09:28:42 2019 Sorting BAM file started at Wed Apr 3 09:28:42 2019 samtools sort -@ 30 -m 4G UG10S2P243_unsorted.bam -o UG10S2P243.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 09:28:42 2019 rm UG10S2P243_unsorted.bam rm 5347_7332_21168_HLKNYBGXX_UG10S2P243_R1.fastq.gz rm 5347_7332_21168_HLKNYBGXX_UG10S2P243_R2.fastq.gz samtools index UG10S2P243.bam Finished BAM file generation of the sample - UG10S2P243 Processing fastq files for the sample - UG10S2P244 Reading 5347_7332_21169_HLKNYBGXX_UG10S2P244_R1.fastq.gz 5347_7332_21169_HLKNYBGXX_UG10S2P244_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21169\tSM:UG10S2P244\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21169_HLKNYBGXX_UG10S2P244_R1.fastq.gz 5347_7332_21169_HLKNYBGXX_UG10S2P244_R2.fastq.gz | samtools view -Sb1 - > UG10S2P244_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12377, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 91, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (109.44, 68.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 198, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (239.27, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 421.258 CPU sec, 14.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11827, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 108, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (131.50, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 200, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (240.61, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 396.652 CPU sec, 14.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13338, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 106, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (142.15, 105.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 197, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (235.18, 132.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 344.363 CPU sec, 11.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12031, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 139, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (149.76, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 202, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (241.74, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 409.906 CPU sec, 14.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13188, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (141.00, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 196, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (234.02, 132.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 315.284 CPU sec, 11.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12669, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (167.59, 94.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 197, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (236.65, 134.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 386.522 CPU sec, 13.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11857, 9, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (145.85, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 198, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (238.56, 135.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 406.200 CPU sec, 14.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13408, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 179, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (186.87, 113.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 192, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (229.61, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 314.801 CPU sec, 10.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11816, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 166, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (204.25, 154.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 200, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (238.91, 136.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 420.321 CPU sec, 14.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 11917, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 143, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (134.54, 98.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 195, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (232.99, 132.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 384.908 CPU sec, 13.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12958, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 91, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (111.10, 60.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 195, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (231.80, 129.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 356.232 CPU sec, 12.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11534, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 124, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (165.18, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 197, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (234.69, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 414.193 CPU sec, 14.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13147, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 136, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (203.81, 194.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 194, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (231.61, 129.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 316.614 CPU sec, 10.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11543, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 125, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (149.32, 72.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 194, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (231.74, 131.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 407.873 CPU sec, 14.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11506, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 118, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (144.94, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 196, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (233.43, 131.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 414.908 CPU sec, 14.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12636, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 199, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (195.88, 108.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 197, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (235.23, 132.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 320.066 CPU sec, 11.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11589, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (157.88, 69.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 199, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (239.97, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 437.018 CPU sec, 14.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12912, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (147.88, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 194, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (234.27, 131.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 372.231 CPU sec, 12.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11930, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 190, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (183.08, 104.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 203, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (242.46, 136.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 391.358 CPU sec, 13.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11743, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 125, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (125.27, 52.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 200, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (240.26, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 431.842 CPU sec, 14.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12886, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 156, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (153.05, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (233.73, 131.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 344.587 CPU sec, 11.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11481, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 208, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422) [M::mem_pestat] mean and std.dev: (222.67, 176.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 199, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (239.90, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 433.851 CPU sec, 14.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12502, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 147, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (137.71, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 195, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (235.67, 132.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 379.221 CPU sec, 13.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11888, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 138, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (152.00, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 196, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (233.23, 130.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 365.421 CPU sec, 12.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11177, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (121.35, 64.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 195, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (234.85, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 461.111 CPU sec, 16.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12807, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 134, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (135.65, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 189, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (225.11, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 338.304 CPU sec, 11.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11219, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 133, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (176.84, 108.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 200, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (239.60, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 422.231 CPU sec, 14.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11550, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (146.46, 86.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 198, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (238.03, 135.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 430.406 CPU sec, 15.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12835, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 141, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (178.12, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 190, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (228.49, 127.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 320.090 CPU sec, 10.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 10842, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 142, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (171.67, 125.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 200, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (240.30, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 456.989 CPU sec, 15.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11996, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 92, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (125.24, 78.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 195, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (232.28, 131.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 370.297 CPU sec, 13.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13487, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 242, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (219.37, 134.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 190, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (226.40, 125.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 292.856 CPU sec, 10.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11965, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 144, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (180.67, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 196, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (236.19, 133.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 422.050 CPU sec, 14.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12389, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 154, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (132.57, 76.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 199, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (237.68, 134.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 392.291 CPU sec, 13.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12603, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 163, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (172.38, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 197, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (236.30, 132.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 369.569 CPU sec, 12.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12365, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 194, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (185.47, 117.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 196, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (237.11, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 372.965 CPU sec, 13.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13289, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (124.90, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (231.53, 129.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 335.383 CPU sec, 11.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12347, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (157.76, 65.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 194, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (234.32, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 366.592 CPU sec, 12.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12859, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 168, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (189.33, 158.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 197, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (236.35, 132.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 363.232 CPU sec, 12.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11912, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (153.74, 86.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (234.01, 132.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 415.961 CPU sec, 14.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11812, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 163, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (170.80, 111.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 193, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (229.93, 129.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 406.957 CPU sec, 14.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12951, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 141, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 270) [M::mem_pestat] mean and std.dev: (131.00, 57.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (230.88, 128.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 337.312 CPU sec, 11.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11572, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 132, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (155.80, 111.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 196, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (232.65, 129.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 439.224 CPU sec, 15.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12463, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 124, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (120.05, 57.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (231.09, 129.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 388.268 CPU sec, 13.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12198, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 100, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (160.83, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 194, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (229.27, 126.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 359.772 CPU sec, 12.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12279, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 253, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (254.93, 174.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 189, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (227.14, 127.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 372.123 CPU sec, 12.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12888, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 183, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (192.44, 129.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 191, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (227.56, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 350.079 CPU sec, 12.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11923, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 165, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (152.62, 123.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 197, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (234.84, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 408.955 CPU sec, 13.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12245, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 139, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (150.50, 96.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 193, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (234.11, 133.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 401.657 CPU sec, 13.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12052, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 158, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (168.45, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 195, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (233.52, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 398.505 CPU sec, 13.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11627, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 131, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (145.92, 77.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 201, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (241.17, 136.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 431.096 CPU sec, 14.522 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12793, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 180, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (191.52, 125.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 194, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (232.02, 129.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 357.267 CPU sec, 12.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12092, 9, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 110, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (122.20, 76.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 199, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (237.25, 133.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 395.161 CPU sec, 13.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12240, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 166, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (166.61, 91.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 194, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (232.07, 130.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 318.585 CPU sec, 11.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11585, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 122, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (158.24, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 188, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (225.79, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 328.517 CPU sec, 11.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11436, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 139, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (157.88, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 198, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (236.64, 132.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 353.472 CPU sec, 12.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12499, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 152, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (200.67, 135.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 188, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (222.41, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 295.544 CPU sec, 9.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11496, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 88, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (123.92, 84.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (232.19, 130.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 447.547 CPU sec, 15.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11731, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 105, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (116.53, 62.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 194, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (232.92, 131.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 399.480 CPU sec, 13.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12189, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 150, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (150.70, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 191, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (227.18, 124.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 361.754 CPU sec, 12.220 real sec [M::process] read 18554 sequences (2801654 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11865, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (199.59, 143.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 196, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (234.16, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 379.497 CPU sec, 13.116 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 3163, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 194, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (233.46, 133.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 18554 reads in 118.028 CPU sec, 4.561 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21169\tSM:UG10S2P244\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21169_HLKNYBGXX_UG10S2P244_R1.fastq.gz 5347_7332_21169_HLKNYBGXX_UG10S2P244_R2.fastq.gz [main] Real time: 811.522 sec; CPU: 23366.320 sec Finished alignement of UG10S2P244 at Wed Apr 3 09:48:19 2019 Sorting BAM file started at Wed Apr 3 09:48:19 2019 samtools sort -@ 30 -m 4G UG10S2P244_unsorted.bam -o UG10S2P244.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 09:48:19 2019 rm UG10S2P244_unsorted.bam rm 5347_7332_21169_HLKNYBGXX_UG10S2P244_R1.fastq.gz rm 5347_7332_21169_HLKNYBGXX_UG10S2P244_R2.fastq.gz samtools index UG10S2P244.bam Finished BAM file generation of the sample - UG10S2P244 Processing fastq files for the sample - UG10S2P237 Reading 5347_7332_21170_HLKNYBGXX_UG10S2P237_R1.fastq.gz 5347_7332_21170_HLKNYBGXX_UG10S2P237_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21170\tSM:UG10S2P237\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21170_HLKNYBGXX_UG10S2P237_R1.fastq.gz 5347_7332_21170_HLKNYBGXX_UG10S2P237_R2.fastq.gz | samtools view -Sb1 - > UG10S2P237_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13291, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.46, 74.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.598 CPU sec, 5.631 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13424, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 142, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (185.33, 164.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (166.78, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.829 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13421, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 113, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (163.75, 128.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (167.11, 77.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.720 CPU sec, 5.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13561, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 90, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (126.29, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (168.52, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.549 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13471, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 90, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (80.07, 43.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (165.03, 76.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.625 CPU sec, 4.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13505, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 76, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (90.71, 48.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (165.40, 77.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.220 CPU sec, 5.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13556, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 130, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (165.47, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.62, 76.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.645 CPU sec, 4.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13405, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 80, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (151.47, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (164.35, 74.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.960 CPU sec, 4.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13290, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 189, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (153.73, 87.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (163.61, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.281 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13478, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 107, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (174.87, 148.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.87, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.470 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13510, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 123, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (139.90, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (166.81, 78.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.530 CPU sec, 5.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13565, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 109, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (130.25, 61.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.69, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.879 CPU sec, 5.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13560, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.77, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.002 CPU sec, 5.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13624, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 157, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (143.88, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (165.43, 77.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.895 CPU sec, 4.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13322, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (146.94, 106.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.99, 76.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.327 CPU sec, 4.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13244, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 143, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (185.79, 111.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.08, 76.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.592 CPU sec, 4.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13560, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 90, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (103.40, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (161.39, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.567 CPU sec, 4.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13184, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.70, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.694 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13484, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 135, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (168.70, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (164.36, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.738 CPU sec, 4.747 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13317, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 105, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (92.90, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (164.81, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.697 CPU sec, 4.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13564, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 130, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (155.33, 134.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (166.58, 78.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.786 CPU sec, 4.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13716, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 167, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (201.82, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.01, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.696 CPU sec, 5.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13451, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (121.14, 56.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (160.83, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.120 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13608, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 112, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (98.64, 56.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (165.21, 76.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.385 CPU sec, 4.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13528, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.77, 72.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.032 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13495, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.64, 70.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.265 CPU sec, 4.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13440, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 115, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (151.80, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.39, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.920 CPU sec, 5.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13400, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 94, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (94.90, 52.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (162.73, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.412 CPU sec, 4.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13690, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (137.00, 61.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (166.14, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.718 CPU sec, 4.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13548, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 177, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (186.56, 127.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.35, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.177 CPU sec, 4.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13477, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 103, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (91.92, 45.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (162.77, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.825 CPU sec, 4.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13554, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.39, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.386 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13521, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.45, 85.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.52, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.623 CPU sec, 4.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13475, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 88, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (149.64, 93.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (164.02, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.807 CPU sec, 4.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13246, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 81, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (95.69, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (161.43, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.265 CPU sec, 4.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13445, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 135, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (148.36, 122.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.64, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.559 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13408, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (132.48, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.03, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.168 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13537, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 100, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (103.27, 56.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.50, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.824 CPU sec, 4.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13725, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 104, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (115.47, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.70, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.510 CPU sec, 4.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13619, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 64, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (97.14, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.27, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.081 CPU sec, 4.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13571, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 86, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (99.92, 46.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.14, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.633 CPU sec, 4.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13432, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 95, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (119.40, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (164.45, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.832 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13251, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (102.89, 52.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.32, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.723 CPU sec, 4.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13468, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (109.56, 54.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (159.39, 70.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.398 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13453, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 134, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (167.94, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.42, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.778 CPU sec, 4.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13349, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 119, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (135.92, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.20, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.051 CPU sec, 3.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13405, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 137, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (165.61, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.61, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.778 CPU sec, 4.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13595, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (123.23, 61.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (161.71, 72.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.338 CPU sec, 4.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13592, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 115, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (140.94, 96.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (160.97, 71.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.126 CPU sec, 4.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13354, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 149, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (187.87, 135.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (161.53, 72.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.572 CPU sec, 4.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13648, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 114, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (115.45, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.52, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.111 CPU sec, 4.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13468, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (135.23, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (165.60, 77.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.769 CPU sec, 5.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13533, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 140, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (202.21, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (167.69, 79.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.061 CPU sec, 4.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13626, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 143, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (159.13, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (167.31, 79.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.547 CPU sec, 4.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13498, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 89, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (96.56, 60.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (166.70, 78.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.905 CPU sec, 4.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13461, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 106, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (100.09, 47.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.33, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.099 CPU sec, 4.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13600, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (103.92, 50.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.44, 73.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.602 CPU sec, 5.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13460, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 86, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (118.32, 66.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (165.76, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.329 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13167, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 102, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (120.86, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.72, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.106 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13461, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (127.36, 73.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (167.90, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.517 CPU sec, 5.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13328, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 116, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (125.73, 75.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (168.37, 79.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.537 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13694, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 122, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (155.79, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.16, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.688 CPU sec, 5.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13506, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 141, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (131.75, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (166.61, 77.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.793 CPU sec, 5.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13399, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 110, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (139.94, 83.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.21, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.485 CPU sec, 5.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13481, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 151, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (140.89, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.06, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.697 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13557, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 86, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (118.32, 83.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.76, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.325 CPU sec, 5.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13223, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 115, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (136.83, 81.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.82, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.959 CPU sec, 5.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13153, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 88, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (94.82, 61.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.54, 74.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.877 CPU sec, 5.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13680, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 109, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (99.60, 53.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (169.05, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.380 CPU sec, 5.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13578, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (157.61, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.61, 77.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.941 CPU sec, 5.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13476, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 55, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (90.25, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (167.70, 78.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.746 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13423, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 92, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (105.46, 68.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.43, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.053 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13301, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 178, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (158.36, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.00, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.452 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13515, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (132.06, 64.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.52, 73.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.516 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13135, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 98, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (129.67, 94.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (161.95, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.368 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13154, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (146.06, 87.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (160.33, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.821 CPU sec, 6.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13632, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 109, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (136.33, 86.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (165.58, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.812 CPU sec, 5.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13443, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 89, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (100.94, 65.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.62, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.149 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13384, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (170.83, 107.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (168.00, 79.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.193 CPU sec, 4.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13446, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (97.88, 26.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.56, 77.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.414 CPU sec, 7.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13588, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (164.10, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.603 CPU sec, 5.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13562, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 93, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (118.08, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.33, 73.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.022 CPU sec, 5.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13576, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 209, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (175.67, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (160.36, 69.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.020 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13351, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 95, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (114.92, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (161.15, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.333 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13298, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 93, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (121.73, 64.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.09, 71.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.986 CPU sec, 4.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13665, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 129, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (117.20, 36.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (166.63, 78.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.430 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13394, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 80, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (105.82, 59.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (167.11, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.615 CPU sec, 4.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13375, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 123, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (153.00, 112.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (167.26, 78.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.500 CPU sec, 5.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13543, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 199, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (305.18, 345.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (167.50, 79.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.882 CPU sec, 4.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13689, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 99, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (104.24, 64.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (164.46, 76.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.769 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13436, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.73, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.049 CPU sec, 5.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13413, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 69, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (135.36, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (160.41, 70.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.260 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13176, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (159.01, 69.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.137 CPU sec, 5.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13501, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 107, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (151.47, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (168.13, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.012 CPU sec, 4.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13444, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 106, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (103.62, 40.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.19, 76.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.551 CPU sec, 6.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13449, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 104, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (119.79, 44.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.51, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.344 CPU sec, 6.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13534, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 100, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (129.75, 80.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.71, 73.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.162 CPU sec, 5.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13508, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 157, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (173.17, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.59, 74.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.125 CPU sec, 5.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13426, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 85, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (106.75, 66.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.60, 73.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.953 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13245, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 191, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (177.77, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (161.84, 71.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.681 CPU sec, 5.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13043, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 74, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (80.46, 30.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (155.52, 65.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.794 CPU sec, 7.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13546, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 142, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (140.00, 101.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.50, 74.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.142 CPU sec, 5.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13499, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 104, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (159.32, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.39, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.231 CPU sec, 5.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13410, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 141, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (153.38, 70.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (164.84, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.461 CPU sec, 5.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13565, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 110, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (140.44, 104.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.96, 77.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.897 CPU sec, 6.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13551, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 189, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (180.71, 100.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (165.40, 76.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.964 CPU sec, 5.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13380, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 133, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (143.29, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.50, 74.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.058 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13565, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (126.33, 86.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (164.03, 75.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.250 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13419, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 204, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (188.27, 99.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.03, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.681 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13503, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 106, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (99.10, 45.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.78, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.222 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13446, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 65, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (96.14, 59.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.72, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.554 CPU sec, 5.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13513, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 117, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (176.00, 110.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (164.11, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.745 CPU sec, 5.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13656, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 96, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (85.12, 36.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (164.32, 74.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.782 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13582, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 128, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (162.54, 123.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (167.31, 78.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.065 CPU sec, 5.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13465, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 179, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (169.53, 87.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.86, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.369 CPU sec, 5.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13400, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (117.64, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.92, 74.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.407 CPU sec, 5.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13677, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 105, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (111.85, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (163.79, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.443 CPU sec, 5.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13643, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (110.00, 81.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.89, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.436 CPU sec, 5.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13562, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 181, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (168.50, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.41, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.934 CPU sec, 4.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13395, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 58, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (103.90, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.77, 70.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.600 CPU sec, 5.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13616, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 155, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (129.56, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.43, 76.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.212 CPU sec, 4.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13662, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 95, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (111.00, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (165.35, 77.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.820 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13523, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 68, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (76.54, 45.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.86, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.471 CPU sec, 5.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13338, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.48, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.004 CPU sec, 6.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13537, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 165, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (217.07, 178.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (163.76, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.970 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13314, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.98, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.213 CPU sec, 5.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13574, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 267, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (267.21, 226.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (161.41, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.007 CPU sec, 5.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13526, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (175.00, 87.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.04, 72.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.365 CPU sec, 4.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13442, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 96, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (124.00, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.36, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.992 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13524, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 95, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (106.86, 67.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.59, 74.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.857 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13570, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 68, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (111.14, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.27, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.124 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13397, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 164, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (142.45, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (158.52, 67.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.864 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13426, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (161.83, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.505 CPU sec, 6.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13356, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (140.38, 101.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (162.16, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.858 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13326, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 88, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (124.77, 104.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (164.36, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.043 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13530, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 139, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (148.60, 78.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (163.67, 73.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.001 CPU sec, 5.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13551, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 169, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (264.92, 227.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.32, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.518 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13540, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 79, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (79.14, 33.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.43, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.594 CPU sec, 5.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13507, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 99, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (120.33, 59.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (165.86, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.348 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13401, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (159.96, 68.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.530 CPU sec, 4.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13481, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 126, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (122.06, 65.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.61, 72.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.029 CPU sec, 5.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13454, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 122, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (112.56, 65.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (162.95, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.092 CPU sec, 5.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13624, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (150.23, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (162.21, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.957 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13390, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 98, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (113.07, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (156.73, 66.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.433 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13328, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 98, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (126.93, 93.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (162.20, 71.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.866 CPU sec, 5.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13466, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (112.08, 56.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (161.38, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.482 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13471, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 95, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (94.77, 60.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.22, 73.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.213 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13352, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (113.11, 62.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (160.45, 70.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.702 CPU sec, 5.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13432, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 149, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (178.47, 143.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.50, 72.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.058 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13469, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 82, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (116.09, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (164.81, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.129 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13308, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 173, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (177.33, 133.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.53, 70.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.101 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13471, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (155.65, 114.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (160.02, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.850 CPU sec, 5.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13162, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 128, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (122.00, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.43, 71.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.907 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13312, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 189, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (172.50, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.94, 77.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.338 CPU sec, 5.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13402, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (110.58, 58.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (166.14, 77.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.865 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13604, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (126.74, 71.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.72, 72.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.864 CPU sec, 5.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13556, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 125, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (127.00, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.48, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.247 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13452, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 192, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (161.60, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (164.19, 74.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.151 CPU sec, 4.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13712, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 140, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (138.88, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (162.88, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.994 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13241, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 114, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (141.24, 104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.45, 70.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.797 CPU sec, 6.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13277, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 119, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (130.36, 62.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (159.52, 69.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.889 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13462, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 151, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (169.89, 80.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.512 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13591, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (125.36, 104.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.44, 75.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.709 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13485, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 171, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (132.43, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.58, 76.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.455 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13589, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 109, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (113.36, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.74, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.016 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13429, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 193, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (130.44, 77.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (165.28, 76.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.965 CPU sec, 5.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13514, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (238.00, 220.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.76, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.198 CPU sec, 5.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13448, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (163.27, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.32, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.966 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13250, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (162.19, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.494 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13497, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 114, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (133.29, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (165.54, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.174 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13552, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 90, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 185) [M::mem_pestat] mean and std.dev: (89.00, 28.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.28, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.287 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13585, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 123, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (117.77, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.23, 74.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.059 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13458, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 101, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (127.55, 89.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.35, 77.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.188 CPU sec, 4.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13443, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (156.76, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (166.64, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.096 CPU sec, 5.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13574, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (131.16, 80.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.85, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.607 CPU sec, 4.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13365, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 129, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (156.57, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (160.31, 69.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.286 CPU sec, 5.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13175, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 141, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (171.00, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (155.23, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.928 CPU sec, 5.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13265, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 94, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (79.40, 42.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (159.56, 69.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.200 CPU sec, 5.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13478, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 108, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (143.62, 84.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (161.79, 71.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.712 CPU sec, 5.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13510, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 75, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (99.42, 57.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (160.74, 70.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.860 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13548, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 127, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (126.53, 97.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.62, 75.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.379 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13624, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.62, 75.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.512 CPU sec, 5.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13589, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (171.77, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (160.70, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.314 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13462, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (103.79, 53.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (157.91, 67.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.673 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13460, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (131.14, 62.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (155.91, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.530 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13295, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (160.98, 71.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.418 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13454, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (106.88, 55.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (165.22, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.420 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13468, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 116, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (103.00, 60.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.84, 74.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.441 CPU sec, 4.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13603, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 166, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (173.17, 109.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (161.80, 71.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.381 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13365, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 105, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (100.42, 44.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (163.23, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.106 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 111, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (134.38, 83.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (167.50, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.416 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13409, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 211, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (183.31, 134.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (160.68, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.983 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13431, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 122, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (157.73, 108.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (160.94, 70.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.163 CPU sec, 4.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13420, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 75, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (82.83, 40.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (159.71, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.322 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13576, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (190.29, 143.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.85, 76.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.143 CPU sec, 5.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13536, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 132, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (152.71, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (163.57, 75.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.579 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13609, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 91, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (132.08, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.94, 74.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.511 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13437, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 101, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (185.73, 164.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (166.20, 77.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.564 CPU sec, 5.807 real sec [M::process] read 13042 sequences (1969342 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13369, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 149, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (139.07, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (164.96, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.938 CPU sec, 6.959 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 2687, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.62, 74.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 13042 reads in 29.477 CPU sec, 1.643 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21170\tSM:UG10S2P237\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21170_HLKNYBGXX_UG10S2P237_R1.fastq.gz 5347_7332_21170_HLKNYBGXX_UG10S2P237_R2.fastq.gz [main] Real time: 1037.434 sec; CPU: 27341.216 sec Finished alignement of UG10S2P237 at Wed Apr 3 10:02:13 2019 Sorting BAM file started at Wed Apr 3 10:02:13 2019 samtools sort -@ 30 -m 4G UG10S2P237_unsorted.bam -o UG10S2P237.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 10:02:13 2019 rm UG10S2P237_unsorted.bam rm 5347_7332_21170_HLKNYBGXX_UG10S2P237_R1.fastq.gz rm 5347_7332_21170_HLKNYBGXX_UG10S2P237_R2.fastq.gz samtools index UG10S2P237.bam Finished BAM file generation of the sample - UG10S2P237 Processing fastq files for the sample - UG10S2P248 Reading 5347_7332_21171_HLKNYBGXX_UG10S2P248_R1.fastq.gz 5347_7332_21171_HLKNYBGXX_UG10S2P248_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21171\tSM:UG10S2P248\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21171_HLKNYBGXX_UG10S2P248_R1.fastq.gz 5347_7332_21171_HLKNYBGXX_UG10S2P248_R2.fastq.gz | samtools view -Sb1 - > UG10S2P248_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13466, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (180.00, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.14, 83.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.881 CPU sec, 5.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13446, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (124.46, 86.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.43, 86.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.103 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13595, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 90, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (95.17, 57.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (184.05, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.760 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13688, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 145, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (146.26, 90.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.75, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.990 CPU sec, 3.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13748, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 182, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (161.58, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.13, 86.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.571 CPU sec, 5.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13656, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (140.05, 72.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (183.85, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.784 CPU sec, 4.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13580, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (106.00, 45.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.67, 86.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.146 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13686, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 72, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (85.62, 53.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.93, 84.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.334 CPU sec, 4.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13483, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 104, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (93.77, 51.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (177.90, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.670 CPU sec, 4.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13567, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (135.48, 93.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.89, 85.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.693 CPU sec, 4.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13653, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 80, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (93.31, 64.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (178.78, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.370 CPU sec, 4.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13645, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (150.72, 120.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.00, 83.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.060 CPU sec, 5.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13646, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (111.85, 36.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.98, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.569 CPU sec, 4.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13362, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 119, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (128.21, 58.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.13, 84.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.778 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13556, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 90, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (110.47, 74.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.07, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.693 CPU sec, 5.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13757, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 129, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (126.08, 59.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.98, 84.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.207 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13686, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 100, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (91.30, 45.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.01, 84.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.082 CPU sec, 4.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13584, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 109, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (114.80, 52.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (180.85, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.576 CPU sec, 4.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13699, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 176, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (185.94, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (182.77, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.108 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13647, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 94, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (119.56, 61.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.30, 85.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.017 CPU sec, 4.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13585, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (114.32, 50.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (182.79, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.802 CPU sec, 4.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13744, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 143, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 242) [M::mem_pestat] mean and std.dev: (126.73, 39.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (182.74, 87.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.177 CPU sec, 3.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13600, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (148.67, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.71, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.124 CPU sec, 4.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13615, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (126.38, 60.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.74, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.981 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13750, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 112, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (146.89, 79.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (177.76, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.750 CPU sec, 5.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13473, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 121, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (123.72, 77.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.04, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.663 CPU sec, 5.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13419, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 157, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (173.14, 96.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.32, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.112 CPU sec, 4.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13477, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 157, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (141.24, 77.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.04, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.374 CPU sec, 4.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13421, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 159, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (155.85, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.84, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.888 CPU sec, 4.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13616, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 99, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (153.42, 150.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.07, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.203 CPU sec, 4.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13570, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 108, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (119.71, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.00, 84.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.227 CPU sec, 4.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13664, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (115.09, 48.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.62, 86.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.582 CPU sec, 4.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13602, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 79, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (92.00, 59.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.64, 85.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.854 CPU sec, 4.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13524, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 128, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (197.28, 169.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.67, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.114 CPU sec, 4.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13534, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (122.05, 73.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (180.59, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.562 CPU sec, 4.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13616, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (146.91, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.03, 83.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.005 CPU sec, 5.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13604, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (116.19, 59.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.23, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.395 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13677, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 147, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (130.43, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (178.98, 83.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.869 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13454, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 133, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (138.18, 56.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (176.54, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.685 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13557, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 84, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (106.33, 61.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (179.51, 84.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.952 CPU sec, 4.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13658, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 163, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (174.19, 74.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.95, 85.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.939 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13652, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 86, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (121.14, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.74, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.544 CPU sec, 5.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13622, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 153, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (154.07, 61.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.76, 86.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.625 CPU sec, 4.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13719, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (158.75, 79.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.74, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.102 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13439, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 97, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (106.58, 60.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 161, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (181.09, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.210 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13539, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 158, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (176.27, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.83, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.836 CPU sec, 4.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13624, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 196, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (197.31, 111.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (180.84, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.111 CPU sec, 4.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13543, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 154, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (123.90, 57.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (177.85, 81.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.356 CPU sec, 4.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13678, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 107, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (128.29, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (176.71, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.691 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13754, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 112, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (115.19, 36.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.01, 83.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.338 CPU sec, 4.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13534, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (157.12, 109.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (177.18, 81.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.718 CPU sec, 4.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13486, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 135, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (149.50, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (175.33, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.171 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13330, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (123.36, 59.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (175.88, 81.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.172 CPU sec, 4.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13441, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 199, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (195.50, 66.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.44, 83.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.811 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13741, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (178.89, 83.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.677 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13621, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 70, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (84.58, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (182.86, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.796 CPU sec, 4.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13585, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 145, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (159.48, 101.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.63, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.827 CPU sec, 4.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13622, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 113, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (147.50, 103.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.77, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.330 CPU sec, 4.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13671, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 106, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (111.15, 59.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.73, 85.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.722 CPU sec, 4.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13550, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 114, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (102.14, 36.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.46, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.562 CPU sec, 4.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13441, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (128.52, 70.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.44, 82.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.458 CPU sec, 4.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13523, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 108, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (101.94, 52.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (178.58, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.076 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13657, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 85, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (106.75, 71.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (176.97, 81.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.827 CPU sec, 4.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13572, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 146, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (166.15, 85.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.25, 84.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.905 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13390, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 94, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (124.60, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (177.96, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.355 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13486, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 100, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (104.29, 49.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (176.72, 79.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.277 CPU sec, 4.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13463, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (161.40, 93.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.06, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.858 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13591, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 90, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (132.15, 103.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (178.95, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.091 CPU sec, 4.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13606, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (149.70, 102.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.12, 85.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.602 CPU sec, 4.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13474, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 137, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (135.35, 55.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.19, 84.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.678 CPU sec, 4.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13387, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 121, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (147.63, 104.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.25, 83.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.316 CPU sec, 4.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13540, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 123, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (101.67, 42.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.37, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.029 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13576, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (101.28, 35.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.13, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.703 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13498, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 135, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (132.65, 86.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (177.90, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.941 CPU sec, 4.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13421, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 90, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (106.11, 50.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.66, 81.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.266 CPU sec, 4.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13676, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 117, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (135.29, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.47, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.804 CPU sec, 3.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13385, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 90, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (85.90, 44.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (177.02, 80.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.551 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13604, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 95, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (129.53, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.24, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.929 CPU sec, 4.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13485, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 94, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (122.69, 108.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.16, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.045 CPU sec, 4.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13512, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 81, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (117.12, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.48, 86.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.370 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13715, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 134, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (142.79, 73.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (182.81, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.527 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13597, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 106, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (103.47, 57.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.11, 86.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.501 CPU sec, 4.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13623, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 159, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (174.09, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (181.23, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.324 CPU sec, 4.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13632, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 96, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (131.00, 75.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.73, 86.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.675 CPU sec, 4.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13597, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (134.36, 58.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (182.70, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.588 CPU sec, 4.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13753, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 180, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (172.25, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.02, 87.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.829 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13604, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 97, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (92.26, 48.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.31, 83.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.095 CPU sec, 4.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13501, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (130.32, 56.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.16, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.564 CPU sec, 4.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13320, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 106, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (116.57, 65.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (174.77, 78.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.590 CPU sec, 5.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13142, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (172.92, 127.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 155, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (173.88, 77.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.822 CPU sec, 5.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13574, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 68, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (125.18, 108.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.37, 87.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.580 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13613, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 170, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (125.11, 93.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (184.49, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.838 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13447, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 165, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.90, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.63, 87.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.069 CPU sec, 4.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13745, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 168, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (173.08, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (187.39, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.670 CPU sec, 5.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13676, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 163, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (173.88, 114.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.05, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.687 CPU sec, 4.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13573, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 98, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (126.93, 78.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.33, 87.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.845 CPU sec, 5.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13579, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 62, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (96.18, 52.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (184.36, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.485 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13638, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 83, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (80.19, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.24, 87.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.285 CPU sec, 4.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13633, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 103, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (124.83, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.81, 86.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.299 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13477, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 108, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (106.00, 58.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (178.15, 81.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.854 CPU sec, 4.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13330, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 85, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (105.25, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (175.79, 79.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.860 CPU sec, 4.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13232, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 146, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (143.93, 90.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (175.80, 80.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.866 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13168, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.10, 81.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 125.985 CPU sec, 4.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13474, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 99, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (103.67, 47.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.49, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.126 CPU sec, 4.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13650, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 134, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (112.75, 55.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (183.69, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.146 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13625, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 116, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (122.22, 57.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (184.43, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.541 CPU sec, 4.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13572, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (139.58, 105.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.83, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.757 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13679, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 95, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (142.60, 109.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.38, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.930 CPU sec, 4.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13582, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 103, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (117.10, 68.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (183.38, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.228 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13543, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 143, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (86, 211) [M::mem_pestat] mean and std.dev: (141.36, 24.96) [M::mem_pestat] low and high boundaries for proper pairs: (42, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.33, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.228 CPU sec, 4.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13718, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 123, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (129.63, 60.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.53, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.067 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13618, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 128, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (135.47, 68.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (178.89, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.304 CPU sec, 4.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13434, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 92, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (99.67, 47.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.16, 81.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.136 CPU sec, 4.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13234, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 127, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (114.95, 58.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (174.64, 79.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.664 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13159, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (140.43, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (174.40, 78.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.083 CPU sec, 5.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13241, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 100, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (111.65, 50.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (178.86, 81.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.663 CPU sec, 4.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13475, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 107, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (104.18, 49.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (182.60, 85.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.403 CPU sec, 5.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13661, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (117.50, 56.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (179.09, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.176 CPU sec, 4.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13410, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 217, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (186.18, 132.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.55, 85.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.916 CPU sec, 4.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13488, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 192, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (178.38, 146.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (179.59, 84.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.135 CPU sec, 4.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13448, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 159, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (195.54, 129.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.91, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.629 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13636, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 106, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (113.92, 53.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.36, 87.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.689 CPU sec, 4.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13640, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 106, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (145.29, 95.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.28, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.826 CPU sec, 4.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13591, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 134, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (121.19, 56.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.87, 83.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.565 CPU sec, 4.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13588, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 129, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (134.10, 63.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.79, 81.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.300 CPU sec, 3.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13422, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (139.26, 87.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (175.18, 78.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.383 CPU sec, 5.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13432, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (125.00, 66.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (175.58, 79.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.610 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13221, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 130, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (139.55, 70.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 154, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.76, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.958 CPU sec, 4.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13336, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 84, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (93.07, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.28, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.226 CPU sec, 4.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13600, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 100, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (97.07, 49.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.49, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.907 CPU sec, 5.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13513, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (138.42, 56.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (185.26, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.088 CPU sec, 4.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13476, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 78, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (81.82, 35.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (183.02, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.219 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13446, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 125, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (118.50, 57.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.53, 85.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.629 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13512, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 99, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (132.86, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (184.71, 88.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.085 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13599, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (125.29, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.61, 86.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.393 CPU sec, 4.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13672, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (179.82, 85.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.871 CPU sec, 4.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13499, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 79, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (111.87, 63.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.57, 84.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.364 CPU sec, 5.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13608, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 97, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (107.61, 42.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.22, 82.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.654 CPU sec, 4.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13581, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 146, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (166.71, 112.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (175.48, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.581 CPU sec, 4.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13359, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 116, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (137.48, 93.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 154, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (173.66, 77.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.112 CPU sec, 4.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13101, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 86, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (111.29, 65.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 154, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (171.03, 75.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.739 CPU sec, 5.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13463, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 155, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (167.25, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.35, 84.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.793 CPU sec, 4.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13445, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 141, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (117.40, 41.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.84, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.044 CPU sec, 4.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13525, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (147.40, 83.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.70, 85.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.217 CPU sec, 4.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13401, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 140, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (163.67, 104.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (183.44, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.179 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13584, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 122, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (120.45, 71.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (185.25, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.416 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13464, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 112, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (128.43, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.38, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.242 CPU sec, 4.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13419, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (129.00, 81.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (179.16, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.220 CPU sec, 4.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13621, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 142, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (133.36, 60.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.99, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.528 CPU sec, 5.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13578, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (177.58, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.026 CPU sec, 4.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13617, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 152, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (145.24, 49.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (179.04, 82.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.708 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13469, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 122, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (140.22, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (175.55, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.542 CPU sec, 5.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13351, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 80, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (110.20, 76.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (174.48, 78.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.055 CPU sec, 4.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13150, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 100, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (97.69, 41.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 154, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.95, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.876 CPU sec, 4.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13482, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (153.13, 109.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.83, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.927 CPU sec, 4.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13621, 0, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.25, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.144 CPU sec, 5.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13540, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 136, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (168.21, 110.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.64, 83.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.546 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13578, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.18, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.305 CPU sec, 4.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13716, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (143.92, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.23, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.844 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13798, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 88, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (119.64, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.92, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.208 CPU sec, 4.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13798, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 118, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (137.18, 65.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.97, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.325 CPU sec, 4.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13766, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (149.91, 99.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (180.12, 84.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.142 CPU sec, 4.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13561, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 109, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (137.44, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.84, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.594 CPU sec, 4.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13572, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (122.72, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.55, 84.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.126 CPU sec, 4.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13489, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 156, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (141.13, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (182.70, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.080 CPU sec, 5.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13487, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.74, 85.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.926 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13494, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 98, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (124.15, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (179.60, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.340 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13673, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 117, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (139.24, 64.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (178.19, 82.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.911 CPU sec, 4.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13645, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (151.14, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.06, 81.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.164 CPU sec, 3.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13618, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (136.29, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.02, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.922 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13631, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 140, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (139.67, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (182.06, 86.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.979 CPU sec, 4.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13448, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 108, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (131.47, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (183.50, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.801 CPU sec, 5.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13653, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 180, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (161.60, 79.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.35, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.670 CPU sec, 4.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13767, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 143, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (176.54, 114.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.47, 83.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.970 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13390, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 106, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (110.19, 45.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.14, 85.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.349 CPU sec, 4.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13429, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 111, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (122.00, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.62, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.658 CPU sec, 4.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13647, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (124.70, 81.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.26, 84.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.102 CPU sec, 4.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13620, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (134.56, 55.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.83, 85.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.485 CPU sec, 4.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13802, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 129, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (158.83, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (181.02, 84.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.789 CPU sec, 4.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13570, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 122, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (150.47, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.69, 83.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.136 CPU sec, 5.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13536, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 85, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (110.59, 65.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (176.63, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.918 CPU sec, 4.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13432, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 211, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (173.58, 89.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (174.03, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.298 CPU sec, 5.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13636, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 176, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (191.00, 119.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.45, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.491 CPU sec, 4.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13622, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 81, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (84.88, 38.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.80, 86.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.935 CPU sec, 3.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13594, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (117.50, 69.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.75, 85.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.801 CPU sec, 4.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13640, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (178.90, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.580 CPU sec, 4.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13349, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 128, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (141.79, 58.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.54, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.396 CPU sec, 4.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13236, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.44, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.68, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.552 CPU sec, 4.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13577, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 139, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (128.29, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.24, 85.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.510 CPU sec, 5.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13681, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 133, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (182.47, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.75, 87.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.846 CPU sec, 4.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13509, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 104, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (139.54, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (183.92, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.254 CPU sec, 4.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13491, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (127.40, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.59, 81.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.595 CPU sec, 7.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13507, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 138, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (134.32, 83.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (177.47, 81.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.548 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13398, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 125, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (124.35, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (176.11, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.544 CPU sec, 4.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13578, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 175, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (180.80, 112.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.33, 83.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.061 CPU sec, 4.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13662, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 132, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (131.72, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (180.65, 84.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.970 CPU sec, 5.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13709, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 152, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (170.62, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.33, 87.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.478 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13699, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 116, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (137.67, 75.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (179.80, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.662 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13378, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 76, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (111.57, 85.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (177.28, 81.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.088 CPU sec, 4.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13421, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 131, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (115.00, 52.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 155, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (174.20, 78.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.321 CPU sec, 4.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13515, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 105, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (134.08, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (175.46, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.944 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13518, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 145, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (193.42, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (179.31, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.253 CPU sec, 5.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13650, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (130.88, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (176.82, 81.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.962 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13801, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (126.61, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.07, 81.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.996 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13703, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 202, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (179.85, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.72, 81.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.816 CPU sec, 4.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13600, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 148, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (184.17, 141.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.21, 83.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.958 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13486, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 122, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (149.79, 95.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 154, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (175.08, 80.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.037 CPU sec, 4.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13743, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 140, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (121.76, 57.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.57, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.740 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13529, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 102, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (98.62, 44.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.17, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.413 CPU sec, 4.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13709, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 143, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (158.67, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (177.72, 81.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.010 CPU sec, 4.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13650, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.97, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.482 CPU sec, 4.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13495, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 125, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (139.25, 80.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (175.83, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.821 CPU sec, 5.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13361, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (176.65, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.302 CPU sec, 4.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13483, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (96.55, 65.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (177.23, 81.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.626 CPU sec, 4.674 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13619, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 151, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (166.00, 100.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.90, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.716 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13724, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 90, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (108.44, 67.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.93, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.279 CPU sec, 4.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13605, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 110, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (133.12, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.74, 82.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.668 CPU sec, 3.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13667, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (152.50, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 155, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.18, 81.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.870 CPU sec, 3.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13607, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (155.33, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 154, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (174.93, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.529 CPU sec, 5.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13611, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 130, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (121.50, 43.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (176.46, 81.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.887 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13560, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (127.06, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (180.16, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.679 CPU sec, 4.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13542, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 97, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (114.60, 51.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.89, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.051 CPU sec, 4.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13498, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (114.71, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (179.05, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.851 CPU sec, 4.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13481, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (119.88, 62.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.64, 81.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.660 CPU sec, 4.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13386, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 152, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (155.69, 63.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (178.01, 80.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.589 CPU sec, 4.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13514, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 155, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (154.05, 101.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (177.78, 82.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.378 CPU sec, 4.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13561, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 149, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (192.68, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.75, 82.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.054 CPU sec, 4.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13569, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 101, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (122.88, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.22, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.334 CPU sec, 4.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13629, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 115, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (119.82, 50.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (180.18, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.193 CPU sec, 4.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13460, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (102.53, 48.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (175.64, 78.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.373 CPU sec, 4.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13374, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 155, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (174.06, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.735 CPU sec, 5.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12975, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (128.79, 78.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 156, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (174.96, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.741 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13491, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 257, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (201.43, 87.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (180.99, 84.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.012 CPU sec, 4.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13784, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 95, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (121.22, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.62, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.180 CPU sec, 4.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13623, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 104, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (106.59, 49.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.34, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.943 CPU sec, 4.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13741, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 131, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (150.78, 86.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (179.75, 83.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.765 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13561, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 131, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (144.50, 70.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.29, 84.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.562 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13620, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 111, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (154.60, 123.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (183.27, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.272 CPU sec, 4.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13556, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 116, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (133.14, 63.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.40, 87.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.358 CPU sec, 4.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13511, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 98, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (114.69, 53.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (186.08, 89.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.696 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13542, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (120.37, 64.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.40, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.300 CPU sec, 5.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13580, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 201, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (184.62, 100.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (175.96, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.192 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13256, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 81, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (109.29, 75.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.31, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.192 CPU sec, 4.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12924, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 113, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (142.72, 81.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (174.09, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.077 CPU sec, 6.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13428, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 103, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (122.07, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (181.57, 85.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.219 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13656, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 106, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (129.74, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.01, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.622 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13687, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 94, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (88.85, 42.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.10, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.700 CPU sec, 4.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13756, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (129.06, 86.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.92, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.207 CPU sec, 4.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13556, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 101, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (148.89, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.54, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.893 CPU sec, 4.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13726, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 125, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (156.38, 94.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.05, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.694 CPU sec, 4.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13692, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 194, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (194.56, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.65, 84.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.471 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13606, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 106, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (132.76, 103.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.57, 86.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.486 CPU sec, 4.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13573, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 127, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 259) [M::mem_pestat] mean and std.dev: (122.46, 48.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (183.79, 86.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.410 CPU sec, 4.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13567, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 104, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (124.05, 72.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (175.91, 79.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.266 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13511, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 113, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (126.67, 99.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (178.81, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.651 CPU sec, 4.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13414, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (137.64, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 156, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (174.85, 78.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.350 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13091, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 103, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (107.71, 52.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.35, 80.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.008 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13836, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 104, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (135.17, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.35, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.883 CPU sec, 5.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13659, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.06, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 113.882 CPU sec, 4.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13626, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 107, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (141.80, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (182.82, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.106 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13537, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (114.90, 50.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.33, 85.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.200 CPU sec, 5.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13541, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 181, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (169.12, 111.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.40, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.328 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13708, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 145, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (152.80, 84.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (182.88, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.942 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13488, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (118.73, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (180.55, 84.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.144 CPU sec, 4.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13776, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (105.46, 64.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.70, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.332 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13602, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (120.54, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (177.85, 81.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.880 CPU sec, 5.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13461, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 135, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 246) [M::mem_pestat] mean and std.dev: (127.79, 32.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (176.32, 79.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.401 CPU sec, 4.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13299, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 103, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (97.00, 67.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (172.06, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.927 CPU sec, 5.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13198, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 88, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (121.55, 97.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 153, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.68, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.025 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13483, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 127, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (114.50, 53.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (177.19, 81.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.112 CPU sec, 4.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13641, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 106, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (117.75, 42.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (178.61, 83.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.117 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13558, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 93, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (127.83, 105.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.08, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.031 CPU sec, 4.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13454, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (167.82, 99.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.17, 81.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.140 CPU sec, 4.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13413, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (131.42, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (178.00, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.855 CPU sec, 5.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13527, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 124, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (141.76, 63.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (181.67, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.403 CPU sec, 4.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13595, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 130, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (135.00, 60.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.26, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.596 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13620, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 209, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (195.50, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.35, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.864 CPU sec, 5.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13658, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (129.33, 59.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (179.83, 84.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.037 CPU sec, 4.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13550, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 98, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (126.82, 89.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (176.92, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.880 CPU sec, 4.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13457, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 114, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (112.33, 50.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 154, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (172.07, 76.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.153 CPU sec, 4.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13079, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 139, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (163.45, 76.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 153, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.71, 72.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.419 CPU sec, 4.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13204, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 117, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 213) [M::mem_pestat] mean and std.dev: (107.71, 31.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (175.43, 79.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.590 CPU sec, 4.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13597, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 138, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (133.80, 59.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (180.16, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.042 CPU sec, 3.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13686, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 94, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (121.87, 61.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (177.13, 81.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.948 CPU sec, 4.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13652, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (177.89, 81.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.975 CPU sec, 4.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13772, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 113, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 216) [M::mem_pestat] mean and std.dev: (111.40, 22.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.39, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.043 CPU sec, 4.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13691, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 156, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (179.15, 79.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (183.99, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.689 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13558, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 82, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (81.19, 44.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (178.65, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.918 CPU sec, 4.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13417, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.32, 67.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (180.84, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.056 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13769, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 168, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (177.19, 108.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.60, 83.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.327 CPU sec, 4.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13567, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 74, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (90.91, 49.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (178.82, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.902 CPU sec, 5.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13459, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 129, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (130.93, 87.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (177.74, 81.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.843 CPU sec, 4.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13430, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 83, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (102.90, 72.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (174.30, 78.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.576 CPU sec, 4.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13142, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 136, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (160.50, 85.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (173.57, 77.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.201 CPU sec, 4.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13632, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 134, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (133.77, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (180.35, 85.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.009 CPU sec, 4.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13665, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 112, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (125.14, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (182.29, 85.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.019 CPU sec, 4.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13680, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 111, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (149.07, 102.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (179.71, 83.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.519 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13662, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 123, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (142.53, 78.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (179.02, 83.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.949 CPU sec, 5.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13617, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 119, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (146.77, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (181.09, 85.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.303 CPU sec, 4.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13703, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (140.22, 86.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (180.44, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.877 CPU sec, 4.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13753, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 164, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (137.22, 45.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (180.47, 85.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.304 CPU sec, 4.709 real sec [M::process] read 31982 sequences (4829282 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13592, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (151.33, 81.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (181.13, 84.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.216 CPU sec, 5.008 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 6490, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (181.88, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 31982 reads in 60.444 CPU sec, 2.400 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21171\tSM:UG10S2P248\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21171_HLKNYBGXX_UG10S2P248_R1.fastq.gz 5347_7332_21171_HLKNYBGXX_UG10S2P248_R2.fastq.gz [main] Real time: 1422.636 sec; CPU: 36944.184 sec Finished alignement of UG10S2P248 at Wed Apr 3 10:20:28 2019 Sorting BAM file started at Wed Apr 3 10:20:28 2019 samtools sort -@ 30 -m 4G UG10S2P248_unsorted.bam -o UG10S2P248.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 10:20:28 2019 rm UG10S2P248_unsorted.bam rm 5347_7332_21171_HLKNYBGXX_UG10S2P248_R1.fastq.gz rm 5347_7332_21171_HLKNYBGXX_UG10S2P248_R2.fastq.gz samtools index UG10S2P248.bam Finished BAM file generation of the sample - UG10S2P248 Processing fastq files for the sample - UG10S1P038 Reading 5347_7332_21172_HLKNYBGXX_UG10S1P038_R1.fastq.gz 5347_7332_21172_HLKNYBGXX_UG10S1P038_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21172\tSM:UG10S1P038\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21172_HLKNYBGXX_UG10S1P038_R1.fastq.gz 5347_7332_21172_HLKNYBGXX_UG10S1P038_R2.fastq.gz | samtools view -Sb1 - > UG10S1P038_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12669, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.01, 66.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.446 CPU sec, 6.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12674, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 179, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (146.33, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (150.88, 67.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.414 CPU sec, 6.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12593, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 111, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (128.00, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.72, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.323 CPU sec, 6.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12680, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 102, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (114.12, 65.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (150.23, 66.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.770 CPU sec, 6.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12852, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 128, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (128.64, 63.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (148.50, 65.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.598 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12671, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (128.40, 99.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (148.17, 65.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.078 CPU sec, 5.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12607, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 111, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (119.65, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (147.01, 63.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.717 CPU sec, 6.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12567, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 70, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (102.64, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (150.61, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.571 CPU sec, 6.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12821, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (151.25, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.576 CPU sec, 6.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12671, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (135.81, 99.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (151.12, 68.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.668 CPU sec, 6.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12857, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (149.25, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.05, 66.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.396 CPU sec, 6.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12761, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 78, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (146.89, 163.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (150.41, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.907 CPU sec, 6.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12715, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (101.50, 60.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.27, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.313 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12527, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (134.70, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.98, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.172 CPU sec, 6.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12671, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (30, 123, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (114.67, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (148.92, 66.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.616 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12650, 6, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.94, 65.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.974 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12740, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (107.06, 59.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.53, 66.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.589 CPU sec, 6.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12720, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (136.92, 83.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (147.51, 65.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.345 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12705, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 88, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (99.83, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.25, 64.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.156 CPU sec, 6.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12747, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 78, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (91.44, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (147.31, 65.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.302 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12722, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 102, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (108.92, 56.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.21, 65.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.244 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12629, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 112, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (149.08, 111.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (149.03, 65.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.076 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12807, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (146.31, 63.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.749 CPU sec, 6.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12716, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (147.05, 65.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.963 CPU sec, 5.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12736, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 87, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (93.36, 53.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.30, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.664 CPU sec, 5.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12647, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.86, 65.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.674 CPU sec, 6.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12672, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 79, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (107.92, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.26, 65.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.299 CPU sec, 6.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12743, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (148.21, 64.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.776 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12639, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.82, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.695 CPU sec, 7.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12660, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (148.58, 65.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.174 CPU sec, 6.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12782, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.47, 65.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.769 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12710, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 84, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (101.80, 61.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (147.11, 63.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.169 CPU sec, 5.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12508, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 160, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (138.80, 63.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (146.29, 64.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.792 CPU sec, 5.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12789, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 152, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (162.69, 104.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.21, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.964 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12616, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 93, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (92.27, 52.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.95, 66.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.531 CPU sec, 6.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12719, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 85, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (85.69, 54.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (147.91, 64.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.648 CPU sec, 6.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12486, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 79, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (128.45, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.76, 64.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.432 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12595, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 137, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (132.07, 56.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.44, 62.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.192 CPU sec, 6.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12741, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 116, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (129.50, 71.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.69, 64.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.140 CPU sec, 6.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12747, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 86, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (107.64, 64.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (150.34, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.011 CPU sec, 5.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12741, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 142, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (143.53, 104.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (150.85, 67.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.489 CPU sec, 5.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12637, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (110.06, 55.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (150.89, 67.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.164 CPU sec, 6.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12701, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 107, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (140.15, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.32, 64.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.134 CPU sec, 6.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12669, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 63, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (94.80, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (147.37, 64.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.591 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12117, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.28, 63.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 184.285 CPU sec, 6.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12616, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (126.67, 64.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (149.35, 67.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.016 CPU sec, 5.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12764, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 67, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (71.25, 32.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (150.14, 66.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.730 CPU sec, 6.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12571, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (140.38, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.22, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.126 CPU sec, 6.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12617, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 112, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (120.91, 52.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (149.00, 65.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.638 CPU sec, 6.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12845, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 90, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (85.45, 41.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.25, 62.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.954 CPU sec, 6.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12623, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (32, 65, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (86.36, 70.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (145.78, 62.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.977 CPU sec, 5.609 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12382, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (124.67, 78.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (150.12, 66.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.090 CPU sec, 5.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12676, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 88, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (93.67, 57.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (151.83, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.785 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12610, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 83, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (93.77, 34.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (149.96, 66.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.889 CPU sec, 6.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12601, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (148.07, 65.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.132 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12612, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 103, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (86.50, 21.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.82, 66.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.300 CPU sec, 6.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12624, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (128.91, 69.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.55, 63.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.076 CPU sec, 6.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12496, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 145, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (114.00, 65.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (145.93, 63.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.455 CPU sec, 6.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12667, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 73, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (82.62, 47.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (150.88, 68.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.863 CPU sec, 6.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12504, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (151.04, 67.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.912 CPU sec, 6.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12709, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 87, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (87.57, 52.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.64, 64.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.916 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12674, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (147.51, 63.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.819 CPU sec, 6.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12729, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 192, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (174.33, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.79, 63.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.295 CPU sec, 5.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12322, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.60, 62.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.842 CPU sec, 6.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12554, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (151.28, 68.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.427 CPU sec, 6.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12588, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 134, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (144.60, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (149.71, 67.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.446 CPU sec, 6.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12690, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 103, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (112.64, 63.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (149.53, 66.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.927 CPU sec, 6.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12784, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 69, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (74.73, 43.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (151.78, 68.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.884 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12582, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 97, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (89.30, 44.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (145.95, 62.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.276 CPU sec, 6.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12632, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 134, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (123.90, 77.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.72, 62.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.809 CPU sec, 5.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12445, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (127.22, 76.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.29, 66.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.038 CPU sec, 5.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12684, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 99, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (92.11, 34.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (151.89, 68.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.000 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12732, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 145, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (147.62, 76.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (151.58, 68.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.529 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12704, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 126, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (178.13, 115.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (150.23, 67.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.798 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12709, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (135.70, 91.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (148.03, 64.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.277 CPU sec, 6.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12583, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 74, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (82.42, 52.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.64, 65.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.989 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12477, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 142, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (128.44, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (144.35, 60.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.731 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12771, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 101, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (129.93, 94.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (148.29, 65.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.892 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12831, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 166, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (119.80, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.04, 65.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.000 CPU sec, 6.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12721, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 129, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (126.40, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.21, 65.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.685 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12701, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 157, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (133.22, 59.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (146.89, 64.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.560 CPU sec, 6.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12694, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 130, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (136.72, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.98, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.677 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12796, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 80, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (126.92, 95.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (148.68, 65.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.065 CPU sec, 7.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12662, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 118, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (119.17, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (149.32, 66.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.914 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12526, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 88, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (92.08, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (147.14, 63.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.236 CPU sec, 6.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12707, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (148.36, 109.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.97, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.892 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12832, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 79, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (70.82, 16.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.06, 64.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.681 CPU sec, 6.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12503, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 90, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (122.18, 70.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.36, 64.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.706 CPU sec, 7.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12700, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.13, 64.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.744 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12686, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (145.64, 62.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.687 CPU sec, 5.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12923, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 74, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (70.91, 30.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (146.86, 63.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.217 CPU sec, 6.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12682, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.42, 65.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.929 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12674, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 70, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (66.56, 24.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.67, 64.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.947 CPU sec, 6.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12615, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 103, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 206) [M::mem_pestat] mean and std.dev: (109.42, 30.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (149.46, 67.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.866 CPU sec, 6.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12923, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 115, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (183.25, 153.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (150.04, 67.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.612 CPU sec, 6.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12644, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 174, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (134.21, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (148.27, 64.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.846 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12639, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (149.12, 65.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.476 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12646, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (147.84, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.47, 65.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.872 CPU sec, 7.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12639, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 112, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (125.41, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (148.09, 66.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.783 CPU sec, 6.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12487, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 108, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (115.10, 73.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (146.28, 64.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.212 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12741, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 108, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (143.83, 122.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (149.31, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.058 CPU sec, 5.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12739, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 78, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (96.00, 48.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (148.71, 65.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.530 CPU sec, 6.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12735, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (127.10, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (147.99, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.332 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12640, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (102.78, 24.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.83, 64.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.130 CPU sec, 5.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12762, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (148.89, 65.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 179.593 CPU sec, 6.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12659, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 128, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (159.83, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (146.63, 63.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.369 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12607, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 150, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (181.75, 142.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.63, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.303 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12889, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 72, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (117.53, 76.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.81, 65.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.366 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12751, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 74, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (103.00, 71.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.08, 64.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.182 CPU sec, 5.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12728, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 140, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (108.10, 78.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (146.43, 63.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.400 CPU sec, 6.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12693, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 75, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (76.87, 26.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.59, 63.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.260 CPU sec, 6.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12786, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (147.36, 63.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.133 CPU sec, 5.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12728, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 103, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (113.31, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (147.64, 64.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.079 CPU sec, 6.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12865, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 136, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (134.00, 44.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (149.10, 65.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.344 CPU sec, 6.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12705, 1, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.13, 64.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.573 CPU sec, 6.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12591, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (144.28, 61.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.441 CPU sec, 6.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12669, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 78, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (82.31, 34.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (150.19, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.041 CPU sec, 6.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12785, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 119, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (130.93, 66.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.50, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.159 CPU sec, 5.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12752, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (125.73, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (147.97, 64.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.988 CPU sec, 6.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12889, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 93, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (136.69, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (150.90, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.848 CPU sec, 6.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12754, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (90.55, 36.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (146.79, 64.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.627 CPU sec, 6.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12639, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (145.92, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.671 CPU sec, 6.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12710, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 120, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (120.08, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (150.19, 67.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.431 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12490, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 149, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (154.39, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (149.07, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.716 CPU sec, 6.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12526, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 141, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (133.50, 87.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (151.24, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.176 CPU sec, 6.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12801, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (151.32, 68.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.557 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12862, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (140.00, 83.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (147.93, 65.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.080 CPU sec, 6.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12581, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (153.60, 102.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (149.19, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.328 CPU sec, 5.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12528, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 125, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (127.54, 45.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.88, 63.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.877 CPU sec, 6.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12740, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 126, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (132.50, 61.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (148.28, 65.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.574 CPU sec, 5.609 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12699, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 90, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (113.83, 70.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (148.95, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.555 CPU sec, 5.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12876, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 124, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (111.23, 46.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (151.36, 67.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.164 CPU sec, 5.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12779, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (65.90, 31.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (149.02, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.809 CPU sec, 5.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12647, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 102, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (108.15, 54.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.08, 62.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.244 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12381, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 85, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (76.08, 22.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.46, 62.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.311 CPU sec, 6.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12707, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (128.29, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (146.10, 63.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.548 CPU sec, 6.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12708, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 249, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (167.50, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (148.10, 64.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.488 CPU sec, 6.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12640, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 124, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (132.77, 63.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (148.08, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.793 CPU sec, 6.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12552, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 98, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (113.50, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.81, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.144 CPU sec, 6.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12628, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (25, 58, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (92.92, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (145.04, 61.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.899 CPU sec, 6.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12487, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.78, 60.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.001 CPU sec, 6.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12724, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 112, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (151.73, 76.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.87, 65.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.888 CPU sec, 6.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12803, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 60, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (85.00, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (149.45, 66.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.495 CPU sec, 6.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12635, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.29, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.844 CPU sec, 6.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12662, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 187) [M::mem_pestat] mean and std.dev: (98.25, 25.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.15, 64.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.034 CPU sec, 5.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12737, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (146.89, 63.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.860 CPU sec, 6.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12698, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.83, 61.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.602 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12663, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (121.79, 70.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.35, 64.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.215 CPU sec, 6.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12800, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (99.36, 48.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (147.96, 64.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.367 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12904, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 95, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (108.56, 63.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (149.46, 66.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.727 CPU sec, 7.106 real sec [M::process] read 5962 sequences (900262 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12812, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 101, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (92.92, 28.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.08, 64.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.668 CPU sec, 6.411 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 1136, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (150.69, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 5962 reads in 15.824 CPU sec, 0.698 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21172\tSM:UG10S1P038\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21172_HLKNYBGXX_UG10S1P038_R1.fastq.gz 5347_7332_21172_HLKNYBGXX_UG10S1P038_R2.fastq.gz [main] Real time: 936.176 sec; CPU: 25668.242 sec Finished alignement of UG10S1P038 at Wed Apr 3 10:45:51 2019 Sorting BAM file started at Wed Apr 3 10:45:51 2019 samtools sort -@ 30 -m 4G UG10S1P038_unsorted.bam -o UG10S1P038.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 10:45:51 2019 rm UG10S1P038_unsorted.bam rm 5347_7332_21172_HLKNYBGXX_UG10S1P038_R1.fastq.gz rm 5347_7332_21172_HLKNYBGXX_UG10S1P038_R2.fastq.gz samtools index UG10S1P038.bam Finished BAM file generation of the sample - UG10S1P038 Processing fastq files for the sample - UG10S2P412 Reading 5347_7332_21173_HLKNYBGXX_UG10S2P412_R1.fastq.gz 5347_7332_21173_HLKNYBGXX_UG10S2P412_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21173\tSM:UG10S2P412\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21173_HLKNYBGXX_UG10S2P412_R1.fastq.gz 5347_7332_21173_HLKNYBGXX_UG10S2P412_R2.fastq.gz | samtools view -Sb1 - > UG10S2P412_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12801, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (156.12, 92.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.17, 121.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.990 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12952, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (182.20, 62.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.08, 121.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.247 CPU sec, 4.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13032, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (169.06, 87.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.13, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.689 CPU sec, 4.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13095, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 176, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (207.06, 111.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (239.54, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.233 CPU sec, 4.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13028, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 246, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (197.80, 111.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (243.35, 124.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.262 CPU sec, 4.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13232, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 140, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (170.09, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (243.60, 125.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.492 CPU sec, 5.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13186, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 149, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (194.21, 140.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (243.49, 125.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.350 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13125, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 129, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (168.79, 161.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (241.97, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.314 CPU sec, 4.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13179, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 137, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (145.77, 105.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.27, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.245 CPU sec, 4.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13200, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (144.93, 89.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.01, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.126 CPU sec, 4.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13022, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 243, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (229.00, 143.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.76, 123.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.660 CPU sec, 5.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13043, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 153, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (127.62, 70.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.13, 120.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.204 CPU sec, 4.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13167, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 200, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (189.22, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.87, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.276 CPU sec, 4.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13232, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (117.91, 75.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.63, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.803 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12926, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 162, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (175.72, 120.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (233.87, 118.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.624 CPU sec, 5.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13023, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 192, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (188.00, 110.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.05, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.396 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12578, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 136, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (146.33, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (241.96, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.829 CPU sec, 5.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12755, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (189.08, 110.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.10, 121.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.219 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12876, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 123, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (154.00, 88.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.33, 120.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.844 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13145, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 148, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (141.64, 67.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.97, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.915 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13096, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 113, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (156.38, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.83, 122.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.914 CPU sec, 4.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13167, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (134.33, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.56, 123.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.678 CPU sec, 5.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13206, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 130, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (142.00, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.14, 122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.334 CPU sec, 4.543 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13200, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 122, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (117.65, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.50, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.412 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13158, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 240, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (224.00, 143.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.11, 121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.061 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13076, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.11, 120.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.887 CPU sec, 4.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13177, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 287, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (252.15, 150.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.33, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.562 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13122, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (177.38, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (236.58, 120.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.177 CPU sec, 5.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13199, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 96, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (145.68, 99.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.01, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.026 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12956, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 145, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (136.92, 54.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.40, 119.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.257 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12986, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 179, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (191.35, 106.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (233.97, 119.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.339 CPU sec, 5.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12977, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 116, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (117.00, 49.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.34, 117.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.592 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12794, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (174.50, 84.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.82, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.457 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13001, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 239, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (202.81, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.13, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.557 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13039, 3, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.58, 119.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.267 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13207, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 130, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (142.62, 91.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.14, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.598 CPU sec, 4.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12869, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.22, 122.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.402 CPU sec, 4.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13025, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 145, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (160.45, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (240.77, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.814 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12976, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 232, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (238.00, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.79, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.523 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13160, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 98, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (159.43, 116.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.45, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.036 CPU sec, 4.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13000, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 155, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (190.69, 142.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (237.95, 122.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.911 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13027, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 92, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.19, 78.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.05, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.012 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12970, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 171, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (230.46, 169.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.63, 120.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.329 CPU sec, 6.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13130, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 206, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (222.44, 148.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.70, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.531 CPU sec, 4.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13047, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 171, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (212.08, 153.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (232.82, 117.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.399 CPU sec, 4.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12912, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 317) [M::mem_pestat] mean and std.dev: (155.85, 59.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.84, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.894 CPU sec, 5.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12913, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 146, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (177.30, 105.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (233.06, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.951 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12966, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 154, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (169.62, 105.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.23, 118.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.197 CPU sec, 4.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12825, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 161, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (198.06, 171.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.43, 116.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.348 CPU sec, 4.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12815, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 157, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (158.56, 99.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (233.50, 118.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.066 CPU sec, 4.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12960, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 163, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (146.50, 82.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.28, 119.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.926 CPU sec, 5.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13167, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 211, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (198.00, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.43, 121.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.186 CPU sec, 4.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13030, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 146, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (125.38, 74.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.67, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.930 CPU sec, 5.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13088, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 216, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (202.00, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.58, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.335 CPU sec, 5.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13081, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (130.36, 62.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.92, 121.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.089 CPU sec, 4.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12954, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 177, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (161.46, 83.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (238.63, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.538 CPU sec, 4.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13088, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 194, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (199.68, 112.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.46, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.861 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13065, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (134.00, 71.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.15, 121.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.904 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13111, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 130, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (141.44, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.52, 119.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.177 CPU sec, 4.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12980, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 122, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (177.67, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.46, 115.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.060 CPU sec, 4.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12844, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (143.21, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.30, 116.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.740 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12996, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 191, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (177.53, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.19, 117.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.206 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12905, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 162, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (161.43, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.81, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.271 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12935, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 167, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (186.31, 115.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (231.43, 115.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.267 CPU sec, 4.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12899, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 206, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (233.50, 144.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.21, 115.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.942 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12790, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 177, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (189.29, 128.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (232.56, 117.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.381 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12891, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 136, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (149.00, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.68, 119.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.544 CPU sec, 4.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12938, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 104, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (142.00, 124.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.15, 119.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.138 CPU sec, 6.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13123, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 137, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (167.70, 119.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.49, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.843 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13048, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 185, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (187.29, 107.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.29, 121.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.706 CPU sec, 4.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13060, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 219, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (203.00, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.70, 120.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.497 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13290, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 175, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (206.78, 102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.74, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.367 CPU sec, 4.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12944, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (234.97, 120.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.474 CPU sec, 3.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13210, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 197, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (196.27, 112.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.44, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.706 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12964, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 94, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (115.82, 62.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.62, 121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.574 CPU sec, 4.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13117, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 140, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (185.57, 134.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.55, 119.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.371 CPU sec, 4.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13018, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (180.16, 116.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.69, 116.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.250 CPU sec, 5.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13082, 8, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 158, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (154.57, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.14, 118.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.164 CPU sec, 5.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12952, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 182, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (139.92, 66.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (231.83, 115.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.088 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13103, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 132, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (165.06, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.76, 116.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.123 CPU sec, 4.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12935, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 129, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.55, 53.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (233.12, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.433 CPU sec, 5.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12793, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (158.24, 104.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (232.91, 116.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.801 CPU sec, 6.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12818, 6, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (234.68, 119.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.329 CPU sec, 4.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12662, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (149.88, 107.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.74, 118.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.446 CPU sec, 4.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12911, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 163, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (202.27, 110.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (233.29, 117.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.550 CPU sec, 5.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13052, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 129, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (134.58, 84.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.61, 119.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.882 CPU sec, 6.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13100, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (189.33, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.24, 121.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.424 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13088, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 131, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (133.35, 72.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.56, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.210 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12996, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 217, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (192.71, 101.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.99, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.527 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13086, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 139, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (166.64, 103.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.14, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.541 CPU sec, 4.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12858, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.59, 117.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.360 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13039, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 135, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (173.18, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.85, 117.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.645 CPU sec, 4.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12946, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (190.79, 136.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (229.70, 115.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.417 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12836, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 153, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (132.11, 71.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.35, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.133 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13012, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 241, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (213.84, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (230.19, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.034 CPU sec, 5.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12809, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 151, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (157.29, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (229.89, 115.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.006 CPU sec, 4.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13055, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 94, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (111.30, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.26, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.659 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13053, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 165, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (183.56, 100.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (241.43, 122.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.783 CPU sec, 4.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13064, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 191, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (170.36, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (242.13, 123.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.351 CPU sec, 4.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13230, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 159, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (240.09, 232.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.49, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.971 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13031, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 112, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (123.67, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.33, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.309 CPU sec, 4.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13011, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 187, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (194.89, 109.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (240.52, 123.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.736 CPU sec, 4.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13228, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 121, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (143.54, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.07, 122.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.709 CPU sec, 4.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13065, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 98, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (139.83, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (240.21, 122.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.609 CPU sec, 3.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13121, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 280, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (211.91, 108.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.80, 120.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.623 CPU sec, 4.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13017, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 126, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (161.95, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.73, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.924 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13035, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 129, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (171.12, 100.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.91, 119.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.828 CPU sec, 4.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13096, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 190, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (231.07, 199.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.60, 119.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.148 CPU sec, 3.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12957, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 108, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (130.27, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.93, 118.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.953 CPU sec, 4.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12795, 8, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 189, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (229.59, 178.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.67, 117.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.129 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12661, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 133, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (146.29, 58.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (230.67, 116.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.315 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12376, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 137, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (191.68, 121.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (226.99, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.237 CPU sec, 5.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12495, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (170.07, 108.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (233.18, 118.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.383 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12896, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (243.37, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.000 CPU sec, 4.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13132, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 130, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (159.21, 113.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (242.28, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.291 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13036, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 161, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (139.59, 66.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (243.08, 124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.481 CPU sec, 5.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13023, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (136.73, 93.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (241.57, 124.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.264 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13077, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 158, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (174.59, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 210, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (245.01, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.605 CPU sec, 4.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13030, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 133, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (138.60, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 209, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (241.59, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.886 CPU sec, 5.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13094, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (169.83, 80.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.28, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.754 CPU sec, 4.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13082, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.94, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.446 CPU sec, 4.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13020, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 159, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (171.06, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (240.66, 123.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.751 CPU sec, 5.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12902, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 128, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (162.40, 86.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.39, 121.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.588 CPU sec, 4.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13151, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 206, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (175.73, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.71, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.643 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12987, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 242, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (255.93, 177.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (233.65, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.353 CPU sec, 4.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12832, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 171, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (151.79, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (231.70, 116.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.448 CPU sec, 4.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12649, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 167, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (204.38, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.73, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.290 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12636, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 152, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (176.13, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (229.93, 115.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.693 CPU sec, 4.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12222, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 88, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (94.90, 40.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.55, 117.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.298 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12838, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 165, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (174.94, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (245.74, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.616 CPU sec, 5.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12989, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 172, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (186.50, 119.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (244.96, 125.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.893 CPU sec, 4.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13068, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 133, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (134.73, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (243.34, 123.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.242 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13028, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (178.84, 104.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (242.74, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.198 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13000, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 248, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (221.94, 139.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.90, 122.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.429 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13082, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 124, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (139.59, 92.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.05, 121.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.549 CPU sec, 4.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13080, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 132, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (221.00, 129.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.52, 122.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.669 CPU sec, 4.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13006, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 157, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (161.82, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.98, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.611 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13046, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 145, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (162.25, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (240.99, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.653 CPU sec, 5.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13018, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 138, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (142.62, 67.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.25, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.740 CPU sec, 4.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13194, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 198, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (201.00, 113.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.55, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.389 CPU sec, 4.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12998, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 190, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (207.00, 101.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.77, 117.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.484 CPU sec, 5.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12831, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.63, 115.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.071 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12798, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 121, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (152.06, 127.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (226.86, 113.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.082 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12550, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (138.12, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (227.00, 113.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.369 CPU sec, 4.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12382, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (112.27, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (230.75, 115.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.841 CPU sec, 5.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13005, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (113.83, 63.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.18, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.495 CPU sec, 5.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13002, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (238.68, 120.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.541 CPU sec, 4.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12904, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 226, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (234.00, 126.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (237.21, 118.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.900 CPU sec, 5.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13051, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.11, 120.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 129.612 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12946, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (174.47, 78.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.78, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.104 CPU sec, 4.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13035, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 180, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (158.08, 72.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.02, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.661 CPU sec, 5.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13175, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 169, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (149.38, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.78, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.739 CPU sec, 5.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13014, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 164, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (170.63, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.92, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.732 CPU sec, 4.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13181, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (210.60, 108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.52, 120.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.937 CPU sec, 4.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13056, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 144, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.33, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.39, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.073 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12893, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 129, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (143.04, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.76, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.013 CPU sec, 4.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12948, 11, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 149, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (235.93, 196.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.32, 116.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 4166, 5379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14913) [M::mem_pestat] mean and std.dev: (3599.55, 2865.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19680) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 116.935 CPU sec, 4.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12858, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 96, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (121.86, 71.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (224.45, 111.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.293 CPU sec, 4.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12833, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 96, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (147.92, 111.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (228.44, 113.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.111 CPU sec, 4.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12549, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 240, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (208.23, 95.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (227.61, 112.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.785 CPU sec, 5.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12472, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 155, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (176.17, 112.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (226.01, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.136 CPU sec, 6.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12954, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 231, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (249.53, 114.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.77, 124.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.809 CPU sec, 6.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12904, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 102, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (91.25, 43.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.69, 121.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.940 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12958, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 212, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (185.31, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (240.67, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.962 CPU sec, 4.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13018, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 134, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (171.92, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.41, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.595 CPU sec, 4.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13120, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (201.10, 116.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (240.07, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.743 CPU sec, 4.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13095, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (106.64, 50.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (239.98, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.901 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13037, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 284, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (224.43, 131.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.42, 122.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.325 CPU sec, 4.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13058, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 144, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (151.65, 74.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (239.99, 121.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.200 CPU sec, 4.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13114, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (148.67, 113.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.82, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.616 CPU sec, 4.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12926, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 135, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (165.38, 106.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (236.17, 119.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.207 CPU sec, 5.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13140, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 166, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (187.07, 110.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.42, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.814 CPU sec, 4.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12972, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 153, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (181.88, 129.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (226.86, 113.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.254 CPU sec, 5.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12855, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 135, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (151.44, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (228.51, 114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.035 CPU sec, 4.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12860, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 183, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (161.50, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (226.35, 112.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.767 CPU sec, 3.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12366, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 115, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (120.12, 61.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (224.57, 110.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.736 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12267, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 122, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (146.83, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (225.23, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.274 CPU sec, 4.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12998, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 182, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (164.12, 77.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (241.67, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.297 CPU sec, 5.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13074, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (100.29, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (240.00, 122.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.873 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12987, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (123.44, 71.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.10, 122.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.948 CPU sec, 5.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13048, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 145, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (135.56, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.44, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.637 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13030, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 146, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (118.30, 33.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.20, 121.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.271 CPU sec, 4.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12880, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 82, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (102.36, 61.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.08, 121.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.898 CPU sec, 5.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13163, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 122, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (110.91, 55.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.19, 120.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.838 CPU sec, 4.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12947, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 162, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (153.31, 81.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.32, 120.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.073 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13120, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 116, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (116.07, 44.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.60, 120.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.689 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13133, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 130, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (156.42, 106.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.56, 120.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.088 CPU sec, 4.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13149, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 178, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (159.00, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (232.87, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.936 CPU sec, 4.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12880, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 132, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (151.61, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (229.66, 116.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.018 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12858, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 233, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (271.95, 168.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (226.94, 111.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.597 CPU sec, 4.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12765, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 150, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (152.80, 81.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 190, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (223.51, 111.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.180 CPU sec, 3.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12410, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 163, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (143.33, 92.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (223.21, 108.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.461 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12385, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 164, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (139.43, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (225.27, 111.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.831 CPU sec, 4.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12779, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 145, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (193.11, 107.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.31, 117.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.364 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13058, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (144.14, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (233.38, 116.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.537 CPU sec, 4.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12833, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 90, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (244.27, 230.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (230.67, 115.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.038 CPU sec, 5.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13060, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 135, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (115.58, 34.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.17, 116.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.777 CPU sec, 4.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12971, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 113, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (172.07, 127.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.78, 119.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.333 CPU sec, 4.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13161, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 170, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (160.24, 108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.61, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.405 CPU sec, 5.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13173, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 311, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (276.83, 147.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (240.46, 123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.579 CPU sec, 4.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13182, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 153, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (152.90, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (239.17, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.429 CPU sec, 4.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13124, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 168, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (202.26, 129.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (234.67, 119.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.244 CPU sec, 4.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13215, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 172, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (168.46, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.39, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.210 CPU sec, 4.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12694, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (159.00, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.83, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.501 CPU sec, 4.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12955, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 261, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (243.91, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.43, 120.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.570 CPU sec, 5.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12966, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 184, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (205.12, 111.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.23, 121.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.092 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12941, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (171.33, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (236.59, 121.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.953 CPU sec, 4.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13122, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (213.83, 116.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.24, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.641 CPU sec, 4.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13043, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 110, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (168.92, 143.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.99, 120.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.543 CPU sec, 4.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12890, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 237, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (153.67, 95.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (234.08, 118.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.284 CPU sec, 4.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13042, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 168, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (181.24, 113.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (233.30, 117.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.138 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13005, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (175.29, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.56, 116.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.520 CPU sec, 5.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12856, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 155, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (153.73, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.98, 116.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.075 CPU sec, 6.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13115, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 177, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (174.27, 108.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.08, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.255 CPU sec, 4.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13043, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 206, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (164.28, 108.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (240.53, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.444 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13035, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (153.76, 111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.66, 120.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.527 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13031, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 156, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (177.92, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.45, 122.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.927 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13125, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 149, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (169.45, 59.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.82, 122.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.661 CPU sec, 4.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13045, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 211, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (210.25, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.79, 120.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.732 CPU sec, 4.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12461, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 103, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (94.80, 38.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.11, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.341 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13010, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 186, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (156.00, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.44, 118.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.991 CPU sec, 4.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12958, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 115, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (120.90, 45.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.98, 119.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.777 CPU sec, 4.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13220, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (144.36, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (234.94, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.630 CPU sec, 4.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13080, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (238.73, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 112.343 CPU sec, 4.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13024, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 227, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (173.09, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.78, 117.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.266 CPU sec, 4.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13100, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 138, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (144.89, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.83, 116.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.402 CPU sec, 6.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13150, 9, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 178, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (220.48, 144.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (229.61, 114.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.762 CPU sec, 4.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12963, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 85, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (115.44, 78.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.26, 115.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.914 CPU sec, 4.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13046, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 158, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (136.87, 66.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (231.82, 118.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.502 CPU sec, 4.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13062, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 171, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (201.90, 115.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.28, 122.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.908 CPU sec, 4.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13123, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 116, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (119.43, 57.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.25, 121.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.927 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13193, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 166, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (159.94, 71.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.79, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.840 CPU sec, 4.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13037, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 194, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (202.38, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.35, 121.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.531 CPU sec, 4.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13099, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.75, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 109.127 CPU sec, 3.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12708, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 148, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (177.08, 103.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (235.48, 118.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.824 CPU sec, 4.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12605, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 92, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (151.45, 122.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.76, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.784 CPU sec, 5.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13108, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 239, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (241.21, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.57, 122.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.127 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13109, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 178, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (184.44, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 207, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (239.76, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.632 CPU sec, 4.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12930, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 133, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (159.35, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (240.19, 122.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.067 CPU sec, 4.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13061, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (131.06, 83.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.24, 118.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.589 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12983, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 145, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (169.00, 116.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.43, 115.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.367 CPU sec, 4.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12958, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (156.00, 99.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (232.69, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.643 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12975, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 133, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (161.33, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.04, 116.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.509 CPU sec, 4.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12766, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 114, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (123.81, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (233.33, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.567 CPU sec, 4.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13189, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 206, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (205.31, 100.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (236.17, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.745 CPU sec, 4.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12937, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 181, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434) [M::mem_pestat] mean and std.dev: (254.45, 233.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.17, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.603 CPU sec, 5.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13163, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.54, 119.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.692 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13050, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.42, 119.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 112.431 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13241, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 179, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (183.94, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.13, 118.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.284 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13020, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 177, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (174.06, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (230.47, 114.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.036 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12608, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (156.56, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (227.70, 112.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.226 CPU sec, 4.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12854, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 227, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (212.85, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.22, 117.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.966 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12946, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 143, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (168.00, 72.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.25, 120.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.684 CPU sec, 4.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12985, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 125, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (128.60, 70.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.09, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.453 CPU sec, 4.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13145, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 166, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (190.29, 130.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.32, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.363 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12952, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (138.00, 77.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.15, 116.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.816 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13065, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 74, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (99.73, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.99, 116.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.146 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12892, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 150, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (144.57, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (229.23, 115.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.799 CPU sec, 4.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12801, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 192, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (219.86, 153.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (229.56, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.479 CPU sec, 4.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12945, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 179, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (180.67, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (230.44, 115.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.670 CPU sec, 4.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13121, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 181, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (195.06, 118.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.94, 118.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.902 CPU sec, 4.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13026, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (192.72, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.01, 118.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.976 CPU sec, 4.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13086, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 120, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (159.93, 101.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.03, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.433 CPU sec, 4.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12999, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 110, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (150.47, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.44, 118.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.700 CPU sec, 4.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13013, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.28, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.230 CPU sec, 5.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12562, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (163.71, 115.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.28, 113.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.486 CPU sec, 4.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12991, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 146, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (183.33, 108.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (227.15, 113.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.955 CPU sec, 3.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12997, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 189, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (177.11, 98.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.00, 117.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.124 CPU sec, 5.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13120, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 134, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (158.33, 104.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.30, 118.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.692 CPU sec, 4.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13011, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 181, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (176.56, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (233.74, 119.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.825 CPU sec, 4.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13082, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 169, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (189.29, 106.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.25, 117.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.706 CPU sec, 4.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12945, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (204.35, 145.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.53, 115.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.352 CPU sec, 3.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12857, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 150, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (177.14, 110.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (228.64, 115.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.471 CPU sec, 5.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12928, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 91, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (129.62, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (228.25, 113.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.963 CPU sec, 4.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12853, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 145, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (156.00, 96.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.12, 112.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.127 CPU sec, 4.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12975, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 181, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (201.20, 103.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.79, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.847 CPU sec, 6.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13185, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 167, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (169.44, 107.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (234.59, 119.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.215 CPU sec, 4.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12986, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (157.82, 101.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.70, 121.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.024 CPU sec, 4.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13009, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 140, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (162.00, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.98, 117.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.780 CPU sec, 4.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13021, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 292, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (223.11, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (231.92, 117.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.418 CPU sec, 5.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12974, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 154, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (150.94, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.52, 116.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.791 CPU sec, 4.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12725, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 107, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (193.90, 203.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.41, 115.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.597 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12911, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.33, 116.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.023 CPU sec, 4.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13073, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 164, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (178.42, 114.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.86, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.234 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13019, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 164, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (185.65, 150.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (234.85, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.051 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13194, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (218.23, 107.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.69, 119.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.368 CPU sec, 4.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12944, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 207, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (199.12, 103.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (228.87, 115.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.346 CPU sec, 3.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12826, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (130.29, 56.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (228.89, 113.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.476 CPU sec, 5.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12630, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 89, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (97.88, 55.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (228.57, 113.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.737 CPU sec, 4.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12405, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (191.17, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (227.39, 112.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.559 CPU sec, 5.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12308, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (141.00, 79.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (228.98, 113.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.476 CPU sec, 5.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13022, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 166, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (151.00, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (236.53, 119.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.420 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13014, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 189, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (167.08, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.02, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.280 CPU sec, 4.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13083, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 86, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (120.93, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.23, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.171 CPU sec, 4.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13153, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 92, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (124.22, 91.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.59, 120.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.727 CPU sec, 4.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13153, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 171, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (177.65, 144.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (233.06, 118.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.754 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12798, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 101, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (136.00, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.19, 119.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.223 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12882, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 122, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (127.71, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.63, 121.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.969 CPU sec, 4.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13151, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 162, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (197.35, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.79, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.408 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13189, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 170, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (188.17, 107.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (238.63, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.844 CPU sec, 4.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13102, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 131, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (151.15, 78.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.77, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.638 CPU sec, 4.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13123, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (194.95, 166.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.53, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.801 CPU sec, 4.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12911, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 147, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (161.21, 116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.33, 115.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.176 CPU sec, 5.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12872, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (228.38, 222.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.70, 115.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.093 CPU sec, 4.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12518, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 162, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (180.23, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (225.90, 112.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.676 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12457, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (201.78, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (227.41, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.649 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12624, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (209.92, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (236.55, 120.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.827 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13149, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 207, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (178.57, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.62, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.675 CPU sec, 5.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13154, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 153, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (129.54, 109.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.59, 122.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.849 CPU sec, 5.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13075, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 94, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (95.00, 42.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (238.13, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.146 CPU sec, 4.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13076, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 143, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (162.77, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.15, 119.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.313 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13089, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 179, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (162.13, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.40, 118.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.917 CPU sec, 4.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13021, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (154.22, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (239.51, 121.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.168 CPU sec, 4.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13152, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 172, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (159.84, 66.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (241.75, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.194 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13159, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (157.75, 106.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (240.84, 123.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.655 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13211, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 227, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (186.50, 89.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.52, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.838 CPU sec, 4.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13208, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 148, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (143.40, 77.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (240.31, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.548 CPU sec, 5.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13008, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 217, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (182.54, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.34, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.978 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12971, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (120.21, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.13, 116.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.897 CPU sec, 5.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12813, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 155, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (148.95, 70.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (227.60, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.739 CPU sec, 4.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12576, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 185, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (208.18, 166.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (229.75, 114.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.089 CPU sec, 4.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12394, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (115.53, 78.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (230.63, 115.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.766 CPU sec, 4.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12936, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (177.81, 117.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.80, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.361 CPU sec, 4.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13031, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 163, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (175.00, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.73, 121.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.031 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13044, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 133, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (206.40, 201.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.50, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.761 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13145, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 154, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (135.25, 54.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.63, 122.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.386 CPU sec, 4.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13115, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.49, 121.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.136 CPU sec, 4.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13104, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 219, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (190.75, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (237.68, 120.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.871 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12993, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 157, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (168.73, 76.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.29, 121.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.011 CPU sec, 5.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13277, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 159, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (197.71, 149.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.14, 120.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.222 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13119, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 124, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (162.88, 171.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (238.86, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.936 CPU sec, 4.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12917, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 145, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (122.29, 52.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.06, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.929 CPU sec, 4.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13169, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 186, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (163.44, 81.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (234.96, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.325 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12968, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 138, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (144.65, 64.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (225.71, 111.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.660 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12918, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 146, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (127.80, 47.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (223.52, 110.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.879 CPU sec, 5.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12651, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 107, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (143.36, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (224.86, 111.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.491 CPU sec, 4.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12335, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 66, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (95.80, 66.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (219.66, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.489 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12540, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 77, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (103.42, 60.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (228.51, 113.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.135 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12984, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 127, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (168.91, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.92, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.839 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12930, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (152.69, 83.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (235.31, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.239 CPU sec, 4.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12939, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 129, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (147.17, 83.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (233.43, 117.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.482 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13089, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 235, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (223.06, 88.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.17, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.819 CPU sec, 4.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12934, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (179.65, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.15, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.296 CPU sec, 5.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13038, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (168.71, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.08, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.171 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12945, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 130, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (145.10, 76.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.59, 119.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.484 CPU sec, 4.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13004, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 151, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (161.67, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.92, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.252 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12977, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 137, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (172.73, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.86, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.315 CPU sec, 5.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13005, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 149, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (177.57, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (237.79, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.240 CPU sec, 4.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12956, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 155, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (171.61, 121.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (225.76, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.754 CPU sec, 5.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12993, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 168, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (150.05, 70.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (225.28, 112.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.234 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12692, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 186, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (164.50, 82.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (222.14, 109.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.180 CPU sec, 4.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12525, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 124, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (197.60, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 189, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (218.34, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.574 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12138, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (155.95, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (220.56, 108.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.210 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12923, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 136, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (145.07, 74.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.46, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.997 CPU sec, 4.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13054, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 122, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (172.29, 123.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (235.99, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.904 CPU sec, 4.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13186, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 134, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (150.62, 110.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (233.63, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.943 CPU sec, 4.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12956, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 153, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (186.32, 156.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.30, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.373 CPU sec, 5.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13136, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 202, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (186.30, 118.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.45, 116.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.884 CPU sec, 4.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13054, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (135.75, 64.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (234.49, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.635 CPU sec, 4.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13059, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 93, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (113.43, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.20, 120.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.981 CPU sec, 3.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12974, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 203, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (244.17, 173.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.05, 121.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.101 CPU sec, 4.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13163, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 134, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (179.13, 147.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.25, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.249 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13078, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 166, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (206.08, 143.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.90, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.398 CPU sec, 4.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13122, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 182, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (185.53, 124.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.65, 118.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.819 CPU sec, 4.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13035, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 158, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.15, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.98, 115.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.290 CPU sec, 4.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12954, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (117.50, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (225.56, 111.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.789 CPU sec, 4.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12677, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 171, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (163.86, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (224.24, 111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.982 CPU sec, 4.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12415, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 184, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (161.46, 70.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (224.01, 110.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.734 CPU sec, 4.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12749, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (162.00, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.01, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.873 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13061, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (220.24, 115.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.32, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.456 CPU sec, 4.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12983, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (151.86, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.56, 120.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.549 CPU sec, 4.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13016, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.11, 81.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.12, 119.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.731 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12937, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 175, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (194.18, 100.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (232.31, 117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.981 CPU sec, 3.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13041, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 178, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (195.41, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.77, 119.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.129 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12954, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 170, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (158.86, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.27, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.737 CPU sec, 4.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12873, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 114, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (146.00, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.76, 119.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.470 CPU sec, 4.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13061, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 212, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (164.73, 96.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.69, 120.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.087 CPU sec, 5.057 real sec [M::process] read 55574 sequences (8391674 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12960, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 162, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (179.50, 108.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.07, 119.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.039 CPU sec, 4.314 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10851, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 174, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (147.56, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (236.53, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 55574 reads in 91.364 CPU sec, 3.596 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21173\tSM:UG10S2P412\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21173_HLKNYBGXX_UG10S2P412_R1.fastq.gz 5347_7332_21173_HLKNYBGXX_UG10S2P412_R2.fastq.gz [main] Real time: 1830.594 sec; CPU: 46165.583 sec Finished alignement of UG10S2P412 at Wed Apr 3 11:02:10 2019 Sorting BAM file started at Wed Apr 3 11:02:10 2019 samtools sort -@ 30 -m 4G UG10S2P412_unsorted.bam -o UG10S2P412.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 11:02:10 2019 rm UG10S2P412_unsorted.bam rm 5347_7332_21173_HLKNYBGXX_UG10S2P412_R1.fastq.gz rm 5347_7332_21173_HLKNYBGXX_UG10S2P412_R2.fastq.gz samtools index UG10S2P412.bam Finished BAM file generation of the sample - UG10S2P412 Processing fastq files for the sample - UG10S2P419 Reading 5347_7332_21174_HLKNYBGXX_UG10S2P419_R1.fastq.gz 5347_7332_21174_HLKNYBGXX_UG10S2P419_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21174\tSM:UG10S2P419\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21174_HLKNYBGXX_UG10S2P419_R1.fastq.gz 5347_7332_21174_HLKNYBGXX_UG10S2P419_R2.fastq.gz | samtools view -Sb1 - > UG10S2P419_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13745, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 170, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (140.67, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.29, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.098 CPU sec, 6.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13691, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 142, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (170.14, 96.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.99, 96.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.333 CPU sec, 5.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13763, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 99, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (119.96, 65.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.11, 96.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.326 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13775, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 120, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (137.10, 81.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.39, 97.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.052 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13695, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (110.21, 52.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.96, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.565 CPU sec, 5.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13925, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 133, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (132.81, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.50, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.490 CPU sec, 4.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13964, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 122, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (152.55, 100.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.02, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.339 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13838, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 131, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (160.88, 107.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (203.39, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.138 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14008, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 124, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (130.90, 62.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.52, 97.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.211 CPU sec, 4.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13859, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 150, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (164.57, 95.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (204.02, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.151 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14090, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (149.12, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.49, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.018 CPU sec, 5.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14045, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 105, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (116.05, 53.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (200.87, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.755 CPU sec, 5.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14087, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 95, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (120.00, 78.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (205.06, 100.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.310 CPU sec, 4.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13941, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 180, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (173.67, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.13, 98.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.772 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13998, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 102, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (142.36, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.26, 97.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.570 CPU sec, 4.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14109, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 204, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (157.63, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (203.75, 98.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.713 CPU sec, 4.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14091, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (147.94, 78.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (203.07, 98.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.703 CPU sec, 4.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13942, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 146, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (166.33, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.46, 97.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.754 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14045, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (146.10, 74.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.36, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.049 CPU sec, 5.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14207, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 152, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (185.96, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.92, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.146 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14080, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 146, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (149.35, 93.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.24, 98.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.481 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13882, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 125, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (143.41, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (200.94, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.924 CPU sec, 5.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14256, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (160.62, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.61, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.445 CPU sec, 4.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13966, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 137, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (152.32, 93.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (199.47, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.686 CPU sec, 4.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13869, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 109, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (124.70, 79.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.88, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.840 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14084, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 95, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (119.58, 73.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.34, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.262 CPU sec, 5.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13975, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 140, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (134.62, 94.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.85, 98.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.481 CPU sec, 4.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13733, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (140.90, 92.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.85, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.283 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14099, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 106, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (143.24, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.99, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.375 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14071, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 101, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (105.83, 55.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (202.64, 98.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.539 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14018, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 123, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (150.14, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.75, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.197 CPU sec, 4.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14082, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (143.69, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.36, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.971 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14151, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 125, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (130.82, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.23, 96.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.342 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14114, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (108.91, 62.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.95, 93.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.628 CPU sec, 4.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13997, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 154, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (150.08, 83.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.27, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.571 CPU sec, 4.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13937, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (175.17, 101.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (198.02, 95.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.922 CPU sec, 6.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13828, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (164.58, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.01, 95.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.633 CPU sec, 4.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14030, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (157.12, 116.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.55, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.337 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14047, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 192, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (156.92, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.38, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.757 CPU sec, 4.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13853, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 128, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (143.28, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.18, 93.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.339 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13830, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 142, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (190.00, 157.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.21, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.052 CPU sec, 5.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13927, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (150.17, 93.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.45, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.943 CPU sec, 4.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13996, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (115.65, 54.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.54, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.409 CPU sec, 5.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13968, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 95, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (100.15, 50.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.19, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.698 CPU sec, 5.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13873, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (126.64, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.16, 93.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.989 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13762, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 121, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (152.36, 110.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.76, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.640 CPU sec, 5.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13704, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (133.67, 85.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.52, 96.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.629 CPU sec, 5.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13583, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 104, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (135.92, 85.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.05, 96.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.032 CPU sec, 4.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13780, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 101, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (123.19, 85.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.60, 96.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.630 CPU sec, 5.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13713, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (155.04, 98.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.04, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.428 CPU sec, 4.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13692, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (153.09, 116.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.32, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.336 CPU sec, 4.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13892, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 159, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (158.74, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (201.31, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.595 CPU sec, 4.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13977, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 128, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (158.47, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (200.71, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.551 CPU sec, 6.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13917, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (143.57, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.08, 95.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.679 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13872, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 95, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (105.67, 51.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.09, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.289 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13935, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 160, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (133.42, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.09, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.274 CPU sec, 4.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14125, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 161, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (165.46, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (202.98, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.968 CPU sec, 4.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13862, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (140.17, 89.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.02, 98.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.924 CPU sec, 4.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14062, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 115, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (138.21, 81.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.41, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.182 CPU sec, 4.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13987, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 91, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (107.12, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (200.89, 97.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.314 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14137, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 140, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (124.00, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.12, 98.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.314 CPU sec, 5.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14053, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 99, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (116.24, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.55, 99.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.816 CPU sec, 5.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14094, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 158, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (156.73, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.91, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.451 CPU sec, 5.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14040, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (129.31, 81.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.41, 96.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.281 CPU sec, 4.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13892, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 174, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (167.17, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.41, 98.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.750 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14159, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (110.88, 49.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.10, 98.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.805 CPU sec, 5.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14147, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 114, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (126.47, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.04, 95.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.429 CPU sec, 6.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13970, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 144, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (155.32, 83.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.26, 99.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.648 CPU sec, 5.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14135, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (137.45, 77.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.56, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.439 CPU sec, 5.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14069, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 116, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (133.82, 80.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.55, 97.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.456 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14072, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 125, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (129.95, 60.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.54, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.683 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14044, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 148, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (138.80, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.40, 94.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.947 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14064, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (148.58, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.80, 96.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.708 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14137, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 176, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (183.91, 99.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.07, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.650 CPU sec, 5.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14040, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 133, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (157.77, 99.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.33, 96.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.390 CPU sec, 4.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14153, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 155, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (150.93, 64.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.97, 94.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.169 CPU sec, 4.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14149, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 107, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (152.45, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.52, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.272 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13966, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (147.88, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.11, 93.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.061 CPU sec, 5.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13978, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 129, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (149.36, 85.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.91, 95.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.422 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13876, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 89, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (126.22, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.38, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.743 CPU sec, 4.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13971, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 116, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (165.08, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.84, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.434 CPU sec, 4.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13871, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 100, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (133.67, 97.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.22, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.235 CPU sec, 5.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14023, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 131, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (131.07, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.45, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.745 CPU sec, 4.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14208, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 168, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (161.45, 85.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.13, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.782 CPU sec, 4.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13884, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 148, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (145.22, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.60, 93.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.493 CPU sec, 4.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13927, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (115.18, 66.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.73, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.830 CPU sec, 5.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13870, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 112, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (130.11, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.24, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.819 CPU sec, 4.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13980, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 160, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (169.42, 110.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.43, 95.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.538 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13949, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 104, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (127.54, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.09, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.688 CPU sec, 4.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14107, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 129, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (146.12, 106.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.50, 92.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.244 CPU sec, 4.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13894, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 140, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (135.38, 76.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.84, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.115 CPU sec, 4.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13657, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 131, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (148.35, 46.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.22, 96.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.195 CPU sec, 4.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13819, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (120.73, 47.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.47, 97.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.700 CPU sec, 5.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13816, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 107, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (142.69, 84.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.54, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.875 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13675, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (144.35, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.56, 96.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.416 CPU sec, 6.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13914, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 96, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (86.00, 37.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.65, 94.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.680 CPU sec, 4.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13794, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 101, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (119.54, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.07, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.423 CPU sec, 4.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13701, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 138, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (125.33, 74.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (199.45, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.830 CPU sec, 4.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13941, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 85, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (100.00, 66.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.73, 95.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.863 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13700, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (113.23, 64.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.55, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.052 CPU sec, 5.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13828, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 111, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (124.83, 61.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.65, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.605 CPU sec, 5.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13881, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (124.81, 77.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.12, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.123 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13733, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (142.17, 67.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.23, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.216 CPU sec, 6.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13904, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 144, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (162.55, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.22, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.502 CPU sec, 4.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13890, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 110, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (106.05, 55.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.71, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.779 CPU sec, 4.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13788, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 133, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (150.43, 91.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.58, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.868 CPU sec, 6.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14017, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 129, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 255) [M::mem_pestat] mean and std.dev: (124.33, 38.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.19, 98.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.779 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13924, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 116, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (129.69, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.50, 96.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.860 CPU sec, 4.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14062, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (168.35, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.28, 98.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.161 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13839, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (153.59, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.85, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.782 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13917, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (131.57, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.83, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.672 CPU sec, 4.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14047, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (151.91, 102.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.00, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.428 CPU sec, 4.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13986, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (126.62, 61.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.48, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.152 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13838, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 146, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (188.31, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.99, 95.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.851 CPU sec, 5.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13793, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 172, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (159.73, 90.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.76, 95.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.759 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14048, 4, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 125, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (137.82, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.02, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.502 CPU sec, 4.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13998, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 124, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (120.93, 57.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.06, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.649 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13914, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (119.15, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.82, 94.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.604 CPU sec, 4.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13998, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (106.53, 47.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.47, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.849 CPU sec, 4.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13997, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 139, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (155.32, 82.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.14, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.172 CPU sec, 4.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13898, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 179, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (178.59, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.91, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.742 CPU sec, 5.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13962, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 113, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (138.00, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.79, 95.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.186 CPU sec, 4.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13962, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (135.32, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (198.12, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.603 CPU sec, 4.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13946, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 126, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.36, 62.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.22, 93.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.489 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14032, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 159, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (167.75, 91.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.08, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.411 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13941, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 114, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (147.87, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.61, 94.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.008 CPU sec, 5.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13878, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 108, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (107.10, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.15, 94.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.733 CPU sec, 5.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14189, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 144, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (163.42, 90.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.67, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.888 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13884, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 142, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (178.95, 100.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.80, 93.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.125 CPU sec, 4.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14063, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 115, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (126.33, 56.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.39, 95.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.728 CPU sec, 4.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13821, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 101, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (109.76, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.94, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.980 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14033, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (185.48, 141.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.76, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.765 CPU sec, 3.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13792, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 136, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (168.06, 121.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.63, 93.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.718 CPU sec, 4.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13734, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (122.19, 57.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.78, 94.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.729 CPU sec, 5.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13850, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (134.00, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.53, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.833 CPU sec, 4.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13663, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 85, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (89.85, 38.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.74, 92.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.338 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13764, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 128, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (147.88, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.00, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.493 CPU sec, 5.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13672, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (121.44, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.24, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.549 CPU sec, 6.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13803, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 128, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (107.43, 48.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.72, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.520 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13949, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 118, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (122.58, 74.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.72, 95.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.229 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13900, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 124, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (119.63, 51.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.41, 89.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.840 CPU sec, 4.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13963, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 147, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (143.45, 58.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.30, 93.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.616 CPU sec, 4.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13806, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 107, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (131.36, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.47, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.077 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13995, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (123.18, 68.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.88, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.832 CPU sec, 4.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13894, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (132.32, 75.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.66, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.188 CPU sec, 4.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14024, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (133.92, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.99, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.755 CPU sec, 4.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14005, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 117, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (133.25, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.31, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.726 CPU sec, 6.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13923, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 124, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (155.41, 82.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.25, 95.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.063 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13841, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 100, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (123.08, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.04, 93.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.432 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14055, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 143, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (137.11, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (201.13, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.922 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14092, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (155.32, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.67, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.025 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13955, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 109, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (126.05, 68.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.12, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.486 CPU sec, 4.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13944, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 154, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (160.68, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.22, 97.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.609 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13966, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 141, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (126.95, 61.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.16, 96.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.812 CPU sec, 5.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14088, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 147, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (150.52, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.42, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.812 CPU sec, 5.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13980, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 118, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (166.00, 104.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.69, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.595 CPU sec, 4.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13944, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 142, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (181.74, 135.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (199.78, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.159 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14038, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (144.96, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.27, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.234 CPU sec, 4.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14011, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 89, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (100.96, 43.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.61, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.455 CPU sec, 4.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14016, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 107, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (147.56, 107.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.00, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.076 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13894, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 114, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (166.44, 166.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.69, 95.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.330 CPU sec, 5.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14218, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 87, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (116.38, 84.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.95, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.594 CPU sec, 4.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13866, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (132.37, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.00, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.643 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14141, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 99, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (148.88, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.52, 94.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.697 CPU sec, 4.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14002, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 108, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (137.36, 84.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.46, 95.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.284 CPU sec, 5.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14059, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 98, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (135.65, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.00, 95.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.448 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13920, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (173.82, 79.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.78, 93.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.651 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14012, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 104, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (135.79, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.26, 92.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.833 CPU sec, 4.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14061, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 88, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (92.84, 44.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.53, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.790 CPU sec, 4.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13843, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 117, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (148.87, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (198.01, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.408 CPU sec, 5.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14052, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 121, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (142.50, 75.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.56, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.874 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14021, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (105.38, 59.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.22, 94.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.541 CPU sec, 4.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13983, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (125.32, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (194.90, 92.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.946 CPU sec, 4.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13885, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 140, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (134.00, 64.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.63, 93.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.667 CPU sec, 5.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13978, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 111, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (118.07, 78.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.43, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.304 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13801, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 150, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (154.90, 76.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.20, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.164 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13897, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 138, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (146.16, 107.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.30, 93.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.998 CPU sec, 4.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13874, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (122.47, 59.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.28, 91.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.090 CPU sec, 4.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13893, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 120, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (115.52, 43.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.78, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.654 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13811, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 119, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (115.61, 68.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.32, 91.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.668 CPU sec, 5.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13690, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (121.64, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.43, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.946 CPU sec, 5.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13512, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 169, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (185.17, 85.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.36, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.426 CPU sec, 5.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13561, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (164.42, 113.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.38, 93.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.949 CPU sec, 5.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13641, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 100, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (139.76, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.89, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.191 CPU sec, 5.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13668, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 164, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (162.68, 81.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.26, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.792 CPU sec, 5.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13930, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (127.33, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.39, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.172 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13851, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 101, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (115.55, 66.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.84, 92.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.003 CPU sec, 5.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13915, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 141, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (136.35, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.77, 92.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.256 CPU sec, 5.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13997, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (156.14, 89.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.22, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.043 CPU sec, 5.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13947, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 123, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (122.09, 76.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.90, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.069 CPU sec, 3.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13836, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (158.76, 81.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.73, 95.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.055 CPU sec, 5.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13867, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 121, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (128.80, 51.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.24, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.291 CPU sec, 4.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13946, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (149.92, 93.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.98, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.309 CPU sec, 4.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13987, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 137, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (121.87, 56.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.82, 97.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.571 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14045, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 107, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (139.35, 79.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.70, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.493 CPU sec, 4.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14090, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 160, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (149.79, 83.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.65, 95.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.529 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14094, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 111, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (126.05, 75.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.85, 95.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.036 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 14087, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.09, 96.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.664 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14048, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (117.72, 48.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.09, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.339 CPU sec, 5.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14046, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 124, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (147.86, 89.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.76, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.041 CPU sec, 4.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13941, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 91, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (111.32, 62.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.98, 95.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.287 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14099, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 91, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (116.07, 77.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.20, 94.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.909 CPU sec, 4.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14043, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 121, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (115.05, 61.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.06, 96.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.236 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14179, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (149.36, 110.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.29, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.917 CPU sec, 4.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13924, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (132.50, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.93, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.222 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14132, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 237, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (215.50, 110.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.42, 94.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.903 CPU sec, 4.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13995, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (126.16, 66.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.02, 93.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.129 CPU sec, 5.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13989, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 130, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (131.78, 63.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (199.95, 96.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.808 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13960, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (127.33, 68.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (197.93, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.346 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14052, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 133, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (138.20, 77.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.94, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.508 CPU sec, 4.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14006, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 94, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (152.48, 125.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.12, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.094 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14042, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 152, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (163.25, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.52, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.440 CPU sec, 4.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13911, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (130.25, 56.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.83, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.571 CPU sec, 4.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14169, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 129, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (124.34, 57.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.12, 93.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.326 CPU sec, 4.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13965, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 86, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (119.95, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.14, 91.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.706 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13998, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (139.06, 80.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.17, 92.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.394 CPU sec, 4.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13809, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 137, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (125.43, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.84, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.981 CPU sec, 4.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13888, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 96, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (125.44, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.87, 92.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.421 CPU sec, 4.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13936, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (128.42, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (196.47, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.561 CPU sec, 4.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13943, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (130.36, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.28, 92.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.903 CPU sec, 5.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13960, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (132.29, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.42, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.446 CPU sec, 5.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13758, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 107, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (116.62, 56.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.22, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.350 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13836, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 114, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (106.76, 56.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (196.35, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.445 CPU sec, 5.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13940, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (134.45, 71.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.30, 91.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.949 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14214, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 155, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (146.41, 60.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.84, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.158 CPU sec, 5.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13802, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 101, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (119.05, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.68, 91.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.723 CPU sec, 4.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13656, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (125.64, 42.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.81, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.237 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13646, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (195.46, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.930 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13743, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 96, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (94.73, 26.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.00, 89.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.403 CPU sec, 4.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13950, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 113, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (120.16, 57.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.64, 94.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.724 CPU sec, 4.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13838, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 95, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (109.61, 64.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.53, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.614 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13880, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 150, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (148.60, 84.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.31, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.890 CPU sec, 4.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13664, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 123, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (151.88, 83.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.45, 95.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.165 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13952, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 93, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (139.79, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.46, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.848 CPU sec, 4.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13788, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 107, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (142.38, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.03, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.856 CPU sec, 4.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13871, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 114, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (119.08, 73.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.65, 92.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.673 CPU sec, 5.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14008, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 115, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (146.41, 81.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.33, 95.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.327 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13973, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (122.95, 63.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.69, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.236 CPU sec, 5.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13838, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 138, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (162.24, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.44, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.905 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13819, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 99, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (137.45, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.19, 94.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.240 CPU sec, 4.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13806, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 118, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (107.83, 43.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.05, 94.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.526 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13875, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 154, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (155.50, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.24, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.329 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13870, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 130, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (146.40, 91.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.04, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.588 CPU sec, 4.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13980, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 157, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (158.07, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.34, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.999 CPU sec, 6.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13954, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 95, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (89.82, 51.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.30, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.044 CPU sec, 4.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14051, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 150, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (174.47, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.80, 96.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.156 CPU sec, 4.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13957, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 90, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (86.52, 37.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.05, 96.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.352 CPU sec, 5.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14150, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 104, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (169.95, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.57, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.938 CPU sec, 6.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14011, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 111, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (132.10, 83.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.45, 94.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.054 CPU sec, 4.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13876, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (130.33, 83.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.40, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.535 CPU sec, 4.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13992, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 175, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (157.60, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.88, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.787 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14030, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 83, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (116.60, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.89, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.230 CPU sec, 4.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14070, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 81, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (87.71, 47.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.32, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.646 CPU sec, 4.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13801, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 98, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (106.38, 53.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.50, 93.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.119 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13853, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 128, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (163.13, 110.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.21, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.360 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13937, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 72, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (82.78, 33.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.34, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.395 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13924, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 121, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (167.26, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.15, 90.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.113 CPU sec, 6.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13932, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 143, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (155.22, 95.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.85, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.811 CPU sec, 4.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13942, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 121, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (144.22, 76.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.98, 91.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.852 CPU sec, 5.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13841, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 113, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (142.52, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.72, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.415 CPU sec, 4.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13998, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 171, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (166.55, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (195.65, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.306 CPU sec, 4.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13829, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 100, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (114.22, 70.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.14, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.564 CPU sec, 5.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14061, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 102, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (143.85, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.05, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.738 CPU sec, 4.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13955, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 102, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (102.38, 42.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.54, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.113 CPU sec, 4.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13838, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (130.32, 64.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.94, 91.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.296 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13894, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 153, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (148.38, 56.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.55, 91.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.698 CPU sec, 4.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13886, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 131, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (133.79, 96.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.03, 88.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.954 CPU sec, 4.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13766, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 131, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (143.60, 82.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.70, 93.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.422 CPU sec, 5.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13781, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (133.37, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.97, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.266 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13762, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (145.39, 90.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.56, 93.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.732 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13903, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 107, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (128.50, 79.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.83, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.731 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14019, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (141.00, 77.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.25, 99.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.090 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13949, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (116.21, 57.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.89, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.105 CPU sec, 4.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13945, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 199, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (218.05, 92.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (203.00, 98.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.590 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13904, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (142.91, 84.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.51, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.506 CPU sec, 4.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13848, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 118, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (123.20, 52.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.79, 96.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.707 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13954, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 156, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (162.57, 74.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.11, 98.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.918 CPU sec, 4.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13917, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 121, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (149.67, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.18, 96.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.991 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13901, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 99, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (105.93, 44.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.09, 97.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.187 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13840, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 112, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (136.67, 69.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.23, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.529 CPU sec, 5.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14111, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 106, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (164.80, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (201.22, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.303 CPU sec, 4.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14080, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 94, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (110.06, 62.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.86, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.666 CPU sec, 6.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13873, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 145, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (181.40, 105.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.99, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.894 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14005, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (180.29, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.12, 97.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.512 CPU sec, 5.785 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14012, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 109, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (110.46, 59.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.17, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.891 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14039, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 101, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (145.13, 115.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.03, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.016 CPU sec, 4.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13986, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 110, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (136.81, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.32, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.705 CPU sec, 5.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13941, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 100, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (102.36, 40.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (201.91, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.552 CPU sec, 4.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13946, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 140, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (135.88, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.77, 96.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.471 CPU sec, 4.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13868, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 128, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (133.00, 66.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.79, 99.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.173 CPU sec, 4.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14048, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (194.00, 97.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.04, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.454 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13836, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 101, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (173.77, 129.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.07, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.771 CPU sec, 4.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14009, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 90, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (111.23, 81.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.70, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.212 CPU sec, 4.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14027, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 148, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (150.53, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.59, 98.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.459 CPU sec, 6.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14030, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 161, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (157.52, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.05, 98.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.815 CPU sec, 4.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14006, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (147.62, 62.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.73, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.864 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13957, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 91, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (108.28, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.30, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.364 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14001, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (131.36, 65.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.71, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.381 CPU sec, 6.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14138, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (152.86, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.99, 97.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.233 CPU sec, 5.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14061, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (133.93, 70.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.72, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.815 CPU sec, 4.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13927, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 151, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (142.00, 66.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.27, 96.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.141 CPU sec, 5.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13858, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (158.20, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.65, 96.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.821 CPU sec, 4.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13924, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 156, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (151.27, 80.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.96, 95.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.827 CPU sec, 5.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14010, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 153, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (169.65, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.06, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.843 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13822, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 175, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (195.93, 141.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.11, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.130 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13894, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 153, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (156.17, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.44, 95.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.513 CPU sec, 5.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13902, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 165, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (180.47, 112.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.47, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.232 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13853, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 92, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (151.76, 124.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.15, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.714 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13891, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 113, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (103.25, 45.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (196.17, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.475 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13816, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 108, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (114.71, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.41, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.236 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13736, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 141, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (148.48, 54.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (195.09, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.755 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13636, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (102.24, 51.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.35, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.853 CPU sec, 5.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13650, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (132.28, 78.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.09, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.333 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13627, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (131.38, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (192.16, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.447 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13266, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (134.81, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (193.28, 90.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.789 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13403, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 145, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (154.87, 76.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.02, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.644 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13846, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 81, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (96.42, 47.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.15, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.698 CPU sec, 4.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13933, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (135.44, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (204.55, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.016 CPU sec, 5.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13845, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 164, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.62, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (206.11, 102.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.254 CPU sec, 4.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13856, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 97, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (135.93, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.40, 98.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.711 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13920, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 142, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (152.88, 100.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (205.40, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.760 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13842, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (136.20, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.49, 97.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.416 CPU sec, 8.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14052, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 132, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (154.90, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (205.47, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.735 CPU sec, 4.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14081, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (123.24, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (205.45, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.876 CPU sec, 4.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13987, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 234, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (210.13, 94.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.80, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.852 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13856, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 97, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (119.40, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (205.34, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.301 CPU sec, 5.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13968, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (175.12, 112.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.21, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.492 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13980, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (130.44, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (206.09, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.721 CPU sec, 4.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13928, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 141, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (142.24, 86.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.03, 100.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.067 CPU sec, 5.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13923, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 126, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (144.47, 78.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (207.38, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.487 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14062, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 114, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (137.18, 104.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.26, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.354 CPU sec, 4.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13853, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 115, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (155.24, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (203.98, 99.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.374 CPU sec, 4.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13851, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 127, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (151.45, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (204.74, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.435 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14054, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (137.33, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (205.01, 101.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.761 CPU sec, 5.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13952, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 118, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (129.38, 69.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.37, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.887 CPU sec, 5.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13976, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (112.40, 56.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.01, 98.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.697 CPU sec, 5.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14013, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 132, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (132.42, 56.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (203.56, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.038 CPU sec, 5.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13909, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 118, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (154.63, 108.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.94, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.928 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14076, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 131, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (146.46, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.33, 100.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.312 CPU sec, 4.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13991, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 156, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (158.00, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.11, 99.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.377 CPU sec, 4.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14030, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 120, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (139.65, 75.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (201.88, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.202 CPU sec, 4.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13936, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 148, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (159.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.03, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.029 CPU sec, 4.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14077, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 138, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (148.83, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (199.99, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.603 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14072, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (127.95, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.78, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.805 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14095, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 91, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (104.17, 59.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.46, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.389 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13928, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 150, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (153.11, 83.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.47, 98.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.673 CPU sec, 4.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14063, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 128, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (153.20, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.41, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.080 CPU sec, 4.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13952, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (125.94, 61.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.97, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.507 CPU sec, 7.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13981, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 207, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (210.95, 150.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.17, 94.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.377 CPU sec, 4.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13942, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 140, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (147.78, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.47, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.593 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13786, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 106, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (116.70, 74.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.25, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.055 CPU sec, 5.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13811, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 149, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (137.81, 65.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.14, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.903 CPU sec, 5.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13823, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 119, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (129.80, 80.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.39, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.847 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13834, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 132, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (136.59, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.70, 90.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.674 CPU sec, 4.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13632, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 84, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (108.43, 64.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.60, 90.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.482 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13477, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 91, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (99.76, 61.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.05, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.056 CPU sec, 5.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13678, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (130.45, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.63, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.071 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13486, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 103, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (135.12, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.14, 89.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.721 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13405, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 128, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (145.00, 86.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.38, 87.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.424 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13278, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 159, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (156.92, 83.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.11, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.231 CPU sec, 5.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13135, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 93, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (96.53, 48.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.73, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.325 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13776, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 114, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (130.59, 80.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (204.17, 99.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.720 CPU sec, 4.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13926, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 107, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (112.43, 57.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (203.83, 99.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.716 CPU sec, 4.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13886, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (149.40, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.30, 99.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.639 CPU sec, 4.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13994, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 121, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (151.17, 84.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (205.03, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.925 CPU sec, 5.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13899, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (182.50, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.54, 99.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.410 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13942, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 149, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (131.71, 59.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (205.26, 101.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.869 CPU sec, 4.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13879, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (199.96, 144.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (203.77, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.526 CPU sec, 4.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13985, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (139.68, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (204.09, 98.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.098 CPU sec, 4.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13940, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 176, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (164.74, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.37, 99.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.824 CPU sec, 4.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13880, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 105, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (126.50, 113.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.78, 100.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.337 CPU sec, 4.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13933, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 183, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (178.52, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.50, 99.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.346 CPU sec, 6.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14024, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (118.36, 63.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (202.08, 98.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.394 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13955, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (101.50, 58.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (205.54, 100.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.076 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14033, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 90, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (114.71, 65.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (203.54, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.675 CPU sec, 4.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14008, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 149, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (158.40, 78.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.98, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.713 CPU sec, 4.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13907, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 128, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (140.70, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (202.06, 99.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.785 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13757, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (147.00, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.72, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.038 CPU sec, 4.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13998, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 69, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (71.05, 33.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.45, 98.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.565 CPU sec, 4.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14002, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 116, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (110.50, 48.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.46, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.420 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14089, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 90, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (125.96, 86.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.55, 99.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.948 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13924, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 136, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (156.83, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.36, 99.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.476 CPU sec, 5.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14049, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 139, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (145.65, 72.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.43, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.521 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13793, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 146, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (145.84, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (202.91, 99.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.481 CPU sec, 3.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14099, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (139.46, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.93, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.753 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14046, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 126, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (121.13, 52.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.71, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.594 CPU sec, 5.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13901, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 97, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (98.18, 52.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.07, 96.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.207 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13888, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 111, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (113.47, 53.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.70, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.490 CPU sec, 5.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14034, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 104, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (117.41, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.50, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.690 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13989, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (144.50, 71.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.66, 96.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.400 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14013, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 162, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (162.33, 99.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.71, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.678 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14039, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 142, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (141.55, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.11, 94.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.014 CPU sec, 5.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13971, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 86, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (91.63, 52.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.19, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.311 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13906, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 230, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (220.20, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (195.83, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.609 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14059, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 120, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (126.52, 61.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.01, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.469 CPU sec, 5.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13800, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (144.15, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.42, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.647 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13937, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 111, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (135.26, 86.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.47, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.124 CPU sec, 5.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13701, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 103, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (124.19, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.41, 92.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.808 CPU sec, 5.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13774, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 143, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (142.21, 69.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.82, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.716 CPU sec, 4.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13645, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 102, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (156.29, 94.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.47, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.750 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13636, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (175.43, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.56, 89.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.326 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13599, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 124, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (136.36, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.60, 91.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.261 CPU sec, 4.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13399, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 113, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (116.11, 61.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.83, 88.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.590 CPU sec, 6.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13266, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 118, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (143.91, 95.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (192.79, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.501 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13269, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (127.08, 59.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.57, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.366 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13476, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (116.67, 63.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.41, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.407 CPU sec, 5.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13933, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 120, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (129.81, 65.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.31, 95.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.140 CPU sec, 4.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13847, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 142, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (155.53, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.37, 98.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.126 CPU sec, 6.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13833, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 97, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (125.70, 90.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.68, 98.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.452 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13935, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (175.69, 165.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.48, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.835 CPU sec, 4.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13981, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 107, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (123.18, 58.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.45, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.500 CPU sec, 4.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13951, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (124.03, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.55, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.342 CPU sec, 4.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13768, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 100, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (119.78, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.99, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.677 CPU sec, 6.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13879, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (160.06, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.02, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.518 CPU sec, 4.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13883, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 133, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (169.04, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.64, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.315 CPU sec, 4.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13826, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 199, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (179.70, 108.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.87, 95.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.231 CPU sec, 5.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13829, 10, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 102, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (104.61, 51.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.86, 96.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 7226, 7969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23165) [M::mem_pestat] mean and std.dev: (5229.30, 3415.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30763) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 123.127 CPU sec, 6.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13942, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 139, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (141.39, 61.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.79, 96.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.101 CPU sec, 4.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13767, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 97, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (89.58, 33.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.12, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.114 CPU sec, 4.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14049, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 135, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (139.86, 79.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.17, 94.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.701 CPU sec, 4.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13907, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 115, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 239) [M::mem_pestat] mean and std.dev: (107.10, 46.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.31, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.661 CPU sec, 4.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14051, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 95, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (122.56, 83.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.69, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.938 CPU sec, 4.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14041, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 83, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151) [M::mem_pestat] mean and std.dev: (78.31, 24.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.38, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.018 CPU sec, 4.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13895, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (167.50, 113.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.14, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.636 CPU sec, 5.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14061, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 87, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (126.50, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.98, 96.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.030 CPU sec, 4.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13962, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (152.71, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.13, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.034 CPU sec, 4.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13995, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 184, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (160.22, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.65, 95.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.631 CPU sec, 4.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13920, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 94, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (120.06, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.04, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.550 CPU sec, 4.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13902, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 94, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (104.06, 48.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.30, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.885 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13997, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 107, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (117.94, 64.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.12, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.319 CPU sec, 4.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13809, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (137.12, 80.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.44, 96.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.675 CPU sec, 4.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13944, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 114, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (146.23, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.85, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.418 CPU sec, 4.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13939, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 118, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (143.47, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.33, 92.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.968 CPU sec, 4.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13827, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 94, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (111.04, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.10, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.718 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13873, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 98, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (159.71, 105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.83, 95.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.036 CPU sec, 4.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14038, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 181, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (170.15, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.79, 92.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.629 CPU sec, 4.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13991, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 127, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (147.29, 79.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.51, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.850 CPU sec, 5.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13925, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (129.26, 43.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.60, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.526 CPU sec, 4.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13944, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 153, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (163.09, 110.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.96, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.260 CPU sec, 4.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13781, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 114, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (139.48, 86.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.09, 89.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.253 CPU sec, 4.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13992, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 158, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (154.24, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.00, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.353 CPU sec, 4.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13849, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 182, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (176.42, 79.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.15, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.427 CPU sec, 4.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13782, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 144, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (137.70, 79.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.81, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.808 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13770, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 77, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (81.94, 41.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.01, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.377 CPU sec, 4.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13864, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 125, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (123.29, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.36, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.543 CPU sec, 5.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13705, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (130.03, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.86, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.793 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13722, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 215, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (197.09, 99.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.50, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.118 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13540, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 134, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (138.15, 76.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.83, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.737 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13565, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 149, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (178.35, 136.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.68, 85.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.164 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13164, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (136.33, 59.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.47, 86.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.708 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13346, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 116, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (114.14, 49.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (190.07, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.565 CPU sec, 5.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13973, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 118, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (153.76, 87.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (201.97, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.983 CPU sec, 5.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14063, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 120, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (114.59, 58.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.22, 98.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.226 CPU sec, 4.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13739, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 136, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (130.81, 91.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.41, 99.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.399 CPU sec, 5.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13937, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 128, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (147.47, 83.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.28, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.297 CPU sec, 5.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13938, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 131, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (134.94, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.99, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.480 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13951, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 108, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (129.53, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.84, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.453 CPU sec, 5.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13982, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 124, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (149.33, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.08, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.913 CPU sec, 6.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13919, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 196, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (178.35, 89.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.23, 98.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.810 CPU sec, 6.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13968, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (137.27, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.39, 96.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.914 CPU sec, 4.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14063, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 94, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (95.53, 44.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.82, 98.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.289 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14057, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 92, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (118.81, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.68, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.157 CPU sec, 4.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14038, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 165, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (164.50, 87.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.82, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.038 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14061, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 100, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (166.94, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.25, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.642 CPU sec, 4.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14040, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (145.83, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.05, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.700 CPU sec, 6.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14041, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (146.56, 69.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.98, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.279 CPU sec, 4.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13952, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 117, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (131.61, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.30, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.494 CPU sec, 5.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14154, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 133, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (132.95, 83.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.85, 96.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.078 CPU sec, 5.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14047, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (173.85, 101.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.66, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.235 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13955, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 143, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (150.12, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.12, 98.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.652 CPU sec, 4.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13998, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 120, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (162.05, 92.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.22, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.318 CPU sec, 4.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13722, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 131, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (201.47, 155.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.27, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.725 CPU sec, 4.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14023, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 112, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (142.82, 72.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.95, 97.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.393 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14146, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (156.41, 106.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.92, 98.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.804 CPU sec, 4.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13910, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 115, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (140.65, 77.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.03, 93.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.734 CPU sec, 4.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13964, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 196, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (186.33, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.65, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.812 CPU sec, 4.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14083, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 156, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (172.40, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.19, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.353 CPU sec, 4.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14019, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 86, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (98.55, 58.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.89, 95.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.971 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13880, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 119, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (124.11, 74.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (197.70, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.322 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13888, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.78, 75.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.22, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.260 CPU sec, 4.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13925, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 94, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (104.00, 64.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.51, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.541 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13947, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (141.80, 83.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.48, 92.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.139 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13965, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 88, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (127.47, 99.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.96, 90.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.914 CPU sec, 4.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14089, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 121, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (167.90, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.17, 90.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.101 CPU sec, 4.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13903, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 129, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (157.15, 88.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.56, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.660 CPU sec, 4.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13914, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (154.83, 115.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.95, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.972 CPU sec, 3.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13787, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 123, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (137.16, 89.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.28, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.144 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13943, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 160, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (157.59, 101.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.49, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.730 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13712, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 99, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (122.00, 76.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.26, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.274 CPU sec, 4.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13815, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 174, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (176.55, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.23, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.295 CPU sec, 5.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13639, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 127, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (108.52, 47.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.79, 87.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.427 CPU sec, 4.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13585, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 116, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (124.00, 57.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.89, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.340 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13460, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (134.87, 68.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.93, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.868 CPU sec, 4.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13179, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 152, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (166.90, 80.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.63, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.835 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13047, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 114, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (144.21, 83.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.29, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.943 CPU sec, 6.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13401, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (110.96, 65.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.77, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.922 CPU sec, 4.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13853, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (127.69, 65.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (206.01, 101.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.958 CPU sec, 4.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13910, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (164.06, 65.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.29, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.921 CPU sec, 4.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13899, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 112, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (132.68, 61.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.79, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.671 CPU sec, 4.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13892, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (136.74, 70.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.26, 98.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.865 CPU sec, 6.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13860, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (129.93, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.79, 99.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.748 CPU sec, 4.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13825, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 88, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (130.92, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.38, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.643 CPU sec, 4.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13914, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 80, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (130.82, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.07, 98.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.852 CPU sec, 5.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13841, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 94, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (107.26, 58.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.39, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.832 CPU sec, 5.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13895, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 94, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (127.29, 64.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.83, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.789 CPU sec, 4.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13873, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 103, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (125.48, 75.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (200.93, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.282 CPU sec, 4.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13936, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 76, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (79.18, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.50, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.263 CPU sec, 5.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13900, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 116, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (110.43, 48.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.62, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.920 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13965, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 93, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (136.85, 100.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.15, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.802 CPU sec, 4.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13856, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (123.50, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.62, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.617 CPU sec, 4.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13860, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (174.00, 69.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.24, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.800 CPU sec, 4.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13864, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 134, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (156.79, 110.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.27, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.848 CPU sec, 4.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13768, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 102, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (112.74, 64.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.38, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.432 CPU sec, 4.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13923, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (154.29, 102.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (204.80, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.596 CPU sec, 5.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14051, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 90, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (95.00, 39.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.59, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.024 CPU sec, 5.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14056, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 82, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (74.38, 34.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.89, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.413 CPU sec, 4.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14094, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 171, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (166.08, 70.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.32, 95.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.428 CPU sec, 4.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13868, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (117.83, 72.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.28, 93.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.596 CPU sec, 4.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14039, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 118, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (148.41, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.61, 94.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.259 CPU sec, 4.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13896, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 127, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (162.39, 104.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.26, 94.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.815 CPU sec, 4.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13980, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (125.10, 56.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.72, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.406 CPU sec, 4.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13961, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (144.24, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.22, 94.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.737 CPU sec, 5.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13857, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 106, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (108.06, 51.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.33, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.982 CPU sec, 5.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14026, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (147.95, 107.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.13, 93.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.400 CPU sec, 6.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13902, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 162, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (166.50, 78.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.83, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.378 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13852, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 97, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (108.50, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.61, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.520 CPU sec, 4.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14008, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (125.09, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (194.88, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.156 CPU sec, 4.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13805, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 95, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (105.74, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.77, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.466 CPU sec, 4.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13866, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (113.75, 60.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (193.42, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.050 CPU sec, 5.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13982, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 137, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (129.86, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (194.14, 91.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.045 CPU sec, 4.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13802, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 124, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (135.48, 74.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.24, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.794 CPU sec, 4.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13943, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 152, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (162.77, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.06, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.186 CPU sec, 7.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13804, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 133, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (143.58, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (191.95, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.570 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13755, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (154.79, 112.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.43, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.691 CPU sec, 4.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13697, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 122, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 188) [M::mem_pestat] mean and std.dev: (112.71, 29.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.51, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.559 CPU sec, 4.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13491, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 134, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (141.60, 105.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.67, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.540 CPU sec, 4.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13791, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 85, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (127.36, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.09, 85.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.121 CPU sec, 4.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13463, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (145.27, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (190.53, 87.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.427 CPU sec, 4.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13443, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (136.12, 82.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.50, 84.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.406 CPU sec, 5.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13392, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (130.16, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.70, 84.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.280 CPU sec, 5.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13524, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (141.95, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.75, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.218 CPU sec, 5.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14112, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 164, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (158.29, 65.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.50, 93.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.954 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13865, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 139, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (128.28, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.48, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.798 CPU sec, 4.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13934, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 140, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (175.87, 109.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.89, 94.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.919 CPU sec, 4.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14054, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 138, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (126.45, 62.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.43, 92.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.558 CPU sec, 3.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14055, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.84, 71.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.26, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.008 CPU sec, 4.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14101, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 91, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (129.43, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.26, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.771 CPU sec, 4.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13873, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 101, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (116.33, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.81, 92.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.862 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13999, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (135.31, 77.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.68, 93.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.726 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13899, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (113.61, 48.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.32, 92.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.045 CPU sec, 4.626 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13941, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (139.07, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.51, 92.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.766 CPU sec, 4.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13764, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (122.36, 65.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.28, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.357 CPU sec, 4.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13930, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 97, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (161.77, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.08, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.978 CPU sec, 4.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13963, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 149, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (155.07, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (197.41, 93.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.930 CPU sec, 4.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13991, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (150.25, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.87, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.527 CPU sec, 3.631 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14040, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (94.41, 44.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.10, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.971 CPU sec, 4.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13912, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 119, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (127.86, 54.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (200.83, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.637 CPU sec, 3.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14144, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 115, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (125.00, 59.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.10, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.079 CPU sec, 4.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14196, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (116.35, 60.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.96, 98.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.755 CPU sec, 3.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14048, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 149, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (145.57, 61.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.32, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.438 CPU sec, 3.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14060, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 168, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (165.50, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.34, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.214 CPU sec, 4.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14059, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (184.70, 110.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.74, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.960 CPU sec, 4.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14002, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 175, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 373) [M::mem_pestat] mean and std.dev: (167.45, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (202.97, 99.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.828 CPU sec, 4.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14099, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (174.65, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.26, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.530 CPU sec, 3.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14044, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 86, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (139.82, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.19, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.623 CPU sec, 3.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13894, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (125.62, 77.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.15, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.987 CPU sec, 4.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14172, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 103, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (136.16, 91.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.86, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.361 CPU sec, 5.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14142, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 175, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (177.00, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.30, 96.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.654 CPU sec, 5.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13947, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 139, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (172.67, 94.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.16, 93.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.738 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13677, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 93, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (120.38, 71.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.14, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.872 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13579, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (169.80, 173.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.12, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.943 CPU sec, 4.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13771, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 138, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (150.44, 85.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.62, 93.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.948 CPU sec, 4.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13810, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 153, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (167.85, 108.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (197.48, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.881 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13728, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 194, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (162.87, 91.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.47, 96.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.610 CPU sec, 4.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13906, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 164, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (168.05, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.36, 93.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.207 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13784, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (126.92, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.49, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.939 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14081, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (125.39, 60.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.01, 94.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.048 CPU sec, 4.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13963, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (114.62, 63.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.68, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.575 CPU sec, 4.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13971, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 158, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (130.96, 62.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.73, 95.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.012 CPU sec, 4.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14128, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 123, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (152.58, 90.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.52, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.331 CPU sec, 4.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13979, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 121, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (139.46, 80.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.74, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.371 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14146, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 143, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (160.25, 93.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.17, 96.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.048 CPU sec, 4.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13893, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (149.84, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.37, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.274 CPU sec, 4.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13892, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 105, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (120.38, 65.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (201.52, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.188 CPU sec, 4.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14029, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 149, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (148.63, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.63, 96.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.723 CPU sec, 4.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14008, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 134, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (159.00, 109.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.15, 95.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.443 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13906, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 114, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (145.12, 87.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (197.82, 94.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.318 CPU sec, 4.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13992, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (124.74, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.52, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 88.469 CPU sec, 3.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13933, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 110, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (115.39, 68.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.70, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.192 CPU sec, 4.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13771, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 122, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (151.38, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.19, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 93.107 CPU sec, 3.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13963, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 118, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (111.75, 70.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.16, 91.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 90.244 CPU sec, 3.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13885, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (124.40, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.57, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 91.550 CPU sec, 3.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13919, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (126.30, 91.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.76, 90.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 91.102 CPU sec, 3.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13871, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 160, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (203.58, 158.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.42, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 87.230 CPU sec, 2.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13925, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 101, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (138.17, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.61, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 89.492 CPU sec, 3.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13839, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (124.88, 69.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (195.25, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 90.538 CPU sec, 3.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13897, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (137.09, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.50, 91.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 89.011 CPU sec, 3.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13987, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 122, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (132.50, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.74, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 91.890 CPU sec, 3.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14192, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (157.10, 94.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.52, 98.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 89.651 CPU sec, 3.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13990, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 106, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (114.44, 53.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.70, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 86.394 CPU sec, 3.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13996, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 167, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (170.18, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.81, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 82.143 CPU sec, 3.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14115, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 102, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (120.77, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.94, 94.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 82.539 CPU sec, 3.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14079, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 110, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (118.44, 45.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (199.74, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 91.890 CPU sec, 4.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13922, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 136, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (161.56, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.85, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 91.983 CPU sec, 3.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14128, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 141, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (163.09, 98.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.03, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.113 CPU sec, 3.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14012, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (158.77, 100.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.34, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 93.231 CPU sec, 3.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14086, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 96, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (126.89, 76.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (201.75, 98.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 97.116 CPU sec, 3.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13932, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (135.25, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (201.37, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.116 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13875, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 158, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (145.91, 103.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.23, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.041 CPU sec, 6.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14067, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 124, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (152.52, 98.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.33, 99.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.277 CPU sec, 4.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14016, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (99.35, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.87, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.243 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14064, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 106, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (109.33, 57.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.37, 94.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.078 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13978, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 202, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (168.00, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.39, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.723 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13672, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 118, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (125.94, 56.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.28, 97.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.092 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13653, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (134.29, 68.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.46, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.091 CPU sec, 4.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13812, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 95, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (89.63, 48.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.63, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 97.615 CPU sec, 3.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13774, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (139.73, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.48, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.772 CPU sec, 4.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13924, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 152, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (163.78, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.94, 96.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.378 CPU sec, 3.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13755, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.46, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 100.335 CPU sec, 3.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13902, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (124.94, 53.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.12, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.336 CPU sec, 3.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13899, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (135.20, 79.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.09, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.342 CPU sec, 4.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14043, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 129, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (146.68, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.40, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 97.418 CPU sec, 3.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13999, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 112, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (123.56, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.63, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.977 CPU sec, 3.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13934, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (149.62, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.85, 95.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.096 CPU sec, 3.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14040, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 90, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (95.47, 53.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.83, 95.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 92.158 CPU sec, 3.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13844, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 142, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (149.44, 81.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.93, 94.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 96.726 CPU sec, 3.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14101, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 200, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (237.00, 200.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.87, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.161 CPU sec, 3.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14040, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (129.00, 83.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.21, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 94.450 CPU sec, 3.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14025, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 131, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (122.15, 75.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.29, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.104 CPU sec, 3.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13913, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 140, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (124.09, 58.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.83, 92.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.685 CPU sec, 3.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14149, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (124.00, 83.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.56, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.448 CPU sec, 3.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14040, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 140, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (140.68, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.64, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.141 CPU sec, 5.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13904, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 166, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (159.47, 103.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.96, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.282 CPU sec, 4.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14065, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 125, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (130.09, 67.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (195.09, 91.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.434 CPU sec, 4.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13996, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 158, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (158.55, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.33, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.126 CPU sec, 4.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13859, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (147.43, 70.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.79, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 96.861 CPU sec, 4.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13914, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (164.73, 115.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.69, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.618 CPU sec, 3.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13957, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (108.45, 48.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.07, 91.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.240 CPU sec, 4.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13994, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 99, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (116.75, 68.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.75, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.659 CPU sec, 3.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13928, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (104.78, 57.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (195.60, 92.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 93.453 CPU sec, 3.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14015, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 121, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (139.88, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.18, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.112 CPU sec, 3.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14064, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 185, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (166.46, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.65, 99.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 96.149 CPU sec, 3.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14007, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (142.05, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.13, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.208 CPU sec, 3.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14076, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (133.29, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.93, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 96.330 CPU sec, 3.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13936, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 154, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (134.80, 59.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.05, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.338 CPU sec, 3.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14050, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 97, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (93.62, 32.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.15, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.131 CPU sec, 3.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14195, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 101, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (113.75, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (202.62, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.053 CPU sec, 4.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14044, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 102, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (132.37, 79.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.76, 96.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.636 CPU sec, 4.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13963, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (144.73, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.70, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.933 CPU sec, 4.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13950, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 104, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (135.20, 83.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.72, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.140 CPU sec, 3.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14199, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 137, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (174.71, 108.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.81, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.785 CPU sec, 3.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13931, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 111, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (113.03, 47.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.43, 97.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 92.113 CPU sec, 3.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14111, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 97, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (91.35, 35.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.05, 96.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.412 CPU sec, 3.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13973, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (155.89, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.56, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 95.504 CPU sec, 3.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13886, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 83, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (103.59, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.66, 91.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.447 CPU sec, 4.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13929, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 164, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (183.58, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.66, 91.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.491 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13782, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 133, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (179.95, 129.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.16, 94.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.317 CPU sec, 4.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13497, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (153.52, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.05, 94.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.035 CPU sec, 4.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13639, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 146, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (154.95, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.20, 93.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.172 CPU sec, 4.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13668, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 129, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (120.00, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.76, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.026 CPU sec, 3.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13833, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 144, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (173.18, 105.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.59, 94.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.597 CPU sec, 4.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13973, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 127, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (128.92, 61.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.34, 94.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.358 CPU sec, 4.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13723, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 128, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (150.65, 101.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.20, 96.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 99.453 CPU sec, 3.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13922, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 147, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (163.32, 91.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.83, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 97.919 CPU sec, 3.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13926, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (146.15, 93.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.74, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 94.793 CPU sec, 3.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14028, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 128, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (145.22, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.42, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.524 CPU sec, 3.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14237, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 152, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (150.72, 66.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.04, 97.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 98.436 CPU sec, 4.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14001, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (138.21, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (201.31, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.628 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13988, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 88, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (111.92, 59.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.12, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.745 CPU sec, 4.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14100, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (151.09, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.44, 96.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.619 CPU sec, 4.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14079, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 121, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (146.35, 83.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (202.86, 98.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.336 CPU sec, 4.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13999, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (110.14, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.61, 93.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.219 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 13693, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 115, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (149.90, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.07, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.266 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13760, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (117.88, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.05, 92.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.490 CPU sec, 4.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13825, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 114, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (122.21, 51.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.25, 90.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.169 CPU sec, 4.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13892, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 134, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (153.22, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.12, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.120 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14126, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (135.08, 77.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.93, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.237 CPU sec, 6.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13916, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 119, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (128.17, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.37, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.372 CPU sec, 6.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13836, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (154.00, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.96, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.320 CPU sec, 4.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13860, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 129, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (144.85, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (195.31, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.729 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13813, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 124, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (144.26, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.72, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.414 CPU sec, 6.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14027, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 134, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (140.60, 55.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.90, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.482 CPU sec, 4.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13786, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (166.25, 91.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.08, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.504 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13782, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.28, 85.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.35, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.081 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14025, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (144.46, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.63, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.363 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13848, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 113, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (108.08, 48.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.59, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.467 CPU sec, 4.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14047, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 99, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (123.80, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.28, 94.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.803 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13979, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 123, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (138.73, 68.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.17, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.411 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14186, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 125, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (152.94, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.60, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.502 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13993, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 125, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (135.41, 63.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.03, 96.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.010 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14146, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 139, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.48, 92.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.13, 93.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.446 CPU sec, 4.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14151, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 149, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (150.90, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (199.76, 97.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.079 CPU sec, 4.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13895, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 137, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (133.78, 59.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.92, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.627 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14112, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (114.05, 68.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.06, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.195 CPU sec, 4.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13996, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 102, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (123.03, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.61, 95.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.135 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13862, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 103, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (107.06, 47.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.24, 94.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.664 CPU sec, 6.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13899, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 82, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (108.46, 66.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.39, 94.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.451 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14010, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 101, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (112.14, 62.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.83, 93.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.780 CPU sec, 4.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14014, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 93, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (113.41, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.63, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.191 CPU sec, 4.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13803, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 110, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (132.08, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.93, 90.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.049 CPU sec, 5.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13793, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (140.95, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.76, 91.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.632 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13717, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (125.12, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.16, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.267 CPU sec, 4.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13753, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 94, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (100.80, 50.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.90, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.623 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13801, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 112, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (150.17, 104.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.72, 90.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.961 CPU sec, 3.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13809, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (134.06, 64.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.48, 91.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.460 CPU sec, 4.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13884, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (132.46, 57.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.72, 90.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.924 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13995, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (159.26, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.29, 93.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.882 CPU sec, 5.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13912, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 118, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (123.78, 70.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.02, 93.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.187 CPU sec, 5.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14027, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 159, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (157.14, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.50, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.152 CPU sec, 4.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13962, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (133.95, 61.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.78, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.306 CPU sec, 5.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13868, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 130, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (128.12, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.43, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.385 CPU sec, 4.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13995, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 126, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (158.25, 95.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.75, 94.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.331 CPU sec, 4.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14038, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 102, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (122.14, 59.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.47, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.441 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14007, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (134.52, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (197.49, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.802 CPU sec, 4.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14155, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 132, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (125.69, 55.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.07, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.647 CPU sec, 4.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14011, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 173, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (174.00, 104.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.71, 89.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.371 CPU sec, 4.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13989, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 136, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (145.39, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.71, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.879 CPU sec, 4.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13981, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 106, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (125.00, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.38, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.351 CPU sec, 4.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14005, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 173, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (158.07, 78.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (195.83, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.449 CPU sec, 4.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14133, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 180, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (162.68, 75.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.89, 92.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.446 CPU sec, 4.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13953, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 143, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (150.45, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.43, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.118 CPU sec, 5.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13976, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 115, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (114.89, 65.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.61, 91.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.244 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13913, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 91, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (107.11, 58.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.89, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.886 CPU sec, 4.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13852, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (177.70, 154.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.16, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.486 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13914, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (155.43, 98.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.68, 91.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.946 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13847, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 110, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (117.06, 58.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.96, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.324 CPU sec, 4.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13901, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (130.57, 84.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (193.95, 90.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.680 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14009, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 118, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (114.00, 60.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.37, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.879 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13949, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 172, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (190.78, 126.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.31, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.238 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14083, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 145, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (171.06, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.86, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.052 CPU sec, 4.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14104, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 99, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (131.25, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.03, 95.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.706 CPU sec, 6.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13995, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 184, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (219.76, 163.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.68, 95.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.583 CPU sec, 4.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14026, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 142, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (171.55, 122.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.15, 93.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.681 CPU sec, 4.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14141, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (137.92, 81.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.03, 94.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.725 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13911, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (121.62, 41.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.11, 96.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.194 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13992, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 113, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (154.38, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.00, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.990 CPU sec, 4.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13963, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (131.14, 70.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.24, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.789 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14000, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (140.00, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.72, 94.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.963 CPU sec, 5.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14019, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 158, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (173.15, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.29, 95.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.961 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14019, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 152, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (158.20, 79.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.69, 93.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.078 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14124, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 138, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (151.37, 68.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.82, 92.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.473 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14053, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 117, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (119.84, 54.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.86, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.852 CPU sec, 3.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14050, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 125, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (149.67, 99.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.29, 92.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.635 CPU sec, 4.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13758, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (143.67, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.25, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.633 CPU sec, 5.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13762, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 151, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (150.60, 62.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.20, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.945 CPU sec, 4.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13767, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 170, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (157.79, 74.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (192.88, 89.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.209 CPU sec, 3.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13727, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 142, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (161.87, 101.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.76, 91.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.606 CPU sec, 4.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13721, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 148, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (136.64, 61.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.03, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.770 CPU sec, 4.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13742, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 164, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (174.87, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.31, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.547 CPU sec, 4.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13850, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (161.12, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.17, 92.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.819 CPU sec, 4.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13869, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 123, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (149.08, 79.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (196.16, 93.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.245 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14075, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 135, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (140.58, 94.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.21, 93.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.488 CPU sec, 4.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14166, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 102, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (111.78, 64.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.78, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.107 CPU sec, 4.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13955, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 184, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (176.84, 89.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (198.25, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.447 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14161, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 124, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (162.88, 133.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.00, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.117 CPU sec, 4.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14068, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (143.43, 96.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.87, 93.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.792 CPU sec, 4.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14068, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (154.38, 86.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (198.36, 95.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.064 CPU sec, 3.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14095, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 143, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (159.19, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.39, 95.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.160 CPU sec, 4.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13984, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 189, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (195.65, 119.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.49, 92.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.065 CPU sec, 4.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13886, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 102, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (129.18, 104.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.63, 91.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.938 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14057, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 142, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (174.95, 102.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.90, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.589 CPU sec, 5.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13906, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (181.25, 110.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.62, 91.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.004 CPU sec, 4.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13884, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (140.73, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (195.56, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.730 CPU sec, 4.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13723, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 99, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (131.58, 87.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.38, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.932 CPU sec, 5.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13939, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 102, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (111.00, 66.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.21, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.491 CPU sec, 4.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14031, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 162, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (160.11, 95.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (191.22, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.126 CPU sec, 4.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13948, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 106, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (129.93, 61.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.24, 89.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.338 CPU sec, 4.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13958, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (138.21, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.72, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.278 CPU sec, 4.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13989, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 173, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (176.25, 112.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.52, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.859 CPU sec, 4.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13949, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 135, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (145.31, 70.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.83, 90.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.693 CPU sec, 4.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13821, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 111, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (121.95, 53.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.40, 91.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.002 CPU sec, 4.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13735, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 119, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (140.55, 86.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.63, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.884 CPU sec, 4.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13909, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 114, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (127.45, 60.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.71, 94.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.700 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14073, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 112, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (111.00, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.61, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.317 CPU sec, 4.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13900, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (133.40, 67.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.15, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.927 CPU sec, 4.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14089, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 119, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.25, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.44, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.604 CPU sec, 4.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14159, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (174.94, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.80, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.978 CPU sec, 4.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14078, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 129, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (147.83, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.50, 94.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.923 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13944, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (111.00, 63.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.37, 93.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.838 CPU sec, 4.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14034, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 119, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (134.82, 74.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.87, 96.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.956 CPU sec, 4.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13956, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 144, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (142.67, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.40, 93.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.412 CPU sec, 4.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14025, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (127.91, 91.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.20, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.192 CPU sec, 5.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13850, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 134, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (164.64, 110.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.92, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.940 CPU sec, 3.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13935, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 101, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (132.64, 94.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.57, 93.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.496 CPU sec, 4.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14092, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 165, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (151.17, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.22, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.358 CPU sec, 4.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13938, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (152.71, 98.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.43, 92.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.078 CPU sec, 4.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14052, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 97, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (120.50, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.84, 93.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.466 CPU sec, 4.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13732, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 141, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (133.30, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.77, 91.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.203 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13751, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 91, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (101.44, 55.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.06, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.329 CPU sec, 4.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13750, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 182, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (167.93, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (194.80, 92.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.236 CPU sec, 4.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13764, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 112, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (139.23, 79.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.06, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.704 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13893, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 140, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (138.50, 72.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (194.73, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.397 CPU sec, 4.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13748, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (135.35, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.79, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.086 CPU sec, 4.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13825, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 120, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (137.27, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.71, 92.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.664 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14037, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 131, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (134.56, 68.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.76, 90.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.422 CPU sec, 4.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13913, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (134.25, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.95, 92.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.606 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13945, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 154, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (175.81, 111.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.22, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.434 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13989, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 99, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (104.29, 47.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.98, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.353 CPU sec, 4.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13916, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 152, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (151.64, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.74, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.175 CPU sec, 3.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13951, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 109, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (118.47, 64.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.24, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.075 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13777, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (155.96, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.00, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.757 CPU sec, 4.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14049, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (125.71, 58.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.69, 93.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.045 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14031, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 111, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (146.74, 91.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.56, 94.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.619 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13739, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 128, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (128.84, 59.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.16, 94.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.383 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13941, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 179, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (176.35, 107.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.50, 88.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.854 CPU sec, 4.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13822, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 92, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (111.42, 64.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.12, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.605 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13861, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 102, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (115.30, 74.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (193.37, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.566 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13806, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 100, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (116.45, 56.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.98, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.626 CPU sec, 4.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13803, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 126, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (140.78, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (194.33, 91.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.784 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13676, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 92, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (115.86, 56.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.44, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.821 CPU sec, 4.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13733, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (89.27, 61.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (192.67, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.154 CPU sec, 3.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13769, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 150, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (151.14, 84.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.00, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.842 CPU sec, 5.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13602, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 129, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (149.17, 91.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.56, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.359 CPU sec, 6.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13580, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (152.52, 99.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.17, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.255 CPU sec, 4.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13278, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 95, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (105.57, 63.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.93, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.616 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13386, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 97, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (105.78, 47.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.93, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.274 CPU sec, 5.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13646, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 101, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (128.00, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.97, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.762 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13933, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 166, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (162.00, 102.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.15, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.682 CPU sec, 4.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13972, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 173, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (211.74, 175.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.14, 99.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.049 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14113, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 136, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (156.24, 96.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.61, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.754 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13958, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (164.87, 78.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (199.05, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.835 CPU sec, 4.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14289, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 131, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (163.45, 99.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.50, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.120 CPU sec, 5.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14079, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (123.53, 63.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (197.45, 92.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.510 CPU sec, 4.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14033, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 153, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (164.67, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.23, 95.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.826 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13986, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 134, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (168.67, 107.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.10, 94.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.831 CPU sec, 4.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14004, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 105, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (132.48, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (197.95, 94.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.463 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14027, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 150, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (142.85, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.51, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.918 CPU sec, 4.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14058, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 269, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (221.07, 143.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.38, 94.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.359 CPU sec, 4.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13942, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (118.83, 57.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.37, 95.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.421 CPU sec, 4.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13989, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 91, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (117.74, 77.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.66, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.385 CPU sec, 6.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14128, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 159, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (160.50, 102.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.30, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.636 CPU sec, 4.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14176, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 108, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (129.78, 71.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.09, 94.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.092 CPU sec, 4.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13925, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 132, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (177.95, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.04, 99.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.625 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13933, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 99, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (117.80, 73.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (201.54, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.411 CPU sec, 4.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13880, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (131.45, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.90, 96.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.004 CPU sec, 5.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13732, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 147, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (121.00, 52.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (201.51, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.982 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13974, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 154, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (151.42, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.02, 95.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.834 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13928, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 102, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (125.42, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.45, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.749 CPU sec, 6.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14042, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 108, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (142.50, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (201.49, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.471 CPU sec, 5.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13858, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (135.78, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.80, 96.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.105 CPU sec, 6.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14062, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 159, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.90, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.14, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.358 CPU sec, 4.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13970, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (188.00, 101.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.00, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.274 CPU sec, 4.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14045, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 164, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (172.76, 85.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (201.98, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.492 CPU sec, 4.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14069, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 156, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (160.24, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.16, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.622 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13924, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 108, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (164.38, 109.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.67, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.052 CPU sec, 3.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13849, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 86, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (108.21, 63.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.39, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.475 CPU sec, 6.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14095, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 100, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (134.96, 105.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.09, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.100 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13856, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 116, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (139.93, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.61, 92.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.945 CPU sec, 4.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14108, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 129, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (160.19, 101.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.48, 90.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.091 CPU sec, 4.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13747, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 121, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (121.61, 71.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.57, 92.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.200 CPU sec, 5.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13766, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 117, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (142.17, 80.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.14, 91.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.144 CPU sec, 4.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13972, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 121, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (164.61, 102.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.58, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.257 CPU sec, 4.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13785, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 157, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (136.73, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.78, 89.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.362 CPU sec, 6.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13829, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 88, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (116.92, 68.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.39, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.361 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13665, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 91, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (116.28, 83.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.06, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.124 CPU sec, 5.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13495, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 110, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (138.74, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.78, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.244 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13547, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (166.47, 111.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.13, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.643 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13350, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 95, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (145.43, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.09, 88.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.725 CPU sec, 5.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13421, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 150, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (161.73, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (194.80, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.244 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13308, 1, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (158.43, 111.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (194.98, 90.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.810 CPU sec, 5.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13992, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 94, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (134.17, 102.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.41, 99.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.643 CPU sec, 4.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13952, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (157.24, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.42, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.411 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14188, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 92, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (114.31, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.18, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.775 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14056, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 118, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (137.23, 83.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.66, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.050 CPU sec, 5.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13907, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 131, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (146.44, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.67, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.567 CPU sec, 4.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14094, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 117, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (152.54, 98.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.46, 98.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.572 CPU sec, 3.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14053, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (155.24, 110.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.35, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.557 CPU sec, 4.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13873, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 128, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (136.33, 70.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.98, 95.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.137 CPU sec, 4.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14107, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 100, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (140.65, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.80, 99.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.791 CPU sec, 4.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13999, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (111.61, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.63, 93.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.385 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13902, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 136, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (128.76, 64.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (200.84, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.652 CPU sec, 4.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13753, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 96, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (116.00, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (196.88, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.104 CPU sec, 4.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14117, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 97, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (92.62, 42.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.16, 96.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.393 CPU sec, 4.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14119, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 106, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (119.35, 55.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.40, 97.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.499 CPU sec, 4.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13994, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 128, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (135.58, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.47, 95.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.735 CPU sec, 4.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13989, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 160, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (153.27, 70.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.03, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.276 CPU sec, 5.237 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13958, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 103, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (120.83, 51.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.39, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.488 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13990, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (116.62, 54.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.29, 95.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.971 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13940, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 123, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (134.67, 86.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (206.01, 101.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.733 CPU sec, 4.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14011, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 111, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (126.75, 45.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.48, 98.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.687 CPU sec, 5.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14168, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 141, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (164.77, 102.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (201.76, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.447 CPU sec, 4.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13867, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 171, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (128.35, 70.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.12, 98.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.483 CPU sec, 4.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13887, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 93, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (105.31, 55.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.26, 98.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.088 CPU sec, 5.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14078, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 103, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (124.68, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (204.69, 100.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.795 CPU sec, 4.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13996, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (116.24, 65.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (203.07, 98.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.279 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14065, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (123.96, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (204.48, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.300 CPU sec, 4.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13892, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (141.18, 87.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.22, 98.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.805 CPU sec, 5.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13950, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 130, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (139.88, 86.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (203.11, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.207 CPU sec, 5.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13939, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 122, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (126.95, 65.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.01, 98.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.539 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14060, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 131, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (137.89, 89.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (200.66, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.103 CPU sec, 4.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13837, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (164.19, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.88, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.920 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14022, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 100, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (108.95, 60.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.21, 91.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.839 CPU sec, 4.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13908, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 125, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (138.71, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.90, 92.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.370 CPU sec, 3.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14065, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 133, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (151.43, 106.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.00, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.516 CPU sec, 4.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13890, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (137.81, 59.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.94, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.866 CPU sec, 4.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13878, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 100, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (141.58, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.48, 91.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.171 CPU sec, 4.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13893, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 119, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (122.24, 59.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.84, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.258 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13738, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (137.00, 86.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.87, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.499 CPU sec, 4.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13773, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 138, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (137.50, 60.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.58, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.280 CPU sec, 5.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13640, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 156, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (152.57, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.05, 89.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.959 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13559, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 86, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (151.26, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.41, 87.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.808 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13614, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 128, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (119.90, 58.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.69, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.769 CPU sec, 5.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13401, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 124, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (143.90, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.85, 91.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.176 CPU sec, 5.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13743, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 93, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (115.17, 75.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.97, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.894 CPU sec, 4.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14006, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 148, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (79, 229) [M::mem_pestat] mean and std.dev: (138.00, 37.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.92, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.226 CPU sec, 4.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14124, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 96, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (112.82, 79.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.47, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.900 CPU sec, 4.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13982, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (115.50, 70.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.29, 95.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.900 CPU sec, 4.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14050, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 108, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (139.55, 87.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (203.16, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.225 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13915, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 150, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (151.76, 85.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (200.02, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.274 CPU sec, 3.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14032, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 102, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (127.82, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.90, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.194 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13883, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 146, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (149.00, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (202.16, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.197 CPU sec, 4.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14209, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 131, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (132.95, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.89, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.638 CPU sec, 4.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14029, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 176, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (166.72, 90.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.54, 94.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.202 CPU sec, 4.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13985, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 132, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (139.81, 68.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.66, 95.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.064 CPU sec, 4.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14137, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 152, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (133.50, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.42, 93.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.694 CPU sec, 5.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13988, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (137.77, 78.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (199.94, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.700 CPU sec, 4.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14101, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 93, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (152.54, 110.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.46, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.289 CPU sec, 4.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14082, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 106, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (123.47, 67.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.58, 96.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.053 CPU sec, 3.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13975, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (95.72, 43.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.29, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.847 CPU sec, 4.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14006, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 130, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (150.37, 84.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.32, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.246 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14034, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (173.68, 108.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.37, 99.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.708 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13990, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 105, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (126.63, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.63, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.598 CPU sec, 4.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14034, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (126.06, 76.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.04, 96.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.972 CPU sec, 5.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14037, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (110.77, 55.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.69, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.792 CPU sec, 4.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14029, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (140.23, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (202.31, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.287 CPU sec, 5.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14007, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (158.59, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.61, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.200 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13983, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 130, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (102.77, 49.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (200.33, 96.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.665 CPU sec, 6.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14044, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 151, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (183.95, 97.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.39, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.289 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13933, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 147, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (156.89, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.75, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.386 CPU sec, 4.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14101, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 143, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (140.19, 105.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (202.54, 98.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.103 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14019, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 106, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (160.74, 129.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.81, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.208 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14176, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 130, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (153.86, 108.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.41, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.366 CPU sec, 4.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14110, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 119, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (150.25, 84.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.49, 98.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.165 CPU sec, 4.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13789, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 127, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (145.00, 68.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.86, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.808 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13774, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 91, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (96.42, 49.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.90, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.555 CPU sec, 5.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13824, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 145, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (163.92, 87.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.79, 88.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.839 CPU sec, 4.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13981, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 115, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (143.06, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.02, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.763 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13903, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 153, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (153.86, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (191.33, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.955 CPU sec, 4.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13861, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 137, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (151.11, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (190.52, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.462 CPU sec, 5.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13779, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 120, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (143.47, 98.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (189.49, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.787 CPU sec, 4.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13863, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 101, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (136.95, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.98, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.595 CPU sec, 4.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13705, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 137, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (113.78, 48.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 167, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.07, 84.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.181 CPU sec, 5.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13505, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 89, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (110.80, 81.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.80, 85.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.120 CPU sec, 4.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13401, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (156.21, 81.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.53, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.440 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13360, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 160, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (151.19, 64.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 165, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (186.08, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.779 CPU sec, 5.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13458, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 148, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (162.29, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 167, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.21, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.989 CPU sec, 4.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13360, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 124, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (141.70, 98.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.41, 86.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.241 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14007, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 115, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (135.04, 81.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (198.25, 94.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.082 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13914, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 116, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (149.40, 94.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.76, 93.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.753 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13998, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 79, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (105.73, 55.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.34, 96.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.183 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13830, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 108, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (135.32, 68.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.97, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.771 CPU sec, 4.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13989, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 145, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (161.74, 87.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.27, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.070 CPU sec, 4.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13967, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 119, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (117.60, 76.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (198.80, 96.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.427 CPU sec, 5.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13942, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 198, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (164.38, 98.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.35, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.845 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13932, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 128, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (161.17, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.64, 95.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.390 CPU sec, 6.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13975, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 141, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (155.68, 94.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.94, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.850 CPU sec, 4.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13989, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (117.07, 58.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.68, 90.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.718 CPU sec, 4.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14075, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 114, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (113.87, 55.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.20, 92.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.153 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14053, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 124, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (135.05, 64.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.98, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.580 CPU sec, 4.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14017, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 140, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (153.00, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.11, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.481 CPU sec, 5.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13953, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 128, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (138.68, 89.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (195.51, 92.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.889 CPU sec, 6.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14126, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (163.58, 82.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.03, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.888 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13817, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (124.58, 61.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.45, 95.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.130 CPU sec, 4.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14030, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 93, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (101.81, 55.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.03, 96.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.757 CPU sec, 4.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13785, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 123, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (131.95, 70.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.65, 93.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.500 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13985, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (114.43, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.59, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.042 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13916, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 109, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (122.50, 59.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.31, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.774 CPU sec, 5.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14013, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (154.75, 71.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.41, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.003 CPU sec, 4.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14138, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (154.45, 83.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.82, 96.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.002 CPU sec, 3.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13844, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 130, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (136.32, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.79, 94.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.036 CPU sec, 4.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13894, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 108, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (131.00, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (200.40, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.004 CPU sec, 3.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13841, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 156, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (160.42, 66.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.89, 96.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.323 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14068, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 102, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (121.67, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.89, 94.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.392 CPU sec, 4.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13994, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (133.75, 74.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (199.83, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.964 CPU sec, 3.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13926, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (169.45, 77.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (198.72, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.428 CPU sec, 4.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14033, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (125.00, 47.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (201.89, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.852 CPU sec, 6.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14066, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 156, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (147.62, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.26, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.687 CPU sec, 5.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13976, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 209, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (189.67, 65.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.48, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.749 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13905, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (127.09, 79.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.58, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.606 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13907, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (125.76, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (194.42, 90.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.516 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13819, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 100, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (111.41, 48.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (192.71, 89.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.810 CPU sec, 4.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13838, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 99, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (91.07, 58.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.78, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.289 CPU sec, 4.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13928, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 129, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (156.35, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.95, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.903 CPU sec, 4.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13627, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 132, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (141.00, 81.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.16, 86.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.444 CPU sec, 3.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13752, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 124, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (124.00, 53.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (186.32, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.351 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13550, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (132.47, 75.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.75, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.290 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13465, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 166, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (155.81, 103.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (185.84, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.243 CPU sec, 4.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13428, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 115, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (131.00, 83.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.84, 83.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.446 CPU sec, 4.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13298, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 88, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (113.72, 65.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 165, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (185.85, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.677 CPU sec, 4.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13275, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (139.91, 76.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (187.78, 83.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.348 CPU sec, 4.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14001, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 97, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (107.40, 69.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (199.45, 95.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.992 CPU sec, 3.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14053, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 169, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (163.55, 86.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (200.01, 96.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.178 CPU sec, 4.994 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14113, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 90, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (81.35, 41.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (201.19, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.251 CPU sec, 5.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14039, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 116, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (170.37, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (197.36, 93.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.352 CPU sec, 4.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13985, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 109, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (126.09, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.90, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.338 CPU sec, 4.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13987, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 112, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (139.55, 77.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (198.70, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.532 CPU sec, 5.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14133, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 157, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (149.30, 60.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (197.24, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.233 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13920, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 113, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (117.92, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.72, 94.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.753 CPU sec, 4.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14122, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 129, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (167.94, 85.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.66, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.461 CPU sec, 4.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13928, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (109.35, 55.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.40, 96.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.504 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13994, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (156.35, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.00, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.775 CPU sec, 4.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14043, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 132, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (150.26, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.06, 92.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.462 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13972, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 113, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (125.05, 63.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.90, 93.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.101 CPU sec, 6.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13954, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 105, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (99.78, 59.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.77, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.765 CPU sec, 4.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13969, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 108, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (119.79, 50.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (197.08, 92.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.896 CPU sec, 4.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13969, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (128.28, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (197.57, 94.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.693 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14019, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 152, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (137.41, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.19, 94.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.864 CPU sec, 4.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13868, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (107.20, 53.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.85, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.571 CPU sec, 4.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13937, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 127, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (146.94, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.65, 94.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.994 CPU sec, 4.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13961, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 159, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (191.67, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (199.59, 96.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.313 CPU sec, 5.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14007, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 124, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (159.92, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.29, 97.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.522 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13980, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 105, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (118.19, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.63, 96.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.791 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14026, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (102.81, 54.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.56, 97.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.417 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14055, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (146.09, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.40, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.449 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13840, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 174, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (172.36, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.80, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.158 CPU sec, 4.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14013, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 111, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 227) [M::mem_pestat] mean and std.dev: (115.58, 34.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.67, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.344 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13951, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 96, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (101.72, 53.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.91, 96.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.446 CPU sec, 4.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13878, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 132, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (185.91, 133.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.24, 96.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.414 CPU sec, 3.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13991, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 137, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (162.20, 114.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.89, 95.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.805 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13879, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 160, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (156.50, 93.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.32, 96.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.866 CPU sec, 4.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13990, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (142.92, 104.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.40, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.609 CPU sec, 5.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14065, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 89, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (125.64, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.16, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.911 CPU sec, 4.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13848, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 138, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (147.15, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.52, 89.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.858 CPU sec, 5.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13756, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (145.59, 80.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.71, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.618 CPU sec, 4.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13797, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (114.00, 53.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.81, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.279 CPU sec, 4.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13908, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 146, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (152.61, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (192.25, 88.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.028 CPU sec, 4.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13793, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (147.68, 91.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.27, 88.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.823 CPU sec, 4.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13716, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 115, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (155.77, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (190.15, 86.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.813 CPU sec, 4.785 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13757, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 170, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (171.96, 96.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (190.24, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.162 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13556, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 136, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (149.67, 86.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (191.86, 88.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.651 CPU sec, 4.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13514, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 126, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (136.24, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (190.12, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.383 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13536, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 127, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (135.72, 70.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.62, 86.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.179 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13369, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 97, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (97.86, 54.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.10, 84.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.614 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13488, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (127.41, 93.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.26, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.697 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13941, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 135, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (160.81, 109.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.22, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.323 CPU sec, 4.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13906, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 131, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (144.00, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (199.66, 95.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.157 CPU sec, 4.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14016, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (142.86, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (197.41, 94.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.582 CPU sec, 4.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14126, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 114, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (126.70, 44.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (200.03, 95.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.369 CPU sec, 4.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13860, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 144, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (149.04, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.59, 95.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.631 CPU sec, 4.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14020, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 117, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (130.00, 70.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (200.63, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.293 CPU sec, 4.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14035, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 197, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (168.88, 94.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.24, 93.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.363 CPU sec, 4.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14063, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 92, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (131.53, 104.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.81, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.963 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14051, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 88, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (119.24, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.00, 94.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.724 CPU sec, 4.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14075, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 107, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (121.15, 62.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.07, 92.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.269 CPU sec, 4.609 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14082, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 154, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (166.36, 78.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.70, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.254 CPU sec, 4.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14063, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 145, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (151.91, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.54, 93.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.681 CPU sec, 4.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14042, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 135, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (159.42, 91.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.92, 92.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.128 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14060, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 132, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (151.00, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.91, 91.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.212 CPU sec, 4.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13995, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 133, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (163.85, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.99, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.263 CPU sec, 4.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14032, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 115, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (117.92, 64.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.80, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.845 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14061, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 197, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (173.21, 103.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.29, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.057 CPU sec, 4.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13898, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 144, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (141.80, 70.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (199.57, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.185 CPU sec, 5.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13890, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (121.88, 52.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (199.03, 95.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.661 CPU sec, 4.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13984, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 150, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (146.38, 96.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (198.72, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.367 CPU sec, 5.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13902, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (154.79, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.67, 93.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.662 CPU sec, 4.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13979, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 89, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (124.00, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (197.90, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.173 CPU sec, 4.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13748, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (136.00, 67.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.16, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.924 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13842, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 203, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (186.59, 92.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (198.47, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.243 CPU sec, 5.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13954, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (138.71, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (200.07, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.317 CPU sec, 4.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13949, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 114, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (153.82, 96.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (196.09, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.585 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13914, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 84, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (81.55, 24.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.32, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.592 CPU sec, 4.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13935, 2, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (157.50, 83.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (197.82, 94.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.081 CPU sec, 5.499 real sec [M::process] read 23560 sequences (3557560 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13866, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (179.24, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.83, 95.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.129 CPU sec, 6.254 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 5034, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (130.40, 44.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 176, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (198.66, 93.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 23560 reads in 47.848 CPU sec, 2.352 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21174\tSM:UG10S2P419\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21174_HLKNYBGXX_UG10S2P419_R1.fastq.gz 5347_7332_21174_HLKNYBGXX_UG10S2P419_R2.fastq.gz [main] Real time: 5117.909 sec; CPU: 128768.983 sec Finished alignement of UG10S2P419 at Wed Apr 3 11:34:44 2019 Sorting BAM file started at Wed Apr 3 11:34:44 2019 samtools sort -@ 30 -m 4G UG10S2P419_unsorted.bam -o UG10S2P419.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 11:34:44 2019 rm UG10S2P419_unsorted.bam rm 5347_7332_21174_HLKNYBGXX_UG10S2P419_R1.fastq.gz rm 5347_7332_21174_HLKNYBGXX_UG10S2P419_R2.fastq.gz samtools index UG10S2P419.bam Finished BAM file generation of the sample - UG10S2P419 Processing fastq files for the sample - UG10S2P415 Reading 5347_7332_21175_HLKNYBGXX_UG10S2P415_R1.fastq.gz 5347_7332_21175_HLKNYBGXX_UG10S2P415_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21175\tSM:UG10S2P415\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21175_HLKNYBGXX_UG10S2P415_R1.fastq.gz 5347_7332_21175_HLKNYBGXX_UG10S2P415_R2.fastq.gz | samtools view -Sb1 - > UG10S2P415_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11965, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 180, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (173.50, 119.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 274, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (291.58, 135.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.705 CPU sec, 7.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12336, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 142, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (165.73, 105.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (289.56, 133.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.395 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12456, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 183, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (182.31, 105.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 279, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (293.86, 134.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.474 CPU sec, 7.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12601, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 135, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (162.32, 101.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 280, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (294.44, 135.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.368 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12576, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 172, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (220.86, 151.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (293.37, 135.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.253 CPU sec, 5.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12794, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 178, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (192.48, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 279, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (293.45, 135.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.182 CPU sec, 6.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12968, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 158, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (197.73, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (291.43, 134.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.165 CPU sec, 6.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12663, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 176, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (209.52, 115.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 276, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (291.82, 134.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.235 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12730, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 100, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (109.32, 65.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (289.64, 133.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.770 CPU sec, 5.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12758, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 229, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (249.48, 141.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (288.18, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.275 CPU sec, 5.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12675, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 179, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (202.50, 126.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (285.87, 131.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.612 CPU sec, 6.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12728, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 201, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (218.36, 110.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (281.67, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.431 CPU sec, 6.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12768, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 140, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (167.22, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (281.23, 129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.708 CPU sec, 5.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12592, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 215, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (224.95, 122.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (280.01, 129.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.470 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12506, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (166.12, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (278.53, 128.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.472 CPU sec, 6.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12214, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 139, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (160.36, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (288.19, 132.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.266 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12120, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 121, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (146.67, 89.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 275, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (292.94, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.197 CPU sec, 6.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12358, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 188, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (190.90, 103.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (291.59, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.563 CPU sec, 6.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12516, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 175, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (192.64, 112.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (289.21, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.948 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12729, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 202, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (222.36, 123.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (293.23, 134.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.716 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12795, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 192, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (213.17, 150.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (292.81, 135.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.007 CPU sec, 6.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12631, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 219, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (192.90, 107.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (290.19, 133.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.339 CPU sec, 5.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12694, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 145, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (184.24, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (293.27, 133.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.791 CPU sec, 5.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12559, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 217, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (222.09, 131.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (290.18, 132.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.118 CPU sec, 6.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12815, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (177.41, 67.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (288.42, 133.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.956 CPU sec, 5.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12744, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (165.76, 99.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (287.53, 131.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.611 CPU sec, 6.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12758, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 175, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (195.79, 124.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.13, 130.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.344 CPU sec, 5.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12682, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 162, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (165.38, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (279.77, 129.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.831 CPU sec, 4.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12703, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 153, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (168.60, 79.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (279.05, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.712 CPU sec, 5.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12559, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 161, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (187.73, 106.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (276.71, 128.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.739 CPU sec, 4.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12452, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 166, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (181.67, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (277.27, 128.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.180 CPU sec, 5.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12187, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 212, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (190.28, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (291.92, 133.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.146 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12221, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 190, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (262.75, 209.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 277, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (290.21, 132.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.711 CPU sec, 4.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12329, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (238.22, 162.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (289.11, 133.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.515 CPU sec, 4.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12533, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 162, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (186.67, 116.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.80, 134.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.051 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12720, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 151, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (154.27, 94.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (288.50, 133.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.011 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12466, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (177.06, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (288.27, 134.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.462 CPU sec, 5.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12683, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 142, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (162.60, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (289.94, 134.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.471 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12565, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 186, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (231.33, 127.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (289.89, 133.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.857 CPU sec, 8.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12459, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 111, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (146.79, 91.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.26, 132.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.123 CPU sec, 6.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12685, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 144, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (166.62, 104.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (283.92, 131.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.331 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12597, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 146, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (137.71, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (284.34, 131.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.028 CPU sec, 6.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12532, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 156, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (201.14, 143.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (279.42, 129.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.118 CPU sec, 5.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12664, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (202.48, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (279.50, 129.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.089 CPU sec, 5.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12402, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 147, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (215.48, 132.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (276.71, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.159 CPU sec, 6.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12322, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 170, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (208.97, 151.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (276.78, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.628 CPU sec, 6.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12388, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 137, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (194.73, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (279.03, 129.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.098 CPU sec, 6.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12297, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 196, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (202.25, 118.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (283.95, 130.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.350 CPU sec, 6.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12628, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 165, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (192.90, 128.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (286.01, 131.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.438 CPU sec, 5.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12569, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (136.39, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (287.55, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.435 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12615, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 282, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (277.71, 149.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (289.96, 134.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.172 CPU sec, 5.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12647, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 242, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (209.21, 133.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (286.21, 131.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.385 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12774, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 242, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (213.67, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.08, 132.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.543 CPU sec, 6.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12631, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (177.24, 104.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 271, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (286.93, 132.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.041 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12810, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (202.04, 88.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (288.31, 132.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.947 CPU sec, 6.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12627, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 187, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (186.09, 104.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (282.54, 129.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.385 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12462, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 150, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (173.85, 106.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (282.79, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.498 CPU sec, 5.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12650, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (187.08, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (276.25, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.077 CPU sec, 6.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12721, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 171, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (186.32, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (278.12, 128.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.915 CPU sec, 6.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12537, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 178, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 336) [M::mem_pestat] mean and std.dev: (185.00, 61.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (274.98, 126.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.610 CPU sec, 5.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12471, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 225, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (212.24, 130.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (276.15, 127.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.871 CPU sec, 5.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12262, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (173.78, 99.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 257, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (275.93, 126.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.126 CPU sec, 5.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11957, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (185.28, 101.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (279.86, 129.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.412 CPU sec, 6.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12262, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (154.67, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (283.19, 128.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.669 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12489, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 111, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (121.19, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 263, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.00, 130.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.423 CPU sec, 5.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12594, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (177.24, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (287.81, 133.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.890 CPU sec, 6.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12834, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 203, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (186.35, 91.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (287.27, 132.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.271 CPU sec, 6.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12675, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 167, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (177.27, 108.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (286.63, 130.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.401 CPU sec, 5.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12820, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 166, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (196.10, 145.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (286.01, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.590 CPU sec, 6.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12660, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 156, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (169.25, 100.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (287.22, 132.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.864 CPU sec, 7.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12848, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 153, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (190.18, 132.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 268, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (285.25, 132.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.883 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12607, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 222, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (191.57, 99.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (283.66, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.076 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12684, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 172, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 323) [M::mem_pestat] mean and std.dev: (166.00, 58.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (278.22, 128.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.772 CPU sec, 5.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12539, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (214.76, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (276.01, 127.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.835 CPU sec, 7.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12395, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 169, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (177.16, 107.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 254, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (276.17, 126.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.515 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12501, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 163, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (173.44, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (274.44, 127.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.327 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12395, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 261, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (223.45, 115.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (275.81, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.504 CPU sec, 6.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11996, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 207, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (207.17, 107.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (280.50, 128.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.118 CPU sec, 6.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12156, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 201, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (205.31, 111.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (281.04, 129.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.123 CPU sec, 6.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12432, 9, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 153, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (159.96, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (280.98, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.609 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12357, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (202.21, 116.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (283.25, 131.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.576 CPU sec, 6.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12491, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 218, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (212.21, 109.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (285.41, 132.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.590 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12475, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 136, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (152.68, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (285.67, 131.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.603 CPU sec, 5.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12439, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 181, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (217.13, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (286.73, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.041 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12489, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 156, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (152.70, 59.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 264, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (282.64, 129.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.181 CPU sec, 5.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12423, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 161, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (195.26, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (280.55, 129.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.402 CPU sec, 6.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12606, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 150, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (177.90, 116.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (280.20, 130.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.884 CPU sec, 6.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12535, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 143, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (164.20, 107.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (278.66, 127.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.498 CPU sec, 5.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12404, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 171, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (190.50, 106.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (274.36, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.292 CPU sec, 6.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12286, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 148, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (155.68, 81.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (272.86, 126.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.423 CPU sec, 6.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12487, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (156.41, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.51, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.920 CPU sec, 6.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12552, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 276, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (225.29, 107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.00, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.780 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12492, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 198, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (206.20, 121.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 279, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (292.49, 134.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.567 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12627, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 167, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (160.60, 85.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 279, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (294.46, 134.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.272 CPU sec, 6.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12519, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 237, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (245.24, 142.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (289.49, 132.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.615 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12737, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 175, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (164.90, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 274, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.02, 133.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.211 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12511, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 165, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (166.62, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (291.23, 132.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.772 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12669, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 126, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (153.46, 102.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (291.01, 134.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.027 CPU sec, 6.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12733, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 165, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (211.29, 110.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (291.31, 133.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.955 CPU sec, 5.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12606, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (172.42, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (291.54, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.993 CPU sec, 7.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12599, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 190, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (201.76, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (288.79, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.040 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12519, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 143, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (165.46, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (287.09, 131.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.046 CPU sec, 6.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12770, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 257, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (230.03, 136.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (286.24, 132.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.772 CPU sec, 5.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12466, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 163, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (178.79, 108.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 260, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.20, 130.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.092 CPU sec, 6.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12149, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 251, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (243.42, 131.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (277.95, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.737 CPU sec, 6.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12064, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 199, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (224.37, 160.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.38, 126.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.452 CPU sec, 6.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11677, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 161, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (188.81, 104.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (271.39, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.072 CPU sec, 6.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11804, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 170, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (167.56, 110.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 263, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.37, 129.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.988 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12437, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 184, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (229.28, 140.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 283, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (296.38, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.147 CPU sec, 7.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12681, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 152, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (186.21, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (297.18, 134.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.519 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12587, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 143, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (183.59, 127.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 284, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (297.80, 135.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.930 CPU sec, 5.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12625, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 196, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (199.29, 130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (294.06, 134.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.837 CPU sec, 5.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12585, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 157, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (210.31, 125.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (295.19, 134.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.492 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12530, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 219, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (261.00, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 281, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (295.66, 135.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.849 CPU sec, 6.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12575, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 145, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (181.12, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (293.24, 135.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.203 CPU sec, 6.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12699, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 255, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (235.91, 110.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (292.46, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.316 CPU sec, 7.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12612, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 224, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (222.73, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.41, 132.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.699 CPU sec, 6.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12687, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 210, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (227.53, 146.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (290.21, 134.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.413 CPU sec, 7.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12706, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 224, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (259.68, 129.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (287.26, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.082 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12271, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 174, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (180.26, 114.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (277.55, 127.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.550 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12188, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (173.50, 94.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (274.61, 125.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.030 CPU sec, 5.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12040, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 128, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (171.08, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.28, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.215 CPU sec, 6.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11570, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (153.32, 93.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (274.67, 126.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.760 CPU sec, 7.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12020, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 162, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (201.96, 119.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (292.81, 134.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.392 CPU sec, 6.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12675, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (153.33, 93.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 284, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (296.48, 135.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.610 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12627, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 223, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (190.46, 106.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 282, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (295.70, 135.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.816 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12546, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 210, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (236.59, 143.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (296.12, 135.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.500 CPU sec, 5.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12570, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 206, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (210.38, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (292.47, 134.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.995 CPU sec, 6.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12600, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (182.82, 106.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 284, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.52, 134.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.997 CPU sec, 6.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12624, 0, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 139, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (205.22, 127.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (292.74, 134.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.861 CPU sec, 5.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12468, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 254, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (238.68, 125.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 276, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (291.88, 134.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.162 CPU sec, 7.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12643, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 206, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (208.52, 122.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.06, 132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.804 CPU sec, 5.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12648, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (250.70, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (287.28, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.522 CPU sec, 6.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12639, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 187, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (165.88, 89.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (285.45, 131.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.986 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12410, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 125, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (173.78, 109.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (280.22, 129.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.390 CPU sec, 7.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12273, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 151, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (172.84, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (276.69, 128.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.018 CPU sec, 6.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12134, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (192.96, 115.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (271.46, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.782 CPU sec, 7.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11970, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (169.88, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.26, 124.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.397 CPU sec, 6.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11520, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 244, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (206.40, 104.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (279.47, 129.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.269 CPU sec, 7.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12534, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 175, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (233.63, 151.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (289.13, 132.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.690 CPU sec, 5.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12473, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 231, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (217.52, 129.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (286.77, 130.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.291 CPU sec, 5.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12435, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 139, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (175.67, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 269, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (286.45, 130.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.763 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12716, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (186.70, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.37, 132.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.156 CPU sec, 5.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12600, 1, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 195, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (202.84, 112.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (287.26, 130.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.325 CPU sec, 6.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12630, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 280, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (253.76, 165.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (286.24, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.352 CPU sec, 5.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12528, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 169, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (185.22, 100.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (289.60, 133.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.968 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12630, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (204.30, 86.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.11, 130.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.117 CPU sec, 6.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12563, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 306, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (288.93, 126.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (287.86, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.977 CPU sec, 5.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12355, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 142, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (162.97, 94.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (283.20, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.554 CPU sec, 6.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12392, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 196, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (189.55, 102.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (279.07, 127.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.393 CPU sec, 5.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12368, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (166.27, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.16, 126.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.977 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12207, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 135, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (166.44, 130.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (270.25, 123.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.549 CPU sec, 6.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11940, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 145, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (168.45, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 249, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.60, 120.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.243 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11630, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 135, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (139.30, 85.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.51, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.512 CPU sec, 6.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12185, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 203, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (198.07, 102.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (284.63, 130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.869 CPU sec, 6.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12549, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (210.29, 105.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (290.66, 132.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.718 CPU sec, 6.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12605, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (190.62, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (291.28, 134.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.500 CPU sec, 7.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12556, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 202, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (207.05, 108.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 272, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (289.28, 134.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.595 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12732, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 180, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (198.46, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.37, 132.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.499 CPU sec, 6.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12588, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 173, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (196.32, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (289.73, 132.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.843 CPU sec, 5.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12687, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 154, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (176.64, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 274, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (289.70, 133.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.914 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12711, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 193, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (187.05, 104.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 274, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (290.57, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.353 CPU sec, 5.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12591, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 221, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (215.40, 114.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (289.50, 133.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.036 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12603, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 157, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (182.95, 109.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (284.15, 131.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.510 CPU sec, 7.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12467, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 182, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (197.78, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (281.50, 130.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.643 CPU sec, 6.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12369, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 138, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (185.80, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (275.57, 127.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.864 CPU sec, 5.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12416, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 211, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (203.35, 163.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.98, 125.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.658 CPU sec, 6.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12215, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 146, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (168.00, 84.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.09, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.722 CPU sec, 6.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11561, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (154.05, 79.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 244, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.96, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.617 CPU sec, 6.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11856, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 141, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (195.12, 131.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.38, 129.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.732 CPU sec, 6.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12619, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 143, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (170.36, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (292.95, 134.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.149 CPU sec, 7.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12674, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 211, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (199.83, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (288.70, 131.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.316 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12608, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 159, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (159.47, 99.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (288.16, 133.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.027 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12635, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 225, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (228.00, 144.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (290.83, 133.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.120 CPU sec, 5.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12566, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (203.18, 140.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (289.43, 133.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.271 CPU sec, 6.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12683, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 232, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (222.29, 130.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (289.53, 133.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.213 CPU sec, 7.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12591, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 166, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (182.24, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (288.50, 130.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.008 CPU sec, 6.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12661, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 135, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (189.29, 127.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (286.65, 131.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.527 CPU sec, 5.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12557, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 175, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (209.76, 121.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (283.19, 130.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.468 CPU sec, 6.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12635, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 138, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (162.90, 68.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (281.97, 129.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.645 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12330, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 135, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (151.29, 91.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (277.04, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.788 CPU sec, 6.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12301, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 219, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (204.57, 107.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (272.85, 125.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.437 CPU sec, 5.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12151, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 143, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (160.68, 89.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (268.68, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.544 CPU sec, 5.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11758, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 183, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (202.04, 107.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (265.10, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.391 CPU sec, 5.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11492, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 265, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (218.47, 100.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (268.76, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.277 CPU sec, 6.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12677, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 233, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (232.10, 171.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (279.48, 128.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.490 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12326, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 154, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (169.05, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 256, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (276.07, 126.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.705 CPU sec, 6.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 12370, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 229, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (228.37, 126.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (276.60, 128.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.531 CPU sec, 5.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12459, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (186.68, 111.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (274.85, 126.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.154 CPU sec, 5.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12648, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 221, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (227.09, 142.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (290.62, 134.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.159 CPU sec, 5.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12795, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 138, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (157.17, 94.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.13, 133.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.644 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12647, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 146, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (187.55, 111.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (288.07, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.142 CPU sec, 6.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12579, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 156, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (180.73, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (286.25, 131.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.586 CPU sec, 5.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12615, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 242, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (204.63, 90.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 268, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (286.42, 132.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.766 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12214, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 152, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (212.55, 158.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.27, 132.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.818 CPU sec, 6.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12132, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 190, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (204.79, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (287.90, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.521 CPU sec, 5.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12492, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 224, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (223.58, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (287.71, 131.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.345 CPU sec, 5.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12493, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 236, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (246.89, 104.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (288.11, 131.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.826 CPU sec, 5.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12472, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 168, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (191.08, 122.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (289.11, 132.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.797 CPU sec, 5.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12669, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 182, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (169.00, 89.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 272, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (287.39, 132.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.346 CPU sec, 5.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12569, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 245, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (232.11, 113.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (278.18, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.621 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12449, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 114, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (174.88, 99.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (278.18, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.193 CPU sec, 6.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12594, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 141, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (168.62, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (274.98, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.900 CPU sec, 5.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12335, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 155, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (164.84, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (276.80, 127.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.228 CPU sec, 6.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12757, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 175, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (199.14, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 275, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (290.72, 134.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.643 CPU sec, 5.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12928, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 174, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (174.82, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (289.89, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.969 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12556, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 208, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (211.33, 99.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.17, 133.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.149 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12722, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 221, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (194.67, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (287.00, 131.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.166 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12593, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 203, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (202.67, 124.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (283.60, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.306 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12165, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 183, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (245.85, 152.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (290.57, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.570 CPU sec, 7.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12086, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 153, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (149.27, 81.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (287.43, 132.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.250 CPU sec, 7.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12258, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 153, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (175.86, 89.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (285.61, 131.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.747 CPU sec, 5.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12406, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 153, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (178.81, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (286.29, 132.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.804 CPU sec, 7.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12460, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 151, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (156.53, 82.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (286.23, 132.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.600 CPU sec, 5.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12732, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (196.88, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (285.33, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.174 CPU sec, 5.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12498, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 254, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (232.73, 129.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (276.78, 128.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.592 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12376, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (191.38, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (271.64, 126.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.485 CPU sec, 7.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12492, 10, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 130, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (123.92, 62.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (268.77, 124.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 2783, 7781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23203) [M::mem_pestat] mean and std.dev: (3634.60, 3544.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30914) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 160.770 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12408, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (166.00, 125.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (274.46, 127.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.375 CPU sec, 5.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12604, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 160, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (173.90, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (290.13, 134.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.626 CPU sec, 5.994 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12648, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 190, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (225.80, 151.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (288.88, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.580 CPU sec, 6.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12648, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 187, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (194.29, 103.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (286.95, 132.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.387 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12740, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 197, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (184.17, 65.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (280.65, 131.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.178 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12571, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 195, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (209.53, 168.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (279.38, 129.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.633 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12057, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 163, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (176.33, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (288.73, 133.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.904 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12237, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 210, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (224.26, 138.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (288.39, 131.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.966 CPU sec, 6.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12649, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 161, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (185.45, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (289.25, 133.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.915 CPU sec, 6.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12619, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 209, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (234.73, 129.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (289.27, 132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.806 CPU sec, 5.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12627, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (162.87, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (290.09, 134.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.343 CPU sec, 6.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12346, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 205, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (208.00, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (277.55, 128.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.452 CPU sec, 6.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12503, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 127, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (139.55, 86.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (274.37, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.847 CPU sec, 6.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12507, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 151, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (181.74, 135.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (276.27, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.430 CPU sec, 6.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12400, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (190.40, 84.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (272.83, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.808 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12476, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 147, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (137.00, 63.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.64, 127.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.172 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12623, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 226, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (207.43, 127.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 272, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (286.44, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.621 CPU sec, 5.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12623, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 191, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (222.00, 117.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (284.09, 131.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.817 CPU sec, 6.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12502, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 151, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (157.05, 80.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (284.32, 130.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.179 CPU sec, 5.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12686, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 145, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (162.55, 100.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (281.26, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.654 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12253, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 168, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (172.92, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (276.64, 128.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.420 CPU sec, 5.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12100, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 172, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (194.20, 109.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (274.46, 125.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.856 CPU sec, 5.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12287, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 196, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (200.90, 122.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (280.79, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.355 CPU sec, 5.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12363, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 163, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (180.39, 115.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (282.03, 129.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.894 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12545, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 159, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (198.32, 127.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (282.12, 130.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.438 CPU sec, 5.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12609, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 193, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (205.64, 107.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (283.39, 130.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.825 CPU sec, 5.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12431, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 203, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (197.87, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (275.66, 126.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.688 CPU sec, 5.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12506, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 166, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (130.15, 65.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.03, 126.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.679 CPU sec, 6.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12212, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 201, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (187.50, 109.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (273.85, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.885 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12270, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 175, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (203.17, 159.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (275.30, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.261 CPU sec, 5.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12550, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 162, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (180.19, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (283.30, 129.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.711 CPU sec, 5.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12699, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 189, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (200.48, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (284.38, 130.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.248 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12679, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 161, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (174.12, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 263, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (282.70, 130.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.353 CPU sec, 5.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12682, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (195.00, 105.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.56, 129.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.719 CPU sec, 5.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12640, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 207, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (183.77, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (279.95, 129.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.875 CPU sec, 5.522 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11935, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (140.79, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (279.63, 128.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.542 CPU sec, 6.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11970, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (168.90, 114.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (276.86, 127.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.110 CPU sec, 6.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12332, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 147, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (216.06, 173.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (279.76, 129.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.821 CPU sec, 6.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12614, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (175.79, 109.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (281.09, 130.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.121 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12772, 9, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 145, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (139.06, 62.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 265, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (282.46, 130.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.709 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12449, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 139, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (173.64, 114.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (277.08, 128.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.032 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12409, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 190, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (208.59, 110.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.13, 124.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.370 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12426, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 162, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (159.43, 91.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 251, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (271.78, 126.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.546 CPU sec, 6.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12343, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (163.22, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.41, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.115 CPU sec, 7.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12355, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 177, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (186.67, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (274.21, 126.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.971 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12678, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 148, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (165.26, 101.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (284.16, 130.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.364 CPU sec, 5.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12635, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 172, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (193.56, 120.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 261, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (281.88, 129.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.947 CPU sec, 5.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12721, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 130, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (170.10, 103.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (281.48, 130.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.592 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12413, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 220, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (206.03, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (279.59, 128.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.017 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12490, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 166, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (176.45, 100.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (278.13, 128.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.146 CPU sec, 6.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12185, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 133, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (155.18, 91.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (279.69, 128.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.376 CPU sec, 5.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12156, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (183.90, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (279.43, 129.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.370 CPU sec, 6.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12360, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 206, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (194.08, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (282.06, 130.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.321 CPU sec, 5.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12448, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (156.12, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (282.96, 131.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.119 CPU sec, 5.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12603, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 156, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (161.32, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (281.78, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.795 CPU sec, 5.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12362, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 186, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (188.40, 110.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.57, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.430 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12251, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 177, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (193.81, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.30, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.259 CPU sec, 5.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11919, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 162, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (190.00, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.02, 123.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.163 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11667, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 166, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (164.32, 85.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (272.73, 126.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.221 CPU sec, 6.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12074, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 254, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (201.17, 92.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (282.25, 130.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.961 CPU sec, 6.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12749, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 211, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (221.96, 161.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 276, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (289.78, 132.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.112 CPU sec, 6.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12727, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 240, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (234.81, 117.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 268, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (285.11, 132.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.987 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12631, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (203.54, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (284.70, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.273 CPU sec, 5.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12552, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 178, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (179.46, 93.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.33, 130.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.106 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12522, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 196, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (209.33, 129.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (282.38, 130.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.893 CPU sec, 5.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12597, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (201.25, 119.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (291.46, 132.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.118 CPU sec, 5.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12535, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 179, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (198.05, 132.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 275, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (290.58, 132.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.292 CPU sec, 5.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12525, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (191.21, 75.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (291.12, 132.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.645 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12508, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 257, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (219.26, 118.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (289.44, 133.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.001 CPU sec, 5.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12410, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 152, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (197.09, 134.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (285.53, 132.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.012 CPU sec, 5.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12291, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 178, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (191.41, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (275.40, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.305 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12091, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (257.22, 141.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.97, 126.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.687 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 11742, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 173, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (165.82, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (269.88, 124.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.276 CPU sec, 5.089 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 11574, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (171.13, 99.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (273.21, 125.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.398 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12263, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 159, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 303) [M::mem_pestat] mean and std.dev: (159.41, 51.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.76, 133.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.983 CPU sec, 5.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12707, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 153, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (184.06, 101.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 275, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (291.41, 133.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.783 CPU sec, 5.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12536, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 187, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (205.80, 128.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 272, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (288.38, 133.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.472 CPU sec, 6.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12629, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (188.93, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (283.99, 131.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.637 CPU sec, 7.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12569, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (219.92, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (286.39, 132.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.902 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12693, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (199.55, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (284.23, 132.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.341 CPU sec, 6.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12666, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 176, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (167.10, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (289.83, 132.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.766 CPU sec, 6.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12677, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 111, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (150.12, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 276, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (290.73, 133.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.779 CPU sec, 6.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12790, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 226, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (234.60, 121.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.65, 134.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.564 CPU sec, 6.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12588, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (217.26, 146.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 277, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (291.89, 133.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.043 CPU sec, 6.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12580, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (167.39, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (289.74, 132.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.266 CPU sec, 5.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12400, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 178, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (214.67, 128.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (277.30, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.879 CPU sec, 5.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12391, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (155.39, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (275.44, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.967 CPU sec, 5.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12061, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 204, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (197.23, 103.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (274.28, 126.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.206 CPU sec, 6.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11607, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 153, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 298) [M::mem_pestat] mean and std.dev: (148.78, 49.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (277.25, 127.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.744 CPU sec, 6.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12146, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 196, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (181.00, 99.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 268, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (285.84, 129.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.894 CPU sec, 6.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12570, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 175, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (206.90, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (288.00, 131.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.247 CPU sec, 7.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12620, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 196, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (189.64, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (285.05, 132.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.227 CPU sec, 5.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12662, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (226.28, 155.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 270, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (287.62, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.518 CPU sec, 6.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12726, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 170, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (209.16, 117.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 271, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (287.61, 132.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.214 CPU sec, 7.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12606, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 175, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (188.93, 109.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (288.36, 133.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.597 CPU sec, 6.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12541, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (193.04, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (289.22, 132.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.284 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12730, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 200, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (186.19, 126.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (289.82, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.439 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12805, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 208, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (196.81, 111.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (289.00, 131.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.137 CPU sec, 6.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12594, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 188, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (190.07, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (288.62, 132.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.065 CPU sec, 7.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12663, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 157, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (155.18, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (279.57, 128.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.986 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12470, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 184, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (178.72, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.84, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.041 CPU sec, 6.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 11968, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 138, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (155.93, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (267.64, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.656 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11726, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 178, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (176.88, 104.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 240, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.38, 121.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.887 CPU sec, 6.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 11403, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (134.44, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (262.97, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.336 CPU sec, 6.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12702, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (152.39, 81.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (284.63, 130.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.533 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12513, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 167, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (211.05, 145.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (282.22, 130.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.786 CPU sec, 7.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12424, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 144, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (176.75, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (280.74, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.745 CPU sec, 7.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12538, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 172, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (184.84, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (280.92, 129.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.665 CPU sec, 5.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12397, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 172, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (189.25, 109.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (279.03, 129.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.315 CPU sec, 5.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12486, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 165, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (186.39, 105.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.69, 131.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.519 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12649, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 185, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (201.00, 89.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 266, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (284.19, 129.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.479 CPU sec, 6.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12625, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 215, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (201.00, 96.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (285.40, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.177 CPU sec, 5.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12640, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (171.95, 120.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 273, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (287.38, 131.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.402 CPU sec, 7.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12554, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 168, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (184.82, 102.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (287.40, 131.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.325 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12325, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 154, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (182.06, 106.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.89, 124.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.620 CPU sec, 6.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12182, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 141, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (161.46, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (266.79, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.438 CPU sec, 7.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11989, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 165, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (154.30, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 238, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (261.93, 120.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.223 CPU sec, 5.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11417, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 141, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (151.50, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (261.08, 119.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.769 CPU sec, 7.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12031, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 147, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (181.27, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (277.38, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.747 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12702, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 174, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (186.28, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (286.89, 132.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.085 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12621, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 208, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (196.83, 90.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (281.78, 130.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.483 CPU sec, 5.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12586, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (180.61, 85.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (279.44, 129.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.643 CPU sec, 5.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12685, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 182, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (197.65, 117.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 261, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (280.32, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.845 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12651, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 187, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (200.95, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (280.68, 130.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.831 CPU sec, 5.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12383, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 169, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (185.41, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (284.51, 129.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.188 CPU sec, 7.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12594, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 176, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (198.08, 109.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 270, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (286.89, 130.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.106 CPU sec, 6.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12647, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 148, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (184.05, 146.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (285.51, 130.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.083 CPU sec, 6.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12821, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 197, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (164.88, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (284.88, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.865 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12387, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 257, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547) [M::mem_pestat] mean and std.dev: (255.73, 229.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (282.66, 131.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.457 CPU sec, 7.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12362, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 174, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (198.06, 128.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.76, 124.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.139 CPU sec, 5.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12208, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 139, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (173.40, 140.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (271.31, 124.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.491 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12137, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 126, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (147.74, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (269.65, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.753 CPU sec, 5.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11553, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 177, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (191.00, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.87, 122.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.360 CPU sec, 6.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12485, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 162, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (179.17, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (280.43, 129.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.427 CPU sec, 5.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12653, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 192, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (216.39, 125.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (285.67, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.514 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12691, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 106, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (127.86, 78.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.57, 129.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.833 CPU sec, 5.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12669, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 204, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (183.88, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (281.10, 128.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.145 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12487, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 130, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (145.00, 87.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (280.88, 128.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.772 CPU sec, 5.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12496, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 120, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (123.96, 52.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 265, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (284.16, 129.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.039 CPU sec, 5.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12601, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (138.21, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.06, 129.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.633 CPU sec, 5.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12746, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 174, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (195.04, 115.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (285.15, 130.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.827 CPU sec, 5.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12845, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 219, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (216.73, 123.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (283.84, 131.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.346 CPU sec, 5.942 real sec [M::process] read 8302 sequences (1253602 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12602, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 164, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (186.92, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (284.87, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.716 CPU sec, 5.099 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 1579, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 262, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (281.39, 130.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 8302 reads in 24.189 CPU sec, 1.449 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21175\tSM:UG10S2P415\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21175_HLKNYBGXX_UG10S2P415_R1.fastq.gz 5347_7332_21175_HLKNYBGXX_UG10S2P415_R2.fastq.gz [main] Real time: 2191.242 sec; CPU: 57928.783 sec Finished alignement of UG10S2P415 at Wed Apr 3 13:05:47 2019 Sorting BAM file started at Wed Apr 3 13:05:47 2019 samtools sort -@ 30 -m 4G UG10S2P415_unsorted.bam -o UG10S2P415.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 13:05:47 2019 rm UG10S2P415_unsorted.bam rm 5347_7332_21175_HLKNYBGXX_UG10S2P415_R1.fastq.gz rm 5347_7332_21175_HLKNYBGXX_UG10S2P415_R2.fastq.gz samtools index UG10S2P415.bam Finished BAM file generation of the sample - UG10S2P415 Processing fastq files for the sample - UG10S2P414 Reading 5347_7332_21176_HLKNYBGXX_UG10S2P414_R1.fastq.gz 5347_7332_21176_HLKNYBGXX_UG10S2P414_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21176\tSM:UG10S2P414\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21176_HLKNYBGXX_UG10S2P414_R1.fastq.gz 5347_7332_21176_HLKNYBGXX_UG10S2P414_R2.fastq.gz | samtools view -Sb1 - > UG10S2P414_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12406, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 139, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (167.68, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (214.98, 107.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.785 CPU sec, 7.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12515, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 98, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (108.40, 59.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (210.65, 102.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.366 CPU sec, 7.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12743, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (175.95, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (215.20, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.884 CPU sec, 6.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12847, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 121, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (112.10, 48.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (216.17, 107.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.348 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12696, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 118, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (121.91, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (216.94, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.669 CPU sec, 7.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12876, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 161, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (156.19, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.43, 105.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.625 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12522, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 93, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (113.17, 53.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.85, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.878 CPU sec, 7.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12735, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 127, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (152.07, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.69, 106.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.031 CPU sec, 7.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12702, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (120.95, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (214.87, 105.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.069 CPU sec, 6.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12745, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 127, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (157.19, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.08, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.675 CPU sec, 6.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12804, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 119, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (147.00, 82.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (213.53, 104.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.682 CPU sec, 6.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12660, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 160, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (156.33, 67.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (212.69, 104.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.423 CPU sec, 7.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12841, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 145, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (157.40, 102.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.63, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.435 CPU sec, 5.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12922, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 152, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (171.10, 100.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (210.79, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.177 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12787, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 114, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (138.63, 88.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (212.25, 104.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.395 CPU sec, 7.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12859, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 116, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (167.62, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (211.85, 103.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.502 CPU sec, 5.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12849, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 170, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (161.69, 81.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (211.41, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.075 CPU sec, 7.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12856, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 143, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (183.86, 126.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (212.28, 104.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.524 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12525, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (176.96, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.09, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.068 CPU sec, 7.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12507, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 154, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (155.24, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (211.80, 105.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.733 CPU sec, 7.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12495, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 162, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (171.52, 85.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.40, 103.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.357 CPU sec, 6.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12654, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (137.17, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (214.82, 106.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.253 CPU sec, 6.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12758, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 155, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (147.00, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (215.31, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.464 CPU sec, 5.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12834, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 98, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (126.82, 86.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.62, 109.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.511 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12726, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 104, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (140.96, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.97, 105.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.689 CPU sec, 6.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12685, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (167.72, 120.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (216.26, 107.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.300 CPU sec, 7.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12737, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 157, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (137.62, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.29, 106.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.526 CPU sec, 6.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12772, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (139.39, 76.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.08, 106.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.540 CPU sec, 6.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12645, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 185, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (161.05, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.28, 105.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.311 CPU sec, 6.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12627, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 170, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (147.50, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.28, 104.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.316 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12793, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 120, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.69, 69.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (214.97, 106.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.186 CPU sec, 5.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12867, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 120, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (152.87, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (211.94, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.176 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12806, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 140, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (174.20, 98.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (210.24, 101.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.768 CPU sec, 5.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12797, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 171, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (166.00, 68.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (210.81, 103.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.174 CPU sec, 5.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12809, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 129, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (149.81, 74.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.57, 103.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.132 CPU sec, 7.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12704, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 125, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (123.23, 49.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (211.75, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.535 CPU sec, 6.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12578, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 110, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (131.22, 81.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (214.12, 104.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.742 CPU sec, 6.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12610, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 104, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (105.07, 47.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (214.10, 104.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.257 CPU sec, 6.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12709, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 203, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (188.83, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (215.58, 107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.499 CPU sec, 6.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12598, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 139, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (155.06, 81.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.41, 105.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.365 CPU sec, 6.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12789, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 105, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (123.50, 79.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.88, 106.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.376 CPU sec, 6.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12836, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (194.00, 147.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (215.01, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.946 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12657, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 164, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (163.67, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (216.71, 107.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.606 CPU sec, 6.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12631, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 153, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (174.12, 106.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (212.84, 105.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.727 CPU sec, 6.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12703, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 135, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (155.00, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (213.00, 104.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.137 CPU sec, 6.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12779, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (148.04, 87.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (212.44, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.167 CPU sec, 6.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12836, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 124, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (143.94, 86.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.78, 106.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.766 CPU sec, 7.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12743, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 153, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (201.00, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (211.26, 103.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.035 CPU sec, 6.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12707, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 174, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (166.19, 91.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.42, 103.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.578 CPU sec, 5.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12851, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 129, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (113.06, 40.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.44, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.604 CPU sec, 6.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12806, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 159, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (180.00, 106.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.74, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.822 CPU sec, 6.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12758, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 177, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (200.44, 109.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (211.28, 102.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.201 CPU sec, 7.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12821, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 229, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (232.36, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (207.28, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.267 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12783, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 151, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (141.35, 74.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.26, 102.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.776 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12554, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 156, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (155.55, 68.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.46, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.911 CPU sec, 6.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12837, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 171, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (194.17, 130.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (212.65, 103.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.047 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12641, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 139, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (142.10, 89.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (210.20, 104.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.362 CPU sec, 7.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12681, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (151.69, 77.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (213.51, 105.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.043 CPU sec, 7.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12715, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 210, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (158.88, 94.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.25, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.230 CPU sec, 5.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12931, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 179, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (185.42, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.95, 106.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.753 CPU sec, 6.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12718, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (159.00, 104.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.73, 105.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.993 CPU sec, 8.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12635, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 108, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (132.83, 72.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.05, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.622 CPU sec, 6.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12464, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 128, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (154.58, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (212.56, 105.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.000 CPU sec, 7.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12721, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (146.29, 64.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (211.11, 103.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.749 CPU sec, 7.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12612, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (133.70, 68.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.87, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.534 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12627, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (135.76, 79.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (210.90, 104.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.423 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12860, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (156.78, 68.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (208.93, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.657 CPU sec, 6.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12777, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 150, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (154.75, 99.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.97, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.946 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12909, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 101, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (110.60, 65.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.75, 100.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.916 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12645, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 154, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (166.54, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (210.86, 102.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.065 CPU sec, 6.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12722, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 146, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (158.79, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (208.76, 101.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.610 CPU sec, 5.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12660, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 136, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (142.50, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.81, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.345 CPU sec, 11.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12449, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 154, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (171.22, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (207.71, 100.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.376 CPU sec, 7.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12779, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 122, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (144.56, 86.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (209.64, 102.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.262 CPU sec, 7.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12642, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 117, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (108.12, 42.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (208.21, 101.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.494 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12820, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (126.90, 68.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.78, 101.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.289 CPU sec, 6.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12873, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (132.11, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.24, 103.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.321 CPU sec, 7.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12677, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 143, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (179.00, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.21, 104.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.627 CPU sec, 6.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12708, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 153, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (140.92, 57.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.30, 105.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.337 CPU sec, 6.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12612, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 141, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (154.80, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.25, 104.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.754 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12673, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 123, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (127.72, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (211.39, 103.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.502 CPU sec, 7.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12675, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 131, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (185.46, 156.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (211.82, 104.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.871 CPU sec, 7.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12791, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 127, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (153.92, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (211.22, 101.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.735 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12702, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 104, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (136.92, 86.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (211.81, 103.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.232 CPU sec, 8.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12870, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 85, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (88.00, 59.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.20, 103.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.757 CPU sec, 7.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12924, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 73, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (124.80, 103.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.33, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.825 CPU sec, 6.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12775, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 109, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (117.59, 79.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (210.05, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.004 CPU sec, 5.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13057, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 169, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (153.11, 81.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (211.97, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.418 CPU sec, 7.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12793, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 148, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (157.54, 114.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.19, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.399 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12576, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 150, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (150.12, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (207.82, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.632 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12403, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 93, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (84.53, 36.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (207.53, 100.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.636 CPU sec, 6.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12537, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (190.89, 133.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.48, 101.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.670 CPU sec, 6.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12569, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (111.85, 40.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (210.03, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.940 CPU sec, 6.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12658, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 138, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (209.18, 145.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.89, 103.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.157 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12735, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 138, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (136.44, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.95, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.946 CPU sec, 6.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12638, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (147.18, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (210.78, 104.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.536 CPU sec, 7.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12559, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 134, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (186.76, 127.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.79, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.921 CPU sec, 6.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12382, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 153, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (166.81, 89.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (211.58, 103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.162 CPU sec, 7.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12434, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 80, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (121.88, 75.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (212.14, 105.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.502 CPU sec, 6.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12480, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 111, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (122.65, 58.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.63, 100.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.689 CPU sec, 6.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12702, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 126, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (161.65, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.27, 102.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.237 CPU sec, 5.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12646, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 145, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (158.48, 63.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.98, 101.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.797 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12743, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 107, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (127.36, 74.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (208.05, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.449 CPU sec, 7.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12819, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 98, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (100.93, 46.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (208.45, 101.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.625 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12736, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 91, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (92.39, 44.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.78, 100.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.581 CPU sec, 5.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12814, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (136.11, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (206.80, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.847 CPU sec, 6.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12749, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 157, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (161.20, 101.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.62, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.156 CPU sec, 4.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12785, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.97, 105.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.051 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12662, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 150, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (169.67, 111.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 189, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (217.30, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.770 CPU sec, 6.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12586, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 137, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (144.72, 84.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.05, 108.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.859 CPU sec, 5.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12777, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 170, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (187.50, 169.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 188, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (215.45, 105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.302 CPU sec, 6.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12745, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 112, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (159.84, 112.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (215.02, 105.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.559 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12686, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 129, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (148.07, 92.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (214.83, 105.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.552 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12711, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 91, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (131.79, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.63, 107.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.655 CPU sec, 6.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12624, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (126.82, 87.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (215.78, 107.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.425 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12674, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 95, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (93.88, 40.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.70, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.393 CPU sec, 7.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12760, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 119, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (139.20, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (213.05, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.023 CPU sec, 7.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12684, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 110, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (109.50, 60.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (213.51, 104.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.715 CPU sec, 5.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12800, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (165.20, 77.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (213.28, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.450 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12779, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 126, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (131.54, 58.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.54, 106.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.247 CPU sec, 6.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12792, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (138.23, 90.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (210.76, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.668 CPU sec, 6.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12712, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (150.04, 92.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (207.71, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.232 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12731, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 139, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (138.53, 74.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (208.46, 100.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.244 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12583, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (103.21, 47.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (205.36, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.545 CPU sec, 6.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12274, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 169, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (161.29, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (203.98, 96.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.758 CPU sec, 6.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12421, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (152.11, 74.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (213.32, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.856 CPU sec, 6.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12533, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 216, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (194.47, 109.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (216.56, 108.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.152 CPU sec, 7.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12623, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 121, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (117.56, 76.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (216.87, 106.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.357 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12627, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 134, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (160.95, 104.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (217.32, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.334 CPU sec, 6.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12585, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 126, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (146.90, 64.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (217.38, 108.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.852 CPU sec, 7.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12658, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 125, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (137.33, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 190, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (217.76, 108.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.717 CPU sec, 6.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12669, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 100, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (112.71, 73.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 190, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (218.42, 109.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.562 CPU sec, 6.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12739, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (158.94, 112.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (216.98, 108.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.484 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12697, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 135, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (148.55, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (215.97, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.769 CPU sec, 6.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12673, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 159, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (173.33, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (215.72, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.450 CPU sec, 7.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12841, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (171.47, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (216.33, 106.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.372 CPU sec, 6.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12764, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 138, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (133.64, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (214.57, 107.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.808 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12686, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 177, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (153.50, 101.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (212.23, 105.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.074 CPU sec, 6.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12951, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 149, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (181.95, 119.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (208.98, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.997 CPU sec, 6.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12733, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (143.72, 112.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (206.51, 100.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.695 CPU sec, 5.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12508, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (115.32, 60.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (206.87, 99.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.133 CPU sec, 6.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12498, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (130.46, 62.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (204.78, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.571 CPU sec, 6.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12105, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 144, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (145.31, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (204.16, 96.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.997 CPU sec, 6.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12396, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 181, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (178.15, 88.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (216.25, 106.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.938 CPU sec, 7.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12561, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (108.95, 55.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (219.20, 108.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.080 CPU sec, 7.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12799, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (132.53, 58.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 191, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (217.80, 108.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.562 CPU sec, 6.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12587, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 190, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (186.68, 99.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (218.82, 108.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.398 CPU sec, 6.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12663, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 158, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (175.06, 121.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (217.53, 109.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.659 CPU sec, 6.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12668, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 145, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (128.38, 53.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (216.27, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.130 CPU sec, 6.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12555, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (146.44, 63.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (215.07, 106.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.256 CPU sec, 6.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12647, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 108, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (119.94, 54.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 188, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (216.75, 106.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.476 CPU sec, 6.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12698, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 102, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (136.95, 88.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.15, 105.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.445 CPU sec, 6.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12765, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 102, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (118.00, 59.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (215.56, 106.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.805 CPU sec, 6.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12608, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 128, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (128.25, 82.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.75, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.979 CPU sec, 6.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12634, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (157.50, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (213.84, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.056 CPU sec, 5.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12692, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 84, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (117.09, 74.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (209.85, 102.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.170 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12707, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 172, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (159.81, 76.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (209.29, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.757 CPU sec, 6.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12725, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 148, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (135.70, 90.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (208.69, 101.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.073 CPU sec, 5.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12702, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (150.40, 98.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.46, 99.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.660 CPU sec, 5.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12306, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 176, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (175.19, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (202.63, 96.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.013 CPU sec, 6.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12081, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 166, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (155.87, 68.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 179, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (205.13, 98.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.315 CPU sec, 6.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12701, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 154, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (152.75, 92.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (213.88, 105.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.752 CPU sec, 5.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12444, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (137.90, 84.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (215.19, 105.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.057 CPU sec, 7.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12594, 8, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 141, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (153.00, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (211.25, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.444 CPU sec, 5.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12474, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 193) [M::mem_pestat] mean and std.dev: (85.33, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.13, 105.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.163 CPU sec, 6.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12517, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (148.58, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.67, 105.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.876 CPU sec, 7.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12691, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 164, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (160.58, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (212.07, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.911 CPU sec, 6.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12654, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 139, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (165.53, 112.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.30, 105.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.973 CPU sec, 7.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12581, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 114, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (145.33, 100.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (214.95, 106.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.413 CPU sec, 5.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12786, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 132, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (142.77, 81.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (214.37, 105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.089 CPU sec, 6.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12654, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 93, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (150.12, 106.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (212.92, 105.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.973 CPU sec, 6.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12758, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 143, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (172.93, 149.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.75, 105.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.000 CPU sec, 7.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12761, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 162, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (139.45, 56.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (210.86, 101.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.644 CPU sec, 7.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12738, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 187, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (180.00, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (209.51, 102.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.064 CPU sec, 6.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12724, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 154, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (165.26, 118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (205.30, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.697 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12507, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (139.41, 81.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (204.74, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.947 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12539, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 199, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (210.32, 120.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (201.97, 95.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.869 CPU sec, 5.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12341, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 121, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (119.93, 54.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.32, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.780 CPU sec, 6.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12282, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 86, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (105.31, 54.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (204.41, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.827 CPU sec, 6.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12491, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 169, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (194.72, 107.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (214.58, 105.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.790 CPU sec, 6.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12552, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 164, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (159.40, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.96, 105.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.246 CPU sec, 6.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12642, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (177.83, 112.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.74, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.397 CPU sec, 6.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12598, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 181, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (183.32, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (215.32, 105.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.936 CPU sec, 6.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12669, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 123, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (132.32, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (214.37, 106.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.784 CPU sec, 6.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12680, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 111, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (119.38, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (216.42, 108.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.123 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12642, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 138, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (148.95, 101.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (214.40, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.227 CPU sec, 7.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12616, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 160, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (176.22, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.09, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.503 CPU sec, 6.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12526, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (150.46, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (215.35, 106.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.760 CPU sec, 6.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12654, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (157.08, 98.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (213.00, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.648 CPU sec, 6.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12689, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (154.18, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (213.54, 105.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.626 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12617, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 139, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (149.83, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.74, 104.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.845 CPU sec, 7.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12788, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (167.60, 77.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.55, 102.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.668 CPU sec, 7.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12779, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 181, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (150.74, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (206.23, 99.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.072 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12886, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (206.31, 99.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.335 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12640, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 116, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (136.53, 83.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.29, 97.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.421 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12203, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 150, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (158.47, 72.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (200.88, 94.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.873 CPU sec, 6.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12044, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 177, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (169.73, 92.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (209.45, 102.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.760 CPU sec, 7.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12590, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 152, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (165.80, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (217.57, 108.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.746 CPU sec, 6.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12490, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 124, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (137.20, 91.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.91, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.747 CPU sec, 6.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12591, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (158.58, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.83, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.973 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12635, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 143, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (184.36, 128.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.35, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.977 CPU sec, 6.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12714, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (129.53, 74.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (212.60, 104.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.108 CPU sec, 6.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12437, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (124.73, 57.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (215.63, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.239 CPU sec, 6.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12418, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 110, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (123.62, 65.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.82, 106.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.152 CPU sec, 7.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12504, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 92, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (96.69, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (214.45, 106.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.605 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12535, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 152, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (158.48, 98.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (212.66, 104.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.430 CPU sec, 7.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12647, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 203, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (185.71, 146.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.33, 105.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.549 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12670, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 168, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (178.67, 90.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.43, 103.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.990 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12651, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 159, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (162.24, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (209.38, 102.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.939 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12754, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 133, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (148.76, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (208.24, 100.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.733 CPU sec, 5.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12524, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 99, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (110.80, 70.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (208.38, 101.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.809 CPU sec, 5.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12594, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 104, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (149.54, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (203.76, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.469 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12387, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 123, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (140.00, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (201.72, 94.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.764 CPU sec, 7.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12370, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 136, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (130.04, 78.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (201.49, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.863 CPU sec, 6.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12532, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 104, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (119.33, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (207.53, 98.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.342 CPU sec, 6.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12901, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 162, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (169.48, 136.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (210.73, 102.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.209 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12838, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 145, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (162.26, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (209.70, 102.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.364 CPU sec, 5.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12718, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 166, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (177.84, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.11, 102.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.280 CPU sec, 7.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12835, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 134, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (135.50, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (207.50, 99.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.337 CPU sec, 6.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12830, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 118, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (131.00, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (209.85, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.542 CPU sec, 5.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12611, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 130, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (122.87, 60.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (214.46, 106.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.069 CPU sec, 6.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12570, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 130, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (143.26, 77.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (214.55, 106.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.870 CPU sec, 6.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12646, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 131, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (144.00, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (214.87, 106.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.318 CPU sec, 6.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12837, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 162, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (161.20, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.33, 103.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.479 CPU sec, 6.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12843, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 142, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (153.65, 68.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (211.97, 103.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.749 CPU sec, 6.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12824, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (168.18, 91.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.36, 102.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.018 CPU sec, 7.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12532, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 134, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (167.87, 156.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (208.16, 101.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.629 CPU sec, 6.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12583, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 143, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (141.43, 54.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.49, 104.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.949 CPU sec, 6.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12642, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 86, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (122.19, 78.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.84, 104.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.323 CPU sec, 8.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12766, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (139.81, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (212.64, 104.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.412 CPU sec, 6.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12781, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 195, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (175.47, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (214.97, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.339 CPU sec, 9.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12613, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (139.17, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (212.50, 103.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.371 CPU sec, 6.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12834, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (163.92, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (210.18, 103.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.988 CPU sec, 5.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12874, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 151, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (160.19, 78.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.43, 101.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.089 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12809, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 138, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (159.39, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (208.00, 100.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.356 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12780, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 100, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (131.61, 94.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (209.68, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.683 CPU sec, 6.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12689, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (127.35, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (207.04, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.986 CPU sec, 5.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12662, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 154, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (157.46, 60.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (215.02, 105.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.044 CPU sec, 7.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12824, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 106, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (150.54, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.61, 105.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.706 CPU sec, 7.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12610, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 222, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (190.41, 100.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (213.06, 104.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.661 CPU sec, 6.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12763, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 155, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (176.85, 91.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.57, 107.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.905 CPU sec, 7.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12719, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 144, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (161.17, 102.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.54, 102.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.799 CPU sec, 5.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12837, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 157, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (154.17, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.14, 105.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.630 CPU sec, 6.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12570, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 167, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (178.93, 100.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (214.64, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.782 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12513, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (135.83, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.14, 103.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.034 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12619, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 119, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (142.82, 79.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (210.31, 102.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.538 CPU sec, 6.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12679, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (124.10, 60.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.77, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.485 CPU sec, 6.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12691, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 130, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (170.33, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.68, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.930 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12615, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 128, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (153.57, 87.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.01, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.949 CPU sec, 6.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12894, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 103, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (118.24, 64.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.72, 105.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.345 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12896, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 149, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (174.17, 139.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (209.20, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.884 CPU sec, 5.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12615, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 112, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (153.21, 94.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.41, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.113 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12788, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 139, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (147.12, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.74, 100.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.482 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12828, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 150, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (151.71, 106.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (205.86, 100.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.685 CPU sec, 5.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12683, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (130.84, 62.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (211.07, 103.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.732 CPU sec, 6.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12805, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 154, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (226.53, 161.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (214.52, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.307 CPU sec, 6.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12749, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 177, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (202.31, 86.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.41, 105.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.777 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12625, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 139, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (186.35, 116.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.31, 105.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.025 CPU sec, 8.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12764, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (144.21, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (214.27, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.512 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12941, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 175, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (196.00, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.81, 104.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.572 CPU sec, 6.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12573, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (115.45, 56.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (211.26, 103.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.830 CPU sec, 8.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12532, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 125, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (136.47, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (213.23, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.083 CPU sec, 6.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12714, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 160, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (166.26, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (209.35, 102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.545 CPU sec, 6.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12684, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 159, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (165.31, 105.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (213.76, 105.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.001 CPU sec, 5.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12893, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 133, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (145.00, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.25, 105.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.991 CPU sec, 6.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12706, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 82, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (107.82, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.35, 104.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.935 CPU sec, 6.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12567, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 158, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (165.32, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.03, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.248 CPU sec, 6.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12668, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (169.74, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (208.80, 100.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.080 CPU sec, 6.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12838, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (163.76, 101.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (210.20, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.261 CPU sec, 6.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12746, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 110, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (116.83, 55.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (208.66, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.162 CPU sec, 5.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12740, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 101, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (142.65, 97.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (207.11, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.876 CPU sec, 5.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12478, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 164, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (161.63, 95.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (209.03, 102.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.978 CPU sec, 7.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12733, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (152.12, 63.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (215.62, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.667 CPU sec, 7.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12631, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 97, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (105.22, 67.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (210.76, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.436 CPU sec, 7.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12516, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (139.31, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.08, 103.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.617 CPU sec, 6.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12559, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 109, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (131.00, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.47, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.451 CPU sec, 6.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12617, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 125, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (128.67, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (211.79, 102.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.386 CPU sec, 6.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12648, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 103, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (122.59, 71.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (206.17, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.162 CPU sec, 6.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12391, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 166, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (158.00, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (203.71, 96.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.077 CPU sec, 6.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12470, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 139, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (160.56, 104.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (206.89, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.329 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12625, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 98, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (132.95, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.22, 99.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.259 CPU sec, 5.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12731, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 126, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (134.43, 81.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (209.82, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.602 CPU sec, 7.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12692, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (130.00, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (210.98, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.576 CPU sec, 7.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12836, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 121, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (138.90, 94.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.41, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.522 CPU sec, 7.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12792, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (138.19, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (208.52, 100.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.262 CPU sec, 6.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12770, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 167, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (180.32, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (209.62, 100.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.486 CPU sec, 7.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12740, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 145, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (154.42, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (205.90, 98.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.256 CPU sec, 6.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12932, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 153, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (144.36, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (205.70, 98.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.817 CPU sec, 5.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12726, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (161.70, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (208.50, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.602 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12606, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (155.61, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.53, 106.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.507 CPU sec, 6.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12585, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 113, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (147.47, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (210.14, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.630 CPU sec, 8.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12542, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 97, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (117.00, 71.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (213.34, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.854 CPU sec, 6.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12846, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (184.16, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (209.57, 103.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.983 CPU sec, 6.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12772, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 132, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (141.45, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (211.46, 104.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.969 CPU sec, 6.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12881, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 78, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (82.41, 54.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (209.01, 100.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.597 CPU sec, 6.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12394, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (120.30, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (207.28, 99.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.549 CPU sec, 5.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12632, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 113, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (105.10, 45.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (205.10, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.724 CPU sec, 6.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12542, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 157, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (160.40, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.53, 100.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.023 CPU sec, 7.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12830, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (139.85, 76.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (207.71, 101.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.323 CPU sec, 6.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12687, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 156, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (199.69, 144.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (210.19, 102.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.719 CPU sec, 5.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12674, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 196, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (206.75, 121.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (210.56, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.792 CPU sec, 7.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12789, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 127, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (115.53, 49.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (206.42, 99.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.408 CPU sec, 5.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12894, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (152.06, 108.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (206.58, 98.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.907 CPU sec, 7.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12793, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (128.05, 45.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (206.11, 99.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.401 CPU sec, 5.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12804, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 90, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (95.33, 55.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.70, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.031 CPU sec, 6.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12725, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (122.36, 76.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (205.87, 98.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.392 CPU sec, 6.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12645, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 138, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (152.31, 101.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (209.85, 103.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.151 CPU sec, 6.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12632, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 164, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (150.50, 90.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (208.95, 102.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.480 CPU sec, 6.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12480, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 150, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (171.11, 110.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (210.78, 103.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.032 CPU sec, 7.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12544, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 140, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (131.75, 73.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.76, 102.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.969 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12667, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (157.29, 108.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (212.09, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.638 CPU sec, 6.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12622, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (186.53, 126.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.85, 101.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.736 CPU sec, 5.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12583, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 115, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (132.82, 90.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (207.68, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.874 CPU sec, 6.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12380, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 231, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (195.29, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.78, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.463 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12643, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (136.67, 64.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (205.88, 99.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.047 CPU sec, 6.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12706, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 132, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (132.12, 60.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (209.10, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.885 CPU sec, 6.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12838, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 120, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (135.53, 65.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (210.47, 103.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.093 CPU sec, 6.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12674, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (165.47, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.41, 103.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.901 CPU sec, 6.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12891, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (154.83, 86.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (206.69, 100.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.381 CPU sec, 6.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12672, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 151, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (168.35, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (204.68, 98.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.730 CPU sec, 6.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12749, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 127, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (150.71, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (206.99, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.800 CPU sec, 7.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12694, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 123, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (99.86, 45.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (203.11, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.485 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12460, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (203.69, 95.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.067 CPU sec, 7.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12297, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 147, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (171.48, 102.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (207.03, 99.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.441 CPU sec, 7.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12780, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 100, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (102.11, 55.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.62, 106.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.912 CPU sec, 6.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12783, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 108, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (145.60, 89.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.16, 107.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.273 CPU sec, 6.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12722, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 152, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (136.67, 56.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.77, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.465 CPU sec, 7.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12828, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 107, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (139.88, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (213.63, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.733 CPU sec, 6.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12712, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 160, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (149.70, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (209.95, 102.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.836 CPU sec, 5.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12814, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 172, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (150.60, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (210.61, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.267 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12528, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 105, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (135.31, 88.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (212.65, 104.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.478 CPU sec, 6.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12827, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (137.79, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.44, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.503 CPU sec, 7.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12604, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (147.50, 67.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (214.35, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.353 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12664, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (142.04, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (215.48, 106.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.061 CPU sec, 6.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12523, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 153, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (167.37, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.33, 105.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.678 CPU sec, 6.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12700, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 99, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 195) [M::mem_pestat] mean and std.dev: (85.50, 35.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.98, 103.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.014 CPU sec, 6.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12637, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 135, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (125.80, 36.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.34, 102.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.864 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12698, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (96.48, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.42, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.431 CPU sec, 5.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12649, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 105, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (115.12, 65.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (206.27, 98.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.709 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12127, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 151, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (141.00, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (203.73, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.009 CPU sec, 6.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12276, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 123, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (145.50, 70.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (204.80, 96.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.475 CPU sec, 6.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12610, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 180, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (169.68, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.10, 106.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.251 CPU sec, 8.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12607, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 140, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (161.13, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (215.75, 105.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.187 CPU sec, 6.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12739, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 151, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (161.96, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (214.86, 106.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.966 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12706, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 163, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (141.42, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (213.38, 104.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.637 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12761, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 115, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (135.08, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.55, 104.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.229 CPU sec, 6.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12897, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (180.60, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.47, 102.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.375 CPU sec, 5.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12670, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (130.00, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (213.66, 106.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.946 CPU sec, 7.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12517, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (144.61, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (215.90, 107.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.192 CPU sec, 7.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12592, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 68, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (133.25, 128.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (214.61, 106.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.229 CPU sec, 8.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12643, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 141, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (157.19, 110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (216.73, 108.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.579 CPU sec, 6.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12675, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (152.94, 86.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.19, 107.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.760 CPU sec, 6.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12620, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 157, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (148.91, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (216.43, 107.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.331 CPU sec, 6.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12792, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 102, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (119.96, 76.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.77, 102.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.488 CPU sec, 5.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12735, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 170, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (166.12, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.81, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.678 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12706, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 82, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (106.24, 63.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (206.41, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.933 CPU sec, 5.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12554, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 154, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (141.65, 57.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (204.43, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.525 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12456, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 148, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (139.62, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (206.29, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.141 CPU sec, 5.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12451, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (160.77, 58.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (210.74, 102.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.143 CPU sec, 6.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12852, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 176, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (172.40, 72.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.54, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.197 CPU sec, 6.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12722, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (136.88, 68.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (213.25, 105.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.190 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12740, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 125, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (163.69, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (213.70, 104.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.526 CPU sec, 7.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12897, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 233, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (208.82, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.54, 103.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.758 CPU sec, 6.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12756, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (140.36, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (209.32, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.277 CPU sec, 5.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12710, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (166.45, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (212.88, 105.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.500 CPU sec, 6.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12685, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 141, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (139.42, 43.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (216.92, 107.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.784 CPU sec, 7.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12763, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 141, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (129.39, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (215.39, 105.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.360 CPU sec, 6.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12782, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 116, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (127.86, 68.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (215.37, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.895 CPU sec, 6.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12700, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 106, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (157.50, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (213.23, 104.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.546 CPU sec, 6.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12831, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 139, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (157.11, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.67, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.954 CPU sec, 5.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12654, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 131, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (153.35, 106.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (207.51, 100.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.803 CPU sec, 6.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12763, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 94, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (118.07, 93.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (205.83, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.263 CPU sec, 5.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12606, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (164.05, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (203.84, 96.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.040 CPU sec, 5.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12512, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (162.11, 83.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (201.38, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.003 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12244, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 128, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (169.43, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (199.06, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.743 CPU sec, 5.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12329, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (161.35, 85.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.25, 95.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.508 CPU sec, 6.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12616, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 126, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (138.21, 86.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (212.22, 104.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.581 CPU sec, 6.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12660, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 92, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (99.88, 50.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.41, 105.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.184 CPU sec, 6.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12596, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 92, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (112.29, 70.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (211.28, 104.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.068 CPU sec, 8.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12609, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 185, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (167.40, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (210.16, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.012 CPU sec, 6.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12632, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 131, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (136.79, 69.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.74, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.645 CPU sec, 6.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12638, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 107, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (127.53, 56.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.36, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.434 CPU sec, 6.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12617, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 154, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (181.47, 93.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (212.74, 104.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.288 CPU sec, 5.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12500, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 91, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (102.29, 61.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (209.54, 102.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.677 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12456, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 102, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (117.11, 65.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (213.85, 105.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.593 CPU sec, 6.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12642, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 171, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (190.93, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (215.11, 106.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.250 CPU sec, 6.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12639, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (147.36, 67.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.76, 105.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.820 CPU sec, 7.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12775, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 139, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (150.19, 75.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (209.00, 102.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.078 CPU sec, 6.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12740, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (152.64, 121.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (205.23, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.699 CPU sec, 5.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12771, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 138, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (153.71, 105.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (203.26, 96.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.796 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12398, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (140.10, 86.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 178, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (202.08, 96.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.272 CPU sec, 5.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12242, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (145.43, 77.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 175, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (197.35, 91.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.868 CPU sec, 7.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12080, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 182, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (180.78, 92.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (201.10, 94.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.578 CPU sec, 6.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12663, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 127, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (124.28, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (213.67, 105.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.465 CPU sec, 7.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12659, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 130, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (133.08, 54.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.01, 104.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.784 CPU sec, 6.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12638, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 161, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (167.45, 85.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (212.00, 104.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.744 CPU sec, 6.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12746, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 166, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (156.06, 81.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.61, 105.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.069 CPU sec, 6.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12635, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 169, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (170.18, 96.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (209.59, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.840 CPU sec, 6.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12764, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 151, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (177.47, 103.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (209.12, 101.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.632 CPU sec, 6.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12518, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 94, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (125.88, 69.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (213.47, 104.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.544 CPU sec, 7.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12641, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (155.88, 86.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (212.80, 105.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.821 CPU sec, 6.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12638, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 117, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (126.72, 64.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (213.88, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.092 CPU sec, 7.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12671, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 138, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (154.64, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (212.22, 103.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.934 CPU sec, 6.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12568, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (117.10, 60.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (212.84, 103.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.380 CPU sec, 6.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12562, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (141.20, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (210.71, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.470 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12852, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (129.94, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (208.91, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.720 CPU sec, 5.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12744, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 96, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (127.72, 74.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (205.90, 98.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.185 CPU sec, 6.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12697, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 151, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (162.27, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (203.61, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.290 CPU sec, 7.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12585, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (137.43, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.26, 94.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.323 CPU sec, 6.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12336, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 135, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (161.76, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (201.91, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.879 CPU sec, 6.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12680, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 164, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (194.92, 132.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.76, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.985 CPU sec, 6.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12807, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (149.53, 94.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.75, 103.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.155 CPU sec, 7.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12755, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 91, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (94.82, 53.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (211.00, 103.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.765 CPU sec, 7.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12747, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 138, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (174.65, 154.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (211.38, 102.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.442 CPU sec, 5.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12655, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 89, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (107.67, 62.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (211.19, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.683 CPU sec, 6.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12567, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 91, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (105.09, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (211.18, 104.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.348 CPU sec, 5.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12688, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (108.76, 58.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 184, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (210.45, 102.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.861 CPU sec, 7.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12663, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 76, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (105.82, 81.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (213.70, 104.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.780 CPU sec, 6.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12628, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 131, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (155.20, 79.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (212.59, 104.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.994 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12568, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 131, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (140.09, 88.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (211.79, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.333 CPU sec, 6.423 real sec [M::process] read 54576 sequences (8240976 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12665, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 178, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (166.56, 77.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (213.46, 105.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.651 CPU sec, 6.245 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 10468, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (164.13, 100.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (212.92, 105.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 54576 reads in 142.984 CPU sec, 5.635 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21176\tSM:UG10S2P414\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21176_HLKNYBGXX_UG10S2P414_R1.fastq.gz 5347_7332_21176_HLKNYBGXX_UG10S2P414_R2.fastq.gz [main] Real time: 2743.635 sec; CPU: 70763.293 sec Finished alignement of UG10S2P414 at Wed Apr 3 13:44:35 2019 Sorting BAM file started at Wed Apr 3 13:44:35 2019 samtools sort -@ 30 -m 4G UG10S2P414_unsorted.bam -o UG10S2P414.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 13:44:35 2019 rm UG10S2P414_unsorted.bam rm 5347_7332_21176_HLKNYBGXX_UG10S2P414_R1.fastq.gz rm 5347_7332_21176_HLKNYBGXX_UG10S2P414_R2.fastq.gz samtools index UG10S2P414.bam Finished BAM file generation of the sample - UG10S2P414 Processing fastq files for the sample - UG10S1P041 Reading 5347_7332_21177_HLKNYBGXX_UG10S1P041_R1.fastq.gz 5347_7332_21177_HLKNYBGXX_UG10S1P041_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21177\tSM:UG10S1P041\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21177_HLKNYBGXX_UG10S1P041_R1.fastq.gz 5347_7332_21177_HLKNYBGXX_UG10S1P041_R2.fastq.gz | samtools view -Sb1 - > UG10S1P041_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12031, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.58, 58.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 267.517 CPU sec, 11.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11808, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (144.80, 59.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 262.346 CPU sec, 9.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11697, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 85, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (124.69, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.74, 60.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 294.876 CPU sec, 10.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11002, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 128, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (120.60, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (144.94, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 314.326 CPU sec, 15.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11040, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 99, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (120.45, 48.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.55, 60.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.055 CPU sec, 9.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11109, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.30, 60.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 267.641 CPU sec, 9.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11387, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 110, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (110.91, 69.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (143.84, 59.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.513 CPU sec, 8.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12567, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 116, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (93.92, 49.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.00, 58.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.464 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12851, 9, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.62, 59.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.395 CPU sec, 7.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12379, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 110, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (140.07, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.33, 57.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.485 CPU sec, 8.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11895, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 95, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (120.14, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.55, 58.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 276.917 CPU sec, 9.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11781, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 82, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (91.08, 57.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.33, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 271.724 CPU sec, 9.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11533, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (177.43, 128.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.62, 58.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.634 CPU sec, 8.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11157, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 100, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (115.00, 54.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.58, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.791 CPU sec, 8.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11087, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 139, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (153.21, 87.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.01, 61.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 262.928 CPU sec, 9.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11466, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 95, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (108.30, 61.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.18, 58.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.283 CPU sec, 7.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12359, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 137, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (133.18, 63.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.19, 61.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.605 CPU sec, 5.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12672, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 113, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (114.87, 59.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.28, 59.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.328 CPU sec, 5.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12905, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 79, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (96.93, 53.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.14, 58.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.671 CPU sec, 5.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12100, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 83, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (125.31, 114.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.63, 57.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.031 CPU sec, 7.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11985, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (145.80, 61.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 187.282 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11755, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 171, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (183.30, 111.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.39, 61.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.518 CPU sec, 7.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11215, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (147.81, 63.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.379 CPU sec, 8.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11124, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 153, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (147.58, 72.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.23, 60.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 289.550 CPU sec, 9.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11348, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 148, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (176.42, 133.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (145.50, 60.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 285.085 CPU sec, 10.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11961, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 142, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (150.08, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.09, 59.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.595 CPU sec, 8.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12664, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 103, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (101.15, 61.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (141.32, 56.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.523 CPU sec, 6.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12838, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 123, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 209) [M::mem_pestat] mean and std.dev: (111.67, 38.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.39, 60.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.909 CPU sec, 7.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12437, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 110, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (119.35, 51.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.57, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.746 CPU sec, 6.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11914, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.85, 58.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 221.757 CPU sec, 8.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11687, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.21, 59.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 220.951 CPU sec, 7.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11306, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 143, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (117.77, 57.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.46, 59.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.817 CPU sec, 8.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10817, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (146.07, 60.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 273.843 CPU sec, 10.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 10732, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (144.34, 58.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 275.476 CPU sec, 9.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10854, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 85, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (85.82, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.33, 59.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 278.648 CPU sec, 9.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11349, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 93, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (101.50, 33.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.16, 62.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.121 CPU sec, 7.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12297, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 76, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (73.67, 31.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (145.05, 59.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.948 CPU sec, 7.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12606, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (142.60, 57.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.166 CPU sec, 5.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12381, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (145.86, 90.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.39, 59.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.440 CPU sec, 6.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11966, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.38, 59.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 231.581 CPU sec, 7.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11606, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 61, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (79.55, 49.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.94, 58.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.111 CPU sec, 7.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11362, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 74, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (91.13, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.40, 59.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.077 CPU sec, 8.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10924, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 83, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 141) [M::mem_pestat] mean and std.dev: (69.25, 16.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.40, 57.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.569 CPU sec, 9.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10783, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 84, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (121.40, 65.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.90, 58.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.584 CPU sec, 9.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11399, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 99, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (97.15, 34.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.79, 60.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.971 CPU sec, 8.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12313, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 76, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (90.00, 42.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.53, 59.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.692 CPU sec, 7.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12463, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 101, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (109.50, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (145.09, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.444 CPU sec, 6.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12660, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 110, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (169.73, 147.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (142.64, 57.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.424 CPU sec, 6.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11738, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (123.50, 74.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.80, 57.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.427 CPU sec, 7.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11580, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 99, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (105.80, 48.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.58, 57.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.484 CPU sec, 7.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11202, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 161, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (159.13, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (143.77, 58.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.694 CPU sec, 8.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10705, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 110, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (112.93, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.28, 57.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 269.151 CPU sec, 9.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10662, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 152, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (181.78, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (145.43, 59.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 292.176 CPU sec, 10.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 10830, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.43, 59.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 322.774 CPU sec, 12.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11048, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 78, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (88.70, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.31, 58.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 330.075 CPU sec, 11.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12303, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (124.33, 55.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (143.08, 57.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.836 CPU sec, 8.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12442, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 101, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (95.54, 46.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.94, 59.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.884 CPU sec, 8.139 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12090, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.42, 58.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 235.034 CPU sec, 8.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11307, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 138, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (146.76, 61.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 270.221 CPU sec, 9.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11268, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 105, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (119.40, 61.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.20, 60.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 272.224 CPU sec, 10.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11204, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 93, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (93.56, 35.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 138, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.82, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 304.899 CPU sec, 10.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10913, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 65, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (65.67, 19.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.21, 59.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 335.810 CPU sec, 11.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11111, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 97, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (94.50, 36.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.90, 58.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 307.767 CPU sec, 10.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11654, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.39, 58.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 282.761 CPU sec, 9.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12265, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.88, 60.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 255.991 CPU sec, 9.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12677, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 108, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 192) [M::mem_pestat] mean and std.dev: (100.92, 27.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.40, 56.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.549 CPU sec, 7.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12677, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 97, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (115.08, 79.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (143.05, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.148 CPU sec, 8.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11764, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 121, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (118.69, 77.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.13, 57.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.787 CPU sec, 7.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11191, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 92, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (102.64, 63.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 138, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.75, 62.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 277.717 CPU sec, 9.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11367, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 146, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (165.67, 131.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.21, 60.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 302.753 CPU sec, 10.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11077, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 138, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (147.52, 60.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 310.630 CPU sec, 11.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10973, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.16, 61.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 335.804 CPU sec, 11.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11313, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 87, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (137.00, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.82, 60.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 307.032 CPU sec, 10.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11752, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 85, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (95.89, 37.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (145.31, 59.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 278.394 CPU sec, 10.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12448, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (138.47, 84.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (144.70, 58.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.824 CPU sec, 8.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12753, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 73, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (95.09, 56.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.66, 56.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.170 CPU sec, 7.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12523, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 97, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (88.82, 38.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.74, 58.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.997 CPU sec, 7.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11288, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 59, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (68.44, 42.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (148.44, 62.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 284.226 CPU sec, 10.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11053, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 90, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (102.36, 64.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.98, 61.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 305.928 CPU sec, 10.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10912, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.27, 61.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 326.783 CPU sec, 12.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10990, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 162, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (138.56, 61.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (144.79, 58.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 349.111 CPU sec, 14.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10856, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.07, 61.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 324.194 CPU sec, 11.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11318, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 68, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (68.58, 23.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (145.09, 59.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 294.206 CPU sec, 10.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11865, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (139.00, 79.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.71, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 265.084 CPU sec, 9.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12856, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 128, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (125.80, 63.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.23, 58.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.701 CPU sec, 8.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12554, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (137.09, 100.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (142.31, 56.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.665 CPU sec, 8.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11941, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 154, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 278) [M::mem_pestat] mean and std.dev: (144.42, 52.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.83, 58.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.880 CPU sec, 8.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10987, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 102, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (101.43, 61.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.20, 57.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 294.664 CPU sec, 10.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11155, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 107, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (116.31, 91.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.03, 59.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 300.904 CPU sec, 10.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10831, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.64, 60.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 351.812 CPU sec, 11.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10601, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 84, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (96.30, 52.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.09, 59.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 396.420 CPU sec, 13.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10866, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 78, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (94.18, 54.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (143.91, 58.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 349.694 CPU sec, 11.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11184, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 145, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (152.55, 61.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.21, 59.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 307.971 CPU sec, 10.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11609, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 64, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (90.09, 60.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.69, 59.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.483 CPU sec, 9.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12626, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 134, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (154.78, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.10, 57.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.357 CPU sec, 7.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12773, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 89, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (103.53, 72.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.01, 56.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.058 CPU sec, 7.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11769, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 76, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (78.40, 30.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.40, 58.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.828 CPU sec, 7.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10936, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (136.64, 94.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (146.21, 62.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 318.543 CPU sec, 10.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10785, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 124, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (131.82, 72.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 138, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (146.34, 61.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 335.252 CPU sec, 11.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10987, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.58, 58.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 329.623 CPU sec, 12.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10714, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.86, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 370.840 CPU sec, 12.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10950, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 139, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (140.18, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.64, 60.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 335.794 CPU sec, 11.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11356, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 88, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (99.00, 48.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.03, 61.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 294.200 CPU sec, 10.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12014, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.91, 58.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 253.037 CPU sec, 8.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12670, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 139, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (142.68, 102.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.33, 58.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.465 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12565, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.34, 56.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 187.660 CPU sec, 6.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11335, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 135, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (129.74, 61.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.59, 58.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 265.881 CPU sec, 9.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10575, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 103, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (94.50, 34.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.78, 59.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 305.129 CPU sec, 10.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 10607, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 138, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (147.34, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 361.386 CPU sec, 12.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10571, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 162, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (152.93, 118.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (144.91, 58.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 373.064 CPU sec, 12.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10427, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (104.67, 49.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.24, 60.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 377.247 CPU sec, 13.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10605, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 115, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (128.91, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.06, 58.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 352.627 CPU sec, 12.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11047, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (112.27, 69.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.63, 59.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 318.821 CPU sec, 11.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11839, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 101, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (104.45, 33.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.03, 58.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.167 CPU sec, 9.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12546, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (122.64, 87.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (142.26, 56.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.407 CPU sec, 8.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12698, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (142.43, 56.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.542 CPU sec, 7.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12500, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (133.62, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.10, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.327 CPU sec, 7.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12744, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 130, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (130.29, 58.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.85, 59.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.369 CPU sec, 7.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12606, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (143.15, 57.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 209.998 CPU sec, 7.626 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11069, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (102.30, 54.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.49, 61.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 325.561 CPU sec, 12.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11186, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 124, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (139.92, 77.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.32, 60.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 310.995 CPU sec, 10.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11563, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.11, 60.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 284.935 CPU sec, 10.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11994, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.35, 58.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 262.582 CPU sec, 9.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11678, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 102, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (94.90, 35.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.35, 60.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 285.008 CPU sec, 9.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11453, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 157, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (162.09, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.89, 58.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 324.230 CPU sec, 11.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11926, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 107, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (152.23, 105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (144.91, 60.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 280.909 CPU sec, 9.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12777, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 101, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (82.69, 42.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.86, 60.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.375 CPU sec, 8.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12736, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 89, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (83.42, 34.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.36, 58.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.237 CPU sec, 6.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11664, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.70, 60.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 298.840 CPU sec, 11.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11327, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 167, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (172.50, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.49, 60.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 355.644 CPU sec, 13.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11619, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (115.45, 70.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.91, 59.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.351 CPU sec, 14.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12167, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 113, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (137.73, 92.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (143.90, 59.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 259.879 CPU sec, 9.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11971, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.18, 58.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 274.819 CPU sec, 9.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11655, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (131.00, 62.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (144.69, 60.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 290.942 CPU sec, 10.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11905, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.12, 58.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 275.705 CPU sec, 9.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12780, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 104, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (87.83, 35.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.86, 58.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.103 CPU sec, 7.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13052, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 127, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (107.36, 68.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.41, 57.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.691 CPU sec, 6.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11994, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 101, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (108.00, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.21, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.687 CPU sec, 8.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11412, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 166, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (152.73, 56.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.51, 60.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 307.233 CPU sec, 11.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11708, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 112, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (141.24, 98.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.89, 60.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.557 CPU sec, 9.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12122, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 104, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (90.50, 33.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (142.63, 57.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.864 CPU sec, 9.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11970, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.38, 58.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 266.949 CPU sec, 9.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11810, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 162, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (139.22, 58.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (144.94, 60.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 295.341 CPU sec, 10.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11808, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (148.50, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (145.78, 60.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 297.255 CPU sec, 10.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12233, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 111, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (136.92, 109.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (142.97, 58.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.359 CPU sec, 8.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12697, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 138, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (138.14, 83.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.15, 58.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.201 CPU sec, 7.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11968, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (130.10, 60.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.70, 58.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 289.362 CPU sec, 10.281 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 10797, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (122.00, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.23, 58.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 382.963 CPU sec, 18.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 10971, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 94, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (160.17, 133.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.14, 57.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 337.967 CPU sec, 11.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11011, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 88, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (87.50, 51.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.60, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 303.185 CPU sec, 10.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11747, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 109, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (111.62, 47.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (140.52, 54.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.923 CPU sec, 8.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11795, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (143.45, 58.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 285.623 CPU sec, 9.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11414, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 79, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (99.09, 54.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.90, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 347.215 CPU sec, 12.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12032, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.70, 59.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 260.276 CPU sec, 9.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12597, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 88, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (111.42, 81.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.32, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.717 CPU sec, 7.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12765, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 148, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (175.36, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.34, 58.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.291 CPU sec, 8.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11096, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (119.00, 85.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (144.55, 58.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 358.884 CPU sec, 12.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11016, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (166.46, 94.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.93, 58.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 388.085 CPU sec, 15.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11448, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 77, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (104.62, 53.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.07, 57.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 282.939 CPU sec, 10.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11894, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 98, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (148.55, 146.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.53, 58.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.619 CPU sec, 8.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11712, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (143.67, 76.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (142.45, 56.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.254 CPU sec, 9.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11666, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 107, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (117.29, 57.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.78, 58.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 309.289 CPU sec, 10.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11605, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (149.21, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (145.28, 59.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 297.038 CPU sec, 10.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12430, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 112, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (110.45, 46.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (142.34, 57.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.766 CPU sec, 8.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12703, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 217, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (189.67, 176.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.88, 58.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.027 CPU sec, 8.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11819, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 171, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (155.50, 94.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.32, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 268.262 CPU sec, 9.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11092, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.15, 60.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 329.956 CPU sec, 11.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11082, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.37, 59.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 310.287 CPU sec, 10.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11538, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (128.45, 48.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.58, 59.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 277.345 CPU sec, 9.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12027, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (141.48, 55.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 237.951 CPU sec, 8.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11808, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 94, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (128.50, 74.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.60, 60.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 274.933 CPU sec, 9.626 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11699, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (145.14, 60.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 299.829 CPU sec, 10.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12301, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (104.09, 54.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.14, 59.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.173 CPU sec, 7.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12642, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (128.67, 80.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.71, 57.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.518 CPU sec, 6.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12403, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (144.04, 57.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 220.311 CPU sec, 8.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10940, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (138.31, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.89, 58.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 305.621 CPU sec, 10.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11591, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.10, 58.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 295.114 CPU sec, 10.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11934, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 96, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (146.86, 133.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.22, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.139 CPU sec, 10.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11731, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 77, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (93.10, 55.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.06, 59.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.880 CPU sec, 9.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11292, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 167, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (177.40, 106.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.55, 60.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.251 CPU sec, 9.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11218, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (146.90, 62.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 292.013 CPU sec, 9.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12023, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (143.43, 58.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 264.104 CPU sec, 9.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12632, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 68, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152) [M::mem_pestat] mean and std.dev: (75.20, 29.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (143.07, 57.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.874 CPU sec, 7.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12655, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (143.81, 57.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 227.240 CPU sec, 8.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11256, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 178, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (152.00, 74.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.86, 60.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 335.046 CPU sec, 12.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11299, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 100, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (97.67, 57.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.62, 60.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 320.897 CPU sec, 11.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11457, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (140.31, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (144.85, 60.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 284.489 CPU sec, 9.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11694, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.77, 59.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 270.668 CPU sec, 9.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11021, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 78, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (76.00, 31.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (145.85, 61.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 285.573 CPU sec, 10.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11010, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (144.49, 59.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 323.112 CPU sec, 12.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11195, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 89, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (93.50, 52.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (147.40, 62.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 342.698 CPU sec, 11.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12374, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 92, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (101.71, 39.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.58, 57.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.563 CPU sec, 8.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12588, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (130.05, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (141.00, 55.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.176 CPU sec, 6.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12180, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 84, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (87.30, 34.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.82, 58.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.162 CPU sec, 8.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11302, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 80, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (80.62, 37.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.10, 60.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 305.089 CPU sec, 10.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11571, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 87, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (102.27, 53.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (144.83, 59.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 282.836 CPU sec, 9.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12007, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 100, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (124.25, 69.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.31, 61.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.778 CPU sec, 8.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11503, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 97, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (112.50, 81.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (145.51, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 261.819 CPU sec, 8.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11509, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (146.05, 60.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 297.781 CPU sec, 10.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11493, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (146.43, 61.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 325.790 CPU sec, 11.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12094, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (142.68, 57.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 246.179 CPU sec, 8.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12694, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (143.69, 57.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 213.146 CPU sec, 7.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12278, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (159.92, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (142.07, 56.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.062 CPU sec, 7.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 10743, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 83, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (125.46, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (144.71, 59.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 355.528 CPU sec, 12.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10950, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.95, 57.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 322.408 CPU sec, 11.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11182, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 76, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (84.17, 47.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (143.04, 59.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 293.473 CPU sec, 9.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11471, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (149.44, 65.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (144.09, 59.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.749 CPU sec, 9.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 10878, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (146.29, 59.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 286.192 CPU sec, 10.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11025, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 126, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (127.00, 45.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (145.96, 60.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 336.115 CPU sec, 11.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11326, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.15, 60.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 323.222 CPU sec, 11.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12684, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (107.38, 47.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (142.85, 56.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.723 CPU sec, 7.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12692, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 123, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (140.00, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (140.74, 54.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.333 CPU sec, 6.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11718, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 165, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (227.17, 194.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (143.86, 57.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 270.435 CPU sec, 9.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11040, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 81, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (97.30, 39.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (143.70, 57.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 336.553 CPU sec, 11.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 10974, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 105, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (132.82, 88.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.42, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 327.575 CPU sec, 11.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11438, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 120, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (127.30, 57.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (142.51, 57.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 266.055 CPU sec, 9.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11036, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (143.48, 58.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 280.587 CPU sec, 9.631 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 10816, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 204, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (158.11, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.63, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 327.835 CPU sec, 11.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 10746, 5, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (144.92, 58.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 336.815 CPU sec, 12.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12030, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (144.79, 59.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 264.068 CPU sec, 9.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12655, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 107, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (151.09, 117.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (142.17, 56.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.340 CPU sec, 7.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12526, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 135, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (136.45, 89.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (141.54, 55.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.957 CPU sec, 7.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 10948, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 93, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (106.15, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (146.64, 61.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 352.102 CPU sec, 13.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11062, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (145.16, 60.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 334.850 CPU sec, 11.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11389, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 103, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (147.73, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (143.67, 58.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 314.056 CPU sec, 10.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11398, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (143.24, 57.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 276.513 CPU sec, 9.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11219, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (132.33, 83.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (144.71, 59.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 286.066 CPU sec, 10.005 real sec [M::process] read 45542 sequences (6876842 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11169, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 90, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (106.09, 59.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 136, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (144.29, 58.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 327.328 CPU sec, 11.471 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 7576, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (145.20, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 45542 reads in 237.308 CPU sec, 8.111 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21177\tSM:UG10S1P041\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21177_HLKNYBGXX_UG10S1P041_R1.fastq.gz 5347_7332_21177_HLKNYBGXX_UG10S1P041_R2.fastq.gz [main] Real time: 2188.130 sec; CPU: 60899.451 sec Finished alignement of UG10S1P041 at Wed Apr 3 14:32:41 2019 Sorting BAM file started at Wed Apr 3 14:32:41 2019 samtools sort -@ 30 -m 4G UG10S1P041_unsorted.bam -o UG10S1P041.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 14:32:41 2019 rm UG10S1P041_unsorted.bam rm 5347_7332_21177_HLKNYBGXX_UG10S1P041_R1.fastq.gz rm 5347_7332_21177_HLKNYBGXX_UG10S1P041_R2.fastq.gz samtools index UG10S1P041.bam Finished BAM file generation of the sample - UG10S1P041 Processing fastq files for the sample - UG10S2P468 Reading 5347_7332_21178_HLKNYBGXX_UG10S2P468_R1.fastq.gz 5347_7332_21178_HLKNYBGXX_UG10S2P468_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21178\tSM:UG10S2P468\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21178_HLKNYBGXX_UG10S2P468_R1.fastq.gz 5347_7332_21178_HLKNYBGXX_UG10S2P468_R2.fastq.gz | samtools view -Sb1 - > UG10S2P468_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14400, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 158, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (205.09, 165.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.72, 100.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.483 CPU sec, 7.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14882, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 147, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (144.89, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (196.53, 103.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.713 CPU sec, 8.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14664, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (143.77, 89.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (197.01, 103.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.502 CPU sec, 7.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14684, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 172, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (198.35, 121.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (199.73, 105.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.206 CPU sec, 7.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14639, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 144, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (146.43, 106.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (199.28, 105.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.415 CPU sec, 8.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14638, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 149, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (159.68, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.09, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.807 CPU sec, 9.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14462, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 170, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (173.40, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (199.42, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.374 CPU sec, 8.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14447, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 98, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (102.36, 63.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (198.57, 103.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.134 CPU sec, 9.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14669, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 125, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (137.49, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (198.21, 104.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.751 CPU sec, 8.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14831, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (148.33, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (198.15, 103.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.555 CPU sec, 8.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14734, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 162, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (152.64, 91.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (197.14, 103.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.272 CPU sec, 7.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14793, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (134.14, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.71, 102.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.342 CPU sec, 8.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 15063, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 141, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (132.15, 83.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.10, 101.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.374 CPU sec, 8.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14678, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 123, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (119.69, 61.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (195.03, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.893 CPU sec, 8.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14577, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 130, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (152.72, 118.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (196.92, 102.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.914 CPU sec, 7.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14558, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (139.03, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.37, 103.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.034 CPU sec, 8.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14611, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 159, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (170.17, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (196.17, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.451 CPU sec, 8.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14868, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 132, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (135.17, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (199.06, 105.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.341 CPU sec, 8.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14777, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 138, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (165.00, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.64, 104.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.498 CPU sec, 8.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14835, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 106, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (133.11, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (201.12, 106.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.845 CPU sec, 8.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14605, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (114.50, 65.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (199.25, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.778 CPU sec, 7.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14722, 4, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (132.29, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (196.07, 102.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.097 CPU sec, 7.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14785, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (105.86, 48.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (198.63, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.276 CPU sec, 6.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14858, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (126.00, 89.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (194.99, 101.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.220 CPU sec, 6.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14819, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 94, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (100.00, 52.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (195.93, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.123 CPU sec, 6.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14975, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 93, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (109.58, 65.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (197.48, 102.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.168 CPU sec, 6.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14667, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 201, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (194.59, 105.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (195.69, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.236 CPU sec, 7.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14438, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 97, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (115.82, 65.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (192.87, 99.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.012 CPU sec, 8.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14730, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 111, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (124.08, 68.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (195.12, 100.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.178 CPU sec, 6.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14644, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 141, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (138.04, 60.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (198.77, 105.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.249 CPU sec, 7.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14864, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 171, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (191.05, 84.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (193.74, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.023 CPU sec, 6.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14544, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (196.68, 94.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (198.00, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.038 CPU sec, 7.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14630, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 121, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (163.64, 116.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (197.73, 104.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.842 CPU sec, 7.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14576, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 123, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (150.43, 99.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.47, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.212 CPU sec, 7.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14694, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (151.35, 109.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.85, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.888 CPU sec, 7.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14664, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 176, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (153.27, 89.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (197.39, 103.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.194 CPU sec, 8.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14870, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (123.61, 59.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.54, 102.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.883 CPU sec, 7.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14824, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 129, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (136.06, 78.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.46, 99.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.060 CPU sec, 7.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14890, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 156, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (177.67, 127.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (195.01, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.735 CPU sec, 7.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14912, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (132.41, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.84, 102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.183 CPU sec, 8.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14616, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 80, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (111.03, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (190.94, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.101 CPU sec, 8.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14734, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 144, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (161.89, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (195.37, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.584 CPU sec, 9.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14492, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (116.94, 61.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.28, 103.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.303 CPU sec, 8.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14840, 2, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (131.70, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.58, 103.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.879 CPU sec, 8.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14689, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 143, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (158.95, 92.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.97, 104.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.421 CPU sec, 8.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14562, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 97, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (102.96, 47.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.96, 104.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.097 CPU sec, 9.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14515, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 154, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (133.23, 63.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.57, 104.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 257.054 CPU sec, 9.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14470, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 150, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (151.40, 104.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.61, 102.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.666 CPU sec, 8.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14521, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 84, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (140.95, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (195.99, 102.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.423 CPU sec, 9.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14710, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 122, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (135.25, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (192.24, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.760 CPU sec, 8.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14762, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (144.83, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (192.67, 97.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.291 CPU sec, 8.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14831, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 117, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (118.56, 65.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (195.98, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.750 CPU sec, 8.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14587, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (129.90, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (193.84, 98.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.436 CPU sec, 7.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14470, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 124, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (126.95, 46.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (191.05, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.382 CPU sec, 8.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14567, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 126, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (152.07, 102.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.74, 98.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.324 CPU sec, 8.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14532, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (113.70, 73.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.87, 100.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.261 CPU sec, 8.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14717, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (148.64, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (197.70, 104.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.391 CPU sec, 9.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14736, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (132.79, 82.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (193.62, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.780 CPU sec, 8.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14536, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 149, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (160.67, 92.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (195.88, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.412 CPU sec, 8.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14634, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 120, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (137.79, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.15, 104.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.430 CPU sec, 8.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14581, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 123, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (122.52, 65.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (195.46, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.590 CPU sec, 9.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14534, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (130.67, 78.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.98, 103.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.174 CPU sec, 8.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14727, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 130, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (157.74, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (194.42, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.247 CPU sec, 8.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14718, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 93, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (108.37, 62.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.95, 100.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.284 CPU sec, 8.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14875, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 152, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (167.93, 96.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (192.79, 98.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.901 CPU sec, 7.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14834, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 149, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (145.21, 86.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.02, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.087 CPU sec, 7.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14757, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 137, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (146.53, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (191.99, 97.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.879 CPU sec, 7.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14574, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 97, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (95.89, 38.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (192.91, 98.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.767 CPU sec, 10.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14626, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 122, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (153.71, 102.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.84, 99.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.943 CPU sec, 8.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14642, 5, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 141, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (151.08, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.27, 99.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.031 CPU sec, 8.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14505, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 120, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (129.22, 65.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (194.30, 101.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 257.709 CPU sec, 9.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14488, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (129.62, 63.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (195.78, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.950 CPU sec, 8.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14439, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 118, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (137.88, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (195.71, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.005 CPU sec, 8.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14454, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 142, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (159.71, 90.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (194.20, 100.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.798 CPU sec, 9.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14594, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 121, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (130.72, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (195.92, 102.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.919 CPU sec, 7.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14609, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 147, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (143.29, 83.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 167, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (194.57, 99.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.630 CPU sec, 8.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14728, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 124, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (164.37, 109.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.79, 100.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.132 CPU sec, 8.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14929, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 108, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (147.84, 118.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (192.30, 98.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.136 CPU sec, 7.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14789, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (141.69, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (189.43, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.020 CPU sec, 7.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14743, 6, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 99, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (116.50, 89.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (190.70, 96.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.255 CPU sec, 8.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14564, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 106, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (134.85, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (200.81, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.053 CPU sec, 7.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14655, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (151.23, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (200.30, 106.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.508 CPU sec, 8.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14490, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (160.56, 82.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (197.96, 104.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.357 CPU sec, 8.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14546, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 117, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (131.64, 79.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.19, 103.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.831 CPU sec, 8.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14624, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 115, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (99.22, 42.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (199.30, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.016 CPU sec, 9.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14498, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 109, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (121.85, 62.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.09, 104.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.142 CPU sec, 8.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14683, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 138, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (133.69, 71.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (200.61, 106.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.923 CPU sec, 8.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14717, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 88, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (100.88, 58.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (196.77, 102.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.707 CPU sec, 8.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14684, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (130.23, 81.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (195.65, 102.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.435 CPU sec, 7.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14943, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (125.44, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.79, 103.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.274 CPU sec, 8.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14619, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (127.09, 66.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (193.00, 98.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.714 CPU sec, 8.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14712, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (150.59, 91.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (193.36, 98.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.805 CPU sec, 8.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14261, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 98, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (116.09, 72.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (189.31, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.006 CPU sec, 8.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14336, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 138, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (134.58, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (197.14, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.366 CPU sec, 8.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14589, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 142, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (166.38, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (202.02, 107.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.825 CPU sec, 7.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14711, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 121, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (135.54, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (200.82, 106.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.011 CPU sec, 7.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14652, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (158.36, 135.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (201.98, 108.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.646 CPU sec, 9.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14674, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (174.27, 103.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (201.37, 107.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.481 CPU sec, 8.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14548, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 105, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (112.90, 56.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (203.57, 109.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 257.228 CPU sec, 9.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14815, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 113, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (160.39, 115.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (198.11, 104.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.469 CPU sec, 8.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14472, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (147.41, 75.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (200.90, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.940 CPU sec, 8.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14893, 7, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 135, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (158.18, 120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (199.71, 106.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.997 CPU sec, 8.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14769, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (151.36, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (197.67, 103.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.571 CPU sec, 8.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14906, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (135.45, 81.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (192.81, 99.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.011 CPU sec, 8.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14782, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (142.73, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.93, 99.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.568 CPU sec, 8.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14565, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (136.94, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.95, 94.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.603 CPU sec, 8.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14413, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (181.30, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (191.22, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.753 CPU sec, 8.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14417, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (113.58, 61.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (126, 173, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (203.72, 108.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.218 CPU sec, 8.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14685, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 120, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (123.82, 45.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (202.17, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.203 CPU sec, 8.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14514, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (171.25, 151.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (202.57, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.236 CPU sec, 9.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14532, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 156, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (146.96, 81.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 171, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (201.56, 107.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.264 CPU sec, 8.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14466, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (178.93, 116.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (200.83, 106.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.947 CPU sec, 8.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14581, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (119.82, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.52, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.692 CPU sec, 8.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14714, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 117, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.13, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.69, 103.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.638 CPU sec, 8.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14651, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 148, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (142.96, 100.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.84, 102.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.937 CPU sec, 8.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 15008, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 147, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (201.03, 184.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.56, 103.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.041 CPU sec, 8.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14732, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 185, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (161.44, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (193.00, 98.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.944 CPU sec, 8.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14858, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (155.00, 118.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (191.79, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.353 CPU sec, 8.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14478, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 140, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (149.73, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.61, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.476 CPU sec, 8.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14291, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (143.72, 88.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.87, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.337 CPU sec, 8.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14476, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 115, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (131.12, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.42, 102.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.964 CPU sec, 8.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14581, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 166, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (174.90, 111.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (196.72, 102.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.640 CPU sec, 8.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14544, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 150, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (173.22, 108.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (195.49, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.668 CPU sec, 8.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14406, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 136, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (158.57, 93.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.66, 102.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.524 CPU sec, 8.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14483, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (144.91, 83.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (199.17, 104.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 260.485 CPU sec, 9.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14472, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 93, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (97.88, 59.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (199.59, 105.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.046 CPU sec, 9.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14661, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (129.61, 77.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.73, 105.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.876 CPU sec, 8.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14600, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 129, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (160.27, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.94, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.322 CPU sec, 8.674 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14551, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (159.40, 98.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (195.25, 100.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.206 CPU sec, 8.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14679, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 137, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (159.00, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (193.72, 99.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.510 CPU sec, 8.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14865, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 126, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (128.44, 56.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (190.40, 95.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.871 CPU sec, 7.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14540, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 124, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (127.79, 93.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (188.82, 94.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.495 CPU sec, 7.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14404, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 124, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (138.18, 93.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (184.70, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.418 CPU sec, 8.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14471, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 130, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (144.74, 94.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (194.93, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.536 CPU sec, 7.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14516, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 97, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (112.17, 67.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (199.35, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.195 CPU sec, 8.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14517, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 134, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (143.58, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (201.15, 105.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.489 CPU sec, 8.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14523, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 138, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (158.37, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (198.28, 103.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.296 CPU sec, 8.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14578, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 128, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (157.76, 133.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (200.98, 107.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.705 CPU sec, 8.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14653, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 138, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (179.04, 115.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (198.26, 105.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.382 CPU sec, 9.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14536, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (136.69, 65.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (200.11, 106.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.656 CPU sec, 8.766 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14703, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 104, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (131.11, 67.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.78, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.946 CPU sec, 8.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14620, 7, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 122, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (143.21, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (195.69, 102.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.852 CPU sec, 8.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14790, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (115.86, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (194.59, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.281 CPU sec, 8.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14908, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 119, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (135.18, 73.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (190.73, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.693 CPU sec, 7.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14569, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 101, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (112.81, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (188.47, 94.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.078 CPU sec, 7.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14537, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 126, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (126.61, 69.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (184.20, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.522 CPU sec, 8.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14372, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (115.73, 73.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (195.06, 100.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.441 CPU sec, 8.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14531, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (120.46, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.65, 104.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.146 CPU sec, 8.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14401, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (132.16, 50.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.80, 104.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.668 CPU sec, 9.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14379, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 126, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (153.59, 107.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.90, 104.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.285 CPU sec, 8.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14505, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 126, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (134.76, 69.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.24, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.437 CPU sec, 8.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14409, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 98, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (95.62, 47.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (199.34, 105.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.459 CPU sec, 8.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14641, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 97, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (119.11, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.59, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.533 CPU sec, 8.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14488, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 144, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (184.54, 133.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.73, 100.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.442 CPU sec, 9.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14526, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (95.42, 65.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (193.88, 100.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.874 CPU sec, 8.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14616, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 122, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (164.00, 121.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.06, 99.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.594 CPU sec, 8.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14718, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 95, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (109.84, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (191.99, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.601 CPU sec, 7.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 14440, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 110, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (149.36, 105.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (187.38, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.576 CPU sec, 7.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14472, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (143.50, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (184.33, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.552 CPU sec, 8.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14518, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 115, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (118.62, 61.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (191.01, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.927 CPU sec, 8.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14833, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 99, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (113.48, 72.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.27, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.860 CPU sec, 8.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14935, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 123, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (136.43, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.50, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.003 CPU sec, 7.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14840, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (149.50, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.90, 99.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.603 CPU sec, 7.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14953, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 139, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (131.00, 56.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (195.40, 100.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.467 CPU sec, 8.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14614, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 138, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (168.25, 115.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.61, 100.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.323 CPU sec, 8.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14626, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 97, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (104.41, 55.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (196.19, 101.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.291 CPU sec, 9.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14736, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 112, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (122.55, 75.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (195.60, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.499 CPU sec, 8.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14839, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 139, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (152.00, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (195.77, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.178 CPU sec, 7.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14506, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 132, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (133.33, 66.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (195.05, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.967 CPU sec, 8.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14764, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (142.30, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.11, 99.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.215 CPU sec, 9.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14704, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (156.22, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.85, 99.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.608 CPU sec, 8.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14770, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 138, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (140.38, 67.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.60, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.845 CPU sec, 8.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14925, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 186, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (168.56, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (197.24, 103.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.804 CPU sec, 8.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14983, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 101, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (111.55, 55.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (196.40, 101.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.490 CPU sec, 7.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14742, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 134, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (161.97, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (194.47, 100.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.303 CPU sec, 8.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14906, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 135, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (162.85, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (193.15, 98.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.696 CPU sec, 7.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 14777, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 119, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (141.42, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (197.35, 103.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.136 CPU sec, 7.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14714, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 92, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (123.71, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (197.72, 103.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.439 CPU sec, 8.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14719, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (132.12, 67.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (198.61, 103.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.000 CPU sec, 8.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14778, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (120.33, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.36, 102.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.765 CPU sec, 9.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14758, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 106, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (117.67, 68.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (200.25, 105.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.111 CPU sec, 8.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14377, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (132.19, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (193.01, 98.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 265.208 CPU sec, 10.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14609, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 84, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (100.39, 58.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (193.12, 99.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.858 CPU sec, 8.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14916, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (131.61, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.63, 101.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.968 CPU sec, 8.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14789, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 99, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (125.93, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (196.82, 102.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.155 CPU sec, 8.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 14960, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (148.28, 101.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (197.67, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.828 CPU sec, 7.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14884, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 162, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (156.18, 85.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.81, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.698 CPU sec, 7.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 15067, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (114.47, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.90, 100.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.851 CPU sec, 6.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14851, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 111, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (116.11, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.74, 95.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.653 CPU sec, 7.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14749, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 136, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (147.65, 83.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (199.07, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.766 CPU sec, 7.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14674, 11, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 142, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (147.04, 79.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.41, 104.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (566, 1923, 2954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7730) [M::mem_pestat] mean and std.dev: (2159.18, 2177.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 244.413 CPU sec, 8.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14740, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (136.04, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (198.31, 103.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.029 CPU sec, 9.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14746, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (175.29, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (194.99, 100.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.863 CPU sec, 8.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14512, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 128, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (138.06, 94.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (195.02, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.248 CPU sec, 8.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14565, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 80, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (94.64, 47.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (194.20, 99.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.168 CPU sec, 8.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 14551, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (135.23, 94.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.96, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.302 CPU sec, 9.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14807, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 142, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (133.96, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (196.89, 102.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.037 CPU sec, 8.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14861, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 121, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (147.25, 82.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.62, 104.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.590 CPU sec, 8.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14716, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (136.54, 60.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (193.04, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.607 CPU sec, 7.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14782, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 114, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (125.03, 86.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (191.54, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.086 CPU sec, 7.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14891, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 117, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (118.09, 69.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (193.05, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.453 CPU sec, 7.994 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14834, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (120.11, 69.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (191.43, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.951 CPU sec, 7.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14627, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 131, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (162.58, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.15, 99.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.639 CPU sec, 9.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14589, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 135, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (153.10, 107.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (196.60, 101.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.490 CPU sec, 8.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14545, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 159, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (175.16, 141.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (197.14, 102.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.914 CPU sec, 8.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14608, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 148, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (163.41, 101.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (194.16, 99.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.388 CPU sec, 8.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14520, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 138, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (141.18, 87.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (192.36, 98.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.169 CPU sec, 8.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14372, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 116, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (162.87, 116.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.89, 93.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.018 CPU sec, 8.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14468, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (144.07, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (193.56, 99.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.279 CPU sec, 8.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14511, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 107, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (132.90, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.91, 100.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.765 CPU sec, 8.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14681, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 128, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (123.94, 85.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (194.20, 101.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.256 CPU sec, 8.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 15005, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (183.28, 105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (195.14, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.786 CPU sec, 8.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14828, 9, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 90, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (104.37, 62.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.51, 98.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.185 CPU sec, 7.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14766, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 103, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (122.64, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (191.57, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.490 CPU sec, 8.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14616, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 116, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (143.23, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (192.96, 98.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.515 CPU sec, 8.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14698, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (150.03, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.43, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.157 CPU sec, 8.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14541, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 129, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (157.08, 104.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (197.03, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.527 CPU sec, 9.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14714, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 141, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (136.15, 86.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (199.15, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.891 CPU sec, 8.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14756, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 98, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (138.17, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (196.96, 103.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.079 CPU sec, 8.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14584, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 134, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (172.94, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (191.27, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.510 CPU sec, 8.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14602, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 101, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (110.71, 60.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (189.08, 95.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.126 CPU sec, 7.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14700, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (128.31, 75.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (191.89, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.202 CPU sec, 8.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14750, 4, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 139, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (146.14, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (194.69, 101.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.794 CPU sec, 8.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14719, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (139.50, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (193.73, 100.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.830 CPU sec, 8.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14819, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 97, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (126.00, 95.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (190.84, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.805 CPU sec, 7.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 14779, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 130, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (154.40, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (192.57, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.079 CPU sec, 8.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14748, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 92, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (123.40, 74.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (190.43, 96.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.642 CPU sec, 8.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14702, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (140.67, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (191.52, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.469 CPU sec, 7.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14777, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 134, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (167.42, 101.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (196.41, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.773 CPU sec, 8.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14535, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 101, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (124.08, 77.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (195.48, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.180 CPU sec, 8.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14549, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 136, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (150.58, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (197.91, 104.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.102 CPU sec, 8.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14568, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 125, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (112.46, 57.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (193.00, 98.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.117 CPU sec, 8.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14651, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (122.73, 57.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (191.60, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.121 CPU sec, 8.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14723, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 102, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (117.47, 75.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (190.29, 96.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.785 CPU sec, 8.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14684, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 114, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (134.35, 90.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (194.03, 100.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.082 CPU sec, 9.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14794, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 119, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (146.23, 101.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.49, 99.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.778 CPU sec, 8.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14873, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 154, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (156.00, 89.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (192.72, 99.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.963 CPU sec, 9.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14861, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 133, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (135.09, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (191.95, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.324 CPU sec, 7.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 14841, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 114, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (123.25, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (187.21, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.889 CPU sec, 7.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14692, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (170.68, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (188.96, 94.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.979 CPU sec, 8.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14347, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (124.74, 54.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (188.59, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.912 CPU sec, 8.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14649, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 123, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (145.23, 88.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.66, 98.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.928 CPU sec, 9.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14501, 4, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (131.68, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (198.30, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.055 CPU sec, 8.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14681, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 108, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (147.30, 110.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.69, 102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.119 CPU sec, 8.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 14725, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 114, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (146.82, 115.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (194.29, 100.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.334 CPU sec, 7.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 14823, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (122.21, 68.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (195.47, 100.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.521 CPU sec, 7.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14753, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 150, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (168.35, 108.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (197.14, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.290 CPU sec, 8.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14671, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 99, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (118.34, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (199.71, 105.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.262 CPU sec, 7.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14734, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 99, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (111.50, 61.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (198.19, 104.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.138 CPU sec, 7.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14627, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 101, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (151.17, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.20, 103.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.117 CPU sec, 8.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14697, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 119, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (143.96, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (193.18, 99.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.148 CPU sec, 8.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14768, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (138.62, 104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.82, 95.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.583 CPU sec, 7.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14717, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 123, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (169.52, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (189.88, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.654 CPU sec, 9.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14207, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 97, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (113.93, 84.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (187.77, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.245 CPU sec, 8.580 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14510, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 110, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (137.17, 91.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 172, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (199.23, 104.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.034 CPU sec, 8.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14620, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 135, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (153.27, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.16, 104.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.247 CPU sec, 8.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14731, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 105, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (160.28, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (197.70, 104.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.026 CPU sec, 9.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 102, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (127.00, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (195.85, 101.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.173 CPU sec, 8.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14609, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (142.62, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.19, 102.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.003 CPU sec, 8.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14468, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 142, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (125.83, 64.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (199.89, 106.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.989 CPU sec, 8.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14741, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 132, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (178.33, 132.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (199.89, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.645 CPU sec, 8.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14509, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 96, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (96.97, 40.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (198.60, 105.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.645 CPU sec, 8.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14381, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 95, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (131.65, 101.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (199.49, 104.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.110 CPU sec, 7.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14877, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 93, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (117.82, 85.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (192.81, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.657 CPU sec, 8.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14828, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (133.33, 65.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (190.15, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.686 CPU sec, 7.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 14925, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 129, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (139.25, 92.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (188.77, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.784 CPU sec, 7.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14441, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (165.79, 121.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (188.17, 93.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.934 CPU sec, 9.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14569, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (127.79, 70.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (197.62, 103.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.771 CPU sec, 8.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14624, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 123, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (139.38, 69.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.69, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.751 CPU sec, 8.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 14683, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 141, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (149.14, 88.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (195.91, 102.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.349 CPU sec, 8.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14707, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (143.14, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 167, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (195.85, 102.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.448 CPU sec, 8.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14806, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 115, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (136.89, 92.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (198.10, 104.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.407 CPU sec, 8.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14652, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 94, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (99.00, 51.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (198.48, 103.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.202 CPU sec, 9.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14603, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 93, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (113.00, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 171, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (200.03, 105.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.989 CPU sec, 8.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14747, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 94, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (117.40, 65.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (200.54, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.812 CPU sec, 9.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 14695, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 94, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (145.31, 106.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 168, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (195.78, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.765 CPU sec, 8.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14696, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (183.73, 137.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (190.70, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.913 CPU sec, 8.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14721, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (115.92, 65.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (189.09, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.242 CPU sec, 7.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14684, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (117.74, 66.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.79, 90.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.780 CPU sec, 8.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14174, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (108.48, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (182.66, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.046 CPU sec, 8.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14536, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 137, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (168.57, 116.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (125, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (196.20, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 264.617 CPU sec, 10.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14649, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 133, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (164.10, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (195.71, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.260 CPU sec, 8.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14767, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 95, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (113.06, 59.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.61, 99.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.954 CPU sec, 8.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14671, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (122.19, 71.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (190.44, 97.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.426 CPU sec, 8.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14652, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 100, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (143.44, 108.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (193.33, 99.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.878 CPU sec, 8.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14680, 1, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 136, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (134.08, 83.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.85, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.521 CPU sec, 8.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14644, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 117, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (124.58, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (196.68, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.541 CPU sec, 7.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14457, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 106, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (117.33, 63.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (198.62, 103.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.200 CPU sec, 8.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14579, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 119, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (117.04, 58.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.42, 99.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.511 CPU sec, 8.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14968, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 87, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (119.11, 88.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (190.31, 96.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.526 CPU sec, 7.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 14719, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 117, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (153.75, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (187.50, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.231 CPU sec, 8.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14319, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 89, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (76.48, 33.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 162, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (183.20, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.584 CPU sec, 8.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14245, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (155.87, 92.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (186.44, 91.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 255.949 CPU sec, 8.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14551, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 139, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (152.21, 110.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (197.74, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.244 CPU sec, 8.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14694, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (118.53, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (194.89, 100.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.490 CPU sec, 8.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14526, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 105, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (130.61, 102.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (193.69, 99.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.575 CPU sec, 8.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14747, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 118, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (127.61, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (194.19, 99.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.226 CPU sec, 8.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14748, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (159.56, 109.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (197.43, 103.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.579 CPU sec, 7.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14479, 7, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 111, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (135.07, 90.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (197.73, 104.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.210 CPU sec, 8.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14621, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 142, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (134.32, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (196.29, 101.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.672 CPU sec, 8.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14573, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (149.42, 102.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (194.08, 100.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.868 CPU sec, 8.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14689, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 160, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (175.50, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (194.98, 99.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.252 CPU sec, 8.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14698, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (134.90, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (191.46, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.303 CPU sec, 8.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14648, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 83, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (104.83, 66.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (186.67, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.635 CPU sec, 9.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14616, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 100, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (113.41, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (188.02, 93.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.173 CPU sec, 7.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14574, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 145, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (142.56, 80.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (187.50, 92.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.300 CPU sec, 8.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 14592, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 106, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (111.44, 65.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (197.53, 103.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.465 CPU sec, 9.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14645, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 122, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (117.17, 68.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 167, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (194.89, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.707 CPU sec, 7.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 14701, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 138, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (148.25, 68.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 170, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (196.02, 102.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.569 CPU sec, 9.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14672, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (149.40, 89.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (196.20, 100.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.071 CPU sec, 8.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14690, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (106.05, 60.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (122, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (195.90, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.174 CPU sec, 8.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14637, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (150.00, 78.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.46, 102.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.744 CPU sec, 8.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 14716, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (118.03, 61.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (196.19, 101.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.775 CPU sec, 8.333 real sec [M::process] read 30114 sequences (4547214 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 14618, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 91, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (112.97, 63.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 169, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (194.04, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.535 CPU sec, 8.341 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 6649, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 99, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (152.38, 106.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (191.06, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 30114 reads in 113.059 CPU sec, 4.429 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21178\tSM:UG10S2P468\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21178_HLKNYBGXX_UG10S2P468_R1.fastq.gz 5347_7332_21178_HLKNYBGXX_UG10S2P468_R2.fastq.gz [main] Real time: 2625.784 sec; CPU: 72158.102 sec Finished alignement of UG10S2P468 at Wed Apr 3 15:10:34 2019 Sorting BAM file started at Wed Apr 3 15:10:34 2019 samtools sort -@ 30 -m 4G UG10S2P468_unsorted.bam -o UG10S2P468.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 15:10:34 2019 rm UG10S2P468_unsorted.bam rm 5347_7332_21178_HLKNYBGXX_UG10S2P468_R1.fastq.gz rm 5347_7332_21178_HLKNYBGXX_UG10S2P468_R2.fastq.gz samtools index UG10S2P468.bam Finished BAM file generation of the sample - UG10S2P468 Processing fastq files for the sample - UG10S2P481 Reading 5347_7332_21179_HLKNYBGXX_UG10S2P481_R1.fastq.gz 5347_7332_21179_HLKNYBGXX_UG10S2P481_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21179\tSM:UG10S2P481\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21179_HLKNYBGXX_UG10S2P481_R1.fastq.gz 5347_7332_21179_HLKNYBGXX_UG10S2P481_R2.fastq.gz | samtools view -Sb1 - > UG10S2P481_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12963, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.43, 111.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.749 CPU sec, 7.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13166, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (218.38, 107.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.99, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.520 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13444, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 112, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (121.50, 39.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (222.49, 114.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.288 CPU sec, 5.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13449, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 160, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (168.60, 71.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (223.12, 115.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.315 CPU sec, 5.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13352, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 172, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (181.71, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (222.69, 113.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.803 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13266, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 166, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (161.29, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (222.83, 113.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.465 CPU sec, 5.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13458, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 150, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (172.20, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (221.91, 113.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.092 CPU sec, 6.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13469, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 181, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (222.92, 158.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.89, 111.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.724 CPU sec, 4.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13354, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 164, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (154.00, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (217.89, 109.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.700 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13477, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 130, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (124.78, 64.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (216.46, 109.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.745 CPU sec, 5.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13435, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (124.35, 69.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (216.44, 108.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.091 CPU sec, 4.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13347, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 143, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (158.04, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (217.01, 110.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.736 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12949, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 163, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (168.77, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (217.58, 109.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.802 CPU sec, 6.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13103, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 219, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (203.50, 86.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (218.18, 110.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.021 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13176, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 129, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (131.77, 81.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (218.69, 110.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.738 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13313, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 210, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (196.53, 112.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (221.79, 113.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.758 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13450, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 151, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (167.89, 108.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (221.53, 114.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.857 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13499, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 214, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (179.20, 83.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (220.96, 112.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.075 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13245, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (214.67, 171.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (221.04, 112.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.322 CPU sec, 4.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13421, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 107, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (143.11, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.71, 111.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.190 CPU sec, 5.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13352, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 127, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (145.80, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (219.49, 111.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.342 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13297, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 145, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (133.50, 68.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (216.63, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.164 CPU sec, 5.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13485, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 129, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (155.16, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.55, 108.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.500 CPU sec, 6.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13289, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 101, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (93.46, 34.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.70, 108.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.742 CPU sec, 6.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13090, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 156, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (183.19, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (218.13, 108.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.242 CPU sec, 6.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13198, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 171, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (166.35, 105.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.45, 110.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.647 CPU sec, 5.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13103, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 155, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.81, 82.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (220.28, 112.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.194 CPU sec, 5.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13240, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 121, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (141.20, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (219.51, 111.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.450 CPU sec, 6.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13248, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (161.30, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (221.74, 114.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.192 CPU sec, 5.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13130, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 90, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (109.84, 83.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (220.08, 111.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.924 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13393, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 135, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (144.55, 85.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.16, 111.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.498 CPU sec, 6.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13392, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (131.22, 79.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (217.15, 110.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.294 CPU sec, 4.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13233, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (138.22, 81.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (217.38, 111.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.614 CPU sec, 6.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13286, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 189, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (164.31, 89.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (215.61, 108.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.285 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13235, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 136, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (158.00, 95.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (216.19, 108.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.306 CPU sec, 6.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13110, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (183.33, 102.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (214.79, 108.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.637 CPU sec, 7.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13233, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (136.45, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.14, 108.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.067 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13152, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 146, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (178.36, 114.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 182, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (213.80, 109.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.612 CPU sec, 7.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13368, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 181, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (189.24, 100.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (218.09, 110.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.226 CPU sec, 6.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13286, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 183, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (191.71, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (219.43, 112.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.233 CPU sec, 5.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13232, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 166, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (163.94, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (221.33, 112.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.295 CPU sec, 5.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13337, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 137, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (136.94, 67.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (220.09, 112.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.750 CPU sec, 4.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13312, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (162.14, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (217.63, 110.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.247 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13354, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 153, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (141.68, 65.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (217.80, 111.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.743 CPU sec, 5.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13299, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 157, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (149.69, 81.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (214.24, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.157 CPU sec, 5.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13259, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 114, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (134.75, 101.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (217.27, 108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.740 CPU sec, 6.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13115, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 122, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (143.50, 87.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (214.82, 108.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.891 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13437, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 189, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (186.20, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (214.75, 107.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.207 CPU sec, 6.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13103, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 144, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (180.95, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (211.68, 105.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.718 CPU sec, 7.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12920, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 117, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (134.67, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (214.95, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.215 CPU sec, 6.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13290, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 152, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (146.50, 88.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (214.46, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.711 CPU sec, 4.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13091, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (170.59, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (217.80, 110.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.376 CPU sec, 4.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13338, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 137, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (170.32, 95.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (217.96, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.425 CPU sec, 4.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13331, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (145.95, 86.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (217.24, 109.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.971 CPU sec, 5.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13519, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 142, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (153.54, 94.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (217.53, 110.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.336 CPU sec, 4.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13297, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 156, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (162.24, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (217.94, 110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.179 CPU sec, 4.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13322, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 146, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (145.86, 67.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.33, 110.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.409 CPU sec, 5.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13292, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 118, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (145.05, 76.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (215.98, 108.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.236 CPU sec, 4.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13114, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 141, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (134.89, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (214.36, 107.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.872 CPU sec, 5.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13241, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 120, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (148.80, 90.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (213.85, 106.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.967 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13083, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 114, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (105.25, 49.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.78, 108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.465 CPU sec, 4.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12722, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 181, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (167.18, 106.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (212.31, 105.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.653 CPU sec, 5.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13061, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 187, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (183.00, 81.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (216.83, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.721 CPU sec, 5.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13290, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 197, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (202.05, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (218.73, 110.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.428 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13147, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 80, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (72.75, 25.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (216.80, 110.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.486 CPU sec, 4.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13079, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 131, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (152.20, 101.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (218.91, 111.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.794 CPU sec, 5.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13241, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 142, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (126.35, 50.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.79, 111.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.170 CPU sec, 5.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13164, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (142.18, 84.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (214.86, 107.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.007 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13111, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 122, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (161.11, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (216.59, 109.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.114 CPU sec, 4.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13219, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 146, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (162.00, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (213.24, 107.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.497 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13212, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (168.83, 107.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (214.96, 108.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.573 CPU sec, 5.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13263, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 141, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (141.62, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (212.81, 106.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.029 CPU sec, 4.543 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13207, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 161, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (162.44, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (213.16, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.263 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13034, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 125, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (124.31, 36.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (221.80, 113.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.835 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13289, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 123, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (144.83, 94.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (222.55, 114.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.739 CPU sec, 4.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13413, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 100, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (124.53, 78.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (221.49, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.679 CPU sec, 4.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13176, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (181.47, 144.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (222.07, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.736 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13135, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (163.06, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (222.05, 114.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.129 CPU sec, 5.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13499, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 168, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (173.56, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (221.87, 113.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.548 CPU sec, 5.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13243, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 187, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (194.14, 116.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (219.68, 111.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.861 CPU sec, 6.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13411, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 150, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (145.69, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (219.33, 111.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.616 CPU sec, 5.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13291, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (157.76, 123.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (215.66, 109.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.974 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13085, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 194, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (154.85, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 182, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (212.85, 106.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.387 CPU sec, 5.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13034, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 203, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (215.88, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (212.20, 105.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.565 CPU sec, 5.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12601, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 134, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (179.81, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (207.46, 101.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.773 CPU sec, 5.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13245, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 172, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (186.11, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 191, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (222.63, 113.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.666 CPU sec, 5.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13322, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 155, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (158.40, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (223.87, 114.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.999 CPU sec, 5.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13274, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 126, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (153.96, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 190, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (224.10, 115.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.012 CPU sec, 5.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13152, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 142, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (161.65, 83.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (223.85, 114.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.531 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13258, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 145, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (165.50, 106.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 192, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (224.12, 113.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.213 CPU sec, 7.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13387, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 165, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (215.36, 201.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (223.54, 114.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.032 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13412, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 136, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (163.15, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 190, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (221.63, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.169 CPU sec, 5.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13452, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 155, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (173.40, 106.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (219.67, 112.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.678 CPU sec, 7.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13253, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (140.38, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (217.72, 110.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.557 CPU sec, 6.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13136, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 89, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (148.64, 112.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 181, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (211.56, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.129 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12864, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (160.12, 68.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (213.12, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.231 CPU sec, 5.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12618, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 212, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (188.56, 83.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (209.04, 102.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.288 CPU sec, 5.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13136, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (156.56, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (222.51, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.227 CPU sec, 6.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13209, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 206, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (193.00, 89.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 191, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (224.81, 114.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.363 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13351, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 112, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (126.79, 61.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 190, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (223.14, 114.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.269 CPU sec, 5.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13384, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 231, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (248.31, 163.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (222.09, 113.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.376 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13314, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (165.47, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (221.47, 113.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.527 CPU sec, 5.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13349, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (183.42, 116.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (222.55, 113.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.748 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13288, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 181, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (183.88, 128.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (221.67, 114.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.757 CPU sec, 5.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13318, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (137.68, 92.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (218.09, 110.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.352 CPU sec, 6.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13388, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 130, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (135.11, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 181, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (213.14, 107.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.679 CPU sec, 5.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13111, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 120, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (99.62, 34.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (213.33, 108.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.535 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13057, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 169, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.19, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (210.10, 105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.057 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12512, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 138, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (150.53, 77.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (208.78, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.344 CPU sec, 6.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13039, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (119.92, 63.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (219.30, 110.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.163 CPU sec, 5.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13219, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 132, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (164.85, 113.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (217.61, 111.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.210 CPU sec, 5.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13135, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 155, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (155.14, 85.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (219.75, 112.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.752 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13166, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 141, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (191.29, 144.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 187, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (219.36, 111.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.539 CPU sec, 5.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13113, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 186, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (202.44, 133.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (218.28, 111.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.543 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13343, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 151, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (165.81, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (218.00, 110.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.630 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13263, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 155, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (176.95, 122.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (218.83, 112.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.553 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13306, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 118, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (140.54, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (216.69, 108.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.135 CPU sec, 5.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13245, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 166, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (139.85, 81.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (214.21, 107.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.194 CPU sec, 6.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12997, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 149, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (171.87, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 182, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (211.73, 105.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.777 CPU sec, 5.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12907, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (154.67, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (209.65, 104.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.949 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12711, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 207, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (189.96, 113.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 177, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (204.09, 98.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.450 CPU sec, 5.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13060, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 154, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (176.96, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (217.59, 109.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.747 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13402, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (153.62, 103.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 188, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (221.67, 114.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.764 CPU sec, 5.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13466, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 165, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (161.29, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (221.60, 113.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.481 CPU sec, 5.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13173, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 130, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (135.83, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (220.83, 111.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.472 CPU sec, 6.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13376, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 196, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (175.94, 90.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (221.88, 112.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.200 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13359, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 121, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (134.61, 64.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (219.40, 112.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.268 CPU sec, 7.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13354, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 107, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (122.84, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (220.70, 113.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.334 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13328, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 159, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (180.50, 108.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (217.29, 109.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.066 CPU sec, 5.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13251, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 123, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (149.74, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (216.03, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.204 CPU sec, 5.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13116, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 125, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (142.10, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (213.07, 106.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.405 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12914, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 162, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (171.16, 93.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 182, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (210.83, 104.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.311 CPU sec, 5.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12751, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 177, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 379) [M::mem_pestat] mean and std.dev: (181.45, 79.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 178, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (205.59, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.575 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12817, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (126.53, 68.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (216.73, 108.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.803 CPU sec, 6.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13186, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (128.48, 71.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (221.94, 113.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.454 CPU sec, 5.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13234, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 128, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (119.67, 45.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (221.66, 114.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.033 CPU sec, 5.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13234, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 168, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (201.85, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (220.11, 111.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.747 CPU sec, 5.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13163, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 191, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (230.24, 186.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.37, 112.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.318 CPU sec, 5.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13101, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (129.54, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (220.41, 111.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.916 CPU sec, 6.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13282, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (144.82, 95.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (216.43, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.277 CPU sec, 5.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13232, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 144, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (189.04, 112.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (216.94, 110.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.520 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13277, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 135, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (164.95, 110.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (214.19, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.668 CPU sec, 6.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13125, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 101, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (168.42, 130.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (214.41, 108.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.480 CPU sec, 6.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13051, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 147, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (154.68, 84.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 178, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (208.73, 103.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.917 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12731, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 134, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (162.88, 93.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 175, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (202.80, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.185 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13070, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 137, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (137.31, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (211.52, 105.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.889 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13284, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 157, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (181.95, 110.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (214.30, 108.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.555 CPU sec, 5.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13325, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (151.37, 104.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (214.83, 108.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.969 CPU sec, 5.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13378, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 126, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (156.00, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (213.71, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.264 CPU sec, 6.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13221, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (181.00, 60.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 186, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (218.84, 112.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.998 CPU sec, 6.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13357, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 141, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (158.38, 91.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (219.59, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.160 CPU sec, 5.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13235, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 93, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (166.13, 125.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (218.99, 112.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.051 CPU sec, 4.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13265, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 83, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (120.79, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (216.86, 111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.604 CPU sec, 5.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12993, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 131, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (146.29, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (218.71, 110.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.379 CPU sec, 5.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13074, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 168, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (224.88, 167.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (217.05, 110.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.283 CPU sec, 5.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13052, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 165, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (181.26, 108.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (218.59, 111.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.269 CPU sec, 5.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13364, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 164, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (170.12, 98.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (221.90, 112.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.509 CPU sec, 6.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13435, 0, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 195, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (182.56, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (216.39, 110.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.194 CPU sec, 5.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13237, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 170, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (174.59, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (217.09, 109.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.676 CPU sec, 5.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13284, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 134, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (146.53, 76.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (213.53, 107.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.678 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13217, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 152, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (158.92, 95.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (218.11, 109.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.519 CPU sec, 6.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13280, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 153, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (168.55, 97.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (222.57, 113.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.945 CPU sec, 5.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13283, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 121, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (123.29, 67.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (218.91, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.955 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13281, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 136, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (146.06, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.70, 111.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.583 CPU sec, 5.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13256, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 199, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (179.13, 109.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (218.59, 110.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.623 CPU sec, 7.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12837, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (140.47, 60.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (216.03, 108.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.901 CPU sec, 6.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13247, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 93, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (138.73, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (217.80, 110.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.556 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13126, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 135, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (162.29, 107.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (215.99, 109.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.247 CPU sec, 6.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13283, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 147, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (149.25, 76.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (218.70, 111.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.026 CPU sec, 6.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13364, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 187, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (176.53, 69.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (213.60, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.917 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13084, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 98, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (95.00, 38.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (213.18, 106.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.797 CPU sec, 5.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13237, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 144, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (139.91, 68.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (210.42, 104.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.594 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13347, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 119, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (151.04, 88.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (218.45, 110.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.342 CPU sec, 5.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13189, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 142, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (175.81, 118.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (219.40, 112.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.072 CPU sec, 6.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13273, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 137, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (163.10, 99.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (219.12, 111.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.089 CPU sec, 6.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13418, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 149, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (137.65, 57.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (215.56, 109.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.415 CPU sec, 5.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13008, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 203, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (195.87, 113.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (216.44, 109.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.920 CPU sec, 6.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13006, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 150, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (183.87, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (218.17, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.578 CPU sec, 7.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13385, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (135.75, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.83, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.008 CPU sec, 6.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13400, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (157.73, 93.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (221.04, 112.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.075 CPU sec, 6.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13182, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (142.97, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.62, 107.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.452 CPU sec, 5.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13322, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 158, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (160.83, 132.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (214.26, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.040 CPU sec, 6.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13124, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 151, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (164.12, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (213.87, 107.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.786 CPU sec, 6.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13153, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (130.68, 78.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (213.09, 106.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.684 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13383, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 123, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (148.82, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.74, 111.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.352 CPU sec, 7.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13228, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 137, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (168.16, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (216.76, 108.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.126 CPU sec, 6.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13217, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (117.07, 80.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (216.30, 109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.158 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13151, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 140, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (147.22, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (216.43, 108.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.705 CPU sec, 6.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12806, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 160, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (170.61, 82.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 180, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (210.59, 104.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.555 CPU sec, 4.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13065, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 119, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (137.84, 65.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (212.95, 106.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.359 CPU sec, 6.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13248, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 163, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 283) [M::mem_pestat] mean and std.dev: (136.89, 39.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (215.96, 109.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.299 CPU sec, 5.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13292, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (199.77, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (217.47, 110.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.982 CPU sec, 5.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13362, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 117, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 182) [M::mem_pestat] mean and std.dev: (114.82, 14.37) [M::mem_pestat] low and high boundaries for proper pairs: (32, 207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (214.55, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.244 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13246, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 138, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (160.00, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (215.15, 108.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.638 CPU sec, 5.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13289, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 145, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (159.73, 100.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (211.33, 104.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.333 CPU sec, 5.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13365, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 144, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (172.13, 167.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (215.13, 107.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.346 CPU sec, 7.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13329, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 194, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (204.43, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (216.97, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.006 CPU sec, 6.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13371, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 142, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (137.58, 58.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (216.40, 109.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.572 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13397, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 215, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (192.60, 89.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (217.21, 109.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.631 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13155, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (134.62, 75.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 183, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (215.18, 108.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.811 CPU sec, 5.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12950, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 176, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (173.19, 98.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (209.19, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.405 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13122, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 188, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (177.36, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (212.35, 106.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.524 CPU sec, 6.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13139, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (128.19, 84.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (216.13, 109.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.950 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13252, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 107, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (137.81, 86.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (217.57, 110.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.713 CPU sec, 6.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13284, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 155, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (177.77, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 182, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (213.55, 106.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.531 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13212, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 150, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (159.45, 91.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (209.90, 104.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.767 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13176, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 131, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (135.47, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (209.85, 103.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.768 CPU sec, 5.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13219, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 176, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (200.04, 129.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (213.09, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.594 CPU sec, 6.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13297, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 153, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (152.17, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (217.43, 110.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.715 CPU sec, 5.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13281, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 146, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (143.29, 67.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (217.39, 109.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.912 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13269, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 160, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (171.79, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (214.14, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.061 CPU sec, 5.276 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13146, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 147, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (169.92, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (211.47, 105.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.810 CPU sec, 5.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13190, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 112, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (159.74, 110.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (213.07, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.502 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13124, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 194, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (187.71, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.31, 109.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.837 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13269, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 157, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (169.37, 96.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (215.18, 108.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.821 CPU sec, 6.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13160, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (168.79, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (216.21, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.524 CPU sec, 6.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13161, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 178, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (169.40, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (210.91, 105.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.957 CPU sec, 5.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12893, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 174, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (172.86, 85.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 178, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (207.44, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.659 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12766, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 107, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (131.89, 79.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (208.27, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.746 CPU sec, 6.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13300, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 166, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (182.21, 105.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (216.14, 109.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.043 CPU sec, 5.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13427, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (180.36, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (218.73, 112.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.348 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13538, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 107, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (110.63, 45.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (217.72, 110.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.415 CPU sec, 4.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13296, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (129.43, 47.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (215.85, 108.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.455 CPU sec, 6.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13456, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 75, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (105.37, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (219.42, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.835 CPU sec, 6.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13231, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (147.36, 89.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (221.63, 112.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.880 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13055, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 131, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (145.62, 91.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (223.18, 114.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.176 CPU sec, 6.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13302, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 216, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (172.39, 98.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (219.86, 112.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.886 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13288, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 186, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (160.95, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (213.58, 106.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.980 CPU sec, 5.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13036, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (139.59, 61.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 181, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (211.53, 106.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.200 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12820, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 169, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (156.71, 84.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 177, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (206.09, 101.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.375 CPU sec, 6.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12577, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 164, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (131.58, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (209.48, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.449 CPU sec, 6.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13404, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 146, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (138.71, 85.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (221.44, 112.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.922 CPU sec, 6.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13348, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 165, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (173.55, 93.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (218.66, 111.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.826 CPU sec, 6.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13428, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 211, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (215.24, 108.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (219.77, 110.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.527 CPU sec, 6.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13159, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 117, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 228) [M::mem_pestat] mean and std.dev: (104.39, 31.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (217.55, 111.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.665 CPU sec, 5.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13284, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 167, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (193.85, 104.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (221.09, 112.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.544 CPU sec, 8.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13098, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 172, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (202.76, 123.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 189, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (221.62, 112.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.548 CPU sec, 6.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13381, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (142.50, 96.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (221.31, 111.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.196 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13406, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 154, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (147.18, 69.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 192, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (223.15, 112.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.863 CPU sec, 6.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13317, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 164, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (149.50, 83.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (213.86, 107.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.478 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13220, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 122, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (123.10, 65.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 181, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (212.28, 107.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.806 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12959, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (137.21, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 181, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (211.02, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.030 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12919, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (110.47, 56.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (215.04, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.828 CPU sec, 6.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13520, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 246, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (231.44, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (221.05, 114.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.241 CPU sec, 5.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13448, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 151, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (142.74, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (217.69, 111.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.609 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13372, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 186, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (180.21, 73.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (217.37, 109.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.754 CPU sec, 6.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13179, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 144, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (174.89, 127.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (218.43, 111.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.575 CPU sec, 5.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13176, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (150.04, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 190, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (222.20, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.208 CPU sec, 5.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13394, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (116.07, 55.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (220.37, 112.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.919 CPU sec, 5.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13245, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 156, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (161.00, 76.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 189, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (222.27, 113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.164 CPU sec, 6.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13233, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 150, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (151.25, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.50, 108.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.430 CPU sec, 6.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13089, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 102, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (106.12, 50.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (211.43, 104.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.517 CPU sec, 6.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12947, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 162, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (158.36, 92.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 178, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (207.42, 102.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.677 CPU sec, 6.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12616, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 100, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (132.45, 71.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 176, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.86, 97.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.786 CPU sec, 6.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13146, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 97, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (131.47, 96.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 183, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (213.52, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.812 CPU sec, 6.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13419, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (164.16, 105.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (216.61, 110.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.775 CPU sec, 5.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13223, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 114, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (136.95, 64.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (216.07, 108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.372 CPU sec, 7.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13311, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (126.64, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (212.06, 106.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.689 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13287, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 194, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (160.56, 68.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (217.37, 111.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.434 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13181, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 112, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.65, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (218.96, 111.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.871 CPU sec, 6.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13141, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 135, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (146.45, 81.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (219.21, 111.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.164 CPU sec, 5.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13316, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.14, 94.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (220.02, 111.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.377 CPU sec, 6.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13258, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 122, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (146.14, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 182, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (211.45, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.439 CPU sec, 6.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12970, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 179, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (160.15, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (207.01, 101.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.861 CPU sec, 5.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12656, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 153, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (132.45, 58.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (202.69, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.249 CPU sec, 6.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12660, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 125, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (154.83, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 178, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (206.00, 100.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.233 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13255, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 174, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (160.58, 67.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 188, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (218.34, 110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.462 CPU sec, 5.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13500, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 158, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (117.56, 60.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 187, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (217.41, 110.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.175 CPU sec, 6.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13266, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 93, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (131.63, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (216.93, 110.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.479 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13308, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 165, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (172.81, 119.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (215.30, 107.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.128 CPU sec, 6.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13183, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 173, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (196.27, 110.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (218.51, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.556 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13385, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 152, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (179.00, 96.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (219.07, 110.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.499 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13357, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 217, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (233.17, 131.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (218.74, 111.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.231 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13350, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 97, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (81.56, 26.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 185, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (216.14, 108.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.903 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13244, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 177, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (178.72, 137.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.16, 103.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.985 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13296, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 130, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (153.62, 73.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (209.61, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.291 CPU sec, 6.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12979, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 128, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 277) [M::mem_pestat] mean and std.dev: (128.08, 42.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 179, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (207.37, 100.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.645 CPU sec, 5.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13038, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (141.40, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 180, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (212.01, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.888 CPU sec, 6.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13441, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 102, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (139.04, 85.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 186, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (218.27, 110.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.541 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13213, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 147, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (156.67, 112.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.32, 108.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.982 CPU sec, 6.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13412, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 118, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (128.62, 68.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (212.54, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.742 CPU sec, 5.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13233, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 112, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (136.71, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (215.40, 108.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.282 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13189, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 125, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (116.71, 49.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 187, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (217.85, 109.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.200 CPU sec, 5.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13243, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 95, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 186) [M::mem_pestat] mean and std.dev: (98.80, 26.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (218.63, 110.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.891 CPU sec, 6.033 real sec [M::process] read 24570 sequences (3710070 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13354, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (145.62, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (216.96, 110.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.415 CPU sec, 4.466 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 4932, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 99, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (134.91, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (215.97, 109.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 24570 reads in 57.448 CPU sec, 2.545 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21179\tSM:UG10S2P481\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21179_HLKNYBGXX_UG10S2P481_R1.fastq.gz 5347_7332_21179_HLKNYBGXX_UG10S2P481_R2.fastq.gz [main] Real time: 1650.664 sec; CPU: 41000.152 sec Finished alignement of UG10S2P481 at Wed Apr 3 15:56:10 2019 Sorting BAM file started at Wed Apr 3 15:56:10 2019 samtools sort -@ 30 -m 4G UG10S2P481_unsorted.bam -o UG10S2P481.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 15:56:10 2019 rm UG10S2P481_unsorted.bam rm 5347_7332_21179_HLKNYBGXX_UG10S2P481_R1.fastq.gz rm 5347_7332_21179_HLKNYBGXX_UG10S2P481_R2.fastq.gz samtools index UG10S2P481.bam Finished BAM file generation of the sample - UG10S2P481 Processing fastq files for the sample - UG10S2P486 Reading 5347_7332_21180_HLKNYBGXX_UG10S2P486_R1.fastq.gz 5347_7332_21180_HLKNYBGXX_UG10S2P486_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21180\tSM:UG10S2P486\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21180_HLKNYBGXX_UG10S2P486_R1.fastq.gz 5347_7332_21180_HLKNYBGXX_UG10S2P486_R2.fastq.gz | samtools view -Sb1 - > UG10S2P486_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12976, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 109, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (136.00, 78.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.99, 94.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.517 CPU sec, 8.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13088, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 127, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (132.18, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (190.00, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.267 CPU sec, 6.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13221, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 150, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (156.42, 80.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (188.02, 93.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.364 CPU sec, 6.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13350, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 118, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (151.56, 117.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.94, 96.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.694 CPU sec, 6.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13259, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 150, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (155.62, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (191.02, 97.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.391 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13409, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (159.71, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.71, 94.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.126 CPU sec, 6.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13363, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (134.18, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.12, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.548 CPU sec, 6.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13282, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (189.92, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.175 CPU sec, 6.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13257, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 142, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (145.95, 77.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (188.15, 94.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.446 CPU sec, 6.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13156, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 175, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (143.76, 104.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.42, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.657 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13180, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 175, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (170.50, 116.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.21, 92.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.386 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13326, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (143.10, 72.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.64, 91.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.187 CPU sec, 6.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13450, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 96, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (131.40, 77.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (184.31, 91.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.832 CPU sec, 6.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13443, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 161, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (161.90, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.61, 91.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.912 CPU sec, 7.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13435, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 162, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (145.46, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (187.94, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.330 CPU sec, 6.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13468, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 160, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (162.52, 91.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (184.92, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.626 CPU sec, 6.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13124, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (149.83, 66.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.90, 92.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.018 CPU sec, 6.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13036, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 245, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (196.91, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.17, 92.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.266 CPU sec, 7.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13058, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 92, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (135.82, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.40, 94.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.242 CPU sec, 7.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13321, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (137.71, 95.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.38, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.379 CPU sec, 6.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13305, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 191, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (165.53, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.95, 94.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.926 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13362, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 159, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (155.85, 80.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.83, 93.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.685 CPU sec, 6.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13345, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 150, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (155.35, 76.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (188.64, 95.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.839 CPU sec, 8.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13359, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 165, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (154.64, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (190.61, 96.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.036 CPU sec, 7.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13214, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 150, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (160.84, 69.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.30, 94.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.353 CPU sec, 6.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13212, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 130, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (135.47, 62.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.57, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.777 CPU sec, 6.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13295, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (142.62, 62.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (187.60, 94.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.547 CPU sec, 6.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13406, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 163, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (161.31, 73.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (188.23, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.577 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13362, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 103, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.94, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.83, 93.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.706 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13335, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (190.19, 141.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.89, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.993 CPU sec, 4.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13400, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 150, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (144.55, 79.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.10, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.276 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13454, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (167.11, 105.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.24, 91.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.597 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13436, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 160, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (159.88, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (184.07, 91.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.411 CPU sec, 4.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12984, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 115, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (126.38, 52.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (189.16, 95.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.540 CPU sec, 6.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13035, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.92, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.333 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13120, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (134.70, 52.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.60, 93.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.419 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13183, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 173, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (179.50, 96.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.17, 94.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.937 CPU sec, 4.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13084, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 128, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (135.65, 66.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (190.44, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.474 CPU sec, 4.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13162, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 200, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (169.50, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.55, 94.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.482 CPU sec, 5.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13146, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 172, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (158.56, 77.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (190.52, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.695 CPU sec, 6.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13253, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 138, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (141.37, 70.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.07, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.509 CPU sec, 6.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13141, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (143.67, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.87, 94.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.583 CPU sec, 6.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13247, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 99, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (131.71, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.77, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.035 CPU sec, 7.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13297, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 134, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (137.78, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.39, 92.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.400 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13376, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (124.93, 51.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.68, 93.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.741 CPU sec, 6.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13358, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 129, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (157.24, 103.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.63, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.797 CPU sec, 7.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13235, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (118.12, 59.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.33, 91.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.986 CPU sec, 5.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13264, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 117, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (117.38, 45.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.42, 90.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.639 CPU sec, 5.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13223, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 152, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (165.87, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (183.17, 88.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.937 CPU sec, 6.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13260, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (141.08, 77.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.36, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.663 CPU sec, 5.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13111, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (183.74, 116.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (185.00, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.715 CPU sec, 6.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13363, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 162, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (155.88, 99.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.39, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.070 CPU sec, 6.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13314, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 112, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (118.50, 64.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.63, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.521 CPU sec, 7.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13327, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (148.75, 90.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (187.41, 94.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.819 CPU sec, 6.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13113, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 239, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (185.83, 101.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.53, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.706 CPU sec, 6.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13420, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 155, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (165.36, 82.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (188.72, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.720 CPU sec, 6.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13334, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 102, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (91.22, 46.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.58, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.401 CPU sec, 7.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13237, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 117, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (136.95, 87.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.88, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.469 CPU sec, 6.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13244, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 97, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (110.86, 62.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.75, 93.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.628 CPU sec, 7.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13141, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 145, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (131.21, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.28, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.327 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13100, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (129.46, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.82, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.139 CPU sec, 6.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13324, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 91, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (106.58, 65.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.13, 92.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.675 CPU sec, 5.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13341, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 175, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (153.33, 78.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (184.91, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.198 CPU sec, 6.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13360, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (144.33, 59.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.28, 92.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.152 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13035, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (127.75, 77.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (183.94, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.723 CPU sec, 6.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13412, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (128.32, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (181.56, 88.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.449 CPU sec, 6.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13179, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 120, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (153.00, 86.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.52, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.182 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13118, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 165, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (184.38, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (181.68, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.823 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13177, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 153, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (145.10, 89.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.21, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.006 CPU sec, 6.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13324, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 113, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (123.50, 50.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.21, 91.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.407 CPU sec, 6.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13268, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (104.06, 43.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.30, 93.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.799 CPU sec, 6.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13388, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (69.67, 32.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.36, 92.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.974 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13345, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (167.69, 66.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.71, 92.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.771 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13126, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 171, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (166.00, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.40, 94.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.625 CPU sec, 6.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13114, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (135.77, 85.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (189.33, 94.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.385 CPU sec, 7.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13218, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 111, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (147.06, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.75, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.175 CPU sec, 6.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13263, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 83, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (123.35, 91.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.32, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.076 CPU sec, 5.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13273, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 194, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (195.67, 119.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.92, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.523 CPU sec, 6.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13305, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 137, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (151.07, 107.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.71, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.785 CPU sec, 6.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13456, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 139, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (149.86, 88.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (183.53, 89.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.784 CPU sec, 5.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13144, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 157, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (167.64, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.85, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.125 CPU sec, 7.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13252, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 140, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (134.93, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.67, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.421 CPU sec, 7.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13338, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (137.84, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.10, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.552 CPU sec, 6.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13103, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (126.09, 57.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (183.14, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.538 CPU sec, 6.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13084, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 116, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (123.47, 67.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (182.37, 88.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.952 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13288, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (173.05, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (185.37, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.349 CPU sec, 7.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13101, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 121, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (153.83, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.98, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.604 CPU sec, 5.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13230, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 108, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (94.67, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.83, 91.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.454 CPU sec, 6.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13372, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 109, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (134.62, 71.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.55, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.889 CPU sec, 8.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13198, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 153, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (186.56, 131.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.99, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.911 CPU sec, 6.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13243, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 133, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (142.88, 79.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (188.50, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.722 CPU sec, 6.543 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12991, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (126.24, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.77, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.122 CPU sec, 8.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13242, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (138.29, 82.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.81, 91.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.012 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13208, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 105, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (121.89, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.89, 90.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.149 CPU sec, 6.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13131, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 89, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (104.70, 46.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.86, 92.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.270 CPU sec, 7.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13119, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (141.17, 68.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (184.81, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.107 CPU sec, 6.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13348, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 174, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (180.00, 93.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.30, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.692 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13185, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 159, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (171.50, 76.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.57, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.166 CPU sec, 7.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13461, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 117, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (144.68, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (182.62, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.808 CPU sec, 5.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13220, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 104, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (111.67, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.88, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.081 CPU sec, 6.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13292, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (101.10, 47.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.82, 93.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.357 CPU sec, 5.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13302, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (120.21, 51.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (188.33, 93.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.731 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13305, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (151.48, 106.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (190.73, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.008 CPU sec, 6.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13345, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (131.47, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.00, 94.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.133 CPU sec, 6.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13353, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 180, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (151.00, 57.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.95, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.996 CPU sec, 6.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13295, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (132.00, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.32, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.584 CPU sec, 7.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13246, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 155, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (163.44, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.47, 95.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.350 CPU sec, 5.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13355, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 106, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (117.38, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.23, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.911 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13453, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 97, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (107.80, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.37, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.049 CPU sec, 6.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13236, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (155.24, 94.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (188.19, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.330 CPU sec, 7.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13166, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 130, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (130.17, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.39, 92.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.558 CPU sec, 6.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13347, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 111, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (130.19, 101.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.62, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.302 CPU sec, 7.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13100, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 111, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (111.06, 60.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (184.27, 90.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.588 CPU sec, 6.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13172, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 139, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.28, 71.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (182.99, 89.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.475 CPU sec, 5.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13159, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (146.35, 84.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.58, 89.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.183 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12956, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 188, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (207.60, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.01, 86.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.719 CPU sec, 8.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12765, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 117, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (134.40, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (179.68, 86.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.993 CPU sec, 6.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13290, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.06, 60.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (192.72, 98.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.750 CPU sec, 7.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13276, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (123.44, 52.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (191.31, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.624 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13171, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 223, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (218.57, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 166, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (191.65, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.393 CPU sec, 6.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13136, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 141, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (135.05, 83.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (191.04, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.566 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13367, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 127, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (127.75, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (194.74, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.142 CPU sec, 5.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13197, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 157, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (144.33, 83.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (190.71, 95.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.646 CPU sec, 7.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13300, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 79, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (95.12, 38.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.41, 95.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.210 CPU sec, 6.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13265, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 158, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (158.60, 112.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (192.50, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.275 CPU sec, 7.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13245, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (204.38, 169.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.46, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.675 CPU sec, 7.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13327, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 136, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (127.94, 65.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (189.78, 95.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.208 CPU sec, 6.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13375, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 127, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (151.36, 84.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.50, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.142 CPU sec, 8.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13309, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 162, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (194.70, 103.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (185.06, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.106 CPU sec, 6.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13138, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (143.69, 76.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (184.47, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.092 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13054, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 174, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (198.42, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (182.05, 88.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.964 CPU sec, 5.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13135, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 210, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (213.59, 130.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (180.88, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.050 CPU sec, 6.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12987, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 158, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (153.33, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (180.91, 86.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.420 CPU sec, 6.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12921, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 162, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (182.29, 107.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (188.01, 93.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.973 CPU sec, 6.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13269, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 79, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (87.23, 38.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (192.73, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.059 CPU sec, 6.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13073, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 166, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (154.52, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (191.16, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.092 CPU sec, 6.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13160, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 129, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (163.85, 81.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (191.09, 96.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.460 CPU sec, 6.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13124, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 110, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (124.94, 58.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (191.08, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.351 CPU sec, 8.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13205, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 112, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (144.23, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (189.47, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.098 CPU sec, 5.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13229, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (114.37, 61.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (188.64, 93.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.773 CPU sec, 6.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13053, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (144.10, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (188.93, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.588 CPU sec, 7.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13317, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 222, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (181.82, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (190.09, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.067 CPU sec, 6.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13141, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 96, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (123.38, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.86, 94.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.232 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13332, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (129.95, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.11, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.386 CPU sec, 5.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13310, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 209, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (189.65, 106.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (183.72, 90.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.558 CPU sec, 6.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13181, 9, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 196, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (161.42, 108.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.53, 91.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.260 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13361, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 95, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (95.08, 29.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (181.47, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.484 CPU sec, 6.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13248, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 117, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (141.09, 71.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (181.13, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.384 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12959, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 130, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (150.13, 67.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.33, 87.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.296 CPU sec, 5.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12822, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 131, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (142.75, 61.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (181.62, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.698 CPU sec, 6.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13194, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (121.64, 61.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (189.28, 94.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.769 CPU sec, 6.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13289, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 173, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (182.85, 110.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.12, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.851 CPU sec, 6.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13056, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 105, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (118.53, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.11, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.632 CPU sec, 7.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13069, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 155, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (199.55, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.45, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.998 CPU sec, 6.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13271, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 137, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (179.18, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (189.80, 95.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.441 CPU sec, 5.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13280, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 139, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (172.00, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.96, 94.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.627 CPU sec, 6.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12980, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 123, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (155.79, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.95, 93.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.992 CPU sec, 7.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13265, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (106.67, 46.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.13, 92.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.842 CPU sec, 7.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13255, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 125, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (104.60, 45.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.59, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.518 CPU sec, 6.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13085, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 146, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (147.92, 92.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.21, 92.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.972 CPU sec, 6.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13144, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 125, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (118.76, 57.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.49, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.392 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13180, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 109, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (106.43, 36.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.61, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.770 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13285, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (121.47, 65.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (182.67, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.063 CPU sec, 5.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13229, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 145, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (132.23, 47.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.11, 89.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.051 CPU sec, 6.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13245, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 140, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (150.56, 74.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (180.87, 87.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.734 CPU sec, 7.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12977, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (122.45, 46.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (179.05, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.183 CPU sec, 6.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13027, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 168, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (160.47, 62.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (181.11, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.298 CPU sec, 5.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13186, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 100, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (111.57, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (188.41, 94.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.083 CPU sec, 7.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13312, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 145, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (146.29, 53.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (190.08, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.218 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13241, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 235, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (142, 342) [M::mem_pestat] mean and std.dev: (240.62, 35.35) [M::mem_pestat] low and high boundaries for proper pairs: (99, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.69, 94.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.890 CPU sec, 7.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13288, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 113, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 234) [M::mem_pestat] mean and std.dev: (116.30, 37.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (191.06, 95.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.086 CPU sec, 6.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13269, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (135.40, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (190.71, 95.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.635 CPU sec, 7.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13139, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (145.50, 68.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.30, 95.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.989 CPU sec, 6.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13220, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (112.53, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (190.47, 95.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.173 CPU sec, 7.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13289, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 96, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (132.92, 102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (188.61, 93.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.363 CPU sec, 8.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13272, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 159, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (153.82, 66.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.65, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.743 CPU sec, 7.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13002, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 113, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (134.75, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.10, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.929 CPU sec, 6.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13269, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 181, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (150.70, 38.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.15, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.877 CPU sec, 6.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13259, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 149, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (157.45, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (182.27, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.103 CPU sec, 5.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13295, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 126, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (136.09, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.34, 90.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.547 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13235, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 160, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (170.50, 77.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (181.73, 89.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.880 CPU sec, 6.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13075, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 161, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (165.25, 70.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (179.20, 85.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.433 CPU sec, 5.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12755, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 120, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (142.12, 85.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (177.12, 84.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.546 CPU sec, 6.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13081, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 149, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (168.33, 109.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (189.90, 95.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.687 CPU sec, 7.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13363, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 120, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (127.40, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (191.68, 96.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.394 CPU sec, 8.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13314, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 161, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (135.24, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (190.19, 96.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.028 CPU sec, 5.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13138, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 128, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (147.19, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.93, 93.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.142 CPU sec, 6.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13249, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (161.80, 66.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.96, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.560 CPU sec, 7.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13223, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 166, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (165.95, 110.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.81, 94.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.918 CPU sec, 6.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13057, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 85, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (152.19, 129.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (190.47, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.258 CPU sec, 7.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13264, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (121.73, 69.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.74, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.675 CPU sec, 7.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13041, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 160, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (178.71, 134.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.97, 93.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.857 CPU sec, 7.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13253, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (124.76, 58.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.78, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.393 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13197, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 148, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (144.73, 63.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (185.43, 92.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.978 CPU sec, 7.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13184, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 143, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (156.74, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.81, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.692 CPU sec, 6.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13360, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 116, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (146.85, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (183.07, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.546 CPU sec, 6.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13230, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 119, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (136.22, 74.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (181.92, 88.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.519 CPU sec, 6.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13231, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 133, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (153.24, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (179.20, 85.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.507 CPU sec, 7.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13084, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 123, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (118.30, 63.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (179.04, 85.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.261 CPU sec, 7.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12902, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 151, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (141.10, 49.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (181.01, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.250 CPU sec, 5.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13324, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 139, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 252) [M::mem_pestat] mean and std.dev: (134.10, 29.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.89, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.262 CPU sec, 6.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13341, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (129.41, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.33, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.623 CPU sec, 7.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13356, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 214, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (183.86, 112.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (182.00, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.594 CPU sec, 7.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13277, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (137.89, 65.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.03, 91.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.163 CPU sec, 6.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13163, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 143, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (174.36, 116.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (183.05, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.275 CPU sec, 6.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13288, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 120, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (135.00, 88.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (188.26, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.606 CPU sec, 7.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13197, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (113.05, 52.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.53, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.511 CPU sec, 7.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13318, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 180, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (196.55, 100.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.23, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.889 CPU sec, 6.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13273, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (96.38, 49.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.69, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.873 CPU sec, 7.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13284, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 133, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (161.19, 105.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.92, 92.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.097 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12897, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 214, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (191.94, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.03, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.474 CPU sec, 6.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13054, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (114.82, 48.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.95, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.406 CPU sec, 7.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13267, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 101, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (102.00, 41.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.16, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.058 CPU sec, 6.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13234, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 155, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (137.75, 67.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (188.00, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.839 CPU sec, 6.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13378, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 124, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (158.62, 96.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.81, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.704 CPU sec, 7.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13287, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (139.43, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (188.31, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.635 CPU sec, 6.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13490, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 146, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (145.95, 83.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.33, 92.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.297 CPU sec, 6.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13313, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 98, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (136.55, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (183.60, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.119 CPU sec, 6.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13385, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 185, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (182.29, 93.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.47, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.061 CPU sec, 6.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13260, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 85, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (113.38, 63.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.12, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.859 CPU sec, 5.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13274, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 131, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (134.21, 71.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.78, 91.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.253 CPU sec, 6.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13294, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 153, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (181.52, 111.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (187.08, 92.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.518 CPU sec, 7.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13370, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 113, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (132.05, 64.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (187.78, 94.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.472 CPU sec, 7.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13546, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (200.42, 175.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.02, 92.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.568 CPU sec, 7.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13382, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 148, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (157.13, 78.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.12, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.319 CPU sec, 6.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13321, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (180.25, 82.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.24, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.923 CPU sec, 7.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13084, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 131, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (151.14, 88.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.99, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.085 CPU sec, 6.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13062, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 151, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (162.80, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.72, 91.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.289 CPU sec, 5.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13253, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (147.60, 89.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.99, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.888 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13156, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 176, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (195.53, 139.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.18, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.011 CPU sec, 6.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13186, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 191, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (192.64, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.44, 94.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.020 CPU sec, 5.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13256, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (154.54, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.12, 94.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.996 CPU sec, 5.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13561, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (152.06, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.96, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.044 CPU sec, 6.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13395, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 137, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (139.32, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.28, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.420 CPU sec, 5.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13408, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 139, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (145.44, 63.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (184.11, 90.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.654 CPU sec, 7.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13059, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 125, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (118.63, 64.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.53, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.637 CPU sec, 6.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13144, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (112.46, 55.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (182.37, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.145 CPU sec, 5.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13256, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (100.18, 38.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.40, 94.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.938 CPU sec, 7.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13139, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 164, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (141.10, 69.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.40, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.551 CPU sec, 7.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13226, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 169, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (182.81, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.81, 94.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.008 CPU sec, 5.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13291, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 161, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (149.64, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (187.51, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.692 CPU sec, 6.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13282, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 116, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (111.62, 43.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.15, 92.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.798 CPU sec, 5.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13299, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 148, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (149.41, 65.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (184.80, 90.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.047 CPU sec, 7.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13068, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 99, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (169.06, 133.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.26, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.555 CPU sec, 7.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13372, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (126.05, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.06, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.447 CPU sec, 5.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13277, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 169, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (167.10, 103.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.74, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.901 CPU sec, 7.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13257, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.44, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.168 CPU sec, 6.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13307, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 150, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (152.84, 78.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.73, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.225 CPU sec, 7.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13360, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 123, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (181.95, 105.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.74, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.258 CPU sec, 6.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13382, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 195, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (198.62, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.77, 90.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.985 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13376, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (116.47, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (182.06, 88.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.407 CPU sec, 5.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13272, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 106, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (111.70, 50.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.74, 89.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.927 CPU sec, 5.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13348, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (143.00, 101.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.74, 90.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.628 CPU sec, 5.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13426, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 134, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (149.87, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.20, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.220 CPU sec, 8.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13246, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 113, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (125.65, 51.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.05, 93.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.787 CPU sec, 7.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13162, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 128, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (163.40, 86.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.35, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.023 CPU sec, 6.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13198, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 138, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (164.00, 80.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.18, 93.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.857 CPU sec, 7.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13256, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (130.50, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.21, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.389 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13197, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.02, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 142.056 CPU sec, 6.033 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12967, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (181.96, 89.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.997 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13166, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 108, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (135.28, 91.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.36, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.298 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13140, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 148, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (139.62, 59.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.73, 90.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.590 CPU sec, 5.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13323, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 108, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (102.05, 52.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.65, 91.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.306 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13476, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 114, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (117.82, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (185.67, 93.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.477 CPU sec, 5.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13283, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 138, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (141.90, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.85, 91.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.212 CPU sec, 6.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13286, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (185.17, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.363 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13368, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 115, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (138.36, 79.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.34, 90.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.366 CPU sec, 6.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13384, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 124, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (132.82, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (181.87, 87.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.886 CPU sec, 6.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13371, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 117, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (124.91, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.47, 91.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.702 CPU sec, 5.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13432, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (140.50, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.07, 93.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.782 CPU sec, 6.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13157, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 97, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (131.60, 79.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.52, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.335 CPU sec, 5.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13119, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 177, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (169.60, 105.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.21, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.056 CPU sec, 6.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13367, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 150, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (146.58, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (183.19, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.711 CPU sec, 6.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13425, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 152, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (146.22, 58.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.83, 91.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.625 CPU sec, 7.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13359, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 156, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (162.33, 84.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.24, 92.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.711 CPU sec, 6.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12904, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 150, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (186.38, 140.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (184.62, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.533 CPU sec, 5.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13133, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 146, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (145.74, 59.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (183.76, 88.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.292 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13221, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 89, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (113.54, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.31, 89.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.041 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13130, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 144, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (158.69, 84.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.04, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.893 CPU sec, 5.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13371, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (184.38, 90.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.158 CPU sec, 6.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13331, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.53, 92.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.930 CPU sec, 5.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13409, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 143, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (161.36, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (183.27, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.892 CPU sec, 7.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13373, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 139, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (188.53, 118.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (182.92, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.674 CPU sec, 6.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13388, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 133, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (159.00, 105.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.53, 88.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.331 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13198, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 143, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (145.20, 61.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.29, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.164 CPU sec, 6.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13330, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 127, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (157.50, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.31, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.362 CPU sec, 5.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13431, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 179, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 386) [M::mem_pestat] mean and std.dev: (198.69, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (186.50, 92.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.702 CPU sec, 5.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13190, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 108, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (152.95, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (185.26, 92.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.438 CPU sec, 6.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13331, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 173, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (189.46, 142.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (185.17, 90.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.574 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13282, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 160, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (170.69, 66.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (185.10, 90.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.516 CPU sec, 6.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13301, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 124, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (138.38, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.97, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.272 CPU sec, 6.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13111, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 160, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (183.40, 89.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.63, 89.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.527 CPU sec, 5.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13287, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (129.00, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (183.51, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.649 CPU sec, 5.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13223, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 159, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (206.78, 139.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (184.03, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.966 CPU sec, 6.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13376, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 125, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (115.47, 50.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.99, 92.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.682 CPU sec, 7.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13324, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 142, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (146.69, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.41, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.966 CPU sec, 6.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13309, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 89, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (156.20, 141.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.75, 91.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.127 CPU sec, 6.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13268, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (183.98, 89.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.133 CPU sec, 6.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13427, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 121, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (138.72, 90.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.16, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.006 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13100, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 102, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (91.47, 46.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (180.23, 86.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.815 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13002, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 195, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (183.25, 71.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (179.69, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.141 CPU sec, 5.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12848, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 151, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (150.44, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (181.11, 86.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.275 CPU sec, 6.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13295, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 142, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (140.06, 60.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (188.22, 94.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.835 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13345, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 166, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (138.38, 79.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.97, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.434 CPU sec, 6.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13283, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 169, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (159.68, 59.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.99, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.452 CPU sec, 5.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13121, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 126, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (116.50, 43.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.29, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.660 CPU sec, 5.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13347, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 122, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (144.94, 82.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.83, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.829 CPU sec, 5.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13318, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 107, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 181) [M::mem_pestat] mean and std.dev: (105.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (13, 209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.99, 93.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.825 CPU sec, 6.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13284, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 108, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.83, 51.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.68, 94.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.423 CPU sec, 5.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13081, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 106, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (121.71, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.25, 93.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.840 CPU sec, 5.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13168, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 136, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (135.04, 61.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (189.06, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.873 CPU sec, 5.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13096, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 103, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (109.44, 67.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.79, 94.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.319 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13161, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 117, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (114.10, 56.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.70, 92.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.857 CPU sec, 5.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13166, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (84.14, 37.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.15, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.730 CPU sec, 7.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13136, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 96, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (124.82, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (182.76, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.269 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13150, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (130.14, 64.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.17, 86.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.051 CPU sec, 6.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12805, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 121, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 205) [M::mem_pestat] mean and std.dev: (117.44, 36.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.47, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.626 CPU sec, 7.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12804, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 157, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (157.76, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (181.87, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.371 CPU sec, 7.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13326, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 226, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (227.28, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (189.49, 94.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.611 CPU sec, 6.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13271, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 126, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (143.39, 83.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.63, 94.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.246 CPU sec, 6.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13370, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 138, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (132.39, 66.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.45, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.983 CPU sec, 6.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13506, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.94, 92.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.663 CPU sec, 5.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13221, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 129, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (165.43, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.88, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.707 CPU sec, 7.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13341, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 142, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (140.67, 78.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (185.64, 91.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.919 CPU sec, 6.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13306, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 137, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (129.29, 60.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (188.63, 94.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.756 CPU sec, 7.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13350, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 111, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (141.33, 90.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (187.86, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.361 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13241, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 117, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (176.25, 143.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (190.68, 95.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.555 CPU sec, 7.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13228, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 182, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (185.85, 111.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (187.94, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.407 CPU sec, 7.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13359, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (121.47, 48.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (189.25, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.467 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13324, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 122, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (153.65, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (182.99, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.360 CPU sec, 6.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13292, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 128, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (144.56, 92.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.03, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.951 CPU sec, 6.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13206, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (110.62, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (180.47, 87.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.062 CPU sec, 7.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13204, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 194, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (172.08, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (181.88, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.539 CPU sec, 6.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12907, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (143.68, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.46, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.248 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13283, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 115, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (156.47, 99.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.61, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.883 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13183, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 174, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (163.85, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.10, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.708 CPU sec, 6.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13185, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 118, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (143.75, 85.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.73, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.150 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13363, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 178, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (157.96, 85.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (189.59, 95.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.456 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13178, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 157, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (150.04, 97.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.74, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.294 CPU sec, 6.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13353, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 129, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (163.47, 91.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.63, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.122 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13336, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 96, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (101.13, 55.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.73, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.157 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13352, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 108, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (127.68, 71.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.77, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.135 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13299, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (148.35, 63.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (190.58, 95.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.597 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13422, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 128, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (158.15, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.56, 93.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.268 CPU sec, 7.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13432, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 178, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (215.05, 108.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (190.07, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.944 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13149, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 109, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (130.71, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.69, 89.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.259 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13189, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 117, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (134.58, 75.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.57, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.835 CPU sec, 7.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13217, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 139, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (137.90, 61.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (179.61, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.696 CPU sec, 6.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12983, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 134, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (136.43, 55.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (177.80, 85.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.291 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12552, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 103, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (111.15, 64.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (176.70, 83.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.663 CPU sec, 7.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13214, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (171.52, 95.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (185.90, 92.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.599 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13090, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 127, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (133.00, 66.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (185.91, 92.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.255 CPU sec, 6.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13094, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (134.20, 77.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (184.46, 90.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.759 CPU sec, 6.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13140, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 155, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (175.78, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.41, 91.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.952 CPU sec, 6.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13225, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 116, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (121.44, 53.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.40, 90.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.427 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13207, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (119.38, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (183.64, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.146 CPU sec, 6.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13225, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 98, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (100.06, 43.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (186.37, 92.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.892 CPU sec, 7.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13220, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 100, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (100.93, 57.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (188.99, 94.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.687 CPU sec, 6.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13188, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (134.47, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (187.52, 93.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.675 CPU sec, 5.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13286, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 120, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (131.38, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.85, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.202 CPU sec, 7.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13390, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 103, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (115.67, 61.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.80, 93.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.424 CPU sec, 6.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13219, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 121, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (123.58, 46.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (183.51, 90.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.755 CPU sec, 6.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13309, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 124, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (130.43, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (181.02, 87.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.827 CPU sec, 5.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13311, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 155, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (167.18, 106.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (178.77, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.861 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13051, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (143.95, 80.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (177.07, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.776 CPU sec, 6.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12782, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 227, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (216.69, 94.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 156, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (176.45, 81.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.414 CPU sec, 7.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13066, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 122, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (141.53, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.70, 92.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.373 CPU sec, 6.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13329, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (138.04, 84.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (187.32, 93.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.771 CPU sec, 6.089 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13144, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 147, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 255) [M::mem_pestat] mean and std.dev: (129.54, 53.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.21, 91.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.419 CPU sec, 7.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13382, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 92, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (119.73, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (185.08, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.796 CPU sec, 5.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13278, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 113, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (146.84, 78.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (186.41, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.000 CPU sec, 7.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13475, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 153, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (147.27, 92.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (183.92, 90.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.370 CPU sec, 6.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13229, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 132, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 264) [M::mem_pestat] mean and std.dev: (131.82, 43.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (187.47, 92.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.080 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13187, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 87, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (126.95, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (186.98, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.201 CPU sec, 7.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13248, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 99, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (100.22, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.22, 93.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.144 CPU sec, 6.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13281, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 137, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (151.75, 89.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (186.42, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.006 CPU sec, 6.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13273, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (187.01, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.882 CPU sec, 7.785 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13302, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (139.48, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.68, 91.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.420 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13247, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (155.25, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (182.26, 88.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.442 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13053, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 156, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (147.84, 65.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (180.60, 87.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.226 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13332, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 179, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (172.58, 92.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (180.21, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.732 CPU sec, 5.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12882, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (131.79, 67.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (178.93, 85.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.786 CPU sec, 7.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13183, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 130, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (153.85, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (184.48, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.702 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13309, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 143, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (126.22, 56.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.52, 93.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.740 CPU sec, 6.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13260, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 156, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (167.00, 103.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (185.50, 91.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.258 CPU sec, 5.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13486, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 125, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (131.21, 77.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (186.09, 92.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.933 CPU sec, 6.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13408, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 161, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (168.70, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (184.70, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.484 CPU sec, 7.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13491, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 125, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (127.92, 78.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.68, 90.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.825 CPU sec, 6.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13410, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (131.39, 72.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (188.52, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.711 CPU sec, 4.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13402, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 89, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (119.71, 74.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (184.90, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.807 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13444, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 137, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (162.13, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (185.87, 92.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.500 CPU sec, 5.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13218, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 171, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (239.62, 258.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (187.61, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.380 CPU sec, 6.978 real sec [M::process] read 3066 sequences (462966 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13330, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (146.04, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (186.83, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.509 CPU sec, 6.406 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 630, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (123, 165, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (189.16, 91.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 3066 reads in 8.417 CPU sec, 0.885 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21180\tSM:UG10S2P486\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21180_HLKNYBGXX_UG10S2P486_R1.fastq.gz 5347_7332_21180_HLKNYBGXX_UG10S2P486_R2.fastq.gz [main] Real time: 2537.843 sec; CPU: 63463.637 sec Finished alignement of UG10S2P486 at Wed Apr 3 16:25:32 2019 Sorting BAM file started at Wed Apr 3 16:25:32 2019 samtools sort -@ 30 -m 4G UG10S2P486_unsorted.bam -o UG10S2P486.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 16:25:32 2019 rm UG10S2P486_unsorted.bam rm 5347_7332_21180_HLKNYBGXX_UG10S2P486_R1.fastq.gz rm 5347_7332_21180_HLKNYBGXX_UG10S2P486_R2.fastq.gz samtools index UG10S2P486.bam Finished BAM file generation of the sample - UG10S2P486 Processing fastq files for the sample - UG10S2P499 Reading 5347_7332_21181_HLKNYBGXX_UG10S2P499_R1.fastq.gz 5347_7332_21181_HLKNYBGXX_UG10S2P499_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21181\tSM:UG10S2P499\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21181_HLKNYBGXX_UG10S2P499_R1.fastq.gz 5347_7332_21181_HLKNYBGXX_UG10S2P499_R2.fastq.gz | samtools view -Sb1 - > UG10S2P499_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12134, 0, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.37, 43.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.784 CPU sec, 7.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12144, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.34, 43.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.093 CPU sec, 6.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12223, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (136.98, 42.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.274 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12239, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 106, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (93.80, 38.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.76, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.716 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12351, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.20, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.723 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12295, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.55, 43.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.003 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12286, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (91.38, 31.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.02, 43.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.411 CPU sec, 6.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12145, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 63, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (100.82, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.93, 43.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.618 CPU sec, 7.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12369, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.54, 43.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.314 CPU sec, 6.856 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12246, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.41, 42.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 180.922 CPU sec, 7.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12258, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.27, 43.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.216 CPU sec, 5.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12280, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (155.60, 105.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.00, 43.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.282 CPU sec, 6.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12239, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.15, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 178.153 CPU sec, 7.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12221, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.29, 43.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.705 CPU sec, 6.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12279, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.93, 43.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.032 CPU sec, 5.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12456, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 75, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (75.38, 41.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.81, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.327 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12518, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 109, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (110.60, 57.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.48, 44.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.136 CPU sec, 5.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12398, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 91, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (105.92, 74.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (138.40, 44.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.882 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12397, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.11, 43.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.567 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12338, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 96, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (97.25, 37.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.64, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.944 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12301, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 118, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (125.69, 63.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.22, 43.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.821 CPU sec, 5.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12260, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.31, 42.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.576 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12142, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.08, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 193.724 CPU sec, 8.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12238, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (111.00, 33.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 42.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.052 CPU sec, 7.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12274, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.95, 43.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.283 CPU sec, 6.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12229, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.96, 43.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.553 CPU sec, 6.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12304, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.02, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.057 CPU sec, 6.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12411, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.81, 42.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.261 CPU sec, 6.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12345, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 73, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (75.71, 50.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.02, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.794 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12246, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.67, 42.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.168 CPU sec, 7.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12326, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 59, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (70.17, 35.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.56, 43.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.804 CPU sec, 6.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12430, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.20, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.093 CPU sec, 6.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12324, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 76, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (113.85, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.12, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.875 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12129, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.99, 42.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.789 CPU sec, 6.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12242, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 83, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (98.15, 60.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.67, 44.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.341 CPU sec, 7.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12312, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.57, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.713 CPU sec, 6.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12187, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 75, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (91.91, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.42, 42.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.307 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12516, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.93, 42.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.650 CPU sec, 6.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12500, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.76, 42.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.141 CPU sec, 5.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12443, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 98, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (114.80, 91.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.90, 44.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.982 CPU sec, 5.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12356, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.88, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.308 CPU sec, 6.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12574, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.65, 44.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.052 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12394, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.43, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.671 CPU sec, 5.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12414, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 82, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (87.64, 45.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.27, 42.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.889 CPU sec, 7.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12446, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.24, 43.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.559 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12076, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 96, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (99.50, 44.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.18, 43.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.818 CPU sec, 7.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12155, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.19, 43.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.635 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12193, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.44, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 177.488 CPU sec, 6.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12387, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 87, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (89.18, 35.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.59, 42.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.612 CPU sec, 6.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12285, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 102, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 214) [M::mem_pestat] mean and std.dev: (99.70, 26.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.93, 42.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.795 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12233, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.51, 43.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.436 CPU sec, 6.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12210, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.70, 43.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.352 CPU sec, 7.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12221, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 77, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (75.00, 39.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (136.46, 43.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.125 CPU sec, 6.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12255, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.50, 43.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.568 CPU sec, 7.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12351, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (137.40, 44.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.231 CPU sec, 7.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12188, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.93, 42.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 177.497 CPU sec, 7.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12172, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 106, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (103.69, 57.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.36, 42.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.655 CPU sec, 6.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12319, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.18, 43.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.199 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12299, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.30, 43.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.880 CPU sec, 6.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12180, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.32, 44.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.155 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12424, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.27, 43.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.285 CPU sec, 5.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12336, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 82, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (73.36, 40.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.13, 43.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.567 CPU sec, 6.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12524, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 100, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (98.22, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.72, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.640 CPU sec, 6.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12290, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 155, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (144.40, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.59, 42.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.512 CPU sec, 5.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12415, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 50, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (80.70, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.87, 42.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.640 CPU sec, 5.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12349, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.17, 43.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.561 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12125, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 106, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (103.21, 49.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.04, 42.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.610 CPU sec, 4.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12097, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.07, 42.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.940 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12300, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 120, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (129.20, 98.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.81, 42.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.459 CPU sec, 5.217 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12133, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.10, 42.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.603 CPU sec, 5.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12312, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.35, 42.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.504 CPU sec, 6.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12156, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.78, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.024 CPU sec, 6.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12348, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.19, 43.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.784 CPU sec, 6.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12299, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.98, 43.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.103 CPU sec, 6.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12144, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.80, 43.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 184.766 CPU sec, 7.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12030, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 97, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (132.05, 87.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.60, 43.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.617 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11997, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 97, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (87.36, 33.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (135.33, 41.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.934 CPU sec, 6.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12209, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 63, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (103.73, 77.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.26, 43.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.313 CPU sec, 7.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12186, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.69, 42.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 180.325 CPU sec, 7.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12058, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 95, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (107.62, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.81, 42.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.733 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12102, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 95, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (84.80, 36.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.04, 43.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.292 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12125, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.49, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.742 CPU sec, 5.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12264, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 107, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (108.00, 48.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.63, 42.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.456 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12263, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 86, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (86.65, 38.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.45, 42.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.504 CPU sec, 5.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12405, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 74, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 142) [M::mem_pestat] mean and std.dev: (68.30, 27.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.63, 43.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.110 CPU sec, 5.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12426, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 115, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (125.30, 88.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.92, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.880 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12323, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 66, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (81.64, 47.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.58, 42.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.980 CPU sec, 4.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12249, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 61, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (87.00, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 43.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.520 CPU sec, 4.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12363, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (189.00, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.29, 42.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.091 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12147, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 104, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (98.70, 40.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.08, 42.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.667 CPU sec, 6.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12243, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.649 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12178, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.06, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.826 CPU sec, 6.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12104, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.14, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.699 CPU sec, 6.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12324, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.46, 42.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.924 CPU sec, 5.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12369, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 142, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 272) [M::mem_pestat] mean and std.dev: (143.36, 43.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.55, 42.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.606 CPU sec, 6.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12480, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.50, 42.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.139 CPU sec, 6.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12239, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 80, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (75.18, 40.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.99, 42.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.877 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12258, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 76, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (90.00, 47.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.47, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.712 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12236, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.14, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.306 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12272, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 124, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (147.33, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.19, 43.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.319 CPU sec, 6.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12202, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.61, 42.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.326 CPU sec, 6.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12332, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 118, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (121.00, 83.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.95, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.607 CPU sec, 7.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12244, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.80, 44.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.751 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12173, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (137.81, 44.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.815 CPU sec, 6.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12391, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.86, 42.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.222 CPU sec, 7.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12467, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.32, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.217 CPU sec, 6.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12492, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.30, 42.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.627 CPU sec, 5.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12540, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.12, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.857 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12394, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 81, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (93.15, 39.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.77, 42.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.179 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12351, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.82, 42.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.468 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12389, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (136.54, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.56, 42.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.111 CPU sec, 5.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12237, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.52, 42.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.251 CPU sec, 6.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12153, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.13, 42.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.615 CPU sec, 5.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12295, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.76, 42.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.346 CPU sec, 7.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12045, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 94, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (83.89, 28.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.05, 42.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.544 CPU sec, 6.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12211, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.67, 42.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.116 CPU sec, 8.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12350, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (136.80, 43.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.069 CPU sec, 6.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12159, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.44, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.386 CPU sec, 6.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12335, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 59, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (68.64, 36.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.65, 43.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.567 CPU sec, 6.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12193, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.65, 43.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.513 CPU sec, 6.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 11961, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.01, 43.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.242 CPU sec, 6.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12156, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 92, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (140.46, 89.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.69, 41.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.735 CPU sec, 6.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12152, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.29, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 184.919 CPU sec, 6.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12027, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 77, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (121.80, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.73, 43.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.165 CPU sec, 6.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12076, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 106, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (97.00, 45.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.62, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.283 CPU sec, 5.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12105, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 79, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (88.93, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.78, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.292 CPU sec, 7.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12169, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 99, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (81.11, 31.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.12, 42.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.771 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12279, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.45, 43.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.863 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12141, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 90, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (90.64, 36.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.96, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.937 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12300, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.92, 43.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.771 CPU sec, 7.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12146, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.28, 42.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.735 CPU sec, 6.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12386, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 87, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (98.50, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.71, 42.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.915 CPU sec, 6.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12400, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.01, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.485 CPU sec, 6.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12323, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.17, 43.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 183.694 CPU sec, 19.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12257, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.28, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.020 CPU sec, 6.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12247, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (164.58, 161.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.74, 43.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.494 CPU sec, 5.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12312, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 85, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (96.42, 47.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 135, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.62, 43.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.768 CPU sec, 6.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12286, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 77, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (93.60, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.01, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.362 CPU sec, 5.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12317, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 79, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (96.83, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.52, 43.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.750 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12331, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.67, 42.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.589 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12241, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.94, 42.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.786 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12184, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.51, 43.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.737 CPU sec, 7.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12105, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 129, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (149.38, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.48, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.686 CPU sec, 6.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12085, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.04, 42.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.462 CPU sec, 7.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12253, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.53, 43.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.542 CPU sec, 6.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12278, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.32, 43.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.010 CPU sec, 6.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12289, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 111, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 197) [M::mem_pestat] mean and std.dev: (104.18, 33.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.66, 43.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.411 CPU sec, 5.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12442, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.61, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.382 CPU sec, 6.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12268, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.87, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.653 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12360, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 66, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (72.82, 40.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.60, 42.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.986 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12264, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 83, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (102.50, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (135.84, 41.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.776 CPU sec, 6.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12278, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 95, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (96.00, 47.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.69, 41.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.380 CPU sec, 6.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12454, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.68, 43.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.758 CPU sec, 5.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12160, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 72, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (79.67, 50.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.34, 42.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.507 CPU sec, 5.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12121, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.38, 42.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.350 CPU sec, 5.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11886, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.94, 42.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.719 CPU sec, 6.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12213, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (138.25, 45.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.733 CPU sec, 6.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12167, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 70, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (62.44, 18.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.12, 43.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.614 CPU sec, 7.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12132, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (138.37, 44.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.031 CPU sec, 5.626 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12235, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.93, 43.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.798 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12118, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.75, 43.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.075 CPU sec, 6.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12198, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 103, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (88.00, 32.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.18, 43.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.224 CPU sec, 5.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12244, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.99, 43.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.968 CPU sec, 6.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12298, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.01, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.676 CPU sec, 5.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12315, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 80, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (77.25, 42.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.82, 43.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.964 CPU sec, 8.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12181, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.36, 43.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.424 CPU sec, 6.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12367, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (129.64, 88.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 135, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.98, 44.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.613 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12048, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.24, 42.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.688 CPU sec, 6.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12389, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 66, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (80.08, 43.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.86, 42.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.630 CPU sec, 6.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12278, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 96, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (117.00, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.76, 42.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.258 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12383, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 107, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (104.14, 43.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.35, 42.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.967 CPU sec, 5.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12454, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.91, 43.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.912 CPU sec, 7.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12365, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.90, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.153 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12349, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.27, 43.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 140.003 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12357, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.48, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.603 CPU sec, 6.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12267, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.83, 42.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.315 CPU sec, 6.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12063, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 71, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (90.82, 56.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.43, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.577 CPU sec, 6.647 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11917, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 84, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (97.80, 54.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.39, 43.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.126 CPU sec, 6.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12153, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.21, 43.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.172 CPU sec, 6.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12012, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 84, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (103.00, 65.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.31, 42.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.658 CPU sec, 5.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12242, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 128, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (102.40, 41.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.29, 43.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.885 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12094, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 75, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (102.40, 60.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (138.02, 43.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.864 CPU sec, 5.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12046, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 73, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 129) [M::mem_pestat] mean and std.dev: (72.09, 18.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.57, 44.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.392 CPU sec, 5.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12403, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.19, 42.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.747 CPU sec, 7.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12184, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 147, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (151.71, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.43, 43.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.901 CPU sec, 5.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12129, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.98, 43.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.384 CPU sec, 5.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12075, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (138.09, 44.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.036 CPU sec, 6.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12158, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 89, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (96.00, 58.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (137.94, 44.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.086 CPU sec, 6.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12230, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 78, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (102.00, 48.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.73, 44.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.669 CPU sec, 7.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12210, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.28, 43.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.933 CPU sec, 6.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12344, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 69, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (103.40, 84.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.78, 42.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.464 CPU sec, 7.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12335, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (25, 68, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (72.20, 53.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.79, 43.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.056 CPU sec, 5.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12379, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.42, 42.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.869 CPU sec, 6.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12391, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 89, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (104.83, 85.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.50, 43.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.207 CPU sec, 6.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12209, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.71, 42.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.678 CPU sec, 7.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12318, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 63, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (71.60, 34.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.81, 41.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.966 CPU sec, 6.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12131, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 65, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 131) [M::mem_pestat] mean and std.dev: (61.42, 21.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.71, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.858 CPU sec, 6.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 12326, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.07, 43.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 183.295 CPU sec, 6.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12186, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 96, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 195) [M::mem_pestat] mean and std.dev: (103.64, 29.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.06, 42.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.738 CPU sec, 6.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12004, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 95, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (87.00, 33.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.54, 43.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.098 CPU sec, 7.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12176, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 113, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (99.30, 62.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.16, 42.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.170 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12148, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.86, 43.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.127 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12260, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 59, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (100.80, 82.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.75, 42.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.530 CPU sec, 7.609 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12261, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.37, 42.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.021 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12291, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 84, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (87.82, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.35, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.410 CPU sec, 6.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12050, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 128, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (112.14, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.85, 42.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.482 CPU sec, 6.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12095, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.31, 43.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.814 CPU sec, 7.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12052, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 77, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (90.85, 59.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.60, 42.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.022 CPU sec, 5.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12175, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.46, 43.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.684 CPU sec, 6.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12229, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 69, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 108) [M::mem_pestat] mean and std.dev: (64.43, 8.40) [M::mem_pestat] low and high boundaries for proper pairs: (6, 125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.60, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.802 CPU sec, 6.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12317, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.96, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.638 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12113, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (137.91, 43.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.947 CPU sec, 6.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12096, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 90, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 170) [M::mem_pestat] mean and std.dev: (78.60, 31.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.11, 42.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.682 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12158, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.69, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.071 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12419, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 68, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (64.00, 25.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.22, 43.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.917 CPU sec, 6.071 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12446, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 42.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.271 CPU sec, 5.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12408, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.38, 42.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.431 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12419, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 115, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (111.33, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.23, 42.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.317 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12439, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 117, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (145.00, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.71, 42.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.599 CPU sec, 5.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12245, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.01, 43.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.126 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12246, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.71, 43.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.285 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11941, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 82, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (104.27, 58.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.27, 42.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.544 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12260, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.01, 43.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.223 CPU sec, 6.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12133, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (138.52, 45.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.406 CPU sec, 6.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12215, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 89, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (125.27, 93.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (138.05, 44.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.704 CPU sec, 6.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12211, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 56, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 179) [M::mem_pestat] mean and std.dev: (68.20, 21.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.21, 42.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.891 CPU sec, 6.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12203, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.14, 42.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.540 CPU sec, 6.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12279, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.56, 44.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.453 CPU sec, 5.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12405, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 72, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (74.18, 39.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (137.08, 42.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.983 CPU sec, 5.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12127, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (131.82, 35.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.10, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.385 CPU sec, 6.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12236, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.74, 43.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.830 CPU sec, 6.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12191, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.92, 43.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.257 CPU sec, 6.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12209, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 111, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (139.33, 108.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.04, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.704 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12238, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.32, 43.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.498 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12142, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 100, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (131.10, 98.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.13, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.745 CPU sec, 5.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12361, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (150.10, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.27, 43.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.767 CPU sec, 6.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12417, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 86, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (116.50, 79.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (136.89, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.418 CPU sec, 6.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12372, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.78, 42.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.760 CPU sec, 6.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12207, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (135.80, 41.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.154 CPU sec, 6.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12471, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 96, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (106.54, 33.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.31, 42.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.124 CPU sec, 4.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12243, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.01, 42.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.399 CPU sec, 5.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12072, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.29, 42.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.077 CPU sec, 5.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12069, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (135.72, 41.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.500 CPU sec, 5.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11970, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (135.90, 42.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.129 CPU sec, 5.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12041, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.72, 43.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.749 CPU sec, 8.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12201, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 86, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (101.17, 63.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.50, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.683 CPU sec, 6.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12194, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.59, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.904 CPU sec, 6.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12273, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 114, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (121.70, 59.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.82, 43.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.744 CPU sec, 5.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12101, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.88, 43.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.148 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12164, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.23, 44.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.650 CPU sec, 6.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12306, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.99, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.284 CPU sec, 7.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12172, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.36, 42.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.057 CPU sec, 7.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12086, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 99, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (104.55, 57.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.73, 43.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.172 CPU sec, 7.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12165, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 79, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (85.08, 55.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.12, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.970 CPU sec, 6.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12193, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.05, 43.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.481 CPU sec, 6.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12217, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 97, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (85.36, 55.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.48, 43.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.526 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12177, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.44, 43.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.439 CPU sec, 6.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12153, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.81, 44.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.292 CPU sec, 7.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12210, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.74, 42.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.240 CPU sec, 6.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12160, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.94, 42.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.373 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12381, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 60, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (80.80, 52.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (136.92, 43.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.334 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12434, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 81, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (78.86, 37.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.74, 42.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.276 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12306, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.63, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.845 CPU sec, 5.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12260, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.68, 42.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.488 CPU sec, 5.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12038, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.62, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.734 CPU sec, 5.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 11880, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.91, 42.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.428 CPU sec, 5.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12284, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.74, 42.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.808 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12446, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 107, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (104.00, 57.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.45, 43.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.141 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12421, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 80, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 150) [M::mem_pestat] mean and std.dev: (81.79, 27.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.46, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.799 CPU sec, 5.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12437, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 83, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (130.67, 95.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.21, 42.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.527 CPU sec, 6.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12228, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.11, 42.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.690 CPU sec, 6.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12411, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 69, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (82.60, 60.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.29, 43.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.143 CPU sec, 7.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12467, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.36, 42.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.313 CPU sec, 5.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12277, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 76, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (73.91, 31.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.24, 42.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.985 CPU sec, 6.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12320, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.02, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.156 CPU sec, 7.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12327, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 87, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (93.00, 43.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.64, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.732 CPU sec, 7.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12163, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 122, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (97.64, 43.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.94, 42.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.358 CPU sec, 6.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12352, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 72, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (86.21, 52.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.87, 42.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.839 CPU sec, 7.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12354, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.90, 43.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.203 CPU sec, 6.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12292, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (101.33, 49.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.11, 43.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.479 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12228, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.55, 43.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.357 CPU sec, 6.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12166, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.11, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.495 CPU sec, 6.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12269, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.71, 43.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.649 CPU sec, 6.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12327, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.66, 42.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.646 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12464, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.15, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.540 CPU sec, 6.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12411, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (120.58, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.04, 43.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.971 CPU sec, 7.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12348, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 87, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (85.00, 25.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.76, 42.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.784 CPU sec, 6.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12420, 1, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.66, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.706 CPU sec, 6.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12320, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 110, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (125.15, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.20, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.085 CPU sec, 5.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12379, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 90, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (109.18, 62.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.39, 43.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.547 CPU sec, 6.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12343, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 75, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152) [M::mem_pestat] mean and std.dev: (70.86, 31.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.82, 43.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.465 CPU sec, 5.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12304, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.18, 43.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.245 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12278, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 98, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (90.82, 29.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.68, 43.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.070 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12480, 0, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.40, 43.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.854 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12319, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.82, 43.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 180.278 CPU sec, 6.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12280, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 75, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (77.27, 34.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.95, 44.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.537 CPU sec, 6.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12312, 0, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.44, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 180.715 CPU sec, 6.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12334, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.00, 42.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.865 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12280, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 84, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (158.91, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (135.96, 42.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.752 CPU sec, 5.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12425, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.21, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.248 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12314, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 93, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (94.57, 58.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.10, 43.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.713 CPU sec, 6.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12354, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 111, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (126.25, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.36, 43.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.216 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12166, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.95, 43.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.567 CPU sec, 6.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12174, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.61, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.834 CPU sec, 6.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12324, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 54, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 104) [M::mem_pestat] mean and std.dev: (55.36, 18.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.44, 43.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.021 CPU sec, 5.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12327, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 98, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (128.09, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.33, 43.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.687 CPU sec, 6.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12233, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 92, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (92.64, 41.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.53, 43.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.205 CPU sec, 6.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12249, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 96, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (105.10, 45.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.72, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.459 CPU sec, 6.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12253, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 95, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (79.31, 29.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.22, 43.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.158 CPU sec, 6.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12483, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (122.91, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.42, 43.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.747 CPU sec, 6.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12453, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 81, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (95.46, 57.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.35, 42.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.736 CPU sec, 6.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12484, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 88, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (100.07, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.07, 42.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.398 CPU sec, 5.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12287, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 78, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (80.74, 30.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.09, 42.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.588 CPU sec, 6.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12339, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 75, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 168) [M::mem_pestat] mean and std.dev: (81.00, 33.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (137.07, 42.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.954 CPU sec, 6.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12320, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 81, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (89.00, 50.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.94, 43.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.571 CPU sec, 5.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12488, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.06, 42.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.993 CPU sec, 5.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12232, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 151, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (125.45, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.71, 42.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.842 CPU sec, 5.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12294, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.40, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.924 CPU sec, 6.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12188, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.59, 43.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.760 CPU sec, 6.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12294, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.80, 44.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.734 CPU sec, 6.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12184, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 104, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (140.92, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.69, 43.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.488 CPU sec, 5.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12337, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.43, 43.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.874 CPU sec, 5.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12307, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.54, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.897 CPU sec, 6.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12280, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (136.64, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 177.589 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12126, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 101, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (87.73, 29.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.47, 43.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.432 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12371, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.06, 42.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.134 CPU sec, 5.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12547, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.32, 43.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.773 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12450, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.43, 42.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.491 CPU sec, 6.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 12340, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.78, 43.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.719 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12316, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 204) [M::mem_pestat] mean and std.dev: (117.00, 39.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.57, 42.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.400 CPU sec, 5.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12215, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 94, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (96.00, 57.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.73, 41.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.775 CPU sec, 5.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12182, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 83, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (84.08, 41.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.93, 42.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.945 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12338, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.88, 42.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.403 CPU sec, 5.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12341, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 80, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (90.00, 76.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.23, 43.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.827 CPU sec, 4.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12265, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 106, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 219) [M::mem_pestat] mean and std.dev: (98.30, 21.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.78, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.310 CPU sec, 5.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12300, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.52, 42.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.213 CPU sec, 6.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12303, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.35, 42.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.053 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12243, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 120, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (93.56, 37.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.60, 42.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.562 CPU sec, 7.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12272, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.83, 43.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 188.297 CPU sec, 7.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12153, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.86, 42.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.973 CPU sec, 5.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12058, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 87, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (87.36, 47.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (136.91, 43.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.003 CPU sec, 6.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12198, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 111, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (92.42, 53.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.38, 42.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.529 CPU sec, 6.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12206, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 90, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (96.80, 54.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.41, 42.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.437 CPU sec, 6.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12116, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.45, 43.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.531 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12338, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189) [M::mem_pestat] mean and std.dev: (63.17, 24.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.64, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.614 CPU sec, 7.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12102, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.63, 43.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.563 CPU sec, 6.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12309, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 110, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (75.89, 43.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.10, 42.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.147 CPU sec, 5.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12262, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.03, 42.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 165.977 CPU sec, 8.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12289, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (88.00, 34.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.11, 42.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.944 CPU sec, 6.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12247, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 78, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (122.80, 92.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.27, 43.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.414 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12120, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.10, 43.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 174.082 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12284, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.43, 42.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.804 CPU sec, 6.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12340, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.81, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.946 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12498, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.60, 43.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.543 CPU sec, 6.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12348, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 82, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (77.64, 27.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.96, 42.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.348 CPU sec, 6.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12352, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 86, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (84.58, 47.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.87, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.668 CPU sec, 5.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12438, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.39, 42.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.707 CPU sec, 5.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12450, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 70, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (72.25, 38.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.24, 43.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.607 CPU sec, 5.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12331, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 96, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (110.20, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.81, 42.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.102 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12277, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (135.44, 95.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.17, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.074 CPU sec, 6.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12242, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.78, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.360 CPU sec, 6.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12337, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.45, 42.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.267 CPU sec, 6.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12311, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.17, 43.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.115 CPU sec, 6.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12154, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.80, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.242 CPU sec, 6.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12259, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.90, 43.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.771 CPU sec, 6.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12097, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.05, 43.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.314 CPU sec, 5.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12242, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 95, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (88.64, 37.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.11, 43.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.962 CPU sec, 6.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11959, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 76, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (94.75, 56.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (135.73, 41.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.218 CPU sec, 6.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12306, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.68, 42.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.220 CPU sec, 6.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12151, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.98, 42.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.663 CPU sec, 5.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12273, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 69, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189) [M::mem_pestat] mean and std.dev: (74.45, 26.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (135.99, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.837 CPU sec, 6.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12352, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 105, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (118.90, 62.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.29, 42.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.648 CPU sec, 6.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12305, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 43.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.125 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12326, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.23, 43.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 185.028 CPU sec, 6.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12376, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.55, 43.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.700 CPU sec, 5.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12387, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.13, 42.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.850 CPU sec, 6.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12261, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.45, 43.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.186 CPU sec, 6.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12277, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 73, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (75.10, 28.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.28, 42.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.156 CPU sec, 5.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12254, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (99.70, 41.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.22, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.892 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12474, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 94, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (94.22, 41.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.89, 43.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.314 CPU sec, 6.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12365, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 74, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (71.58, 34.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.33, 43.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.060 CPU sec, 5.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12296, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 71, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (74.36, 43.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.72, 43.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.585 CPU sec, 5.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12270, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 89, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (107.36, 68.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.16, 43.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.381 CPU sec, 6.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12315, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 67, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (100.92, 60.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.51, 42.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.208 CPU sec, 6.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12121, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 122, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (147.90, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.32, 43.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.766 CPU sec, 7.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12288, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 120, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (111.82, 70.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (137.14, 42.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.355 CPU sec, 6.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12305, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 89, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (91.85, 41.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.03, 42.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.378 CPU sec, 6.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12184, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 71, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (75.40, 35.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.44, 43.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.317 CPU sec, 6.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12159, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.42, 42.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.853 CPU sec, 5.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12301, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.09, 43.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 181.304 CPU sec, 6.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12349, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.01, 42.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.609 CPU sec, 5.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12162, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.05, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.269 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12301, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 98, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (133.38, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.61, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.388 CPU sec, 6.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12159, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.95, 42.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 161.420 CPU sec, 5.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12340, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 84, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (85.94, 47.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.06, 43.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.212 CPU sec, 6.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12198, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.20, 42.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.655 CPU sec, 6.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12345, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 80, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (100.70, 54.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.96, 43.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.752 CPU sec, 6.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12338, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 82, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (83.00, 37.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (135.77, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.104 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12298, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 143, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (142.27, 68.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.33, 42.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.497 CPU sec, 6.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12310, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 120, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (104.71, 54.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 42.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.393 CPU sec, 6.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12231, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.18, 42.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.177 CPU sec, 6.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12273, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 101, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (95.91, 55.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.25, 42.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.006 CPU sec, 6.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12050, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.55, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 191.200 CPU sec, 6.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12235, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.24, 43.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 177.871 CPU sec, 7.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12296, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 121, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (90.38, 33.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.35, 43.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.790 CPU sec, 6.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12377, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 67, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (80.00, 43.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.00, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.406 CPU sec, 5.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12252, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 110, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (124.07, 70.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.45, 43.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.155 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12207, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.60, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.330 CPU sec, 6.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12266, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (136.60, 43.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.894 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12301, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 84, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (117.15, 96.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.55, 42.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.805 CPU sec, 6.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12389, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 96, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (94.87, 47.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.78, 42.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.393 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 12424, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (138.12, 44.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.088 CPU sec, 6.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12331, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.83, 42.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.779 CPU sec, 6.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12254, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.62, 42.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.652 CPU sec, 6.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12162, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.86, 42.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.447 CPU sec, 5.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12222, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.50, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 168.236 CPU sec, 6.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12210, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 74, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (77.91, 26.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.27, 42.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.179 CPU sec, 6.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12147, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 101, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (108.50, 63.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.64, 43.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.829 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12277, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 88, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (77.00, 22.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.06, 43.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.342 CPU sec, 7.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12427, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.07, 42.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.345 CPU sec, 5.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12337, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 74, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (39, 109) [M::mem_pestat] mean and std.dev: (72.25, 14.42) [M::mem_pestat] low and high boundaries for proper pairs: (15, 130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.34, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.022 CPU sec, 5.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12620, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 87, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (107.60, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.66, 43.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.899 CPU sec, 5.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12043, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.34, 41.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.524 CPU sec, 6.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12013, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.37, 43.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.667 CPU sec, 6.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12165, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 65, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (67.92, 20.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.15, 42.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.816 CPU sec, 6.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12291, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 135, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (138.26, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 175.327 CPU sec, 7.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12319, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.53, 43.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.744 CPU sec, 6.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12229, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.78, 43.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.424 CPU sec, 6.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12248, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 80, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (75.80, 36.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.54, 43.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.765 CPU sec, 6.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12442, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.39, 43.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.096 CPU sec, 6.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12380, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.11, 43.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.314 CPU sec, 6.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12417, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.25, 43.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 163.549 CPU sec, 5.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12332, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (71.93, 44.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.44, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.095 CPU sec, 7.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12297, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 81, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (79.50, 31.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (136.98, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.901 CPU sec, 6.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12214, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.75, 43.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.077 CPU sec, 5.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12233, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (69.90, 31.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.94, 43.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.650 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12182, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 119, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 202) [M::mem_pestat] mean and std.dev: (105.22, 38.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.48, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.346 CPU sec, 6.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12149, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (138.92, 44.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.521 CPU sec, 5.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12120, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 116, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (132.40, 55.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.15, 43.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.247 CPU sec, 8.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12198, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.32, 42.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.825 CPU sec, 5.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12390, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 88, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (104.67, 52.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.67, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.169 CPU sec, 5.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12427, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.15, 41.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.360 CPU sec, 5.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12402, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.07, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.667 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12128, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 62, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (73.80, 39.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.12, 42.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.632 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12277, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 105, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (115.73, 48.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.21, 43.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.102 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12253, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 106, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (199.09, 179.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.34, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.124 CPU sec, 6.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12312, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 272) [M::mem_pestat] mean and std.dev: (136.64, 41.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 170.318 CPU sec, 6.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12209, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.48, 42.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.150 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12306, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 93, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (92.75, 30.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (137.08, 43.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.812 CPU sec, 6.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12341, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 92, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (117.00, 64.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.03, 43.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.232 CPU sec, 6.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12314, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.91, 43.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.721 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12204, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.71, 43.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 155.553 CPU sec, 5.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12299, 8, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.12, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.045 CPU sec, 5.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12268, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.35, 42.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.363 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12314, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (137.78, 44.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.588 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12275, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (102.10, 36.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.20, 43.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.985 CPU sec, 6.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12280, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.17, 42.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.905 CPU sec, 7.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12272, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.49, 44.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.966 CPU sec, 6.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12151, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 67, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (73.92, 35.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.66, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.295 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12264, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (119.09, 78.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.57, 43.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.323 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12443, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 63, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (80.36, 39.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.45, 43.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.305 CPU sec, 6.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12319, 7, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.12, 42.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.585 CPU sec, 7.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12326, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 87, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (93.92, 48.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.22, 43.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.953 CPU sec, 5.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12366, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 82, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (148.15, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.79, 42.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.621 CPU sec, 6.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12314, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.52, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.114 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12077, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 105, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (101.58, 50.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 131, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (134.82, 42.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.637 CPU sec, 6.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12088, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.25, 42.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.681 CPU sec, 6.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 11974, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.51, 42.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 189.004 CPU sec, 6.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12174, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 93, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (87.80, 35.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.09, 43.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.979 CPU sec, 6.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12120, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (113.42, 67.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.23, 43.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.434 CPU sec, 7.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12324, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 92, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (90.33, 34.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.27, 43.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.481 CPU sec, 6.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12129, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.92, 42.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.834 CPU sec, 6.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12276, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.48, 42.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.259 CPU sec, 7.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12492, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 83, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (93.94, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.84, 43.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.468 CPU sec, 5.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12045, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (38, 51, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (53.40, 28.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.71, 42.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.486 CPU sec, 7.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12251, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 114, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (123.80, 42.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.10, 43.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.904 CPU sec, 7.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12233, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 112, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (136.75, 95.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.66, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.714 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12224, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (136.94, 43.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 169.624 CPU sec, 6.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12167, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.33, 43.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 153.689 CPU sec, 5.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12146, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 82, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (77.30, 34.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.93, 42.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.201 CPU sec, 6.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12069, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.78, 44.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 173.131 CPU sec, 6.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12368, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 72, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (77.27, 45.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.66, 44.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.792 CPU sec, 6.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12393, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 155, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (168.30, 107.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.12, 42.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.856 CPU sec, 5.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12482, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.12, 41.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.869 CPU sec, 4.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12480, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.09, 42.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.641 CPU sec, 5.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12444, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 132, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 245) [M::mem_pestat] mean and std.dev: (124.50, 36.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.96, 43.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.024 CPU sec, 5.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12291, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 75, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (109.60, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.46, 42.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.766 CPU sec, 4.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12037, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 166, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (191.17, 129.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (135.81, 42.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.080 CPU sec, 5.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12101, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 89, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (127.56, 106.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (135.78, 42.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.297 CPU sec, 5.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12051, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.88, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.951 CPU sec, 5.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12322, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 94, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (108.20, 70.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.84, 44.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.028 CPU sec, 4.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 12365, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.61, 43.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 137.149 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12256, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.46, 42.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.919 CPU sec, 4.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12193, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 66, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (97.92, 57.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.82, 43.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.769 CPU sec, 4.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12156, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 89, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (93.17, 46.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.08, 41.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.221 CPU sec, 7.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12209, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 76, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (84.25, 52.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.23, 42.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.520 CPU sec, 6.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12270, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 121, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (89.56, 54.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.07, 42.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.102 CPU sec, 6.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12191, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.25, 43.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.979 CPU sec, 5.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12382, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.63, 42.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 152.925 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12283, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 72, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (89.38, 56.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.23, 42.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.057 CPU sec, 6.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12141, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.96, 42.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 159.977 CPU sec, 6.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12161, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.18, 43.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.053 CPU sec, 6.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12146, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 65, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (91.10, 49.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (137.04, 42.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.651 CPU sec, 6.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12302, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.72, 42.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 154.428 CPU sec, 5.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12292, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (41, 65, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (61.09, 24.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.97, 42.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.969 CPU sec, 6.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12396, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 87, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (112.75, 70.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.64, 42.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.180 CPU sec, 6.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12473, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.30, 42.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 147.946 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12362, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 159, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (126.30, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.14, 42.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.177 CPU sec, 6.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12208, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (135.24, 41.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 145.268 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12264, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 57, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (93.40, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 132, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (135.66, 41.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.416 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 12124, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (136.21, 42.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 160.373 CPU sec, 5.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 12192, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.31, 42.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 158.106 CPU sec, 6.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12174, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (95.60, 42.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.11, 43.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.380 CPU sec, 5.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12203, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.80, 42.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.954 CPU sec, 6.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12191, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (136.69, 41.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 150.354 CPU sec, 5.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12337, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (136.58, 43.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.241 CPU sec, 5.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12351, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.16, 42.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.501 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12322, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (135.98, 41.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 144.188 CPU sec, 6.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12333, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 101, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (115.70, 54.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.03, 43.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.945 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12198, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (115.69, 62.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (138.21, 43.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.504 CPU sec, 5.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 12301, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.46, 43.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 156.415 CPU sec, 6.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12141, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 134, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (137.59, 43.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 146.055 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12404, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 82, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (111.75, 66.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 134, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (137.54, 43.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.153 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12302, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 118, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (101.18, 40.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (137.40, 43.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.098 CPU sec, 6.521 real sec [M::process] read 48706 sequences (7354606 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12287, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 83, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (80.14, 31.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (136.58, 41.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.510 CPU sec, 5.497 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 9055, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (136.31, 42.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 48706 reads in 115.272 CPU sec, 5.582 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21181\tSM:UG10S2P499\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21181_HLKNYBGXX_UG10S2P499_R1.fastq.gz 5347_7332_21181_HLKNYBGXX_UG10S2P499_R2.fastq.gz [main] Real time: 3289.279 sec; CPU: 84471.651 sec Finished alignement of UG10S2P499 at Wed Apr 3 17:10:05 2019 Sorting BAM file started at Wed Apr 3 17:10:05 2019 samtools sort -@ 30 -m 4G UG10S2P499_unsorted.bam -o UG10S2P499.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 17:10:05 2019 rm UG10S2P499_unsorted.bam rm 5347_7332_21181_HLKNYBGXX_UG10S2P499_R1.fastq.gz rm 5347_7332_21181_HLKNYBGXX_UG10S2P499_R2.fastq.gz samtools index UG10S2P499.bam Finished BAM file generation of the sample - UG10S2P499 Processing fastq files for the sample - UG10S1P043 Reading 5347_7332_21182_HLKNYBGXX_UG10S1P043_R1.fastq.gz 5347_7332_21182_HLKNYBGXX_UG10S1P043_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21182\tSM:UG10S1P043\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21182_HLKNYBGXX_UG10S1P043_R1.fastq.gz 5347_7332_21182_HLKNYBGXX_UG10S1P043_R2.fastq.gz | samtools view -Sb1 - > UG10S1P043_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12559, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.11, 59.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 166.783 CPU sec, 6.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12396, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (143.20, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 167.710 CPU sec, 6.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12288, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 95, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (106.69, 71.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (143.16, 62.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.828 CPU sec, 5.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12552, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (140.79, 59.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.513 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12709, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (168.13, 124.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.35, 59.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.293 CPU sec, 5.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12519, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 86, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (112.77, 54.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (143.18, 61.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.265 CPU sec, 6.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12626, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (40, 81, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (60.20, 39.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (140.28, 58.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.138 CPU sec, 6.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12375, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (128.67, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.18, 59.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.538 CPU sec, 5.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12433, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 99, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (117.41, 69.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (143.26, 61.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.035 CPU sec, 5.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12581, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (29, 80, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (104.71, 91.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (140.50, 58.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.450 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12693, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (55.67, 38.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (141.17, 60.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.725 CPU sec, 5.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12507, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 80, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (90.14, 57.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.70, 60.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.610 CPU sec, 6.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12449, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 80, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (106.25, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (142.17, 61.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.958 CPU sec, 7.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12425, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 126, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (123.91, 51.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (141.73, 60.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.394 CPU sec, 6.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12634, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 87, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (90.50, 47.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (140.00, 58.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.569 CPU sec, 6.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12435, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 156, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (147.61, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (140.62, 59.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.375 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12359, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 245, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (239.27, 139.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (139.64, 57.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.916 CPU sec, 5.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12348, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 100, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (117.10, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (142.70, 61.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.725 CPU sec, 6.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12229, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 158, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (174.60, 150.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.35, 59.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.266 CPU sec, 7.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12320, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.15, 58.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 186.556 CPU sec, 6.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12499, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 203, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (163.00, 82.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.82, 60.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.964 CPU sec, 7.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12307, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (101.90, 63.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (138.26, 56.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.886 CPU sec, 6.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12376, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 91, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (96.50, 39.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.27, 58.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.936 CPU sec, 7.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12341, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 204, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (263.83, 176.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (140.68, 59.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.857 CPU sec, 7.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12267, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 132, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (133.17, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (139.64, 57.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.862 CPU sec, 8.947 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12773, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 79, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (79.00, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (140.78, 58.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.208 CPU sec, 7.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12449, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 62, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (76.00, 48.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (139.69, 57.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.207 CPU sec, 6.779 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12299, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 157, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (150.75, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (139.76, 57.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.610 CPU sec, 6.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12381, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 76, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (84.54, 59.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (139.92, 58.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.646 CPU sec, 8.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12141, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 238, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (170.91, 110.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (140.51, 57.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 203.837 CPU sec, 7.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12314, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (141.15, 59.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 208.218 CPU sec, 7.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12456, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 88, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (141.31, 106.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (140.28, 58.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.771 CPU sec, 6.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12491, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 102, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (118.53, 63.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (140.37, 58.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.911 CPU sec, 7.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12233, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 154, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (179.10, 118.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (141.85, 60.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.051 CPU sec, 7.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12302, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 123, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (161.40, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (141.20, 60.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.740 CPU sec, 8.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12419, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 104, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (100.20, 68.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.18, 59.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.723 CPU sec, 7.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12742, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 111, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (85.53, 36.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (137.85, 56.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.499 CPU sec, 6.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12414, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 61, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (125.56, 114.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (138.64, 56.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.433 CPU sec, 6.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12314, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (110.00, 63.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (142.95, 61.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.716 CPU sec, 7.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12094, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 125, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (164.73, 150.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (143.25, 62.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.646 CPU sec, 7.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12409, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (144.57, 63.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 190.359 CPU sec, 6.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12548, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 92, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (100.40, 59.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (140.10, 59.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.547 CPU sec, 6.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12323, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (155.29, 114.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (139.02, 56.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.303 CPU sec, 7.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12360, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (106.00, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (143.60, 62.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.623 CPU sec, 7.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12208, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (124.86, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (142.55, 61.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.558 CPU sec, 7.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12255, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 172, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (153.00, 75.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (141.50, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.542 CPU sec, 8.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12613, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 123, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (134.23, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (139.64, 58.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.895 CPU sec, 6.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12595, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 73, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (80.43, 42.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (138.69, 56.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.831 CPU sec, 6.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12305, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.06, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 202.612 CPU sec, 7.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12290, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (37, 84, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (95.77, 72.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (139.44, 57.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.384 CPU sec, 7.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12150, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (120.42, 72.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (141.24, 59.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.938 CPU sec, 8.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12444, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 138, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (149.93, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 132, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (139.63, 58.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.650 CPU sec, 7.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12677, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (136.76, 54.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 164.948 CPU sec, 6.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12184, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 78, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (68.45, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (140.34, 57.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.090 CPU sec, 6.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12387, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 78, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (144.15, 121.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (140.09, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.250 CPU sec, 7.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12296, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 94, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (100.83, 46.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (141.97, 60.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.096 CPU sec, 8.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12287, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 131, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (127.80, 85.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (141.85, 61.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.508 CPU sec, 7.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12720, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 96, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (128.94, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (139.72, 57.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.438 CPU sec, 6.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12261, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (153.82, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (138.06, 56.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.612 CPU sec, 6.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11972, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 79, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (76.45, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.05, 59.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.108 CPU sec, 8.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12088, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 95, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (81.33, 23.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (141.89, 60.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.733 CPU sec, 8.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12170, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 158, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (290.10, 365.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (143.07, 61.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.819 CPU sec, 7.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12277, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 150, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (143.25, 55.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (138.42, 56.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.331 CPU sec, 7.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12625, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 85, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (77.82, 35.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (136.79, 53.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.083 CPU sec, 6.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12682, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (139.32, 56.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 171.390 CPU sec, 6.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12663, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 111, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (103.38, 53.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (139.98, 57.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.977 CPU sec, 6.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12369, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 68, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (75.00, 39.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.66, 60.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.413 CPU sec, 7.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12366, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (132.75, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (139.46, 58.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.130 CPU sec, 6.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12466, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 118, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (139.82, 92.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.46, 58.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.153 CPU sec, 7.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12641, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 130, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (117.83, 61.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (140.81, 58.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.076 CPU sec, 6.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12754, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 176, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (159.08, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (139.29, 57.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.011 CPU sec, 6.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12466, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 158, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2045) [M::mem_pestat] mean and std.dev: (257.67, 243.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.72, 60.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 212.982 CPU sec, 7.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12338, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 99, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (106.23, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (140.58, 60.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.728 CPU sec, 6.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12546, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 115, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (159.45, 95.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (140.42, 57.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.880 CPU sec, 6.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12574, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 178, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (180.69, 105.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (140.54, 58.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.013 CPU sec, 6.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12670, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (132.44, 89.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (139.06, 56.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.342 CPU sec, 7.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12401, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 78, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (107.05, 90.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.88, 60.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.281 CPU sec, 7.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12539, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 112, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (109.47, 39.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (139.01, 57.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.639 CPU sec, 7.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12534, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (152.45, 76.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (138.66, 56.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.257 CPU sec, 8.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12388, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 73, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (100.88, 65.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (142.19, 60.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.880 CPU sec, 7.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12658, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 74, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (86.67, 64.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.07, 58.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.982 CPU sec, 8.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11982, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (112.83, 63.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (139.21, 57.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.896 CPU sec, 7.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12214, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 111, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (158.30, 111.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (139.67, 57.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.473 CPU sec, 7.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12544, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 211, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (154.36, 107.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (137.55, 55.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.452 CPU sec, 6.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12330, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 81, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (109.15, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (141.77, 60.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.136 CPU sec, 8.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12751, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 90, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (126.27, 102.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (140.67, 58.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.564 CPU sec, 6.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12413, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 128, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (167.15, 111.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.25, 58.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.369 CPU sec, 6.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12424, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 78, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (75.26, 39.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (139.87, 57.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 206.050 CPU sec, 8.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12529, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (148.76, 111.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (140.48, 57.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.610 CPU sec, 7.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12427, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (139.72, 57.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 192.666 CPU sec, 7.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12568, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (141.07, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 182.361 CPU sec, 7.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12590, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 103, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (129.55, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (140.11, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.281 CPU sec, 6.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12336, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 102, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (139.93, 94.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (141.68, 60.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.765 CPU sec, 7.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12450, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 128, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (128.00, 84.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (138.08, 56.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 199.441 CPU sec, 7.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12546, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 161, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (231.71, 204.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (138.95, 56.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.367 CPU sec, 6.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12522, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 101, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (164.19, 132.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (139.97, 57.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.311 CPU sec, 6.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12721, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 175, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (186.00, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (138.88, 55.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.804 CPU sec, 6.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12423, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 141, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (164.21, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (141.64, 60.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 202.743 CPU sec, 7.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12623, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 129, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (123.36, 70.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (142.08, 61.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.937 CPU sec, 7.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12323, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 109, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (137.18, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (141.37, 60.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.501 CPU sec, 7.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12496, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (162.25, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (140.76, 58.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 200.338 CPU sec, 7.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12720, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 151, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (160.94, 91.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (138.35, 55.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.486 CPU sec, 6.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12371, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 118, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (153.53, 139.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (141.11, 60.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 213.386 CPU sec, 7.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12394, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 133, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (141.17, 60.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 199.124 CPU sec, 7.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12250, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (133.08, 83.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (142.68, 61.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.517 CPU sec, 6.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12345, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (159.06, 105.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (142.14, 60.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.621 CPU sec, 7.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12442, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 80, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (129.57, 105.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 131, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (137.39, 55.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.861 CPU sec, 6.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12369, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 179, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (204.78, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.70, 59.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.510 CPU sec, 6.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12433, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 88, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (98.27, 63.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (142.55, 59.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.359 CPU sec, 6.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12404, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 166, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (157.36, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 133, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (140.94, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.475 CPU sec, 6.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12407, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 158, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (234.73, 218.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (142.45, 61.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.564 CPU sec, 6.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12465, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 100, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (147.39, 100.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 132, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (138.57, 56.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.701 CPU sec, 7.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12300, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 65, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (81.06, 56.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 131, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (138.23, 55.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.216 CPU sec, 8.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12305, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (127.86, 95.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (138.82, 55.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.624 CPU sec, 7.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12272, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (133.75, 64.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (141.17, 59.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.503 CPU sec, 6.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12222, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 84, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (70.38, 28.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (139.46, 57.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.489 CPU sec, 7.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 95, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (121.60, 77.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (136.67, 53.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.183 CPU sec, 7.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12233, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 123, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (164.18, 99.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 130, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (135.76, 52.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.304 CPU sec, 7.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12256, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (24, 81, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (107.80, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (142.55, 60.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.672 CPU sec, 8.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 12376, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (137.75, 55.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 176.735 CPU sec, 6.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12411, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 104, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (144.50, 121.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (140.99, 59.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.662 CPU sec, 6.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12356, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 106, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (139.92, 100.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (139.59, 57.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.832 CPU sec, 5.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12482, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 102, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (122.79, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (137.72, 55.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.299 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12336, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 90, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (114.30, 80.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (106, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (140.28, 57.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.415 CPU sec, 6.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12245, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.71, 58.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.339 CPU sec, 6.059 real sec [M::process] read 40436 sequences (6105836 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12224, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 72, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (72.00, 31.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (140.89, 59.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.030 CPU sec, 7.843 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 7561, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (140.14, 58.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 40436 reads in 125.685 CPU sec, 4.638 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21182\tSM:UG10S1P043\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21182_HLKNYBGXX_UG10S1P043_R1.fastq.gz 5347_7332_21182_HLKNYBGXX_UG10S1P043_R2.fastq.gz [main] Real time: 893.400 sec; CPU: 24083.041 sec Finished alignement of UG10S1P043 at Wed Apr 3 18:07:47 2019 Sorting BAM file started at Wed Apr 3 18:07:47 2019 samtools sort -@ 30 -m 4G UG10S1P043_unsorted.bam -o UG10S1P043.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 18:07:47 2019 rm UG10S1P043_unsorted.bam rm 5347_7332_21182_HLKNYBGXX_UG10S1P043_R1.fastq.gz rm 5347_7332_21182_HLKNYBGXX_UG10S1P043_R2.fastq.gz samtools index UG10S1P043.bam Finished BAM file generation of the sample - UG10S1P043 Processing fastq files for the sample - UG10S2P518 Reading 5347_7332_21183_HLKNYBGXX_UG10S2P518_R1.fastq.gz 5347_7332_21183_HLKNYBGXX_UG10S2P518_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21183\tSM:UG10S2P518\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21183_HLKNYBGXX_UG10S2P518_R1.fastq.gz 5347_7332_21183_HLKNYBGXX_UG10S2P518_R2.fastq.gz | samtools view -Sb1 - > UG10S2P518_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13466, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 186, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (233.60, 145.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (254.38, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.826 CPU sec, 7.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13617, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 169, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (170.60, 110.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (253.24, 133.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.352 CPU sec, 6.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13722, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 155, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (182.94, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.81, 135.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.930 CPU sec, 6.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13853, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 142, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (163.84, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (256.77, 135.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.978 CPU sec, 5.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13907, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 146, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (198.52, 149.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (257.36, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.069 CPU sec, 6.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13968, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (187.54, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (256.56, 136.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.066 CPU sec, 7.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14091, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 124, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (161.24, 99.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (257.52, 136.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.271 CPU sec, 6.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13980, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 173, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (173.73, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (259.12, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.661 CPU sec, 6.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14041, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 201, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (190.00, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (254.65, 136.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.843 CPU sec, 7.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13905, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 212, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (201.09, 102.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (259.51, 139.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.673 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13908, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 143, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (141.45, 72.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (259.13, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.783 CPU sec, 6.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13995, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 197, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (254.63, 181.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (259.55, 138.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.887 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13890, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 151, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (164.50, 53.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 223, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (259.26, 138.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.084 CPU sec, 6.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13798, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (152.10, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (257.35, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.375 CPU sec, 7.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14104, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 179, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (208.87, 123.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (257.99, 139.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.942 CPU sec, 5.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13852, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 152, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (182.70, 128.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (256.50, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.227 CPU sec, 5.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13818, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 184, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (180.43, 110.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (255.04, 136.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.214 CPU sec, 6.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13853, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 136, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (129.17, 63.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (255.13, 136.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.922 CPU sec, 6.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13833, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 188, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (190.12, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (256.88, 136.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.862 CPU sec, 6.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13915, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 155, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (167.12, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.12, 134.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.228 CPU sec, 5.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13952, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (205.79, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (253.51, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.401 CPU sec, 5.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13984, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 165, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (170.82, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (251.27, 133.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.212 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14084, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 152, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (153.71, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (249.46, 132.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.366 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 14026, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 181, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (191.94, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (250.48, 132.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.492 CPU sec, 6.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13979, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 177, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (197.65, 93.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (251.04, 132.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.333 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13935, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 158, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (182.05, 91.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.36, 130.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.766 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13856, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (185.13, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (249.07, 132.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.938 CPU sec, 5.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13897, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 121, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (170.06, 114.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (247.89, 130.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.285 CPU sec, 5.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13935, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (166.20, 92.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (248.84, 131.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.358 CPU sec, 6.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13914, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (191.91, 98.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (246.43, 130.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.582 CPU sec, 6.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13574, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 166, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (166.18, 56.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (254.31, 134.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.265 CPU sec, 6.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13587, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 149, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (140.06, 63.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (254.38, 133.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.948 CPU sec, 6.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13770, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 175, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (186.53, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.08, 134.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.431 CPU sec, 6.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13722, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 200, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (186.64, 87.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (253.35, 134.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.614 CPU sec, 8.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13650, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 126, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (150.32, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (256.42, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.560 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13901, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 192, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (193.44, 93.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (254.11, 135.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.928 CPU sec, 7.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14038, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 189, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (215.21, 133.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.42, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.061 CPU sec, 6.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13811, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 223, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (245.67, 143.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (257.48, 136.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.651 CPU sec, 7.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13711, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 154, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (143.69, 70.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (256.24, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.207 CPU sec, 6.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13999, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 151, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (174.32, 107.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (256.08, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.720 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13920, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (177.00, 112.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (258.81, 138.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.688 CPU sec, 5.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13964, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 164, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (205.07, 114.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (257.79, 138.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.735 CPU sec, 6.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14007, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 231, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (251.81, 174.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (255.82, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.893 CPU sec, 5.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13926, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 165, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (185.94, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 223, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (257.83, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.157 CPU sec, 6.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14089, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 204, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (201.36, 105.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 225, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (259.87, 139.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.799 CPU sec, 6.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13922, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 215, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (229.72, 112.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (255.06, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.308 CPU sec, 6.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14085, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 164, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (185.38, 156.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (253.41, 135.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.496 CPU sec, 6.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13923, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 168, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (163.00, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (253.90, 134.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.877 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13999, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (132.67, 66.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (254.59, 136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.220 CPU sec, 7.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13969, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 177, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (174.94, 82.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (254.71, 134.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.300 CPU sec, 6.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14096, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 134, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (161.64, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (254.51, 134.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.381 CPU sec, 6.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13961, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 194, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (205.00, 101.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (250.90, 132.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.006 CPU sec, 6.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14115, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 213, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (210.33, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (252.14, 134.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.929 CPU sec, 5.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14124, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 179, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (212.33, 106.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (248.59, 132.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.731 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13990, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (172.95, 100.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (247.38, 131.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.102 CPU sec, 6.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13894, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (200.54, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (248.86, 131.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.553 CPU sec, 6.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13831, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 165, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (148.59, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (244.90, 129.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.348 CPU sec, 6.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13807, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 179, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (186.80, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (246.53, 131.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.675 CPU sec, 7.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13885, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 227, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (227.54, 114.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.15, 130.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.100 CPU sec, 6.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13935, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 239, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (248.17, 128.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.92, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.166 CPU sec, 6.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13658, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (183.80, 60.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (251.75, 133.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.369 CPU sec, 7.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13532, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 175, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (184.52, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 216, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (253.94, 134.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.699 CPU sec, 7.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13704, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 163, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (200.75, 122.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (254.48, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.341 CPU sec, 7.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13846, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (138.86, 59.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.74, 134.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.002 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13838, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (151.00, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.06, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.329 CPU sec, 5.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13762, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 220, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (212.07, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (255.71, 136.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.713 CPU sec, 5.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13840, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 175, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (165.63, 105.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.48, 135.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.343 CPU sec, 6.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13797, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 144, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (155.94, 77.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 222, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (257.62, 137.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.861 CPU sec, 5.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13820, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 153, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (145.47, 86.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (253.25, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.728 CPU sec, 6.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13972, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 157, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (175.61, 105.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (256.40, 135.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.625 CPU sec, 6.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13784, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 153, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (170.00, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (257.82, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.240 CPU sec, 6.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13827, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 173, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (178.78, 110.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (255.54, 136.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.180 CPU sec, 6.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13834, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.25, 113.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (255.70, 135.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.162 CPU sec, 6.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13792, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 201, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (227.54, 191.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.33, 137.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.803 CPU sec, 7.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13729, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 226, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (202.56, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (253.99, 135.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.420 CPU sec, 7.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13869, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 200, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (204.56, 130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (252.90, 134.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.376 CPU sec, 6.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14028, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 190, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (184.62, 143.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (252.16, 134.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.786 CPU sec, 7.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13878, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 173, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (191.47, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (250.64, 133.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.946 CPU sec, 6.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13951, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 140, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (148.06, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.75, 134.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.822 CPU sec, 5.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13846, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 128, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (133.17, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (250.34, 133.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.128 CPU sec, 6.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14031, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 150, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (177.55, 101.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (250.45, 133.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.854 CPU sec, 6.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13904, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 100, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (114.19, 48.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (247.92, 131.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.348 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13848, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 197, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (182.26, 80.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (245.89, 129.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.945 CPU sec, 5.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13946, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 178, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (182.90, 108.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (247.22, 131.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.869 CPU sec, 6.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13941, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 261, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (221.88, 100.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.60, 129.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.493 CPU sec, 6.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13902, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (181.94, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (246.59, 130.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.723 CPU sec, 7.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13925, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 148, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (143.67, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (246.11, 129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.411 CPU sec, 5.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13928, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 180, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (167.53, 88.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (245.00, 129.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.180 CPU sec, 6.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13890, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 200, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (204.58, 108.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (243.89, 129.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.959 CPU sec, 5.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13731, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 164, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (166.85, 94.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.49, 128.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.715 CPU sec, 5.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13583, 12, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 157, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (160.65, 64.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (244.17, 128.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 1841, 6532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19102) [M::mem_pestat] mean and std.dev: (2945.08, 3122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25387) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 153.100 CPU sec, 6.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13556, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 156, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (154.12, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (243.69, 128.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.557 CPU sec, 5.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13562, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 230, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (239.48, 182.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (250.92, 133.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.193 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13805, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 193, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (175.92, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (248.21, 131.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.902 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13898, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 108, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (153.15, 100.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (249.46, 133.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.873 CPU sec, 6.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13841, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 153, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (179.46, 103.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.96, 134.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.924 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13929, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 172, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (167.56, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (254.46, 135.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.837 CPU sec, 5.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14031, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 132, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (148.77, 82.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (255.54, 136.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.482 CPU sec, 6.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13854, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 121, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (140.15, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (256.69, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.026 CPU sec, 5.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13871, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 140, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (184.88, 122.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.89, 135.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.788 CPU sec, 6.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13870, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 185, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (215.47, 113.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.08, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.874 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13883, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 182, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (166.76, 81.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (256.58, 136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.467 CPU sec, 7.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13951, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 162, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (159.50, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.16, 136.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.192 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13941, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 163, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (166.05, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (254.82, 135.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.614 CPU sec, 6.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13770, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (140.96, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (254.77, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.784 CPU sec, 5.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 14043, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (118.11, 69.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (252.95, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.758 CPU sec, 7.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13901, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 149, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (146.32, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 216, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (251.70, 132.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.254 CPU sec, 6.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14096, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 104, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (151.26, 109.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (251.51, 134.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.923 CPU sec, 5.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13946, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (148.41, 88.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (249.53, 132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.876 CPU sec, 6.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13911, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 118, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (169.52, 110.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (248.39, 131.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.876 CPU sec, 6.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13936, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 189, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (179.53, 121.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (247.26, 131.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.930 CPU sec, 4.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13921, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (191.55, 94.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (245.45, 130.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.627 CPU sec, 6.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13715, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 176, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (174.57, 111.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.05, 128.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.644 CPU sec, 6.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13889, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 202, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (195.62, 122.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (248.34, 130.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.332 CPU sec, 5.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13982, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (163.77, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (245.54, 129.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.475 CPU sec, 6.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13991, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (132.92, 58.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (244.01, 129.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.111 CPU sec, 6.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13883, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 133, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (159.22, 102.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (244.61, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.503 CPU sec, 5.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13841, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 231, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (178.08, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (243.10, 127.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.756 CPU sec, 6.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13804, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 151, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (196.32, 148.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (244.29, 127.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.420 CPU sec, 6.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13813, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 182, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (168.46, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (244.31, 128.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.674 CPU sec, 6.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13471, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 203, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (231.78, 125.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (242.16, 128.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.057 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13504, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 201, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (168.71, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (244.35, 129.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.950 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13535, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 215, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (225.00, 121.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (247.31, 131.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.720 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13659, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 117, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (135.61, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.84, 130.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.790 CPU sec, 6.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13851, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 190, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (203.00, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (249.99, 131.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.322 CPU sec, 6.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13799, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 188, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (177.64, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 213, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (249.82, 134.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.977 CPU sec, 6.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13883, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 128, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (130.91, 59.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (251.32, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.793 CPU sec, 6.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13983, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 120, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (147.29, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.01, 135.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.955 CPU sec, 6.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13965, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 150, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (154.00, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.79, 135.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.681 CPU sec, 6.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14006, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 119, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (167.22, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (252.21, 134.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.346 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13875, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 216, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (164.40, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.40, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.998 CPU sec, 5.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13769, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 189, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (191.20, 110.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (253.64, 134.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.819 CPU sec, 5.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14036, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 114, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (151.67, 92.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.81, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.324 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13840, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 199, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (213.24, 109.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (250.69, 132.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.778 CPU sec, 7.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14030, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 179, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (198.95, 157.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (253.96, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.918 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14062, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 208, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (191.18, 112.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (252.09, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.177 CPU sec, 6.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13992, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 202, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (203.60, 133.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.56, 132.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.952 CPU sec, 5.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13734, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 106, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (165.76, 124.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (250.64, 133.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.128 CPU sec, 6.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13837, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 176, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (180.00, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (249.72, 132.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.468 CPU sec, 5.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13949, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (159.25, 73.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.53, 133.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.414 CPU sec, 5.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13967, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 172, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (183.00, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (249.06, 132.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.478 CPU sec, 5.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13827, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 153, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (153.54, 92.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (244.68, 128.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.513 CPU sec, 6.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13999, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 155, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (162.60, 103.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 209, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (245.22, 129.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.368 CPU sec, 6.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13941, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 176, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (164.05, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (247.13, 130.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.591 CPU sec, 5.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13840, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 139, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (168.77, 100.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (247.58, 130.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.564 CPU sec, 5.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13864, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 155, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (154.00, 85.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (244.60, 128.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.361 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13989, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 178, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (176.04, 104.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (244.05, 127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.466 CPU sec, 4.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13933, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 160, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (193.00, 121.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (241.67, 127.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.883 CPU sec, 6.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13754, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 240, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (226.75, 102.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (245.64, 129.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.804 CPU sec, 6.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13790, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 144, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (170.48, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (243.36, 128.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.502 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13356, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 189, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (222.14, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.54, 130.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.915 CPU sec, 7.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13578, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 141, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (182.44, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (244.46, 129.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.038 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13477, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 102, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (116.18, 64.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.01, 129.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.130 CPU sec, 6.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 126, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (148.20, 87.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (249.69, 132.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.648 CPU sec, 6.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13715, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 199, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (204.21, 126.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (249.60, 133.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.818 CPU sec, 6.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13835, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 164, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (147.95, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (249.58, 132.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.311 CPU sec, 5.994 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13804, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 159, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (154.30, 88.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (247.72, 133.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.955 CPU sec, 5.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13832, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 127, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (157.77, 109.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (251.51, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.459 CPU sec, 5.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13924, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 135, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (147.14, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (251.24, 134.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.046 CPU sec, 5.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13850, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (122.31, 61.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (252.70, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.899 CPU sec, 6.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13763, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 161, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (177.19, 111.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (251.08, 134.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.343 CPU sec, 6.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13833, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 169, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (175.22, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (250.69, 133.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.898 CPU sec, 6.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13804, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 197, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (215.63, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (254.07, 134.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.673 CPU sec, 5.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13802, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 222, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (217.62, 121.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (250.49, 133.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.934 CPU sec, 6.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13765, 11, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 151, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (182.33, 103.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (246.96, 131.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 2155, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11925) [M::mem_pestat] mean and std.dev: (2966.09, 3275.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16069) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 167.961 CPU sec, 7.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14001, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (192.58, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (249.81, 132.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.669 CPU sec, 7.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13848, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (163.00, 78.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (248.81, 132.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.922 CPU sec, 6.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13799, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 200, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (229.26, 144.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (245.64, 130.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.788 CPU sec, 6.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13641, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 216, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (219.83, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (246.11, 130.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.402 CPU sec, 6.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13716, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 198, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (203.74, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (246.60, 130.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.200 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13936, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 245, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (228.85, 109.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (245.78, 130.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.811 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13931, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 123, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (153.84, 103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (242.62, 127.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.366 CPU sec, 5.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13741, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 149, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (157.46, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.46, 128.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.755 CPU sec, 5.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13770, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 157, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (179.21, 120.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (243.07, 127.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.581 CPU sec, 6.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13861, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 228, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (217.48, 110.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.62, 128.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.195 CPU sec, 6.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13835, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 167, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (174.10, 114.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (243.20, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.048 CPU sec, 6.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13636, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 156, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (168.35, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.89, 127.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.873 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13854, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 168, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (168.35, 89.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (241.42, 127.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.764 CPU sec, 5.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13709, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (145.52, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (241.93, 127.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.323 CPU sec, 6.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13956, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 246, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (224.29, 116.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (251.61, 134.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.242 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13994, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 204, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (186.40, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 224, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (257.97, 136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.058 CPU sec, 6.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13858, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 134, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (186.26, 118.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 223, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (256.81, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.699 CPU sec, 6.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13934, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 191, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (182.28, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (255.71, 135.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.040 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13744, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 214, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (195.15, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (254.56, 134.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.423 CPU sec, 6.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13768, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (226.06, 105.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (257.52, 137.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.244 CPU sec, 6.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14062, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 205, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (194.26, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.74, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.182 CPU sec, 6.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13898, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 233, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (227.14, 145.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (257.15, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.230 CPU sec, 5.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13929, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 231, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (222.21, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 225, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (259.69, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.822 CPU sec, 7.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13958, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 252, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (354.20, 224.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (257.72, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.791 CPU sec, 6.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13982, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 149, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (150.00, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.30, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.452 CPU sec, 5.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13957, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 209, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (218.15, 144.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 222, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (257.61, 136.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.472 CPU sec, 5.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13973, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 272, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (205.62, 108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (257.33, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.953 CPU sec, 6.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13800, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (151.70, 88.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (257.09, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.119 CPU sec, 5.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13897, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 201, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (191.17, 83.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (257.00, 136.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.241 CPU sec, 6.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14047, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 212, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (201.60, 109.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (256.42, 136.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.925 CPU sec, 6.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13869, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 141, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (167.88, 89.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (254.72, 135.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.793 CPU sec, 6.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13740, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 152, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (174.64, 108.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (255.16, 136.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.591 CPU sec, 6.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14041, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 146, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (172.89, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (255.26, 136.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.135 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13929, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 208, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (200.33, 114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.41, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.096 CPU sec, 6.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13829, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 200, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (198.65, 104.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (253.42, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.535 CPU sec, 6.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13958, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 164, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (173.07, 89.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (247.09, 131.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.124 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13883, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 147, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (211.24, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (247.67, 132.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.697 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13811, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 154, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (172.12, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 209, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (243.89, 129.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.235 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13754, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 182, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (197.76, 109.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (244.28, 129.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.287 CPU sec, 6.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13727, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 170, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (201.10, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (243.77, 127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.408 CPU sec, 6.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13656, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 199, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (185.29, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (240.57, 127.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.416 CPU sec, 4.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13522, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 166, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (178.17, 83.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (239.68, 124.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.838 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13469, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 155, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (194.53, 132.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (237.68, 123.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.010 CPU sec, 6.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13197, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 99, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (121.70, 83.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.95, 122.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.382 CPU sec, 5.799 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13210, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 232, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (231.16, 103.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (245.08, 128.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.613 CPU sec, 7.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13882, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (186.77, 93.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (261.93, 140.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.604 CPU sec, 6.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13989, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (167.74, 143.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 227, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (261.25, 137.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.717 CPU sec, 7.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13879, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 152, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (190.90, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (261.17, 138.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.012 CPU sec, 6.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13728, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 171, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (168.34, 85.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 228, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (263.32, 139.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.313 CPU sec, 5.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13852, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 189, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (201.65, 100.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 228, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (261.73, 138.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.847 CPU sec, 6.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13839, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 140, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (160.62, 87.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 226, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (260.87, 138.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.282 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13900, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 187, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (195.21, 96.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 224, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (259.07, 137.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.308 CPU sec, 5.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14137, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 156, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (171.55, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 225, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (261.63, 139.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.974 CPU sec, 6.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14005, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 181, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (208.45, 141.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (259.02, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.852 CPU sec, 5.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14110, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 203, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (247.75, 174.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 224, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (259.21, 137.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.336 CPU sec, 6.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13887, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 116, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (154.00, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (261.20, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.150 CPU sec, 5.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13905, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 207, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (220.96, 85.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (262.30, 139.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.081 CPU sec, 6.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13917, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 186, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (203.11, 121.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (258.26, 136.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.164 CPU sec, 6.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13827, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (235.47, 125.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (257.91, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.081 CPU sec, 6.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14068, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (202.00, 125.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (256.96, 135.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.319 CPU sec, 7.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14083, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 199, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (200.00, 103.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (258.34, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.860 CPU sec, 7.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13927, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 197, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (204.92, 94.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (257.08, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.942 CPU sec, 7.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14012, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 148, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (156.64, 102.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (255.46, 136.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.508 CPU sec, 6.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14097, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 136, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (177.67, 118.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 218, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (257.10, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.943 CPU sec, 5.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13880, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 189, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (178.50, 118.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (254.44, 135.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.652 CPU sec, 6.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 14099, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 146, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (163.90, 110.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (250.09, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.911 CPU sec, 6.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13872, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 148, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (198.15, 102.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (245.70, 129.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.742 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13768, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 169, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (148.32, 44.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (244.07, 130.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.519 CPU sec, 6.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13577, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 144, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (166.38, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.59, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.358 CPU sec, 5.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13739, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 151, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (144.60, 53.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (240.79, 127.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.936 CPU sec, 6.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13718, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 106, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (132.52, 94.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (237.27, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.040 CPU sec, 5.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13439, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 204, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (229.68, 145.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.24, 124.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.321 CPU sec, 5.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13239, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 116, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (157.59, 133.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (239.60, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.398 CPU sec, 7.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12995, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 209, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (178.31, 90.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.68, 123.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.137 CPU sec, 7.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13284, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 170, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (158.09, 85.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (243.10, 129.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.731 CPU sec, 6.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13753, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 185, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (193.31, 111.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 228, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (262.81, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.451 CPU sec, 6.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13953, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (200.38, 98.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 227, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (261.83, 138.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.347 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13989, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 175, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (202.92, 126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 225, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (260.67, 138.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.968 CPU sec, 7.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13777, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 111, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (103.71, 53.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (259.47, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.123 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13934, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 162, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (192.88, 111.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 225, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (260.93, 138.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.856 CPU sec, 6.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13913, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 156, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (151.47, 82.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 223, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (261.53, 140.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.582 CPU sec, 7.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13891, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 160, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (206.95, 116.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (259.45, 138.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.894 CPU sec, 6.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13807, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 168, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (179.93, 105.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 225, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (260.70, 139.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.101 CPU sec, 6.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13857, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 119, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (151.37, 96.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (258.74, 137.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.905 CPU sec, 6.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13831, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 179, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (175.04, 83.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 226, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (259.76, 137.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.721 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13917, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 178, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (168.44, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (260.23, 139.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.088 CPU sec, 5.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13899, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 187, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (196.83, 102.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 223, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (259.51, 138.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.494 CPU sec, 7.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13958, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 222, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (210.81, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (258.62, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.497 CPU sec, 6.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14019, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 181, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (197.46, 111.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (256.78, 135.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.574 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13893, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 154, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (182.44, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (255.52, 136.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.385 CPU sec, 6.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14021, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 163, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (190.05, 107.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 223, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (257.72, 137.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.962 CPU sec, 6.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13847, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 205, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (213.60, 108.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (258.15, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.267 CPU sec, 7.071 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13929, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (253.47, 148.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.60, 134.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.073 CPU sec, 6.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13794, 9, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 216, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (192.94, 106.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (252.37, 134.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.390 CPU sec, 5.789 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13854, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 184, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (165.72, 104.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (249.88, 133.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.746 CPU sec, 6.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13812, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 113, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (128.36, 62.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (248.47, 131.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.650 CPU sec, 5.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13800, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (196.83, 108.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (246.33, 130.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.071 CPU sec, 7.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13791, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (143.70, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (242.65, 128.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.835 CPU sec, 5.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13703, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 193, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (190.61, 113.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (243.74, 129.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.477 CPU sec, 6.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13752, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (203.70, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (238.66, 126.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.645 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13574, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 149, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (163.09, 101.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.41, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.091 CPU sec, 5.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13501, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 158, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (159.54, 96.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (236.51, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.093 CPU sec, 5.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13459, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 172, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (184.30, 111.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (236.61, 123.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.249 CPU sec, 6.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12873, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 162, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (186.46, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.94, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.678 CPU sec, 5.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13066, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (152.19, 66.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (242.17, 128.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.721 CPU sec, 5.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13875, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 151, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (173.73, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (253.67, 133.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.098 CPU sec, 7.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13821, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 141, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (136.62, 76.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (255.21, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.300 CPU sec, 6.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13908, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 189, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (178.00, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (253.09, 133.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.278 CPU sec, 8.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13972, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 190, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (217.50, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (251.51, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.144 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13740, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 176, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (193.71, 107.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (252.09, 133.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.382 CPU sec, 7.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13978, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 99, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (171.17, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.82, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.039 CPU sec, 5.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13857, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 185, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (195.08, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 215, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (252.83, 133.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.713 CPU sec, 5.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13821, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (165.78, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (251.65, 133.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.850 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13945, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 181, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (155.91, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 219, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (253.13, 134.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.286 CPU sec, 6.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13759, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 147, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (171.48, 108.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.34, 133.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.947 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13927, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (158.72, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (254.72, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.560 CPU sec, 6.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13861, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (148.92, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (254.24, 133.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.944 CPU sec, 6.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13842, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 194, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (184.78, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (252.19, 134.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.475 CPU sec, 5.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13914, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (149.17, 79.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (253.81, 134.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.482 CPU sec, 7.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13960, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 142, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (166.73, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.29, 133.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.824 CPU sec, 6.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13996, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 177, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (179.31, 64.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.43, 133.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.611 CPU sec, 7.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13990, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 166, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (177.79, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.62, 133.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.470 CPU sec, 5.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14036, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 141, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (164.23, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (250.51, 133.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.018 CPU sec, 6.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13752, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 135, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (163.53, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (249.88, 132.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.846 CPU sec, 6.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13876, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 153, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (174.28, 102.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (246.67, 131.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.364 CPU sec, 7.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13583, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 179, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (169.70, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (244.90, 130.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.491 CPU sec, 6.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13929, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 178, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (187.64, 100.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.63, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.837 CPU sec, 6.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13625, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 175, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (207.16, 106.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.07, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.210 CPU sec, 6.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13527, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 135, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (165.12, 110.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (237.36, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.518 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13878, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (192.67, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.66, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.818 CPU sec, 6.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13543, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 187, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (209.31, 164.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.80, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.432 CPU sec, 5.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13534, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 172, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (192.10, 132.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.49, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.297 CPU sec, 4.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13300, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 172, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (171.11, 85.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (231.57, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.386 CPU sec, 6.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13039, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 128, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (130.88, 47.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (233.28, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.832 CPU sec, 6.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13677, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 178, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (236.63, 161.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (244.09, 128.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.049 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13988, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 171, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (188.52, 111.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.67, 136.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.344 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14035, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 149, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (156.67, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 222, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (258.36, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.082 CPU sec, 6.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13929, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 247, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (209.64, 122.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (257.55, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.318 CPU sec, 6.655 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13911, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 157, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (141.71, 59.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 222, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (257.88, 136.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.547 CPU sec, 6.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13929, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 221, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (300.43, 301.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 223, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (257.41, 136.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.334 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14053, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 223, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (226.94, 115.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (257.85, 137.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.648 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13933, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 163, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (192.59, 114.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 224, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (258.86, 137.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.156 CPU sec, 7.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13974, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 204, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (208.38, 136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (256.81, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.384 CPU sec, 7.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13925, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 190, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (194.06, 131.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.72, 136.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.306 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13945, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 179, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (186.71, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (254.48, 136.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.263 CPU sec, 6.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14012, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 177, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (172.71, 113.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (255.86, 136.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.618 CPU sec, 5.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14071, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 139, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (162.50, 85.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 223, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (259.68, 137.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.493 CPU sec, 7.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14077, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 176, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 376) [M::mem_pestat] mean and std.dev: (192.71, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (255.42, 136.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.313 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13874, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (158.16, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (256.73, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.431 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14103, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 162, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (160.47, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 224, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (257.63, 136.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.652 CPU sec, 7.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13850, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 114, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (143.67, 90.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (254.96, 135.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.492 CPU sec, 6.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13894, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 152, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (169.08, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.97, 135.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.285 CPU sec, 6.055 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13880, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 130, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (144.18, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.47, 133.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.668 CPU sec, 5.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13834, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 150, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (159.32, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (250.12, 131.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.029 CPU sec, 6.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13849, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (135.05, 88.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (247.78, 131.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.572 CPU sec, 5.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13760, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 122, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (156.17, 109.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.99, 128.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.153 CPU sec, 6.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13796, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 164, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (150.89, 90.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.39, 126.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.832 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13790, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 127, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (168.24, 112.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (243.25, 128.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.550 CPU sec, 5.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13795, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 148, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (173.19, 125.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.33, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.328 CPU sec, 5.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13899, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 148, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (165.15, 87.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (238.83, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.905 CPU sec, 6.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13527, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (173.77, 86.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (236.59, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.583 CPU sec, 5.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13335, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 184, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (156.33, 53.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.16, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.520 CPU sec, 5.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13180, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 143, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (169.76, 107.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (229.32, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.760 CPU sec, 5.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12973, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 140, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (144.30, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (231.11, 120.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.882 CPU sec, 7.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13642, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 176, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (209.60, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (254.91, 134.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.901 CPU sec, 5.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13944, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 141, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (161.60, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 222, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (258.43, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.053 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13970, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 138, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (186.24, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 226, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (260.29, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.464 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13789, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 183, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (197.55, 130.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (258.52, 137.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.795 CPU sec, 6.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13857, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 165, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (169.58, 100.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (257.47, 135.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.502 CPU sec, 6.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13670, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 192, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (182.88, 100.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (256.03, 135.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.234 CPU sec, 6.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13877, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 233, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (240.36, 140.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (256.31, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.794 CPU sec, 6.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14040, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 174, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (159.06, 66.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (254.55, 136.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.657 CPU sec, 6.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13867, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 225, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (225.96, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (254.82, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.497 CPU sec, 6.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13938, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 156, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (180.00, 112.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (252.57, 133.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.163 CPU sec, 7.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13973, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 172, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (173.18, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (255.85, 135.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.825 CPU sec, 7.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13852, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 199, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (198.12, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (254.00, 135.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.111 CPU sec, 5.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13839, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 142, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (168.43, 109.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (254.59, 136.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.128 CPU sec, 7.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13778, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 131, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (176.71, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (253.59, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.315 CPU sec, 7.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13662, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 193, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (199.73, 96.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (255.17, 134.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.002 CPU sec, 7.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13815, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 244, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (275.86, 134.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (252.60, 133.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.844 CPU sec, 6.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13896, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 174, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (173.64, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (252.72, 134.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.660 CPU sec, 6.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13886, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 128, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (132.73, 58.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (249.02, 131.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.026 CPU sec, 7.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13921, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 151, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (169.45, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (249.46, 132.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.308 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13826, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (190.68, 110.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (248.14, 132.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.055 CPU sec, 6.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13967, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 226, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (200.74, 97.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (244.38, 129.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.113 CPU sec, 6.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13718, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 175, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (204.96, 128.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.02, 126.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.815 CPU sec, 6.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13669, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (187.77, 93.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.93, 127.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.591 CPU sec, 5.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13791, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 110, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (148.89, 98.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (238.83, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.950 CPU sec, 7.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13689, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 164, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (156.86, 86.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.50, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.146 CPU sec, 5.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13851, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 190, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (190.71, 95.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (235.37, 123.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.237 CPU sec, 5.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13382, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 153, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (164.31, 102.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (232.54, 121.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.534 CPU sec, 5.747 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13215, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 191, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (202.93, 103.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (231.33, 119.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.348 CPU sec, 7.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13069, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (160.59, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (228.84, 117.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.318 CPU sec, 7.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13360, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 163, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (200.35, 140.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.10, 125.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.385 CPU sec, 6.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13934, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 196, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (215.25, 102.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 212, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (245.94, 130.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.331 CPU sec, 6.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13948, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 180, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (231.16, 165.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (246.40, 131.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.137 CPU sec, 6.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13856, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 202, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (200.13, 113.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.79, 130.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.264 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14109, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (120.17, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (243.64, 129.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.310 CPU sec, 6.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13796, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 170, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (160.63, 96.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.58, 129.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.699 CPU sec, 5.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13884, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 156, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (181.24, 103.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (243.53, 128.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.253 CPU sec, 6.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13959, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (153.81, 99.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (245.91, 130.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.636 CPU sec, 6.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13714, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 104, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (161.57, 104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (244.81, 129.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.579 CPU sec, 7.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13957, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 184, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (152.20, 78.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.19, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.262 CPU sec, 6.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13953, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 178, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (184.61, 113.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 222, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (257.10, 137.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.723 CPU sec, 5.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13891, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 174, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (176.30, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (255.43, 135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.760 CPU sec, 6.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13975, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 151, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (165.92, 94.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (255.76, 136.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.498 CPU sec, 7.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13887, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 222, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (205.38, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (253.84, 135.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.870 CPU sec, 6.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14091, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (166.67, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (254.08, 134.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.494 CPU sec, 6.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13886, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 135, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (163.47, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (255.38, 135.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.200 CPU sec, 6.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14084, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 144, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (131.45, 54.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (254.93, 135.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.792 CPU sec, 6.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14040, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (142.72, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (254.11, 134.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.097 CPU sec, 6.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13991, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 224, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (218.73, 96.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (250.51, 131.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.848 CPU sec, 5.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13614, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 188, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (188.47, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (248.67, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.431 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13583, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 145, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (146.55, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (249.58, 131.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.085 CPU sec, 5.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13503, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 217, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (239.08, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (248.41, 131.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.591 CPU sec, 6.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13737, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 161, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (197.38, 146.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (253.33, 134.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.584 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13891, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 249, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (264.47, 146.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.71, 134.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.273 CPU sec, 7.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13797, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 213, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (213.89, 152.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (251.40, 133.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.001 CPU sec, 7.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13939, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 168, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (197.58, 118.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (255.64, 134.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.109 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13775, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 169, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (194.27, 120.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (251.98, 134.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.881 CPU sec, 6.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13999, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 176, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (187.78, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (255.07, 134.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.001 CPU sec, 5.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13876, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 157, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (178.62, 102.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (254.02, 135.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.052 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14006, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 185, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (178.32, 100.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.76, 133.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.767 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13899, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 203, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (200.85, 68.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (248.43, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.877 CPU sec, 5.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14016, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 242, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (249.33, 130.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (247.60, 131.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.674 CPU sec, 4.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13881, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (128.41, 75.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (244.60, 130.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.728 CPU sec, 6.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13781, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 159, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (184.80, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (246.55, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.383 CPU sec, 5.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13875, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 214, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (200.70, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (243.50, 128.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.459 CPU sec, 6.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13953, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 161, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (189.00, 121.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.21, 126.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.290 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13885, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 166, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (142.67, 63.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (242.39, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.718 CPU sec, 5.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13760, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 191, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (197.40, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (241.66, 127.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.217 CPU sec, 6.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13919, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 166, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (181.79, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.43, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.986 CPU sec, 7.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13988, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 149, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (157.45, 58.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (255.92, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.441 CPU sec, 6.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14050, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 138, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (155.00, 81.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (257.31, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.199 CPU sec, 7.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14044, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 121, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (156.64, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (255.21, 134.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.648 CPU sec, 6.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13876, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 214, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (233.88, 125.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (255.25, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.305 CPU sec, 6.609 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14019, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 194, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (186.26, 103.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (254.69, 135.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.916 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13896, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (223.71, 114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (255.44, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.412 CPU sec, 6.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14089, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (158.71, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 218, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (254.05, 135.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.205 CPU sec, 6.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13906, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 184, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (183.64, 117.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (254.04, 134.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.422 CPU sec, 7.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13984, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 162, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (169.32, 70.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (251.52, 135.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.568 CPU sec, 6.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13765, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 161, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (153.00, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (250.53, 132.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.214 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13415, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 150, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (168.25, 81.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (251.23, 133.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.195 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13603, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 132, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (141.84, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (253.11, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.489 CPU sec, 5.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13658, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 175, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (163.14, 71.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (250.13, 131.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.104 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13794, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 153, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (155.29, 70.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (251.16, 134.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.984 CPU sec, 5.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13733, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 154, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (169.60, 109.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (252.32, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.772 CPU sec, 7.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13817, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 181, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (189.23, 95.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.84, 134.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.665 CPU sec, 7.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13797, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 237, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (211.72, 101.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (251.10, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.946 CPU sec, 5.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13769, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 251, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (244.93, 166.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.27, 134.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.109 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13959, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 160, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (220.26, 152.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (255.39, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.513 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14025, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 136, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (136.29, 59.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.11, 134.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.589 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13732, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 174, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (181.08, 109.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (244.48, 129.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.834 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13925, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 180, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (198.00, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (243.46, 129.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.233 CPU sec, 6.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13940, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (180.96, 128.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.98, 128.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.311 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13856, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 122, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (140.75, 78.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (242.02, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.909 CPU sec, 6.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13897, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 206, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (201.83, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (239.99, 127.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.747 CPU sec, 5.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13953, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 164, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (161.55, 87.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.66, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.147 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13720, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 169, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (163.35, 95.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (238.70, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.934 CPU sec, 5.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13833, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 125, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (147.90, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (238.43, 125.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.612 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13893, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 195, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (217.68, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (250.52, 133.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.986 CPU sec, 5.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13932, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 176, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (212.60, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.93, 135.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.279 CPU sec, 4.963 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13839, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 165, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (168.89, 81.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (255.61, 134.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.142 CPU sec, 6.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13902, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 230, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (212.13, 108.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (255.72, 135.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.082 CPU sec, 6.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14072, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 167, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (183.44, 124.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (253.73, 135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.300 CPU sec, 5.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13886, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 159, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (159.40, 103.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (255.37, 135.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.654 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13944, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 172, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (216.95, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (249.05, 132.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.768 CPU sec, 5.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13889, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 177, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (209.62, 139.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (251.30, 134.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.140 CPU sec, 7.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13987, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 180, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (193.48, 101.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (249.03, 132.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.935 CPU sec, 6.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14086, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 188, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (202.00, 116.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (248.91, 131.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.282 CPU sec, 5.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13729, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 173, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (212.32, 105.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 216, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (250.29, 130.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.444 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13397, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 158, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (145.42, 67.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (249.65, 131.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.773 CPU sec, 6.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13667, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 167, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (192.80, 103.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (250.61, 131.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.274 CPU sec, 6.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13649, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 179, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (167.00, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (251.31, 132.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.298 CPU sec, 6.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13982, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 134, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (176.92, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (253.16, 135.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.799 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13856, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 212, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (184.19, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (253.95, 134.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.233 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13779, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 143, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (164.94, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (252.99, 133.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.539 CPU sec, 5.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13955, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 194, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (181.60, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (256.50, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.512 CPU sec, 5.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13983, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 166, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (161.62, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.04, 136.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.495 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13995, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (210.81, 72.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 218, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (254.10, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.961 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13983, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 112, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (150.10, 99.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (253.26, 134.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.009 CPU sec, 7.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13653, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 135, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (149.54, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (240.54, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.381 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13807, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 181, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (188.69, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (243.39, 128.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.452 CPU sec, 5.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13817, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 175, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (212.06, 132.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.88, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.997 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 14098, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 183, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (184.63, 101.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (243.22, 127.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.256 CPU sec, 6.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13980, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 127, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (109.73, 58.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (242.38, 126.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.055 CPU sec, 5.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13896, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 195, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (186.27, 108.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (240.98, 127.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.708 CPU sec, 4.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13817, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 176, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (177.23, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (244.16, 128.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.069 CPU sec, 6.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13688, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 153, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (154.68, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (240.58, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.447 CPU sec, 5.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14006, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 153, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (152.12, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (251.84, 134.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.722 CPU sec, 5.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13766, 9, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 199, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (197.83, 95.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.74, 133.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.373 CPU sec, 6.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13904, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 183, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (176.92, 99.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (252.72, 133.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.928 CPU sec, 6.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13946, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (222.91, 145.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.84, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.472 CPU sec, 7.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13772, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (131.37, 71.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (250.48, 133.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.448 CPU sec, 6.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14023, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 232, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (213.87, 113.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (250.12, 133.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.205 CPU sec, 7.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13835, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 208, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (185.22, 112.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (249.26, 133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.022 CPU sec, 7.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13798, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 154, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (172.21, 96.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (250.10, 132.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.901 CPU sec, 6.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13925, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 187, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (194.88, 101.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (247.62, 130.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.018 CPU sec, 6.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13900, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 130, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (183.33, 115.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (246.83, 130.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.512 CPU sec, 7.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13409, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 137, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (140.45, 75.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.37, 126.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.245 CPU sec, 6.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13559, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (130.47, 62.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (239.02, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.729 CPU sec, 6.683 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13574, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 169, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (162.19, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.26, 126.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.728 CPU sec, 6.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13712, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 108, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (131.39, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (242.54, 128.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.487 CPU sec, 6.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13724, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 176, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (175.89, 80.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (244.52, 129.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.907 CPU sec, 6.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13707, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 153, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (185.32, 142.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (246.29, 130.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.534 CPU sec, 6.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13986, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (171.40, 109.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (246.97, 132.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.082 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13662, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 153, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (151.06, 58.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (248.14, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.840 CPU sec, 6.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13868, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 147, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (138.48, 61.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (249.98, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.922 CPU sec, 6.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13892, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 143, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (180.16, 108.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (249.83, 132.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.761 CPU sec, 6.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13953, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 153, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (150.27, 69.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (243.46, 129.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.876 CPU sec, 6.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13839, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 184, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (200.41, 100.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (242.27, 127.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.691 CPU sec, 6.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 14218, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (173.93, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.76, 127.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.317 CPU sec, 5.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13906, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 143, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (166.31, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (242.76, 128.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.631 CPU sec, 5.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13839, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 156, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (171.94, 89.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.07, 125.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.266 CPU sec, 5.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13718, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 184, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (212.00, 113.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.80, 126.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.867 CPU sec, 6.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13848, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (158.84, 106.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (240.96, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.226 CPU sec, 7.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13908, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 153, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (156.85, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (242.47, 127.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.268 CPU sec, 6.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13761, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 234, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (203.95, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (241.58, 127.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.332 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13690, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 169, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (179.25, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (251.58, 134.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.986 CPU sec, 6.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14006, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 182, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (177.20, 102.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (249.95, 132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.539 CPU sec, 7.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14117, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 154, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (171.29, 112.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (250.62, 132.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.234 CPU sec, 6.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13958, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 145, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (172.73, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (252.48, 134.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.361 CPU sec, 6.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13951, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (206.89, 129.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (249.18, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.416 CPU sec, 5.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14028, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 215, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (218.29, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (250.49, 132.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.551 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13797, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 186, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (197.59, 110.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (249.57, 132.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.541 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13981, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 133, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (178.86, 130.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (246.19, 130.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.158 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14007, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 172, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (203.70, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (248.24, 131.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.434 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13961, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 180, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (175.94, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 212, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (246.15, 129.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.875 CPU sec, 6.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13334, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 147, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (155.05, 98.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (239.17, 125.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.209 CPU sec, 5.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13240, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 162, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (182.48, 88.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.32, 125.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.946 CPU sec, 6.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13583, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 166, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (171.00, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (241.56, 126.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.900 CPU sec, 6.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13424, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (206.13, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (243.41, 127.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.974 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13833, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 138, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (223.15, 215.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (248.19, 130.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.401 CPU sec, 6.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13969, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 179, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (192.61, 93.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 209, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (244.72, 129.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.436 CPU sec, 6.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13961, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 176, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (171.06, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (247.99, 130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.950 CPU sec, 6.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13927, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 141, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (162.47, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (250.89, 131.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.992 CPU sec, 6.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13919, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 207, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (195.58, 130.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (248.18, 133.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.041 CPU sec, 6.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13997, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 218, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (202.62, 114.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (250.31, 134.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.437 CPU sec, 5.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13976, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (176.63, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.24, 129.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.307 CPU sec, 6.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13798, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 121, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (132.36, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (241.82, 127.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.752 CPU sec, 6.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13727, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 190, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (211.16, 113.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (240.13, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.980 CPU sec, 6.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13809, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 143, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (193.96, 142.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (243.05, 128.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.164 CPU sec, 6.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13855, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 181, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (187.84, 92.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.70, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.975 CPU sec, 6.128 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13701, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (151.77, 93.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (239.01, 125.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.416 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13794, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 111, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (137.46, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.47, 124.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.546 CPU sec, 5.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13730, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (169.73, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (237.44, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.157 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13856, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 146, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (169.12, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (243.42, 127.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.604 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13790, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 179, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (226.24, 160.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 212, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (248.92, 133.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.177 CPU sec, 6.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13831, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 233, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (222.45, 112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (250.18, 133.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.437 CPU sec, 5.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13744, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 196, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (193.30, 92.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (247.42, 131.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.352 CPU sec, 6.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13987, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (250.05, 101.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (248.39, 131.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.862 CPU sec, 6.888 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13749, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (149.47, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (249.65, 133.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.139 CPU sec, 6.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13879, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 184, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (201.00, 123.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (248.64, 131.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.880 CPU sec, 6.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13885, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 170, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (177.10, 120.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.53, 130.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.394 CPU sec, 6.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13769, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 154, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (170.00, 83.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (245.91, 130.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.145 CPU sec, 5.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13732, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (160.96, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.58, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.250 CPU sec, 6.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13619, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 150, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (170.48, 103.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (243.19, 129.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.992 CPU sec, 5.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13586, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 208, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (198.95, 70.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (247.28, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.920 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13567, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (202.35, 121.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (243.80, 128.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.462 CPU sec, 6.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13865, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 176, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (164.11, 89.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (245.94, 130.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.091 CPU sec, 5.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13760, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 183, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (169.81, 78.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (244.72, 130.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.731 CPU sec, 5.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13899, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (158.72, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (247.45, 130.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.595 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13941, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 197, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (187.60, 117.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (247.89, 131.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.448 CPU sec, 7.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13867, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 150, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (144.42, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (247.54, 132.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.024 CPU sec, 6.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13981, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 184, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (180.00, 95.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 214, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (250.58, 134.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.041 CPU sec, 5.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13880, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (222.86, 89.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (250.00, 132.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.539 CPU sec, 5.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13906, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 219, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (189.59, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (249.65, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.822 CPU sec, 5.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13730, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 156, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (164.38, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (241.31, 127.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.695 CPU sec, 5.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13644, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 157, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (189.96, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (238.05, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.139 CPU sec, 5.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13659, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 192, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (196.25, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (238.85, 125.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.935 CPU sec, 7.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13668, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 155, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (150.52, 81.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (234.95, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.309 CPU sec, 4.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13468, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (155.50, 102.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (235.49, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.027 CPU sec, 6.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13450, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 125, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (156.96, 94.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (234.50, 120.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.143 CPU sec, 5.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13328, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 195, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (209.76, 113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (235.85, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.292 CPU sec, 5.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13070, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 159, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (145.77, 63.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.75, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.460 CPU sec, 7.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13288, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 162, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (176.17, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (247.56, 130.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.117 CPU sec, 6.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13981, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 188, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (199.63, 105.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (254.47, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.840 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13917, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 162, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (156.69, 107.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (253.27, 135.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.213 CPU sec, 6.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13913, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 193, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (171.38, 91.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.80, 134.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.416 CPU sec, 6.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14077, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 234, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (180.88, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (251.75, 134.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.197 CPU sec, 5.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13903, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 152, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (160.64, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.43, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.626 CPU sec, 6.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13901, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 139, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (171.37, 81.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (247.90, 133.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.804 CPU sec, 7.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13909, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 158, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (167.48, 95.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (250.98, 134.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.555 CPU sec, 6.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13830, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 178, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (196.21, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (249.60, 132.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.561 CPU sec, 6.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14007, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 207, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (219.59, 133.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (246.06, 132.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.059 CPU sec, 7.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13836, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 150, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (167.67, 103.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (249.78, 132.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.129 CPU sec, 6.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13752, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 191, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (211.00, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (255.02, 133.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.498 CPU sec, 5.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13953, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 171, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (206.37, 121.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (254.85, 134.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.099 CPU sec, 6.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14059, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 150, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (158.77, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (256.87, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.877 CPU sec, 5.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13754, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 174, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (238.00, 221.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (255.45, 135.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.484 CPU sec, 5.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13893, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 197, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (171.50, 118.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (254.15, 134.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.558 CPU sec, 7.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13977, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 164, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (143.94, 61.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (255.70, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.243 CPU sec, 6.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13752, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 181, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (185.13, 88.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 219, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (255.29, 135.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.078 CPU sec, 7.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13904, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (255.57, 135.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.255 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13910, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 222, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (208.50, 138.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (252.63, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.806 CPU sec, 6.883 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13957, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 151, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (178.13, 106.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.29, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.893 CPU sec, 5.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13865, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 263, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (226.61, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (244.58, 130.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.681 CPU sec, 6.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13725, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (169.90, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (242.56, 128.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.135 CPU sec, 6.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13586, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 138, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (144.47, 77.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (242.31, 126.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.576 CPU sec, 6.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13753, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 173, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (185.50, 116.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (239.22, 126.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.679 CPU sec, 5.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13414, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 170, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (150.68, 65.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (234.73, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.851 CPU sec, 6.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13273, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 138, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (149.00, 89.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (233.35, 120.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.871 CPU sec, 5.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12972, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 215, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (195.17, 103.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.54, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.479 CPU sec, 5.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13125, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 120, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (152.37, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.67, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.689 CPU sec, 6.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13582, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 144, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (165.22, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (250.91, 132.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.352 CPU sec, 6.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13895, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 258, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (231.61, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.61, 137.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.365 CPU sec, 6.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13994, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 181, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (184.72, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (256.18, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.519 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13958, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 162, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (188.88, 100.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (255.47, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.555 CPU sec, 6.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14021, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (159.05, 100.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (254.62, 135.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.829 CPU sec, 6.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14150, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 132, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (163.21, 98.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 217, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (254.01, 136.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.372 CPU sec, 6.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13936, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (170.91, 116.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (253.17, 134.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.756 CPU sec, 8.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13983, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 212, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (189.56, 109.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (251.66, 133.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.356 CPU sec, 6.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13971, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (143.26, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (252.00, 134.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.610 CPU sec, 5.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13929, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 208, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (198.42, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (252.38, 134.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.247 CPU sec, 5.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13791, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 192, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (182.59, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (252.44, 133.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.591 CPU sec, 5.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13814, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 140, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (170.89, 105.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (256.24, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.895 CPU sec, 5.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13761, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 164, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (154.91, 61.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 226, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (259.41, 136.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.000 CPU sec, 6.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13855, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 143, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (182.37, 106.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (257.74, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.622 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13819, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 151, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (167.74, 90.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 222, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (256.42, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.428 CPU sec, 6.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13877, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 175, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (209.65, 132.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 225, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (259.55, 138.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.809 CPU sec, 6.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13892, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 209, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (198.93, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 223, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (259.10, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.530 CPU sec, 6.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13812, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 161, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (181.12, 116.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (255.94, 135.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.298 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13949, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 188, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (178.05, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (256.53, 136.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.934 CPU sec, 6.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13943, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 306, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.77, 110.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (258.14, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.185 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13831, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 133, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (149.00, 92.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.57, 134.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.667 CPU sec, 7.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13908, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 158, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (186.77, 113.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (244.91, 130.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.903 CPU sec, 5.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13978, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 128, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (140.04, 85.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.49, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.134 CPU sec, 5.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13749, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 124, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 258) [M::mem_pestat] mean and std.dev: (120.32, 31.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (245.14, 129.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.099 CPU sec, 7.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13679, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (144.92, 92.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.49, 127.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.346 CPU sec, 5.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13491, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (148.48, 100.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.30, 125.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.920 CPU sec, 5.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13562, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 172, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 305) [M::mem_pestat] mean and std.dev: (164.86, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.64, 123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.957 CPU sec, 6.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13368, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 185, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (203.09, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.16, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.380 CPU sec, 5.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13181, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (162.00, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (237.98, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.354 CPU sec, 5.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13395, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 140, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (175.20, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (246.14, 129.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.969 CPU sec, 6.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 14032, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 138, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (125.00, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (255.44, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.858 CPU sec, 7.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13950, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 144, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (163.89, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (255.62, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.134 CPU sec, 5.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13945, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 153, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (178.61, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 219, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (254.22, 134.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.242 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14093, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 186, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (203.68, 94.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (252.02, 135.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.330 CPU sec, 5.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13986, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 141, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (148.57, 103.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (254.78, 135.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.053 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14023, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 148, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (215.67, 130.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (252.86, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.266 CPU sec, 6.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13853, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 160, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (185.77, 115.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.23, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.100 CPU sec, 6.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13943, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 183, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (157.09, 94.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (253.72, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.343 CPU sec, 6.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14144, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 140, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (184.68, 144.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (253.72, 134.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.701 CPU sec, 5.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14000, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 177, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (198.11, 107.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (251.23, 133.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.429 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14029, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 223, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (223.54, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 223, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (257.21, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.217 CPU sec, 6.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13924, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 162, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (166.04, 87.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 223, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (257.05, 136.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.206 CPU sec, 5.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13980, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 184, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (218.04, 169.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (256.01, 136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.591 CPU sec, 6.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13901, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 146, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (171.93, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (256.21, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.467 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14094, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 172, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (167.53, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 220, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (256.62, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.930 CPU sec, 6.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14175, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 137, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (180.76, 111.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (254.79, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.833 CPU sec, 5.435 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14102, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 98, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (92.00, 48.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (254.15, 134.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.973 CPU sec, 5.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14081, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 173, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (206.67, 95.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 221, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (255.58, 134.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.944 CPU sec, 6.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 14129, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (167.08, 96.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (255.57, 136.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.220 CPU sec, 6.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13868, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (128.11, 62.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 210, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (246.16, 131.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.624 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13722, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 160, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (204.36, 100.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (237.80, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.677 CPU sec, 6.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13759, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 178, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (171.86, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.81, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.206 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13682, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 134, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (157.10, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (235.60, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.884 CPU sec, 7.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13493, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 137, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (154.83, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (233.64, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.043 CPU sec, 9.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13494, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (193.95, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.24, 120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.678 CPU sec, 6.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13268, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 161, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (167.55, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (227.97, 117.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.590 CPU sec, 5.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13101, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 137, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (155.75, 76.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (225.98, 116.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.632 CPU sec, 6.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12922, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 167, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (168.32, 82.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (225.84, 115.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.797 CPU sec, 6.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13821, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 146, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (135.42, 51.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (244.44, 129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.278 CPU sec, 6.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13952, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 185, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (182.53, 93.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (249.69, 131.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.280 CPU sec, 7.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13800, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 216, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (203.17, 120.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (250.43, 132.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.539 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13795, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 178, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (199.05, 95.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (248.30, 132.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.971 CPU sec, 5.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13878, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 176, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (181.61, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.88, 131.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.398 CPU sec, 6.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13856, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 161, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (149.12, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (246.31, 130.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.104 CPU sec, 6.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13788, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (146.17, 102.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (246.91, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.059 CPU sec, 5.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13946, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 153, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (180.23, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (247.63, 131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.520 CPU sec, 6.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13918, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 215, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (213.08, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (244.96, 130.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.424 CPU sec, 6.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13947, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 191, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (194.45, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.80, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.595 CPU sec, 6.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13699, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 111, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (109.40, 50.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (252.37, 132.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.577 CPU sec, 6.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13813, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 178, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (178.23, 81.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.93, 133.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.370 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13806, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 148, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (166.12, 90.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (251.36, 134.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.462 CPU sec, 7.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13734, 9, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 148, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (172.37, 116.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (248.77, 132.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.686 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13840, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (149.24, 84.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (251.14, 133.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.191 CPU sec, 6.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13889, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 150, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (163.00, 100.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.63, 134.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.501 CPU sec, 6.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13677, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 136, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (145.19, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (252.34, 134.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.296 CPU sec, 6.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13736, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 147, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (150.60, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (252.24, 133.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.257 CPU sec, 6.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13839, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 126, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (128.21, 63.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (252.28, 132.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.467 CPU sec, 6.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13954, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 168, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (162.27, 99.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.90, 133.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.124 CPU sec, 6.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13940, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 152, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (160.87, 90.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (241.03, 127.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.683 CPU sec, 5.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13897, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 138, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (182.70, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (235.36, 123.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.655 CPU sec, 6.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13729, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 146, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (142.40, 63.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (235.63, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.935 CPU sec, 6.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13833, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 127, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (119.77, 66.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (231.56, 120.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.952 CPU sec, 6.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13472, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (190.85, 158.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (233.16, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.628 CPU sec, 5.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13476, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 168, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (200.35, 134.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 194, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (225.42, 115.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.816 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13244, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (180.92, 103.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (225.45, 115.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.536 CPU sec, 6.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12815, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 132, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (167.04, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 193, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (225.33, 115.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.100 CPU sec, 7.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13152, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (160.83, 99.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (236.80, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.959 CPU sec, 7.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13975, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 155, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (154.78, 100.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (252.87, 133.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.746 CPU sec, 7.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14010, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 220, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (242.31, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (251.00, 134.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.546 CPU sec, 6.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13993, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 190, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (191.38, 114.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (250.19, 133.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.312 CPU sec, 7.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13985, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 141, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (175.42, 130.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (248.71, 132.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.757 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13807, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 181, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (183.80, 110.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (248.01, 132.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.091 CPU sec, 6.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13890, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 159, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (190.89, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (249.23, 132.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.507 CPU sec, 6.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13935, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 198, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (180.00, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (246.61, 130.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.601 CPU sec, 6.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13904, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 169, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (178.68, 106.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (247.96, 131.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.107 CPU sec, 5.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14050, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 107, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (159.33, 94.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (244.35, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.354 CPU sec, 8.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13808, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 188, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (178.42, 107.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (246.21, 130.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.430 CPU sec, 6.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13967, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 150, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (155.37, 81.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (250.57, 131.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.809 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13914, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (167.18, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (252.88, 134.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.659 CPU sec, 7.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13812, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 140, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (140.10, 56.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (251.38, 135.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.172 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13843, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 119, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (159.67, 85.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.54, 133.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.815 CPU sec, 6.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13703, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 177, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (224.67, 133.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 213, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (249.70, 133.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.000 CPU sec, 6.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 14015, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 218, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (254.32, 134.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 151.541 CPU sec, 6.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13816, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 183, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (184.63, 96.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (253.60, 134.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.125 CPU sec, 6.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13981, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 183, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (183.35, 100.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (252.20, 133.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.618 CPU sec, 6.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13827, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 187, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (202.90, 139.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (255.83, 135.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.469 CPU sec, 6.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13895, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 164, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (163.18, 92.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (249.28, 133.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.091 CPU sec, 5.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13976, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 125, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (138.38, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (242.86, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.778 CPU sec, 6.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14033, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 209, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (210.55, 123.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (239.21, 125.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.077 CPU sec, 5.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13820, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (126.04, 67.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (239.46, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.739 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13682, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 129, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (134.04, 72.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.87, 124.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.852 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13645, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 163, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (171.88, 84.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (235.97, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.110 CPU sec, 5.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13609, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 213, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (209.87, 91.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (232.23, 120.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.562 CPU sec, 6.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13282, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 155, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (167.08, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (231.59, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.213 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13101, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (149.87, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.92, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.413 CPU sec, 7.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13696, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (131.55, 69.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.73, 129.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.854 CPU sec, 5.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13962, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 165, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (193.62, 89.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (250.93, 133.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.061 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14018, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 165, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (157.83, 89.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 215, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (252.11, 134.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.302 CPU sec, 6.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13985, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 103, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (137.83, 84.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (253.75, 136.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.634 CPU sec, 6.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13953, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 187, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (204.64, 107.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 214, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (249.02, 132.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.856 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13847, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 180, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (159.36, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (249.48, 133.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.223 CPU sec, 5.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13850, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 152, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (191.53, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (247.09, 132.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.605 CPU sec, 6.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13999, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 280, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (251.07, 178.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 211, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (248.90, 133.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.604 CPU sec, 5.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13965, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (152.67, 80.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (248.11, 131.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.284 CPU sec, 6.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13884, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 145, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (161.83, 103.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.87, 131.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.700 CPU sec, 5.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13817, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 97, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (145.22, 105.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (249.22, 130.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.171 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13823, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 193, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (222.08, 135.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (251.70, 132.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.970 CPU sec, 6.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13862, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 208, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (209.45, 112.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (249.98, 132.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.746 CPU sec, 6.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13916, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (178.47, 77.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 219, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (254.90, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.459 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13912, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (173.45, 106.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 217, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (252.25, 134.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.880 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13879, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 182, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (164.78, 86.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 213, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (249.38, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.448 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13932, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 204, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (190.69, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 215, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (251.13, 132.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.077 CPU sec, 6.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13987, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 196, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (197.56, 95.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (251.42, 133.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.447 CPU sec, 6.085 real sec [M::process] read 52386 sequences (7910286 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 14032, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 161, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (158.42, 90.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 217, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (252.43, 133.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.880 CPU sec, 6.582 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11154, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 173, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (230.55, 136.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (250.06, 132.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 52386 reads in 124.241 CPU sec, 6.045 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21183\tSM:UG10S2P518\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21183_HLKNYBGXX_UG10S2P518_R1.fastq.gz 5347_7332_21183_HLKNYBGXX_UG10S2P518_R2.fastq.gz [main] Real time: 4413.422 sec; CPU: 106563.642 sec Finished alignement of UG10S2P518 at Wed Apr 3 18:23:22 2019 Sorting BAM file started at Wed Apr 3 18:23:22 2019 samtools sort -@ 30 -m 4G UG10S2P518_unsorted.bam -o UG10S2P518.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 18:23:22 2019 rm UG10S2P518_unsorted.bam rm 5347_7332_21183_HLKNYBGXX_UG10S2P518_R1.fastq.gz rm 5347_7332_21183_HLKNYBGXX_UG10S2P518_R2.fastq.gz samtools index UG10S2P518.bam Finished BAM file generation of the sample - UG10S2P518 Processing fastq files for the sample - UG10S2P520 Reading 5347_7332_21184_HLKNYBGXX_UG10S2P520_R1.fastq.gz 5347_7332_21184_HLKNYBGXX_UG10S2P520_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21184\tSM:UG10S2P520\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21184_HLKNYBGXX_UG10S2P520_R1.fastq.gz 5347_7332_21184_HLKNYBGXX_UG10S2P520_R2.fastq.gz | samtools view -Sb1 - > UG10S2P520_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12064, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 218, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (229.00, 130.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.89, 107.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.689 CPU sec, 6.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12187, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 104, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (120.06, 64.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.08, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.299 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12358, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (114.56, 44.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.23, 109.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.816 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12347, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 139, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (170.19, 143.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (227.79, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.277 CPU sec, 5.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12450, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 174, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (179.14, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (228.71, 109.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.510 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12423, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 124, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (127.69, 78.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.74, 108.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.104 CPU sec, 7.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12503, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 110, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (130.59, 78.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (224.95, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.258 CPU sec, 5.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12370, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 83, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (136.38, 105.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.70, 108.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.847 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12556, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (208.20, 129.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (224.24, 108.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.349 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12417, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 227, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (251.25, 111.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.76, 107.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.714 CPU sec, 6.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12375, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 181, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (198.36, 108.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (224.79, 106.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.692 CPU sec, 4.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12517, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (110.67, 33.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.15, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.451 CPU sec, 4.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12252, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 259, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (247.67, 187.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (222.04, 106.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.433 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12705, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (222.25, 238.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (223.40, 106.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.171 CPU sec, 6.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12612, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 134, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (158.58, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (220.78, 106.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.122 CPU sec, 5.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12441, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 114, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (130.47, 90.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.50, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.239 CPU sec, 4.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11966, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 89, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (122.06, 91.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.47, 106.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.027 CPU sec, 5.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12080, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 161, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (156.19, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.09, 106.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.748 CPU sec, 5.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12227, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 151, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (168.27, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (223.11, 106.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.641 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12285, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 182, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (156.64, 72.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (226.80, 110.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.335 CPU sec, 5.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12341, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 143, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (138.87, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.77, 109.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.275 CPU sec, 4.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12318, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 115, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (179.72, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (226.05, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.446 CPU sec, 6.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12479, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 131, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (165.43, 124.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.36, 108.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.321 CPU sec, 5.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12520, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (101.77, 55.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.59, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.317 CPU sec, 4.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12453, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (157.48, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (226.78, 109.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.787 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12639, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 129, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (140.35, 68.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (226.06, 108.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.036 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12575, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 115, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (142.29, 72.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (224.89, 109.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.864 CPU sec, 5.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12314, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 153, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (166.30, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (224.64, 106.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.917 CPU sec, 5.529 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12483, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (153.13, 75.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (221.33, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.259 CPU sec, 5.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12490, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 216, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (187.42, 110.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (221.99, 106.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.831 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12483, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 145, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (160.10, 93.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.34, 105.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.619 CPU sec, 5.377 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12457, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 136, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (146.50, 98.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (220.23, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.617 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12141, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 133, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (117.43, 49.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.06, 107.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.636 CPU sec, 6.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12088, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (126.12, 31.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (221.70, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.904 CPU sec, 5.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12142, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 127, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (117.65, 48.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (224.65, 107.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.803 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12259, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 209, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (188.42, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.81, 107.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.804 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12387, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (188.65, 96.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.59, 109.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.611 CPU sec, 5.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12352, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (168.30, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (223.68, 106.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.639 CPU sec, 6.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12295, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 85, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (138.82, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (224.04, 107.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.482 CPU sec, 6.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12467, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 129, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (137.90, 63.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (227.47, 109.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.345 CPU sec, 5.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12287, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 133, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (150.52, 78.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (222.69, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.849 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12417, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 144, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (173.33, 81.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (222.98, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.664 CPU sec, 6.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12486, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (169.19, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.66, 107.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.651 CPU sec, 5.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12379, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 137, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (147.38, 77.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.85, 107.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.641 CPU sec, 6.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12660, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 133, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (138.29, 83.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.59, 106.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.487 CPU sec, 5.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12442, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (207.12, 132.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.89, 106.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.337 CPU sec, 6.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12403, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (131.20, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.16, 105.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.229 CPU sec, 6.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12248, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 144, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (159.93, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (221.34, 103.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.540 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12103, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 76, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (108.47, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (221.47, 105.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.429 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12276, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 141, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (152.14, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.03, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.831 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12337, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 141, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (161.64, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.44, 106.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.883 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12372, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 146, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (174.78, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.32, 107.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.330 CPU sec, 5.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12393, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 129, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (161.45, 100.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.19, 108.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.692 CPU sec, 5.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12491, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 124, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (113.69, 42.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (226.34, 108.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.064 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12383, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (160.22, 118.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.90, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.202 CPU sec, 5.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12484, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 206, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (205.00, 111.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.61, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.418 CPU sec, 6.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12596, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 125, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (141.26, 77.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.05, 106.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.159 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12676, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 151, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (151.65, 74.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (222.85, 105.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.838 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12549, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 201, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (211.82, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.48, 107.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.390 CPU sec, 5.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12527, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 136, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (147.06, 58.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.16, 104.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.474 CPU sec, 5.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12521, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (157.42, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (218.73, 103.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.891 CPU sec, 5.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12660, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 138, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (154.23, 96.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (222.53, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.158 CPU sec, 5.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12620, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 147, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (147.07, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (220.30, 104.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.082 CPU sec, 5.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12439, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (118.36, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (219.31, 102.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.662 CPU sec, 5.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12347, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 106, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (110.11, 54.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (219.96, 103.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.266 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12149, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 94, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (117.46, 62.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (218.35, 103.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.019 CPU sec, 5.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12172, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 162, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (161.53, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.55, 104.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.662 CPU sec, 5.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12299, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 133, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (160.69, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (221.96, 105.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.755 CPU sec, 6.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12402, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (122.08, 71.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.81, 105.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.836 CPU sec, 6.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12471, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 179, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (170.86, 90.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (225.24, 108.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.132 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12480, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 131, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (177.54, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (222.59, 106.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.515 CPU sec, 5.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12481, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 131, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (150.00, 70.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.33, 107.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.362 CPU sec, 5.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12461, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 268, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (188.90, 132.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (224.91, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.953 CPU sec, 5.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12432, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 155, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (162.87, 71.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (223.56, 106.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.087 CPU sec, 4.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12338, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 182, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (177.78, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.85, 106.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.134 CPU sec, 4.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12366, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 195, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (174.33, 60.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.56, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.871 CPU sec, 6.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12292, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 169, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (161.65, 67.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (218.23, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.519 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12353, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 156, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (186.08, 131.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (219.78, 103.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.889 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12464, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (133.13, 67.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (219.99, 103.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.728 CPU sec, 5.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12398, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 224, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (263.69, 169.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.27, 104.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.108 CPU sec, 5.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12572, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (136.11, 96.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (220.75, 104.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.343 CPU sec, 5.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12063, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 102, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (141.86, 101.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (220.19, 104.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.241 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12198, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (167.40, 62.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (220.35, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.622 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12123, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 127, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (179.21, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (219.59, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.180 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12285, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 135, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (150.57, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.38, 105.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.752 CPU sec, 6.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12459, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 180, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (172.36, 82.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (222.81, 106.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.381 CPU sec, 6.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12355, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 144, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (153.18, 77.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (225.13, 108.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.616 CPU sec, 5.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12430, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 82, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (102.62, 62.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.08, 107.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.521 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12352, 3, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 95, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (149.37, 116.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.07, 106.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.404 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12585, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 128, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (154.88, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.40, 105.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.848 CPU sec, 5.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12329, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 174, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (169.53, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (222.20, 105.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.309 CPU sec, 5.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12300, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 174, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (158.08, 87.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (222.48, 105.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.424 CPU sec, 5.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12347, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 155, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (153.08, 96.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.79, 105.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.691 CPU sec, 6.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12334, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (121.50, 68.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (220.65, 104.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.070 CPU sec, 5.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12467, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 168, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (159.91, 77.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (220.27, 104.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.151 CPU sec, 5.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12316, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 129, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (125.83, 30.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (217.59, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.527 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12396, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 153, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (160.73, 61.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (219.53, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.725 CPU sec, 5.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12219, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 176, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (156.58, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (226.35, 109.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.735 CPU sec, 5.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12395, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 135, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (148.07, 77.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (227.61, 109.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.886 CPU sec, 5.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12453, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 138, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (164.94, 112.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (225.87, 107.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.434 CPU sec, 4.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12283, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 129, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (137.73, 82.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (226.33, 108.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.879 CPU sec, 5.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12267, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 147, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 300) [M::mem_pestat] mean and std.dev: (140.75, 59.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.46, 107.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.112 CPU sec, 5.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12466, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 184, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (182.16, 107.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (227.76, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.936 CPU sec, 5.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12414, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 168, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (128.56, 75.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.96, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.756 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12391, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 162, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (166.33, 81.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.34, 107.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.931 CPU sec, 4.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12337, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 206, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (210.38, 79.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (224.99, 107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.603 CPU sec, 5.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12393, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 190, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (183.86, 112.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (223.34, 106.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.158 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12327, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 116, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (148.60, 102.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (223.74, 106.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.586 CPU sec, 5.828 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12597, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 114, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (137.95, 75.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.28, 104.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.467 CPU sec, 6.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12356, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 153, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (140.21, 59.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (220.79, 104.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.019 CPU sec, 6.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12320, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 155, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (158.20, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.27, 105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.560 CPU sec, 6.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12069, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 140, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (147.41, 85.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (218.17, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.378 CPU sec, 5.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11757, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 132, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (159.18, 91.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (217.52, 102.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.796 CPU sec, 6.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12089, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 133, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (221.88, 208.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (226.51, 108.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.820 CPU sec, 6.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12297, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (166.00, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 201, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (230.73, 111.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.559 CPU sec, 5.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12358, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 235, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (209.47, 93.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 201, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (229.28, 110.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.937 CPU sec, 5.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12414, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 144, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (136.95, 73.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (228.45, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.284 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12382, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 154, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (193.06, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (227.89, 111.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.574 CPU sec, 5.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12444, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (147.04, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (226.77, 108.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.910 CPU sec, 5.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12263, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (144.76, 81.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (227.35, 109.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.075 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12650, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (161.80, 83.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.02, 108.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.965 CPU sec, 5.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 12532, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (227.63, 109.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 148.177 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12248, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 147, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (175.08, 108.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.15, 108.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.530 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12439, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 133, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (157.10, 79.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (225.18, 107.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.301 CPU sec, 6.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12512, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 140, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (155.41, 63.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (223.99, 106.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.752 CPU sec, 4.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12367, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 128, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (160.90, 109.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (219.51, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.767 CPU sec, 5.136 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12175, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 193, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (182.00, 122.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (216.81, 102.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.599 CPU sec, 4.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12093, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 122, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (102.94, 57.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 191, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (217.51, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.250 CPU sec, 5.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11751, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 145, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (162.93, 116.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (216.18, 100.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.363 CPU sec, 5.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11754, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 101, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (123.86, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.91, 106.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.089 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12247, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (143.37, 91.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (228.41, 110.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.142 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12502, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 192, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (181.64, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (226.67, 108.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.849 CPU sec, 5.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12360, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 133, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (157.50, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (228.08, 109.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.858 CPU sec, 4.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12347, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (172.13, 109.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (227.80, 110.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.210 CPU sec, 4.062 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12469, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 121, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (135.80, 83.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (227.99, 109.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.753 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12283, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 153, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (173.56, 117.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.63, 108.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.202 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12329, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (190.05, 102.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (224.82, 107.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.904 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12372, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 114, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (148.11, 83.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (224.79, 108.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.012 CPU sec, 4.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12429, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 118, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (167.55, 115.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.77, 107.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.384 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12513, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 151, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (163.77, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.12, 107.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.873 CPU sec, 5.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12498, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 123, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (135.53, 86.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.30, 106.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.370 CPU sec, 3.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12543, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 199, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (182.64, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.04, 105.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.909 CPU sec, 4.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12275, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 127, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (148.96, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (219.83, 103.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.078 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11969, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 141, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (162.20, 109.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (215.90, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.062 CPU sec, 5.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11971, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 113, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (148.68, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (215.24, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.646 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11871, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 99, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (141.50, 81.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (218.79, 103.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.009 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12489, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 132, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (151.74, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.87, 106.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.092 CPU sec, 5.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12377, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (145.00, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (224.89, 107.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.452 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12206, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 157, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (269.53, 217.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.39, 107.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.721 CPU sec, 5.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12373, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 118, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (109.25, 50.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (224.60, 108.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.828 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12242, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 137, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (143.90, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (225.07, 107.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.026 CPU sec, 5.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12333, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 157, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (181.56, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.69, 107.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.385 CPU sec, 6.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12256, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 105, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (124.67, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.68, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.802 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12243, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 148, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (141.73, 68.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.14, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.263 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12598, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 121, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (126.89, 69.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.05, 109.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.924 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12288, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 129, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (156.38, 93.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (223.21, 106.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.187 CPU sec, 5.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12470, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 84, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (101.06, 55.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (221.00, 106.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.957 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12309, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 179, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (175.24, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (220.53, 103.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.632 CPU sec, 5.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12320, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 127, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (151.61, 64.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (217.35, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.149 CPU sec, 5.521 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12263, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 91, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (130.52, 95.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (217.76, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.899 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12161, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (131.08, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 189, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (214.31, 100.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.470 CPU sec, 6.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11732, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 149, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (144.50, 62.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (216.10, 101.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.357 CPU sec, 4.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12384, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 166, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (160.24, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (226.63, 109.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.835 CPU sec, 6.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12430, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 158, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (154.79, 94.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (226.98, 108.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.880 CPU sec, 5.522 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12417, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 108, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (142.15, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (226.67, 109.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.485 CPU sec, 5.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12297, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 126, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (139.12, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.87, 108.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.917 CPU sec, 5.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12464, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 113, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (143.87, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (226.46, 108.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.031 CPU sec, 4.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12331, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (149.11, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (226.67, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.583 CPU sec, 5.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12443, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 106, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (120.00, 63.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.37, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.885 CPU sec, 5.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12490, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 104, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (106.73, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (223.60, 107.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.887 CPU sec, 5.626 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12464, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 83, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (118.21, 104.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (226.03, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.295 CPU sec, 6.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12546, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (221.91, 105.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 134.722 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12340, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 101, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (124.21, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (221.96, 105.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.620 CPU sec, 5.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12370, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 126, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (145.89, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (218.70, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.013 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12456, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 100, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (118.73, 64.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (219.28, 103.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.448 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12233, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 126, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (134.48, 74.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 189, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (215.50, 101.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.541 CPU sec, 4.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11892, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 88, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (100.71, 39.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (214.98, 100.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.369 CPU sec, 5.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11641, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 189, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (195.50, 87.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (217.00, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.594 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12361, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 158, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (145.40, 79.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (227.18, 109.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.060 CPU sec, 5.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12175, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 151, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (162.64, 116.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (226.39, 109.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.363 CPU sec, 5.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12313, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 190, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (217.31, 160.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (224.95, 108.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.088 CPU sec, 5.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12318, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 156, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (139.00, 70.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (224.96, 107.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.881 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12363, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 117, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (142.62, 77.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.44, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.561 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12389, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (129.72, 59.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (225.21, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.506 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12280, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (164.00, 102.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.49, 108.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.373 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12455, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 124, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (143.30, 76.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (223.92, 106.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.754 CPU sec, 5.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12468, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 135, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (147.53, 112.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.00, 107.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.570 CPU sec, 5.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12485, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (142.60, 74.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.19, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.632 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12364, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 195, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (184.75, 99.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (220.89, 104.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.572 CPU sec, 5.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12257, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (165.53, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (220.82, 104.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.373 CPU sec, 7.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12322, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 73, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (117.62, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (217.97, 102.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.192 CPU sec, 6.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12119, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (150.64, 91.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (216.98, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.997 CPU sec, 5.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 11866, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 156, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (158.61, 83.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 188, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (212.62, 98.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.737 CPU sec, 4.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11720, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (156.00, 103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 190, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (214.01, 99.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.032 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12459, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 137, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (173.68, 133.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.35, 106.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.228 CPU sec, 4.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12442, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 155, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (177.11, 113.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.37, 105.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.001 CPU sec, 5.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12331, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (152.30, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (219.12, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.526 CPU sec, 4.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12346, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (155.81, 103.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (219.64, 103.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.398 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12424, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 158, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (199.14, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (221.35, 104.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.925 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12499, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 163, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (154.74, 67.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.30, 106.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.659 CPU sec, 6.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12388, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (124.71, 58.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 199, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (226.31, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.443 CPU sec, 7.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12359, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (131.67, 49.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (224.20, 107.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.506 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12501, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 140, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (143.91, 67.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (226.25, 107.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.281 CPU sec, 5.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12512, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 97, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (141.64, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (222.09, 105.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.217 CPU sec, 7.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 11907, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 95, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (114.56, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (223.92, 106.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.941 CPU sec, 5.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12263, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (126.67, 74.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.08, 106.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.280 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12249, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 125, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (158.41, 92.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (222.57, 105.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.983 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12299, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 138, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (122.00, 64.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (222.05, 106.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.193 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12537, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 133, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (147.78, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.87, 109.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.469 CPU sec, 6.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12534, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 139, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (137.08, 104.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (222.60, 105.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.395 CPU sec, 6.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12530, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 118, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (125.31, 44.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.48, 106.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.059 CPU sec, 4.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12394, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 117, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (134.95, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.37, 103.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.187 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12341, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (139.70, 72.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.17, 103.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.422 CPU sec, 4.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12373, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 159, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (165.67, 81.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (221.93, 105.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.399 CPU sec, 4.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12258, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (137.00, 64.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (224.56, 106.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.515 CPU sec, 4.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12325, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 138, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (143.00, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (227.67, 109.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.162 CPU sec, 5.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12578, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 107, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (122.74, 76.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (225.75, 107.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.993 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12345, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 184, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (170.06, 98.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (224.99, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.739 CPU sec, 5.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12426, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.39, 108.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.197 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12278, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (126.44, 75.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.87, 106.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.499 CPU sec, 5.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11958, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (118.90, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.78, 106.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.481 CPU sec, 4.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12196, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (131.06, 60.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.05, 105.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.880 CPU sec, 4.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12433, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (140.07, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (223.46, 106.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.998 CPU sec, 4.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12352, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (129.74, 81.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (223.11, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.297 CPU sec, 5.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12360, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 143, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (138.54, 77.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.33, 107.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.510 CPU sec, 5.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12472, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 139, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (123.91, 71.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (220.98, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.197 CPU sec, 5.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12588, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 148, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (177.68, 128.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (221.03, 103.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.744 CPU sec, 4.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12605, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 133, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (218.07, 219.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.04, 103.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.519 CPU sec, 5.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12518, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (137.60, 92.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (219.02, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.015 CPU sec, 5.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12632, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 128, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (161.55, 83.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (218.91, 102.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.940 CPU sec, 4.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12483, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 139, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (170.19, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (226.69, 108.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.743 CPU sec, 4.649 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12421, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 147, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (167.53, 95.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (226.75, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.670 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12430, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 149, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (158.21, 115.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (226.37, 109.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.561 CPU sec, 5.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12569, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 110, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (157.12, 102.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.64, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.088 CPU sec, 6.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12630, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 143, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (152.89, 99.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (224.42, 107.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.976 CPU sec, 5.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12076, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 116, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (151.27, 80.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (221.66, 104.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.613 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12162, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 98, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 192) [M::mem_pestat] mean and std.dev: (98.21, 28.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.82, 106.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.101 CPU sec, 5.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12267, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 176, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (190.55, 107.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (224.23, 108.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.410 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12486, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 149, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (172.14, 113.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.62, 107.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.820 CPU sec, 5.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12525, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 157, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (141.00, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 199, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (225.23, 107.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.304 CPU sec, 5.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12519, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (142.53, 83.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (224.43, 107.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.844 CPU sec, 5.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12300, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 151, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (152.27, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (217.96, 101.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.494 CPU sec, 5.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12472, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (132.60, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (218.81, 103.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.995 CPU sec, 5.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12385, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 206, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (204.87, 109.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (220.81, 103.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.923 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12412, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 237, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (182.00, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (220.09, 103.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.340 CPU sec, 6.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12314, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 163, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (177.73, 95.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (219.48, 104.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.171 CPU sec, 5.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12577, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 151, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (134.12, 50.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (221.41, 105.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.120 CPU sec, 5.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12430, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 196, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (186.45, 51.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (222.14, 106.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.518 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12440, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (182.20, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (221.82, 105.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.496 CPU sec, 4.332 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12273, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 135, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (191.05, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.59, 105.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.031 CPU sec, 4.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12262, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 164, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (174.15, 79.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.62, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.739 CPU sec, 5.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11871, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 174, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (154.94, 54.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (216.62, 100.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.705 CPU sec, 5.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12188, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 141, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (137.19, 75.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (217.83, 102.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.634 CPU sec, 5.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12243, 3, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 111, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (110.36, 44.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (220.30, 104.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.029 CPU sec, 4.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12319, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 156, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (155.84, 83.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (220.84, 104.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.995 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12394, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (114.08, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (222.12, 105.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.211 CPU sec, 5.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12605, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 181, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (189.94, 99.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.20, 108.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.668 CPU sec, 5.004 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12567, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 160, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (182.23, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.81, 104.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.668 CPU sec, 5.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12394, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 121, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (142.16, 71.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.09, 103.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.994 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12376, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (138.33, 70.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (219.57, 102.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.299 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12451, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 116, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (96.23, 43.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.24, 104.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.119 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12410, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 143, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (160.00, 94.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (222.76, 106.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.781 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12485, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 132, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (148.52, 98.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.46, 106.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.400 CPU sec, 5.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12684, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 113, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.12, 67.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (222.99, 106.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.233 CPU sec, 5.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12506, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 191, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (177.38, 85.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (223.62, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.359 CPU sec, 5.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12553, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (169.18, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (221.69, 106.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.451 CPU sec, 4.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12283, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (160.53, 98.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (219.66, 104.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.229 CPU sec, 5.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 11965, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 71, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (114.46, 88.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (216.99, 101.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.173 CPU sec, 5.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12392, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 191, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (167.18, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (218.29, 102.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.049 CPU sec, 4.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12229, 10, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 231, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (198.67, 104.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (219.04, 103.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 473, 2725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8145) [M::mem_pestat] mean and std.dev: (1358.56, 1991.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10855) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 126.170 CPU sec, 6.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12510, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 105, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (114.93, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.29, 104.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.629 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12516, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 124, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (131.05, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.49, 105.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.184 CPU sec, 4.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12394, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (202.19, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (219.34, 102.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.106 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12294, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 151, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (173.63, 100.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (219.39, 102.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.288 CPU sec, 4.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12377, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 136, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (156.24, 81.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (218.51, 101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.905 CPU sec, 5.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12252, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 149, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (163.14, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (217.87, 101.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.436 CPU sec, 5.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12432, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 135, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (167.89, 113.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (215.00, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.417 CPU sec, 5.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12417, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 217, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (208.11, 95.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.04, 106.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.790 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12463, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 140, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (140.11, 78.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.66, 106.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.918 CPU sec, 5.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12466, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (177.40, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.85, 104.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.721 CPU sec, 4.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12436, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (128.58, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (221.42, 106.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.095 CPU sec, 5.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12252, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 164, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (132.90, 66.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.35, 105.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.559 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12187, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 175, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (173.62, 99.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (219.39, 103.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.688 CPU sec, 5.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11924, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 145, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (128.50, 41.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (216.30, 100.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.527 CPU sec, 5.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12310, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 198, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 406) [M::mem_pestat] mean and std.dev: (169.80, 58.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (219.78, 105.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.498 CPU sec, 6.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12311, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (211.00, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (221.83, 106.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.570 CPU sec, 5.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12491, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 182, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (237.50, 133.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (219.74, 105.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.721 CPU sec, 5.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12451, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 118, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (156.44, 110.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (221.36, 105.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.162 CPU sec, 5.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12269, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 151, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 308) [M::mem_pestat] mean and std.dev: (139.67, 46.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (219.19, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.553 CPU sec, 5.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12267, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 99, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (140.52, 112.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (217.73, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.971 CPU sec, 4.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12077, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 104, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (121.82, 86.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 191, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (215.41, 99.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.908 CPU sec, 6.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 11993, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 135, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (163.83, 107.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (215.65, 99.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.244 CPU sec, 5.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11793, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 192, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (179.00, 86.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (219.16, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.270 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12497, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 150, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (167.12, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.68, 107.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.473 CPU sec, 5.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12449, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 137, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (162.05, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (225.10, 107.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.043 CPU sec, 5.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12522, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 99, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (138.00, 100.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (222.47, 105.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.841 CPU sec, 5.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12401, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 156, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (187.85, 149.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (222.79, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.919 CPU sec, 5.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12369, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 125, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (135.20, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (221.90, 106.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.857 CPU sec, 5.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12347, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 94, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (103.73, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (226.25, 108.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.173 CPU sec, 5.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12358, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 277, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (218.58, 113.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (227.53, 108.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.546 CPU sec, 4.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12317, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 126, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (132.22, 77.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.59, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.992 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12332, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 150, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (153.12, 108.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (222.97, 106.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.233 CPU sec, 4.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12346, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 180, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (189.69, 89.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.72, 108.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.133 CPU sec, 5.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12579, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 213, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (228.15, 179.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (223.56, 106.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.179 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12407, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 138, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (141.00, 84.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (221.30, 104.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.295 CPU sec, 5.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12260, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 169, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (147.53, 62.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (215.54, 101.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.389 CPU sec, 5.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11866, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 140, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (177.74, 117.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 190, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (213.94, 99.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.223 CPU sec, 5.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11710, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 98, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (129.75, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (216.15, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.114 CPU sec, 6.167 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11784, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 130, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (129.57, 45.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (222.97, 105.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.814 CPU sec, 5.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12534, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (136.70, 90.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (226.54, 109.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.465 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12323, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (136.87, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.43, 106.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.533 CPU sec, 5.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12391, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (131.77, 46.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (223.30, 106.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.457 CPU sec, 5.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12482, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 159, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (178.56, 114.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.53, 105.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.193 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12614, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 110, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (175.57, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (223.00, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.238 CPU sec, 4.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12280, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 136, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (159.44, 105.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (227.19, 109.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.874 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12353, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 107, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (105.20, 45.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (226.52, 110.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.857 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12385, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 133, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (122.09, 67.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (226.64, 108.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.035 CPU sec, 6.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12268, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 97, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (111.48, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.36, 107.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.846 CPU sec, 5.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12446, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 120, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (111.89, 44.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (226.74, 109.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.726 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12514, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 130, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (151.50, 124.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 199, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (225.27, 106.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.195 CPU sec, 4.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 12414, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 148, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (168.81, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (219.31, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.537 CPU sec, 6.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12190, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 158, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (154.00, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (217.19, 102.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.913 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12001, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 89, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (154.68, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (216.78, 101.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.516 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11840, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 168, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (148.00, 98.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (217.55, 100.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.698 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12049, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 182, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (181.18, 87.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (224.60, 108.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.711 CPU sec, 6.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12320, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 146, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (131.33, 64.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 198, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (225.81, 109.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.156 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12565, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (133.41, 64.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (224.28, 107.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.307 CPU sec, 5.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12555, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 171, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (183.04, 91.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (222.77, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.300 CPU sec, 4.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12480, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 142, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (154.62, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (222.23, 105.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.352 CPU sec, 5.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12562, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 105, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (148.77, 103.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (224.16, 107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.992 CPU sec, 7.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12452, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 158, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (160.00, 70.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (225.92, 110.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.729 CPU sec, 5.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12443, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (141.60, 65.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.82, 107.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.187 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12391, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (142.39, 104.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.16, 107.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.832 CPU sec, 5.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12447, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (126.55, 60.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (225.21, 107.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.135 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12495, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 120, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (144.29, 91.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 198, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.87, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.068 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12182, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (138.83, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (216.91, 101.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.885 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12375, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 122, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (162.80, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (215.44, 101.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.829 CPU sec, 4.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12200, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 139, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (140.68, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 189, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (212.17, 98.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.578 CPU sec, 5.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11747, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (174.40, 115.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 188, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (211.76, 96.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.182 CPU sec, 5.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11843, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 95, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (109.61, 71.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 189, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (213.34, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.016 CPU sec, 5.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12441, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 184, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (171.05, 91.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (221.26, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.175 CPU sec, 4.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12360, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 139, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (143.57, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (221.40, 105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.083 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12349, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 102, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (147.50, 98.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (222.50, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.036 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12376, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (115.24, 73.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (220.28, 105.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.331 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12379, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (143.33, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (219.49, 103.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.966 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12494, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (122.00, 68.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (223.98, 107.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.781 CPU sec, 5.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12281, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 152, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (152.58, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (223.84, 106.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.367 CPU sec, 5.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12265, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 115, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (161.44, 131.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (224.09, 106.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.688 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12384, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (114.75, 46.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (223.03, 107.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.433 CPU sec, 5.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12404, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 161, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (161.21, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (220.22, 105.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.884 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12573, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 179, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (154.88, 61.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 195, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (220.34, 103.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.008 CPU sec, 4.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12363, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (140.29, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (216.76, 101.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.083 CPU sec, 4.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12247, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 142, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (129.05, 63.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 190, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (215.98, 101.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.215 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11974, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 151, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (173.15, 90.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 188, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (211.50, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.785 CPU sec, 5.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 11625, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 188, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (210.03, 94.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 162.685 CPU sec, 6.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12112, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (134.12, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (219.72, 103.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.338 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12402, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (130.38, 95.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (223.53, 106.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.580 CPU sec, 5.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12391, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (108.00, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.45, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.997 CPU sec, 5.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12485, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (138.62, 84.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (222.91, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.867 CPU sec, 5.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12416, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (135.19, 56.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (220.94, 105.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.004 CPU sec, 6.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12492, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (188.44, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (221.56, 105.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.369 CPU sec, 5.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12341, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 127, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (156.06, 109.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 194, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (222.16, 106.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.677 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12492, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 167, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (164.18, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (223.09, 106.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.032 CPU sec, 5.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12505, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (123.56, 64.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (224.26, 107.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.957 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12463, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 183, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (177.33, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (223.39, 107.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.727 CPU sec, 5.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12505, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 158, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (174.94, 93.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 199, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (226.22, 109.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.503 CPU sec, 5.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12331, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (133.16, 57.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (217.94, 102.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.389 CPU sec, 6.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12423, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 132, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (162.62, 100.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (218.18, 102.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.762 CPU sec, 4.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12257, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 136, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (148.75, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (217.01, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.657 CPU sec, 4.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12081, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 138, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (142.69, 87.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 191, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (215.54, 99.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.271 CPU sec, 5.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11792, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 109, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (109.18, 51.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 190, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (215.77, 99.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.775 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12446, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 114, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (115.00, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (222.08, 104.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.182 CPU sec, 4.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12460, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 161, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (189.68, 119.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (223.77, 106.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.182 CPU sec, 5.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12333, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 112, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (131.50, 76.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (221.34, 105.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.920 CPU sec, 5.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12575, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 105, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 237) [M::mem_pestat] mean and std.dev: (108.50, 44.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (220.89, 104.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.092 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12520, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 147, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (164.73, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (220.78, 105.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.981 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12260, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 95, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (115.71, 70.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (222.53, 105.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.442 CPU sec, 5.936 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12402, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 147, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (157.35, 103.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (222.19, 105.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.347 CPU sec, 6.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12422, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 136, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (142.20, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.40, 107.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.795 CPU sec, 4.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12447, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 141, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (157.60, 89.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (223.32, 106.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.405 CPU sec, 6.396 real sec [M::process] read 32010 sequences (4833510 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12359, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 116, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (152.80, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 196, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (221.40, 104.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.078 CPU sec, 5.741 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 5991, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (224.14, 107.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 32010 reads in 59.335 CPU sec, 2.486 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21184\tSM:UG10S2P520\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21184_HLKNYBGXX_UG10S2P520_R1.fastq.gz 5347_7332_21184_HLKNYBGXX_UG10S2P520_R2.fastq.gz [main] Real time: 2072.279 sec; CPU: 53225.130 sec Finished alignement of UG10S2P520 at Wed Apr 3 19:40:56 2019 Sorting BAM file started at Wed Apr 3 19:40:56 2019 samtools sort -@ 30 -m 4G UG10S2P520_unsorted.bam -o UG10S2P520.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 19:40:56 2019 rm UG10S2P520_unsorted.bam rm 5347_7332_21184_HLKNYBGXX_UG10S2P520_R1.fastq.gz rm 5347_7332_21184_HLKNYBGXX_UG10S2P520_R2.fastq.gz samtools index UG10S2P520.bam Finished BAM file generation of the sample - UG10S2P520 Processing fastq files for the sample - UG10S2P535 Reading 5347_7332_21185_HLKNYBGXX_UG10S2P535_R1.fastq.gz 5347_7332_21185_HLKNYBGXX_UG10S2P535_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21185\tSM:UG10S2P535\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21185_HLKNYBGXX_UG10S2P535_R1.fastq.gz 5347_7332_21185_HLKNYBGXX_UG10S2P535_R2.fastq.gz | samtools view -Sb1 - > UG10S2P535_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13473, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 126, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (135.65, 79.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.69, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.393 CPU sec, 7.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13624, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 72, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (128.36, 101.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.56, 87.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.888 CPU sec, 5.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13623, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (127.32, 81.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.83, 88.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.492 CPU sec, 6.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13727, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 132, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (160.86, 115.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.34, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.068 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13757, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 139, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (169.54, 89.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.75, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.402 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13754, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 94, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (129.12, 84.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.67, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.662 CPU sec, 6.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13751, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (121.29, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.94, 87.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.655 CPU sec, 6.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13702, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 105, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (101.75, 38.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.90, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.550 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13584, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 96, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (106.32, 56.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.73, 89.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.784 CPU sec, 6.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13849, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 110, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (94.92, 46.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (195.59, 89.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.691 CPU sec, 6.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13771, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 153, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (171.88, 103.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.46, 89.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.465 CPU sec, 4.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13814, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 128, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (154.26, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.29, 87.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.147 CPU sec, 6.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13742, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 129, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (170.65, 133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.85, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.818 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13822, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 122, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (117.86, 52.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.26, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.643 CPU sec, 5.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13786, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 89, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (127.09, 90.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.97, 89.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.109 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13774, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 97, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (110.90, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.97, 89.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.649 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13804, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 129, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (140.33, 87.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.56, 87.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.694 CPU sec, 5.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13887, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 115, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (114.25, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.77, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.419 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13764, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 127, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (134.17, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.43, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.108 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13723, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 151, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (143.28, 85.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.98, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.720 CPU sec, 5.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13990, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 111, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (117.19, 56.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.99, 87.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.263 CPU sec, 5.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13693, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 135, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (132.38, 69.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.96, 86.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.438 CPU sec, 5.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13684, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 166, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (165.26, 82.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.81, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.295 CPU sec, 5.010 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13456, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 182, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (144.25, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.05, 88.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.311 CPU sec, 6.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13545, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 127, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (144.11, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.19, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.837 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13583, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 126, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (141.00, 67.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.74, 87.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.913 CPU sec, 6.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13686, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (109.32, 60.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.05, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.346 CPU sec, 5.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13795, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 129, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (150.33, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (194.89, 89.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.742 CPU sec, 5.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13556, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 83, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (75.05, 38.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.48, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.929 CPU sec, 5.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13591, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 145, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (153.40, 108.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.15, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.251 CPU sec, 5.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13600, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 94, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (111.59, 52.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.73, 88.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.236 CPU sec, 5.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13865, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (127.35, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (194.64, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.620 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13696, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 112, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (133.88, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.84, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.390 CPU sec, 4.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13643, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 115, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (125.16, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.72, 90.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.468 CPU sec, 6.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13638, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 111, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (106.44, 58.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.74, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.237 CPU sec, 4.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13854, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 132, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (118.11, 57.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.01, 86.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.196 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13752, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 116, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (121.95, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.42, 88.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.049 CPU sec, 5.173 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13906, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 113, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (121.05, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.78, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.627 CPU sec, 5.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13702, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (133.94, 85.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.11, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.257 CPU sec, 3.909 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13797, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 166, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (167.40, 86.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.96, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.968 CPU sec, 5.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13709, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (138.62, 81.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.19, 85.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.100 CPU sec, 4.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13801, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 147, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (159.76, 96.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.84, 85.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.841 CPU sec, 6.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13593, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (131.68, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.21, 84.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.153 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13674, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 135, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (141.62, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.28, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.145 CPU sec, 5.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13685, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 125, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (143.93, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.60, 85.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.873 CPU sec, 6.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13727, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (137.17, 74.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.66, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.498 CPU sec, 5.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13342, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (150.60, 96.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.95, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.554 CPU sec, 6.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13388, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 112, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (122.06, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.45, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.326 CPU sec, 7.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13507, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 119, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (134.53, 57.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.01, 89.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.076 CPU sec, 5.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13673, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (116.00, 60.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.37, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.838 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13635, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 105, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (131.67, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.43, 87.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.515 CPU sec, 4.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13705, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 158, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (158.35, 93.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.88, 87.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.399 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13580, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 134, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (133.50, 74.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.43, 90.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.840 CPU sec, 4.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13716, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (145.08, 104.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.40, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.994 CPU sec, 4.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13888, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 127, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (147.76, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.98, 88.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.665 CPU sec, 4.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13798, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 129, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (154.09, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.15, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.266 CPU sec, 5.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13836, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 151, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (132.65, 63.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.84, 89.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.011 CPU sec, 4.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13757, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (129.26, 67.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.52, 89.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.098 CPU sec, 4.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13812, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 112, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (135.12, 76.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.69, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.661 CPU sec, 4.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13791, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 91, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (86.86, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.47, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.732 CPU sec, 4.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13650, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 93, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (99.85, 55.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.29, 87.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.424 CPU sec, 6.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13684, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (156.29, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.61, 87.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.355 CPU sec, 5.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13556, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (128.14, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.67, 86.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.867 CPU sec, 4.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13684, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (153.03, 82.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (190.23, 84.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.600 CPU sec, 4.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13644, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 115, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (150.93, 104.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.45, 87.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.130 CPU sec, 5.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13674, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 136, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (150.44, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (191.94, 85.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.634 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13744, 10, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 108, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (142.00, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.54, 84.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 2713, 6799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19261) [M::mem_pestat] mean and std.dev: (3491.60, 3771.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25492) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 122.836 CPU sec, 6.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13643, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 157, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (132.64, 62.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.62, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.367 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13362, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 113, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (149.05, 91.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.06, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.372 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13458, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 151, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (161.68, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.94, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.425 CPU sec, 4.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13835, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 111, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (113.71, 59.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.79, 85.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.043 CPU sec, 5.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13685, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 123, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (134.62, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.07, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.355 CPU sec, 4.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13661, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 164, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (155.19, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.10, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.273 CPU sec, 4.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13633, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (162.15, 95.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.69, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.597 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13788, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 110, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (121.14, 48.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.34, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.724 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13600, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (112.11, 56.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.29, 87.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.913 CPU sec, 5.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13641, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 109, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (126.16, 73.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.47, 87.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.087 CPU sec, 5.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13782, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 117, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (112.39, 46.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.17, 87.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.614 CPU sec, 5.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13895, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (122.00, 61.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.23, 88.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.353 CPU sec, 4.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13632, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.95, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.829 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13861, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 143, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (154.70, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.55, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.160 CPU sec, 5.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13638, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (136.45, 83.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.90, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.922 CPU sec, 4.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13663, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 161, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (152.80, 72.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.73, 87.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.075 CPU sec, 4.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13558, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (130.65, 38.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.82, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.893 CPU sec, 5.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13705, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 91, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (123.17, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.49, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.499 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13578, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 102, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (143.48, 83.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (189.68, 85.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.734 CPU sec, 4.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13690, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 184, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (187.42, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.47, 85.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.777 CPU sec, 5.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13773, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (148.59, 85.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.27, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.230 CPU sec, 4.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13644, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 90, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (103.50, 57.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (190.29, 84.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.473 CPU sec, 5.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13707, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 127, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (136.48, 70.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.16, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.331 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13641, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (154.38, 62.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.63, 86.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.100 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13447, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 122, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (131.28, 56.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.25, 83.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.850 CPU sec, 6.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13452, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 151, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (173.75, 82.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.29, 86.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.762 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13457, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (109.65, 48.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.58, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.480 CPU sec, 5.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13611, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 161, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (154.29, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.42, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.796 CPU sec, 6.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13667, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 154, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (140.62, 70.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.25, 85.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.561 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13734, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (152.56, 89.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.55, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.714 CPU sec, 6.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13631, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 177, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (176.30, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.42, 87.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.483 CPU sec, 5.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13717, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 173, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (182.00, 113.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.47, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.625 CPU sec, 6.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13729, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 137, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (137.00, 61.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.88, 87.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.156 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13687, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (149.50, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.99, 85.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.344 CPU sec, 6.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13762, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 164, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (164.12, 76.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.91, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.473 CPU sec, 5.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13646, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (151.78, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.79, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.462 CPU sec, 6.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 13654, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (132.49, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.62, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.958 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13771, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 174, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (163.05, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.59, 85.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.829 CPU sec, 6.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13866, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 135, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (148.59, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.27, 86.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.136 CPU sec, 5.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13475, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 113, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (142.30, 101.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.92, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.748 CPU sec, 7.138 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13704, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 98, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (109.25, 60.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.45, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.333 CPU sec, 5.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13722, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 118, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (120.75, 43.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.48, 87.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.704 CPU sec, 6.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13522, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 140, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (140.22, 73.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.37, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.467 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13810, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 119, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (118.25, 47.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.85, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.929 CPU sec, 6.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13661, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 126, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (149.23, 98.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (189.99, 84.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.632 CPU sec, 5.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13540, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (148.23, 81.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.89, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.419 CPU sec, 5.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13689, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 160, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (155.96, 81.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (190.74, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.639 CPU sec, 6.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13374, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 102, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (126.08, 69.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.05, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.672 CPU sec, 5.928 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13407, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 111, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (142.83, 87.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.55, 83.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.217 CPU sec, 6.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13407, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 137, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (122.31, 63.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.66, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.924 CPU sec, 5.832 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13583, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 84, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (84.17, 23.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.21, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.515 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13621, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (149.34, 86.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.37, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.446 CPU sec, 7.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13571, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 91, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (109.13, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.18, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.033 CPU sec, 6.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13642, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (98.53, 67.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.63, 85.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.218 CPU sec, 7.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13646, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 176, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (170.47, 61.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.60, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.510 CPU sec, 6.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13626, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 139, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (149.07, 104.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (192.59, 88.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.297 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13736, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 96, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (88.11, 47.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.36, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.909 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13674, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 110, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (151.96, 112.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.36, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.192 CPU sec, 5.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13528, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 136, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (129.00, 68.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.57, 86.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.562 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13771, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 107, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (120.19, 63.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (192.07, 84.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.394 CPU sec, 6.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13779, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (117.19, 59.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.83, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.028 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13647, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 141, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (150.56, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.40, 84.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.317 CPU sec, 6.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13827, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 122, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (130.04, 77.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.72, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.786 CPU sec, 7.148 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13540, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 144, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (153.79, 109.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (191.11, 87.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.670 CPU sec, 5.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13650, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 141, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (148.32, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.12, 84.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.449 CPU sec, 6.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13806, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (155.11, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.48, 85.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.326 CPU sec, 5.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13658, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 154, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (147.36, 81.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.70, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.988 CPU sec, 4.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13691, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 135, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (137.86, 58.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.46, 84.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.318 CPU sec, 6.043 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13573, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 112, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (120.41, 64.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.20, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.327 CPU sec, 6.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13631, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (149.00, 92.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.91, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.887 CPU sec, 6.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13810, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (172.94, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (195.02, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.372 CPU sec, 5.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13741, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 103, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (130.60, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.76, 90.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.485 CPU sec, 5.171 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13615, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 88, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (111.90, 51.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.86, 89.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.277 CPU sec, 5.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13869, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 133, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (167.87, 114.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.46, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.962 CPU sec, 5.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13785, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (129.55, 90.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (192.51, 85.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.380 CPU sec, 6.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13676, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 145, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (143.00, 68.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.56, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.194 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13665, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (120.57, 63.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.45, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.333 CPU sec, 7.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13609, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 98, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (87.44, 49.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.36, 91.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.850 CPU sec, 6.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13665, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 105, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (131.62, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.15, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.237 CPU sec, 6.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13668, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 133, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (132.33, 51.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.22, 88.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.908 CPU sec, 6.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13788, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 128, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (142.04, 59.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (193.49, 88.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.722 CPU sec, 6.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13778, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 91, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (121.30, 83.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (192.55, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.158 CPU sec, 6.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13630, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (145.50, 83.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.93, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.413 CPU sec, 5.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13592, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 131, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (153.12, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.43, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.748 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13757, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 109, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (128.78, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.57, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.876 CPU sec, 6.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13702, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 116, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (157.81, 109.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.51, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.330 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13600, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 148, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (139.10, 49.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.27, 83.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.934 CPU sec, 5.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13567, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 146, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (177.29, 131.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.54, 85.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.438 CPU sec, 5.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13729, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (123.76, 74.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.70, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.676 CPU sec, 7.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13503, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 126, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (113.69, 51.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (189.80, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.117 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13464, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 129, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (143.11, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.43, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.511 CPU sec, 5.631 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13341, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (118.22, 53.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.08, 83.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.182 CPU sec, 6.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13000, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (157.06, 60.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (187.55, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.701 CPU sec, 5.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13735, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 105, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (124.94, 53.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.62, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.226 CPU sec, 4.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13731, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 107, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (147.52, 83.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.53, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.512 CPU sec, 5.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13615, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 122, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (129.96, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (195.88, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.273 CPU sec, 7.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13686, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 167, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (166.58, 70.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.85, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.251 CPU sec, 5.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13709, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 102, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (129.12, 85.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (197.05, 89.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.904 CPU sec, 7.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13723, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 136, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (164.88, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.46, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.137 CPU sec, 4.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13706, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 156, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (159.41, 84.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.48, 89.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.792 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13660, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 133, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (131.23, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.79, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.290 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13787, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 136, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (165.50, 100.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (195.72, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.212 CPU sec, 5.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13686, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (151.83, 90.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (195.41, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.510 CPU sec, 4.799 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13783, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 131, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (154.54, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.39, 90.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.998 CPU sec, 4.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13868, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (121.63, 52.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.33, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.417 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13762, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 133, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (145.56, 96.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.22, 89.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.112 CPU sec, 5.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13829, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 80, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (87.29, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.83, 87.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.409 CPU sec, 5.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13654, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 109, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (105.77, 64.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.19, 87.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.341 CPU sec, 6.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13637, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 93, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (113.65, 54.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (194.05, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.774 CPU sec, 6.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13522, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 143, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (123.15, 47.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.72, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.428 CPU sec, 5.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13535, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 177, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (175.44, 100.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.78, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.432 CPU sec, 6.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13407, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 139, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (136.95, 54.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.21, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.619 CPU sec, 6.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13458, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 127, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (126.08, 62.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.25, 84.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.242 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13406, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 90, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (115.58, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (187.97, 82.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.664 CPU sec, 6.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13095, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 121, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (146.00, 53.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.04, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.860 CPU sec, 5.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13099, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 108, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (107.89, 52.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.97, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.362 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13528, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 182, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (174.91, 91.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (197.49, 91.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.663 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13603, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (194.00, 154.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.12, 89.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.028 CPU sec, 5.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13440, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 135, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (154.48, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 174, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (196.82, 89.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.674 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13752, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 149, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (139.88, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (196.94, 90.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.329 CPU sec, 6.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13659, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 98, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (143.12, 101.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.85, 90.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.657 CPU sec, 6.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13684, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 113, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (137.63, 78.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.74, 89.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.303 CPU sec, 6.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13790, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (157.50, 112.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.33, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.720 CPU sec, 6.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13610, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (161.00, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (196.62, 89.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.330 CPU sec, 7.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13718, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (145.35, 107.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (195.32, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.877 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13548, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (120.05, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (197.38, 90.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.154 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13633, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 92, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (106.43, 58.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.38, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.025 CPU sec, 6.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13636, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 142, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (139.57, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.08, 88.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.177 CPU sec, 7.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13624, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 126, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (116.29, 57.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.51, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.213 CPU sec, 6.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13883, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 140, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (150.55, 75.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.25, 88.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.399 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13876, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 143, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (147.11, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.47, 86.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.222 CPU sec, 5.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13708, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 100, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (140.36, 94.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.74, 87.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.735 CPU sec, 6.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13577, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (149.76, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.06, 86.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.586 CPU sec, 5.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13783, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 133, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (118.84, 68.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.75, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.143 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13662, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (135.77, 109.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.31, 83.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.163 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13412, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 154, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (137.87, 70.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.06, 83.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.968 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13418, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (134.83, 102.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.73, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.976 CPU sec, 5.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13221, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (150.94, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.14, 83.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.094 CPU sec, 6.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13303, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 179, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (163.92, 73.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.71, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.277 CPU sec, 6.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13641, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 101, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (98.08, 41.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.39, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.238 CPU sec, 5.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13619, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 153, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (159.27, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (192.39, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.152 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13773, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 155, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (143.40, 60.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.55, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.754 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13639, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 139, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (162.39, 96.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (192.75, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.518 CPU sec, 5.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13592, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (127.04, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (194.46, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.373 CPU sec, 5.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13577, 0, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 162, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (143.00, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.96, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.331 CPU sec, 5.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13613, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 92, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (115.14, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.75, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.652 CPU sec, 5.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13577, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 152, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (147.67, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.34, 87.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.764 CPU sec, 5.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13687, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.15, 85.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.23, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.309 CPU sec, 6.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13697, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 105, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (125.42, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.29, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.754 CPU sec, 6.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13722, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (128.70, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.94, 87.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.271 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13637, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 120, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (133.43, 81.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.82, 88.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.901 CPU sec, 8.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13819, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (164.80, 133.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.85, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.465 CPU sec, 5.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13606, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 149, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (158.72, 76.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.05, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.694 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13728, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.22, 85.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 149.127 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13719, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 77, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (85.90, 44.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.29, 86.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.466 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13735, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (138.83, 86.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.63, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.230 CPU sec, 5.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13757, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (146.50, 71.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.31, 85.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.685 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13520, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 133, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (144.10, 85.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.78, 83.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.534 CPU sec, 5.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13570, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 142, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (129.64, 58.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.46, 84.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.860 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13353, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 122, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (138.73, 74.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.90, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.877 CPU sec, 6.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13213, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 128, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (127.47, 55.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.09, 82.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.225 CPU sec, 6.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13506, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 152, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (158.38, 65.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (191.56, 84.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.124 CPU sec, 6.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13543, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (123.33, 75.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.75, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.958 CPU sec, 6.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13610, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (125.95, 70.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (195.03, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.696 CPU sec, 5.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13735, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 167, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (179.38, 137.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.17, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.814 CPU sec, 5.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13722, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (124.09, 66.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.04, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.751 CPU sec, 5.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13581, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 143, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (114.58, 83.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.56, 88.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.088 CPU sec, 5.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13794, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (122.90, 59.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.73, 88.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.268 CPU sec, 5.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13561, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 133, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (147.00, 83.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.06, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.682 CPU sec, 6.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13591, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 159, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (158.35, 102.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.45, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.589 CPU sec, 5.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13765, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (121.88, 60.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (195.16, 89.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.332 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13642, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 95, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (105.95, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.52, 88.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.601 CPU sec, 5.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13641, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 138, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (164.44, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.35, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.232 CPU sec, 5.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13817, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (109.85, 60.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.23, 88.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.279 CPU sec, 7.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13788, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 95, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (141.50, 106.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.40, 86.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.462 CPU sec, 5.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13676, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 192, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (193.47, 130.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.36, 87.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.434 CPU sec, 6.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13626, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 115, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (122.00, 58.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.50, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.831 CPU sec, 6.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13726, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (151.48, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.24, 85.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.163 CPU sec, 5.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13625, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 98, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (105.50, 59.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.51, 83.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.605 CPU sec, 5.089 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13654, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 138, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (142.94, 58.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (189.56, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.111 CPU sec, 5.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13735, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 128, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (134.08, 70.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (188.32, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.331 CPU sec, 5.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13521, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (128.56, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (186.56, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.456 CPU sec, 5.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13046, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (147.17, 86.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (186.03, 80.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.971 CPU sec, 6.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13090, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 124, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (147.69, 89.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (186.61, 81.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.909 CPU sec, 7.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13720, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 148, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (163.41, 72.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (194.86, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.150 CPU sec, 6.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13561, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (142.60, 60.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.75, 89.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.288 CPU sec, 6.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13527, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 146, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (117.17, 61.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (195.67, 89.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.301 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13650, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 131, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (155.68, 69.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.73, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.795 CPU sec, 6.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13589, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 124, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (122.82, 66.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.94, 89.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.215 CPU sec, 6.131 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13512, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 119, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (130.61, 67.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.72, 88.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.344 CPU sec, 6.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13446, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (142.00, 76.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.60, 88.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.760 CPU sec, 5.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13811, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 159, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (156.52, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (193.64, 89.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.847 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13699, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (91.20, 30.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.60, 89.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.558 CPU sec, 6.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13708, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 102, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (114.77, 55.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (194.95, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.585 CPU sec, 6.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13673, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 127, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (130.50, 57.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.63, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.062 CPU sec, 6.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13681, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (120.61, 47.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.43, 88.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.501 CPU sec, 6.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13596, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (148.29, 86.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.88, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.936 CPU sec, 6.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13626, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 108, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (142.76, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.02, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.938 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13680, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 108, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (123.09, 66.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.55, 84.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.577 CPU sec, 5.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13591, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 137, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (144.97, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.76, 86.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.346 CPU sec, 5.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13706, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (159.81, 96.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.40, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.447 CPU sec, 5.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13734, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 121, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (123.45, 74.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.55, 83.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.715 CPU sec, 6.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13561, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (132.85, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.45, 85.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.850 CPU sec, 6.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13546, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (174.95, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.37, 85.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.387 CPU sec, 6.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13420, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 133, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (146.74, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (187.18, 81.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.248 CPU sec, 5.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13214, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 120, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (107.44, 47.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 167, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (186.22, 81.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.413 CPU sec, 6.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13413, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 134, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (148.74, 104.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.46, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.916 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13722, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 125, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (141.71, 67.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.01, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.881 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13592, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 101, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (103.95, 63.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.65, 85.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.899 CPU sec, 7.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13783, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 176, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (164.91, 105.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (189.93, 85.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.354 CPU sec, 6.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13778, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 160, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (170.74, 72.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.63, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.740 CPU sec, 6.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13761, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 145, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (161.75, 100.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.28, 84.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.903 CPU sec, 5.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13679, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 129, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (138.30, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.30, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.477 CPU sec, 5.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13745, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 146, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (150.95, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (194.65, 87.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.214 CPU sec, 7.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13923, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 115, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (114.89, 47.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (196.00, 90.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.362 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13789, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 138, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (155.65, 100.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.50, 88.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.124 CPU sec, 6.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13566, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 175, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (179.88, 83.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.26, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.341 CPU sec, 4.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13773, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (113.36, 43.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.12, 87.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.749 CPU sec, 5.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13695, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (120.00, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.82, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.106 CPU sec, 5.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13755, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 132, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (133.93, 70.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.67, 89.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.187 CPU sec, 5.515 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13858, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 84, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (90.50, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.20, 86.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.366 CPU sec, 5.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13301, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (126.30, 77.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.83, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.050 CPU sec, 5.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13374, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (178.61, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.71, 87.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.158 CPU sec, 5.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13721, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 89, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (104.86, 62.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.47, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.690 CPU sec, 6.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13602, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 142, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (134.35, 64.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.77, 86.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.474 CPU sec, 6.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 13539, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 112, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (162.40, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.67, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.081 CPU sec, 5.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13625, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 114, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (115.43, 78.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (195.34, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.777 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13639, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 177, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (153.47, 86.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.82, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.751 CPU sec, 5.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13712, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 100, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (122.42, 90.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.65, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.132 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13638, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 151, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (141.21, 69.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.37, 87.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.142 CPU sec, 6.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13854, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (142.97, 78.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (194.10, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.088 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13609, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 117, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (133.04, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.75, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.808 CPU sec, 5.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13660, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 111, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (123.31, 55.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.78, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.115 CPU sec, 6.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13672, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 135, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (142.74, 89.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.02, 85.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.632 CPU sec, 6.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13679, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 135, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (149.00, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.10, 86.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.894 CPU sec, 4.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13730, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 105, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (144.13, 84.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.02, 86.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.911 CPU sec, 5.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13823, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 111, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (139.96, 81.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (194.42, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.094 CPU sec, 6.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13724, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 108, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (123.22, 65.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.32, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.790 CPU sec, 6.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13650, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (121.67, 53.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (195.62, 89.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.617 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13872, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 98, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (119.22, 72.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.33, 89.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.979 CPU sec, 6.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13782, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (148.33, 59.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.70, 87.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.492 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13553, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (183.12, 106.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.37, 89.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.814 CPU sec, 6.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13801, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 137, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (138.43, 55.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.88, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.569 CPU sec, 6.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13611, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 128, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (155.68, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.66, 87.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.394 CPU sec, 5.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13477, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (124.00, 53.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.50, 86.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.085 CPU sec, 5.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13655, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 137, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (170.79, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (192.39, 86.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.591 CPU sec, 5.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13536, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 190, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (175.59, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.39, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.055 CPU sec, 5.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13554, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 113, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (136.88, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.33, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.710 CPU sec, 6.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13765, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (175.70, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.87, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.534 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13564, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 135, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (126.82, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.74, 88.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.519 CPU sec, 7.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13738, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (141.93, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.53, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.918 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13855, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 164, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (163.79, 73.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.79, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.974 CPU sec, 6.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13651, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 164, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (180.42, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.35, 84.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.288 CPU sec, 6.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13596, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (132.10, 88.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.30, 84.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.037 CPU sec, 6.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13843, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (127.68, 58.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.17, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.978 CPU sec, 6.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13618, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 109, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (109.22, 52.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (188.88, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.625 CPU sec, 5.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13578, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 99, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (118.54, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.22, 83.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.984 CPU sec, 7.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13798, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 104, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (100.62, 49.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.38, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.246 CPU sec, 7.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13807, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 139, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (196.42, 143.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.55, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.907 CPU sec, 6.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13713, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 122, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (146.12, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.11, 88.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.140 CPU sec, 5.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13686, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 123, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (137.68, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.88, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.217 CPU sec, 6.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13761, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (180.38, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.92, 87.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.479 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13686, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (133.23, 85.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (189.98, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.390 CPU sec, 6.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13875, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 132, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (140.57, 53.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (192.60, 86.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.586 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13835, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 84, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (87.11, 33.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.47, 87.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.385 CPU sec, 5.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13484, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 125, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (144.09, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (191.47, 85.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.238 CPU sec, 6.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13470, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (120.18, 60.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.13, 85.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.399 CPU sec, 6.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13596, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (167.05, 109.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (192.88, 88.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.516 CPU sec, 6.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13750, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 131, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (133.90, 81.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.34, 88.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.214 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13690, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 132, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (150.89, 84.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.12, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.710 CPU sec, 7.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13792, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 127, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (142.74, 59.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (195.37, 89.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.493 CPU sec, 7.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13777, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 143, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (152.39, 60.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.44, 88.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.738 CPU sec, 5.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13795, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 113, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (122.23, 67.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.92, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.367 CPU sec, 7.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13560, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 128, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (141.00, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.05, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.361 CPU sec, 7.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13868, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 161, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (157.39, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.59, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.187 CPU sec, 6.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13720, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 135, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (194.69, 127.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.26, 84.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.486 CPU sec, 5.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13751, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 111, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (105.83, 35.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.02, 85.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.761 CPU sec, 6.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13738, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 136, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (142.56, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.30, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.494 CPU sec, 5.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13617, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (164.04, 113.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (190.44, 84.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.042 CPU sec, 5.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13763, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (130.07, 55.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (192.07, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.427 CPU sec, 6.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13892, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 137, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (128.25, 70.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.63, 86.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.598 CPU sec, 6.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13714, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 126, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (121.13, 48.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.75, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.839 CPU sec, 5.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13855, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 87, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (86.73, 49.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.71, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.812 CPU sec, 4.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13707, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 145, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (159.10, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.27, 87.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.916 CPU sec, 5.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13792, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 106, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (118.67, 54.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.31, 85.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.327 CPU sec, 7.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13716, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 139, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (153.24, 100.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.67, 87.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.190 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13659, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 105, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (123.33, 80.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.59, 85.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.236 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13495, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 140, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (148.94, 73.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.39, 82.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.547 CPU sec, 4.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13514, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (131.57, 44.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (186.51, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.412 CPU sec, 5.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13397, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (122.94, 71.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.99, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.612 CPU sec, 5.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13634, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (120.23, 57.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.68, 85.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.067 CPU sec, 5.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13612, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 106, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (151.91, 86.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.48, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.815 CPU sec, 6.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13694, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 96, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (104.50, 58.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.07, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.237 CPU sec, 5.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13766, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 161, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (147.95, 66.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (192.08, 87.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.695 CPU sec, 5.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13661, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 100, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (118.39, 81.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.73, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.270 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13865, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 158, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (146.52, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.83, 85.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.641 CPU sec, 5.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13809, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (138.50, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.79, 84.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.678 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13812, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 111, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (146.73, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.91, 87.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.872 CPU sec, 6.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13781, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 93, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (118.47, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.10, 85.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.821 CPU sec, 5.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13589, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 100, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (125.60, 83.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.54, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.085 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13765, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 111, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (132.09, 79.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.82, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.729 CPU sec, 6.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13818, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 110, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (120.58, 53.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.51, 85.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.734 CPU sec, 5.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13563, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (135.17, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.82, 87.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.277 CPU sec, 5.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13728, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 155, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (170.72, 95.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.40, 86.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.768 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13630, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (164.39, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.59, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.246 CPU sec, 5.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13696, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 109, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (153.74, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.75, 84.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.637 CPU sec, 6.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13832, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 173, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (164.18, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.02, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.409 CPU sec, 6.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13727, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (143.05, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.24, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.092 CPU sec, 5.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13760, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 100, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (99.64, 30.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.08, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.693 CPU sec, 5.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13661, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (122.94, 63.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (189.52, 83.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.382 CPU sec, 6.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13478, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (122.74, 57.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.51, 83.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.611 CPU sec, 6.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13491, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 97, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (94.50, 29.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.99, 83.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.141 CPU sec, 6.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13677, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 126, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (148.63, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (190.13, 84.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.971 CPU sec, 6.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13578, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 97, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (112.96, 66.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (188.86, 84.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.425 CPU sec, 5.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13742, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (147.10, 68.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (188.51, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.498 CPU sec, 6.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13733, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 108, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (149.71, 100.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.33, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.650 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13849, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 130, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (139.54, 71.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (192.15, 86.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.938 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13618, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (128.38, 71.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.80, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.770 CPU sec, 7.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13785, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 121, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (132.92, 61.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.04, 85.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.607 CPU sec, 6.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13685, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 112, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (107.86, 60.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.53, 84.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.862 CPU sec, 6.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13723, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 132, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (155.50, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (187.29, 81.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.854 CPU sec, 7.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13671, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (110.45, 45.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.89, 83.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.486 CPU sec, 5.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13529, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 94, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (125.11, 65.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (187.90, 83.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.762 CPU sec, 5.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13744, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 103, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (122.04, 62.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (188.36, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.237 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13771, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (145.63, 99.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.28, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.601 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13729, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 140, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (139.33, 48.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.13, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.598 CPU sec, 5.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13599, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 95, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (137.13, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.50, 86.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.077 CPU sec, 5.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13609, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (118.85, 85.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.98, 86.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.913 CPU sec, 6.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13667, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 152, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (157.20, 99.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.52, 86.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.562 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13690, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (146.62, 110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.73, 86.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.202 CPU sec, 5.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13656, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 136, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (125.64, 52.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (190.63, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.421 CPU sec, 5.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13528, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (151.94, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (189.17, 83.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.962 CPU sec, 7.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13405, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 171, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (148.58, 59.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (190.41, 84.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.167 CPU sec, 6.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13555, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 99, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (106.53, 71.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.39, 83.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.796 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13669, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 144, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (133.05, 61.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.05, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.859 CPU sec, 5.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13731, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 108, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (115.81, 63.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.38, 84.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.548 CPU sec, 6.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13626, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 105, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (117.83, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.25, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.069 CPU sec, 5.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13567, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (152.09, 113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (192.10, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.959 CPU sec, 6.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13729, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 116, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (134.44, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.59, 84.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.060 CPU sec, 5.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13685, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 121, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (135.92, 69.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (191.33, 85.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.034 CPU sec, 6.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13673, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 119, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (145.23, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.59, 84.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.096 CPU sec, 5.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13726, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 123, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (121.29, 61.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (189.47, 84.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.797 CPU sec, 7.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13516, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 116, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (124.57, 63.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.51, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.060 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13553, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (108.57, 60.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (186.46, 80.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.697 CPU sec, 5.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13488, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 121, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (156.21, 108.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (187.28, 81.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.462 CPU sec, 7.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13480, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 114, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (107.14, 45.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (188.16, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.456 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13241, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 128, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (138.81, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.53, 85.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.957 CPU sec, 7.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13813, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 95, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (108.79, 67.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.18, 88.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.544 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13747, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 97, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (117.95, 70.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.29, 86.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.459 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13818, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 143, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (156.24, 62.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.52, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.343 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13446, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 111, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (118.92, 45.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.64, 88.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.728 CPU sec, 6.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13765, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 104, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (134.00, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.24, 86.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.847 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13680, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 171, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (151.14, 53.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.10, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.321 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13658, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 174, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (171.50, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.90, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.128 CPU sec, 6.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13677, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 132, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (129.06, 52.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.95, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.685 CPU sec, 6.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13527, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 124, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (125.56, 58.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (197.03, 90.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.815 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13591, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 145, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (168.63, 83.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.46, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.906 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13689, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 128, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (163.52, 100.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (196.31, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.852 CPU sec, 6.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13747, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 111, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (133.12, 77.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.12, 87.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.645 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13796, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 83, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (95.00, 41.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (194.67, 89.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.762 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13778, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 84, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (93.85, 53.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (193.39, 87.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.177 CPU sec, 7.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13686, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 102, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (118.70, 63.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.35, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.909 CPU sec, 5.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13729, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 131, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (122.21, 63.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.09, 86.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.375 CPU sec, 6.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13724, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 86, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (91.77, 55.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.08, 84.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.939 CPU sec, 5.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13681, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 117, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (143.06, 66.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (190.67, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.394 CPU sec, 6.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13621, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 157, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 283) [M::mem_pestat] mean and std.dev: (141.80, 29.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.60, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.896 CPU sec, 6.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13213, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 96, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (110.05, 55.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.93, 83.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.933 CPU sec, 7.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13297, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (130.60, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (189.69, 83.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.293 CPU sec, 7.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13360, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 109, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (145.68, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.79, 85.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.299 CPU sec, 6.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13809, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 186, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (209.53, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.55, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.917 CPU sec, 6.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13631, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 193, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (204.32, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (193.89, 87.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.001 CPU sec, 7.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13643, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 92, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (123.00, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.33, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.177 CPU sec, 5.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13709, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 155, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (174.04, 112.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.36, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.640 CPU sec, 6.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13667, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 108, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (140.53, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.31, 84.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.965 CPU sec, 5.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13677, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 94, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (121.93, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (194.32, 87.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.520 CPU sec, 6.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13698, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (155.76, 72.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (192.83, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.595 CPU sec, 5.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13584, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 159, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (149.80, 66.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.41, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.798 CPU sec, 5.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13620, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 113, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (118.33, 56.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (192.73, 88.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.351 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13721, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 142, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (181.06, 117.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (196.14, 90.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.907 CPU sec, 6.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13710, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 196, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (184.33, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.82, 88.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.366 CPU sec, 5.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13782, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 72, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (107.87, 83.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (194.55, 88.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.614 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13752, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (131.65, 63.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 174, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.81, 89.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.601 CPU sec, 6.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13625, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (144.00, 113.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (191.90, 86.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.671 CPU sec, 7.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13815, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 119, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (138.55, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.31, 87.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.051 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13693, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 155, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (143.33, 69.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (191.36, 85.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.589 CPU sec, 6.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13736, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 102, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (124.90, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.14, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.716 CPU sec, 5.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13718, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 108, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (143.40, 75.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.76, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.774 CPU sec, 6.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13541, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 138, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (133.06, 63.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.37, 83.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.034 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13422, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 137, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (138.05, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.92, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.968 CPU sec, 6.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13338, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (139.32, 84.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (188.76, 83.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.728 CPU sec, 6.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13264, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (122.67, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (191.05, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.036 CPU sec, 5.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13729, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (125.68, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.48, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.858 CPU sec, 7.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13914, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 88, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (102.94, 60.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.78, 88.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.603 CPU sec, 5.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13700, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 171, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (167.57, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (195.20, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.281 CPU sec, 5.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13587, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (149.38, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.49, 87.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.767 CPU sec, 5.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13752, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (142.67, 105.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.15, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.405 CPU sec, 6.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13846, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 101, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (99.86, 31.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (192.17, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.529 CPU sec, 6.304 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13633, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (139.96, 93.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.33, 88.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.976 CPU sec, 5.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13710, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 110, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (135.83, 73.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.44, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.339 CPU sec, 5.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13771, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (133.76, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (193.83, 87.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.866 CPU sec, 7.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13665, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 134, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (163.46, 106.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.17, 87.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.745 CPU sec, 5.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13847, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (151.08, 103.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.50, 86.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.925 CPU sec, 7.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13828, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 110, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (126.40, 65.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.47, 87.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.879 CPU sec, 5.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13654, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.77, 88.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.434 CPU sec, 6.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13833, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 150, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (166.63, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.76, 88.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.031 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13636, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 139, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (135.42, 74.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.16, 87.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.181 CPU sec, 7.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13738, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 112, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (157.95, 116.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (187.03, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.831 CPU sec, 7.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13579, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 140, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (143.18, 58.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.03, 83.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.069 CPU sec, 6.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13731, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 129, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (152.52, 69.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (187.78, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.725 CPU sec, 7.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13491, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 107, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (104.24, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (185.95, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.728 CPU sec, 6.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13436, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 107, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (123.95, 78.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.86, 82.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.213 CPU sec, 6.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13336, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 158, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (168.40, 78.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 165, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (182.31, 78.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.186 CPU sec, 7.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13093, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 142, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (142.29, 62.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (185.37, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.515 CPU sec, 5.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13741, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 143, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (139.59, 67.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (192.93, 87.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.749 CPU sec, 7.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13708, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 120, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (155.76, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.27, 88.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.470 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13684, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (125.82, 74.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.90, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.271 CPU sec, 6.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13721, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 153, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (163.35, 62.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.79, 87.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.614 CPU sec, 5.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13666, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (146.04, 79.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.76, 84.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.956 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13704, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 135, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (154.39, 101.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.37, 86.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.823 CPU sec, 5.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13820, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (138.00, 88.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.78, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.005 CPU sec, 5.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13818, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (131.94, 79.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.72, 86.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.621 CPU sec, 6.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13592, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (140.58, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (194.12, 87.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.475 CPU sec, 6.085 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13854, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 162, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (157.00, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (192.32, 87.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.790 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13556, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 99, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (119.81, 72.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.71, 87.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.978 CPU sec, 6.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13767, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 93, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (116.56, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (192.61, 86.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.358 CPU sec, 5.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13668, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 111, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (114.76, 77.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (194.90, 88.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.273 CPU sec, 6.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13749, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 115, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (142.75, 87.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.07, 86.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.559 CPU sec, 6.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13816, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (148.32, 65.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (189.75, 84.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.718 CPU sec, 6.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13814, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 109, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (116.84, 69.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 170, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.58, 85.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.589 CPU sec, 5.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13672, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 130, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (125.59, 62.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (187.93, 82.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.784 CPU sec, 6.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13672, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 109, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (128.37, 95.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (188.18, 83.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.439 CPU sec, 6.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13706, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 127, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (152.96, 80.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 167, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (186.01, 81.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.187 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13532, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 114, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (132.13, 60.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (183.18, 78.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.121 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13216, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 138, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (159.09, 92.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 165, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (182.42, 77.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.226 CPU sec, 6.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12914, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 142, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (164.75, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (184.27, 79.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.586 CPU sec, 7.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13697, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 137, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (148.36, 90.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.68, 86.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.477 CPU sec, 7.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13617, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 97, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (101.28, 52.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 173, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (193.07, 85.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.400 CPU sec, 6.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13814, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (124.50, 93.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (193.47, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.693 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13690, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 106, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (149.95, 107.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.03, 86.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.792 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13733, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 123, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (143.26, 67.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.11, 86.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.501 CPU sec, 6.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13796, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (155.50, 114.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (189.67, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.732 CPU sec, 11.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13822, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (123.72, 66.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.29, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.012 CPU sec, 5.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13811, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 111, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (143.88, 102.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.47, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.273 CPU sec, 6.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13729, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (119.15, 69.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (193.85, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.846 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13710, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 105, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (108.93, 45.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.11, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.690 CPU sec, 5.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13769, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 131, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (154.88, 102.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 172, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (194.09, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.124 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13735, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 103, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (99.50, 48.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (192.06, 86.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.121 CPU sec, 6.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13670, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 104, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (115.87, 57.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.74, 86.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.098 CPU sec, 7.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13644, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 139, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (147.05, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (192.60, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.580 CPU sec, 7.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13756, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 121, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (119.37, 65.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.32, 87.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.086 CPU sec, 5.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13768, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 131, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (127.09, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.74, 84.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.027 CPU sec, 5.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13689, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 95, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (97.08, 60.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (188.47, 83.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.335 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13743, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (149.73, 91.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (188.21, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.200 CPU sec, 5.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13747, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 129, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (142.67, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 169, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (189.69, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.436 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13649, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 105, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (112.00, 50.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (186.80, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.925 CPU sec, 5.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13424, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 104, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (113.16, 54.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 168, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (187.05, 82.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.292 CPU sec, 5.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13385, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 106, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (100.94, 63.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (128, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (187.47, 81.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.242 CPU sec, 6.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13444, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 107, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (113.48, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (190.53, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.396 CPU sec, 7.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13753, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 78, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (102.94, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (192.02, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.248 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13677, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 94, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (122.46, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (191.78, 86.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.259 CPU sec, 6.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13824, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 141, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (142.28, 87.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.50, 85.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.204 CPU sec, 5.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13690, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 86, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (122.00, 74.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.18, 85.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.036 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13780, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (129.20, 60.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (190.49, 85.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.567 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13564, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 153, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (132.31, 78.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.93, 88.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.052 CPU sec, 6.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13666, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 100, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (116.68, 62.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.17, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.434 CPU sec, 6.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13614, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (128.61, 65.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 173, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (193.97, 88.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.630 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13777, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (152.43, 85.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 172, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (192.89, 87.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.258 CPU sec, 6.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13840, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 93, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (149.69, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (193.10, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.423 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13667, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (137.45, 57.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (193.36, 87.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.500 CPU sec, 5.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13572, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (139.52, 81.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.88, 85.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.877 CPU sec, 6.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13632, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 143, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (139.11, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (129, 171, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (190.70, 86.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.003 CPU sec, 5.178 real sec [M::process] read 3510 sequences (530010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13803, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 115, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (157.39, 108.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (130, 171, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (194.25, 88.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.861 CPU sec, 6.023 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 748, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (197.26, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 3510 reads in 8.155 CPU sec, 0.509 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21185\tSM:UG10S2P535\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21185_HLKNYBGXX_UG10S2P535_R1.fastq.gz 5347_7332_21185_HLKNYBGXX_UG10S2P535_R2.fastq.gz [main] Real time: 3217.752 sec; CPU: 80185.833 sec Finished alignement of UG10S2P535 at Wed Apr 3 20:17:40 2019 Sorting BAM file started at Wed Apr 3 20:17:40 2019 samtools sort -@ 30 -m 4G UG10S2P535_unsorted.bam -o UG10S2P535.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 20:17:40 2019 rm UG10S2P535_unsorted.bam rm 5347_7332_21185_HLKNYBGXX_UG10S2P535_R1.fastq.gz rm 5347_7332_21185_HLKNYBGXX_UG10S2P535_R2.fastq.gz samtools index UG10S2P535.bam Finished BAM file generation of the sample - UG10S2P535 Processing fastq files for the sample - UG10S2P555 Reading 5347_7332_21186_HLKNYBGXX_UG10S2P555_R1.fastq.gz 5347_7332_21186_HLKNYBGXX_UG10S2P555_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21186\tSM:UG10S2P555\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21186_HLKNYBGXX_UG10S2P555_R1.fastq.gz 5347_7332_21186_HLKNYBGXX_UG10S2P555_R2.fastq.gz | samtools view -Sb1 - > UG10S2P555_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13557, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 160, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (160.64, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.10, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.846 CPU sec, 5.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13716, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 155, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (120.27, 59.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.90, 74.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.207 CPU sec, 6.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13722, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 107, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (140.77, 91.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.42, 76.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.887 CPU sec, 4.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13915, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 132, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (165.18, 110.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (167.46, 78.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.327 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13788, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 121, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (137.94, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.80, 76.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.031 CPU sec, 4.652 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13964, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 88, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (113.44, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.67, 78.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.576 CPU sec, 4.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13916, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 76, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (85.00, 43.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (168.09, 78.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.163 CPU sec, 4.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13983, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 107, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (125.50, 84.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (167.40, 78.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.104 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13945, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 166, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (177.93, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.10, 78.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.175 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13931, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 181, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (185.77, 143.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.12, 77.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.260 CPU sec, 5.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14186, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 155, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (157.53, 101.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.94, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.417 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13926, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (136.67, 87.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (165.88, 77.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.578 CPU sec, 4.958 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13959, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 107, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (131.76, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.61, 75.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.347 CPU sec, 5.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13982, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 217, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (185.08, 84.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.48, 76.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.192 CPU sec, 4.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14055, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 150, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (142.95, 78.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.14, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.980 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14014, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 170, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (140.41, 68.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.58, 76.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.043 CPU sec, 15.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14057, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 119, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (180.56, 148.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.26, 73.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.401 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13877, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 81, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (89.61, 35.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.30, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.583 CPU sec, 5.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13935, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 149, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (159.56, 106.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (163.72, 73.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.900 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13801, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 154, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (160.80, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.44, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.884 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13529, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 154, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (155.87, 87.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.50, 76.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.895 CPU sec, 4.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13621, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (149.75, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.75, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.590 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13661, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 154, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (156.23, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.44, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.102 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13882, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 125, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (110.50, 54.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.73, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.768 CPU sec, 4.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13821, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 101, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (122.64, 66.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.58, 77.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.495 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13874, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 136, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (161.83, 106.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (168.88, 78.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.990 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13952, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 217, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (203.00, 89.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.94, 77.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.869 CPU sec, 6.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13963, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (133.18, 62.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.42, 76.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.294 CPU sec, 4.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13961, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (121.38, 77.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (166.84, 77.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.623 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13881, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (117.78, 49.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.92, 78.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.577 CPU sec, 6.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13974, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 165, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (179.07, 105.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.39, 77.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.987 CPU sec, 4.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13962, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 85, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (122.33, 76.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.48, 78.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.500 CPU sec, 4.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13993, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 99, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (106.68, 56.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.79, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.924 CPU sec, 5.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13959, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.11, 77.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.472 CPU sec, 4.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14007, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 166, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (138.79, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.76, 76.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.745 CPU sec, 5.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13927, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (135.65, 104.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.19, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.025 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13756, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (134.27, 66.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (164.94, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.740 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13764, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (168.19, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.04, 74.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.351 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13873, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 155, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (156.93, 63.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.66, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.484 CPU sec, 5.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13800, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 151, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (137.79, 83.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.66, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.218 CPU sec, 5.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13603, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 122, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (136.64, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (165.08, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.309 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13885, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 100, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (135.08, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.56, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.001 CPU sec, 4.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13814, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 154, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (177.07, 126.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.89, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.708 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13809, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 118, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (126.44, 76.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.23, 77.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.201 CPU sec, 4.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13847, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 98, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (94.94, 42.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.20, 76.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.847 CPU sec, 4.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13980, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 134, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (128.93, 63.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.36, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.978 CPU sec, 3.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13880, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 129, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (124.08, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (168.52, 78.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.141 CPU sec, 3.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13841, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 142, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (190.00, 102.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (164.71, 76.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.712 CPU sec, 5.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13901, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (126.22, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.40, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.399 CPU sec, 4.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 14056, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (133.33, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.16, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.796 CPU sec, 4.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13785, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 139, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (126.95, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (164.76, 75.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.169 CPU sec, 4.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13889, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 97, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (100.08, 46.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (166.22, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.064 CPU sec, 4.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13864, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 83, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (69.86, 28.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.04, 76.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.275 CPU sec, 4.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13819, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 114, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (132.75, 83.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (165.57, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.617 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13812, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 106, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (110.35, 65.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.49, 76.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.571 CPU sec, 5.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13762, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 69, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (144.20, 118.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.79, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.015 CPU sec, 4.503 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13726, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 136, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (172.73, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.03, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.925 CPU sec, 5.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13985, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 147, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (129.95, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.54, 74.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.708 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13863, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 100, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (121.30, 68.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.29, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.989 CPU sec, 5.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13551, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 114, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (136.57, 77.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.50, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.024 CPU sec, 4.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13624, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (128.11, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (160.87, 70.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.276 CPU sec, 5.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13825, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (120.53, 79.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.50, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.680 CPU sec, 5.022 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13929, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 108, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (128.00, 79.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.94, 74.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.382 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13963, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 112, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (152.18, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.35, 76.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.841 CPU sec, 5.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14011, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 100, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (123.33, 79.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.67, 78.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.525 CPU sec, 4.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13950, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (155.46, 88.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.83, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.151 CPU sec, 4.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14014, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 106, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (127.05, 75.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.01, 76.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.360 CPU sec, 4.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13829, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 103, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (98.17, 40.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (166.12, 77.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.055 CPU sec, 6.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13744, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (117.80, 49.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.37, 75.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.888 CPU sec, 5.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14068, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 116, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (139.41, 95.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.39, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.385 CPU sec, 6.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13808, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 165, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (126.20, 81.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (166.86, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.389 CPU sec, 5.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13903, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 114, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (160.06, 94.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (167.50, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.794 CPU sec, 4.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13931, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (124.67, 64.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.90, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.928 CPU sec, 4.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13898, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 101, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (104.25, 63.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (161.96, 71.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.628 CPU sec, 5.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13972, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 89, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (127.67, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (164.91, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.622 CPU sec, 4.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13977, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 120, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (122.54, 67.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.64, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.154 CPU sec, 5.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13704, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 204, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (189.60, 79.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (164.44, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.500 CPU sec, 4.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13759, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 91, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (125.52, 94.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.21, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.821 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13986, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 95, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (105.76, 53.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.70, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.775 CPU sec, 6.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13648, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 117, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (126.84, 61.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.05, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.425 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13683, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 90, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (90.73, 55.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (161.98, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.822 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13731, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 170, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (181.25, 114.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.25, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.234 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13752, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.33, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.279 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13895, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 86, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (91.94, 39.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.73, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.831 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13870, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 121, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (140.95, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.35, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.748 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13851, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 135, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (134.53, 36.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.18, 74.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.769 CPU sec, 4.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13851, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 90, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (116.54, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.50, 74.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.111 CPU sec, 4.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13977, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 118, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (139.35, 87.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.46, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.270 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13799, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 106, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (129.35, 87.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.38, 75.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.216 CPU sec, 4.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13899, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 96, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (109.86, 83.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.73, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.835 CPU sec, 5.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13860, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 141, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (182.46, 109.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.79, 77.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.399 CPU sec, 4.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13799, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (133.24, 63.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (168.57, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.240 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13974, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 208, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (181.57, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.63, 76.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.676 CPU sec, 6.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13926, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 129, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (112.53, 56.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.82, 76.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.268 CPU sec, 5.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13878, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 86, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 162) [M::mem_pestat] mean and std.dev: (77.43, 27.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.24, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.360 CPU sec, 4.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13913, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 83, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (125.21, 90.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.03, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.756 CPU sec, 4.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13811, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 157, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (162.81, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.67, 74.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.959 CPU sec, 5.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13848, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 183, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (151.64, 113.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.13, 74.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.758 CPU sec, 4.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13926, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 142, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (161.38, 120.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.38, 73.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.427 CPU sec, 4.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13497, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 141, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (149.12, 70.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.93, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.249 CPU sec, 4.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13735, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 120, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (133.50, 59.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.91, 72.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.647 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13810, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 91, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (100.09, 52.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.65, 72.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.523 CPU sec, 4.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13943, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 182, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (181.89, 50.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (161.89, 72.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.213 CPU sec, 4.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13936, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 122, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (124.41, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.65, 74.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.631 CPU sec, 6.012 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13826, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (43, 115, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (113.25, 87.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.97, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.807 CPU sec, 4.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13816, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.80, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 130.041 CPU sec, 5.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13701, 9, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 101, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (107.93, 44.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.02, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.289 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13856, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (123.00, 87.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.16, 76.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.836 CPU sec, 4.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13958, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 100, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (99.88, 46.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.75, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.013 CPU sec, 5.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13721, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 146, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (158.89, 89.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.06, 74.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.790 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13857, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 102, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (114.75, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.77, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.013 CPU sec, 5.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13771, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 138, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (128.67, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.15, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.908 CPU sec, 4.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13916, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 119, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.69, 92.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.85, 76.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.274 CPU sec, 4.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13962, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (108.16, 54.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.58, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.604 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13858, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (124.50, 73.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (163.22, 73.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.424 CPU sec, 4.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13733, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 100, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (105.05, 50.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (162.92, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.755 CPU sec, 4.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13796, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 159, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (174.33, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.49, 75.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.220 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13662, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (155.24, 91.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.79, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.257 CPU sec, 4.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13853, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (131.95, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.89, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.796 CPU sec, 4.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13692, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (166.93, 77.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.310 CPU sec, 5.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13834, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 100, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (121.25, 83.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.88, 79.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.158 CPU sec, 5.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13756, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 115, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (132.69, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (168.01, 78.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.049 CPU sec, 4.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 14042, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 198, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (171.25, 99.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (166.55, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.294 CPU sec, 4.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13904, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 85, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (134.93, 99.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (167.37, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.241 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13978, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (126.05, 67.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.82, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.419 CPU sec, 4.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13996, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 132, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (172.71, 113.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.80, 77.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.480 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13914, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.56, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.965 CPU sec, 4.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13881, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 111, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (173.79, 131.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.03, 77.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.887 CPU sec, 4.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13756, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 109, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (151.85, 99.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (164.94, 76.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.790 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13903, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 95, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (96.45, 53.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (164.89, 75.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.490 CPU sec, 4.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14029, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 93, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (150.11, 127.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.04, 77.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.838 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13929, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 96, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (102.06, 57.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.49, 77.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.466 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 14092, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 90, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (113.24, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.69, 78.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.864 CPU sec, 5.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13801, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 120, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (136.67, 91.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.71, 74.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.516 CPU sec, 4.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13883, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 161, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (152.70, 81.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.33, 73.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.173 CPU sec, 4.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13711, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (134.35, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (162.15, 72.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.136 CPU sec, 5.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13663, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 186, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (177.88, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (162.09, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.559 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13512, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 86, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (120.43, 81.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (160.73, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.262 CPU sec, 5.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13441, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 109, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (114.75, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.98, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.050 CPU sec, 5.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13790, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 216, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (221.62, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (169.20, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.763 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13867, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 83, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (130.58, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (165.84, 76.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.644 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13819, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 158, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (203.19, 153.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (165.85, 76.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.645 CPU sec, 4.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13830, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 165, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (181.29, 86.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (168.99, 78.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.567 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13780, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (83.22, 41.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (169.91, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.092 CPU sec, 5.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13758, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 117, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (152.50, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (168.49, 78.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.063 CPU sec, 5.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14055, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 136, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (182.19, 152.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (168.83, 79.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.378 CPU sec, 4.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13649, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 165, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (181.36, 94.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.02, 77.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.249 CPU sec, 4.751 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 13775, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (168.26, 79.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 141.468 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13794, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 111, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (119.93, 50.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (169.28, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.909 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13872, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 123, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (122.25, 65.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.28, 77.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.631 CPU sec, 4.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13825, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 235, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (201.41, 104.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.77, 78.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.945 CPU sec, 4.572 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13897, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (135.37, 88.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.00, 77.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.068 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13945, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (128.36, 63.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (165.52, 76.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.879 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13767, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 113, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (107.60, 51.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.75, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.659 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13913, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 125, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (131.30, 78.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.06, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.973 CPU sec, 4.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13744, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 118, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (113.43, 50.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.58, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.903 CPU sec, 4.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13664, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (139.95, 75.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.89, 72.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.274 CPU sec, 4.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13448, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (141.85, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (161.32, 71.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.681 CPU sec, 4.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13491, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (114.71, 47.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (165.89, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.229 CPU sec, 5.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13866, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 105, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (103.47, 54.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (168.35, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.475 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13848, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.11, 77.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 125.380 CPU sec, 6.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13966, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 115, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (115.85, 49.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (169.20, 80.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.388 CPU sec, 4.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13945, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 97, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (90.67, 60.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (168.75, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.389 CPU sec, 5.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14064, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (129.45, 61.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.11, 77.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.380 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13879, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (31, 95, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (100.19, 72.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.42, 78.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.843 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13957, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 122, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (133.07, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (169.07, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.668 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13948, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 161, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (149.46, 80.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.21, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.232 CPU sec, 4.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13836, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (121.32, 71.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.35, 76.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.311 CPU sec, 4.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14009, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 144, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (165.44, 105.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.23, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.310 CPU sec, 6.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13989, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (135.50, 97.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.85, 75.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.241 CPU sec, 4.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13590, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 140, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (156.82, 113.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.14, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.232 CPU sec, 5.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13872, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 86, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (103.44, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.74, 75.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.520 CPU sec, 4.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13915, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 143, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (127.21, 61.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (162.66, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.787 CPU sec, 5.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13913, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (162.97, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.952 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13703, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 96, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (115.86, 60.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.04, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.954 CPU sec, 5.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13605, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (161.77, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 138.540 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13414, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 118, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 250) [M::mem_pestat] mean and std.dev: (117.45, 29.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (161.97, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.810 CPU sec, 5.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13509, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 129, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (138.20, 98.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (159.96, 69.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.535 CPU sec, 5.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13672, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 135, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (121.36, 60.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.69, 75.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.577 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13870, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319) [M::mem_pestat] mean and std.dev: (111.07, 53.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (166.20, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.751 CPU sec, 5.919 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13855, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 127, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (170.13, 112.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.71, 77.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.569 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13950, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 77, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (110.44, 83.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.36, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.078 CPU sec, 4.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13867, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (185.80, 85.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.72, 75.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.324 CPU sec, 5.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13918, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (127.06, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.35, 74.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.532 CPU sec, 4.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13948, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 90, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (120.46, 87.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.39, 77.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.720 CPU sec, 4.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13908, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (145.64, 93.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.70, 75.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.491 CPU sec, 4.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13691, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 84, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (122.17, 111.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.03, 75.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.367 CPU sec, 4.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13804, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (129.64, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.10, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.179 CPU sec, 4.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14057, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 102, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (108.57, 58.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.97, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.985 CPU sec, 4.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13745, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 160, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (189.05, 128.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.45, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.914 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13896, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (121.00, 83.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.36, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.048 CPU sec, 4.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13907, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (150.88, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.92, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.192 CPU sec, 5.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13951, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 149, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (154.43, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.58, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.815 CPU sec, 4.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13748, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 113, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (120.06, 56.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.38, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.762 CPU sec, 4.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13664, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 72, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (88.94, 44.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (162.43, 72.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.895 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13678, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (149.33, 87.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (161.28, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.640 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13425, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 116, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (136.10, 87.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (160.00, 69.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.652 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13419, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 123, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (120.83, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (159.68, 69.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.582 CPU sec, 4.421 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13716, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 105, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (108.64, 58.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.45, 77.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.325 CPU sec, 4.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13629, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 77, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (89.83, 54.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (165.93, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.556 CPU sec, 4.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13910, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 103, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (103.42, 63.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (165.69, 75.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.200 CPU sec, 4.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13773, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (112.44, 76.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.82, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.607 CPU sec, 4.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13739, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 126, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (148.27, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.27, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.860 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13789, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 95, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (134.24, 92.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.40, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.410 CPU sec, 4.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13797, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (129.50, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (168.84, 79.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.047 CPU sec, 4.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13841, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (152.17, 126.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.14, 76.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.642 CPU sec, 4.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13804, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 136, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (137.57, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.72, 77.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.872 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13931, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 122, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (128.37, 64.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.39, 77.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.492 CPU sec, 4.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13948, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 105, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (122.85, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.70, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.831 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14002, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 107, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (109.57, 54.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.99, 75.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.745 CPU sec, 4.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13856, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 116, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (145.21, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.88, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.178 CPU sec, 4.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13782, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 103, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (118.84, 69.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (167.07, 77.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.638 CPU sec, 6.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13820, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 107, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (115.12, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.39, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.818 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13894, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (131.43, 78.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.76, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.830 CPU sec, 4.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13888, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (135.27, 81.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (161.00, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.484 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13603, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 131, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (138.24, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (163.08, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.649 CPU sec, 5.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13431, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 116, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (141.00, 73.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (159.53, 69.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.975 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13585, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 115, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (120.24, 84.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (162.50, 71.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.502 CPU sec, 5.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13733, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 141, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (142.94, 74.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.43, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.951 CPU sec, 4.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13644, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 78, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (109.89, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.26, 76.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.206 CPU sec, 4.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13802, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 186, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (171.75, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (165.12, 74.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.026 CPU sec, 5.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13908, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 160, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (161.93, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (168.11, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.195 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13787, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 97, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (117.23, 59.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.47, 74.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.784 CPU sec, 5.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13924, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 115, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (95.92, 57.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.47, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.993 CPU sec, 5.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13885, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 64, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 114) [M::mem_pestat] mean and std.dev: (65.56, 17.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.94, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.520 CPU sec, 4.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13698, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 99, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (87.23, 41.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.33, 76.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.429 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13818, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 87, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (79.50, 37.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.81, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.851 CPU sec, 4.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13942, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 123, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (111.00, 44.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.92, 75.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.406 CPU sec, 4.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13954, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 141, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (169.13, 145.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.17, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.931 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13819, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 153, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (161.46, 91.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.65, 74.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.565 CPU sec, 4.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13789, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 146, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (156.18, 85.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (165.09, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.551 CPU sec, 5.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13964, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 102, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (102.64, 51.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.20, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.678 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13698, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 111, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (118.50, 45.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (162.31, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.063 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13743, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 117, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (155.53, 94.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.85, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.783 CPU sec, 4.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13798, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 95, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (140.74, 105.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (162.21, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.810 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13713, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 74, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (93.08, 66.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (158.25, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.973 CPU sec, 4.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13227, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (130.38, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (157.78, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.954 CPU sec, 5.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13557, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 113, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (137.17, 105.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.21, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.292 CPU sec, 5.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13887, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 131, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (117.06, 52.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (161.43, 71.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.942 CPU sec, 4.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13930, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (109.50, 70.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.36, 74.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.810 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 14073, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 130, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (124.75, 56.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.33, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.459 CPU sec, 4.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13947, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 106, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (112.38, 58.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.42, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.042 CPU sec, 4.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13910, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 79, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (101.00, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.89, 74.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.886 CPU sec, 4.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13816, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 81, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (152.29, 123.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.90, 75.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.035 CPU sec, 6.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14038, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 124, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (116.18, 49.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (164.84, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.347 CPU sec, 4.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14072, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 121, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (139.38, 85.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.53, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.711 CPU sec, 4.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13982, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 141, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (141.80, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.30, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.884 CPU sec, 4.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14015, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 90, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (142.76, 103.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.73, 76.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.627 CPU sec, 4.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13970, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 104, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (88.62, 44.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.64, 76.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.104 CPU sec, 4.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13812, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 81, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (87.71, 55.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (162.94, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.352 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13822, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 84, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (118.69, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.24, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.453 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13858, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 104, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (154.29, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.44, 75.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.829 CPU sec, 4.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13856, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 103, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (119.95, 56.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.05, 75.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.031 CPU sec, 4.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13869, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 121, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (132.53, 69.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.16, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.307 CPU sec, 4.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13781, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 154, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (128.50, 59.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.57, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.364 CPU sec, 4.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13770, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 136, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (133.11, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (167.67, 77.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.841 CPU sec, 4.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13901, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 156, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (130.75, 65.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.58, 77.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.909 CPU sec, 4.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13831, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 138, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (165.94, 117.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.82, 74.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.604 CPU sec, 5.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13996, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 132, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (141.21, 93.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.79, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.825 CPU sec, 4.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13764, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 97, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (112.35, 77.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.04, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.192 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13944, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 127, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (144.20, 87.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.20, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.359 CPU sec, 4.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13800, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 98, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (91.75, 39.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.41, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.396 CPU sec, 4.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13968, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (126.00, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (166.09, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.294 CPU sec, 5.456 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14044, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 122, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (140.31, 90.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.32, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.095 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13987, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 148, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (142.27, 56.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.78, 76.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.853 CPU sec, 5.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13933, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 96, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (102.55, 54.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.38, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.372 CPU sec, 4.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13995, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 111, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (132.00, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (167.96, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.400 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14048, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 88, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (94.21, 59.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.72, 77.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.117 CPU sec, 4.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13956, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (213.69, 185.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.07, 78.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.866 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13658, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 109, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (111.50, 50.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.56, 76.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.528 CPU sec, 5.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13646, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 161, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (165.80, 114.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.84, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.691 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13793, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 190, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (171.85, 94.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.06, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.006 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13931, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.80, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.593 CPU sec, 5.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13836, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 110, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 209) [M::mem_pestat] mean and std.dev: (97.67, 33.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.79, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.277 CPU sec, 4.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13850, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 117, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (103.50, 52.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (167.01, 76.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.616 CPU sec, 4.483 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14032, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 120, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (145.29, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.83, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.647 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13810, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 103, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (139.33, 89.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (165.01, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.103 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13892, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 81, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (122.50, 98.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.13, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.312 CPU sec, 4.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13708, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 67, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (111.67, 76.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.69, 73.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.186 CPU sec, 4.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14008, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 170, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (166.53, 110.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.61, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.683 CPU sec, 4.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13795, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 110, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (108.27, 42.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.07, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.232 CPU sec, 4.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13958, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 138, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (169.56, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.01, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.144 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13958, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 163, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (131.56, 74.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (166.66, 77.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.127 CPU sec, 4.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13890, 7, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.89, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.928 CPU sec, 5.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13965, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 150, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (141.44, 62.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.83, 77.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.685 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13945, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (146.75, 81.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.33, 76.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.428 CPU sec, 5.089 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13910, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 85, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (111.00, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.05, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.751 CPU sec, 4.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14019, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (164.14, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.26, 74.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.142 CPU sec, 4.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13709, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (165.42, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.205 CPU sec, 4.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13683, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 220, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (168.27, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (163.25, 72.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.061 CPU sec, 5.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13764, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 98, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (152.86, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.43, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.519 CPU sec, 5.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13811, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (117.12, 62.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.52, 72.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.909 CPU sec, 4.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13965, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 99, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (100.85, 56.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (165.82, 76.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.456 CPU sec, 4.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13801, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (33, 157, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (141.18, 100.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (166.68, 77.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.609 CPU sec, 4.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13850, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (133.40, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (167.93, 79.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.200 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13791, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (107.86, 50.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.61, 75.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.874 CPU sec, 4.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14036, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 132, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (129.86, 80.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.90, 74.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.130 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14062, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 95, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (122.68, 89.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.45, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.153 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13884, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (105.56, 55.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.31, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.902 CPU sec, 4.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13729, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (139.62, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.16, 73.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.346 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13807, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (127.71, 78.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.55, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.089 CPU sec, 5.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13739, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 184, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (161.29, 89.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (163.69, 74.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.792 CPU sec, 5.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13899, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (147.57, 78.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.77, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.942 CPU sec, 5.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13811, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 97, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (98.85, 47.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.34, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.856 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13935, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 103, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (149.56, 119.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.35, 73.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.411 CPU sec, 5.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13834, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 114, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (131.43, 61.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (162.16, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.474 CPU sec, 4.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13790, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 84, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (86.59, 54.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.25, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.812 CPU sec, 4.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13974, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.23, 73.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 131.268 CPU sec, 5.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13645, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 166, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (186.29, 122.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (162.55, 73.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.723 CPU sec, 4.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13895, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 170, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (179.45, 100.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (159.76, 70.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.258 CPU sec, 4.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13665, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 94, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (106.88, 62.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.55, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.362 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13848, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 128, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (118.88, 58.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.47, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.155 CPU sec, 4.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13706, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 88, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (73.15, 25.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.33, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.906 CPU sec, 4.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13744, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 105, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (97.09, 46.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.82, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.576 CPU sec, 4.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13915, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 168, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (175.81, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.42, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.132 CPU sec, 4.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13974, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (44, 76, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (111.89, 92.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (165.39, 77.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.586 CPU sec, 4.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14037, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 117, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (133.35, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.10, 75.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.394 CPU sec, 4.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13825, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 161, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (150.14, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.81, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.327 CPU sec, 4.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13933, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 128, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (116.00, 47.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.61, 73.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.177 CPU sec, 4.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14072, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (131.38, 69.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.35, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.063 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13827, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (163.23, 104.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.50, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.947 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13784, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (142.00, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.41, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.186 CPU sec, 4.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13904, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 193) [M::mem_pestat] mean and std.dev: (96.17, 37.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.73, 74.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.698 CPU sec, 4.801 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13794, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 170, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (135.85, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.73, 76.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.725 CPU sec, 5.796 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14135, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 131, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (120.79, 58.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.39, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.456 CPU sec, 4.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13921, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 138, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (158.00, 95.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (164.29, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.060 CPU sec, 5.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13873, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 85, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (110.88, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (162.06, 72.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.122 CPU sec, 4.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13673, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (129.30, 48.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (160.27, 69.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.717 CPU sec, 4.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13753, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 130, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (151.94, 98.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (161.34, 71.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.641 CPU sec, 4.535 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13787, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 102, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (125.24, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.31, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.824 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13829, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.83, 72.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.536 CPU sec, 4.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13722, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 88, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (115.07, 76.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.44, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.534 CPU sec, 4.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13841, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 105, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (130.06, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.76, 73.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.106 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13923, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 74, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (123.31, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.49, 74.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.098 CPU sec, 6.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13810, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (109.67, 60.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.60, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.057 CPU sec, 4.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 14037, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 102, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (125.82, 86.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (162.34, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.591 CPU sec, 5.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13905, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 87, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (95.70, 50.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.56, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.310 CPU sec, 6.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13935, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 122, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (123.38, 57.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.44, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.467 CPU sec, 5.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13908, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 77, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (101.27, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.97, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.641 CPU sec, 4.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13897, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 93, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (116.40, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (163.52, 74.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.598 CPU sec, 4.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 14067, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (109.62, 59.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.71, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.526 CPU sec, 4.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13962, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (134.62, 81.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.52, 74.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.824 CPU sec, 5.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13948, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 123, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (179.09, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (163.14, 72.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.817 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13934, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.14, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.230 CPU sec, 5.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13689, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 84, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (103.29, 68.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (166.23, 76.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.870 CPU sec, 4.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13858, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 90, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (84.14, 36.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.76, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.847 CPU sec, 4.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13821, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 161, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (138.07, 68.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.33, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.859 CPU sec, 5.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13809, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 99, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (129.54, 61.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (161.85, 71.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.289 CPU sec, 4.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13871, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 105, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (102.06, 46.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (161.16, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.180 CPU sec, 4.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13819, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 99, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (127.37, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (164.11, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.204 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13831, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 119, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (94.81, 40.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.89, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.056 CPU sec, 5.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13930, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (126.09, 94.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.35, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.511 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13979, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 113, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (161.94, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.53, 76.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.034 CPU sec, 4.834 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13788, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 87, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (121.33, 96.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.22, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.061 CPU sec, 4.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13978, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 98, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (119.76, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (162.96, 72.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.925 CPU sec, 4.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13647, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (145.45, 92.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.49, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.219 CPU sec, 5.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13594, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 186, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (148.62, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (162.33, 71.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.560 CPU sec, 4.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13599, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 157, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (186.77, 113.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (161.42, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.025 CPU sec, 6.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13518, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 122, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (170.27, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (160.10, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.099 CPU sec, 5.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13597, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 108, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (111.16, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (165.75, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.200 CPU sec, 5.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13925, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 132, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (153.41, 117.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (163.69, 74.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.286 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 14003, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 207, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (172.90, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (164.60, 75.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.393 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14017, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 128, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (121.27, 49.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.04, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.712 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14071, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 172, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (152.69, 55.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.13, 76.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.388 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13719, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (185.33, 101.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.54, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.666 CPU sec, 5.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13738, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 134, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (158.27, 87.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.47, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.981 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13822, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 129, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (179.00, 119.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.07, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.459 CPU sec, 4.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13853, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 90, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (124.82, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.58, 76.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.907 CPU sec, 4.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13930, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 151, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (190.65, 132.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.87, 76.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.602 CPU sec, 4.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13896, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 138, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (155.78, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (167.90, 78.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.607 CPU sec, 5.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13923, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 96, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (121.58, 74.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.06, 76.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.356 CPU sec, 4.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13835, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 83, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (110.36, 64.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.56, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.253 CPU sec, 4.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13905, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 114, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (107.23, 47.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.31, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.634 CPU sec, 4.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13796, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 165, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (187.69, 111.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (162.38, 72.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.602 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13818, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.54, 76.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 135.563 CPU sec, 5.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13949, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 107, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (142.56, 91.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.23, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.730 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13563, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 142, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (137.87, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (161.07, 70.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.804 CPU sec, 6.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13543, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 74, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (91.17, 65.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (161.82, 70.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.557 CPU sec, 4.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13564, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 104, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (96.59, 39.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.92, 73.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.401 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13803, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 100, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (107.31, 63.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (168.47, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.968 CPU sec, 5.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13852, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 142, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (173.48, 118.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.20, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.682 CPU sec, 5.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13862, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 94, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 242) [M::mem_pestat] mean and std.dev: (106.88, 53.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.61, 74.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.165 CPU sec, 4.827 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13970, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 116, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (135.33, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (167.45, 77.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.247 CPU sec, 4.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13973, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 130, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (136.39, 69.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (166.88, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.932 CPU sec, 4.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13901, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (130.00, 81.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.72, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.475 CPU sec, 4.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13913, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 115, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (142.50, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.67, 78.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.030 CPU sec, 4.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13761, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 113, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 228) [M::mem_pestat] mean and std.dev: (100.75, 37.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (168.02, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.551 CPU sec, 5.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 14097, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 78, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (106.71, 72.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (166.59, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.822 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13874, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 133, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (147.36, 96.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (168.32, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.449 CPU sec, 4.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13823, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 138, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (128.94, 75.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (168.25, 78.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.134 CPU sec, 4.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13926, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 159, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (166.80, 62.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.43, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.350 CPU sec, 4.063 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13948, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 148, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (134.80, 61.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.72, 76.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.847 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13812, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 133, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (128.92, 46.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.55, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.298 CPU sec, 4.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13883, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (128.92, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.40, 72.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.125 CPU sec, 5.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13693, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 84, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (101.00, 59.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.03, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.863 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13606, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 114, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (124.39, 68.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (161.51, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.658 CPU sec, 7.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13719, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 130, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (151.29, 55.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (160.70, 70.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.242 CPU sec, 5.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13359, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 110, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (106.00, 43.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.61, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.361 CPU sec, 5.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13872, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 151, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (147.85, 91.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.23, 75.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.619 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13898, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 81, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (115.38, 69.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (167.76, 78.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.657 CPU sec, 5.374 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 14048, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 134, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (144.70, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.05, 73.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.903 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 14070, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 78, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (105.38, 53.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.12, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.647 CPU sec, 4.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 14030, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 98, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (132.55, 108.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.59, 78.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.215 CPU sec, 5.116 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13834, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 152, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (164.93, 93.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.90, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.248 CPU sec, 5.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13980, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 165, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (210.05, 145.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.42, 77.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.945 CPU sec, 4.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13992, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 124, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (131.47, 79.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.67, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.392 CPU sec, 4.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13926, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (107.60, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.68, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.872 CPU sec, 5.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13909, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 147, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (167.85, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.81, 79.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.997 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13891, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 84, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (111.20, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.71, 76.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.861 CPU sec, 4.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 14065, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 106, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (87.73, 55.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (168.47, 79.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.359 CPU sec, 4.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13964, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 79, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (105.67, 64.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.66, 77.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.095 CPU sec, 5.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14027, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (130.00, 84.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (161.87, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.622 CPU sec, 4.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13940, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 137, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (128.45, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.40, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.627 CPU sec, 4.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13686, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 158, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (144.30, 92.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (161.49, 71.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.058 CPU sec, 4.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13631, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (146.57, 58.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (159.39, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.788 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13546, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 103, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (129.06, 57.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.37, 67.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.119 CPU sec, 4.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13623, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (114.54, 57.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (157.52, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.359 CPU sec, 5.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13815, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 160, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (154.19, 59.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (164.05, 73.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.939 CPU sec, 4.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13792, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (111.84, 68.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.78, 74.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.142 CPU sec, 4.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13895, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 165, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (150.79, 74.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.91, 73.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.420 CPU sec, 4.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 14026, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (139.67, 104.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.66, 75.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.300 CPU sec, 4.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13980, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 130, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 231) [M::mem_pestat] mean and std.dev: (131.09, 24.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.00, 75.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.175 CPU sec, 4.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13708, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (140.31, 67.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (162.89, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.543 CPU sec, 4.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13894, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 126, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (149.57, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.05, 74.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.168 CPU sec, 5.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13819, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 106, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (98.92, 49.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (164.71, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.476 CPU sec, 5.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13914, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 119, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (143.10, 100.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.49, 75.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.142 CPU sec, 5.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13779, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 109, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (115.94, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.07, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.008 CPU sec, 5.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13820, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 126, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (131.35, 68.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (164.78, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.902 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13840, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 118, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (128.75, 60.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.22, 76.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.042 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13740, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (119.92, 54.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (167.49, 77.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.860 CPU sec, 4.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13747, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (138.94, 74.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.49, 73.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.778 CPU sec, 4.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13913, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 102, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (123.80, 66.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (161.43, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.289 CPU sec, 4.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13787, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 93, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (101.79, 46.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (159.38, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.782 CPU sec, 3.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13720, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (153.44, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.22, 67.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.578 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13563, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 118, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (138.18, 89.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 144, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (158.97, 68.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.126 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13409, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 149, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (143.12, 54.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.55, 67.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.482 CPU sec, 5.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13855, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 96, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (121.69, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.99, 75.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.058 CPU sec, 5.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13938, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 98, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (131.75, 91.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.84, 79.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.788 CPU sec, 4.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13789, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 99, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (102.21, 49.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (166.34, 76.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.534 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13865, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 173, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (189.56, 134.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.07, 73.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.090 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 13851, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.18, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.751 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13893, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 161, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (170.60, 102.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.15, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.864 CPU sec, 4.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13995, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (120.11, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.38, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.889 CPU sec, 4.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13942, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 118, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (105.92, 45.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.91, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.603 CPU sec, 5.536 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13995, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 103, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (133.38, 97.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (164.28, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.743 CPU sec, 4.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13826, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (162.87, 73.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.713 CPU sec, 4.611 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14001, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 117, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (100.29, 53.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.89, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.635 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13843, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (121.10, 56.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (167.13, 77.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.147 CPU sec, 4.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13994, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 100, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (112.00, 66.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.37, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.454 CPU sec, 5.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13927, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 92, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (124.53, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (164.38, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.073 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13937, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (111.11, 74.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (161.70, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.353 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13918, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (119.61, 77.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.78, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.617 CPU sec, 3.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13764, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 103, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (105.12, 46.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (163.03, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.369 CPU sec, 3.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13905, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 145, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (159.74, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.093 CPU sec, 4.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13638, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (133.92, 77.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (161.96, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.863 CPU sec, 4.775 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13686, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 115, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (116.86, 84.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 144, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (160.21, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.879 CPU sec, 4.180 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13854, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 99, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (91.29, 46.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.97, 76.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.821 CPU sec, 4.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13896, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (99.85, 42.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.00, 75.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.756 CPU sec, 5.864 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13838, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (164.96, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 139.358 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 14119, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 135, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (118.07, 38.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (165.62, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.104 CPU sec, 4.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13685, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 102, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (108.65, 60.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 146, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (163.84, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.917 CPU sec, 4.854 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13973, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (31, 90, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (78.80, 53.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (162.81, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.112 CPU sec, 4.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13902, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 78, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (91.89, 39.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.92, 76.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.068 CPU sec, 4.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 14080, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (138.67, 71.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (166.33, 76.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.894 CPU sec, 4.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13908, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 88, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (106.42, 55.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.05, 72.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.329 CPU sec, 4.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 14092, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (132.84, 67.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.54, 75.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.965 CPU sec, 4.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13891, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 130, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (157.00, 105.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (166.89, 77.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.584 CPU sec, 5.278 real sec [M::process] read 25670 sequences (3876170 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13998, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (164.52, 75.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 109.229 CPU sec, 4.056 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 5396, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (165.02, 75.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 25670 reads in 42.734 CPU sec, 2.016 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21186\tSM:UG10S2P555\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21186_HLKNYBGXX_UG10S2P555_R1.fastq.gz 5347_7332_21186_HLKNYBGXX_UG10S2P555_R2.fastq.gz [main] Real time: 2318.742 sec; CPU: 58555.930 sec Finished alignement of UG10S2P555 at Wed Apr 3 21:14:27 2019 Sorting BAM file started at Wed Apr 3 21:14:27 2019 samtools sort -@ 30 -m 4G UG10S2P555_unsorted.bam -o UG10S2P555.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 21:14:27 2019 rm UG10S2P555_unsorted.bam rm 5347_7332_21186_HLKNYBGXX_UG10S2P555_R1.fastq.gz rm 5347_7332_21186_HLKNYBGXX_UG10S2P555_R2.fastq.gz samtools index UG10S2P555.bam Finished BAM file generation of the sample - UG10S2P555 Processing fastq files for the sample - UG10S1P047 Reading 5347_7332_21187_HLKNYBGXX_UG10S1P047_R1.fastq.gz 5347_7332_21187_HLKNYBGXX_UG10S1P047_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21187\tSM:UG10S1P047\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21187_HLKNYBGXX_UG10S1P047_R1.fastq.gz 5347_7332_21187_HLKNYBGXX_UG10S1P047_R2.fastq.gz | samtools view -Sb1 - > UG10S1P047_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13282, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 152, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (158.65, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (234.59, 119.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.570 CPU sec, 7.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13486, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (123.75, 64.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (228.84, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.605 CPU sec, 6.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13226, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 143, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (136.78, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.52, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.812 CPU sec, 6.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13442, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 119, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (175.68, 160.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (231.72, 116.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.839 CPU sec, 8.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13211, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 147, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (147.17, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (232.83, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.099 CPU sec, 6.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13164, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 156, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (153.19, 66.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (229.23, 115.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.631 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13323, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 154, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (205.71, 142.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (230.53, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.204 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13327, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 140, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (154.88, 98.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (225.86, 112.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.654 CPU sec, 5.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13204, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 171, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (167.44, 91.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.32, 114.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.314 CPU sec, 6.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13403, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 100, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (115.17, 54.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (226.25, 112.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.266 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13200, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (113.55, 62.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.13, 115.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.713 CPU sec, 5.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13160, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 152, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (170.04, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (227.10, 114.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.961 CPU sec, 6.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13548, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 183, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (180.20, 80.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (230.91, 117.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.414 CPU sec, 6.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13088, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (226.09, 113.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.062 CPU sec, 5.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13460, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (131.39, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (236.02, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.467 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13096, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 207, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (210.10, 131.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (226.52, 113.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.056 CPU sec, 7.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13482, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 122, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (136.70, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (235.36, 119.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.071 CPU sec, 5.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13038, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 204, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (196.94, 114.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (227.78, 115.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.421 CPU sec, 6.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13326, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 104, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (139.93, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (230.82, 116.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.904 CPU sec, 7.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13204, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 159, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (159.88, 73.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (223.96, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.736 CPU sec, 5.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13453, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 219, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (250.80, 143.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (230.88, 116.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.255 CPU sec, 6.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13015, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 149, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (187.42, 147.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (225.18, 112.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.578 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13278, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 136, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (120.62, 46.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.15, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.386 CPU sec, 5.504 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13024, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 281, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (251.42, 144.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (223.97, 110.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.031 CPU sec, 6.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13259, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (168.70, 88.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.55, 117.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.788 CPU sec, 5.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13067, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 147, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (149.91, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.75, 117.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.324 CPU sec, 7.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13465, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 86, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (100.91, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.11, 115.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.925 CPU sec, 5.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13177, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 188, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (185.21, 99.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (230.56, 116.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.187 CPU sec, 5.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13301, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 170, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (180.48, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (226.85, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.072 CPU sec, 5.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13202, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 152, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (159.76, 107.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (230.26, 116.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.027 CPU sec, 6.157 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13255, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 170, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (143.71, 76.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (227.42, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.192 CPU sec, 5.728 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13296, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 136, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (173.46, 105.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (224.20, 112.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.304 CPU sec, 6.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13316, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 160, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (180.62, 124.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (224.88, 112.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.232 CPU sec, 5.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13307, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 175, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (190.00, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.31, 110.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.881 CPU sec, 4.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13400, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 99, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (115.26, 76.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (225.65, 112.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.794 CPU sec, 6.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13050, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 125, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (151.50, 92.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (225.78, 113.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.263 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13365, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 92, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (157.00, 114.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.80, 117.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.288 CPU sec, 5.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13075, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 159, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (170.73, 113.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (224.49, 111.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.630 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13430, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 95, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (150.80, 104.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (232.15, 117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.073 CPU sec, 5.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12879, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (117.17, 62.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (226.30, 113.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.711 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13319, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 114, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (153.71, 107.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (232.06, 116.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.777 CPU sec, 6.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12877, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 154, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (127.12, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (221.74, 109.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.526 CPU sec, 5.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13270, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 202, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (184.67, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (228.82, 115.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.906 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13048, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 137, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (160.11, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (222.14, 109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.810 CPU sec, 5.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13364, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 145, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (150.16, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.82, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.279 CPU sec, 5.758 real sec [M::process] read 64026 sequences (9667926 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12955, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 104, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (86.94, 37.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (223.11, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.790 CPU sec, 5.604 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12897, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 125, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (126.45, 68.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (229.64, 115.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 64026 reads in 157.267 CPU sec, 6.560 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21187\tSM:UG10S1P047\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21187_HLKNYBGXX_UG10S1P047_R1.fastq.gz 5347_7332_21187_HLKNYBGXX_UG10S1P047_R2.fastq.gz [main] Real time: 289.062 sec; CPU: 7524.678 sec Finished alignement of UG10S1P047 at Wed Apr 3 21:55:54 2019 Sorting BAM file started at Wed Apr 3 21:55:54 2019 samtools sort -@ 30 -m 4G UG10S1P047_unsorted.bam -o UG10S1P047.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 21:55:54 2019 rm UG10S1P047_unsorted.bam rm 5347_7332_21187_HLKNYBGXX_UG10S1P047_R1.fastq.gz rm 5347_7332_21187_HLKNYBGXX_UG10S1P047_R2.fastq.gz samtools index UG10S1P047.bam Finished BAM file generation of the sample - UG10S1P047 Processing fastq files for the sample - UG10S2P655 Reading 5347_7332_21188_HLKNYBGXX_UG10S2P655_R1.fastq.gz 5347_7332_21188_HLKNYBGXX_UG10S2P655_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21188\tSM:UG10S2P655\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21188_HLKNYBGXX_UG10S2P655_R1.fastq.gz 5347_7332_21188_HLKNYBGXX_UG10S2P655_R2.fastq.gz | samtools view -Sb1 - > UG10S2P655_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4977, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 260, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (281.90, 142.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1070.714 CPU sec, 37.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4954, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 271, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (291.24, 144.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1116.936 CPU sec, 37.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 4776, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 275, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (289.24, 144.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1161.777 CPU sec, 38.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4680, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 267, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (284.93, 144.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1122.705 CPU sec, 37.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4719, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 253, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (277.31, 140.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1069.736 CPU sec, 36.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4912, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 268, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (286.63, 142.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1051.356 CPU sec, 35.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4916, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 269, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (289.91, 144.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1096.025 CPU sec, 36.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4912, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 272, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (289.63, 144.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1120.600 CPU sec, 37.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4713, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 262, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (281.66, 143.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1065.280 CPU sec, 35.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4746, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 261, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (285.18, 142.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1035.313 CPU sec, 34.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4955, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 270, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (287.70, 143.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1052.086 CPU sec, 35.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4833, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 270, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (287.29, 144.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1095.584 CPU sec, 36.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4757, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 268, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (287.71, 144.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1112.435 CPU sec, 37.172 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4705, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 261, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (281.49, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1062.058 CPU sec, 35.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4600, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 255, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (278.39, 140.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1104.992 CPU sec, 36.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4758, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 258, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (284.09, 144.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1150.013 CPU sec, 38.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4851, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 266, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (288.53, 145.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1192.749 CPU sec, 40.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4556, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 262, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (283.39, 142.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1205.266 CPU sec, 40.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4510, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 254, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (278.68, 139.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1116.531 CPU sec, 37.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4726, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 252, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (274.74, 139.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1090.353 CPU sec, 36.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4546, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 263, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (281.75, 141.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1121.395 CPU sec, 37.931 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4615, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 267, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (285.10, 140.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1178.162 CPU sec, 39.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4680, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 266, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (284.24, 141.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1153.226 CPU sec, 39.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4679, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 255, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (276.56, 138.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1063.505 CPU sec, 36.070 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4661, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 250, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (274.68, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1076.163 CPU sec, 36.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4592, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 260, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (281.23, 141.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1118.817 CPU sec, 37.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4611, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 260, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (281.54, 140.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1166.060 CPU sec, 38.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4588, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 259, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (282.28, 140.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1154.800 CPU sec, 38.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4547, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 251, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (273.39, 138.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1068.916 CPU sec, 35.838 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4817, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 262, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (282.19, 142.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1068.068 CPU sec, 35.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4981, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 272, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (288.60, 142.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1068.712 CPU sec, 35.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4986, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 265, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (285.65, 145.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1086.593 CPU sec, 36.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4713, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 263, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (284.95, 143.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1071.821 CPU sec, 36.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 4629, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 243, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (271.45, 138.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1022.896 CPU sec, 34.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4955, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 267, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (286.11, 142.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1038.049 CPU sec, 34.697 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4993, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 278, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (294.59, 147.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1081.379 CPU sec, 36.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 5018, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 266, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (287.14, 144.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1081.709 CPU sec, 36.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4790, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 258, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (280.92, 141.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1064.710 CPU sec, 35.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4592, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 249, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (272.95, 137.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1019.456 CPU sec, 34.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 4934, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 278, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (293.71, 146.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1068.918 CPU sec, 35.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 5001, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 271, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (288.67, 145.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1069.327 CPU sec, 35.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4782, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 271, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (287.94, 144.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1099.537 CPU sec, 37.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4646, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 257, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (280.68, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1051.300 CPU sec, 35.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4622, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 253, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (276.39, 137.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1039.243 CPU sec, 34.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4936, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 267, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (285.65, 141.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1117.819 CPU sec, 37.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4891, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 264, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (284.95, 141.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1114.307 CPU sec, 37.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4826, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 262, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (283.21, 141.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1104.042 CPU sec, 36.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4587, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 250, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (274.70, 138.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1027.097 CPU sec, 34.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 4633, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 251, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (274.40, 136.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1058.340 CPU sec, 36.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 5098, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 273, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (290.17, 143.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1124.331 CPU sec, 38.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 4837, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 273, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (291.22, 143.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1128.082 CPU sec, 37.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4738, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 265, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (285.41, 141.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1098.820 CPU sec, 37.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4504, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 246, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (271.07, 135.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1034.022 CPU sec, 34.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4743, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 256, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (279.81, 142.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 965.576 CPU sec, 32.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4986, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 271, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (287.74, 142.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 999.199 CPU sec, 33.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4913, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 267, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (286.56, 141.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1134.370 CPU sec, 37.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4617, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 253, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (278.38, 143.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1113.333 CPU sec, 37.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4343, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 242, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (270.42, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1051.685 CPU sec, 35.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 4505, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 244, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (273.59, 140.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1064.693 CPU sec, 35.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4597, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 260, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (282.64, 141.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1075.760 CPU sec, 35.943 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4741, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 271, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (288.40, 144.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1091.013 CPU sec, 36.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4908, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 269, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (286.96, 141.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1027.869 CPU sec, 34.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4786, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 265, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (282.91, 140.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1047.786 CPU sec, 35.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4618, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 251, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (275.72, 139.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1035.371 CPU sec, 34.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4734, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 262, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (281.83, 142.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1066.099 CPU sec, 35.629 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4898, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 268, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (283.74, 143.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1052.450 CPU sec, 35.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 5028, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 266, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (284.90, 142.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1008.772 CPU sec, 34.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4877, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 256, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (279.46, 140.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1040.803 CPU sec, 35.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4482, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 246, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (271.47, 137.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1038.540 CPU sec, 34.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4998, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 265, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (286.75, 144.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1058.210 CPU sec, 35.393 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4932, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 257, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (281.63, 143.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1049.472 CPU sec, 35.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4776, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 266, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (287.77, 144.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1051.847 CPU sec, 35.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4682, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 260, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (281.14, 140.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1018.556 CPU sec, 34.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4558, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 254, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (276.29, 138.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1077.878 CPU sec, 36.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4629, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 260, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (281.34, 141.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1114.228 CPU sec, 37.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4628, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 249, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (273.40, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1074.208 CPU sec, 35.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4574, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 260, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (279.84, 140.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1087.135 CPU sec, 36.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4584, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 255, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (276.27, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1049.783 CPU sec, 35.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4715, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 253, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (277.82, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1069.029 CPU sec, 35.734 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4643, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 265, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (283.64, 141.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1088.179 CPU sec, 36.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4685, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 254, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (276.45, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1027.128 CPU sec, 34.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4732, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 246, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (275.60, 139.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1075.410 CPU sec, 35.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 4607, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 246, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (270.43, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1062.765 CPU sec, 35.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4578, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 260, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (280.29, 140.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1119.987 CPU sec, 37.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4532, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 255, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (278.68, 140.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1126.972 CPU sec, 38.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4717, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 254, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (278.44, 140.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1072.654 CPU sec, 36.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4639, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 251, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (274.04, 139.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1070.884 CPU sec, 35.830 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 4577, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 244, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (269.30, 132.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1039.780 CPU sec, 34.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4890, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 262, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (286.02, 143.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1084.195 CPU sec, 37.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4864, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 266, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (284.32, 140.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1061.279 CPU sec, 35.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 5049, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 270, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (289.35, 145.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1061.278 CPU sec, 35.440 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4566, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 254, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (277.45, 140.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1059.869 CPU sec, 35.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4771, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 250, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (275.28, 139.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1018.606 CPU sec, 34.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4879, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 268, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (286.00, 144.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1095.120 CPU sec, 36.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 5012, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 265, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (285.76, 142.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1081.768 CPU sec, 36.169 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 5044, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 268, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (288.39, 144.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1073.090 CPU sec, 35.851 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4660, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 256, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (275.95, 138.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1063.215 CPU sec, 35.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4758, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 263, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (282.34, 141.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1066.477 CPU sec, 35.648 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4831, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 264, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (283.54, 142.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1073.196 CPU sec, 35.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4916, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 268, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (286.52, 143.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1076.381 CPU sec, 36.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4538, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 260, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (281.38, 140.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1074.806 CPU sec, 35.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4260, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 231, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (261.37, 130.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1088.302 CPU sec, 36.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4587, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 265, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (284.24, 142.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1139.233 CPU sec, 38.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4679, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 261, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (282.96, 142.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1115.535 CPU sec, 37.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4721, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 263, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (286.42, 144.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1105.720 CPU sec, 36.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4427, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 243, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (270.35, 138.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1065.217 CPU sec, 35.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 4317, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 243, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (266.93, 133.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1056.819 CPU sec, 35.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 4584, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 262, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (282.06, 141.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1135.606 CPU sec, 37.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4627, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 264, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (282.56, 140.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1130.467 CPU sec, 38.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4777, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 259, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (281.39, 141.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1104.119 CPU sec, 36.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4395, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 241, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (267.12, 136.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1099.333 CPU sec, 36.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4588, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 253, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (275.52, 139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1104.976 CPU sec, 37.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 4736, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 267, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (284.52, 141.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1132.192 CPU sec, 37.824 real sec [M::process] read 33222 sequences (5016522 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 4908, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 263, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (284.26, 141.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 1151.084 CPU sec, 38.859 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 2393, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 266, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (287.88, 142.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 33222 reads in 574.466 CPU sec, 19.275 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21188\tSM:UG10S2P655\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21188_HLKNYBGXX_UG10S2P655_R1.fastq.gz 5347_7332_21188_HLKNYBGXX_UG10S2P655_R2.fastq.gz [main] Real time: 4165.198 sec; CPU: 123973.643 sec Finished alignement of UG10S2P655 at Wed Apr 3 22:01:00 2019 Sorting BAM file started at Wed Apr 3 22:01:00 2019 samtools sort -@ 30 -m 4G UG10S2P655_unsorted.bam -o UG10S2P655.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 22:01:00 2019 rm UG10S2P655_unsorted.bam rm 5347_7332_21188_HLKNYBGXX_UG10S2P655_R1.fastq.gz rm 5347_7332_21188_HLKNYBGXX_UG10S2P655_R2.fastq.gz samtools index UG10S2P655.bam Finished BAM file generation of the sample - UG10S2P655 Processing fastq files for the sample - UG10S2P660 Reading 5347_7332_21189_HLKNYBGXX_UG10S2P660_R1.fastq.gz 5347_7332_21189_HLKNYBGXX_UG10S2P660_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21189\tSM:UG10S2P660\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21189_HLKNYBGXX_UG10S2P660_R1.fastq.gz 5347_7332_21189_HLKNYBGXX_UG10S2P660_R2.fastq.gz | samtools view -Sb1 - > UG10S2P660_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13167, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 102, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (132.32, 69.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.49, 127.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.626 CPU sec, 7.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13303, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (163.86, 88.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (253.50, 127.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.563 CPU sec, 5.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13508, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (159.40, 70.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (252.93, 127.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.859 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13517, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 143, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (157.00, 119.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (255.25, 130.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.466 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13749, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 148, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (156.87, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (254.60, 126.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.590 CPU sec, 6.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13618, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 108, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (112.20, 69.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (257.47, 129.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.655 CPU sec, 5.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13936, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 165, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (186.04, 121.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (255.11, 128.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.083 CPU sec, 4.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13732, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 168, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (186.21, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (254.88, 128.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.586 CPU sec, 5.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13718, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 182, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (185.21, 96.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 224, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (256.75, 130.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.926 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13762, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (141.87, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.59, 128.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.555 CPU sec, 4.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13756, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 197, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (197.17, 108.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (256.40, 129.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.578 CPU sec, 6.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13873, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 141, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (173.85, 109.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 222, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (256.42, 129.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.529 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13751, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 153, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (159.52, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (257.05, 130.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.428 CPU sec, 7.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13728, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 168, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (199.20, 128.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.44, 128.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.991 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13871, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 164, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (146.26, 76.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (255.12, 128.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.043 CPU sec, 5.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13706, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 131, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (179.48, 116.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (252.50, 127.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.865 CPU sec, 7.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13869, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 115, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (153.50, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (253.71, 128.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.522 CPU sec, 6.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13833, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 123, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 256) [M::mem_pestat] mean and std.dev: (131.30, 45.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (253.10, 126.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.931 CPU sec, 5.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13711, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 169, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (194.72, 123.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.44, 126.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.219 CPU sec, 5.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13762, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 120, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (139.22, 81.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (250.19, 126.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.574 CPU sec, 6.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13658, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 181, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (194.57, 110.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (249.76, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.479 CPU sec, 6.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13690, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 165, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (159.86, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (252.06, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.025 CPU sec, 6.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13846, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 183, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (193.33, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.39, 124.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.222 CPU sec, 5.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13749, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (183.19, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.54, 125.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.640 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13608, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 154, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (156.69, 116.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (247.66, 123.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.191 CPU sec, 5.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13626, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 128, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (164.12, 110.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.19, 124.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.288 CPU sec, 5.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13176, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 177, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (216.29, 135.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (252.16, 126.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.355 CPU sec, 5.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13506, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 172, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (150.18, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (251.71, 126.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.707 CPU sec, 7.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13555, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 187, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (192.85, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 223, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (253.19, 126.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.897 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13531, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 138, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (143.67, 69.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (252.05, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.957 CPU sec, 7.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13524, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 141, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (169.80, 103.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (252.56, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.235 CPU sec, 7.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13519, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 155, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (180.00, 123.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (253.03, 127.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.407 CPU sec, 6.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13588, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 166, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (192.28, 134.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (255.39, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.342 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13832, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 231, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (209.68, 112.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (255.84, 128.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.481 CPU sec, 5.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13897, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 134, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (162.45, 94.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (255.93, 129.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.140 CPU sec, 5.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13850, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (157.68, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (255.37, 128.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.367 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13791, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 171, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (157.47, 85.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.35, 127.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.425 CPU sec, 5.675 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13755, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (160.71, 109.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (255.75, 129.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.426 CPU sec, 5.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13980, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 166, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (178.56, 97.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (254.13, 129.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.696 CPU sec, 6.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13769, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 145, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (188.29, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (252.95, 126.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.925 CPU sec, 5.754 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13900, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (111.67, 61.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (253.24, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.972 CPU sec, 5.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13877, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 136, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (167.38, 119.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.43, 127.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.328 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13797, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 177, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (152.56, 84.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.77, 127.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.086 CPU sec, 5.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13718, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 146, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (149.72, 101.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.07, 127.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.448 CPU sec, 5.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13818, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 141, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (168.40, 100.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (251.99, 125.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.591 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13682, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 154, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (164.23, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 215, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (248.64, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.268 CPU sec, 6.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13715, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 143, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (188.94, 136.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (249.98, 126.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.921 CPU sec, 5.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13630, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 146, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (156.06, 88.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.91, 124.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.330 CPU sec, 8.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13653, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 220, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (205.00, 145.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (247.95, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.912 CPU sec, 5.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13760, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 152, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (165.35, 104.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (247.33, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.636 CPU sec, 6.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13625, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 135, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (128.17, 64.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.55, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.327 CPU sec, 5.434 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13470, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 212, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (199.50, 108.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (247.91, 123.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.118 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13387, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 163, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (175.96, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.29, 125.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.105 CPU sec, 5.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13472, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 141, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (158.73, 102.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (253.58, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.775 CPU sec, 5.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13278, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 200, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (181.50, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (251.60, 127.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.890 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13457, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 160, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (191.83, 122.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.83, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.481 CPU sec, 5.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13429, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 166, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (157.44, 87.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (253.74, 127.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.851 CPU sec, 5.701 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13625, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 140, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (129.11, 60.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (253.40, 127.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.214 CPU sec, 6.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13585, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 148, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (150.31, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (252.24, 126.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.258 CPU sec, 5.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13643, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 175, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (187.83, 113.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (253.92, 128.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.109 CPU sec, 5.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13740, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 167, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (160.69, 83.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (253.78, 127.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.285 CPU sec, 5.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13597, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (128.00, 53.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (254.11, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.464 CPU sec, 5.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13685, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 151, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (137.41, 58.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.28, 128.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.246 CPU sec, 6.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13752, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 170, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (193.31, 110.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (255.42, 128.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.663 CPU sec, 6.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13647, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (141.05, 57.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (253.93, 127.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.774 CPU sec, 5.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13756, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 282, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (239.85, 132.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (250.97, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.898 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13610, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 132, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (143.19, 70.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (251.49, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.134 CPU sec, 5.066 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13655, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 169, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (179.56, 94.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (251.35, 127.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.697 CPU sec, 4.805 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13596, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 166, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (173.53, 101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.51, 125.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.185 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13657, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 152, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (218.12, 113.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (249.89, 125.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.690 CPU sec, 4.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13821, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 220, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (223.16, 134.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (252.29, 126.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.386 CPU sec, 4.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13703, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 141, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (157.20, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (248.15, 124.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.596 CPU sec, 4.617 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13584, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 174, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (184.67, 109.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (248.61, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.532 CPU sec, 4.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13560, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 156, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (194.60, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (247.92, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.380 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13444, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (142.10, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (245.74, 122.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.147 CPU sec, 4.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13578, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 160, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (169.54, 101.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (248.38, 123.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.816 CPU sec, 4.689 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13625, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 149, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (152.04, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.75, 123.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.916 CPU sec, 4.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13601, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 172, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (173.00, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.59, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.491 CPU sec, 4.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13491, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 160, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (193.78, 123.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (245.11, 123.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.543 CPU sec, 4.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13506, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 164, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (171.33, 81.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.75, 122.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.928 CPU sec, 6.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13631, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 164, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (185.78, 116.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.25, 123.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.143 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13621, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 116, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (138.42, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (250.19, 125.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.184 CPU sec, 6.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13557, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 254, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (248.27, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.60, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.030 CPU sec, 5.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13489, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 142, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (174.72, 106.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (251.77, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.594 CPU sec, 6.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13784, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 183, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (171.59, 112.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (253.54, 127.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.073 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13720, 7, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 140, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (165.62, 83.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (251.91, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.144 CPU sec, 5.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13866, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 142, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (172.54, 127.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.46, 127.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.207 CPU sec, 6.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13726, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 141, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (181.08, 132.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (253.51, 127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.402 CPU sec, 6.565 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13862, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 163, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (143.06, 58.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (253.04, 127.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.395 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13784, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 258, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (204.00, 152.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (252.31, 126.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.087 CPU sec, 4.872 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13832, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 172, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (199.86, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (251.55, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.081 CPU sec, 4.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13760, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 107, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (175.55, 155.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.88, 126.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.684 CPU sec, 4.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13821, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 177, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 319) [M::mem_pestat] mean and std.dev: (166.64, 56.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (252.15, 126.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.174 CPU sec, 5.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13776, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 182, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (199.90, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (250.80, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.588 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13659, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 165, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (178.00, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.43, 125.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.162 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13687, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 122, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (149.05, 105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (247.92, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.143 CPU sec, 5.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13734, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 151, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (172.35, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (247.84, 122.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.988 CPU sec, 6.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13678, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 130, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (154.63, 88.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (246.93, 123.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.116 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13561, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (151.44, 100.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.63, 125.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.100 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13651, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 144, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (171.21, 114.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.52, 121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.631 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13846, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 180, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (210.24, 121.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (245.53, 122.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.334 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13642, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 189, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (184.96, 115.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (247.86, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.272 CPU sec, 5.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13667, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 141, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (164.56, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (247.19, 123.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.202 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13556, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 142, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (153.59, 114.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (245.46, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.534 CPU sec, 5.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13237, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 123, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (115.93, 56.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (243.29, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.750 CPU sec, 6.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13261, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 139, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (180.55, 119.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (243.70, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.508 CPU sec, 5.548 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13343, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 174, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (158.83, 88.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (245.37, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.658 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13571, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 178, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (166.37, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.29, 124.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.223 CPU sec, 5.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13569, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 173, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (194.54, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.57, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.235 CPU sec, 6.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13644, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 141, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (185.33, 128.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (249.94, 127.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.023 CPU sec, 5.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13737, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 152, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (159.38, 99.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (251.38, 127.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.097 CPU sec, 6.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13742, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 151, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (172.10, 89.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.35, 125.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.831 CPU sec, 5.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13603, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 278, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (254.36, 114.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.59, 126.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.991 CPU sec, 5.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13739, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 164, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (177.30, 109.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (254.09, 129.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.394 CPU sec, 5.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13728, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 194, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (220.67, 134.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (250.00, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.217 CPU sec, 5.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13647, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (127.13, 49.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (251.90, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.369 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13748, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 159, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (158.96, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.17, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.428 CPU sec, 4.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13777, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (138.47, 68.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.13, 125.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.336 CPU sec, 4.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13611, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 143, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (177.35, 105.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (250.09, 125.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.919 CPU sec, 4.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13746, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 188, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (186.88, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (249.28, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.987 CPU sec, 4.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13798, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 132, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (160.19, 110.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (249.32, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.811 CPU sec, 4.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13894, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (166.12, 71.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.08, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.679 CPU sec, 4.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13624, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 155, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (171.04, 111.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.64, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.259 CPU sec, 4.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13617, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 126, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (152.48, 99.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 209, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (243.13, 121.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.559 CPU sec, 3.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13627, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 188, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (189.71, 106.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (247.43, 122.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.289 CPU sec, 4.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13596, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 130, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (187.64, 146.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (246.65, 122.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.024 CPU sec, 4.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13714, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 179, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (168.80, 81.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (246.90, 124.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.765 CPU sec, 4.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13635, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 110, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (132.15, 79.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (244.07, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.798 CPU sec, 4.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13673, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 118, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (126.55, 65.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (245.47, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.036 CPU sec, 4.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13519, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 135, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (122.90, 57.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.09, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.557 CPU sec, 4.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13422, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 140, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (152.38, 94.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (246.11, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.143 CPU sec, 5.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13214, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 186, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (192.00, 129.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (241.33, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.364 CPU sec, 5.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13495, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 139, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (145.35, 48.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (247.67, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.239 CPU sec, 5.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13521, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 120, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (155.75, 106.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (247.05, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.941 CPU sec, 5.202 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13575, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 132, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (149.21, 76.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.08, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.335 CPU sec, 5.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13573, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 130, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (122.57, 61.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (247.93, 124.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.510 CPU sec, 4.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13675, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 197, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (184.45, 78.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (247.26, 125.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.924 CPU sec, 4.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13619, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 166, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (162.36, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 221, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (251.75, 126.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.022 CPU sec, 4.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13696, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 169, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (175.11, 92.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (249.11, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.043 CPU sec, 4.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13676, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (106.93, 57.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (248.80, 124.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.129 CPU sec, 4.142 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13756, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 189, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (182.67, 100.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.72, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.116 CPU sec, 5.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13666, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 160, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (149.53, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (250.54, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.071 CPU sec, 4.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13793, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 189, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (204.09, 121.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (250.57, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.655 CPU sec, 5.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13593, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (163.83, 104.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 215, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.26, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.765 CPU sec, 5.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13651, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (132.35, 58.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 215, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (248.99, 124.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.872 CPU sec, 4.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13499, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 165, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (174.46, 126.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (249.35, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.780 CPU sec, 4.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13437, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 167, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (221.44, 170.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (246.96, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.422 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13696, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 159, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (182.26, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.42, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.638 CPU sec, 5.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13628, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 201, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (224.64, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (245.45, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.536 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13612, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (175.88, 108.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (245.46, 121.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.321 CPU sec, 4.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13530, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 145, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (172.58, 111.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (242.66, 120.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.910 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13573, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 132, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (137.50, 87.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.59, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.700 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13405, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 137, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (158.40, 90.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (242.76, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.622 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13512, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (153.86, 101.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.70, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.320 CPU sec, 4.522 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13407, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (108.42, 61.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (244.32, 120.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.317 CPU sec, 4.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13428, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 145, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (154.55, 84.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (246.08, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.973 CPU sec, 4.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13722, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 169, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (197.22, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (255.65, 127.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.768 CPU sec, 4.164 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13501, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 159, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (170.00, 101.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (257.90, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.741 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13576, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 163, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (154.29, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (255.21, 128.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.363 CPU sec, 4.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13801, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 142, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (150.27, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (254.85, 128.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.355 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13783, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 103, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (166.47, 109.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (254.78, 128.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.178 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13545, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 193, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (187.95, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (256.18, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.660 CPU sec, 4.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13769, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (193.23, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (255.37, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.745 CPU sec, 4.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13727, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 190, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (219.08, 118.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (254.16, 127.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.722 CPU sec, 4.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13704, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 143, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (146.53, 84.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (252.63, 126.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.792 CPU sec, 5.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13735, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (133.95, 61.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.27, 127.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.976 CPU sec, 4.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13574, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 273, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (232.79, 143.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 222, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (255.50, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.308 CPU sec, 4.350 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13712, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 178, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (179.24, 100.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (253.30, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.364 CPU sec, 4.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13712, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (198.77, 105.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.33, 126.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.919 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13669, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 157, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (193.90, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.54, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.255 CPU sec, 4.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13650, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 157, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (152.22, 63.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.30, 126.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.478 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13661, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 197, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (190.00, 85.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (252.15, 128.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.805 CPU sec, 4.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13597, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 220, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (234.29, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (250.71, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.110 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13674, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 108, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (95.50, 35.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (252.40, 127.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.903 CPU sec, 5.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13619, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 134, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (156.14, 97.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (248.15, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.005 CPU sec, 7.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13660, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (153.18, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (247.29, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.451 CPU sec, 5.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13610, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (137.47, 99.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 213, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (245.27, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.821 CPU sec, 5.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13618, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 154, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (161.92, 59.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (247.03, 123.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.638 CPU sec, 6.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13463, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 258, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (226.35, 129.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (243.72, 121.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.889 CPU sec, 6.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13331, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 169, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (182.61, 117.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 213, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (243.89, 120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.705 CPU sec, 5.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12956, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 155, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (178.05, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (241.01, 119.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.826 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12857, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 220, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (211.20, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (241.46, 120.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.710 CPU sec, 6.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13432, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 176, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (206.42, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (253.44, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.325 CPU sec, 6.115 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13840, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 187, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (215.13, 133.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (258.16, 130.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.896 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13737, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 172, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (182.60, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 227, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (259.05, 129.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.977 CPU sec, 4.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13621, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 161, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (177.85, 101.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 221, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (254.49, 128.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.291 CPU sec, 4.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13834, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 92, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (145.91, 96.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (257.26, 128.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.633 CPU sec, 5.244 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13699, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 106, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (143.44, 100.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (256.55, 128.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.889 CPU sec, 4.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13821, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 192, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (190.11, 110.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (256.52, 128.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.106 CPU sec, 5.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13816, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 207, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (218.76, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (258.28, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.249 CPU sec, 5.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 13693, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 163, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (207.95, 134.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 227, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (259.60, 130.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.659 CPU sec, 5.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13732, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 137, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (178.30, 113.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (258.52, 130.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.237 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13685, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 139, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (167.35, 95.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (255.52, 128.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.734 CPU sec, 6.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13799, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 145, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (194.19, 132.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (258.51, 130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.561 CPU sec, 5.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13799, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 163, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (164.82, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 223, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (257.00, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.278 CPU sec, 5.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13752, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 220, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (187.37, 121.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (255.16, 128.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.657 CPU sec, 5.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13775, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 103, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (119.00, 76.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (256.36, 129.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.152 CPU sec, 6.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13690, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 206, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (192.62, 102.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (255.10, 127.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.999 CPU sec, 4.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13717, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 150, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (156.08, 85.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (253.39, 128.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.616 CPU sec, 7.469 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13895, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 175, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (156.89, 111.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (252.79, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.931 CPU sec, 5.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13670, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 230, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (210.00, 107.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (250.79, 125.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.967 CPU sec, 5.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13560, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 188, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (174.05, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (246.41, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.870 CPU sec, 5.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13429, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (171.91, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 210, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (244.42, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.884 CPU sec, 6.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13403, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 182, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (200.88, 119.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (243.72, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.527 CPU sec, 5.785 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13354, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 124, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (129.75, 79.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.09, 121.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.086 CPU sec, 6.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13007, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 261, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (255.09, 106.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (239.65, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.670 CPU sec, 5.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13001, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 171, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (181.06, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (240.33, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.637 CPU sec, 6.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12808, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 183, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (182.24, 85.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (242.07, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.731 CPU sec, 7.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13247, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 130, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (150.56, 86.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.88, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.290 CPU sec, 6.404 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 13668, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 182, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (188.14, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 226, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (260.53, 132.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.457 CPU sec, 5.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13734, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (127.23, 60.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (255.17, 128.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.145 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13498, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 172, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (196.88, 98.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (257.76, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.662 CPU sec, 4.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13590, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (153.20, 116.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 227, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (258.61, 129.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.481 CPU sec, 5.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13842, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 117, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (156.86, 115.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (256.19, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.731 CPU sec, 6.034 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13611, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 173, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (177.04, 111.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (256.65, 130.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.580 CPU sec, 6.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13710, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 142, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (164.00, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (256.57, 129.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.985 CPU sec, 6.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13716, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (148.90, 92.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (257.55, 131.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.717 CPU sec, 5.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13797, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 121, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (128.53, 55.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (255.94, 129.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.506 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13670, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (172.32, 88.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (257.19, 128.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.760 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13812, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 213, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (180.41, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (253.44, 127.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.029 CPU sec, 5.051 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13598, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 147, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (156.70, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (255.68, 128.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.942 CPU sec, 5.845 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13595, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 173, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (190.33, 105.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (252.61, 127.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.755 CPU sec, 5.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13767, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 184, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (201.80, 128.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (254.90, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.068 CPU sec, 5.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13673, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 94, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (168.81, 137.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.55, 127.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.307 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13775, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 185, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (184.38, 103.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (250.96, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.319 CPU sec, 5.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13562, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 173, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (178.36, 109.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.76, 126.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.040 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13563, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 132, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (184.12, 133.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 219, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (250.49, 123.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.379 CPU sec, 6.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13523, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (167.20, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (246.23, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.225 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13464, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 140, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (178.32, 121.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (246.09, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.241 CPU sec, 5.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13373, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 164, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (174.70, 115.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (241.00, 120.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.103 CPU sec, 5.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13339, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 114, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (146.71, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (241.29, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.578 CPU sec, 5.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 13119, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 144, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (176.36, 91.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (240.38, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.244 CPU sec, 5.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13027, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 151, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (164.57, 94.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (242.17, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.306 CPU sec, 5.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12812, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 151, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (209.55, 140.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (242.35, 119.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.531 CPU sec, 6.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13190, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (117.46, 78.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.87, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.557 CPU sec, 6.880 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13680, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 191, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (184.25, 115.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (251.80, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.680 CPU sec, 6.000 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13725, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 137, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (127.05, 52.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.21, 124.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.420 CPU sec, 4.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13565, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 82, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (135.84, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (249.18, 124.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.091 CPU sec, 5.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13616, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 172, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (184.20, 119.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (252.66, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.450 CPU sec, 4.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13740, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 136, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (167.37, 108.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (252.66, 125.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.667 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13633, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 138, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (145.28, 60.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.72, 126.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.069 CPU sec, 6.074 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13634, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 269, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (205.62, 105.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (250.29, 125.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.522 CPU sec, 4.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13635, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 186, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (207.00, 104.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (252.49, 127.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.869 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13882, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 114, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (122.58, 55.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (252.09, 126.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.116 CPU sec, 4.640 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13593, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (206.58, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.84, 126.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.552 CPU sec, 5.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13694, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 136, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (169.75, 107.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.08, 127.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.601 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13574, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (151.81, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (252.94, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.049 CPU sec, 5.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13873, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (197.41, 124.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (251.51, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.261 CPU sec, 6.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13645, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 96, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (141.82, 130.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (249.91, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.459 CPU sec, 4.445 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13583, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 146, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (159.07, 94.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (249.19, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.292 CPU sec, 4.802 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13675, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 156, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (174.46, 122.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.63, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.413 CPU sec, 5.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13874, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 128, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (151.65, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (249.21, 124.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.105 CPU sec, 5.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13514, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 193, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (195.06, 102.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.90, 123.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.404 CPU sec, 5.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13483, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 136, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (156.39, 84.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (243.17, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.760 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13509, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (146.87, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (242.86, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.298 CPU sec, 6.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13412, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (196.37, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (241.75, 120.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.926 CPU sec, 5.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13306, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 153, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (148.61, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.68, 118.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.940 CPU sec, 6.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13146, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 183, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (193.35, 100.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (239.36, 117.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.817 CPU sec, 6.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13049, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 151, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (150.33, 63.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (237.16, 117.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.228 CPU sec, 8.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12864, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 202, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (195.79, 131.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.28, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.198 CPU sec, 6.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13499, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 162, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (192.70, 130.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (248.87, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.193 CPU sec, 5.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13648, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 167, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (161.72, 103.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (255.18, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.147 CPU sec, 5.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13628, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 159, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (150.42, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (253.95, 127.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.356 CPU sec, 5.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13581, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 149, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (174.16, 97.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (253.13, 126.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.210 CPU sec, 5.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13752, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 156, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (167.80, 95.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (252.12, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.446 CPU sec, 6.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13713, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 146, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (115.00, 76.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 220, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (254.35, 127.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.998 CPU sec, 5.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13683, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (136.43, 104.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.71, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.367 CPU sec, 5.706 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13711, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 159, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (178.37, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (256.13, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.608 CPU sec, 6.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13651, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 147, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (166.81, 80.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (253.24, 127.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.686 CPU sec, 6.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13760, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 173, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (190.95, 98.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (254.90, 127.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.275 CPU sec, 6.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13744, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 192, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (171.74, 88.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (255.18, 128.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.124 CPU sec, 5.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13792, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 172, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (174.70, 99.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (254.18, 128.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.948 CPU sec, 6.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13878, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (190.55, 119.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (251.20, 126.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.119 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13702, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 179, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (176.75, 106.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (253.63, 128.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.068 CPU sec, 5.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13746, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 171, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (206.85, 123.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.14, 127.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.953 CPU sec, 5.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13647, 10, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 138, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (189.29, 138.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (250.00, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 219, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5381) [M::mem_pestat] mean and std.dev: (336.50, 573.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7154) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 153.021 CPU sec, 5.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13611, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 185, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (196.11, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.06, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.267 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13558, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 172, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (186.21, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (248.87, 124.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.802 CPU sec, 6.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13510, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 150, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (173.04, 83.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (243.09, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.740 CPU sec, 5.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13508, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 147, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (190.80, 98.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (246.17, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.587 CPU sec, 5.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13583, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 210, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (236.00, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (241.11, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.520 CPU sec, 5.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13411, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 205, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (212.53, 114.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.89, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.024 CPU sec, 6.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13274, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (154.12, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 208, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (240.33, 118.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.205 CPU sec, 5.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13177, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 162, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (189.95, 112.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (238.26, 117.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.425 CPU sec, 5.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12721, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 214, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (189.90, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (234.33, 114.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.688 CPU sec, 6.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12828, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 181, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (192.18, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (238.78, 116.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.945 CPU sec, 6.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13553, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 150, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (155.76, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (252.22, 126.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.675 CPU sec, 5.870 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13684, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (209.94, 129.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 225, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (256.51, 128.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.888 CPU sec, 5.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13836, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 169, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (189.26, 100.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (254.38, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.258 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13686, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 166, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (207.22, 183.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 223, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (254.71, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.533 CPU sec, 5.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13617, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 142, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (163.78, 99.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (255.21, 129.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.628 CPU sec, 5.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13795, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 218, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (198.00, 99.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (252.78, 126.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.780 CPU sec, 6.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13604, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 169, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (190.60, 116.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (252.94, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.559 CPU sec, 5.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13570, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 106, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (138.52, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.65, 126.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.827 CPU sec, 5.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13734, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 151, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (171.35, 92.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 221, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (253.61, 126.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.312 CPU sec, 5.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13693, 8, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (148.61, 98.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (255.28, 129.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.906 CPU sec, 6.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13840, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 205, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (214.59, 147.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.32, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.738 CPU sec, 5.106 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13647, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 149, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (144.54, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (252.64, 127.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.863 CPU sec, 5.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13667, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 213, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (226.86, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 220, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (252.80, 126.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.497 CPU sec, 6.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13760, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 168, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (155.29, 85.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.84, 126.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.529 CPU sec, 5.316 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13729, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 155, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (210.33, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.24, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.021 CPU sec, 4.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13728, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 163, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (173.80, 75.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.20, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.795 CPU sec, 5.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13674, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 122, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (142.52, 60.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (248.83, 124.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.875 CPU sec, 6.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13650, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 119, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (160.96, 108.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.77, 123.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.598 CPU sec, 5.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13501, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 164, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (173.43, 98.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.41, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.371 CPU sec, 5.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13607, 6, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 123, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (146.50, 109.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (244.70, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.711 CPU sec, 5.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13288, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (158.19, 90.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (243.28, 121.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.044 CPU sec, 5.591 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13420, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 158, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (177.71, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (242.65, 121.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.681 CPU sec, 6.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13172, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 139, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (144.16, 68.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (239.17, 117.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.011 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13183, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 136, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (158.81, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (239.73, 117.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.594 CPU sec, 6.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12684, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 161, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (170.96, 83.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 201, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (233.22, 114.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.982 CPU sec, 5.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13082, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 152, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (189.15, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 207, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (239.91, 118.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.267 CPU sec, 6.680 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13674, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 149, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (169.67, 110.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (247.95, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.714 CPU sec, 6.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13668, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (172.33, 115.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (249.06, 124.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.679 CPU sec, 6.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13772, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 125, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (148.65, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.12, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.225 CPU sec, 5.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13531, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 157, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (172.57, 90.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (245.88, 122.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.256 CPU sec, 6.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 13554, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 172, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (193.68, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (245.11, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.381 CPU sec, 5.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13575, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (160.30, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (245.17, 121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.002 CPU sec, 6.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13518, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 148, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (185.36, 114.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.74, 122.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.568 CPU sec, 5.263 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13660, 7, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 142, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (163.64, 91.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.99, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.586 CPU sec, 5.784 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13776, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 177, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (173.71, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (254.86, 129.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.635 CPU sec, 6.201 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13732, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 143, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (166.76, 107.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (252.86, 127.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.109 CPU sec, 4.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13780, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 169, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (160.05, 100.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (251.62, 127.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.064 CPU sec, 5.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13918, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 165, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (160.89, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (253.42, 128.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.225 CPU sec, 5.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13727, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 166, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (167.00, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.89, 127.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.271 CPU sec, 6.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13578, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 143, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (141.27, 83.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.18, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.620 CPU sec, 6.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13827, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 149, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (182.17, 101.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (253.19, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.801 CPU sec, 5.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13630, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 154, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (165.54, 93.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.09, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.945 CPU sec, 6.595 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13372, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 202, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (209.00, 140.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (250.36, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.022 CPU sec, 6.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13241, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (213.50, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (249.96, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.088 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13477, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 120, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (135.11, 78.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (250.06, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.908 CPU sec, 6.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13518, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 152, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (184.21, 132.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.04, 126.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.384 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13427, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 176, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (216.43, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (251.12, 126.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.915 CPU sec, 5.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13518, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 204, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (183.00, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.19, 126.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.245 CPU sec, 6.165 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13725, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 141, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (187.04, 117.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (250.45, 125.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.274 CPU sec, 5.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13723, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 140, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (176.80, 115.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (252.83, 127.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.536 CPU sec, 5.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13700, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 130, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (133.93, 74.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.64, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.538 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13679, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (129.71, 53.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (249.26, 124.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.838 CPU sec, 5.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13670, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 170, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (193.39, 99.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.09, 122.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.123 CPU sec, 5.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13658, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 104, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (137.26, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.67, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.715 CPU sec, 5.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13710, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 225, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (225.92, 153.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (246.36, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.730 CPU sec, 7.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13665, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 167, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (166.57, 90.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.11, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.188 CPU sec, 6.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13536, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 137, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (182.80, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (246.18, 122.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.228 CPU sec, 6.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13491, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 159, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (176.57, 90.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (246.27, 122.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.263 CPU sec, 6.656 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13613, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 160, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (155.38, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (244.75, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.920 CPU sec, 6.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13661, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 203, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (191.82, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (253.82, 128.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.678 CPU sec, 6.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13886, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 215, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (216.90, 143.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (251.68, 126.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.825 CPU sec, 5.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13866, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 127, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (159.12, 101.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (252.08, 127.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.879 CPU sec, 6.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13698, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 128, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (146.91, 93.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.45, 126.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.045 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13803, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 169, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (167.23, 98.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (254.11, 128.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.499 CPU sec, 4.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13695, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 163, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (172.00, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (252.18, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.790 CPU sec, 5.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13708, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (157.00, 51.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (250.96, 126.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.906 CPU sec, 5.780 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13729, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 157, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (158.90, 95.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (249.63, 125.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.934 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13516, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 177, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (180.69, 112.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (249.29, 125.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.066 CPU sec, 5.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13211, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 156, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (157.84, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.38, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.227 CPU sec, 6.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13380, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 170, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (198.47, 106.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (248.65, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.660 CPU sec, 5.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13391, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 117, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (128.69, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (249.78, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.354 CPU sec, 5.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13510, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 140, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (164.31, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (250.36, 126.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.513 CPU sec, 5.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13719, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 156, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (164.94, 117.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (251.11, 126.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.818 CPU sec, 5.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13837, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 146, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (152.00, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (251.15, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.979 CPU sec, 6.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13746, 10, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 138, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (144.15, 70.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (251.70, 126.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 1599, 2404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7112) [M::mem_pestat] mean and std.dev: (1805.00, 2004.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9821) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 159.324 CPU sec, 7.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13662, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 193, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (179.22, 94.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.98, 126.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.831 CPU sec, 5.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13699, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 166, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (166.68, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (250.38, 126.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.676 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13524, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 174, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (179.65, 89.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (246.65, 122.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.300 CPU sec, 6.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13551, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 157, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (136.43, 70.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (243.85, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.744 CPU sec, 6.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13644, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 179, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (206.96, 135.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (245.11, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.480 CPU sec, 5.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13484, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 203, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (199.05, 95.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (243.63, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.637 CPU sec, 6.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13594, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 186, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (176.38, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (243.25, 119.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.085 CPU sec, 6.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13588, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 184, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (174.59, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (242.08, 119.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.740 CPU sec, 5.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13350, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 142, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (169.23, 109.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (243.58, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.032 CPU sec, 5.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13749, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 178, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (186.82, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (253.69, 126.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.174 CPU sec, 5.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13853, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 102, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (125.67, 65.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (255.85, 129.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.022 CPU sec, 5.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13826, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 135, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (144.43, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (254.00, 129.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.494 CPU sec, 6.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13825, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 139, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (136.11, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (252.92, 127.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.075 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13669, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 121, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (147.83, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (252.96, 127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.557 CPU sec, 5.462 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13634, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 178, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (173.53, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (251.39, 126.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.302 CPU sec, 4.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13673, 3, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (149.24, 91.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.49, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.346 CPU sec, 5.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13698, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (212.29, 177.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (248.68, 124.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.980 CPU sec, 5.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13602, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 143, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (153.19, 76.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (248.08, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.948 CPU sec, 6.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13168, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 158, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (182.37, 113.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.84, 123.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.981 CPU sec, 6.209 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13388, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 123, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (146.30, 93.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (250.95, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.104 CPU sec, 7.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13617, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 140, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (148.83, 84.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (250.99, 125.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.460 CPU sec, 5.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13476, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 148, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (168.84, 90.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 219, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (253.15, 128.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.850 CPU sec, 5.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13600, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 163, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (161.42, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.42, 126.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.342 CPU sec, 7.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13622, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 116, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (148.27, 87.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.44, 126.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.041 CPU sec, 5.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13724, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 195, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (181.12, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (253.36, 127.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.489 CPU sec, 7.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13761, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (184.55, 123.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (252.67, 126.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.490 CPU sec, 5.282 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13569, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 147, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (201.06, 130.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.16, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.653 CPU sec, 6.569 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13224, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 129, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (148.96, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (240.94, 119.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.318 CPU sec, 6.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13563, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 134, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (155.71, 109.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (245.70, 121.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.047 CPU sec, 5.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13550, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 149, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (167.95, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (245.23, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.981 CPU sec, 6.844 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 13623, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 118, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (139.03, 94.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (242.92, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.409 CPU sec, 5.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13469, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 156, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (205.10, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.57, 121.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.603 CPU sec, 5.761 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13510, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (151.10, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (243.77, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.486 CPU sec, 5.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13409, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 170, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (222.19, 134.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (245.44, 121.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.637 CPU sec, 5.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13664, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 229, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (215.15, 107.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (249.87, 125.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.543 CPU sec, 5.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13726, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 190, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (199.68, 122.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.45, 126.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.764 CPU sec, 6.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13513, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 109, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (157.68, 128.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (249.77, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.844 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13658, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 269, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (245.22, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (249.22, 125.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.832 CPU sec, 5.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13624, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 196, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (190.80, 104.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (251.33, 126.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.104 CPU sec, 5.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13666, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 131, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (131.71, 65.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (249.30, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.277 CPU sec, 5.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13585, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 146, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (190.50, 144.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.58, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.476 CPU sec, 6.073 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13574, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 171, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (179.55, 134.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.33, 124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.098 CPU sec, 5.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13608, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 186, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (189.06, 114.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (246.99, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.023 CPU sec, 5.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13061, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 118, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (139.94, 77.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.95, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.410 CPU sec, 5.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13175, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 118, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (138.25, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.92, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.905 CPU sec, 6.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13386, 10, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 128, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (142.15, 86.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (242.98, 121.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 3331, 5265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15745) [M::mem_pestat] mean and std.dev: (3202.40, 3317.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20985) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 152.650 CPU sec, 6.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13353, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 154, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (174.07, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (245.05, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.719 CPU sec, 6.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13472, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 195, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (184.00, 106.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (244.35, 122.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.447 CPU sec, 6.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13647, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 166, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (176.50, 97.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (247.65, 125.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.891 CPU sec, 5.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13498, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 112, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (125.70, 67.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.77, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.589 CPU sec, 5.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13603, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 103, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (126.70, 55.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (247.41, 124.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.796 CPU sec, 6.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13716, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 140, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (189.11, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.58, 123.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.644 CPU sec, 5.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13776, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 174, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (170.24, 90.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (245.13, 121.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.151 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13607, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 181, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (173.57, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (246.23, 122.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.520 CPU sec, 6.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13514, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 142, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (159.62, 94.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (244.37, 120.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.170 CPU sec, 7.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13538, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (129.29, 73.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (244.15, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.949 CPU sec, 6.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13593, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 153, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (158.00, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (243.64, 121.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.102 CPU sec, 5.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13556, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 231, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (232.95, 124.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.13, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.176 CPU sec, 5.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13611, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 246, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (220.32, 133.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (244.17, 121.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.605 CPU sec, 6.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13679, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 150, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (154.71, 80.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.04, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.201 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13641, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 88, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (99.77, 56.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (251.31, 126.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.700 CPU sec, 6.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13757, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 88, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (141.91, 118.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (248.08, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.447 CPU sec, 5.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13695, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 130, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (154.45, 92.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (247.90, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.646 CPU sec, 5.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13597, 2, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 150, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (156.55, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (249.87, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.867 CPU sec, 6.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13573, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 108, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (112.00, 46.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (249.14, 124.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.694 CPU sec, 5.488 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13651, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (130.00, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (247.84, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.398 CPU sec, 5.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13765, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 107, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (139.75, 84.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (247.84, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.620 CPU sec, 5.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13715, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 131, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (168.00, 103.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (246.33, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.044 CPU sec, 5.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13161, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 202, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (184.79, 98.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (243.68, 119.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.147 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13229, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 122, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (139.39, 83.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (240.53, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.181 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13403, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 162, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (195.33, 182.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (244.51, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.983 CPU sec, 5.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13444, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 193, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (171.72, 96.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (243.16, 120.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.732 CPU sec, 5.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13514, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 129, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (168.84, 117.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (243.41, 122.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.139 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13709, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 145, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (155.43, 105.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (247.91, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.618 CPU sec, 6.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13435, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (172.00, 99.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (246.48, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.380 CPU sec, 6.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13649, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 166, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (173.24, 81.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (249.43, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.587 CPU sec, 5.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13883, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 108, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (162.04, 101.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 213, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (245.76, 123.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.402 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13521, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 136, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (169.67, 117.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (244.70, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.836 CPU sec, 5.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13658, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 183, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (168.12, 90.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (243.50, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.111 CPU sec, 6.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13704, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 157, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (160.71, 87.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (243.98, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.910 CPU sec, 5.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13560, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 131, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (152.45, 92.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (241.29, 118.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.820 CPU sec, 4.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13624, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 97, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (103.86, 71.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.61, 118.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.364 CPU sec, 5.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13510, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 155, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (141.38, 82.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (241.04, 119.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.262 CPU sec, 5.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13485, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 163, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (189.83, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.01, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.063 CPU sec, 5.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13591, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (205.19, 131.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (246.33, 122.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.711 CPU sec, 5.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13692, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 214, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (211.44, 110.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (248.50, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.565 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13604, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 151, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (167.36, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (251.26, 127.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.462 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13542, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 194, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (226.16, 156.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (247.03, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.466 CPU sec, 5.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13578, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 131, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (160.33, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (249.05, 124.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.312 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13710, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 157, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (157.75, 79.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (250.48, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.994 CPU sec, 6.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13688, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 141, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (172.42, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (245.36, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.228 CPU sec, 6.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13605, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 121, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (109.90, 57.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (243.91, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.626 CPU sec, 5.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13582, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 164, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (167.50, 114.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (245.54, 121.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.461 CPU sec, 6.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13353, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 169, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (142.75, 34.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (244.15, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.054 CPU sec, 7.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13451, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 151, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (184.83, 103.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (245.60, 120.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.394 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13382, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 135, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (167.08, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.41, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.236 CPU sec, 5.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13512, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 163, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (151.86, 51.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (245.40, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.621 CPU sec, 4.980 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13536, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 174, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (182.43, 93.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (246.23, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.732 CPU sec, 6.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13454, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 133, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (136.82, 58.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (247.77, 123.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.500 CPU sec, 6.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13639, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 152, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (171.36, 102.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (250.05, 125.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.720 CPU sec, 6.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13620, 5, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 188, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (202.67, 113.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (249.20, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.618 CPU sec, 5.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13747, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 154, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (167.68, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (248.35, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.127 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13535, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 159, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (184.53, 111.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (246.62, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.917 CPU sec, 5.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13498, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 154, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (151.93, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (242.45, 121.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.027 CPU sec, 6.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13316, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 157, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (190.20, 126.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (242.00, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.632 CPU sec, 5.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13130, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 161, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (168.74, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (240.36, 118.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.295 CPU sec, 5.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13393, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 135, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (161.86, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.86, 117.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.051 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13170, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 172, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (184.00, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (239.48, 118.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.746 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12938, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (146.96, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (240.39, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.213 CPU sec, 5.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12902, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 130, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (157.71, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 212, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (243.72, 119.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.947 CPU sec, 6.208 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13642, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 155, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (212.19, 147.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.90, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.031 CPU sec, 6.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13730, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 185, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (169.96, 102.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (253.06, 128.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.325 CPU sec, 6.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13651, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 194, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (198.00, 88.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (250.38, 125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.252 CPU sec, 6.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13621, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 94, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (92.25, 26.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (250.04, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.105 CPU sec, 5.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13734, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 136, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (154.32, 93.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (249.22, 126.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.320 CPU sec, 5.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13731, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 123, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (138.18, 84.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (249.01, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.598 CPU sec, 4.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13862, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (173.23, 115.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.88, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.240 CPU sec, 7.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13396, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 174, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (153.73, 90.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (248.17, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.185 CPU sec, 5.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13658, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 143, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (138.21, 81.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (249.32, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.738 CPU sec, 5.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13656, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 156, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (162.83, 99.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 218, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (252.07, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.472 CPU sec, 5.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13738, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 118, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (176.22, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (253.25, 127.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.955 CPU sec, 5.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13583, 8, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (169.00, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (253.09, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.676 CPU sec, 5.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13758, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 146, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (171.66, 121.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.09, 128.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.674 CPU sec, 4.952 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13896, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 162, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (200.14, 120.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 219, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.89, 126.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.977 CPU sec, 5.607 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13670, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 192, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (186.42, 88.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.80, 126.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.323 CPU sec, 5.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13741, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 137, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (159.25, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (254.29, 128.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.272 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13861, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 148, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (141.70, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (253.65, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.965 CPU sec, 6.712 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13761, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 188, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (208.18, 134.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (249.49, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.448 CPU sec, 6.543 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13511, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 201, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (187.73, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (246.02, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.227 CPU sec, 6.189 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13567, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 125, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (175.12, 107.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (243.27, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.348 CPU sec, 5.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13491, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 164, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (166.85, 96.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.77, 118.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.252 CPU sec, 5.554 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13305, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 160, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (187.19, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (241.08, 118.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.922 CPU sec, 5.914 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12840, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 130, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (138.82, 77.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (241.25, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.621 CPU sec, 7.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12953, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 106, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (112.96, 63.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (238.92, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.822 CPU sec, 6.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12932, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 212, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (227.00, 132.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (247.11, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.709 CPU sec, 6.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13545, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (176.75, 115.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (254.52, 128.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.096 CPU sec, 6.792 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13621, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 198, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (195.28, 120.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (254.53, 128.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.994 CPU sec, 5.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13669, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 109, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (182.44, 149.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (253.08, 128.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.615 CPU sec, 5.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13846, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 154, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (151.67, 95.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (252.49, 128.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.940 CPU sec, 6.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13548, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 196, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (180.74, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (252.04, 126.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.071 CPU sec, 5.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13893, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 94, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (108.75, 54.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 220, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (253.90, 128.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.657 CPU sec, 5.309 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13616, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 101, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (125.53, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.91, 127.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.434 CPU sec, 5.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13755, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 161, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (165.59, 98.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.77, 126.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.030 CPU sec, 6.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13755, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 156, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (161.00, 91.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (250.36, 126.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.611 CPU sec, 5.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13758, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 154, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (154.47, 102.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (255.34, 129.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.734 CPU sec, 5.631 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13724, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 129, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (156.33, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (257.11, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.203 CPU sec, 6.084 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13884, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 170, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (175.76, 98.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (255.38, 127.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.856 CPU sec, 6.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13781, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 133, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (163.26, 110.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (255.16, 128.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.653 CPU sec, 5.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13767, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 182, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (195.92, 94.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.85, 129.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.242 CPU sec, 6.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13759, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 149, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (174.58, 123.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (256.42, 129.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.541 CPU sec, 5.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13634, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 138, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (166.90, 111.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 223, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (256.56, 129.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.077 CPU sec, 5.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13531, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 123, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (105.77, 42.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (255.75, 129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.061 CPU sec, 5.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13675, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 186, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (212.95, 130.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (249.79, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.000 CPU sec, 5.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13664, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 164, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (173.05, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 213, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (246.45, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.291 CPU sec, 6.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13421, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 158, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (173.83, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (244.10, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.888 CPU sec, 5.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13412, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 164, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (161.62, 102.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 213, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (245.99, 122.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.786 CPU sec, 6.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13326, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 168, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (185.42, 131.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 211, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (242.20, 120.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.622 CPU sec, 5.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13066, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 169, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (193.00, 130.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (241.25, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.229 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13195, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 159, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (170.50, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.88, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.086 CPU sec, 5.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13082, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 185, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (186.94, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 212, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (243.35, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.705 CPU sec, 5.808 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13111, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 144, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (132.06, 61.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.50, 124.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.799 CPU sec, 5.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13763, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 172, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (185.30, 104.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (254.73, 128.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.677 CPU sec, 5.342 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13558, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 141, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (158.08, 88.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 221, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (253.78, 127.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.084 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13685, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 139, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (164.93, 116.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (255.14, 128.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.221 CPU sec, 5.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13872, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 211, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (191.76, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.38, 126.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.035 CPU sec, 6.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13871, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 153, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (179.00, 112.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (248.92, 125.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.782 CPU sec, 5.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13759, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (122.41, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.52, 126.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.466 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13769, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (140.79, 104.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.60, 126.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.869 CPU sec, 5.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13925, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 173, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (190.61, 134.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.49, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.056 CPU sec, 5.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13701, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (186.00, 105.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.09, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.056 CPU sec, 5.088 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13696, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (149.82, 106.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (253.98, 127.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.462 CPU sec, 5.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13592, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 148, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (155.65, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (254.92, 126.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.622 CPU sec, 7.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13822, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 133, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (175.75, 109.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (252.50, 126.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.962 CPU sec, 5.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13649, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 210, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (195.88, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (254.37, 127.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.652 CPU sec, 5.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13703, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 223, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (216.90, 133.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (252.19, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.430 CPU sec, 5.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13642, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 120, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (126.58, 55.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 217, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (252.82, 128.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.929 CPU sec, 5.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13901, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 164, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (198.32, 106.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.77, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.848 CPU sec, 6.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13737, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (174.91, 93.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.89, 124.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.509 CPU sec, 5.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13270, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 165, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (179.88, 105.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (239.32, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.873 CPU sec, 6.023 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13670, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 179, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (164.05, 71.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (241.71, 119.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.472 CPU sec, 6.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13390, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (146.20, 83.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 205, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (237.56, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.644 CPU sec, 5.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 13234, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 124, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (152.14, 96.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (238.02, 117.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.724 CPU sec, 5.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13236, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 200, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (221.24, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 205, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (236.21, 114.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.642 CPU sec, 5.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13105, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 132, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (146.00, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (232.00, 112.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.458 CPU sec, 5.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12885, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 130, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (158.95, 85.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (232.81, 113.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.346 CPU sec, 6.230 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 13256, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 134, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (142.17, 61.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (241.91, 119.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.971 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13650, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (152.24, 68.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.98, 124.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 144.338 CPU sec, 6.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13615, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 121, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (154.10, 98.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (250.08, 123.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 152.210 CPU sec, 5.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13590, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 190, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (197.33, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (247.40, 124.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.166 CPU sec, 7.011 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13635, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 192, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (174.53, 105.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (249.34, 125.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.058 CPU sec, 5.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13679, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 167, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (188.88, 116.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 215, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (249.02, 124.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.159 CPU sec, 7.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13594, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 150, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (147.00, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (247.49, 122.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.262 CPU sec, 5.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13613, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 159, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (184.96, 133.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (245.46, 121.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.984 CPU sec, 5.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13657, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 150, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (174.00, 91.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (245.97, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.188 CPU sec, 5.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13527, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 180, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (180.96, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 220, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (250.55, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.185 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13552, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (122.00, 56.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (249.30, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.842 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13675, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 182, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (194.07, 122.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.39, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.069 CPU sec, 5.600 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 13610, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (154.53, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (250.49, 125.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.239 CPU sec, 5.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13717, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 189, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (205.20, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (249.64, 125.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.159 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13598, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 153, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (192.87, 128.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (249.98, 126.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.923 CPU sec, 5.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13558, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 184, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (177.14, 116.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (248.32, 124.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.298 CPU sec, 4.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 13698, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 150, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (180.63, 118.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (249.48, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.009 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13753, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 238, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (232.07, 162.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 213, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (246.28, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.701 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13474, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 143, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (154.78, 78.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (242.35, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.652 CPU sec, 6.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13621, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 148, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (159.90, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 209, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (240.88, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.777 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 13560, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (186.03, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (236.63, 117.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.452 CPU sec, 5.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13368, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 158, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (209.60, 145.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (236.22, 115.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.871 CPU sec, 6.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13223, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 162, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (160.68, 78.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 202, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (232.77, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.767 CPU sec, 6.096 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13071, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 149, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (146.61, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 203, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (232.40, 111.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.141 CPU sec, 6.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12972, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 183, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (186.74, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (230.06, 111.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.198 CPU sec, 6.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13536, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 129, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (159.58, 82.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (247.46, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.868 CPU sec, 5.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13595, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 149, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (188.25, 113.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.40, 126.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 138.792 CPU sec, 5.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13736, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 207, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (170.73, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.62, 125.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 147.187 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13925, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (146.18, 101.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (248.95, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 164.479 CPU sec, 6.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13720, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 132, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (132.76, 64.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (250.48, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 143.661 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13656, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 126, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (138.45, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (247.67, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.012 CPU sec, 6.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13719, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 115, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (158.83, 100.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (248.55, 124.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 145.695 CPU sec, 5.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13706, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 157, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (166.69, 107.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (248.17, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.145 CPU sec, 7.187 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13693, 8, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (173.44, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (245.57, 123.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.571 CPU sec, 5.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13681, 5, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (249.65, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 157.845 CPU sec, 5.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13738, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 111, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (123.39, 54.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (249.80, 125.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.381 CPU sec, 5.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13609, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (151.36, 91.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (251.47, 125.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.436 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 13681, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 136, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (153.15, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (250.02, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 160.968 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13733, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 174, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (171.48, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (249.62, 124.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.735 CPU sec, 5.520 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13668, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 168, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (175.05, 113.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (250.44, 125.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 140.372 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13684, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 141, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (164.38, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 220, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.70, 126.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.844 CPU sec, 5.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13712, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 101, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (92.00, 33.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.15, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.434 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13719, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 109, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (144.29, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 214, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (246.21, 122.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.747 CPU sec, 5.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13773, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 145, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (154.85, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 210, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (242.55, 120.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.821 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13333, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 239, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (212.20, 139.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 212, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (244.69, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 157.219 CPU sec, 5.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13513, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (173.11, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 206, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (238.83, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.076 CPU sec, 5.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13427, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 131, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (160.79, 95.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 209, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (239.03, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 146.771 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13259, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 146, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (159.48, 90.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (237.20, 116.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.932 CPU sec, 6.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13146, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 122, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (124.59, 56.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 205, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (236.86, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 142.730 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13038, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 177, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (187.23, 133.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 207, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (237.05, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.078 CPU sec, 6.773 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13354, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 160, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (163.19, 96.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 211, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (243.93, 121.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.111 CPU sec, 6.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13697, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 164, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (186.29, 102.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (250.34, 126.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 153.582 CPU sec, 6.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13954, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 184, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (173.57, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (250.72, 125.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 158.193 CPU sec, 7.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13594, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (157.35, 72.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (249.87, 125.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.264 CPU sec, 6.908 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13759, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 162, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (157.91, 90.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 217, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (250.20, 126.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.409 CPU sec, 5.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13800, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 206, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (195.06, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.75, 125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.835 CPU sec, 5.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13710, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 216, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (222.24, 109.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (250.42, 126.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.894 CPU sec, 5.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13439, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 182, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (179.40, 106.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (248.24, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.324 CPU sec, 6.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13679, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 146, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (160.16, 77.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 214, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (247.15, 124.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.416 CPU sec, 5.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13696, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 103, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (104.71, 56.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.93, 124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.653 CPU sec, 5.887 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13542, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 164, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (168.95, 122.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 217, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (250.25, 125.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.963 CPU sec, 5.929 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13624, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 112, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (161.88, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (248.93, 125.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 154.426 CPU sec, 5.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13712, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 181, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (202.50, 180.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (250.26, 125.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 148.722 CPU sec, 7.291 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13816, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 170, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (192.11, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (251.71, 125.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.900 CPU sec, 5.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 13630, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 141, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (167.76, 105.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 215, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (248.03, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 150.147 CPU sec, 6.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13646, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 139, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (157.64, 84.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (249.57, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 151.231 CPU sec, 5.867 real sec [M::process] read 18980 sequences (2865980 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13729, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 244, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (213.56, 120.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 219, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (251.62, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 149.141 CPU sec, 5.391 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 3967, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 218, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (252.10, 126.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 18980 reads in 41.088 CPU sec, 2.212 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21189\tSM:UG10S2P660\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21189_HLKNYBGXX_UG10S2P660_R1.fastq.gz 5347_7332_21189_HLKNYBGXX_UG10S2P660_R2.fastq.gz [main] Real time: 3520.333 sec; CPU: 91000.255 sec Finished alignement of UG10S2P660 at Wed Apr 3 23:11:22 2019 Sorting BAM file started at Wed Apr 3 23:11:22 2019 samtools sort -@ 30 -m 4G UG10S2P660_unsorted.bam -o UG10S2P660.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Wed Apr 3 23:11:22 2019 rm UG10S2P660_unsorted.bam rm 5347_7332_21189_HLKNYBGXX_UG10S2P660_R1.fastq.gz rm 5347_7332_21189_HLKNYBGXX_UG10S2P660_R2.fastq.gz samtools index UG10S2P660.bam Finished BAM file generation of the sample - UG10S2P660 Processing fastq files for the sample - UG10S2P661 Reading 5347_7332_21190_HLKNYBGXX_UG10S2P661_R1.fastq.gz 5347_7332_21190_HLKNYBGXX_UG10S2P661_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21190\tSM:UG10S2P661\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21190_HLKNYBGXX_UG10S2P661_R1.fastq.gz 5347_7332_21190_HLKNYBGXX_UG10S2P661_R2.fastq.gz | samtools view -Sb1 - > UG10S2P661_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12367, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 179, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (191.11, 93.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (243.63, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.164 CPU sec, 10.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12293, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 175, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (164.88, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (240.52, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.765 CPU sec, 9.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12247, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 128, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (141.31, 63.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.20, 123.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.779 CPU sec, 9.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12487, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 135, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (138.04, 64.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.01, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.656 CPU sec, 9.255 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12557, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 134, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (135.93, 55.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (242.03, 126.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.211 CPU sec, 8.362 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12559, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 118, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (139.85, 93.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (243.12, 125.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.496 CPU sec, 8.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12549, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (168.80, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (243.24, 127.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.118 CPU sec, 8.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12567, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (128.86, 77.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (244.16, 128.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.915 CPU sec, 8.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12583, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 108, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (159.00, 106.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.27, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.600 CPU sec, 8.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12583, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 143, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (134.76, 62.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (244.31, 127.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.698 CPU sec, 7.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12532, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 135, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (126.27, 59.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.47, 125.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.559 CPU sec, 8.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12707, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 172, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 318) [M::mem_pestat] mean and std.dev: (165.11, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.43, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.110 CPU sec, 8.632 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12757, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 166, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (185.32, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (245.52, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.896 CPU sec, 8.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12590, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 148, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (161.50, 85.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (246.31, 129.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.798 CPU sec, 7.932 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12741, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 134, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (123.65, 56.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (246.44, 127.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.236 CPU sec, 7.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12749, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 225, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (201.91, 112.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (246.64, 128.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.797 CPU sec, 9.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12729, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 180, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (194.52, 107.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (244.44, 126.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.832 CPU sec, 8.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12740, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (159.07, 105.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (244.00, 126.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.962 CPU sec, 8.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12631, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 158, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (188.33, 128.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (243.88, 127.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.561 CPU sec, 8.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12692, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 135, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (175.24, 100.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (244.27, 128.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.774 CPU sec, 8.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12713, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 118, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (130.60, 86.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (245.24, 128.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.130 CPU sec, 8.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12777, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 147, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (169.73, 111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (244.60, 128.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.108 CPU sec, 8.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12643, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 123, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (123.92, 69.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.39, 126.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.763 CPU sec, 9.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12622, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 177, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (179.86, 104.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (242.30, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.155 CPU sec, 9.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12721, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 135, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (168.91, 121.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.10, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.776 CPU sec, 8.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12822, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 155, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (167.71, 84.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.25, 124.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.159 CPU sec, 8.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12690, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 99, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (110.08, 38.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.74, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.057 CPU sec, 8.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12709, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 156, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (159.00, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (240.67, 123.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.110 CPU sec, 9.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12888, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 108, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (178.06, 113.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.91, 124.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.244 CPU sec, 8.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12599, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 181, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (214.28, 116.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.59, 123.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.646 CPU sec, 9.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12642, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 184, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (170.04, 119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.80, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.213 CPU sec, 8.679 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12610, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 225, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (195.86, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.90, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.252 CPU sec, 8.606 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12621, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 135, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (184.07, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.20, 124.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.451 CPU sec, 9.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12738, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 194, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (208.22, 118.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.26, 123.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.200 CPU sec, 8.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12516, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 127, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (148.10, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.24, 122.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 250.116 CPU sec, 10.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12608, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 185, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (154.52, 106.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.35, 123.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.023 CPU sec, 8.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12536, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 191, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (202.73, 121.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.33, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.836 CPU sec, 9.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12617, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 153, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (138.79, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (236.88, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.079 CPU sec, 8.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12707, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 196, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (186.89, 119.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (235.17, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.583 CPU sec, 7.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12621, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (123.28, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.91, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.652 CPU sec, 8.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12581, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 174, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (193.00, 127.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.60, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.603 CPU sec, 7.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12433, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (150.76, 89.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.22, 121.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.253 CPU sec, 9.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12280, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 134, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (146.38, 83.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.21, 126.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.933 CPU sec, 8.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12263, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 102, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (145.21, 95.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.47, 126.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.416 CPU sec, 8.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12292, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 159, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (187.94, 115.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.77, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.629 CPU sec, 9.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12267, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (138.31, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.99, 123.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.255 CPU sec, 9.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12213, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 140, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (120.00, 68.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.32, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.723 CPU sec, 8.003 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12492, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 157, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (156.61, 68.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (240.85, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.786 CPU sec, 8.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12426, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 177, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (159.45, 79.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.36, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.084 CPU sec, 8.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12465, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (190.29, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.35, 127.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.113 CPU sec, 8.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12546, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (147.00, 94.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (241.01, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.092 CPU sec, 8.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12560, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 109, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (144.83, 91.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.37, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.895 CPU sec, 9.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12679, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 203, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (174.74, 91.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (242.50, 125.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.639 CPU sec, 8.109 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12631, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 147, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (164.76, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.89, 126.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.287 CPU sec, 8.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12563, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 144, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (162.57, 114.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (242.68, 125.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.798 CPU sec, 8.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12895, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 123, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (119.28, 62.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.51, 127.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.149 CPU sec, 8.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12577, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 161, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (171.19, 113.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.22, 127.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.892 CPU sec, 9.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12753, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 81, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (87.83, 34.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (243.59, 127.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.954 CPU sec, 9.241 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12766, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 119, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (133.09, 73.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (243.97, 127.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.702 CPU sec, 8.533 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12645, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 149, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (174.47, 99.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.32, 127.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.183 CPU sec, 8.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12646, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 166, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (186.86, 98.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.14, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.138 CPU sec, 8.837 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12695, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 155, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (180.38, 93.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (242.74, 126.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.488 CPU sec, 9.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12836, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 128, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (157.19, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (243.14, 126.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.643 CPU sec, 8.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12720, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 154, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (176.17, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.41, 128.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.023 CPU sec, 8.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12662, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 130, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (161.19, 103.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.06, 126.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.422 CPU sec, 8.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12625, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 229, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (205.53, 131.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (242.29, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.087 CPU sec, 8.267 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12621, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 185, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (169.42, 106.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.28, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.420 CPU sec, 9.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12851, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (152.42, 92.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.91, 125.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.978 CPU sec, 8.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12547, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 179, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (217.55, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (240.12, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.709 CPU sec, 8.790 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12724, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 156, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (170.42, 86.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (242.61, 125.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.030 CPU sec, 7.972 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12567, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 177, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (200.00, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (242.02, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 204.433 CPU sec, 7.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12513, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 200, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (230.33, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (239.27, 123.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.644 CPU sec, 9.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12545, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 140, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (183.22, 132.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.92, 124.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.935 CPU sec, 9.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12675, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 144, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (160.80, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.81, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.943 CPU sec, 9.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12551, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 160, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (163.33, 92.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.27, 122.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.904 CPU sec, 9.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12812, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 119, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (177.96, 110.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.86, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 209.602 CPU sec, 9.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12574, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 120, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (157.33, 112.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.20, 118.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.935 CPU sec, 6.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12543, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 160, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (177.31, 101.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.24, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 205.179 CPU sec, 8.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12526, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 156, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (147.38, 77.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.97, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.477 CPU sec, 7.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12664, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 94, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (133.16, 94.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.26, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.876 CPU sec, 8.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12465, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 141, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (159.10, 133.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.78, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.041 CPU sec, 6.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12606, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 148, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (166.92, 124.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.70, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.860 CPU sec, 6.976 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12470, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 148, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (176.00, 97.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.21, 121.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.158 CPU sec, 8.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12582, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 171, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (181.76, 124.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.21, 122.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.618 CPU sec, 7.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12634, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 139, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (154.59, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.41, 120.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.889 CPU sec, 9.257 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12421, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 156, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (145.56, 57.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (244.68, 126.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.724 CPU sec, 8.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12363, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 183, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (202.10, 140.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (244.11, 126.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.841 CPU sec, 8.623 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12388, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 156, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (174.83, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (242.22, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.284 CPU sec, 8.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12258, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 163, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (173.85, 91.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.71, 124.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.172 CPU sec, 8.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12403, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 150, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (176.15, 108.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.39, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.209 CPU sec, 7.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12431, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (149.10, 122.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (239.85, 124.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.973 CPU sec, 7.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12419, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 139, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (176.86, 119.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.47, 125.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.607 CPU sec, 8.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12485, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 126, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (141.50, 89.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (244.09, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.475 CPU sec, 8.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12433, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (164.47, 88.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (242.50, 125.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.538 CPU sec, 8.448 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12410, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 132, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (160.36, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.05, 126.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.582 CPU sec, 8.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12537, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (149.24, 89.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.50, 125.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.486 CPU sec, 9.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12736, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 138, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (167.22, 114.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.03, 126.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.064 CPU sec, 8.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12695, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 196, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (193.92, 98.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.31, 126.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.151 CPU sec, 8.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12692, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 103, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (180.80, 128.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (239.69, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.355 CPU sec, 8.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12535, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 173, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (183.44, 107.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (244.92, 127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.209 CPU sec, 8.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12681, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (170.38, 117.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.00, 126.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.177 CPU sec, 8.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12702, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 156, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (192.50, 128.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.41, 126.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.187 CPU sec, 9.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12432, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 163, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (179.95, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (243.14, 125.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.665 CPU sec, 8.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12726, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 171, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (187.88, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (243.14, 126.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.415 CPU sec, 9.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12749, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 171, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (171.50, 114.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.95, 125.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.877 CPU sec, 8.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12709, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 143, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (151.00, 85.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (242.83, 126.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.551 CPU sec, 8.513 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12612, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 148, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (189.52, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (240.72, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.276 CPU sec, 8.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12591, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 133, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (161.88, 98.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.87, 124.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.083 CPU sec, 8.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12628, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 228, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (187.12, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.63, 124.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.180 CPU sec, 8.412 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12607, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 112, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (168.52, 112.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.78, 126.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.410 CPU sec, 8.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12633, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (196.11, 87.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.10, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.203 CPU sec, 8.737 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12549, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 146, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (174.62, 86.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.40, 124.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.086 CPU sec, 8.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12718, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (138.57, 73.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.68, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.861 CPU sec, 8.882 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12634, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 151, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (150.07, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (241.49, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.963 CPU sec, 8.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12552, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 207, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (157.20, 82.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.77, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.206 CPU sec, 8.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12472, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 190, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (205.72, 139.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (235.81, 122.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.145 CPU sec, 9.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12557, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 148, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (156.86, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (233.58, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.202 CPU sec, 8.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12544, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (172.80, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.30, 121.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.049 CPU sec, 8.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12474, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 144, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (160.61, 100.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.59, 120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.489 CPU sec, 7.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12423, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 140, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (143.05, 117.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.87, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.739 CPU sec, 8.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12289, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (124.69, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.60, 120.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.278 CPU sec, 8.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12485, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 160, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (196.21, 160.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (234.81, 121.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.100 CPU sec, 7.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12503, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 148, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (142.14, 74.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.40, 120.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.733 CPU sec, 8.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12494, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 167, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (178.48, 92.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.49, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.114 CPU sec, 9.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12411, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 129, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (160.44, 106.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.96, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.299 CPU sec, 8.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12364, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 179, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (180.62, 139.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (233.75, 119.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.368 CPU sec, 7.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12454, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 145, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (164.58, 86.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.14, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.807 CPU sec, 8.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12439, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 150, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (147.10, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.32, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.056 CPU sec, 9.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12337, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 113, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (120.47, 68.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.02, 120.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.592 CPU sec, 8.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12250, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 138, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (165.94, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.12, 120.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.324 CPU sec, 7.319 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12354, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (162.71, 108.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.11, 120.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.267 CPU sec, 8.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12401, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 119, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (162.35, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.12, 122.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.683 CPU sec, 8.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 144, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (174.26, 104.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.79, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.175 CPU sec, 8.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12553, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 199, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (182.10, 113.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.92, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.160 CPU sec, 7.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12568, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 197, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (203.38, 142.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (241.25, 126.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.981 CPU sec, 9.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12598, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 123, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (125.68, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.90, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.919 CPU sec, 8.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12628, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 146, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (154.06, 95.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (238.12, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.589 CPU sec, 7.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12716, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 168, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (170.70, 93.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.39, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.690 CPU sec, 7.749 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12684, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 163, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (178.33, 86.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.86, 124.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.238 CPU sec, 8.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12777, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 160, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (159.19, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (242.22, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.345 CPU sec, 9.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12736, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 161, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (206.54, 142.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.38, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.819 CPU sec, 7.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12734, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 130, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (143.37, 93.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (242.43, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.310 CPU sec, 7.860 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12463, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (187.82, 164.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.62, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.878 CPU sec, 8.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12817, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 109, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (126.00, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (242.33, 125.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.084 CPU sec, 8.703 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12779, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 171, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (212.00, 146.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (241.00, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.256 CPU sec, 8.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12716, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 161, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (161.12, 103.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.48, 125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.960 CPU sec, 8.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12567, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 125, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (158.41, 104.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.70, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.268 CPU sec, 8.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12639, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 143, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (199.91, 129.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.65, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.156 CPU sec, 8.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12650, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 148, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (174.50, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.06, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.851 CPU sec, 8.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12661, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 188, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (194.42, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.91, 124.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.999 CPU sec, 8.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12615, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 177, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (188.05, 98.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.55, 123.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.716 CPU sec, 8.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12648, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 130, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (156.26, 96.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.06, 123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.200 CPU sec, 8.525 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12653, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 154, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (166.26, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.09, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.482 CPU sec, 8.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12584, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 169, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (163.94, 104.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.27, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.173 CPU sec, 8.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12665, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 164, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (155.19, 76.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.78, 122.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.043 CPU sec, 8.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12596, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 169, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (177.10, 108.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.58, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.333 CPU sec, 8.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12676, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 122, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (125.50, 59.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.20, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.915 CPU sec, 10.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12560, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 143, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (159.64, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.79, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 214.550 CPU sec, 7.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12613, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (160.00, 101.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (234.53, 119.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.901 CPU sec, 8.381 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12510, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 187, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (203.04, 118.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (234.99, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.763 CPU sec, 9.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12767, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 213, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (192.33, 144.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (234.99, 120.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.277 CPU sec, 8.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12440, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 182, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (198.83, 117.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (235.83, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.127 CPU sec, 8.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12552, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 163, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (230.58, 140.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.04, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.746 CPU sec, 8.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12809, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 102, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (124.12, 78.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.15, 119.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.357 CPU sec, 7.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12511, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 157, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (147.42, 56.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.64, 120.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.800 CPU sec, 8.822 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12507, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 96, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (137.29, 79.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.30, 120.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.263 CPU sec, 7.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12477, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 150, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (160.85, 103.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.80, 117.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.275 CPU sec, 9.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12472, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 135, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (144.86, 93.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (231.81, 118.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.949 CPU sec, 8.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12532, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 141, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (164.92, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.21, 119.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.837 CPU sec, 8.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12418, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 171, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (142.71, 102.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (232.17, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.407 CPU sec, 8.082 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12258, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 107, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (132.33, 87.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.60, 118.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.479 CPU sec, 8.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12211, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 235, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (203.13, 113.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (232.82, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.240 CPU sec, 7.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12334, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 93, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (142.65, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.01, 120.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.372 CPU sec, 8.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12189, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 144, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (154.62, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (235.99, 121.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 249.440 CPU sec, 9.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12295, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (152.62, 108.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (235.88, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.269 CPU sec, 8.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12447, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 168, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (157.22, 109.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.44, 121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.073 CPU sec, 8.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12435, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 167, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (173.70, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (235.95, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.457 CPU sec, 8.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12500, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 198, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 405) [M::mem_pestat] mean and std.dev: (190.00, 68.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.23, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.030 CPU sec, 9.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12490, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (114.57, 46.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.45, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.486 CPU sec, 9.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12637, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 170, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (181.62, 92.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.87, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.013 CPU sec, 8.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12497, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 201, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (217.22, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.77, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.238 CPU sec, 9.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12660, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 185, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (241.10, 173.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.59, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.233 CPU sec, 8.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12684, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 146, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (161.67, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (239.77, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.354 CPU sec, 8.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12785, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 158, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (193.18, 134.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.36, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.636 CPU sec, 9.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12509, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 97, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (89.93, 39.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.14, 123.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.796 CPU sec, 8.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12757, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 182, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (192.46, 114.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.20, 124.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.684 CPU sec, 8.821 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12809, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (137.88, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.76, 121.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.406 CPU sec, 8.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12591, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (176.15, 131.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (238.26, 122.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.489 CPU sec, 7.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12634, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 160, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (175.87, 92.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.89, 125.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.796 CPU sec, 9.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12567, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 222, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (192.92, 107.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.67, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.774 CPU sec, 8.037 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12585, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 167, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (169.85, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.82, 124.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.495 CPU sec, 9.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12554, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 170, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (189.22, 102.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.27, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.693 CPU sec, 8.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12769, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 191, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (204.29, 89.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.87, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.819 CPU sec, 8.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12779, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 200, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (190.33, 108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (236.89, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.158 CPU sec, 8.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12599, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 167, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (152.13, 63.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (239.52, 124.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.178 CPU sec, 9.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12647, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 110, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (136.50, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (239.39, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.828 CPU sec, 9.027 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12547, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 225, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (200.50, 118.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.85, 122.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.422 CPU sec, 8.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12545, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 203, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (197.79, 122.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (237.63, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.318 CPU sec, 8.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12717, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 164, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (165.60, 62.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.42, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.657 CPU sec, 8.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12589, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (128.66, 88.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.75, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.166 CPU sec, 9.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12517, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 186, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (176.67, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.48, 118.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.618 CPU sec, 9.894 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12771, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 170, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (184.07, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (234.42, 118.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.355 CPU sec, 8.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12614, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 160, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (183.94, 108.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.58, 120.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.505 CPU sec, 8.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12504, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 150, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (180.00, 125.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (233.86, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.694 CPU sec, 9.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12575, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 156, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (168.29, 100.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.85, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.253 CPU sec, 8.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12421, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 190, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (145.80, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.63, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.819 CPU sec, 8.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12421, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 188, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (191.17, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.86, 119.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.614 CPU sec, 9.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12520, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 154, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (182.79, 140.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.30, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.683 CPU sec, 9.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12467, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 116, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (154.61, 106.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.41, 118.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.580 CPU sec, 9.568 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12302, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 137, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (154.46, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.19, 119.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.613 CPU sec, 8.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12660, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (127.81, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.36, 119.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.812 CPU sec, 8.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12524, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 138, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (153.64, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.73, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.067 CPU sec, 8.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12456, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 124, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (136.56, 89.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.33, 117.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.949 CPU sec, 9.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12212, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 187, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (196.96, 115.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (235.55, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.180 CPU sec, 9.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12208, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 184, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (181.56, 132.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.55, 119.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.203 CPU sec, 8.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12326, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 136, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (144.24, 79.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.22, 119.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.141 CPU sec, 9.811 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12208, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 187, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (177.60, 116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (235.38, 120.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.186 CPU sec, 8.549 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12375, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 152, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (177.82, 109.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.75, 122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.073 CPU sec, 8.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12525, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 144, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (186.84, 132.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (237.84, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.501 CPU sec, 9.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12389, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 129, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (169.44, 95.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.01, 123.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.454 CPU sec, 8.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12626, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 150, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (183.11, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.25, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.117 CPU sec, 9.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12555, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 125, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (164.35, 115.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.79, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.398 CPU sec, 8.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12569, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 175, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (196.33, 106.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.17, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.772 CPU sec, 8.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12679, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 155, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (170.94, 99.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.53, 123.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.987 CPU sec, 8.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12516, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 195, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (221.72, 103.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.72, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.736 CPU sec, 9.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12542, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (233.30, 160.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.94, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.979 CPU sec, 9.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12686, 6, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (237.68, 124.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 239.656 CPU sec, 8.220 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12604, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 174, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (179.95, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.10, 123.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.098 CPU sec, 9.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12604, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 193, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 346) [M::mem_pestat] mean and std.dev: (188.14, 48.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.94, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.932 CPU sec, 8.624 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12666, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 170, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (201.06, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (240.42, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.894 CPU sec, 8.312 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12571, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 139, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (127.36, 47.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (239.78, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.309 CPU sec, 9.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12577, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 154, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (150.28, 88.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.88, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.393 CPU sec, 8.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12555, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 152, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (137.10, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.79, 121.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.367 CPU sec, 9.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12685, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 160, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (179.84, 126.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.98, 122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.429 CPU sec, 7.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12560, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 118, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (161.28, 118.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (239.01, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.412 CPU sec, 8.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12456, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 235, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (227.95, 131.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.75, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.868 CPU sec, 8.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12580, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 130, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (182.55, 130.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.37, 121.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.358 CPU sec, 8.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12555, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 134, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (141.58, 78.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.29, 121.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.685 CPU sec, 9.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12522, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 150, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (160.20, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (236.32, 121.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.812 CPU sec, 8.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12566, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (132.56, 83.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (234.19, 121.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.254 CPU sec, 8.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12466, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 245, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (205.27, 102.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.08, 121.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.713 CPU sec, 7.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12463, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 202, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (194.04, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (234.72, 120.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 256.053 CPU sec, 9.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12461, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 120, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (124.71, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.94, 119.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.785 CPU sec, 8.266 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12394, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 139, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (176.35, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (231.70, 119.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.381 CPU sec, 7.803 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12551, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 124, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (150.87, 91.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.75, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.325 CPU sec, 8.842 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12470, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 168, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (172.18, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (232.69, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.198 CPU sec, 8.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12347, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 149, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (145.60, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.36, 119.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.130 CPU sec, 9.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12420, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 164, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (184.71, 106.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.69, 118.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.269 CPU sec, 8.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12406, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 131, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (171.72, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (233.24, 120.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.189 CPU sec, 8.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12278, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 170, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (149.04, 76.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (229.88, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.047 CPU sec, 7.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12419, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 193, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (190.77, 99.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.27, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.490 CPU sec, 9.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12336, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 99, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (115.95, 76.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (232.13, 118.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.425 CPU sec, 10.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12351, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (126.80, 68.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.97, 119.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.017 CPU sec, 8.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12378, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 158, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (187.09, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.46, 119.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.887 CPU sec, 8.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12548, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 135, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (141.00, 64.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.29, 120.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.177 CPU sec, 9.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12650, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 174, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (188.82, 104.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (243.71, 126.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.484 CPU sec, 8.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12582, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 141, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (155.56, 80.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.67, 126.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.256 CPU sec, 8.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12696, 4, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (159.29, 127.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (246.14, 127.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.317 CPU sec, 8.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12803, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 141, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (147.75, 95.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.21, 126.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.546 CPU sec, 8.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12638, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 221, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (229.90, 134.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.87, 125.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.101 CPU sec, 8.252 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12531, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 116, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (168.64, 113.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (242.69, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.334 CPU sec, 9.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12744, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 161, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (201.71, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (242.35, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.844 CPU sec, 7.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12619, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 149, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (144.76, 62.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.42, 125.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.276 CPU sec, 7.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12605, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 179, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (201.08, 130.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (243.33, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.303 CPU sec, 8.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12558, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 159, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (153.19, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (243.89, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.007 CPU sec, 8.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12595, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (168.14, 77.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.58, 125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.505 CPU sec, 8.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12642, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 176, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (180.19, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.26, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.782 CPU sec, 7.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12638, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 159, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (142.50, 52.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (243.82, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.483 CPU sec, 8.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12660, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 165, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (154.40, 95.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.30, 126.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.679 CPU sec, 7.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12633, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 144, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (184.44, 132.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.04, 126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.362 CPU sec, 8.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12643, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 246, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (242.75, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.92, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.817 CPU sec, 10.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12408, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 152, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (143.96, 83.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.41, 127.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.897 CPU sec, 8.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12503, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 171, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (164.38, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.54, 126.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.394 CPU sec, 8.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12715, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 203, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (187.60, 129.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.08, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.429 CPU sec, 7.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12854, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 126, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (141.33, 76.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (242.02, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.451 CPU sec, 9.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12789, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 127, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (153.80, 99.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.58, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.835 CPU sec, 8.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12655, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 146, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (176.16, 104.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (242.00, 126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.823 CPU sec, 8.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12515, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 165, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (182.64, 103.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.80, 124.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.941 CPU sec, 8.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12546, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 134, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (139.53, 113.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.84, 127.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.913 CPU sec, 8.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12660, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 138, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (138.77, 60.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.88, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.800 CPU sec, 7.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12525, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 143, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (173.12, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (240.73, 124.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.784 CPU sec, 8.975 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12660, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (196.83, 110.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (242.38, 126.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.587 CPU sec, 8.868 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12480, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 157, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (157.20, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (236.45, 122.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.744 CPU sec, 7.847 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12667, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 111, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (138.67, 85.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.89, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.416 CPU sec, 8.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12558, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 131, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (162.24, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (237.99, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.433 CPU sec, 8.144 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12540, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 188, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (174.68, 93.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.57, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.802 CPU sec, 8.251 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12278, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 160, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (185.71, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.26, 120.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.442 CPU sec, 8.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12432, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 166, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (178.25, 106.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.45, 120.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.720 CPU sec, 9.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12269, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 150, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (166.76, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.59, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.361 CPU sec, 8.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12291, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 185, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (162.54, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.65, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.976 CPU sec, 9.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12283, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 151, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (154.85, 79.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.91, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.803 CPU sec, 9.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12134, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 142, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (152.00, 98.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.14, 120.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.192 CPU sec, 10.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12097, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 177, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (195.62, 101.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.98, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.345 CPU sec, 9.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12046, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 121, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (138.68, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.74, 117.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.142 CPU sec, 8.938 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 11993, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (125.67, 75.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (228.71, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.949 CPU sec, 9.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11839, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 141, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (141.24, 102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (229.82, 116.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.647 CPU sec, 9.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11822, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 149, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (175.40, 93.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.48, 118.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.084 CPU sec, 9.985 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12295, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 156, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (167.53, 101.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.95, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.242 CPU sec, 8.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12715, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 241, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 434) [M::mem_pestat] mean and std.dev: (231.13, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.78, 129.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.487 CPU sec, 8.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12598, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 128, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (170.00, 86.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (247.44, 130.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.608 CPU sec, 7.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12771, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 147, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (177.60, 133.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.37, 127.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.461 CPU sec, 8.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12456, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 158, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (158.16, 79.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (243.82, 126.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.264 CPU sec, 8.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12661, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 184, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (180.00, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (246.69, 127.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.266 CPU sec, 8.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12709, 8, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (115.85, 71.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (245.73, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.121 CPU sec, 8.205 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12527, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 140, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (166.12, 92.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 210, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (247.09, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.526 CPU sec, 9.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12740, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 129, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (134.05, 61.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (248.08, 129.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.007 CPU sec, 8.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12733, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 182, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (181.80, 96.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (245.39, 126.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.094 CPU sec, 8.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12727, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 239, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (197.73, 79.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (244.72, 127.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.570 CPU sec, 8.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12720, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 189, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (211.55, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (245.23, 127.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.705 CPU sec, 9.036 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12673, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (190.67, 99.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (247.82, 128.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.119 CPU sec, 8.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12738, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (166.75, 74.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (244.71, 127.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.280 CPU sec, 8.665 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12805, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 113, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (159.59, 100.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (244.44, 126.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.340 CPU sec, 7.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12594, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 138, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (185.07, 124.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (245.41, 127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.373 CPU sec, 8.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12924, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (121.90, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (244.40, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 220.829 CPU sec, 7.923 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12619, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 175, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (185.65, 89.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.08, 126.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.381 CPU sec, 8.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12705, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 113, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (151.09, 95.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (246.62, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.662 CPU sec, 8.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12699, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 191, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (201.87, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (246.48, 128.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.655 CPU sec, 8.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12678, 9, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (164.79, 114.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.60, 127.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.338 CPU sec, 8.132 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12678, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 149, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (130.70, 59.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (243.25, 127.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.708 CPU sec, 8.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12813, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 253, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (226.47, 95.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (245.39, 128.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.832 CPU sec, 8.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12701, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 183, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (198.25, 128.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (241.93, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.978 CPU sec, 9.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12674, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 172, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (154.61, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (243.68, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.372 CPU sec, 9.718 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12657, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 172, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (206.95, 140.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.31, 126.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.248 CPU sec, 8.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12683, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 147, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (164.89, 103.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (244.41, 127.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.083 CPU sec, 9.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12571, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 177, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (193.43, 131.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.62, 123.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.327 CPU sec, 8.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12704, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 228, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (211.15, 118.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.57, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.340 CPU sec, 8.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12624, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 183, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (190.58, 125.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.51, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.178 CPU sec, 8.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12557, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (150.05, 70.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (239.05, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.570 CPU sec, 7.983 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12498, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 160, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (169.10, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (235.68, 121.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.863 CPU sec, 8.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12453, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 172, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (168.00, 86.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.72, 120.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.781 CPU sec, 8.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12379, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 158, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (175.23, 107.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (232.17, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.852 CPU sec, 8.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12321, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 126, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (169.14, 118.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.59, 119.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.003 CPU sec, 8.014 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12354, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 131, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (137.61, 61.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.03, 117.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.417 CPU sec, 9.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12306, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 142, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (151.27, 98.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.27, 118.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.187 CPU sec, 9.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12348, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 94, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (125.19, 66.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.55, 117.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.921 CPU sec, 7.721 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12155, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 156, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (182.68, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (230.76, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.960 CPU sec, 9.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12081, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 159, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (166.77, 101.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.05, 116.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.400 CPU sec, 8.960 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 11861, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 131, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (144.62, 67.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.10, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.911 CPU sec, 9.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11841, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 161, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (219.05, 143.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.07, 120.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 251.800 CPU sec, 8.941 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11990, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 138, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (204.43, 148.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.46, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.852 CPU sec, 8.676 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12332, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 134, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (150.52, 95.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.62, 127.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.987 CPU sec, 7.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12608, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (208.28, 109.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (248.67, 128.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.881 CPU sec, 8.638 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12625, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 145, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (188.95, 111.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (248.20, 128.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.014 CPU sec, 8.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12733, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 132, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (157.68, 83.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (248.23, 128.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.843 CPU sec, 8.099 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12653, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 162, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (160.89, 81.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (247.20, 128.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.850 CPU sec, 8.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12591, 4, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 178, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (179.38, 109.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.40, 128.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.137 CPU sec, 8.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12684, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 163, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (206.58, 134.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (247.90, 128.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.621 CPU sec, 9.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12593, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 154, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (184.44, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.95, 128.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.685 CPU sec, 9.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12624, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 153, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (164.16, 103.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 211, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.94, 126.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.356 CPU sec, 8.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12617, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 132, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (164.72, 105.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (246.42, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.520 CPU sec, 9.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12589, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 176, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (188.32, 101.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.44, 128.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.135 CPU sec, 8.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12649, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 98, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (148.13, 104.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (246.03, 127.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.554 CPU sec, 8.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12625, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (169.59, 116.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (245.87, 127.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.862 CPU sec, 8.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12736, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 133, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (138.59, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (245.39, 127.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.093 CPU sec, 9.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12614, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 132, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (154.07, 101.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.00, 127.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.185 CPU sec, 8.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12699, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 149, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (136.47, 77.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 211, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (246.08, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.041 CPU sec, 8.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12703, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 203, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (201.71, 142.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (244.97, 127.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.285 CPU sec, 8.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12636, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 201, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (200.38, 108.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (247.55, 128.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.775 CPU sec, 8.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12726, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 183, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (207.30, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (243.72, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.196 CPU sec, 8.699 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12593, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 214, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (152.33, 92.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.68, 126.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.251 CPU sec, 9.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12506, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 125, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (154.00, 88.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.37, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 252.033 CPU sec, 8.918 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12663, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 118, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (131.12, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.00, 126.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.274 CPU sec, 7.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12611, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 166, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (219.06, 184.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.31, 126.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.509 CPU sec, 8.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12729, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 171, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (193.44, 89.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (242.77, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.389 CPU sec, 9.206 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12730, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.15, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 229.176 CPU sec, 8.006 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12727, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 192, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (185.96, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (241.73, 125.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.380 CPU sec, 7.991 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12544, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 213, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (221.00, 93.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (238.89, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.287 CPU sec, 7.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12632, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 222, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (219.94, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.76, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.973 CPU sec, 8.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12609, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (194.33, 100.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (238.03, 123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.581 CPU sec, 9.996 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12488, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 166, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (176.43, 85.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (236.46, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.515 CPU sec, 9.046 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12434, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 145, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (157.93, 104.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.33, 119.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.408 CPU sec, 8.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12512, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 171, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (185.95, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.13, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.184 CPU sec, 9.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12507, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 151, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (188.25, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (233.77, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.044 CPU sec, 8.035 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12501, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (146.78, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (231.87, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.336 CPU sec, 8.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12434, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 156, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (160.81, 83.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.86, 117.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.877 CPU sec, 8.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12254, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 164, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (192.70, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (232.12, 119.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.353 CPU sec, 9.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12129, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 133, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (169.07, 104.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.22, 117.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.235 CPU sec, 8.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12133, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 216, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (210.40, 118.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.14, 115.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.767 CPU sec, 9.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12206, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 167, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (156.59, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.13, 116.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.823 CPU sec, 8.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12060, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 154, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (165.54, 87.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.12, 116.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 215.692 CPU sec, 7.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11968, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 122, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (157.88, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (230.20, 117.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.930 CPU sec, 8.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11683, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 154, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (177.62, 101.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (230.98, 118.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 258.923 CPU sec, 9.778 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12074, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 147, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (147.92, 68.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.53, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 254.284 CPU sec, 9.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12720, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 172, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (183.00, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.36, 124.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.425 CPU sec, 9.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12643, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 136, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (153.27, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (237.49, 121.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.932 CPU sec, 8.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12652, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 123, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (130.91, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.26, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.418 CPU sec, 10.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12434, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 144, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (169.85, 117.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.03, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.699 CPU sec, 9.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12651, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 174, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (191.64, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.53, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.550 CPU sec, 8.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12617, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (166.41, 99.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.41, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.765 CPU sec, 9.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12560, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 198, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (188.77, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.53, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.185 CPU sec, 9.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12608, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 123, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (153.86, 87.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (240.07, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.950 CPU sec, 8.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12451, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 177, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (208.71, 157.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (239.81, 124.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.571 CPU sec, 8.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12663, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 167, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (157.96, 61.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.43, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.819 CPU sec, 9.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12587, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 204, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (198.47, 91.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.20, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.548 CPU sec, 9.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12649, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 135, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (192.00, 147.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.62, 124.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 231.069 CPU sec, 9.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12639, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 194, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (201.00, 121.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.63, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.454 CPU sec, 8.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12526, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 203, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (185.92, 96.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.02, 123.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.209 CPU sec, 8.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 12547, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.75, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 234.186 CPU sec, 8.613 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12603, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 110, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (186.00, 175.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (241.04, 124.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.060 CPU sec, 7.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12723, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 196, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (188.61, 75.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.34, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.130 CPU sec, 8.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12423, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 113, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (119.79, 77.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.82, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.294 CPU sec, 8.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12818, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (156.65, 109.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (238.90, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.741 CPU sec, 8.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12598, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 142, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (150.16, 99.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (239.90, 123.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.328 CPU sec, 8.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12599, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 115, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (145.72, 76.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.39, 125.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 253.492 CPU sec, 9.375 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12793, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (128.42, 63.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.87, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 219.644 CPU sec, 8.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12697, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 190, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (191.57, 102.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.27, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.936 CPU sec, 8.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12483, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 221, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (203.15, 134.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.35, 123.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 244.392 CPU sec, 9.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12579, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 190, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (183.55, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.06, 122.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.042 CPU sec, 8.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12558, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 133, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (179.18, 107.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.87, 120.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.196 CPU sec, 8.228 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12535, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 166, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (170.17, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.14, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.329 CPU sec, 8.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12475, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 160, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (170.62, 105.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.13, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 242.866 CPU sec, 8.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12544, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 154, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (159.29, 98.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (233.23, 120.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 245.052 CPU sec, 8.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12230, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (186.28, 112.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.42, 119.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.270 CPU sec, 8.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12241, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 163, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (152.05, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.88, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 248.732 CPU sec, 10.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12370, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 191, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (187.17, 107.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.41, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.496 CPU sec, 8.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12434, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 148, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (155.05, 81.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.05, 119.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.027 CPU sec, 7.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12411, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 146, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (171.00, 148.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (229.30, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.514 CPU sec, 9.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12343, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (208.69, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (229.16, 117.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.019 CPU sec, 8.506 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12227, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 134, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (145.86, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (227.99, 115.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.345 CPU sec, 8.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12326, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (150.73, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (228.31, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.734 CPU sec, 8.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12260, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 221, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (171.85, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (226.87, 114.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.764 CPU sec, 8.495 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12144, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 148, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (160.83, 90.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (226.83, 114.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.523 CPU sec, 8.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12101, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 166, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (189.80, 93.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (225.51, 112.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 247.577 CPU sec, 9.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11908, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 176, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (192.05, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (225.13, 112.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 241.935 CPU sec, 8.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11847, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (156.81, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (226.84, 113.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 246.396 CPU sec, 8.875 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12482, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (111.81, 59.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.37, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.432 CPU sec, 10.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12622, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 250, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (236.33, 170.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.39, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.468 CPU sec, 8.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12547, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 165, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (153.47, 77.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.15, 125.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.958 CPU sec, 7.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12573, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 188, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (200.23, 104.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.89, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.487 CPU sec, 7.776 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12570, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 156, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (186.63, 107.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.91, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.789 CPU sec, 7.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12645, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 148, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (160.58, 107.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (243.72, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.166 CPU sec, 8.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12783, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 134, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (189.18, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (243.28, 127.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.052 CPU sec, 8.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12626, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 160, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (148.33, 62.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (242.18, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.785 CPU sec, 8.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12726, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (139.29, 70.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (243.92, 127.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.185 CPU sec, 8.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12576, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 165, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (167.22, 84.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 208, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (242.48, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.105 CPU sec, 8.893 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12557, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 104, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (107.67, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.71, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.617 CPU sec, 8.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12756, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 196, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (202.71, 110.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.14, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 223.711 CPU sec, 8.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12791, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (149.48, 88.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.32, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.450 CPU sec, 10.337 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12675, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 135, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (146.62, 87.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.67, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.366 CPU sec, 9.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12692, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 184, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (169.75, 71.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (241.06, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.142 CPU sec, 7.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12756, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 128, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (158.05, 91.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.25, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.475 CPU sec, 8.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12716, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 145, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (180.44, 120.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (242.43, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 224.741 CPU sec, 7.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12559, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 148, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (157.06, 107.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (243.49, 126.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 216.235 CPU sec, 7.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12515, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 118, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (173.75, 130.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.01, 127.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 217.290 CPU sec, 7.739 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12716, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 145, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (172.38, 119.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.50, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.335 CPU sec, 8.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12702, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 131, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (168.53, 104.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (242.05, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 237.700 CPU sec, 8.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12473, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 168, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (209.00, 155.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (242.97, 125.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 238.063 CPU sec, 8.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12449, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 147, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (165.14, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.77, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.394 CPU sec, 10.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12682, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 227, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (210.77, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.77, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.691 CPU sec, 8.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12588, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 151, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (168.09, 116.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.98, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 227.542 CPU sec, 8.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12577, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 177, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (186.38, 118.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (238.42, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 229.010 CPU sec, 8.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12670, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (138.48, 104.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.76, 122.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.329 CPU sec, 8.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12743, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 154, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (195.58, 118.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.74, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 226.187 CPU sec, 8.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12663, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 201, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (208.08, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.03, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 236.857 CPU sec, 8.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12447, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 175, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (161.46, 70.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.55, 120.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.611 CPU sec, 7.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12479, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 144, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (195.38, 141.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (232.14, 118.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.018 CPU sec, 8.076 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12425, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 164, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (179.33, 91.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (231.91, 118.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 235.238 CPU sec, 8.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12482, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 161, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (181.46, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.28, 116.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.490 CPU sec, 8.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12414, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (149.87, 87.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (228.96, 116.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 234.910 CPU sec, 9.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12208, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (154.23, 92.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (227.56, 115.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 243.430 CPU sec, 9.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12210, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 177, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (193.20, 105.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.27, 115.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 240.434 CPU sec, 8.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12149, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 125, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (154.81, 96.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.56, 115.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 198.598 CPU sec, 6.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12027, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 156, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (163.33, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (226.59, 114.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.780 CPU sec, 6.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11830, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 218, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (248.47, 215.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (226.69, 113.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.682 CPU sec, 6.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11834, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 107, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (165.10, 134.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (223.21, 111.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.221 CPU sec, 7.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11686, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 173, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (189.72, 104.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (224.18, 113.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.685 CPU sec, 7.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11788, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 193, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (167.22, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (225.99, 113.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 201.094 CPU sec, 7.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12504, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 96, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (145.06, 113.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.70, 125.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 218.533 CPU sec, 7.855 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12651, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 188, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (150.89, 88.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.41, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 225.083 CPU sec, 8.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12762, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 157, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (189.95, 116.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (243.51, 126.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 230.239 CPU sec, 8.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12642, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (118.67, 62.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (244.21, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.670 CPU sec, 8.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12538, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 137, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (144.17, 78.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.96, 125.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.613 CPU sec, 7.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12527, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 155, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (165.83, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (243.01, 126.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 207.604 CPU sec, 7.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12513, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 144, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (160.60, 83.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (243.07, 126.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.110 CPU sec, 7.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12706, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 214, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (217.35, 112.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.62, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 210.740 CPU sec, 7.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12747, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 179, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (190.59, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (242.64, 125.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 222.076 CPU sec, 8.359 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12530, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 125, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (130.74, 56.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (244.87, 127.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.464 CPU sec, 7.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12469, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 141, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (145.60, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.48, 124.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.360 CPU sec, 7.333 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12600, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 137, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (209.23, 165.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.85, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 211.020 CPU sec, 7.279 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12805, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 186, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (226.50, 149.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.34, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 221.645 CPU sec, 7.797 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12722, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 227, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (217.82, 117.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.96, 127.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 228.876 CPU sec, 8.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12786, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (124.21, 78.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (241.05, 125.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 239.485 CPU sec, 8.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12521, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (144.44, 69.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (244.82, 128.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.015 CPU sec, 8.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12696, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 128, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (131.86, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.37, 125.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 232.011 CPU sec, 8.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12530, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 207, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (180.36, 102.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.68, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 233.093 CPU sec, 8.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12694, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 138, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (125.00, 49.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.51, 128.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.567 CPU sec, 6.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12540, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 139, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (150.48, 94.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.17, 125.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.398 CPU sec, 7.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12661, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 178, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (177.32, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.97, 124.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.199 CPU sec, 6.981 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12674, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 163, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (164.67, 80.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.16, 124.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.847 CPU sec, 8.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12680, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 231, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (220.44, 111.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.18, 122.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.850 CPU sec, 6.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12655, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 160, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (174.76, 108.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.34, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.760 CPU sec, 6.278 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12777, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 200, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (188.48, 108.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (241.07, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.515 CPU sec, 6.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12719, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 159, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (180.31, 129.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (236.89, 122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.263 CPU sec, 7.065 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12567, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 104, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (169.17, 138.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (234.54, 119.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.131 CPU sec, 6.835 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12575, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 176, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (194.31, 99.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.84, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.919 CPU sec, 7.166 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12585, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 161, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (173.16, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 197, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (232.02, 120.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.136 CPU sec, 7.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12236, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 146, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (191.08, 140.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.19, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.344 CPU sec, 6.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12484, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 141, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (160.41, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.93, 118.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.933 CPU sec, 6.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12308, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 158, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (152.37, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.92, 118.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.489 CPU sec, 6.327 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12410, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 218, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (214.65, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.99, 119.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 163.324 CPU sec, 6.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12357, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 104, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (111.70, 54.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.18, 117.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.421 CPU sec, 6.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12292, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (161.42, 98.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.64, 116.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.351 CPU sec, 6.743 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12218, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (184.73, 134.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.06, 114.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.848 CPU sec, 6.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12039, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 144, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (158.00, 102.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (224.97, 112.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.424 CPU sec, 6.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12085, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 153, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (178.03, 113.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (225.60, 114.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.409 CPU sec, 8.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12081, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 182, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (202.50, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 191, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (224.07, 113.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.875 CPU sec, 6.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 11709, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 183, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (210.38, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 191, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (224.66, 112.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.805 CPU sec, 7.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11740, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 151, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (197.75, 118.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (222.31, 110.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.285 CPU sec, 5.741 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 11558, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 189, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (201.50, 111.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (225.74, 113.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.119 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12605, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 168, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (176.54, 75.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.27, 121.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.691 CPU sec, 6.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12490, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 140, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (176.20, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (236.50, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.649 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12509, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 121, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (136.67, 87.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.08, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 159.226 CPU sec, 5.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12505, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 119, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (144.13, 78.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.25, 120.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 155.681 CPU sec, 6.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12570, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 144, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (182.61, 137.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.18, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 156.363 CPU sec, 5.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12553, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 110, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (152.75, 103.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.21, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.913 CPU sec, 7.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12469, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 168, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (173.48, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (232.89, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 161.110 CPU sec, 6.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12421, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 174, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (177.19, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.81, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.262 CPU sec, 6.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12561, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 134, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (172.07, 109.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (233.91, 120.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 162.804 CPU sec, 6.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12596, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 143, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (161.59, 77.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (230.59, 117.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.132 CPU sec, 7.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12430, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (158.52, 108.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.63, 118.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.477 CPU sec, 6.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12484, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 155, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (188.88, 116.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (231.27, 119.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.287 CPU sec, 7.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12595, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 189, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (175.91, 106.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.97, 125.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.939 CPU sec, 6.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12820, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 136, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (153.41, 86.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.91, 126.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.096 CPU sec, 6.397 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12803, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 211, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (224.67, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.23, 123.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.640 CPU sec, 7.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12506, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (220.58, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.94, 125.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.449 CPU sec, 6.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12781, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 149, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (167.88, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (242.53, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.855 CPU sec, 7.045 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12712, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (124.69, 70.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (242.80, 125.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.499 CPU sec, 6.366 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12587, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 148, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (171.00, 146.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.40, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.719 CPU sec, 6.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12784, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 162, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (204.54, 153.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (240.08, 124.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.005 CPU sec, 6.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12622, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 125, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (135.95, 81.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.60, 125.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.000 CPU sec, 7.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12659, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (165.22, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (241.37, 124.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.870 CPU sec, 5.987 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12658, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (127.26, 80.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.12, 125.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.456 CPU sec, 6.696 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12495, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 104, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (148.80, 88.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.34, 125.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.371 CPU sec, 6.993 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12585, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (115.76, 62.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.14, 123.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.503 CPU sec, 6.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12619, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 127, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (155.63, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.98, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.951 CPU sec, 6.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12556, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 128, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (152.90, 94.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.65, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.731 CPU sec, 6.453 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12149, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 177, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (182.74, 107.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.06, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.540 CPU sec, 6.760 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12195, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 130, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (159.25, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.96, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.307 CPU sec, 7.722 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12390, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 121, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (160.19, 93.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.86, 122.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.100 CPU sec, 7.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12286, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 174, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (196.86, 132.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.87, 124.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.191 CPU sec, 7.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12488, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 156, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (164.28, 96.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (239.68, 125.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.024 CPU sec, 6.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12538, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 199, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (180.08, 109.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.76, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.417 CPU sec, 5.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12446, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 183, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (200.59, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (238.98, 123.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.965 CPU sec, 6.733 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12610, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 185, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (188.50, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (238.63, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.929 CPU sec, 6.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12598, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 132, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (139.67, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.93, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.558 CPU sec, 6.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12541, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (215.45, 166.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.15, 125.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.619 CPU sec, 6.441 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12561, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 191, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (200.29, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (240.22, 124.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.678 CPU sec, 6.848 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12571, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 177, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (171.80, 105.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.88, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.526 CPU sec, 6.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12528, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 143, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (156.40, 98.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (240.72, 125.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.765 CPU sec, 7.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12495, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 201, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (186.07, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.24, 124.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.017 CPU sec, 6.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12726, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 121, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (120.36, 49.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.55, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.563 CPU sec, 6.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12480, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (165.11, 113.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.46, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.144 CPU sec, 6.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12619, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 153, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (167.00, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.66, 121.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.353 CPU sec, 7.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12580, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 167, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (153.91, 63.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.25, 120.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.864 CPU sec, 7.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12607, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 165, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (180.86, 106.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.40, 120.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.857 CPU sec, 7.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12422, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 163, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (183.27, 107.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.35, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.589 CPU sec, 6.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12386, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 201, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (202.90, 112.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.50, 120.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.403 CPU sec, 7.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12433, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 158, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (172.37, 105.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.67, 121.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.430 CPU sec, 6.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12630, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 154, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (163.00, 91.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (233.65, 119.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.698 CPU sec, 6.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12470, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (146.57, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.23, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.130 CPU sec, 6.083 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12418, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 185, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (170.15, 99.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.73, 119.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.724 CPU sec, 7.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12509, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 233, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (217.52, 126.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.01, 119.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.273 CPU sec, 6.818 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12615, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (121.15, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (238.49, 122.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.738 CPU sec, 6.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12552, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 115, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (151.04, 99.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.31, 125.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.674 CPU sec, 7.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12927, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 168, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (176.93, 103.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (242.45, 126.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.949 CPU sec, 6.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12630, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 134, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (162.48, 100.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (244.03, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.358 CPU sec, 7.427 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12805, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (160.39, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.24, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.224 CPU sec, 7.029 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12676, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 152, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (177.00, 113.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (240.84, 125.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.675 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12688, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 216, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (253.29, 159.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (240.47, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.765 CPU sec, 6.774 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12593, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (165.95, 113.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (240.78, 125.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.168 CPU sec, 6.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12571, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 160, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (165.05, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.92, 125.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.799 CPU sec, 6.817 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12822, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 118, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (163.50, 122.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.72, 124.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.869 CPU sec, 6.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12891, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 193, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (200.85, 115.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.95, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.315 CPU sec, 5.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12589, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 111, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (164.53, 108.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (243.66, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.576 CPU sec, 6.105 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12659, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 164, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (218.78, 140.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (239.03, 123.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.026 CPU sec, 6.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12696, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 167, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (352.47, 321.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.54, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.419 CPU sec, 6.151 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12591, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 155, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (164.12, 102.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (240.72, 124.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.578 CPU sec, 6.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12260, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 167, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (185.21, 106.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.17, 126.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.630 CPU sec, 7.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12273, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 178, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (179.44, 98.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.26, 125.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.047 CPU sec, 7.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12231, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 161, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (172.63, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.39, 122.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.996 CPU sec, 6.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12255, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 161, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (172.04, 90.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (236.97, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.059 CPU sec, 7.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12387, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 80, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (79.83, 40.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.35, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.655 CPU sec, 5.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12384, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 202, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (189.00, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.57, 123.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.811 CPU sec, 7.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12662, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 112, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (149.46, 102.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.99, 121.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.873 CPU sec, 5.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12593, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 118, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (153.50, 105.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.79, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.963 CPU sec, 7.348 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12564, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 212, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (216.00, 94.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (239.29, 123.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.295 CPU sec, 7.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12584, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 126, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (165.30, 86.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.10, 124.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.387 CPU sec, 6.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12586, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (171.56, 74.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.34, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.152 CPU sec, 7.160 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12593, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 152, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (162.05, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.53, 125.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.024 CPU sec, 7.593 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12634, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 124, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (138.89, 81.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.51, 125.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.001 CPU sec, 6.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12547, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 148, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (175.28, 133.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.09, 126.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.164 CPU sec, 6.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12654, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 126, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (147.43, 68.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.52, 124.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.322 CPU sec, 6.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12503, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 161, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (158.56, 93.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.35, 120.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.557 CPU sec, 6.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12555, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 130, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (139.95, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (233.92, 119.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.862 CPU sec, 6.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12678, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 173, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (181.11, 128.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.63, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.680 CPU sec, 6.653 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12697, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 135, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (175.62, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.13, 121.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.509 CPU sec, 7.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12253, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 155, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (194.05, 120.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.30, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.718 CPU sec, 6.269 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12529, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (143.43, 86.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.01, 118.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.194 CPU sec, 7.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12510, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 160, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (154.78, 95.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (227.67, 115.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.091 CPU sec, 6.385 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12438, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 184, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (191.59, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.71, 115.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.127 CPU sec, 6.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12538, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 114, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (126.00, 74.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (229.66, 117.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.884 CPU sec, 6.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12510, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 153, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (195.36, 130.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (230.48, 116.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.888 CPU sec, 6.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12327, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 169, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (177.47, 81.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (228.29, 115.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.132 CPU sec, 6.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12514, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 164, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (162.96, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (227.90, 115.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.489 CPU sec, 7.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12689, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 214, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (199.24, 112.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (243.90, 127.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.055 CPU sec, 6.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12809, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 170, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (183.75, 88.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.15, 127.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.431 CPU sec, 7.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12781, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 320, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (249.91, 139.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.97, 126.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.511 CPU sec, 7.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12599, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 233, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (279.15, 177.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (242.03, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.663 CPU sec, 6.707 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12697, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 108, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (136.56, 70.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.28, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.030 CPU sec, 6.177 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12678, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 194, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (199.68, 114.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.60, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.082 CPU sec, 7.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12649, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 138, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (182.40, 142.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (242.14, 125.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.383 CPU sec, 5.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12790, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 169, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (183.79, 91.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.53, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.431 CPU sec, 7.514 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12610, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 180, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (175.35, 100.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (238.44, 123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.341 CPU sec, 6.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12529, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (146.71, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.57, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.490 CPU sec, 6.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12603, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 161, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (171.88, 115.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (235.05, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.328 CPU sec, 6.982 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12508, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 156, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (167.91, 95.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (234.99, 120.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.547 CPU sec, 7.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12684, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 155, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (151.44, 86.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.74, 120.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.796 CPU sec, 5.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12571, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 219, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (231.82, 89.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.46, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.911 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12147, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 98, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (135.19, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (239.98, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.942 CPU sec, 7.117 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12290, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 121, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (141.62, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.54, 122.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.116 CPU sec, 7.670 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12252, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 178, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (189.05, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.42, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.723 CPU sec, 6.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12339, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 142, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (169.83, 116.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.17, 123.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.288 CPU sec, 6.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12459, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 185, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (190.08, 95.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (240.69, 125.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.617 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12612, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 155, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (159.83, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.71, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.022 CPU sec, 6.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12454, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 175, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (223.26, 126.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.54, 125.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.308 CPU sec, 6.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12460, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 101, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (163.12, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.21, 127.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.965 CPU sec, 6.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12761, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 153, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (151.78, 80.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.06, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.036 CPU sec, 6.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12636, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 143, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (147.65, 66.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.90, 123.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.913 CPU sec, 6.430 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12611, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 189, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (173.23, 89.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (242.05, 124.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.924 CPU sec, 7.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12698, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 220, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (188.56, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.83, 125.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.449 CPU sec, 6.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12760, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 263, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (229.15, 126.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (242.84, 126.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.675 CPU sec, 5.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12729, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 159, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (154.23, 79.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.56, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.163 CPU sec, 6.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12831, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 141, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 239) [M::mem_pestat] mean and std.dev: (133.44, 31.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (240.04, 124.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.428 CPU sec, 6.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 12336, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 157, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (171.38, 88.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (227.43, 115.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.104 CPU sec, 6.826 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12325, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 218, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (198.70, 105.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (234.42, 120.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.365 CPU sec, 7.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12564, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 168, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (153.38, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.32, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.974 CPU sec, 6.876 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12497, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (117.26, 63.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.09, 119.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.047 CPU sec, 7.853 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12516, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 187, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (180.76, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (233.11, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.957 CPU sec, 7.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12623, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 162, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (176.00, 99.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (230.20, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.165 CPU sec, 7.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12538, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 209, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (193.91, 109.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.03, 118.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.561 CPU sec, 7.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12394, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 184, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (184.89, 96.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.75, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.565 CPU sec, 6.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12394, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (144.19, 83.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.48, 117.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.290 CPU sec, 6.511 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12289, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 129, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (129.54, 76.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.95, 117.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.921 CPU sec, 6.978 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12293, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 179, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (178.78, 80.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.84, 117.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.716 CPU sec, 6.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12378, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 156, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (180.08, 111.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (231.29, 117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.579 CPU sec, 6.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12762, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (189.45, 127.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (241.54, 124.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.066 CPU sec, 6.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12513, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (129.50, 69.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (239.68, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.343 CPU sec, 7.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12605, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 220, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (209.73, 103.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (236.90, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.857 CPU sec, 7.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12739, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 149, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (191.40, 102.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.63, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.172 CPU sec, 6.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12541, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 207, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (181.45, 87.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (237.91, 122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.067 CPU sec, 7.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12615, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 162, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (181.95, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.52, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.800 CPU sec, 7.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12547, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 124, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (175.28, 127.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (239.58, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.796 CPU sec, 7.651 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12655, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 156, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (147.65, 55.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (238.54, 124.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.834 CPU sec, 6.236 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12520, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 125, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (138.50, 72.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (237.54, 122.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.446 CPU sec, 7.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12674, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 184, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (185.83, 87.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.46, 122.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.868 CPU sec, 8.025 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12710, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (153.00, 92.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.46, 120.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.969 CPU sec, 6.274 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12532, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 158, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (159.86, 95.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (234.92, 120.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.145 CPU sec, 6.865 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12697, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 120, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (143.77, 81.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (236.08, 121.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.435 CPU sec, 6.210 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12504, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (161.07, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.84, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.643 CPU sec, 8.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12253, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (134.73, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (232.43, 119.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.779 CPU sec, 6.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12081, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 167, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (201.70, 148.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (230.55, 117.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.517 CPU sec, 7.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12066, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 172, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (158.38, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.11, 118.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.707 CPU sec, 6.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12188, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 147, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (179.00, 113.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (230.98, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.648 CPU sec, 7.358 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12321, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 181, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (206.31, 123.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (232.52, 118.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.679 CPU sec, 7.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12469, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 115, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (148.50, 90.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.43, 119.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.482 CPU sec, 6.371 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12516, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 208, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (207.50, 132.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.37, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.559 CPU sec, 6.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12589, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (131.29, 69.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.61, 121.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.108 CPU sec, 7.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12489, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 291, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (257.44, 147.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.36, 121.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.246 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12546, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 119, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (177.05, 122.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.63, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.069 CPU sec, 6.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12653, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 188, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (187.00, 103.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (235.38, 120.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.248 CPU sec, 7.049 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12566, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 108, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (159.07, 185.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.02, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.034 CPU sec, 7.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12674, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 157, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (164.16, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (239.09, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.842 CPU sec, 6.951 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12677, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 192, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (195.25, 92.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (235.62, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.811 CPU sec, 6.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12713, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 96, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (135.45, 96.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (234.61, 119.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.538 CPU sec, 7.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12571, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 202, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (193.37, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.06, 119.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.813 CPU sec, 6.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12495, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 177, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (165.69, 93.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 203, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.87, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.841 CPU sec, 6.732 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12592, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 162, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (182.14, 102.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (233.74, 119.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.016 CPU sec, 6.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12520, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 200, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (167.16, 102.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.98, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.730 CPU sec, 5.970 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12446, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 120, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (157.95, 94.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.38, 120.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.884 CPU sec, 8.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12415, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (141.33, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.90, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.318 CPU sec, 7.428 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12633, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 152, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (183.14, 110.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (232.11, 116.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.317 CPU sec, 7.910 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12361, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 171, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (181.77, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.38, 117.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.643 CPU sec, 6.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12322, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 116, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (133.32, 79.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.69, 117.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.638 CPU sec, 7.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12489, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 104, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (138.04, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (231.69, 117.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.883 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12565, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (184.85, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.47, 119.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 196.626 CPU sec, 7.907 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12366, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 169, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (192.13, 129.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.84, 119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.704 CPU sec, 7.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12597, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 179, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (187.14, 99.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (239.03, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.397 CPU sec, 6.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12712, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 117, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (133.89, 64.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.38, 124.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.733 CPU sec, 6.284 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12729, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 141, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (165.45, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.64, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.936 CPU sec, 6.454 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12682, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 169, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (171.56, 84.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.67, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.855 CPU sec, 6.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12648, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 172, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (142.70, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.22, 122.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.131 CPU sec, 6.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12654, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 78, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (134.00, 113.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.92, 121.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.426 CPU sec, 6.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12566, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 173, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (169.71, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.29, 122.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.934 CPU sec, 6.408 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12578, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 149, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (168.71, 69.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.88, 120.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.199 CPU sec, 7.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12648, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (223.46, 112.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.36, 121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.586 CPU sec, 6.769 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12617, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 232, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (190.06, 109.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (235.73, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.850 CPU sec, 6.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12545, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 160, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (148.29, 83.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.11, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.851 CPU sec, 6.505 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12565, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 166, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (183.88, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.10, 120.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.879 CPU sec, 6.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12591, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 193, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (149.38, 77.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (236.78, 123.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.322 CPU sec, 6.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12545, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 153, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (176.58, 103.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.80, 121.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.048 CPU sec, 6.216 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12233, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 148, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (158.17, 76.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (227.39, 115.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.163 CPU sec, 6.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12186, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 168, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (144.12, 76.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.03, 119.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.474 CPU sec, 6.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12369, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 192, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (195.42, 128.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (231.30, 117.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.879 CPU sec, 6.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12346, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 172, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (177.30, 100.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (232.26, 119.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.055 CPU sec, 6.246 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12234, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (110.79, 67.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (234.94, 119.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.692 CPU sec, 6.786 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12387, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 149, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (149.31, 64.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (233.96, 119.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.385 CPU sec, 6.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12417, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 146, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (150.14, 76.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.08, 119.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.156 CPU sec, 7.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12445, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 192, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (187.73, 104.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.14, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.136 CPU sec, 6.825 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12526, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 175, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (171.72, 67.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (235.92, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.087 CPU sec, 7.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12488, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 147, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (141.19, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.16, 119.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.409 CPU sec, 6.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12461, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (169.79, 105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.53, 120.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.482 CPU sec, 6.729 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12547, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 181, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (195.16, 78.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (236.89, 121.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.168 CPU sec, 6.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12352, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 155, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (144.08, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.30, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.974 CPU sec, 6.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12553, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (159.28, 109.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.10, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.589 CPU sec, 7.130 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12793, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 213, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (191.19, 115.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (235.88, 121.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.991 CPU sec, 6.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12531, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 130, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (138.78, 86.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (228.60, 117.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.477 CPU sec, 6.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12615, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 190, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (211.14, 129.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (231.55, 117.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.970 CPU sec, 5.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12358, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 132, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (164.35, 126.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.08, 116.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 170.445 CPU sec, 6.197 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12643, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 90, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (143.00, 103.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.23, 117.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.043 CPU sec, 6.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12339, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 140, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (135.19, 69.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.80, 116.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.453 CPU sec, 6.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12623, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 140, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (158.52, 107.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (230.87, 118.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.945 CPU sec, 7.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12466, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 129, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (155.27, 92.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 194, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (228.54, 116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.623 CPU sec, 6.736 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12375, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 163, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (164.76, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.25, 116.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.739 CPU sec, 6.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12430, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 154, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (158.48, 92.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (230.59, 116.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.890 CPU sec, 7.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12337, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 127, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (168.00, 103.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (228.84, 115.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.977 CPU sec, 7.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12489, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 144, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (177.21, 125.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (228.89, 115.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.238 CPU sec, 7.218 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12445, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 206, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (238.23, 173.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (229.74, 116.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.124 CPU sec, 6.294 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12516, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 109, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (157.64, 130.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.47, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.437 CPU sec, 6.688 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12708, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 149, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (177.39, 106.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.68, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.634 CPU sec, 6.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12575, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 167, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (176.00, 78.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (237.65, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.198 CPU sec, 6.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12597, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (146.53, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.65, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.485 CPU sec, 6.661 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12556, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (155.06, 116.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.42, 121.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.705 CPU sec, 6.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12671, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 168, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (156.33, 87.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.29, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.405 CPU sec, 6.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12465, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 122, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (204.70, 157.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.62, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.374 CPU sec, 7.365 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12546, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 161, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (150.92, 106.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.64, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.243 CPU sec, 7.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12493, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 188, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (198.59, 108.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.65, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.365 CPU sec, 7.077 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12583, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 126, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (155.23, 122.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (235.08, 122.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.612 CPU sec, 6.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12508, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 233, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (193.43, 112.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.37, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.936 CPU sec, 7.300 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12610, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 150, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (156.10, 84.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (231.56, 119.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.127 CPU sec, 7.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12494, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 161, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (161.08, 93.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.66, 120.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.464 CPU sec, 6.455 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12462, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (126.85, 49.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (231.76, 118.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.600 CPU sec, 6.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12122, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 133, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (154.60, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.24, 121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.095 CPU sec, 6.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12287, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 216, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (195.92, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.25, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.562 CPU sec, 6.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12174, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 157, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (158.36, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.47, 120.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.114 CPU sec, 7.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12420, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 152, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (164.69, 105.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (230.65, 119.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.131 CPU sec, 6.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12355, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 131, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (149.50, 87.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.34, 119.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.504 CPU sec, 6.086 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12405, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 129, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (135.08, 69.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.96, 120.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.379 CPU sec, 6.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12560, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 166, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (180.72, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (234.87, 121.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.014 CPU sec, 7.018 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12389, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 182, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (176.59, 102.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.01, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.404 CPU sec, 6.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12592, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 189, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (188.52, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (234.87, 121.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.409 CPU sec, 7.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12505, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 121, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (147.46, 76.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.78, 121.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.719 CPU sec, 7.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12521, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 122, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (102.57, 49.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.40, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.947 CPU sec, 6.470 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12527, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 130, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (159.95, 96.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.93, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.467 CPU sec, 6.819 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12679, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (172.68, 126.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (239.93, 124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.429 CPU sec, 6.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12640, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 141, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (153.05, 88.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.43, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.116 CPU sec, 6.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12606, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 120, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (129.79, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.67, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.392 CPU sec, 6.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12455, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 123, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (129.14, 61.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (231.90, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.701 CPU sec, 7.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12415, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 165, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (181.47, 94.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.09, 116.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.350 CPU sec, 6.800 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12330, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 117, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (142.47, 83.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.15, 116.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.105 CPU sec, 6.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12255, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 198, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (194.20, 93.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.48, 117.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.171 CPU sec, 6.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12271, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 151, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (154.52, 76.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (230.83, 118.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.632 CPU sec, 6.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12290, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 123, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (140.50, 95.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (227.64, 114.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.470 CPU sec, 6.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12131, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 148, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (175.75, 103.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (226.78, 114.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.221 CPU sec, 7.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12032, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 164, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (172.55, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (225.94, 113.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.035 CPU sec, 6.967 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11927, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 103, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (128.20, 90.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.34, 117.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.155 CPU sec, 6.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11879, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (121.25, 80.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (227.91, 114.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.664 CPU sec, 7.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11986, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 177, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (35, 325) [M::mem_pestat] mean and std.dev: (171.94, 57.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.52, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.100 CPU sec, 5.841 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11783, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 135, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (163.36, 100.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (231.78, 118.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.666 CPU sec, 6.863 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12222, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 153, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (181.00, 110.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.38, 121.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.948 CPU sec, 7.402 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12711, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 176, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (202.47, 131.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.45, 124.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.839 CPU sec, 7.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12420, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 142, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (161.44, 92.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.28, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.844 CPU sec, 6.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12552, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (137.96, 61.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.24, 125.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.948 CPU sec, 7.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12604, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 163, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (175.69, 96.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (237.55, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.571 CPU sec, 5.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12703, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 120, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (151.35, 84.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (238.85, 122.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.861 CPU sec, 5.934 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12671, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 128, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (114.12, 39.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.15, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.915 CPU sec, 6.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12740, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 143, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (145.59, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.84, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.771 CPU sec, 6.814 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12699, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 104, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (150.13, 108.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.21, 122.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.930 CPU sec, 7.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 12654, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.53, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 172.392 CPU sec, 5.942 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12899, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 145, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (147.32, 82.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (237.86, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.923 CPU sec, 6.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12472, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 200, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (204.44, 147.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (239.37, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.437 CPU sec, 6.899 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12619, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 141, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (170.39, 99.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.19, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.632 CPU sec, 7.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12581, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 164, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (186.05, 113.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.62, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.839 CPU sec, 7.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12415, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 193, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (186.65, 100.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.67, 122.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.764 CPU sec, 6.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12685, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 162, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (164.03, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (243.32, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.882 CPU sec, 6.450 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12603, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 196, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (197.39, 120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.00, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.378 CPU sec, 6.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12380, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (152.27, 98.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (242.58, 125.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.425 CPU sec, 6.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12680, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 145, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (156.52, 113.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (242.62, 125.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.863 CPU sec, 7.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 12718, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 164, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (167.52, 88.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.26, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.645 CPU sec, 6.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12613, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 150, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (150.15, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (243.73, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.781 CPU sec, 6.149 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12507, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 131, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (172.00, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.96, 126.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.649 CPU sec, 6.636 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12674, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 160, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (154.31, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.71, 124.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.212 CPU sec, 7.662 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12615, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 193, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (211.07, 139.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (240.57, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.329 CPU sec, 6.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12508, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 175, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (168.44, 112.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.70, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.761 CPU sec, 6.858 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12620, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (228.47, 127.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.46, 124.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.627 CPU sec, 6.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12652, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 106, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (114.47, 69.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (241.13, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.328 CPU sec, 6.619 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12658, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 122, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (146.25, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.95, 123.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.077 CPU sec, 7.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12611, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 150, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (146.17, 63.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.61, 125.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.743 CPU sec, 6.645 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12411, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 116, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (144.41, 83.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (233.97, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.813 CPU sec, 6.916 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12490, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 156, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (179.00, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (236.53, 121.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.615 CPU sec, 6.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12480, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 99, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (133.14, 89.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (231.24, 118.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.440 CPU sec, 7.745 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12163, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 168, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (155.11, 85.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (232.34, 120.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.839 CPU sec, 7.357 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12298, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 105, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (138.40, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.63, 118.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.475 CPU sec, 6.215 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12353, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 124, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (153.61, 86.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (229.79, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.050 CPU sec, 7.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12291, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 181, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (188.05, 83.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (228.42, 116.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.852 CPU sec, 7.112 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12117, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 154, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (166.71, 86.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.69, 116.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.970 CPU sec, 6.781 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 11790, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 214, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (214.47, 127.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (228.14, 114.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.941 CPU sec, 6.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 11976, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 169, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (176.83, 89.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.41, 115.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.038 CPU sec, 7.121 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11974, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 164, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (173.48, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (230.63, 116.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.727 CPU sec, 6.576 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 11904, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 104, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (125.71, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.74, 120.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.655 CPU sec, 7.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12077, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 144, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (157.70, 77.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.36, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.836 CPU sec, 6.584 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12793, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 120, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (145.50, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.86, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.047 CPU sec, 7.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12764, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 161, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (168.06, 100.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (245.51, 128.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.721 CPU sec, 6.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12696, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 129, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (173.35, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (243.52, 125.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.236 CPU sec, 6.372 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12755, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (151.05, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.87, 127.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.081 CPU sec, 6.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12555, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 205, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (209.00, 129.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (239.91, 125.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.335 CPU sec, 6.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12609, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 131, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (138.06, 95.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (239.58, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.694 CPU sec, 6.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12704, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 136, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (157.62, 100.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.22, 124.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.911 CPU sec, 5.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12732, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 227, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (235.07, 148.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.35, 124.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.754 CPU sec, 6.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12702, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 169, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (156.54, 64.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.31, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.944 CPU sec, 7.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12585, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 145, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (154.75, 88.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.05, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.660 CPU sec, 6.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12713, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 167, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (169.74, 91.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (237.90, 124.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.182 CPU sec, 6.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12678, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 165, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (193.79, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.02, 122.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.304 CPU sec, 7.101 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12636, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 116, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (114.63, 70.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.87, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.934 CPU sec, 6.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12456, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 146, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (177.05, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.83, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.336 CPU sec, 6.145 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12556, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (154.86, 108.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (241.39, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.159 CPU sec, 7.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12664, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 243, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (220.28, 136.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (244.12, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.366 CPU sec, 6.627 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12737, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 143, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (163.06, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 209, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (246.34, 128.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.755 CPU sec, 6.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12652, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (181.56, 129.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (244.93, 128.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.430 CPU sec, 6.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12625, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (205.44, 140.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.42, 125.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.730 CPU sec, 5.955 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12568, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 207, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (194.62, 100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.04, 124.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.378 CPU sec, 6.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12651, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 234, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (205.81, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 210, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (246.34, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.603 CPU sec, 6.204 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12705, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 137, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (134.75, 76.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.96, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.661 CPU sec, 5.915 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12578, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 203, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (198.24, 95.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 209, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (244.90, 126.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.757 CPU sec, 6.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 12566, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 125, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (130.33, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (243.66, 126.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.912 CPU sec, 6.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12513, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 160, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (180.35, 123.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (242.14, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.987 CPU sec, 6.226 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12642, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 153, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (165.06, 78.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.76, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.519 CPU sec, 6.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12695, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 166, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (175.07, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.32, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.536 CPU sec, 6.235 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12698, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 154, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (164.79, 86.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (240.01, 123.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.200 CPU sec, 6.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12648, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (111.96, 52.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (234.75, 119.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.120 CPU sec, 6.959 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12382, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 112, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (136.32, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.71, 121.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.995 CPU sec, 6.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12585, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 143, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (178.52, 115.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (232.96, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.777 CPU sec, 6.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12514, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 116, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (159.90, 88.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.28, 119.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.790 CPU sec, 5.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12295, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 194, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (212.42, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.62, 119.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.230 CPU sec, 7.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12515, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 204, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (227.22, 136.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (232.72, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.551 CPU sec, 6.831 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12448, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 176, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (170.12, 83.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.22, 117.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.614 CPU sec, 5.974 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12178, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 144, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (167.31, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.76, 117.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.866 CPU sec, 7.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11916, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 165, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (173.90, 93.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.68, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.133 CPU sec, 7.949 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12166, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 175, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (207.30, 114.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (230.08, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.891 CPU sec, 6.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12002, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 143, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (172.12, 101.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.29, 119.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.218 CPU sec, 6.178 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11867, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 185, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (185.47, 96.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.21, 119.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.067 CPU sec, 6.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 11857, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 109, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (137.31, 84.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.26, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.930 CPU sec, 6.364 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12715, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 140, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (163.70, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (243.84, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.033 CPU sec, 6.072 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12647, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 154, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (169.55, 111.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.06, 124.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.973 CPU sec, 6.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12644, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 172, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (178.30, 108.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (242.39, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 189.467 CPU sec, 7.221 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12664, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 146, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (163.68, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.47, 125.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.321 CPU sec, 6.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12700, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 119, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (173.93, 99.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (243.04, 126.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.329 CPU sec, 7.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12703, 0, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 166, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (174.17, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (241.09, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.581 CPU sec, 5.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12734, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 140, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (172.57, 97.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (241.42, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.659 CPU sec, 6.058 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12535, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (141.78, 102.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (239.13, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.833 CPU sec, 6.452 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12775, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 176, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (203.07, 111.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (240.62, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.990 CPU sec, 6.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12497, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 117, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (117.00, 64.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.98, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.373 CPU sec, 7.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12537, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 138, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (165.80, 103.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.14, 122.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.688 CPU sec, 7.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12678, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 214, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (195.20, 102.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (238.82, 125.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.390 CPU sec, 6.335 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12758, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 162, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (203.86, 133.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.42, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.592 CPU sec, 6.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12627, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 112, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (156.71, 127.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (238.71, 122.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.049 CPU sec, 6.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12806, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 153, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (140.50, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.22, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.831 CPU sec, 6.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12688, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 107, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (186.58, 175.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (241.67, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.289 CPU sec, 6.720 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12676, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 153, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (210.41, 158.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.71, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.684 CPU sec, 6.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12732, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 149, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (166.25, 101.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (242.59, 125.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.690 CPU sec, 6.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12887, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 226, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (220.29, 130.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (240.03, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.943 CPU sec, 6.392 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12561, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 144, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (145.38, 63.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (242.13, 124.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.113 CPU sec, 6.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12689, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 199, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (204.46, 90.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (243.28, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.832 CPU sec, 6.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12651, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 190, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (172.15, 81.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 208, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (242.86, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.592 CPU sec, 6.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12698, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 166, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (188.19, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.87, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.330 CPU sec, 6.239 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12552, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 179, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (202.05, 119.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (241.13, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.882 CPU sec, 6.159 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12750, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 166, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (178.67, 115.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 207, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (242.86, 125.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.696 CPU sec, 6.200 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12575, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (172.89, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.09, 124.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.908 CPU sec, 6.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12574, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 173, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (168.27, 89.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (240.82, 124.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.570 CPU sec, 6.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12695, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 166, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (209.69, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.69, 122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.171 CPU sec, 7.122 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12087, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 110, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (124.76, 60.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (223.98, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.949 CPU sec, 7.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12462, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 126, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (152.32, 112.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (230.07, 117.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.567 CPU sec, 6.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12338, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 91, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (104.71, 60.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.04, 115.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.324 CPU sec, 6.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12352, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 122, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (135.21, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.18, 115.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.599 CPU sec, 7.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12390, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (134.88, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (225.42, 113.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.473 CPU sec, 7.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12340, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 153, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (148.41, 74.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (225.56, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.432 CPU sec, 6.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12251, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 156, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (191.56, 131.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (224.07, 113.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.941 CPU sec, 6.551 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12149, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 154, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (184.08, 119.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (224.82, 112.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.976 CPU sec, 6.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12045, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (164.25, 91.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 188, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (221.56, 110.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.382 CPU sec, 7.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 11858, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (145.69, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (223.38, 110.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.139 CPU sec, 6.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 11759, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (156.06, 105.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (218.25, 107.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.835 CPU sec, 6.465 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 11759, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 178, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (167.82, 84.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (223.80, 111.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.515 CPU sec, 6.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12105, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 190, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (185.64, 113.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (229.52, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.749 CPU sec, 6.586 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12568, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 129, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (162.00, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.68, 121.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.889 CPU sec, 6.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12746, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 143, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (144.00, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.48, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.430 CPU sec, 6.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12488, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (108.44, 56.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.59, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.930 CPU sec, 7.253 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12615, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 122, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (155.26, 104.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.54, 123.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.618 CPU sec, 6.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12440, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (163.05, 126.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.91, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.346 CPU sec, 6.605 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12546, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 163, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (171.95, 92.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.00, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.722 CPU sec, 6.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12637, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (189.09, 106.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.03, 122.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.431 CPU sec, 6.589 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12649, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 178, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (166.60, 89.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.21, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.622 CPU sec, 6.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12673, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 161, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (167.94, 107.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.94, 119.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.354 CPU sec, 6.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12679, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 155, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (176.45, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.79, 121.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.261 CPU sec, 7.940 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12380, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 100, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (120.00, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.86, 119.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.229 CPU sec, 6.466 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12539, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 119, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (140.38, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.59, 120.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.902 CPU sec, 7.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12478, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 151, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (184.35, 101.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (231.72, 118.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.968 CPU sec, 7.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12636, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 170, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (171.79, 84.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (234.89, 120.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.267 CPU sec, 7.717 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12577, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 148, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (140.24, 66.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.43, 122.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.679 CPU sec, 6.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12511, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 207, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (177.14, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.92, 123.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.831 CPU sec, 6.124 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12494, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 196, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (186.26, 93.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (236.91, 121.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.558 CPU sec, 7.001 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12543, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 224, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (216.31, 133.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.59, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.966 CPU sec, 6.107 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12468, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 135, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (155.78, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.13, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.468 CPU sec, 7.481 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12567, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 199, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (206.04, 109.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.73, 122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.198 CPU sec, 6.118 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12539, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 180, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (175.94, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.65, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.729 CPU sec, 6.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12520, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 150, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (172.07, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.28, 123.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.637 CPU sec, 6.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12644, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 189, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (183.83, 118.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.53, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.940 CPU sec, 6.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12644, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 148, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (179.80, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.32, 122.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.650 CPU sec, 6.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12523, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 153, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (158.92, 101.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.66, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.540 CPU sec, 6.610 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12613, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 157, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (165.26, 93.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.12, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.271 CPU sec, 6.990 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12701, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 192, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (213.76, 130.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (239.16, 124.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.870 CPU sec, 5.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12515, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 114, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (151.10, 92.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (229.65, 118.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.716 CPU sec, 7.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12403, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 147, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (182.59, 99.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (228.41, 116.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 208.729 CPU sec, 7.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12613, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 194, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (188.06, 103.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (228.83, 115.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.224 CPU sec, 7.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12490, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 160, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (198.00, 125.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.76, 116.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 190.218 CPU sec, 6.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12333, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 160, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (155.15, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (227.39, 115.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.075 CPU sec, 6.283 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12403, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 155, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (168.04, 102.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (223.91, 112.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.362 CPU sec, 6.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 12208, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 142, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (163.78, 93.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (224.80, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 166.287 CPU sec, 6.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12154, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 111, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (136.71, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (220.47, 109.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.530 CPU sec, 5.999 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12000, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 141, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (158.09, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 188, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (219.72, 109.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.496 CPU sec, 6.141 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 11982, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 206, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (194.78, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (220.96, 109.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 195.153 CPU sec, 7.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 11858, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 147, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (163.80, 101.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (220.16, 109.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 191.952 CPU sec, 6.772 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11778, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (158.67, 107.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 189, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (221.72, 110.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.223 CPU sec, 6.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 11681, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 147, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (143.81, 89.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (221.28, 110.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.109 CPU sec, 6.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12643, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 145, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (172.46, 103.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (240.52, 124.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.930 CPU sec, 6.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12746, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 95, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (140.73, 116.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.23, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.804 CPU sec, 7.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12608, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 117, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (162.14, 138.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.14, 125.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.845 CPU sec, 6.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12693, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 120, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (113.43, 67.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (239.91, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.161 CPU sec, 6.716 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12632, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 124, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 235) [M::mem_pestat] mean and std.dev: (120.62, 36.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (237.79, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.468 CPU sec, 8.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12737, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 168, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (174.28, 106.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.82, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.355 CPU sec, 6.840 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12665, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (135.38, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.04, 122.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.747 CPU sec, 6.352 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12590, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 160, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (172.54, 97.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (236.56, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.402 CPU sec, 6.091 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12689, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (163.00, 113.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.38, 120.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.856 CPU sec, 6.233 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12764, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 107, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (136.47, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.51, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.027 CPU sec, 6.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12634, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 161, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (168.50, 91.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.82, 121.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.924 CPU sec, 6.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12662, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 110, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (139.28, 78.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.53, 119.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.231 CPU sec, 6.522 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12426, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 153, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (194.47, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.07, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 197.289 CPU sec, 7.788 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12607, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 195, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (199.67, 95.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.55, 120.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 187.826 CPU sec, 7.378 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12660, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 140, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (199.53, 133.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.72, 122.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.410 CPU sec, 6.537 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12535, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 124, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 230) [M::mem_pestat] mean and std.dev: (117.82, 31.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (240.17, 124.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 179.116 CPU sec, 6.518 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12474, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 113, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (124.65, 78.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (239.41, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.283 CPU sec, 6.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12534, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 151, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (153.36, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.80, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.490 CPU sec, 7.039 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12680, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 137, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (136.50, 50.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.18, 123.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.606 CPU sec, 6.671 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12523, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 135, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (153.86, 90.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.98, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.165 CPU sec, 6.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12592, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 137, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (176.88, 101.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.00, 123.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.189 CPU sec, 6.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12807, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 147, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (178.47, 106.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.96, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.252 CPU sec, 5.896 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12549, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 145, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (167.35, 109.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (239.73, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.578 CPU sec, 7.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12585, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 135, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (173.57, 113.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (239.01, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.977 CPU sec, 6.603 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12661, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 119, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (165.17, 112.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (238.72, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.215 CPU sec, 7.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12699, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 129, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (142.08, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.03, 124.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.995 CPU sec, 6.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12695, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 212, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (192.53, 87.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.65, 124.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.587 CPU sec, 7.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12677, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 112, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (105.37, 63.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (241.66, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 193.257 CPU sec, 7.008 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12485, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 117, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (209.86, 184.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (232.88, 119.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.231 CPU sec, 5.933 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 12293, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (142.35, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (229.91, 117.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 186.772 CPU sec, 7.108 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12451, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (183.87, 104.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.29, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.319 CPU sec, 6.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 12527, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 161, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (177.09, 73.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (231.99, 117.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.083 CPU sec, 7.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12316, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 168, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (183.90, 96.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (229.02, 116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.979 CPU sec, 5.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12349, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 141, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (181.00, 140.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (228.88, 115.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.845 CPU sec, 6.895 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12331, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 225, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (213.12, 122.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.44, 115.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.402 CPU sec, 6.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12116, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 150, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (178.84, 101.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (225.28, 114.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.590 CPU sec, 6.296 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 12186, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 152, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (182.69, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (224.05, 112.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 185.484 CPU sec, 6.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12090, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 109, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (144.86, 95.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (222.37, 111.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 188.251 CPU sec, 6.673 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 11994, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 157, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (174.14, 85.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (225.55, 112.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 181.333 CPU sec, 7.726 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 11916, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 156, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (153.00, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.61, 112.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.644 CPU sec, 8.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 11865, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 140, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (202.56, 169.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (227.96, 115.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.577 CPU sec, 6.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12613, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 159, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (181.15, 116.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (236.63, 122.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.736 CPU sec, 6.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12624, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 161, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (178.08, 83.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.57, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.984 CPU sec, 6.103 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 12514, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 175, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (184.81, 112.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.47, 120.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 171.747 CPU sec, 6.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12637, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (161.95, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.88, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 172.984 CPU sec, 6.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12680, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 146, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (181.61, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (239.37, 124.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.266 CPU sec, 7.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 12720, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 151, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (191.94, 149.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.29, 121.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.681 CPU sec, 6.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12775, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 106, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (113.89, 67.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.19, 121.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 173.474 CPU sec, 6.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12630, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 104, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (102.90, 49.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.53, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 192.186 CPU sec, 7.471 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12734, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 164, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (179.60, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (235.84, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 178.451 CPU sec, 6.433 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 12747, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 169, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (186.86, 113.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (232.56, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 194.070 CPU sec, 7.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12657, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 166, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (161.88, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (236.21, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.793 CPU sec, 7.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12831, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 153, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (158.88, 100.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (232.85, 119.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 174.569 CPU sec, 6.182 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 12626, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (140.65, 87.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.84, 121.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.510 CPU sec, 6.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12550, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 173, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (187.17, 97.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.00, 120.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 175.913 CPU sec, 6.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12657, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 196, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (188.22, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (239.13, 122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.466 CPU sec, 6.081 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12639, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (201.64, 101.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (238.44, 121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 165.262 CPU sec, 6.048 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 12539, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 183, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (179.43, 87.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (236.58, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 169.924 CPU sec, 5.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12507, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 108, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (113.18, 58.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (234.55, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.429 CPU sec, 6.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 12687, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 162, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (175.32, 91.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (238.37, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 182.242 CPU sec, 6.387 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 12573, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (201.68, 174.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (238.70, 123.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 184.379 CPU sec, 7.962 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12751, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 210, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (204.87, 103.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.92, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.236 CPU sec, 6.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12766, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 200, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (191.69, 108.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.31, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 180.408 CPU sec, 7.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12760, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 232, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (211.33, 109.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (238.79, 123.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 176.183 CPU sec, 6.322 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 12541, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 141, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (157.53, 103.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.97, 122.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 168.690 CPU sec, 6.031 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12660, 3, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 109, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (149.86, 94.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.89, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 167.985 CPU sec, 6.355 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12548, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 131, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (170.05, 105.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (240.31, 124.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 177.487 CPU sec, 7.367 real sec [M::process] read 35658 sequences (5384358 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 12747, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 145, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (176.95, 115.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.48, 122.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 183.871 CPU sec, 6.385 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 6812, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 205, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (239.14, 123.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 35658 reads in 90.281 CPU sec, 3.521 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21190\tSM:UG10S2P661\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21190_HLKNYBGXX_UG10S2P661_R1.fastq.gz 5347_7332_21190_HLKNYBGXX_UG10S2P661_R2.fastq.gz [main] Real time: 7646.603 sec; CPU: 205166.218 sec Finished alignement of UG10S2P661 at Thu Apr 4 00:13:28 2019 Sorting BAM file started at Thu Apr 4 00:13:28 2019 samtools sort -@ 30 -m 4G UG10S2P661_unsorted.bam -o UG10S2P661.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Thu Apr 4 00:13:28 2019 rm UG10S2P661_unsorted.bam rm 5347_7332_21190_HLKNYBGXX_UG10S2P661_R1.fastq.gz rm 5347_7332_21190_HLKNYBGXX_UG10S2P661_R2.fastq.gz samtools index UG10S2P661.bam Finished BAM file generation of the sample - UG10S2P661 Processing fastq files for the sample - UG10S2P652 Reading 5347_7332_21191_HLKNYBGXX_UG10S2P652_R1.fastq.gz 5347_7332_21191_HLKNYBGXX_UG10S2P652_R2.fastq.gz bwa mem -t 30 -R '@RG\tID:21191\tSM:UG10S2P652\tLB:lib1\tPL:ILLUMINA' -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21191_HLKNYBGXX_UG10S2P652_R1.fastq.gz 5347_7332_21191_HLKNYBGXX_UG10S2P652_R2.fastq.gz | samtools view -Sb1 - > UG10S2P652_unsorted.bam [M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 66226 sequences (10000126 bp)... [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13227, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 148, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (169.88, 93.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (237.46, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.035 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13409, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 92, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (154.15, 135.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.02, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.039 CPU sec, 4.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13472, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.57, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.250 CPU sec, 5.007 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13652, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 186, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (212.22, 127.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.67, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.677 CPU sec, 5.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13698, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 205, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (207.79, 121.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.59, 123.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.526 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13664, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 119, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (158.79, 110.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.24, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.872 CPU sec, 5.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13694, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 160, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (128.80, 57.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.61, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.806 CPU sec, 4.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13645, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 178, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (195.85, 142.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.63, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.845 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13767, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 148, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 288) [M::mem_pestat] mean and std.dev: (134.91, 45.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (237.99, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.528 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13811, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 122, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (133.46, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.55, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.667 CPU sec, 4.700 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13870, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 196, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (189.12, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.49, 126.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.933 CPU sec, 5.094 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13758, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 229, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (217.23, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (241.83, 125.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.609 CPU sec, 4.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13652, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 215, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (192.57, 102.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.68, 126.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.620 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13542, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 138, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (146.27, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.45, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.200 CPU sec, 5.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13627, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 171, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (181.73, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.69, 124.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.769 CPU sec, 4.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13724, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 125, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (178.00, 108.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (238.99, 123.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.055 CPU sec, 4.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13730, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 178, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (163.53, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (240.31, 126.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.052 CPU sec, 4.396 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13779, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 140, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (162.85, 68.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.99, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.572 CPU sec, 4.692 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13490, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 157, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (168.58, 103.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.05, 125.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.668 CPU sec, 4.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13626, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 195, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (178.27, 95.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (236.63, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.113 CPU sec, 4.389 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13751, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (238.12, 124.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.442 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13546, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.86, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.526 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13541, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 184, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (178.61, 138.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.14, 122.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.225 CPU sec, 5.414 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13816, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 95, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (86.11, 30.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.84, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.882 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13847, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 123, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (205.32, 154.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (234.18, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.262 CPU sec, 4.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13631, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 150, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (152.06, 60.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.63, 120.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.123 CPU sec, 4.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13825, 7, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.22, 122.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 108.919 CPU sec, 3.922 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13662, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 127, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (158.44, 111.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.40, 121.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.888 CPU sec, 4.969 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13768, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 158, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (166.07, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.22, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.975 CPU sec, 4.447 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13801, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (151.27, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.50, 120.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.628 CPU sec, 4.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13778, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 176, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 263) [M::mem_pestat] mean and std.dev: (174.33, 26.70) [M::mem_pestat] low and high boundaries for proper pairs: (68, 295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.49, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.323 CPU sec, 4.861 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13507, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 172, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (248.38, 207.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (234.11, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.580 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13600, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 219, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (194.44, 110.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.35, 119.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.475 CPU sec, 4.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13570, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 179, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (194.42, 105.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (230.67, 119.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.836 CPU sec, 4.879 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13394, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (118.77, 67.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.00, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.918 CPU sec, 5.078 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13244, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 192, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (153.83, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (236.24, 122.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.318 CPU sec, 5.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13419, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (209.17, 106.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.86, 123.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.610 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13312, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 199, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (234.07, 88.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.95, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.584 CPU sec, 4.793 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13321, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.25, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.999 CPU sec, 5.475 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13586, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 219, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.00, 150.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.14, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.074 CPU sec, 5.490 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13574, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 152, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (145.14, 71.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.98, 123.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.585 CPU sec, 4.742 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13691, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 146, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (163.94, 84.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.26, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.088 CPU sec, 4.343 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13709, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (125.42, 50.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (239.24, 124.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.593 CPU sec, 4.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13654, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 233, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (161.10, 87.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.29, 123.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.472 CPU sec, 4.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13864, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 131, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (125.56, 60.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.28, 123.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.390 CPU sec, 5.468 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13714, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 175, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (169.27, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.61, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.964 CPU sec, 5.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13798, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 143, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (161.70, 107.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.76, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.889 CPU sec, 4.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13673, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 126, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (139.53, 90.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.96, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.447 CPU sec, 4.437 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13642, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 173, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (179.27, 104.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.61, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.613 CPU sec, 5.017 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13761, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 140, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (146.68, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.09, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.736 CPU sec, 4.185 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13729, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 202, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (216.73, 170.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.25, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.374 CPU sec, 4.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13852, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.37, 123.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.310 CPU sec, 4.181 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13639, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 136, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (167.60, 102.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.77, 124.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.687 CPU sec, 4.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13438, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 176, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (178.20, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.71, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.214 CPU sec, 5.196 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13763, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 123, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (185.35, 124.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (237.26, 124.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.866 CPU sec, 4.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13641, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 138, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (149.55, 73.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.26, 123.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.868 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13822, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 131, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (166.25, 121.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.77, 122.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.379 CPU sec, 4.390 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13703, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 142, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (152.72, 100.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (235.72, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.980 CPU sec, 4.360 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13628, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 90, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (147.64, 124.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (233.35, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.883 CPU sec, 4.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13906, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 112, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (135.56, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (233.16, 121.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.099 CPU sec, 4.303 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13604, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 216, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (199.85, 105.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (232.20, 120.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.233 CPU sec, 4.869 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13772, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 177, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (164.79, 48.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.66, 121.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.477 CPU sec, 5.127 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13905, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 221, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (187.00, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (231.20, 120.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.150 CPU sec, 4.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13709, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 111, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (118.65, 49.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.93, 121.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.893 CPU sec, 5.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13710, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 106, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (150.00, 99.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (230.46, 118.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.622 CPU sec, 4.614 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13598, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 249, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (253.67, 96.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.09, 118.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.603 CPU sec, 4.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13699, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 242, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (214.15, 133.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.54, 120.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.342 CPU sec, 4.966 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13612, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 138, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (171.31, 106.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.72, 120.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.021 CPU sec, 4.273 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13635, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.45, 118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.266 CPU sec, 5.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13460, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.85, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 132.807 CPU sec, 4.884 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13393, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 228, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (226.08, 137.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.35, 123.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.625 CPU sec, 4.287 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13374, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 163, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (200.50, 108.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (235.38, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.516 CPU sec, 4.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13314, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 173, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (162.73, 75.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.09, 122.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.790 CPU sec, 4.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13656, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.77, 121.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.317 CPU sec, 4.113 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13602, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 186, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (255.00, 140.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.77, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.917 CPU sec, 5.075 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13595, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 125, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (162.50, 123.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.43, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.775 CPU sec, 5.620 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13559, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 145, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (202.54, 136.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.67, 122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.174 CPU sec, 4.315 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13627, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 115, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (152.62, 81.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.94, 122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.083 CPU sec, 4.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13561, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 144, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (147.42, 61.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.91, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.083 CPU sec, 4.449 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13567, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 182, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (202.85, 116.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.91, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.720 CPU sec, 4.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13574, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 105, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (142.80, 78.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (237.61, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.121 CPU sec, 4.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13578, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 152, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (198.81, 120.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (238.58, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.489 CPU sec, 4.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13667, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 240, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (197.38, 117.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (238.71, 125.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.128 CPU sec, 4.140 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13605, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (200.64, 94.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.80, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.244 CPU sec, 5.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13690, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 151, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (173.71, 84.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.21, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.405 CPU sec, 4.839 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13544, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 150, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (194.32, 114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.18, 124.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.469 CPU sec, 4.403 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13484, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 284, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (258.78, 178.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.93, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.594 CPU sec, 4.979 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13537, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 225, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (214.50, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.42, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.513 CPU sec, 5.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13604, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 156, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (134.90, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.36, 122.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.317 CPU sec, 4.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13610, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 140, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (117.23, 69.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.63, 121.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.526 CPU sec, 4.694 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13580, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.86, 122.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.403 CPU sec, 5.193 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13564, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 151, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (122.47, 54.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.38, 121.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.806 CPU sec, 4.903 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13505, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 186, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (220.00, 146.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.52, 122.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.963 CPU sec, 5.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13627, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 167, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (177.27, 112.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (233.32, 121.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.340 CPU sec, 4.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13752, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 113, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (180.94, 151.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.46, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.931 CPU sec, 4.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13588, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 236, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (231.17, 113.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.67, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.071 CPU sec, 5.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13755, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 178, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (182.83, 94.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.90, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.616 CPU sec, 4.175 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13737, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (231.37, 120.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.442 CPU sec, 4.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13633, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 160, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (134.11, 47.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.50, 119.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.709 CPU sec, 4.158 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13507, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 181, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (199.83, 108.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.55, 119.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.868 CPU sec, 4.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13602, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 145, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (147.33, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.48, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.628 CPU sec, 4.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13622, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 222, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (219.00, 109.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.60, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.449 CPU sec, 5.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13642, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 214, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (189.94, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.88, 120.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.226 CPU sec, 5.268 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13438, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 107, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (128.45, 58.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.44, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.054 CPU sec, 4.843 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13180, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 108, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (108.55, 59.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.99, 117.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.293 CPU sec, 4.615 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13360, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 167, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (194.50, 103.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (229.40, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.201 CPU sec, 4.498 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13579, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 131, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (142.36, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (231.80, 119.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.924 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13650, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 171, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (137.93, 70.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.99, 120.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.018 CPU sec, 4.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13569, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 174, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (195.27, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.61, 120.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.049 CPU sec, 4.714 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13638, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.37, 122.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.459 CPU sec, 4.305 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13742, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 229, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (220.16, 129.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (235.02, 123.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.066 CPU sec, 5.154 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13723, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 169, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (173.85, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.02, 123.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.824 CPU sec, 4.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13685, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 214, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (208.60, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (236.42, 123.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.785 CPU sec, 4.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13686, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 148, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (140.93, 93.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.21, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.928 CPU sec, 4.812 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13622, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.21, 124.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.761 CPU sec, 5.028 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13588, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 220, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (283.36, 198.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (236.84, 124.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.747 CPU sec, 5.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13408, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 186, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (175.50, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.52, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.736 CPU sec, 4.473 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13696, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 189, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (181.31, 106.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.84, 122.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.027 CPU sec, 5.087 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13754, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 157, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (152.92, 88.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.79, 122.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.309 CPU sec, 4.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13714, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 179, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (155.62, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (234.88, 123.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.211 CPU sec, 3.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13543, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (235.47, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.152 CPU sec, 4.344 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13758, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 164, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (179.85, 118.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.14, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.220 CPU sec, 4.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13694, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 179, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (185.19, 133.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.13, 121.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.677 CPU sec, 4.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13622, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (194.11, 115.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.86, 122.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.841 CPU sec, 5.709 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13635, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (176.43, 116.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.60, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.750 CPU sec, 4.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13725, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 132, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538) [M::mem_pestat] mean and std.dev: (142.21, 84.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.41, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.391 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13643, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 131, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (153.87, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (232.85, 120.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.002 CPU sec, 4.777 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13801, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (143.55, 64.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.52, 119.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.222 CPU sec, 5.191 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13717, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 145, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (167.93, 105.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (230.09, 117.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.769 CPU sec, 4.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13659, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 193, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (216.08, 129.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (231.58, 120.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.482 CPU sec, 4.425 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13688, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.33, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.052 CPU sec, 4.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13683, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 124, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (137.38, 84.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.52, 118.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.755 CPU sec, 4.971 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13473, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.27, 119.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 114.054 CPU sec, 4.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13620, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 185, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (179.44, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (229.37, 117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.296 CPU sec, 4.353 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13530, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 155, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (181.17, 114.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (228.65, 116.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.081 CPU sec, 4.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13632, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 147, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (155.89, 81.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (227.03, 117.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.681 CPU sec, 4.053 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13618, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (230.37, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 112.677 CPU sec, 4.555 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13506, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 144, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (188.71, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (229.40, 118.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.741 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 13162, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (226.84, 116.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.292 CPU sec, 4.354 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13431, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 229, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (187.40, 102.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.84, 116.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.829 CPU sec, 5.829 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13303, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 140, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.50, 63.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (229.81, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.637 CPU sec, 5.009 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13398, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 142, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (145.30, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.61, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.690 CPU sec, 4.681 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13491, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 160, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (176.56, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.36, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 136.562 CPU sec, 5.195 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13589, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 181, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (173.75, 88.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.73, 120.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.950 CPU sec, 5.370 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13676, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (233.27, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 125.748 CPU sec, 4.787 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13772, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.79, 122.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.702 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13752, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 192, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (169.18, 95.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (234.87, 123.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.500 CPU sec, 5.019 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13894, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 132, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (196.00, 141.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (234.42, 121.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.780 CPU sec, 4.944 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13584, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (95.73, 64.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.22, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.219 CPU sec, 5.507 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13738, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 207, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (197.14, 94.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.07, 122.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.313 CPU sec, 5.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13688, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.83, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.020 CPU sec, 4.900 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13701, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (39, 91, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (105.36, 88.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.34, 122.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.667 CPU sec, 4.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13577, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 135, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (134.73, 64.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.58, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.158 CPU sec, 5.161 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13652, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 153, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (241.45, 185.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.40, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.586 CPU sec, 4.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13654, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 165, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (200.29, 118.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.81, 124.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.035 CPU sec, 4.566 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13791, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 124, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (138.41, 65.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.00, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.933 CPU sec, 4.628 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13505, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 154, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (134.22, 49.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.13, 123.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.810 CPU sec, 6.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13622, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 192, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (185.79, 114.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.60, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.615 CPU sec, 4.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13631, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 113, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (127.50, 69.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.65, 120.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.054 CPU sec, 4.891 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13468, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 142, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (190.73, 111.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.56, 122.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.515 CPU sec, 5.458 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13610, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (31, 98, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (84.55, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (232.97, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.555 CPU sec, 5.667 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13527, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.05, 120.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 113.653 CPU sec, 3.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13638, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 196, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (183.83, 115.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.00, 119.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.272 CPU sec, 4.192 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13676, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (144.20, 87.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.01, 117.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.688 CPU sec, 4.064 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13557, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.86, 118.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.826 CPU sec, 5.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13610, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 164, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (154.38, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (228.79, 117.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.487 CPU sec, 4.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13760, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 181, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (191.78, 116.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (227.84, 116.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.869 CPU sec, 3.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13721, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 115, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (118.27, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (230.94, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.676 CPU sec, 4.379 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13737, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 185, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (160.85, 54.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (229.14, 117.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.454 CPU sec, 4.092 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13590, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 153, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (175.79, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (229.27, 118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.785 CPU sec, 4.163 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13639, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 139, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (145.33, 83.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (229.87, 119.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.996 CPU sec, 4.474 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13531, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 119, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (158.79, 90.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.30, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.922 CPU sec, 4.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13350, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 117, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (146.50, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.01, 116.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.279 CPU sec, 4.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13189, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 164, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (149.50, 81.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.57, 117.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.790 CPU sec, 4.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13298, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 129, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (116.00, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (227.65, 117.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.342 CPU sec, 5.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13428, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 98, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (96.38, 30.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.83, 116.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.701 CPU sec, 4.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13449, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 246, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (202.15, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (231.23, 119.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.401 CPU sec, 4.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13442, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 181, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (171.42, 132.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (229.28, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.917 CPU sec, 4.345 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13677, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 122, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (142.15, 70.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (230.97, 120.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.431 CPU sec, 4.911 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13479, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (146.77, 81.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (231.68, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.714 CPU sec, 4.767 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13714, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 218, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (210.38, 116.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.55, 121.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.356 CPU sec, 5.047 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13451, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 199, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (193.94, 108.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.33, 121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.940 CPU sec, 5.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13583, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 163, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (195.62, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.70, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.727 CPU sec, 4.968 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13713, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 168, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (170.40, 97.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (234.81, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.381 CPU sec, 4.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13489, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 94, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (116.57, 52.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.29, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.998 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 13550, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 159, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (173.08, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.04, 122.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.358 CPU sec, 4.644 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13626, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 184, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (185.00, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.51, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.338 CPU sec, 4.725 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13609, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 172, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (144.82, 67.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (233.98, 121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.886 CPU sec, 4.912 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13643, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 169, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (170.10, 75.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.54, 120.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.688 CPU sec, 5.245 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13676, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 171, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (154.64, 72.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.14, 120.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.473 CPU sec, 4.730 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13534, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 197, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (183.47, 88.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.20, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.214 CPU sec, 5.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13622, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 160, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (205.75, 134.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.31, 119.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.594 CPU sec, 4.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13520, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 200, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (177.93, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.72, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.796 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13680, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 93, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (125.10, 79.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.65, 119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.586 CPU sec, 4.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13683, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 120, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (137.67, 86.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.75, 119.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.511 CPU sec, 4.290 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13555, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 149, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (158.38, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (233.23, 120.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.517 CPU sec, 4.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 13714, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.90, 118.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.416 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13531, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 146, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (177.41, 130.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (229.46, 118.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.794 CPU sec, 4.323 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13484, 10, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 144, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (135.21, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.81, 118.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (139, 353, 2704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7834) [M::mem_pestat] mean and std.dev: (1521.60, 2201.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10399) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 66226 reads in 113.766 CPU sec, 4.388 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13639, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (228.53, 117.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.695 CPU sec, 5.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13535, 6, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (227.76, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 114.198 CPU sec, 5.242 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13494, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 218, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 447) [M::mem_pestat] mean and std.dev: (213.38, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.69, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.131 CPU sec, 3.898 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13493, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (108.92, 59.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.97, 115.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.328 CPU sec, 5.126 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13550, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 138, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (136.45, 55.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.76, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.190 CPU sec, 4.905 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13571, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (228.94, 117.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.109 CPU sec, 4.881 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13379, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 141, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (160.00, 95.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (228.93, 117.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.983 CPU sec, 4.532 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13481, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 141, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (200.00, 147.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.00, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.136 CPU sec, 4.095 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13716, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 167, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (184.94, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.27, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.987 CPU sec, 3.886 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13772, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 139, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (117.83, 42.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.19, 122.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.769 CPU sec, 4.398 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13747, 2, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 126, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (128.81, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.43, 126.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.482 CPU sec, 5.111 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13737, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 119, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (141.36, 71.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.72, 124.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.058 CPU sec, 4.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13734, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 229, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (205.71, 101.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.95, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.580 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13594, 4, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.85, 124.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.924 CPU sec, 5.336 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13797, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 175, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (176.33, 99.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.67, 123.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.719 CPU sec, 4.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13707, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 108, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (132.09, 76.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.21, 124.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.835 CPU sec, 4.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13824, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 167, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (187.47, 108.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (237.04, 124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.745 CPU sec, 5.621 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13625, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 168, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (192.89, 129.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 866) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (238.19, 123.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.997 CPU sec, 5.150 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13634, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.66, 124.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.796 CPU sec, 4.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13862, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 168, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (188.24, 82.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.28, 124.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.486 CPU sec, 4.222 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13537, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 235, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (218.25, 90.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (236.03, 123.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.323 CPU sec, 4.545 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13597, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 169, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (176.92, 55.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (238.36, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.479 CPU sec, 4.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13662, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 104, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (168.08, 161.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.50, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.559 CPU sec, 5.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13718, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.63, 123.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.018 CPU sec, 4.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13574, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 106, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (114.17, 84.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.80, 123.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.372 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13426, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 209, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (187.88, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.52, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.402 CPU sec, 4.904 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13698, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 187, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (165.00, 96.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.61, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 103.823 CPU sec, 3.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13755, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 96, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (102.07, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.79, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.523 CPU sec, 4.120 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13580, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 155, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (121.00, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (235.88, 122.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.329 CPU sec, 4.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13908, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (234.20, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.128 CPU sec, 4.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13624, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 134, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (153.36, 53.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (237.63, 123.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.117 CPU sec, 4.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13503, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 143, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (175.56, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (234.16, 121.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.042 CPU sec, 4.264 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13582, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 182, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (217.19, 145.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.81, 119.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 104.259 CPU sec, 3.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13708, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 123, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (127.95, 60.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (230.03, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.452 CPU sec, 4.056 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13494, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 161, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (165.69, 72.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (230.67, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.274 CPU sec, 3.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13420, 5, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.32, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 101.769 CPU sec, 3.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13449, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 140, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (133.50, 78.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (227.85, 117.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 101.436 CPU sec, 4.184 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13543, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 177, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (182.38, 86.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (227.60, 116.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.340 CPU sec, 3.956 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13220, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.13, 116.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 103.962 CPU sec, 4.813 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13159, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 139, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (159.15, 78.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (224.26, 113.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.224 CPU sec, 4.492 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13076, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 187, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (199.44, 96.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (222.85, 112.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.485 CPU sec, 4.836 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13014, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 271, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (225.69, 106.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (225.41, 116.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.894 CPU sec, 4.913 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13336, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 136, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (183.45, 113.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.77, 123.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.488 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13594, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 208, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (172.18, 90.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (242.55, 126.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.501 CPU sec, 4.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13603, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 122, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (131.00, 92.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (240.85, 125.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.702 CPU sec, 5.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13637, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 197, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (193.93, 126.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.40, 126.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.816 CPU sec, 5.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13718, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 178, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (197.28, 98.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (242.12, 126.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.994 CPU sec, 4.522 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13783, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 158, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (170.78, 114.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.24, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.765 CPU sec, 4.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13696, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 112, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (142.81, 92.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 206, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (240.78, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.710 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13727, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 176, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (187.91, 87.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.29, 126.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.019 CPU sec, 4.937 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13658, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 163, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (164.00, 79.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (241.87, 125.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.730 CPU sec, 4.557 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13649, 8, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (241.46, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.437 CPU sec, 5.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13706, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 116, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (139.67, 90.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (241.11, 126.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.290 CPU sec, 4.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13754, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 140, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (138.58, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.18, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.749 CPU sec, 5.583 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13721, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 181, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (174.75, 87.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.47, 125.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.039 CPU sec, 5.547 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13541, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.68, 126.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.196 CPU sec, 4.878 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13686, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 124, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (107.20, 36.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.69, 126.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.593 CPU sec, 5.324 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13651, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (242.88, 127.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.625 CPU sec, 4.824 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13691, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 215, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (176.15, 91.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.21, 123.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.367 CPU sec, 5.292 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13754, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.93, 125.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.685 CPU sec, 4.277 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13637, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 171, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (141.17, 62.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.51, 124.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.172 CPU sec, 4.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13735, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (240.95, 126.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 113.488 CPU sec, 4.271 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13736, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 137, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (163.31, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (237.64, 125.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.344 CPU sec, 4.229 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13724, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 142, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (170.69, 100.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.90, 123.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.413 CPU sec, 5.859 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13598, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 147, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (202.50, 162.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (238.00, 125.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.305 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13561, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.29, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.935 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13688, 8, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.64, 121.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.613 CPU sec, 5.463 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13667, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 245, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (281.70, 145.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (232.95, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.989 CPU sec, 5.755 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13504, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 127, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (156.38, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (230.38, 119.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.977 CPU sec, 4.417 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13435, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 154, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (153.71, 89.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (228.11, 117.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.917 CPU sec, 4.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13373, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 79, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (101.73, 60.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (227.78, 116.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.163 CPU sec, 4.478 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13359, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 175, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (194.65, 92.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (228.05, 117.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.357 CPU sec, 4.719 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13426, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 158, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (159.13, 86.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (224.98, 114.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.826 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13237, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 84, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (153.47, 122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (226.87, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.122 CPU sec, 4.871 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12988, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 179, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (175.85, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (222.66, 112.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.571 CPU sec, 5.594 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12891, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 165, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (158.20, 89.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (225.17, 114.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.392 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12748, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 79, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (105.47, 62.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (228.99, 118.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.742 CPU sec, 5.238 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13459, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 240, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (226.87, 118.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 206, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.80, 127.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.824 CPU sec, 4.338 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13569, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (244.81, 129.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.347 CPU sec, 4.411 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13559, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (151.70, 92.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (239.82, 126.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.142 CPU sec, 5.544 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13571, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (156.94, 117.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 207, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (241.36, 125.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.210 CPU sec, 4.391 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13752, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 144, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (125.00, 47.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (241.06, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.443 CPU sec, 5.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13653, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (240.71, 126.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.967 CPU sec, 4.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13506, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 125, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (169.15, 91.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.17, 125.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.519 CPU sec, 4.663 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13699, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 143, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (140.77, 70.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (239.02, 125.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.288 CPU sec, 4.598 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13631, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 202, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (178.41, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (240.67, 126.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.857 CPU sec, 5.155 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13649, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.53, 124.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 112.832 CPU sec, 4.690 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13818, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 126, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (144.36, 105.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (239.15, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.928 CPU sec, 4.331 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13704, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.78, 125.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.355 CPU sec, 5.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13706, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 228, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (190.00, 103.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.20, 124.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.651 CPU sec, 4.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13598, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 227, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (227.62, 130.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (240.17, 126.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.909 CPU sec, 5.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13617, 9, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 244, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (199.28, 122.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (235.69, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.390 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13568, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (224.42, 107.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.30, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.957 CPU sec, 4.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13579, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 105, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (94.00, 52.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.59, 123.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.917 CPU sec, 5.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13571, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 153, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (152.89, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.87, 125.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.984 CPU sec, 4.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13782, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 158, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (160.17, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (237.63, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.034 CPU sec, 4.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13652, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 128, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (152.75, 114.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (236.70, 125.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.769 CPU sec, 4.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13545, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 205, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (219.46, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.50, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.890 CPU sec, 4.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13619, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 127, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (183.56, 100.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.03, 123.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.472 CPU sec, 4.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13574, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 138, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (138.69, 68.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.79, 122.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.404 CPU sec, 4.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13509, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (231.53, 120.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 111.886 CPU sec, 4.561 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13554, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 144, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (153.82, 89.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.25, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.214 CPU sec, 4.135 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13624, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 181, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (184.67, 94.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.49, 116.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.995 CPU sec, 5.476 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13540, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 151, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (161.13, 100.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.34, 118.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.769 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13470, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 181, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.15, 69.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (226.55, 115.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.288 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13364, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 154, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (156.00, 104.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (226.57, 114.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.050 CPU sec, 4.275 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13368, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 221, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (238.54, 99.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (222.65, 114.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.050 CPU sec, 4.973 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13133, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 207, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (200.33, 99.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (224.95, 114.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.609 CPU sec, 4.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13170, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 161, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (177.47, 103.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (223.46, 113.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.828 CPU sec, 6.042 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13071, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 224, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (207.08, 96.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (223.23, 113.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.042 CPU sec, 5.090 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 12726, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 151, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (180.60, 116.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (228.56, 117.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.235 CPU sec, 4.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13289, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 169, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (172.41, 94.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.43, 122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.720 CPU sec, 5.809 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13656, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 134, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (161.68, 96.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.13, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.081 CPU sec, 4.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13567, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 228, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (250.00, 106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (234.69, 122.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.811 CPU sec, 5.100 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13565, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 128, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (112.44, 32.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.27, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.545 CPU sec, 5.214 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13632, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 111, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (100.64, 67.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.56, 121.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.778 CPU sec, 5.480 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13569, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 133, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (148.14, 78.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.76, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.769 CPU sec, 3.820 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13625, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 116, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (138.36, 70.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (236.87, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.678 CPU sec, 4.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13644, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (147.83, 115.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.59, 122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.931 CPU sec, 4.794 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13686, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 180, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (195.60, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.42, 122.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.230 CPU sec, 5.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13729, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 164, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (160.77, 83.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.31, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.542 CPU sec, 4.965 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13639, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (142.94, 60.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (236.14, 123.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.720 CPU sec, 4.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13733, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.35, 121.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.725 CPU sec, 5.194 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13406, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 141, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (167.00, 134.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.41, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.077 CPU sec, 5.052 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13629, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 214, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (173.90, 111.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.40, 122.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.156 CPU sec, 4.657 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13688, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 98, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (109.36, 56.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.79, 123.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.975 CPU sec, 4.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13840, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 96, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (152.23, 87.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.07, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.017 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13666, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 182, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (191.36, 101.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.83, 122.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.355 CPU sec, 4.485 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13624, 4, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.57, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.736 CPU sec, 4.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 13636, 4, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.25, 122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.545 CPU sec, 5.494 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13661, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 172, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (172.71, 99.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.79, 122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.427 CPU sec, 4.920 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13694, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 146, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (123.64, 56.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (233.27, 119.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.876 CPU sec, 4.419 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13509, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 188, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (187.63, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.98, 121.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.891 CPU sec, 4.306 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13739, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 183, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (229.39, 165.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.88, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.964 CPU sec, 4.093 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13587, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (126.91, 77.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.24, 119.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.599 CPU sec, 3.748 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13429, 6, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 165, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (178.35, 95.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (228.57, 118.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.071 CPU sec, 4.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13752, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 174, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (332.10, 352.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (227.77, 116.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.285 CPU sec, 4.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13632, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (35, 100, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (148.80, 137.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.31, 116.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.474 CPU sec, 4.307 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13604, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 99, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (131.64, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (224.12, 114.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.510 CPU sec, 3.791 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13549, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 127, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (158.73, 92.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (224.63, 114.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.376 CPU sec, 4.420 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13461, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 129, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (172.50, 83.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (225.05, 114.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.435 CPU sec, 4.531 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13471, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 165, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (222.50, 154.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (224.19, 113.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.238 CPU sec, 4.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13196, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (219.36, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (221.63, 111.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.964 CPU sec, 4.750 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13027, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 231, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (207.75, 92.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (222.23, 112.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.235 CPU sec, 4.500 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12926, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 147, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (148.27, 98.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (219.42, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.701 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 12955, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 252, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (276.89, 219.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (226.86, 115.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.980 CPU sec, 4.302 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13641, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 237, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (230.00, 111.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (235.98, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.971 CPU sec, 4.998 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13738, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 234, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (192.80, 97.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.56, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.004 CPU sec, 5.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13591, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (239.38, 125.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.019 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13492, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 153, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (188.73, 111.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.44, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.036 CPU sec, 4.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13733, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 101, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (162.47, 124.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (238.64, 124.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.493 CPU sec, 4.330 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13753, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 120, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (138.44, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.52, 122.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.248 CPU sec, 4.444 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13555, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 204, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (197.60, 83.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.96, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.608 CPU sec, 6.024 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13661, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 143, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (106.64, 60.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.89, 124.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.924 CPU sec, 4.416 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13826, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 127, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (179.11, 126.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (237.88, 123.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.396 CPU sec, 4.542 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13622, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 177, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (181.36, 110.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.78, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.671 CPU sec, 4.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13621, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 157, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (257.58, 253.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.35, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.205 CPU sec, 4.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13581, 0, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 129, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (152.05, 88.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.26, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.867 CPU sec, 4.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13526, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 91, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (110.45, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.15, 123.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.180 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13756, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 190, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (198.75, 102.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.23, 123.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.928 CPU sec, 4.446 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13707, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 91, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (123.40, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (236.44, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.220 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13673, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 267, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (207.00, 107.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.74, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.900 CPU sec, 4.925 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13819, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.90, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.562 CPU sec, 5.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13639, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 163, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (138.45, 88.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.06, 122.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.503 CPU sec, 4.988 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13639, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 206, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (189.14, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (235.11, 123.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.161 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13675, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 167, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (163.28, 84.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (234.14, 122.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.794 CPU sec, 4.946 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13722, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 125, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (166.58, 116.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (232.80, 121.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.229 CPU sec, 3.846 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13660, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 183, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (166.50, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.24, 121.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.553 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13703, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 209, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (206.00, 125.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.73, 122.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.884 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13593, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 121, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (137.59, 74.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (230.58, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.276 CPU sec, 3.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13737, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 156, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (156.73, 65.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (227.07, 115.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.497 CPU sec, 3.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13651, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 144, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (220.33, 143.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (229.01, 117.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.973 CPU sec, 4.693 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13579, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 116, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (110.85, 58.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.56, 114.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 107.241 CPU sec, 3.833 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13510, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (128.15, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (225.06, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.706 CPU sec, 5.243 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13395, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 180, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (177.63, 96.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (223.48, 113.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.385 CPU sec, 5.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13355, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 183, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (174.81, 103.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (224.14, 113.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.455 CPU sec, 4.992 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13396, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (135.07, 78.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (222.02, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.352 CPU sec, 4.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13088, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 192, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (228.80, 153.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (218.49, 109.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.240 CPU sec, 4.602 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12860, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 176, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (213.36, 126.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (218.84, 109.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.222 CPU sec, 5.325 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 12933, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 168, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (167.37, 112.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (224.64, 113.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.933 CPU sec, 5.810 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13495, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 105, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (111.73, 64.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 206, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (241.28, 126.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.731 CPU sec, 4.457 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13515, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 128, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (134.71, 76.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.92, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.267 CPU sec, 4.363 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13526, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 198, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (197.86, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.64, 123.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.728 CPU sec, 6.311 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13637, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 137, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (138.86, 65.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.35, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.633 CPU sec, 4.713 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13699, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 184, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (212.74, 127.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.86, 123.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.167 CPU sec, 4.927 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13586, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 116, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (162.09, 83.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.76, 124.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.041 CPU sec, 4.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13584, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 139, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (176.64, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.62, 122.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.571 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13670, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.00, 123.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.148 CPU sec, 4.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13798, 7, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 205, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (224.92, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (237.19, 122.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.718 CPU sec, 4.578 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13534, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 205, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (195.93, 106.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.68, 124.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.865 CPU sec, 4.948 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13625, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 136, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (207.23, 177.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.63, 123.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.228 CPU sec, 4.382 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13490, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 219, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (204.55, 160.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.20, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.146 CPU sec, 5.002 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13656, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 187, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (150.27, 86.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.12, 121.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.887 CPU sec, 5.496 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13675, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 175, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (170.90, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.43, 124.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.133 CPU sec, 6.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13627, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 212, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (204.82, 108.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.48, 124.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.152 CPU sec, 4.517 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13648, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 128, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (175.62, 93.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.48, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.310 CPU sec, 6.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13561, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 110, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (156.86, 134.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.94, 122.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.831 CPU sec, 4.704 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13621, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.53, 122.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 133.066 CPU sec, 4.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13599, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 174, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (190.11, 125.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.08, 120.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.587 CPU sec, 5.174 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13526, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 190, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (210.83, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.78, 120.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.071 CPU sec, 4.924 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13714, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 132, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (132.45, 62.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.98, 119.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.918 CPU sec, 5.069 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13615, 5, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.64, 120.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 114.850 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13591, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 132, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (153.46, 92.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.64, 118.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.907 CPU sec, 4.442 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13636, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 152, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (182.54, 116.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.70, 118.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.789 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13343, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 108, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (162.69, 163.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (229.30, 116.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.445 CPU sec, 4.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13611, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 172, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (189.94, 119.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.51, 117.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.183 CPU sec, 4.637 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13352, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (143.90, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (225.40, 114.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.680 CPU sec, 4.534 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13367, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 212, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (208.05, 116.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 192, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (223.26, 113.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.353 CPU sec, 3.702 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13415, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 189, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (181.67, 109.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (226.06, 114.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.077 CPU sec, 4.361 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13351, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 140, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (122.85, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (222.35, 111.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.255 CPU sec, 4.560 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13221, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 282, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (243.54, 163.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 192, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (222.68, 112.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.283 CPU sec, 4.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13133, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 97, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (130.36, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (218.72, 107.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.171 CPU sec, 4.950 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13039, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 177, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 356) [M::mem_pestat] mean and std.dev: (170.76, 55.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (215.83, 106.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.116 CPU sec, 4.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13017, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (147.47, 124.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (221.27, 112.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.800 CPU sec, 4.953 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13771, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 230, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (206.00, 103.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (231.66, 121.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.890 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13730, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 141, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (177.07, 105.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.87, 121.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 102.886 CPU sec, 3.866 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13689, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (144.00, 100.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (230.02, 119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.632 CPU sec, 3.917 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13658, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 159, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (169.58, 88.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (230.59, 119.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.429 CPU sec, 4.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13694, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 150, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (183.29, 96.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.91, 119.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.003 CPU sec, 4.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13614, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 143, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (150.92, 70.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (230.28, 118.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.842 CPU sec, 4.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13603, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 197, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (182.23, 97.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (229.24, 117.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 100.317 CPU sec, 3.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13636, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 95, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (88.89, 54.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (226.91, 117.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.607 CPU sec, 5.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13381, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 125, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (130.36, 83.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (230.11, 118.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.390 CPU sec, 4.564 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13543, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 218, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (199.29, 77.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (229.46, 118.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.341 CPU sec, 4.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13748, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 173, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (186.73, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.10, 122.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.283 CPU sec, 4.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13556, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (196.92, 58.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.73, 122.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.600 CPU sec, 4.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13776, 6, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (239.09, 125.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.620 CPU sec, 5.550 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13679, 5, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.04, 123.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.411 CPU sec, 4.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13687, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 189, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (221.00, 133.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.85, 125.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.061 CPU sec, 4.806 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13634, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 117, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (145.41, 110.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.75, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.041 CPU sec, 4.798 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13627, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 93, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (136.35, 108.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.08, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.599 CPU sec, 4.528 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13797, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 116, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (131.40, 75.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.77, 123.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.065 CPU sec, 5.143 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13759, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (134.00, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.93, 122.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.813 CPU sec, 4.964 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13611, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 145, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (144.33, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.12, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.227 CPU sec, 4.479 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13596, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 141, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (153.00, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.77, 123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.559 CPU sec, 4.543 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13599, 8, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (230.98, 119.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.968 CPU sec, 4.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13129, 4, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.17, 123.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 123.104 CPU sec, 4.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13410, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 146, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (198.94, 135.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.75, 120.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.733 CPU sec, 5.935 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13442, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (166.30, 103.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.82, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.173 CPU sec, 4.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13382, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 114, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (135.85, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.83, 119.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.748 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13483, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 126, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (149.00, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.66, 122.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.955 CPU sec, 4.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13389, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 81, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (116.58, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.99, 120.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.753 CPU sec, 4.567 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13523, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (171.40, 107.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.00, 122.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.440 CPU sec, 4.497 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13572, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (198.17, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (235.07, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.021 CPU sec, 6.104 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13723, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 171, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (148.88, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.76, 123.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.314 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13657, 4, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (236.24, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.068 CPU sec, 5.783 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13571, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 173, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (198.82, 136.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.10, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.897 CPU sec, 4.383 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13524, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 176, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (184.83, 96.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.97, 119.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.196 CPU sec, 4.328 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13644, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 276, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (257.11, 143.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (232.43, 118.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.145 CPU sec, 4.757 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13642, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 164, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (194.15, 143.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.01, 118.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.864 CPU sec, 5.038 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13659, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 251, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (204.33, 101.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.15, 121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.987 CPU sec, 4.147 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13686, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 118, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551) [M::mem_pestat] mean and std.dev: (158.77, 113.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.41, 118.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.025 CPU sec, 4.740 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13814, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 228, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (219.92, 102.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.34, 117.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.051 CPU sec, 4.460 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13716, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 157, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (181.50, 108.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (227.75, 116.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.235 CPU sec, 4.153 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13722, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (140.58, 88.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.89, 116.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.735 CPU sec, 5.406 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13500, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 169, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (178.75, 126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.72, 118.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.101 CPU sec, 4.248 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13573, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (165.53, 114.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.88, 117.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.371 CPU sec, 4.493 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13640, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 147, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (164.53, 112.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.46, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.127 CPU sec, 5.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13703, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 128, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (151.57, 79.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (237.85, 123.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.663 CPU sec, 4.351 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13686, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 114, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (157.46, 109.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.77, 123.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.832 CPU sec, 4.687 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13662, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 255, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (255.00, 100.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.60, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.981 CPU sec, 4.604 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13780, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 207, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (203.33, 109.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.39, 124.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.193 CPU sec, 4.349 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13554, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.49, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.526 CPU sec, 5.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13738, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (236.99, 122.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.549 CPU sec, 4.622 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13749, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 119, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (115.22, 34.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.72, 122.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.350 CPU sec, 4.921 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13724, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 170, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (191.06, 107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.34, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.850 CPU sec, 4.186 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13808, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 136, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (142.00, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.75, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.908 CPU sec, 4.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13591, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (124.22, 78.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.87, 122.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.332 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13784, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 169, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (159.33, 89.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.66, 121.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.073 CPU sec, 5.032 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13366, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 108, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (146.50, 77.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.61, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.450 CPU sec, 4.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13340, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (233.98, 120.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.924 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13393, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 167, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (172.86, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.71, 119.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.752 CPU sec, 5.464 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13455, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 115, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (154.00, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.90, 118.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.726 CPU sec, 4.727 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13568, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 178, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (215.08, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (234.45, 121.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.305 CPU sec, 5.461 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13510, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.53, 120.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.228 CPU sec, 4.795 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13665, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 218, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (218.50, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (233.37, 120.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.046 CPU sec, 4.059 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13624, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (147.47, 64.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.39, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.789 CPU sec, 4.203 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13629, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 129, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (125.53, 55.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.33, 121.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.467 CPU sec, 4.401 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13756, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 200, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (196.50, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.84, 122.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.737 CPU sec, 4.339 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13662, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 183, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (192.45, 81.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.55, 123.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.017 CPU sec, 5.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13899, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (236.16, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.307 CPU sec, 4.738 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13723, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 173, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (208.62, 169.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.76, 119.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.718 CPU sec, 4.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13728, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 167, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (164.10, 96.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.73, 118.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.548 CPU sec, 5.250 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13745, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 187, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (166.71, 105.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (229.65, 117.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.451 CPU sec, 5.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13711, 1, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.48, 119.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.937 CPU sec, 4.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13632, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 143, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (165.93, 99.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (227.29, 118.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.995 CPU sec, 4.546 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13556, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 156, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (157.69, 92.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (225.25, 115.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.325 CPU sec, 4.308 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13416, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 111, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (128.15, 75.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (226.86, 115.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.325 CPU sec, 4.247 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13579, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 197, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (177.44, 68.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (226.43, 116.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.948 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 13364, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 150, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (158.14, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.44, 115.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.626 CPU sec, 4.523 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13504, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 139, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (154.79, 80.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.00, 119.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.005 CPU sec, 3.862 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13624, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 175, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (188.44, 132.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (238.66, 124.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.905 CPU sec, 4.386 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13699, 3, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (239.30, 124.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.983 CPU sec, 4.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13613, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.07, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.785 CPU sec, 4.892 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13672, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 198, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (204.80, 142.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.10, 123.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.925 CPU sec, 4.272 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13541, 6, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.24, 124.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.634 CPU sec, 5.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13666, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 180, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (222.07, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (234.60, 122.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.664 CPU sec, 3.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13617, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 186, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (183.00, 90.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.63, 121.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.458 CPU sec, 5.044 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13734, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (226.73, 112.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.25, 119.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.626 CPU sec, 4.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13793, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 147, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (157.50, 96.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.05, 121.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.228 CPU sec, 5.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13725, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (232.18, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.443 CPU sec, 4.280 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13747, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 162, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.07, 77.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (230.52, 119.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.867 CPU sec, 5.079 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13527, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 146, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (159.17, 84.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.96, 119.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.314 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13144, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 212, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 333) [M::mem_pestat] mean and std.dev: (210.10, 49.02) [M::mem_pestat] low and high boundaries for proper pairs: (14, 406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.16, 120.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.318 CPU sec, 4.995 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13263, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.90, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 136.644 CPU sec, 5.061 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13516, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 126, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (148.00, 65.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.28, 120.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 134.947 CPU sec, 5.057 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13544, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 123, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (137.47, 76.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.59, 123.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.658 CPU sec, 5.288 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13539, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 120, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (128.50, 55.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.43, 121.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.977 CPU sec, 5.207 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13551, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 196, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (176.60, 107.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.68, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.742 CPU sec, 4.577 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13697, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 143, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (173.50, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (236.38, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.313 CPU sec, 5.026 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13720, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 174, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (201.87, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.69, 121.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.263 CPU sec, 4.314 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13721, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 188, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (269.00, 209.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.23, 123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.397 CPU sec, 4.711 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13780, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 170, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (174.81, 89.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (238.55, 124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.459 CPU sec, 4.659 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13596, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.78, 125.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.876 CPU sec, 5.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13446, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 109, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (113.93, 59.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (225.67, 114.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.897 CPU sec, 4.682 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13588, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 164, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (181.79, 100.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (230.32, 117.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.042 CPU sec, 4.297 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13656, 6, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (229.30, 117.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.115 CPU sec, 5.097 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13844, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 116, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (154.18, 92.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (228.81, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.078 CPU sec, 4.930 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13693, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 187, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (152.33, 61.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (228.61, 118.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.150 CPU sec, 3.997 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13509, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 200, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (205.14, 103.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.09, 117.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.758 CPU sec, 4.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13564, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 149, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (148.79, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (227.55, 116.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.886 CPU sec, 4.346 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13589, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 121, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (148.93, 94.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (227.87, 116.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.354 CPU sec, 4.399 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13343, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (221.77, 93.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (227.68, 116.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.040 CPU sec, 3.954 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13572, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 231, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (237.27, 119.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.37, 117.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.472 CPU sec, 4.582 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13597, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 144, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (155.25, 67.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.78, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.255 CPU sec, 5.415 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 13577, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 139, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (159.23, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.62, 119.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.676 CPU sec, 5.240 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13580, 6, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 163, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (159.24, 51.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.82, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.957 CPU sec, 5.199 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13670, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.59, 119.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.000 CPU sec, 4.643 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13651, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 145, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (171.60, 86.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (232.38, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.985 CPU sec, 4.539 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13637, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 199, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (229.50, 164.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.35, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.383 CPU sec, 6.068 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13549, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 126, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (141.63, 85.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.03, 120.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.408 CPU sec, 5.254 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13698, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (239.67, 90.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.53, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.597 CPU sec, 6.030 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13602, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (140.43, 92.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.26, 119.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.816 CPU sec, 4.219 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13542, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 145, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (137.27, 68.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (231.88, 120.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.337 CPU sec, 4.376 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13614, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 137, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (172.82, 101.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.43, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.753 CPU sec, 4.110 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13408, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 120, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (132.93, 93.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (229.17, 117.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.230 CPU sec, 4.685 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13267, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 169, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496) [M::mem_pestat] mean and std.dev: (142.23, 72.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.14, 113.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.842 CPU sec, 4.459 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13161, 3, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (224.19, 113.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 111.633 CPU sec, 3.815 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13411, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 181, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (170.12, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (226.58, 115.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.720 CPU sec, 4.321 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13405, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.15, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.083 CPU sec, 4.570 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13426, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 147, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (152.38, 89.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (227.97, 117.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.153 CPU sec, 5.015 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13551, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 190, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (155.11, 59.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (230.43, 118.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.798 CPU sec, 6.491 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13769, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 178, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (186.90, 79.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.19, 120.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.496 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13540, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 151, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (177.33, 100.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (233.01, 119.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.317 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13516, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 95, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (109.57, 52.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.17, 120.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.471 CPU sec, 4.873 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13723, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (198.50, 86.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.49, 119.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.766 CPU sec, 4.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13657, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 131, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (150.68, 78.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (231.87, 121.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.689 CPU sec, 4.265 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13706, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 260, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (225.07, 123.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (230.20, 117.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.818 CPU sec, 4.129 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13770, 1, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (228.96, 117.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 114.060 CPU sec, 4.850 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13594, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 143, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 220) [M::mem_pestat] mean and std.dev: (133.73, 19.46) [M::mem_pestat] low and high boundaries for proper pairs: (34, 251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (228.61, 118.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.462 CPU sec, 4.422 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13769, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 270, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (208.55, 115.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (227.35, 116.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.236 CPU sec, 5.562 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13703, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 168, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (186.12, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.52, 117.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.494 CPU sec, 4.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13538, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 233, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (795.08, 1094.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (227.51, 115.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.122 CPU sec, 4.188 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13553, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 104, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 337) [M::mem_pestat] mean and std.dev: (129.00, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (227.63, 115.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.701 CPU sec, 4.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13498, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 157, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (139.50, 77.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (226.63, 115.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.430 CPU sec, 5.587 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13623, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 184, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (174.69, 95.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (228.69, 116.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.841 CPU sec, 4.771 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13467, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 107, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (113.67, 63.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.15, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.161 CPU sec, 4.684 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13523, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 139, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (155.12, 112.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.56, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.527 CPU sec, 4.885 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13614, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.30, 122.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 114.654 CPU sec, 4.295 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13730, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.39, 122.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.920 CPU sec, 5.050 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13686, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (161.60, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.92, 121.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.156 CPU sec, 4.512 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13678, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 232, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (201.07, 140.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (232.47, 120.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.933 CPU sec, 4.285 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13769, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 229, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (203.36, 100.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.49, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.905 CPU sec, 4.559 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13710, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 149, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (161.00, 110.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.15, 121.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.534 CPU sec, 5.369 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13605, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 173, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (179.71, 93.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.48, 118.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.577 CPU sec, 4.232 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13601, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 173, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 314) [M::mem_pestat] mean and std.dev: (155.40, 49.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.83, 121.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.264 CPU sec, 4.502 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13701, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 134, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (153.18, 78.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.09, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.861 CPU sec, 3.889 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13695, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 118, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (118.56, 48.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.45, 119.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 105.598 CPU sec, 4.664 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13601, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.13, 120.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.706 CPU sec, 5.125 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13168, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (224.78, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.665 CPU sec, 4.763 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13156, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 119, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (139.47, 83.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (224.01, 113.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.536 CPU sec, 3.977 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13269, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 156, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (180.00, 110.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (227.10, 116.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.804 CPU sec, 4.426 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13517, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 137, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (142.25, 61.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (226.21, 114.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.183 CPU sec, 4.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13618, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 145, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (128.56, 74.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (228.00, 116.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.886 CPU sec, 4.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13646, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 134, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (137.32, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (225.66, 115.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.058 CPU sec, 5.597 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13542, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 166, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (191.56, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.40, 118.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.000 CPU sec, 4.540 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13476, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 104, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (113.00, 50.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (230.20, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.724 CPU sec, 4.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13620, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 78, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (131.23, 100.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.54, 118.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.002 CPU sec, 5.708 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13699, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 154, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (182.62, 102.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (232.33, 119.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.932 CPU sec, 5.407 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13705, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (233.70, 121.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.073 CPU sec, 4.261 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13603, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 143, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (156.76, 107.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.42, 120.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.509 CPU sec, 5.443 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13637, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 127, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (159.09, 118.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (228.63, 117.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.586 CPU sec, 4.340 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13553, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 204, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (168.55, 57.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (225.54, 114.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.470 CPU sec, 4.429 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13620, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 139, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (158.17, 111.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (226.69, 115.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.935 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13431, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (225.49, 114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.274 CPU sec, 4.650 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13653, 2, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (227.57, 115.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 111.937 CPU sec, 4.225 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13720, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (139.67, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (225.46, 114.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.405 CPU sec, 6.198 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13464, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 206, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (200.33, 118.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (224.69, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.481 CPU sec, 4.599 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13304, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 167, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (156.17, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (226.90, 114.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.536 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13500, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 120, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (117.56, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (224.21, 113.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 106.947 CPU sec, 4.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13691, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 134, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (130.69, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.53, 117.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.459 CPU sec, 4.731 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13717, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (157.61, 95.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.84, 120.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.767 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13744, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.56, 122.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.361 CPU sec, 4.672 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13548, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 179, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (174.10, 85.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (231.39, 120.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.709 CPU sec, 4.989 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13798, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 194, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (167.42, 79.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.41, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.997 CPU sec, 4.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13569, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 210, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (181.27, 108.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.78, 120.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.335 CPU sec, 5.585 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13598, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (230.01, 118.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.887 CPU sec, 4.162 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13557, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 150, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (167.80, 119.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (230.75, 119.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.700 CPU sec, 4.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13755, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 143, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (168.84, 88.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (228.75, 118.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.615 CPU sec, 5.005 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13433, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 137, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2165) [M::mem_pestat] mean and std.dev: (460.91, 603.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (231.73, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.762 CPU sec, 4.054 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13620, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (228.66, 117.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 112.851 CPU sec, 3.857 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13625, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 157, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (198.77, 135.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (227.36, 116.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.861 CPU sec, 4.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13380, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 167, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (147.07, 76.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (225.98, 116.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.077 CPU sec, 4.486 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13345, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 196, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (194.17, 78.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (227.80, 117.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.199 CPU sec, 4.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13432, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 137, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (157.80, 99.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (226.16, 115.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.499 CPU sec, 4.432 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13294, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (229.77, 118.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.961 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13299, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 185, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (196.15, 113.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 192, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (226.55, 117.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.858 CPU sec, 4.489 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13431, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 216, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (205.42, 89.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.43, 117.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.183 CPU sec, 4.556 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13597, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 141, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (150.50, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.26, 120.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.487 CPU sec, 5.234 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13449, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 169, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (176.25, 117.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (231.24, 120.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.544 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13533, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 154, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (191.25, 106.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.90, 121.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.826 CPU sec, 4.634 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13529, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 179, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (156.06, 65.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.99, 121.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.795 CPU sec, 4.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13643, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 172, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (166.33, 122.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (233.95, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.479 CPU sec, 4.770 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13573, 5, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 107, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (96.12, 36.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.68, 121.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.978 CPU sec, 4.418 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13493, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 188, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (159.79, 103.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.67, 118.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.474 CPU sec, 4.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13661, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 143, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (121.56, 47.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.20, 116.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.996 CPU sec, 5.168 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13410, 7, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (226.49, 115.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.619 CPU sec, 4.326 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13499, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 196, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (180.33, 117.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (225.95, 115.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.928 CPU sec, 4.666 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13285, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 100, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (134.33, 79.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (224.20, 113.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.878 CPU sec, 4.816 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13252, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 204, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (206.25, 91.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 191, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (222.40, 111.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.901 CPU sec, 4.852 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13360, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 87, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (98.20, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (221.31, 110.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.843 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13125, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 178, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (159.71, 58.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 190, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (222.89, 113.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.837 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13085, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 105, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (136.38, 95.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (223.75, 113.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.275 CPU sec, 4.698 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13042, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 116, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (142.33, 87.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (224.79, 113.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.179 CPU sec, 5.114 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13318, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 162, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (169.05, 87.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.09, 120.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.189 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13639, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 178, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (250.08, 199.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.31, 124.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.728 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13714, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (155.39, 105.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (238.14, 123.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.972 CPU sec, 4.710 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13684, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 156, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (174.36, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.79, 122.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.069 CPU sec, 5.735 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13733, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (132.24, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (233.16, 121.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.115 CPU sec, 4.119 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13709, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 208, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (187.50, 94.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.33, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.297 CPU sec, 4.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13665, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 164, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (172.91, 87.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (231.71, 120.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.064 CPU sec, 4.890 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13565, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (42, 124, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (109.89, 66.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.17, 121.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.855 CPU sec, 4.571 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13550, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 150, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (169.31, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.18, 120.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.171 CPU sec, 4.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13709, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 109, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (131.56, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.49, 121.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.509 CPU sec, 5.227 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13658, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 192, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (149.08, 85.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.05, 121.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.302 CPU sec, 4.438 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13579, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 119, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (141.25, 85.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.95, 118.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.065 CPU sec, 4.409 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13587, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 146, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (140.20, 59.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.61, 122.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.907 CPU sec, 4.499 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13709, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 176, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (210.54, 118.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (237.32, 122.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.112 CPU sec, 4.558 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13812, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (98, 226, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (210.50, 112.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (235.57, 121.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.641 CPU sec, 5.484 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13666, 4, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 68, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (108.00, 96.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.24, 125.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.894 CPU sec, 4.686 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13666, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 149, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (179.10, 119.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (238.10, 124.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.904 CPU sec, 4.625 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13566, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (220.00, 65.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.78, 123.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.488 CPU sec, 5.906 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13479, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 118, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (97.08, 32.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.14, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.687 CPU sec, 4.213 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13738, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 149, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (180.08, 89.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (239.02, 124.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.058 CPU sec, 4.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13570, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 238, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (233.00, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.47, 122.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.307 CPU sec, 5.190 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13770, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 128, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (182.93, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (236.76, 122.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.584 CPU sec, 6.249 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13601, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 226, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (201.92, 107.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.78, 121.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.610 CPU sec, 4.020 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13800, 7, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (170.00, 116.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.53, 121.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.172 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13725, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 202, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (177.38, 101.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.63, 119.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.515 CPU sec, 4.329 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13572, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (131.50, 64.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619) [M::mem_pestat] mean and std.dev: (226.20, 115.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.650 CPU sec, 4.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13539, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 123, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (156.36, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (226.09, 115.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.406 CPU sec, 4.616 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13490, 7, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 106, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (116.76, 72.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (227.15, 116.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.145 CPU sec, 4.715 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13289, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 186, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (173.00, 98.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (226.72, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.503 CPU sec, 5.293 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13231, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 154, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (159.46, 72.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 193, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (223.89, 112.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.980 CPU sec, 4.579 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 12931, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (175.07, 89.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (223.47, 111.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.051 CPU sec, 4.477 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12959, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 177, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (166.43, 78.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (223.34, 113.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 137.988 CPU sec, 5.123 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 12755, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 112, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (130.25, 68.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (224.96, 113.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 139.872 CPU sec, 5.211 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13189, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 139, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (124.89, 59.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (234.01, 119.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.473 CPU sec, 5.367 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13668, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (181.40, 76.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (240.43, 125.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.749 CPU sec, 4.552 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13749, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 123, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (146.43, 90.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (236.55, 122.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.156 CPU sec, 5.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13754, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 107, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (152.82, 93.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (237.20, 124.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.135 CPU sec, 5.592 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13620, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 165, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (172.08, 86.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (238.36, 124.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.635 CPU sec, 4.756 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13824, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 89, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (130.21, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 204, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (238.08, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.397 CPU sec, 4.641 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13756, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.43, 121.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.162 CPU sec, 5.668 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13721, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (178.58, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.95, 123.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.761 CPU sec, 4.608 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13745, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 245, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (238.91, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.87, 121.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.857 CPU sec, 4.424 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13742, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 161, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (186.95, 114.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (235.39, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.972 CPU sec, 4.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13691, 7, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 181, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (157.50, 94.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (233.60, 119.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.681 CPU sec, 4.744 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13703, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 229, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (187.27, 106.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.44, 120.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.310 CPU sec, 4.060 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13548, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 164, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (180.00, 100.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.23, 122.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.326 CPU sec, 5.133 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13769, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 185, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 395) [M::mem_pestat] mean and std.dev: (176.00, 87.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 203, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (239.56, 124.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.097 CPU sec, 4.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13637, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 131, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (194.92, 155.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (238.51, 124.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.566 CPU sec, 4.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13545, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 142, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (170.25, 111.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (238.54, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.303 CPU sec, 5.170 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13485, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (106, 186, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (182.00, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (236.95, 123.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.392 CPU sec, 4.764 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13709, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 135, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (142.00, 70.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (238.14, 124.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.120 CPU sec, 4.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13716, 8, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 202, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (186.69, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.55, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.284 CPU sec, 4.563 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13632, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 160, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (156.06, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (237.90, 125.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.408 CPU sec, 4.347 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13731, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 89, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (131.41, 91.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (236.23, 121.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 131.616 CPU sec, 6.298 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13732, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 170, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (208.00, 95.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (236.95, 123.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.411 CPU sec, 4.768 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13743, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 104, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (132.07, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (237.14, 124.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.046 CPU sec, 4.957 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13733, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 171, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (168.33, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.31, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.992 CPU sec, 4.654 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13589, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 89, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (126.17, 84.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (229.48, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.700 CPU sec, 4.467 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13575, 1, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 87, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (86.80, 37.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.72, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.464 CPU sec, 4.633 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13579, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 175, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (192.38, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (228.34, 116.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.300 CPU sec, 4.753 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13494, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 135, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (163.22, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (225.55, 116.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.446 CPU sec, 4.373 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 13415, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 119, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (125.62, 61.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 192, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (225.55, 115.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.130 CPU sec, 4.299 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13407, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 162, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (155.78, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (227.84, 116.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.326 CPU sec, 4.526 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13252, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (226.37, 114.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.006 CPU sec, 4.901 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13069, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 157, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (159.00, 104.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 194, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (226.59, 114.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.065 CPU sec, 4.945 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13124, 4, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 198, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (189.36, 87.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 193, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (226.59, 114.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.533 CPU sec, 5.270 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12791, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 222, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (179.60, 70.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (227.72, 116.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.832 CPU sec, 5.553 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13551, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 164, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (175.07, 102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.43, 122.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 133.220 CPU sec, 5.016 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13703, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 195, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (189.67, 94.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.24, 123.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.214 CPU sec, 4.410 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 14043, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 114, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (130.27, 58.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.44, 124.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.889 CPU sec, 4.482 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13783, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 177, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (226.78, 148.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 202, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (235.15, 122.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.116 CPU sec, 4.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13793, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 173, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (164.09, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (235.55, 121.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.197 CPU sec, 4.320 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13851, 5, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 245, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (256.67, 165.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.10, 121.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.768 CPU sec, 5.691 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13702, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (127.93, 62.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (233.47, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.611 CPU sec, 4.867 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13763, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 116, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (142.35, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.35, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.758 CPU sec, 4.986 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13807, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 145, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (173.62, 71.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (233.90, 120.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.664 CPU sec, 4.902 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13793, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 259, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (202.10, 111.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (235.15, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.763 CPU sec, 4.313 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13788, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 167, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (165.75, 95.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.29, 123.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.736 CPU sec, 3.961 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13833, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 211, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (208.27, 121.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.43, 123.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.752 CPU sec, 4.080 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13517, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (143.27, 79.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (236.33, 122.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.881 CPU sec, 4.678 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13747, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 124, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (162.36, 80.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 202, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (236.90, 122.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.361 CPU sec, 5.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13674, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 134, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (152.78, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (237.90, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.971 CPU sec, 4.501 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13734, 2, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 168, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (145.24, 84.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.52, 123.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.963 CPU sec, 4.677 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13658, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 129, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (179.55, 127.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.73, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.512 CPU sec, 4.574 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 13719, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 133, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (147.00, 74.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 203, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (236.26, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.839 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13746, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 249, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (247.73, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (237.51, 122.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.257 CPU sec, 4.705 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13804, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 107, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (127.12, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (235.94, 122.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.360 CPU sec, 4.183 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13777, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 211, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (190.92, 87.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (237.22, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.819 CPU sec, 4.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13717, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (129.65, 83.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.90, 121.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.947 CPU sec, 4.541 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13672, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 196, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (213.44, 140.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 203, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (237.18, 123.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.748 CPU sec, 4.635 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13613, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 145, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (181.94, 120.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (223.67, 113.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.900 CPU sec, 4.341 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13568, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (100, 156, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (155.38, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 192, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (224.90, 113.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 108.244 CPU sec, 4.098 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13495, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 131, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (120.62, 65.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (223.79, 113.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.403 CPU sec, 4.612 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13507, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 212, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (203.85, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (221.41, 111.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.541 CPU sec, 4.400 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13636, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 93, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (93.90, 46.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (221.65, 112.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.010 CPU sec, 4.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13422, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 137, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (153.00, 56.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (221.23, 111.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.357 CPU sec, 4.260 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13105, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 177, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (162.17, 99.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 190, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (220.84, 110.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.785 CPU sec, 5.231 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13047, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 167, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (175.55, 81.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (217.11, 106.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.244 CPU sec, 6.259 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 12942, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 186, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (170.72, 68.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 186, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (214.62, 105.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.116 CPU sec, 5.527 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 12920, 1, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 104, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (93.58, 43.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (215.48, 107.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 132.312 CPU sec, 5.317 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13632, 7, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (228.82, 118.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 128.199 CPU sec, 4.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13730, 2, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (231.67, 120.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 127.672 CPU sec, 4.724 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13567, 0, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 140, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (144.42, 86.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (233.60, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.711 CPU sec, 4.590 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13625, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 165, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (148.77, 97.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.10, 120.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.428 CPU sec, 4.524 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13803, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 298, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (192.00, 102.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.58, 120.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.687 CPU sec, 4.384 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13615, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 151, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (141.65, 69.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.55, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.239 CPU sec, 5.405 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13653, 0, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 197, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (164.85, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.00, 119.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.374 CPU sec, 4.762 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13640, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 179, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (183.07, 99.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (232.02, 120.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.648 CPU sec, 6.041 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13475, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (231.02, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.449 CPU sec, 4.509 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13693, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 157, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (162.56, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (229.65, 118.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.029 CPU sec, 4.451 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13606, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 151, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (159.20, 100.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.04, 119.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.370 CPU sec, 5.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13604, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 99, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (94.27, 26.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.06, 120.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.357 CPU sec, 4.318 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 13613, 2, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (235.41, 121.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 118.816 CPU sec, 4.581 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 13588, 6, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 169, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (163.71, 58.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.52, 120.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.691 CPU sec, 4.596 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13587, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 173, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (160.14, 90.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (234.72, 120.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.484 CPU sec, 3.849 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13627, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 124, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (145.08, 89.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.92, 120.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.333 CPU sec, 4.436 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13736, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 96, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (115.44, 119.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.58, 121.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.477 CPU sec, 4.984 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13565, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 116, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (186.91, 152.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (234.82, 121.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 111.547 CPU sec, 4.179 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13533, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 160, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (172.67, 95.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (234.38, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.054 CPU sec, 4.660 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13773, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 136, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (100.11, 43.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 197, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (233.11, 121.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.575 CPU sec, 5.573 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13422, 1, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 122, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (151.85, 100.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.02, 122.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 125.221 CPU sec, 5.146 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13517, 4, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 164, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (144.58, 76.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (234.12, 120.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 130.483 CPU sec, 5.262 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13470, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 103, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (95.60, 39.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (233.57, 120.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.727 CPU sec, 4.289 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13604, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (104, 149, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (153.93, 83.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (225.06, 114.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.135 CPU sec, 4.152 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13558, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (225.15, 113.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 116.316 CPU sec, 4.395 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13543, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 160, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (125.10, 39.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (222.22, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.214 CPU sec, 5.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13469, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 209, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (197.33, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 190, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (222.50, 112.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 109.083 CPU sec, 3.758 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13364, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (103, 177, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (177.29, 107.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 191, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (220.06, 108.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.092 CPU sec, 4.256 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13300, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 185, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (175.63, 100.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 189, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (220.35, 111.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.682 CPU sec, 4.301 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13253, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 220, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (197.38, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (135, 186, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (215.58, 107.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 715) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.222 CPU sec, 5.939 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 12958, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (105, 189, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (192.29, 102.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (214.89, 107.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 135.351 CPU sec, 5.176 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 12748, 1, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 267, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (233.10, 130.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (216.00, 106.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 141.089 CPU sec, 5.224 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 12897, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (99, 153, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (158.92, 79.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (217.73, 107.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.756 CPU sec, 5.134 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13423, 3, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.67, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 126.367 CPU sec, 5.639 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13818, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 159, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (180.92, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (233.81, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.737 CPU sec, 4.310 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13762, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 82, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (91.67, 82.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (233.79, 122.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.046 CPU sec, 4.658 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13673, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 112, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (166.64, 126.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.52, 121.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.663 CPU sec, 4.630 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13653, 5, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.70, 121.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 120.454 CPU sec, 5.212 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13693, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 133, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (170.67, 128.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (232.36, 119.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.595 CPU sec, 5.356 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13682, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 172, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (160.31, 88.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (232.71, 120.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.169 CPU sec, 4.575 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13604, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 181, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (192.87, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (230.20, 117.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 124.540 CPU sec, 4.807 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13652, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (107, 156, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (190.80, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (229.52, 118.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.376 CPU sec, 4.102 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13446, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 186, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (168.94, 110.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 196, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (230.89, 119.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.605 CPU sec, 4.156 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13736, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (228.37, 116.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 115.603 CPU sec, 4.368 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13733, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 146, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (141.54, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (233.36, 121.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.859 CPU sec, 4.646 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13558, 2, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 144, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (166.71, 90.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.70, 120.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 127.099 CPU sec, 4.588 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 13635, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.79, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 119.245 CPU sec, 4.877 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13562, 3, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 202, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.82, 121.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 122.113 CPU sec, 5.334 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13521, 6, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 127, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (143.67, 89.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (234.26, 120.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.065 CPU sec, 4.601 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13768, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 259, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (229.60, 106.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (235.14, 122.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 122.569 CPU sec, 5.040 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13786, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 205, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (212.33, 166.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 199, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.31, 121.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.556 CPU sec, 4.530 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13618, 7, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 106, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (125.70, 84.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (236.06, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 128.374 CPU sec, 4.897 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13628, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 194, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (167.87, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (233.95, 121.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.937 CPU sec, 4.618 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13665, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (98.12, 41.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.32, 121.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.441 CPU sec, 4.286 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13712, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 174, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (317.70, 307.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (233.48, 120.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.596 CPU sec, 4.782 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13635, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 252, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (229.93, 112.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (235.03, 122.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 129.514 CPU sec, 5.642 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13455, 5, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (229.43, 117.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.927 CPU sec, 4.804 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13700, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 204, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (188.70, 89.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (228.61, 117.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 110.748 CPU sec, 4.021 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13482, 5, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (226.98, 116.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 124.084 CPU sec, 5.538 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13486, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 174, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (241.77, 157.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (227.02, 114.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 112.441 CPU sec, 4.765 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13713, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 153, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (174.00, 107.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 194, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (225.93, 116.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 118.165 CPU sec, 4.519 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13562, 1, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 195, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (253.00, 261.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (222.01, 112.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.028 CPU sec, 4.669 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13344, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (138.92, 69.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (220.28, 110.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.806 CPU sec, 4.487 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13333, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 193, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (194.25, 86.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (136, 188, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (219.10, 109.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.566 CPU sec, 4.695 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 13256, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (91, 163, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (146.74, 73.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 191, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (222.19, 111.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.914 CPU sec, 4.510 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13084, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 92, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (146.82, 129.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (137, 189, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (219.59, 109.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.738 CPU sec, 4.723 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13511, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (159.67, 132.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 195, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (227.47, 116.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 126.331 CPU sec, 4.759 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13805, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 211, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (187.50, 107.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.47, 122.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.757 CPU sec, 4.380 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13537, 2, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 102, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (183.94, 179.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 201, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (235.30, 121.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 116.762 CPU sec, 4.067 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13710, 5, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 174, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (155.27, 81.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (234.78, 123.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.319 CPU sec, 4.423 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13722, 3, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 121, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (134.18, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (232.91, 120.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.664 CPU sec, 4.746 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 13786, 2, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 218, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (179.75, 88.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (233.84, 121.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.866 CPU sec, 5.223 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13785, 4, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (95, 156, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (156.83, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.49, 120.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.206 CPU sec, 3.874 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 13707, 5, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 131, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (126.07, 84.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (231.40, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 123.388 CPU sec, 5.394 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13840, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 241, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (209.55, 108.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.76, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.566 CPU sec, 4.823 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13839, 1, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 113, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (144.29, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (231.81, 119.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.509 CPU sec, 4.431 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 13827, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 153, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (146.54, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (230.78, 118.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 121.581 CPU sec, 5.752 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 13658, 3, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (133.53, 78.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (231.49, 119.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.423 CPU sec, 4.472 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 13897, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 196, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (159.00, 45.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 198, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.60, 120.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 113.553 CPU sec, 4.439 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13587, 3, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 167, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (180.09, 78.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 199, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (232.52, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 120.697 CPU sec, 5.137 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 13642, 0, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 193, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (171.00, 93.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (234.47, 120.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.149 CPU sec, 4.013 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 13764, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (234.97, 121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 121.742 CPU sec, 4.508 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 13719, 3, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (142.12, 81.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 200, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (233.80, 121.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.800 CPU sec, 4.258 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 13526, 4, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 200, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (235.49, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 66226 reads in 117.369 CPU sec, 4.926 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13786, 4, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 162, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (170.40, 108.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 200, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (235.95, 123.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 114.754 CPU sec, 4.516 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 13804, 5, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 162, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (174.27, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 197, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (230.43, 118.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 117.264 CPU sec, 4.413 real sec [M::process] read 66226 sequences (10000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 13668, 2, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 187, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (179.63, 78.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (233.57, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 119.945 CPU sec, 5.440 real sec [M::process] read 54410 sequences (8215910 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 13698, 6, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 138, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (133.67, 56.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 198, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (232.81, 121.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 66226 reads in 115.416 CPU sec, 4.028 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 11375, 4, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (233.84, 120.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 54410 reads in 94.264 CPU sec, 3.872 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 30 -R @RG\tID:21191\tSM:UG10S2P652\tLB:lib1\tPL:ILLUMINA -w 3 -K 10000000 -c 10000 /workdir/nisha/reference_V7/cassavaV7_chrAndScaffoldsCombined_numeric.fa 5347_7332_21191_HLKNYBGXX_UG10S2P652_R1.fastq.gz 5347_7332_21191_HLKNYBGXX_UG10S2P652_R2.fastq.gz [main] Real time: 3834.431 sec; CPU: 97185.516 sec Finished alignement of UG10S2P652 at Thu Apr 4 02:26:15 2019 Sorting BAM file started at Thu Apr 4 02:26:15 2019 samtools sort -@ 30 -m 4G UG10S2P652_unsorted.bam -o UG10S2P652.bam [bam_sort_core] merging from 0 files and 30 in-memory blocks... Finished at Thu Apr 4 02:26:15 2019 rm UG10S2P652_unsorted.bam rm 5347_7332_21191_HLKNYBGXX_UG10S2P652_R1.fastq.gz rm 5347_7332_21191_HLKNYBGXX_UG10S2P652_R2.fastq.gz samtools index UG10S2P652.bam Finished BAM file generation of the sample - UG10S2P652